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1.
Eur J Med Chem ; 216: 113322, 2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33652353

RESUMO

In this paper, the 2,5-disubstituted furan derivatives containing 1,3,4-thiadiazole were synthesized and screened for their inhibitory activity against α-glucosidase and ß-glucuronidases to obtain potent α-glucosidase inhibitor 9 (IC50 = 0.186 µM) and E. coli ß-glucuronidase inhibitor 26 (IC50 = 0.082 µM), respectively. The mechanisms of the compounds were studied. The kinetic study revealed that compound 9 is a competitive inhibitor against α-glucosidase (Ki = 0.05 ± 0.003 µM) and molecular docking simulation showed several key interactions between 9 and the target including hydrogen bond and p-π stacking interaction. Derivative 26 (Ki = 0.06 ± 0.005 µM) displayed uncompetitive inhibition behavior against EcGUS. Furthermore, the result of docking revealed the furan ring of 26 may be a key moiety in obstructing the active domain of EcGUS. In addition, compound 15 exhibited significant inhibitory activity against these two enzymes, with potential therapeutic effects against diabetes and against CPT-11-induced diarrhea. At the same time, their low toxicity against normal liver tissue LO2 cells lays the foundation for in vivo studies and the development of bifunctional drug.

2.
J Cell Mol Med ; 2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33655701

RESUMO

CLEC10A, (C-type lectin domain family 10, member A), as the member of C-type lectin receptors (CLRs), plays a vital role in modulating innate immunity and adaptive immunity and has shown great potential as an immunotherapy target for cancers. However, there is no functional research of CLEC10A in prognostic risk, immunotherapy or any other treatment of lung adenocarcinoma (LUAD). We performed bioinformatics analysis on LUAD data downloaded from TCGA (The Cancer Genome Atlas) and GEO (Gene Expression Omnibus), and jointly analysed with online databases such as HPA, LinkedOmics, TIMER, ESTIMATE and TISIDB. We found that lower expression of CLEC10A was accompanied with worse outcomes of LUAD patients. Moreover, CLEC10A expression was significantly correlated with a variety of the tumour-infiltrating immune cells (TIICs). As a promising prognosis predictor and potential immunotherapy target, the potential influence and mechanisms of CLEC10A in LUAD deserve further exploring.

3.
Eur J Pharmacol ; : 173975, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33647258

RESUMO

Natural products are a large source of clinically effective antitumor drugs. Millepachine is a natural product derived from leguminous plants that was reported to display antitumor activity. In this study, the novel compound, (1H-indol-5-yl)(5-methoxy-2,2-dimethyl-2H-chromen-8-yl)methanone (MIL-1), was designed and synthesized by fusing millepachine and indole rings. MIL-1 exerted much better antitumor activity than millepachine, manifesting as a 24- to 201-fold increase in vitro cytotoxicity and a 2.4-fold increase in in vivo antitumor activity in hepatocellular cell lines-derived models. The immunofluorescence and HPLC detection revealed that MIL-1 was a potent microtubule targeting agent by interfering with the equilibrium of tubulin-microtubule dynamics and irreversibly binding to tubulin. MIL-1 displayed remarkable antitumor activity with an IC50 of 31-207 nM towards various human cancer cell lines derived from various organs and tissues, and it exerted no evidence of toxicity against normal cells. Mechanistic studies showed that MIL-1 arrested the cell cycle at G2/M phase and induced apoptosis by activating caspase-3 activity and reactive oxygen species (ROS) accumulation. Nevertheless, the superior antitumor effect of MIL-1 is worthy of further detailed study for the treatment of hepatocellular carcinoma (HCC).

4.
Technol Cancer Res Treat ; 20: 1533033821989817, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33550923

RESUMO

BACKGROUND: Prostate cancer is a common malignant tumor with a high incidence. MicroRNAs (miRNAs) have been shown to be important post-transcriptional regulators during tumorigenesis. This study aimed to explore the effect of miR-144 on PCa proliferation and apoptosis. MATERIAL AND METHODS: The expression of miR-144 and EZH2 were examined in clinical PCa tissues. PCa cell line LNCAP and DU-145 was employed and transfected with miR-144 mimics or inhibitors. The correlation between miR-144 and EZH2 was verified by luciferase reporter assay. Cell viability, apoptosis and migratory capacity were detected by CCK-8, flow cytometry assay and wound healing assay. The protein level of EZH2, E-Cadherin, N-Cadherin and vimentin were analyzed by western blotting. RESULTS: miR-144 was found to be negatively correlated to the expression of EZH2 in PCa tissues. Further studies identified EZH2 as a direct target of miR-144. Moreover, overexpression of miR-144 downregulated expression of EZH2, reduced cell viability and promoted cell apoptosis, while knockdown of miR-144 led to an inverse result. miR-144 also suppressed epithelial-mesenchymal transition level of PCa cells. CONCLUSION: Our study indicated that miR-144 negatively regulate the expression of EZH2 in clinical specimens and in vitro. miR-144 can inhibit cell proliferation and induce cell apoptosis in PCa cells. Therefore, miR-144 has the potential to be used as a biomarker for predicting the progression of PCa.

5.
Food Chem ; 350: 129245, 2021 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-33601091

RESUMO

The co-occurrence of multiple pesticides in wheat fields adversely affects human health and the environment. Herein, 206 pairs of wheat and soil samples were collected from wheat fields in Beijing, China from 2018 to 2020. One or multiple pesticide residues were detected, and carbendazim (maximum: 38511.5 µg/kg) and tebuconazole (maximum: 45.4 µg/kg) had heavy occurrence in the wheat samples. Carbendazim, triazoles, and neonicotinoids were frequently detected in the soil samples. HCHs and DDTs were detected, with p,p'-DDE in 100.0% of the soil samples at a maximum concentration of 546.0 µg/kg in 2020. Concentrations of carbendazim, tebuconazole, hexaconazole, and cyhalothrin in the paired soil and wheat samples exhibited significant positive correlations. Pesticides that exceeded the maximum residue limits do not pose non-carcinogenic risks, with one exception. The results provide important references towards risk monitoring and control in wheat fields, as well as facilitating the scientific and reasonable use of these pesticides.

6.
BMC Genomics ; 22(1): 138, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33637038

RESUMO

BACKGROUND: Chloroplast genome resources can provide useful information for the evolution of plant species. Tea plant (Camellia sinensis) is among the most economically valuable member of Camellia. Here, we determined the chloroplast genome of the first natural triploid Chinary type tea ('Wuyi narcissus' cultivar of Camellia sinensis var. sinensis, CWN) and conducted the genome comparison with the diploid Chinary type tea (Camellia sinensis var. sinensis, CSS) and two types of diploid Assamica type teas (Camellia sinensis var. assamica: Chinese Assamica type tea, CSA and Indian Assamica type tea, CIA). Further, the evolutionary mechanism of the chloroplast genome of Camellia sinensis and the relationships of Camellia species based on chloroplast genome were discussed. RESULTS: Comparative analysis showed the evolutionary dynamics of chloroplast genome of Camellia sinensis were the repeats and insertion-deletions (indels), and distribution of the repeats, indels and substitutions were significantly correlated. Chinese tea and Indian tea had significant differences in the structural characteristic and the codon usage of the chloroplast genome. Analysis of sequence characterized amplified region (SCAR) using sequences of the intergenic spacers (trnE/trnT) showed none of 292 different Camellia sinensis cultivars had similar sequence characteristic to triploid CWN, but the other four Camellia species did. Estimations of the divergence time showed that CIA diverged from the common ancestor of two Assamica type teas about 6.2 Mya (CI: 4.4-8.1 Mya). CSS and CSA diverged to each other about 0.8 Mya (CI: 0.4-1.5 Mya). Moreover, phylogenetic clustering was not exactly consistent with the current taxonomy of Camellia. CONCLUSIONS: The repeat-induced and indel-induced mutations were two important dynamics contributed to the diversification of the chloroplast genome in Camellia sinensis, which were not mutually exclusive. Chinese tea and Indian tea might have undergone different selection pressures. Chloroplast transfer occurred during the polyploid evolution in Camellia sinensis. In addition, our results supported the three different domestication origins of Chinary type tea, Chinese Assamica type tea and Indian Assamica type tea. And, the current classification of some Camellia species might need to be further discussed.

7.
Bioresour Technol ; 328: 124847, 2021 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-33609883

RESUMO

Sewage sludge contains various classes of organic pollutants, limiting its land application. Sludge composting can effectively remove some organic pollutants. This review summarizesrecent researches on concentration changes and dissipation of different organic pollutants including persistent organic pollutants during sludge composting, and discusses their dissipation pathways and the current understanding on dissipation mechanism. Some organic pollutants like PAHs and phthalates were removed mainly through biodegradation or mineralization, and their dissipation percentages were higher than those of PCDD/Fs and PCBs. Nevertheless, some recalcitrant organic pollutants could be sequestrated in organic fractions of sludge mixtures, and their levels and ARG abundance even increased after sludge composting in some studies, posing potential risks for land application. This review demonstrated that microbial community and their corresponding degradation for organic pollutants were influenced by different pollutants, bulking agents, composting methods and processes. Further research perspectives on removing organic pollutants during sludge composting were highlighted.

8.
Materials (Basel) ; 14(4)2021 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33546222

RESUMO

In this study, large-sized Al-Zn-Mg-Cu alloy billets were prepared by direct chill casting imposed with annular electromagnetic stirring and intercooling; a process named uniform direct chill casting. The effects of uniform direct chill casting on grain size and the alloying element distribution of the billets were investigated and compared with those of the normal direct chill casting method. The results show that the microstructures were refined and the homogeneity of the alloying elements distribution was greatly improved by imposing the annular electromagnetic stirring and intercooling. In uniform direct chill casting, explosive nucleation can be triggered, originating from the mold wall and dendrite fragments for grain refinement. The effects of electromagnetic stirring on macrosegregation are discussed with consideration of the centrifugal force that drives the movement of melt from the central part towards the upper-periphery part, which could suppress the macrosegregation of alloying elements. The refined grain can reduce the permeability of the melt in the mushy zone that can restrain macrosegregation.

9.
Phys Chem Chem Phys ; 2021 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-33624649

RESUMO

2.25Cr1Mo0.25V is a state-of the-art alloy used in the fabrication of modern hydrogenation reactors. Compared to the conventional 2.25Cr1Mo steel, the 2.25Cr1Mo0.25V steel exhibits a better performance, in particular higher hydrogen damage resistance. Previous experimental studies indicate that carbides in steels may be responsible for the hydrogen-induced damage. To gain a better understanding of the mechanism of such damage, it is essential to study hydrogen uptake in metal carbides. In this study, Density Functional Theory (DFT) is used to investigate the stability of chromium, molybdenum and vanadium carbides (CrxCy, MoxCy and VxCy) in the 2.25Cr1Mo0.25V steel. The stability of their corresponding interstitial hydrides was also explored. The results showed that Cr7C3, Mo2C and V6C5 are the most stable carbides in their respective metal-carbon (Cr-C, Mo-C and V-C) binary systems. Specifically, V6C5 shows the strongest hydrogen absorption ability because of its strong V-H and C-H ionic bonds. On the other hand, V4C3, whose presence in the alloy was established in experimental studies, is predicted to be stable as well, along with V6C5. Our findings indicate that the hydrogen absorption ability of V4C3 is higher than that of V6C5. Additionally, the charge and chemical bonding analyses reveal that the stability of the metal carbide hydrides strongly depends on the electronegativity of the metal. Due to the high electronegativity of V, vanadium carbides form the strongest ionic bonds with hydrogen, compared to those of Mo and Cr. The results from this study suggest that the unique capacity of accommodating hydrogen in the vanadium carbides plays an important role in improved hydrogen damage resistance of the 2.25Cr1Mo0.25V alloy in hydrogenation reactors.

10.
Nat Plants ; 7(2): 129-136, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33594262

RESUMO

MicroRNA168 (miR168) is a key miRNA that targets Argonaute1 (AGO1), a major component of the RNA-induced silencing complex1,2. Previously, we reported that miR168 expression was responsive to infection by Magnaporthe oryzae, the causal agent of rice blast disease3. However, how miR168 regulates immunity to rice blast and whether it affects rice development remains unclear. Here, we report our discovery that the suppression of miR168 by a target mimic (MIM168) not only improves grain yield and shortens flowering time in rice but also enhances immunity to M. oryzae. These results were validated through repeated tests in rice fields in the absence and presence of rice blast pressure. We found that the miR168-AGO1 module regulates miR535 to improve yield by increasing panicle number, miR164 to reduce flowering time, and miR1320 and miR164 to enhance immunity. Our discovery demonstrates that changes in a single miRNA enhance the expression of multiple agronomically important traits.

11.
Int Microbiol ; 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33604753

RESUMO

PURPOSE: In the present study, we characterized the microbiomes of acute leukemia (AL) patients who achieved complete remission following remission induction chemotherapy (RIC) as outpatients, but who did not receive antimicrobials to treat or prevent febrile neutropenia. METHODS: Saliva and stool samples from 9 patients with acute myeloid leukemia, 11 patients with acute lymphoblastic leukemia, and 5 healthy controls were subjected to 16S ribosomal RNA sequencing at baseline and at 3 months following RIC. Only patients who achieved remission at 3 months post-treatment were included. We excluded anyone who used antimicrobials within 2 months of enrollment or at any time during the study period. RESULTS: At baseline, the relative abundances of species of Prevotella maculosa (P=0.001), Megasphaera micronuciformis (P=0.014), Roseburia inulinivorans (P=0.021), and Bacteroides uniformis (P=0.004) in saliva and Prevotella copri (P=0.002) in the stools of controls were significantly higher than in AL patients. Following RIC, the relative abundances of Eubacterium sp. oral clone DO008 (P=0.012), Leptotrichia sp. oral clone IK040 (P=0.002), Oribacterium sp. oral taxon 108 (P=0.029), Megasphaera micronuciformis (P=0.016), TM7 phylum sp. oral clone DR034 (P<0.001), Roseburia inulinivorans (P=0.034), Actinomyces odontolyticus (P=0.014), Leptotrichia buccalis (P=0.005), and Prevotella melaninogenica (P=0.046) in saliva and Lactobacillus fermentum (P=0.046), Coprococcus catus (P=0.050), butyrate-producing bacterium SS3/4 (P=0.013), and Bacteroides coprocola (P=0.027) in the stools of AL patients were significantly greater than in controls. CONCLUSION: Following RIC, several taxa are changed in stool and salvia samples of AL patients. Our results warrant future large-scale multicenter studies to examine whether the microbiota might have an effect on clinical outcomes of AL patients.

12.
J Mater Chem B ; 9(4): 1096-1106, 2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33427278

RESUMO

Instead of directly stimulating osteogenesis, endowing an implant surface with a favourable osteoimmunomodulatory (OIM) function has emerged as a new effective strategy to enhance osteointegration. Though metal-phenolic coatings have demonstrated to possess an immunomodulatory function, their potential application in manipulating an osteoimmune response has not been well explored. Herein, in order to develop a simple, rapid and universal coating method to impart excellent OIM to hard tissue implants, tannic acid (TA) and Mg2+ were selected to form a coating on Ti plate based on metal-phenolic chemistry. Besides its virtues of simplicity, ultrafastness, low-cost, and versatility, another merit for the coating method is that it can easily combine the unique functions of metal ions and phenolic ligands. The chelated Mg2+ can not only activate macrophage polarization towards the anti-inflammatory phenotype but also directly stimulate the osteogenic differentiation of bone marrow-derived stem cells (BMSCs). TA motifs rendered the coating with an excellent reactive oxygen species (ROS) scavenging capacity. TA and Mg2+ showed synergistic effects on regulating macrophage biological behaviour, suppressing its polarization towards the M1 phenotype, and promoting its polarization towards the M2 phenotype. In vivo histological analysis also demonstrated that the TA/Mg2+ coating could effectively inhibit the host response. Finally, the formed osteoimmune environment obviously enhanced the osteogenic differentiation of BMSCs. The above results demonstrated that the designed TA/Mg2+ coating not only possessed the function of directly stimulating osteogenesis but also the function of manipulating OIM to a desired one. Hence, it has great potential to be applied on advanced hard tissue implants to enhance osteointegration.

13.
Artigo em Inglês | MEDLINE | ID: mdl-33438562

RESUMO

BACKGROUND: Alantolactone (AL) is a natural compound extracted from the roots of Inula Helenium L, which exerts an antitumor effect in a variety of cancer cell lines; however, its effect on esophageal cancer, a common malignancy with poor prognosis, remains unclear. Therefore, we aim to evaluate the effect of AL on esophageal cancer and to explore its underlying mechanism. OBJECTIVE: This study aims to determine whether AL has an anti-cancer effect on esophageal cancer cells and to explore its underlying mechanism. METHODS: The effect of AL on the proliferation and apoptosis of esophageal cancer cells was detected by MTT assay, colony formation assay, crystal violet assay, flow cytometry and hoechst apoptosis staining. The wound healing and Transwell invasion assay were performed to examine the effect of AL on the migration and invasion of esophageal cancer cells. Luciferase reporter system and Western blot were used to study the anti-tumor mechanism of AL on esophageal cancer cells. The subcutaneous murine xenograft model was employed to verify the effects of AL on esophageal cancer cells . RESULTS: MTT assay, colony formation assay and crystal violet assay found that AL inhibited the growth of esophageal cancer cells. Hoechst staining and flow cytometry analysis showed that AL induced apoptosis in esophageal cancer through mitochondrial pathway. Transwell assay and wound healing assays showed that AL inhibited the metastasis and invasion of esophageal cancer cells. Wnt/ ßcatenin signaling may contribute to the mechanism of the inhibition. The anti-tumor effect of AL on esophageal cancer cells was validated on murine xenograft model. CONCLUSION: Our data indicate that AL inhibits proliferation, migration, and invasion of esophageal cancer cells, and promotes apoptosis of esophageal cancer cells through the Wnt/ß-catenin signaling pathway.

15.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 52(1): 149-152, 2021 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-33474905

RESUMO

Objective: This study investigated the clinicopathological traits and ultrasound features of female reproductive system extraosseous Ewing's sarcoma (EES) and explored the diagnostic value of ultrasonography for this condition. Methods: Cases of female pelvic EES diagnosed and treated at our hospital between June 2009 and June 2019 were included in this study. Pathology data and ultrasound manifestations were assessed retrospectively to summarize the clinical traits and ultrasound features of female reproductive system EES. Based on the results, recommendations for the ultrasonography-based diagnosis of this disease were proposed. Results: During the 10-year study period, 13 female patients were diagnosed with EES in the pelvic cavity based on the results of postoperative pathology tests. The age of the patients ranged from 8 mouth to 40 years, and no patients demonstrated specific clinical symptoms. However, an examination of tumor biomarkers revealed that certain patients had elevated levels of CA125. In the 13 patients, 19 lesions were identified, including 16 that involved the reproductive system. The primary ultrasound manifestation was uneven, low-echo solidity or cystic solidity, exhibiting large size, irregular shape, and unclear boundary. A few patients had concurrent ascites. Although some lesions lacked blood supply, the blood supply of most lesions was medium to abundant, and the blood flow was mostly characterized by low resistant. Almost none of the lesions were definitively diagnosed preoperatively. Conclusions: Preoperative definitive diagnosis of EES in the female reproductive system remains a great clinical challenge. Although certain clinical traits and ultrasound features are associated with this disease, and color Doppler ultrasonography might provide vital information indicating the presence of EES, the final diagnosis still depends on the pathological test results of the patients.


Assuntos
Sarcoma de Ewing , Criança , Feminino , Genitália Feminina , Humanos , Estudos Retrospectivos , Sarcoma de Ewing/diagnóstico por imagem , Ultrassonografia , Ultrassonografia Doppler em Cores
16.
Ren Fail ; 43(1): 206-215, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33478333

RESUMO

BACKGROUND: Acute kidney injury (AKI) is a common complication in traumatic brain injury (TBI) patients and is associated with unfavorable outcome of these patients. We designed this study to explore the value of serum cystatin C, an indicator of renal function, on predicting AKI after suffering TBI. METHODS: Patients confirmed with TBI and hospitalized in the West China Hospital of Sichuan University between January 2015 and December 2019 were included. Patients were divided into two groups according to occurrence of AKI. Univariate and multivariate logistic regression analyses were sequentially utilized to find risk factors of AKI in included TBI patients. Nomogram composed of discovered risk factors for predicting AKI was constructed. Receiver operating characteristics (ROC) curves were drawn and area under the ROC curve (AUC) were calculated to evaluate the predictive value of cystatin C alone and the constructed nomogram. RESULTS: Among 234 included TBI patients, 55 were divided into AKI group. AKI group had shorter length of stay (p < 0.001) and higher in-hospital mortality (p < 0.001). Multivariate logistic regression analysis showed absolute lymphocyte count (p = 0.034), serum creatinine (p < 0.001), serum cystatin C (p = 0.017) and transfusion of red blood cell (p = 0.005) were independently associated with development of AKI after TBI. While hypertonic saline use was not associated with the development of AKI (p = 0.067). The AUC of single cystatin C and predictive nomogram were 0.804 and 0.925, respectively. CONCLUSION: Higher serum cystatin C is associated with development of AKI in TBI patients. Predictive nomogram incorporating cystatin C is beneficial for physicians to evaluate possibilities of AKI and consequently adjust treatment strategies to avoid occurrence of AKI.

17.
Medicine (Baltimore) ; 100(2): e23717, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33466124

RESUMO

ABSTRACT: Renal cell carcinoma (RCC) is infrequent in the pediatric population. In addition, till date, only a few reports have summarized the characteristics of pediatric RCC and differences between pediatric and adult RCC. Therefore, the current study aimed to investigate the clinical characteristics of RCC in children and adolescents, and identify the differences between children and adolescent patients and adult patients through literature retrieval.The data of 13 pediatric patients diagnosed with RCC at the Children's Hospital of Zhejiang University School of Medicine between 2005 and 2019 were retrospectively analyzed.Three patients were aged <5 years, 2 were aged 6 to 10 years, and 8 were aged 11 to 18 years. Among the 13 patients, common clinical manifestations included abdominal pain in 5 patients, gross hematuria in 4, and an abdominal mass in 1, while the other 3 patients were incidentally detected after an abdominal contusion. The pathological types were microphthalmia family translocation RCC in 9 patients, clear-cell RCC in 2, papillary RCC in 1, and unclassified in 1. All the children underwent radical nephrectomy, including 2 patients with advanced disease who underwent preoperative transcatheter arterial chemoembolization. The mean follow-up time was 58.6 months. Two patients died after 4 and 17 months of follow-up, respectively.In conclusion, microphthalmia family translocation renal cell carcinoma is the predominant type of pediatric RCC associated with advanced tumor stage. The early diagnosis and treatment of pediatric patients is important for improving prognosis. Nevertheless, future studies are urgently needed to determine the treatment for pediatric advanced RCC to increase the survival rate.


Assuntos
Carcinoma de Células Renais/patologia , Neoplasias Renais/patologia , Adolescente , Carcinoma de Células Renais/cirurgia , Criança , Pré-Escolar , Feminino , Humanos , Neoplasias Renais/cirurgia , Masculino , Nefrectomia/métodos , Estudos Retrospectivos
18.
J Ethnopharmacol ; 265: 113115, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32891812

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Ancient Egyptian texts only offer glimpses into their conceptual understandings of the inner-body and illness manifestation. Explanations of how prescribed materia medica were believed to work are rare and obscure, often resulting in modern approximations for ancient terminology such as 'ra-ib'-an ancient Egyptian classification predominantly translated as 'stomach'-leading to misunderstandings of historical texts, and therefore their use of pharmacology. AIM OF THE STUDY: To investigate the ra-ib and the explanatory models of illness from the Egyptian perspective, and to explore the link between these and the prescribed selection of materia medica. To then compare the conceptual mechanics of these treatment strategies with those of another non-Western tradition-namely Traditional Chinese Medicine (TCM)-to provide further insight into potential conceptual frameworks. MATERIALS AND METHODS: We conducted a case study of a unit of Ancient Egyptian texts focusing on the ra-ib. Totalling 34 prescriptions, the first stage lexicographically analysed the texts using cognitive linguistic and translation theories to produce our new understanding. This enabled our comparison of the mechanics of materia medica usage within these texts with those found in TCM outlined by the Pharmacopoeia of the Peoples Republic of Pharmacopeia of the People's Republic of China 2015 for the relevant ingredients. RESULTS: the study demonstrated that-rather than denoting the organ 'stomach'-ra-ib instead constitutes a system running from the mouth, downward to the anus. This is best translated as 'inner thoroughfare', and changes the way in which we attempt to understand potential motivations in the selection of ingredients. By exploring common themes in the use of eleven securely translated ingredients from the Egyptian corpus and the Pharmacopoeia of the People's Republic of China-representing a modern traditional system which understands the body via a series of interconnected systems-we were able to highlight certain themes which might be 'universal' to system-based traditions; this provided new insights into the Egyptian motivations for treatment selection. CONCLUSIONS: Having gained the ancient view of the body and illness, cultural comparisons are important for providing further potential insights and clarifications of a discontinued historical healing tradition. The new understanding of the ra-ib from our study greatly changes the way in which we understand the dynamics of Egyptian ethnopharmacological source material from this period.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Materia Medica/história , Medicina Tradicional Chinesa/métodos , Antigo Egito , Etnofarmacologia , História Antiga , Humanos , Materia Medica/farmacologia , Farmacopeias como Assunto
19.
Medicine (Baltimore) ; 99(49): e23163, 2020 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-33285691

RESUMO

BACKGROUND: MicroRNA 221 has been found to be a good marker for several cancers. Some studies also focused on the relationship between microRNA 221 and glioma. However, the results are controversial. We aimed to systematically evaluate the prognostic role of microRNA 221 in glioma through performing a meta-analysis. METHODS: The articles which were included in our study were searched on the Web of Science, EMBASE, PubMed, Cochrane Library and China National Knowledge Infrastructure. The basic characteristics and relevant data were extracted. Hazard ratios (HRs) with 95% confidence intervals (CIs) were pooled to evaluate the prognostic role of microRNA 221 in glioma. RESULTS: Eight studies with 1069 patients were included. We systematically evaluated the role of microRNA 221 for overall survival (OS) and disease free survival (DFS) in glioma patients (HR for OS = 1.66, 95% CI, 1.34-2.04; HR for DFS = 1.14, 95% CI, 1.02-1.26). Subgroup analyses were performed according to the nation of the studies, the origin of the samples, the stage of the tumors, the cut-off value, and the method for detecting the microRNA 221. No significant publication bias was found (P = .133). CONCLUSION: In conclusion, high expression of microRNA 221 was related to poor prognosis of glioma. These findings may assist future exploration on microRNA 221 and help predict the prognosis of glioma. However, due to the significant heterogeneity of these studies, more studies are warranted.

20.
J Pharm Biomed Anal ; : 113800, 2020 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-33281000

RESUMO

In order to facilitate correlation calculation and matrix-based resolution in comprehensive two-dimensional gas chromatography - mass spectrometry (GC × GC-MS) data-set, an intelligent clustering of modulation peaks (ICMP) algorithm was developed in this paper. ICMP is start with the second -dimension (2D) peak restriction, then conducting the peak shape restriction in the first dimension (1D), finally end with the eigenvalues calculation against mass spectra in moving sub-windows. After this three-tier restriction, multi-component spectral correlative chromatography (MSCC) was applied in peak clustering result from a row-wise augmented "two-dimension (2D) slice" set. Then the component similarities and differences were distinguished rapidly/ accurately in chemical fingerprints from ChaiHu Shugan San and Cyperus rotundus. Faced with co-eluted phenomenon, matrix-based resolution was made in the representative sub-matrices that have been locked in ICMP procedure. From the example data shows that ICMP- multivariate curve resolution (MCR) can served as a good complement to (non) trilinear decomposition. To summarize, the GC × GC data-structure can be simplified to facilitate MSCC or MCR operation in fingerprints from herbal or biological samples.

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