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1.
Phys Rev Lett ; 124(16): 163201, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-32383919

RESUMO

We demonstrate the possibility of realizing Young's double-slit interference in a hydrogen atom via ab initio simulations. By exposing the hydrogen atom to a high-frequency intensive laser pulse, the bound state distorts into a dichotomic Kramers-Henneberger state whose photoelectron momentum distribution imprints a double-slit interference structure. The dichotomic hydrogen atom presents molecular peculiarities, such as charge-resonance enhanced ionization, electron spin flipping due to the non-Abelian Berry phase. In return, the photoelectron momentum distribution carrying the double-slit interference structure provides unambiguous evidence on the existence of Kramers-Henneberger states, and thus the adiabatic stabilization.

2.
Nanoscale ; 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32187246

RESUMO

Super-oscillatory lens (SOL) optical microscopy, behaving as a non-invasive and universal imaging technique, as well as being a simple post-processing procedure, may provide a potential application for sub-diffraction-limit fluorescence imaging. However, the low energy concentration, high-intensity sidelobes and micrometer-scale working distance of the reported planar SOLs impose unavoidable restrictions on the ground-state applications. Here, we demonstrate step-shaped SOLs based on the multiple-phase-modulated (MPM) method to improve the focusing efficiency. Two pivotal advantages are thus generated: (i) the fabrication complexity can be effectively reduced based on several conventional optical lithography steps; (ii) the focusing efficiency is much higher than that of the random MPM ones due to the efficient manipulation of the wavefronts, bringing about a stronger light concentration to the focal spot. Additionally, the ratio of the sidelobe intensity is flexibly tuned to meet the customized requirements, and a 2 mm-working-distance MPM SOL with the sidelobe intensity highly suppressed is finally exploited. For the first time, as far as we know, a SOL-based fluorescence microscopy without the pinhole filter to map the horizontal morphology of the dispersive fluorescent particles is established. Compared with the results achieved by the conventional wide-field microscopy, the sample details beating the diffraction limit can be reconstructed by simple imaging fusion. This research demonstrates the promising applications of SOLs for low-cost, simplified and highly customized sub-diffraction-limit fluorescence imaging systems free from photobleaching and an extremely short working distance.

3.
Pain ; 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32168198

RESUMO

Some forms of chronic pain are thought to be driven and maintained by nociceptive input, which can drive plasticity within nociceptive pathways. We have previously identified abnormalities along the entire nociceptive pathway in chronic myalgic temporomandibular disorders (mTMD), including the trigeminal nerves, brainstem pathways, and in the thalamus and somatosensory cortex. These data suggest that there is a peripheral nociceptive drive in mTMD, but the source of this nociceptive activity remains unknown. Here, our aim was to determine whether structural abnormalities exist in the muscles of mastication of patients with chronic mTMD. Specifically, we tested whether the volume of the temporalis muscle and its tendon-aponeurosis complex (TAC, a structure that dissipates forces in a muscle) in mTMD patients differ compared to age- and sex-matched controls. To do so, we segmented these structures on T1-weighted structural magnetic resonance images. We found that muscle volumes in mTMD were not different to controls. However, the mTMD group had significantly smaller volumes of the bilateral temporalis TAC, and thus a smaller TAC-to-muscle volume ratio. These findings were consistent across 2 independent cohorts of 17 mTMD patients, compared to 17 age- and sex-matched controls. We propose a model where reduced TAC-to-muscle ratio could result in a predisposition to muscle tissue injury. In sum, abnormalities of the temporalis muscles in mTMD supports our hypothesis that chronic mTMD pathophysiology may be related to peripheral nociceptive barrage originating from the muscles of mastication.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32101407

RESUMO

Electrodeposition of conducting polymer-carbon composites from an electrolyte precursor solution is a facile one-step approach to fabricate device-ready electrodes for energy storage. A key challenge in this approach is the dispersion of the carbon nanomaterials with the aqueous precursor solution with previous approaches either heavily oxidizing the carbon nanomaterials or using high concentrations of anionic surfactants as dopants. However, the former reduces the electrical conductivity of carbon, while the latter reduces the ionic mobility of the conducting polymer due to the large anion size. Herein, for the first time we present a quaternary electrolyte formulation for the fabrication of pristine carbon and polypyrrole (PPy) composites that does not sacrifice either electron or ion mobility. The electrolyte uses lithium perchlorate (20 mM) as a supporting electrolyte and dopant, sodium dodecylbenzenesulfonate at a very low concentration (1.43 mM) as a surfactant, together with pristine carbon nanomaterials and pyrrole monomers. The order of magnitude difference between the concentration of the dopant and surfactant ion allows the as-deposited PPy to be doped predominantly by small-sized and mobile perchlorate anions. Composites of PPy with carbon black, carbon nanotubes, and electrochemical exfoliated graphene (EEG) have been successfully prepared using this new quaternary electrolyte. The as-fabricated PPy/EEG composite electrodes showed a specific capacitance of 348.8 F g-1 with a high rate capability (190.7 F g-1 at 71 A g-1). Supercapacitor devices based on the PPy/EEG composite electrodes exhibit a high rate behavior up to 500 mV s-1 and a long cycle life of 5000 cycles.

5.
Mol Cell Biochem ; 465(1-2): 187-197, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894528

RESUMO

Polycystic ovary syndrome (PCOS) is a hormonal disorder common among women of reproductive age. Although much is understood concerning the pathology of PCOS, further investigation into the influence of microribonucleic acids (miRNAs) on the proliferation of ovarian granulosa cells (GCs) is needed. This study investigated the role of specific miRNAs in ovarian dysfunction of PCOS and its effect on the proliferation of GCs. Initially, miRNA profiling was performed on the ovarian cortexes of 15 rats in which PCOS had been induced and 15 rats without PCOS (non-PCOS). This mechanical study was performed on ovarian GCs extracted from human chorionic gonadotrophin (hCG)-induced rats. Insulin was used to treat GCs to establish the PCOS cell model. Increased Equus caballus mir-9119 expression was observed and confirmed in the insulin-induced model of PCOS in GCs (GC-PCOS) as well as in the hCG-induced rats when compared to non-PCOS rats and cells. Observation and confirmation were carried out through both miRNA array and quantitative PCR. In contrast, downregulation of the nuclear factor kappa B (NFκB) p65 was observed in the PCOS cell model. Additionally, annexin V, FITC, and propidium iodide flow cytometry showed overexpression of miR-9119-induced apoptosis. In this study, we revealed that miR-9119 inhibition regulates p65 expression levels in insulin-treated GCs by binding to the 3'-untranslated of p65. Additionally, regulation of p65 expression was positively correlated with the expression of the double-stranded RNA endoribonuclease DICER. Moreover, RNA silencing/overexpression of p65 affected the functional role of miR-9119. In conclusion, GCs of PCOS, the expression of miR-9119, and targeted NFκB/p65-DICER axis are upregulated in order to maintain cell viability and prevent apoptosis, thereby promoting Anti-Müllerian hormone production in GCs. This study may provide a new understanding of the mechanism of GC dysfunction.


Assuntos
Regulação Enzimológica da Expressão Gênica , Células da Granulosa/metabolismo , MicroRNAs/metabolismo , Síndrome do Ovário Policístico/metabolismo , Ribonuclease III/biossíntese , Animais , Hormônio Antimülleriano/metabolismo , Sobrevivência Celular , Feminino , Células da Granulosa/patologia , Cavalos , Humanos , Síndrome do Ovário Policístico/induzido quimicamente , Síndrome do Ovário Policístico/patologia , Ratos , Fator de Transcrição RelA/metabolismo
6.
Stem Cells Transl Med ; 9(4): 499-517, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31943851

RESUMO

Mesenchymal stem cells (MSCs) have been used in clinical studies to treat neurological diseases and damage. However, implanted MSCs do not achieve their regenerative effects by differentiating into and replacing neural cells. Instead, MSC secretome components mediate the regenerative effects of MSCs. MSC-derived extracellular vesicles (EVs)/exosomes carry cargo responsible for rescuing brain damage. We previously showed that EP4 antagonist-induced MSC EVs/exosomes have enhanced regenerative potential to rescue hippocampal damage, compared with EVs/exosomes from untreated MSCs. Here we show that EP4 antagonist-induced MSC EVs/exosomes promote neurosphere formation in vitro and increase neurogenesis and neuritogenesis in damaged hippocampi; basal MSC EVs/exosomes do not contribute to these regenerative effects. 2',3'-Cyclic nucleotide 3'-phosphodiesterase (CNP) levels in EP4 antagonist-induced MSC EVs/exosomes are 20-fold higher than CNP levels in basal MSC EVs/exosomes. Decreasing elevated exosomal CNP levels in EP4 antagonist-induced MSC EVs/exosomes reduced the efficacy of these EVs/exosomes in promoting ß3-tubulin polymerization and in converting toxic 2',3'-cAMP into neuroprotective adenosine. CNP-depleted EP4 antagonist-induced MSC EVs/exosomes lost the ability to promote neurogenesis and neuritogenesis in damaged hippocampi. Systemic administration of EV/exosomes from EP4 -antagonist derived MSC EVs/exosomes repaired cognition, learning, and memory deficiencies in mice caused by hippocampal damage. In contrast, CNP-depleted EP4 antagonist-induced MSC EVs/exosomes failed to repair this damage. Exosomal CNP contributes to the ability of EP4 antagonist-elicited MSC EVs/exosomes to promote neurogenesis and neuritogenesis in damaged hippocampi and recovery of cognition, memory, and learning. This experimental approach should be generally applicable to identifying the role of EV/exosomal components in eliciting a variety of biological responses.

7.
Int Immunopharmacol ; 80: 106196, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31978803

RESUMO

Sepsis-induced liver injury is very common in intensive care units. Here, we investigated the effects of 6-gingerol on sepsis-induced liver injury and the role of the Nrf2 pathway in this process. 6-Gingerol is the principal ingredient of ginger that exerts anti-inflammatory and antioxidant effects. Using cecal ligation and puncture (CLP) to induce polymicrobial sepsis and related liver injury, we found that mice pre-treated with 6-Gingerol showed less incidences of severe liver inflammation and death than untreated CLP groups. 6-Gingerol administration also inhibited the expression of pyroptosis-related proteins, including NOD-like receptor protein 3 (NLRP3), IL-1ß, and caspase-1. Consistent with these findings, 6-gingerol reduced the effects of pyroptosis induced by lipopolysaccharide (LPS) and adenosine 5'-triphosphate (ATP) in RAW 264.7 cells, as evidenced by IL-1ß and caspase-1 protein levels in the supernatant and propidium iodide (PI) staining. 6-Gingerol was shown to activate the Nrf2 pathway in vivo and in vitro. Notably, Nrf2 siRNA transfection nullified the inhibitory effects of 6-gingerol on pyroptosis in vitro. In summary, these findings suggested that 6-gingerol alleviated sepsis-induced liver injury by inhibiting pyroptosis through the Nrf2 pathway.

8.
Chin J Integr Med ; 2019 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-31872369

RESUMO

OBJECTIVE: To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest (EBN) in ovariectomized rats. METHODS: Forty-two female Sprage-Dawley rats were randomized into 7 groups (6 in each group). The ovariectomized (OVX) and OVX + 6%, 3%, and 1.5% EBN and OVX +estrogen groups were given standard rat chow alone, standard rat chow +6%, 3%, and 1.5% EBN, or standard rat chow +estrogen therapy (0.2mg/kg per day), respectively. The sham-operation group was surgically opened without removing the ovaries. The control group did not have any surgical intervention. After 12 weeks of intervention, blood samples were taken for serum estrogen, osteocalcin, and osteoprotegerin, as well as the measurement of magnesium, calcium abd zinc concentrations. While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique, then collected for histology and estrogen receptor (ER) immunohistochemistry. RESULTS: Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain, while estrogen and EBN supplementation attenuated these changes. Ovariectomy also reduced bone ER expression and density, and the production of osteopcalcin and osteorotegerin, which are important pro-osteoplastic hormones that promote bone mineraliztion and density. Conversely, estrogen and EBN increased serum estrogen levels leading to increased bone ER expression, pro-osteoplastic hormone production and bone density (all P<0.05). CONCLUSION: EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.

9.
J Immunol Res ; 2019: 7684352, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31781682

RESUMO

Background: We have reported previously the insufficient absolute number or functional defects of regulatory T cells (Tregs) in patients with rheumatoid arthritis (RA), challenging conventional unspecific immunosuppressive therapy. Sirolimus, a mTOR inhibitor, is reported to allow growth of functional Tregs; here, we investigated the efficacy of low-dose sirolimus combined with conventional immunosuppressants (sirolimus immunoregulation therapy) for RA treatment with lower side effects and better tolerance. Methods: In this nonblinded and parallel-group trial, we randomly assigned 62 patients to receive conventional glucocorticoids and immunosuppressants with or without sirolimus at a dosage of 0.5 mg on alternate days for 24 weeks in a 2 : 1 ratio. The demographic features, clinical manifestations, and laboratory indicators including peripheral blood lymphocyte subgroups and CD4+T subsets were compared before and after the treatment. Results: Finally, 37 patients in the sirolimus group and 18 in the conventional treated group completed the 6-month study. By 24 weeks, the patients with sirolimus experienced significant reduction in disease activity indicators including DAS28, ESR, and the number of tender joints and swollen joints (p < 0.001). Notably, they had a higher level of Tregs as compared with those with conventional therapy alone (p < 0.05), indicating that sirolimus could partly restore the reduced Tregs. Concomitantly, their usage of immunosuppressants for controlling disease activity was decreased as compared with the conventional group with no difference in blood routine, and liver and renal functions both before and after the treatment of sirolimus and between the two groups (p > 0.05). Conclusions: Low-dose sirolimus immunoregulatory therapy selectively upregulated Tregs and partly replaced the usage of immunosuppressants to control disease activity without overtreatment and evaluable side effect. Further study is required using a large sample of RA patients treated with sirolimus for a longer period. This trial is registered at the Chinese Clinical Trial Registry (http://www.chictr.org.cn/showproj.aspx?proj=17245).

10.
Artigo em Inglês | MEDLINE | ID: mdl-31757069

RESUMO

The starting point of organizational innovation is employees' creative thinking and innovation behaviors at work. In addition to personality and innovation willingness, innovation behavior depends on the level of support available in an organizational environment. The data used in this study were collected from 74 R&D teams (418 employee participants) in technology companies in Taiwan, and a multi-level analysis was conducted to investigate the relationships among job stressors, creative self-efficacy, and employees' sustained innovation behavior, as well as the role of the organizational innovation climate between creative self-efficacy and employees' innovation behavior. The research findings revealed significant positive relationships between challenge stressors and employees' sustained innovation behavior, as well as significant negative relationships between hindrance stressors and employees' sustained innovation behavior, mediation effects of creative self-efficacy on job stressors and employees' sustained innovation behavior, and moderation effects of the organizational innovation climate on employees' creative self-efficacy and sustained innovation behavior. An enterprise could place some working-related stress on employees and create a rich internal innovative climate to induce innovation behavior in its members.

11.
Nanoscale ; 11(44): 21386-21393, 2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31674616

RESUMO

Perovskite cesium lead halide (CsPbBr3) has attracted considerable attention due to its excellent optoelectronic properties and superior stability against moisture, oxygen, light, and heat. In this work, the micro-environment controlled chemical vapor deposition (CVD) method has been adopted to synthesize high-quality single-crystalline CsPbBr3 microstructures, including microwires, microplates and triangular pyramids. Moreover, the structure-activity relationship between the material microstructures and the device properties is illustrated. The results show that photodetectors based on a single horizontal CsPbBr3 microwire exhibit a high responsivity (312.2 A W-1) and a fast response time of 5.8 ms. Photodetectors based on a single CsPbBr3 microplate exhibit a responsivity of 1.74 A W-1 and a response of 10 ms. These results indicate that the CsPbBr3 microwire photodetector is characterized by a higher photodetector performance when compared to the microplate due to its excellent crystallization quality and the Fabry-Pérot cavity effect in the microwire. Furthermore, the flexible CsPbBr3 microwire photodetector was demonstrated on a mica substrate. The results show that the photocurrent can be maintained at 90% after 3000 cycles at a bending radius of 2.5 mm. This work demonstrates the structure-activity photodetector performance, which is essential to develop a full understanding about high-performance optoelectronic devices based on all-inorganic lead halide perovskite materials.

12.
Phys Rev Lett ; 123(17): 174801, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31702272

RESUMO

The generation of ultrarelativistic polarized positrons during the interaction of an ultrarelativistic electron beam with a counterpropagating two-color petawatt laser pulse is investigated theoretically. Our Monte Carlo simulation, based on a semiclassical model, incorporates photon emissions and pair productions, using spin-resolved quantum probabilities in the local constant field approximation, and describes the polarization of electrons and positrons for the pair production and photon emission processes, as well as the classical spin precession in between. The main reason for the polarization is shown to be the spin asymmetry of the pair production process in strong external fields, combined with the asymmetry of the two-color laser field. Employing a feasible scenario, we show that highly polarized positron beams, with a polarization degree of ζ≈60%, can be produced in a femtosecond timescale, with a small angular divergence, ∼74 mrad, and high density, ∼10^{14} cm^{-3}. The laser-driven polarized positron source raises hope for providing an alternative for high-energy physics studies.

13.
Epigenomics ; 11(14): 1601-1612, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31693422

RESUMO

Aim: To identify epigenetically regulated network of genes in peripheral blood mononuclear cells significant for rheumatoid arthritis (RA). Methods: Differentially expressed genes (DEGs) and their associated differentially expressed miRNAs and differentially methylated positions (DMPs) were identified. Causal inference test (CIT) identified the causal regulation chains. The analyses, for example, weighted gene co-expression network (WGCNA), protein-protein interaction and functional enrichment, evaluated interaction patterns among the DEGs and the associated epigenetic factors. Results: A total of 181 DEGs were identified. The DEGs were significantly regulated by DMPs and/or differentially expressed miRNAs. Causal inference test analyses identified 18 causal chains of DMP-DEG-RA and 16 intermediate DEGs enriched in 'protein kinase inhibitor activity'. BTN2A1 was co-expressed with other 9 intermediate genes and 11 known RA-associated genes and played a pivotal role in the co-expression network. Conclusion: Epigenetically regulated network of genes in peripheral blood mononuclear cells (PBMC) contributed to RA. The causal DMPs and key intermediate genes may serve as potential biomarkers for RA.

14.
J Comput Biol ; 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31573330

RESUMO

This study aimed to identify possible therapeutic targets involved in sleep deprivation (SD) risks. GSE77393 data set was acquired from Gene Expression Omnibus database. Functional analysis and protein-protein interaction (PPI) analysis were used to extract the differentially expressed genes (DEGs) between two SD samples and control samples. Moreover, submodule network with the same function was further extracted and the functional enrichment analysis of corresponding genes was carried out. Afterward, the transcriptional regulation analysis and drug-gene interaction were also carried out to identify the essential genes associated with SD susceptibility. Totally, 121 DEGs, including 90 consistently upregulated DEGs and 31 downregulated DEGs, were screened and the results of functional analysis indicated that upregulated genes were related to learning or memory and response to drug, whereas downregulated DEGs were mainly responsible for response to UV and cell differentiation. Moreover, PPI network and submodule analysis revealed that many key genes (FOS and BDNF) were hub genes and the KEGG enrichment analysis found that these genes such as FOS and BDNF were considerably enriched in pathways such as MAPK signaling pathway, HTLV-I infection, and Hepatitis B. In addition, two genes (FOS and BDNF) with a higher degree were found to be key regulators and play important roles in the transcriptional regulator network and drug-gene interactions, suggesting that these two genes were associated with SD development. FOS and BDNF might be served as the potential targets for SD treatment.

16.
ACS Appl Mater Interfaces ; 11(35): 32225-32234, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31390171

RESUMO

Conductive inks for the future printed electronics should have the following merits: high conductivity, flexibility, low cost, and compatibility with wide range of substrates. However, the state-of-the-art conductive inks based on metal nanoparticles are high in cost and poor in flexibility. Herein, we reported a highly conductive, low cost, and super flexible ink based on graphene nanoplatelets. The graphene ink has been screen-printed on plastic and paper substrates. Combined with postprinting treatments including thermal annealing and compression rolling, the printed graphene pattern shows a high conductivity of 8.81 × 104 S m-1 and good flexibility without significant conductivity loss after 1000 bending cycles. We further demonstrate that the printed highly conductive graphene patterns can act as current collectors for supercapacitors. The supercapacitor with the printed graphene pattern as the current collector and printed activated carbon as the active material shows a good rate capability of up to 200 mV s-1. This work potentially provides a promising route toward the large-scale fabrication of low cost yet flexible printed electronic devices.

17.
Infect Genet Evol ; 75: 104008, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31437557

RESUMO

Haemaphysalis flava Neumann, 1897 (H. flava) is of public health significance due to its capability of transmitting several pathogens such as Rickettsia, Ehrlichia, Bartonella and Francisella tularensis. However, lack of complete genome, transcriptome and proteome information limits the understanding of the biology of H. flava. Here, the total RNA of H. flava was collected separately at larvae and nymph stages and analyzed with high-throughput RNA sequencing technology. The obtained data were assembled and annotated based on the near origin species in the Nr database. The functions of the unigenes were annotated and classified by seven databases, including Nr, Nt, Pfam, KOG, Swiss-Prot, GO and KEGG. A total of 61,850,967 and 79,579,368 clean reads were obtained with a data bulk of 9.28 G and 11.94 G in larvae and nymph stages, respectively. The number of unigenes was 440,896, with 48.6% of them being matched to the Nr database and 51.4% remaining unknown. Additionally, 1,776,404 SNPs were identified in the unigenes. Differential analysis revealed 80 differentially expressed genes (DEGs), including 56 up-regulated genes and 24 down-regulated genes in the nymph versus larvae. qPCR confirmed 4 of the 56 up-regulated genes and 4 of the down-regulated genes. KEGG analysis of the DEGs showed that aldehyde dehydrogenase and sorbitol dehydrogenase, two up-regulated unigenes in nymph versus larvae, were both matched to the top three enriched pathways: "chloroalkane and chloroalkene degradation", "fatty acid degradation" and "glycolysis and gluconeogenesis". This is the first report on the whole transcriptome of H. flava at larvae and nymph stages. This study contributes to the understanding of H. flava at the gene expression level in different developmental stages and provides a theoretical basis for the development of vaccines against H. flava.

18.
Oncol Lett ; 18(2): 1949-1960, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31423265

RESUMO

The objectives of the present study were to identify the aberrant expression of microRNA (miRNA) in colorectal carcinoma (CRC) tissues from published miRNA profiling studies and to investigate the effects of the identified miRNA inhibitor and mimic miR-96-5p on CRC cell migration and invasion. The altered expression of the regulators of cytoskeleton mRNA in miR-96-5p inhibitor-transfected cells was determined. The miR-96-5p expression level in five CRC cell lines, HCT11, CaCo2, HT29, SW480 and SW620, and 26 archived paraffin-embedded CRC tissues were also investigated by reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR). Cell viability in response to the miR-96-5p inhibitor and mimic transfections was determined by an MTT assay. A Matrigel invasion assay was conducted to select the invasive subpopulation designated SW480-7, by using the parental cell line SW480. The effects of miR-96-5p mimic- or inhibitor-transfected SW480-7 cells on cell migration and invasion were evaluated using the Transwell and Matrigel assays, and the change in expression of the regulators of cytoskeleton mRNAs was identified by Cytoskeleton Regulators RT2-Profiler PCR array followed by validation with RT-qPCR. CRC tissues exhibited a significant increase in miR-96-5p expression, compared with their matched normal adjacent tissues, indicating an oncogenic role for miR-96-5p. The results demonstrated that the miR-96-5p inhibitor decreased the migration of SW480-7 cells, but had no effect on invasion. This may be due to the promotion of cell invasion by Matrigel, which counteracts the blockade of cell invasion by the miR-96-5p inhibitor. The miR-96-5p mimic enhanced SW480-7 cell migration and invasion, as expected. It was determined that there was a >2.5 fold increase in the expression of genes involved in cytoskeleton regulation, myosin light chain kinase 2, pleckstrin homology like domain family B member 2, cyclin A1, IQ motif containing GTPase activating protein 2, Brain-specific angiogenesisinhibitor 1-associated protein 2 and microtubule-actin crosslinking factor 1, in miR-96-5p inhibitor-transfected cells, indicating that they are negative regulators of cell migration. In conclusion, the miR-96-5p inhibitor blocked cell migration but not invasion, and the latter may be due to the counteraction of Matrigel, which has been demonstrated to stimulate cell invasion.

19.
Analyst ; 144(18): 5324-5352, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31348475

RESUMO

Medicinal plants with complex matrices are endowed with a wide scope of biological activities. The separation, quantification, characterization and purification of bioactive components from herbal medicine extracts have always challenged analysts. Fortunately, the advancement of various emerging techniques has provided potent support for improving the method selectivity, sensitivity and run speeds in medicinal plant analyses. In recent years, the advent of new-generation supercritical fluid chromatography (SFC) instruments and a wide diversity of column chemistries, coupled with the intrinsic technical features of SFC, have made it an alternative and prominent analytical platform in the medicinal plant research area. This work aims to give a comprehensive overview of the fundamentals, technical advancement and investigating parameters of SFC in combination with three prevalent detectors. Moreover, the latest research progress of SFC applications in medicinal plant analyses is illuminated, with focus on herbal medicine-related SFC papers on the analytical and preparative scale that were published during the period of 2012 to December 2018. The most relevant applications were classified based on the constituents to be analysed. As for the respective research cases, analytical protocols and data processing strategies were provided, along with the indicated restrictions or superiority of the method; thus, the current status of SFC in medicinal plant analysis was presented.


Assuntos
Cromatografia com Fluido Supercrítico/métodos , Compostos Fitoquímicos/análise , Plantas Medicinais/química , Cromatografia com Fluido Supercrítico/instrumentação
20.
Sensors (Basel) ; 19(13)2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31266226

RESUMO

Electrocardiogram (ECG) signals are crucial for determining the health status of the human heart. A clean ECG signal is critical in analysis and diagnosis of heart diseases. However, ECG signals are often contaminated by motion artifact noise in the non-contact ECG monitoring systems. In this paper, an ECG motion artifact removal approach based on empirical wavelet transform (EWT) and wavelet thresholding (WT) is proposed. This method consists of five steps, namely, spectrum preprocessing, spectrum segmentation, EWT decomposition, wavelet threshold denoising, and EWT reconstruction. The proposed approach was used to process real ECG signals collected by the non-contact ECG monitoring equipment. The results of quantitative study and analysis indicate that this approach produces a better performance in terms of restorage of QRS complexes of the original ECG with reduced distortion, retaining useful information in ECG signals, and improvement of the signal to noise ratio (SNR) value of the signal. The output results of the practical ECG signal test show that motion artifact in the real recorded ECG is effectively filtered out. The proposed method is feasible for reducing motion artifacts from ECG signals, whether from simulation ECG signals or practical non-contact ECG monitoring systems.


Assuntos
Arritmias Cardíacas/diagnóstico , Eletrocardiografia/métodos , Monitorização Fisiológica , Processamento de Sinais Assistido por Computador , Algoritmos , Simulação por Computador , Humanos , Movimento (Física) , Análise de Ondaletas
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