Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 19 de 19
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi ; 54(12): 881-887, 2019 Dec 07.
Artigo em Chinês | MEDLINE | ID: mdl-31887812

RESUMO

Objective: To screen, diagnose and follow up the abnormal mutation in the gene screening of neonatal deafness. Methods: A total of 24161 newborns born in Zhuhai Maternal and Child Health Hospital from February 1, 2015 to January 31, 2008 were screened for hearing and deafness genes, and audiological screening, diagnosis and 1-3 years follow-up were carried out for the newborns with positive gene screening. Results: There were 991 cases of deafness gene mutation (533 males and 458 females), and the rate of abnormal mutation was 4.10%(991/24 161). Among them, 921 cases were single heterozygous mutation, 130 cases were failed in primary hearing screening, 11 cases were failed in secondary hearing screening, 8 cases were abnormal in audiological diagnosis finally. In these 8 cases, 3 were diagnosed as otitis media and passed audiological follow-up after cure, 2 cases of single ear sensorineural injury caused by high-risk factors, passed after close audiological follow-up, and the other 3 cases were closely audiological follow-up while none of them were successfully sequenced. All of them were moderate to severe sensorineural deafness, 1 case was heterozygous mutation at 3 loci of GJB2(c.235delC,c.408C>A,c.134G>A), 1 case was heterozygous mutation at 2 loci of GJB2(c.235delC, c.109G>A), and 1 case was single heterozygous mutation of GJB2(c.235delC). The remaining 913 cases who passed the primary screening, secondary screening or hearing diagnosis were followed up for 1 to 3 years. Three cases of multiple heterozygous mutation were found in gene screening(2 cases were SLC26A4 2168A>G, IVS7-2A>G, 1 case was GJB2 c.176_191del 16bp, c.299_300del AT), all of them passed both primary and secondary hearing screening. In these 3 cases, the final audiological diagnosis was moderate sensorineural deafness in both ears, with no improvement in the follow-up of 1-3 years. There were 9 monogenic homozygous mutations, 7 failed in primary hearing screening, 3 failed in secondary hearing screening and also failed in audiological diagnosis and 1-3 years' audiological follow-up, all of whom were GJB2 c.235 del C homozygous mutations, and one of whom had a definite family history of deafness. The remaining 6 cases of homozygous mutation diagnosed by primary screening, secondary screening or hearing diagnosis were GJB2 c109G>A homozygous mutation, and passed the 1-3 years' hearing follow-up. 58 children with mtDNA mutations, including 2 with 12S rRNA 1494C>T homozygous mutation, 47 with 1555A>G homozygous mutation, and 9 with 1555A>G heterozygous mutation, all passed the primary or secondary hearing screening, and were instructed to ban ototoxic drugs for the whole life, and passed the 1-3 years' hearing follow-up. Conclusions: The audiological follow-up of children with monogenic heterozygous mutations in deafness gene screening is generally normal. In case of abnormality, the influencing factors such as otitis media should be excluded at first. In case of unexplained moderate to severe sensorineural deafness, the whole-gene sequencing should be performed to find possible pathogenic factors. The children with homozygous mutation or compound heterozygous mutation in gene screening, most of whom show different degrees of hearing loss, should be followed up for a long time, and provide parents with scientific and reasonable genetic counseling according to the mutation genes and loci,. The hearing of drug-induced deafness gene carriers is normal after birth. Parents should be advised to strengthen prevention and follow-up is generally enough.


Assuntos
Análise Mutacional de DNA , Surdez , Perda Auditiva , Pré-Escolar , China , Conexina 26 , Conexinas , Feminino , Seguimentos , Testes Genéticos , Perda Auditiva/diagnóstico , Perda Auditiva/genética , Humanos , Lactente , Recém-Nascido , Masculino , Mutação , Triagem Neonatal
2.
Zhonghua Nei Ke Za Zhi ; 58(11): 826-828, 2019 Nov 01.
Artigo em Chinês | MEDLINE | ID: mdl-31665859

RESUMO

This study aims to explore the diagnostic value of specific immunoglobulin E (sIgE) and specific immunoglobulin G (sIgG) of Aspergillus fumigatus in the diagnosis of allergic broncho-pulmonary aspergillosis (ABPA) and severe asthma with fungal sensitization (SAFS). A total of 17 ABPA patients and 14 SAFS patients were enrolled. The levels of sIgG [2 294.00 (1 527.00, 14 170.00) U/ml vs. 972.60 (650.90, 1 792.00) U/ml] and sIgE [8.77 (1.64, 16.85) kU/L vs. 1.04 (0.70, 2.05) kU/L] in ABPA patients were significantly higher than those in SAFS patients (P<0.05). Aspergillus fumigatus sIgG was strongly correlated with Aspergillus fumigatus sIgE (r(s)=0.797, P<0.001) in ABPA patients. When combined with Aspergillus fumigatus sIgG (>1 000.00 U/mL) and Aspergillus fumigatus sIgE (>1.00 kU/L), the sensitivity was 82.3% and specificity 78.6% for the differential diagnosis of ABPA and SAFS. It demonstrates the diagnostic value of Aspergillus fumigatus sIgG and sIgE.


Assuntos
Aspergilose Broncopulmonar Alérgica/diagnóstico , Aspergillus fumigatus/isolamento & purificação , Asma/complicações , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Anticorpos Antifúngicos/imunologia , Aspergilose Broncopulmonar Alérgica/sangue , Aspergilose Broncopulmonar Alérgica/imunologia , Aspergilose Broncopulmonar Alérgica/microbiologia , Aspergillus fumigatus/imunologia , Asma/sangue , Asma/diagnóstico , Humanos , Imunoglobulina E/imunologia , Imunoglobulina G/imunologia , Índice de Gravidade de Doença
3.
Artigo em Chinês | MEDLINE | ID: mdl-30813698

RESUMO

Objective:To synchronously perform external auricle examination during neonatal hearing screening, follow up auricle deformity with neonatal disease screening system, and calculate the incidence of auricle deformity, self-healing rate, correction rate, incidence of complications and the relationship with hearing loss in Zhuhai area. Method:According to the diagnostic criteria of auricle deformity, the newborns in Zhuhai Maternal and Child Health Hospital were examined on the spot within 2 months. The deformity auricle was registered and uploaded into the newborn hearing screening system. The newborns were followed up by short message notification 7 days after birth, and then compared with the photo uploading system again. At 14 days, the ears of those who could not self-heal were went on non-invasive correction, and collect of relevant data for summary analysis. Result:Among the 1 073 newborns(2 146 ears), 26(37 ears) with malformed ears were treated with auricular pattern correction.The corrective rates of newborns less than 14 days, 14-30 days and 31-60 days were 95%, 90% and 87% respectively, and the incidence of complications were 50%, 58% and 69%, respectively. Conclusion:The incidence of auricular deformities in neonates is high. The earlier correction the better. The ear deformity can be detected at the earliest stage and missed diagnosis can be avoided by simultaneous hearing screening and ear deformity screening. During the window period of 7-14 d, by monitoring the self-healing rate of the affected ear excessive medical correction can be avoided. By hearing screening system statistics, ear shape malformation is not directly related to hearing loss.


Assuntos
Pavilhão Auricular , Perda Auditiva , Pavilhão Auricular/anormalidades , Orelha Externa , Perda Auditiva/diagnóstico , Perda Auditiva/etiologia , Testes Auditivos , Humanos , Recém-Nascido , Triagem Neonatal
4.
Osteoarthritis Cartilage ; 27(5): 726-736, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30391538

RESUMO

OBJECTIVE: This study was design to examine the diagnostic performance of cartilage oligomeric matrix protein (COMP), C-terminal cross-linking telopeptide of type II collagen (CTX-II), and matrix metalloproteinase-3 (MMP-3) as biomarker for knee and hip OA. METHODS: Systematic search on multiple databases was completed in January 2018 using certain keywords. COMP, CTX-II, MMP-3 levels in knee and hip OA patients and healthy individuals were collected and calculated. Differences between subgroups were expressed as standardized mean differences (SMD). Subgroup analyses were performed to compare COMP, CTX-II, and MMP-3 performance between measuring sources, genders, large and small sample size and diagnostic criteria for OA patients. RESULTS: A moderate performance of COMP in distinguishing between knee (SMD: 0.68; 95% confidence intervals (CI): 0.43-0.93; P < 0.0001) or hip (SMD: 0.25; 95% CI, 0.10, 0.40; P = 0.0008) OA patients and controls were found. CTX-II showed a moderated standardised mean differences (SMD) of 0.48 (95% CI, 0.32, 0.64; P < 0.0001) in the detection of knee OA and a large SMD of 0.76 (95% CI, 0.09, 1.42; P = 0.03) in diagnosing hip OA. A small SMD of 0.32 (95% CI, -0.03, 0.67; P = 0.07) was found for MMP-3 performance and the results did not reach statistic significance. Progression study revealed potential effectiveness of serum COMP in predicting OA progression. Subgroup analysis showed that serum COMP and urinary CTX-II performed better in male than female. Study size and diagnostic criteria did not significantly influence the pooled SMD, but they might be the sources of heterogeneity among studies. CONCLUSION: The overall results indicates that serum COMP and urinary CTX-II can distinguish between knee or hip OA patients and control subjects. Serum COMP is effective in predicting OA progression.Further researches with rigorous study design and a larger sample size are required to validate our findings.

5.
Artigo em Chinês | MEDLINE | ID: mdl-31914302

RESUMO

Objective:To analyze the hearing assessment characteristics and follow-up of some deafness gene screening homozygous infants in Zhuhai. Method:The clinical data of 28 newborns with homozygous mutations transferred to Zhuhai Maternal and Child Health Hospital from Feb. 1, 2015 to Oct. 25, 2018 in hospitals of Zhuhai City were retrospectively analyzed. All the children were screened for hearing. The hearing characteristics and long-term follow-up results of homozygous mutations at different gene sites were analyzed by auditory diagnosis and behavioral follow-up from 1 to 3 years. Result:Fourteen cases of GJB2 c.109G>A with a homozygous mutation, 11 cases passed the hearing screening, the audiological diagnosis was normal, and the behavior test and follow-up were normal from 1 to 3 years. Hearing screening was not passed in 3 newborns, mild to moderate abnormalities of single or bilateral ears were diagnosed by audiology, 1 000 Hz without positive, and middle ear lesions were diagnosed. Eight cases of GJB2 c.235del C homozygous mutation were followed up by behavioral audiometry and follow-up from 1 to 3 years after cure. Among them, 5 cases were diagnosed as severe hearing impairment of bilateral ears and 3 cases as mild and moderate hearing impairment. One case of GJB3 547G>A homozygous mutation was followed up for 1-3 years, and all of them failed to pass the follow-up of behavioral audiometry and follow-up. Four cases of SLC26A4 IVS7-2A>G, 1 case of SLC26A4 1229C>T homozygous mutation, all of them failed to pass the neonatal hearing screening. All the patients were diagnosed as severe hearing impairment of binaural hearing, and the follow-up of 1-3 years' follow-up did not pass the follow-up tests. Conclusion:GJB2 C.235del C, SLC26A4 IVS7-2A>G locus homozygous mutation infant hearing impairment was mainly severe hearing impairment in bilateral ears, and there was no change in 1-3 years follow-up. GJB2 C.109G A homozygous mutant infants had normal hearing, and it was suggested that they should be followed up closely. It is very important to give correct and reasonable genetic counseling to parents with GJB2 C.109G A homozygous mutation without unnecessary panic.


Assuntos
Conexinas , Surdez , Criança , Seguimentos , Humanos , Lactente , Recém-Nascido , Mutação , Estudos Retrospectivos
6.
Artigo em Chinês | MEDLINE | ID: mdl-30550147

RESUMO

Objective:To discuss the effect of obesity on the operation of thyroid gland surgery and surgical results.Method:The clinical data of 446 patients who underwent robot thyroid surgery through bilateral axillo-breast approach (BABA) from the General Hospital of Jinan Military region from February 2014 to November 2017 were analyzed retrospectively, and the patients were divided into BMI<25 kg/m²; group, BMI 25-30 kg/m²; group and BMI>30 kg/m²; group according to body mass index (BMI). The operative complications were compared between the three groups of benign and malignant patients, such as operation time, postoperative lead flow, postoperative hospitalization time, tumor size (malignant), lymph node metastasis (malignant), cosmetic satisfaction score, laryngeal nerve injury and parathyroid function decrease. Statistical methods using Variance analysis and χ² test to compare the differences between the two groups of indicators, difference is statistically significant (P<0.05).Result:The difference of operation time, postoperative average hospitalization time and postoperative drainage fluid volume in 3 groups was not statistically significant(P>0.05). ①Intraoperative and postoperative pathological results were benign: BMI<25 kg/m²; group 69 cases, BMI 25-30 kg/m²; group 48 cases, BMI>30 kg/m²; group 8 cases, temporary recurrent laryngeal nerve injury were 1 case, 0 case and 0 case respectively, temporary parathyroid function decrease 3 cases, 2 cases and 1 case. ②Intraoperative and postoperative pathological results were malignant:BMI<25 kg/m²; group 180 cases, BMI 25-30 kg/m²; group 119 cases,BMI>30 kg/m²; group 22 cases, temporary recurrent laryngeal nerve injury were 2 cases,1 case and 0 case respectively, temporary parathyroid dysfunction in 64 cases,29 cases and 5 cases respectively.③1 patient in BMI<25 kg/m²; group had lymphatic leakage after operation, 1 patient in BMI 25-30 kg/m²; group had subcutaneous tunnel hemorrhage, and 1 patient had lymphatic leakage during operation.Conclusion:For overweight or obese patients, the da Vinci robot thyroid operation is reliable, does not increase the risk of surgical complications, but also has a good cosmetic effect.

7.
Artigo em Chinês | MEDLINE | ID: mdl-30550149

RESUMO

Thyroid cancer is the most common malignant tumor in endocrine surgery. Surgery is the first choice for most patients with thyroid cancer. Da Vinci robot system as the auxiliary system is the most advanced endoscopic surgery, largely to fill the cavity mirror device cannot bend, complex operation and so on insufficiency, has now become an important way of surgical treatment of thyroid cancer, and its curative effect, high safety, but because of the economic cost is higher, is currently not widespread popularity.

8.
Artigo em Chinês | MEDLINE | ID: mdl-29902859

RESUMO

Stem cell is critical to regeneration of tissue or organ of human. How to promote repair or regeneration in the tissues/organ using its pluripotency is always an important issue. Lgr5-possitive cell is one type of the stem cell-like cells capable of pluripotent differentiation in various tissues/organs of both humans and mice. Current study showed that single or small amount Lgr5-possitive stem cells can grow and form a plurality of organs in 3D culture system, and some organs can present similar biological and physiological properties with the progenitor they were derived. These studies provided new insight into future orientation, for example, Lgr5-possitive inner ear cells were confirmed as inner ear pluripotent cells population, the experiences obtained from organoid studies of Lgr5-possitive cells have certainly showed potential in the future study of inner ear stem cells. This review will focus on the recent progress associated with Lgr 5-positive stem cells forming organoids in the 3D culture.


Assuntos
Diferenciação Celular , Organoides/crescimento & desenvolvimento , Células-Tronco Pluripotentes/fisiologia , Receptores Acoplados a Proteínas-G , Regeneração , Animais , Orelha Interna/citologia , Humanos , Camundongos , Células-Tronco Pluripotentes/química
9.
J Anim Physiol Anim Nutr (Berl) ; 102(5): 1210-1219, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-29806083

RESUMO

Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS-QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L-glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2-oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L-glutamine, L-isoleucine and L-leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.


Assuntos
Embrião de Galinha/metabolismo , Metabolômica , Proteômica , Tecido Adiposo , Animais , Galinhas , Cromatografia Líquida , Modelos Animais de Doenças , Humanos , Metabolômica/métodos , Obesidade , Proteômica/métodos
10.
Public Health ; 157: 121-126, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29524810

RESUMO

OBJECTIVES: Findings regarding the benefits of fruit and vegetables (FV) on weight control are inconsistent and little is known among Chinese populations. Therefore, we examined the relationship between change in FV consumption, weight, and change in body mass index (BMI) among Chinese adults, participants of the China Health and Nutrition Survey (CHNS). STUDY DESIGN: A prospective cohort study. METHODS: Two waves of CHNS conducted in 2006 and 2011 were used. Continuous FV consumption increase was considered as the exposure and changes in weight and BMI as outcomes. Change in FV consumption was categorized into quintiles. Analysis of covariance and multiple linear regression models, after controlling for potential confounders such as energy intake, physical activity, and smoking, were used to describe the relationship between change in FV consumption and change in weight and BMI. RESULTS: A total of 4357 participants aged 18-65 years were included in this study. The respective weight and BMI gains in male individuals were 1.81 kg and 0.73 kg/m2 in the fifth quintile of FV change relative to individuals in the first quintile (3.67 kg for weight gain and 1.48 kg/m2 for BMI gain). An increase in FV consumption by 100 g was associated with a 211 g weight loss (B = -2.11; 95% confidence interval [CI], -3.34, -0.89, P < 0.001) and a decrease in BMI by 0.94 kg/m2 (B = -0.94; 95% CI, -1.36, -0.46, P < 0.001) in men; and a 140 g weight loss (B = -0.14; 95% CI, -0.97, 0.69, P = 0.74) and a decrease in BMI by 0.29 kg/m2 BMI (B = -0.29; 95% CI, -0.63, 0.06, P = 0.11) in women. CONCLUSIONS: Increase in FV consumption was associated with statistically significant weight loss and decrease in BMI among Chinese men, and, although suggested, weight loss among women was not significant. Considering the protective effect of FV on human health, increasing FV consumption in the Chinese population is recommended.


Assuntos
Índice de Massa Corporal , Peso Corporal , Dieta/estatística & dados numéricos , Frutas , Verduras , Adolescente , Adulto , Idoso , China/epidemiologia , Dieta/efeitos adversos , Feminino , Inquéritos Epidemiológicos , Humanos , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , Obesidade/epidemiologia , Sobrepeso/epidemiologia , Estudos Prospectivos , Adulto Jovem
11.
Zhonghua Wai Ke Za Zhi ; 55(8): 570-573, 2017 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-28789504

RESUMO

Da Vinci surgical system is the most advanced minimally invasive surgical platform in the world, and this system has been widely used in cardiac surgery, urology surgery, gynecologic surgery and general surgery. Although the application of this system was relatively late in thyroid surgery, the number of thyroidectomy with Da Vinci surgical system is increasing quickly. Having reviewed recent studies and summarized clinical experience, compared with traditional open operation, the robotic thyroidectomy has the same surgical safety and effectiveness in selective patients with thyroid cancer. In this paper, several aspects on this novel operation were demonstrated, including surgical indications and contraindications, the approaches, surgical procedures and postoperative complications, in order to promote the rational application of Da Vinci surgical system in thyroidectomy.


Assuntos
Procedimentos Cirúrgicos Robóticos , Robótica , Neoplasias da Glândula Tireoide , Tireoidectomia , Humanos , Procedimentos Cirúrgicos Robóticos/métodos , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia/métodos
12.
Public Health ; 149: 65-70, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28554164

RESUMO

OBJECTIVES: To explore the association between obesity phenotype and the risk of hypertension among Chinese adults. STUDY DESIGN: A prospective cohort study. METHODS: Two waves of data were collected in 2009 and 2011 by the China Health Nutrition Survey. According to International Diabetes Federation and Chinese obesity criteria, participants were divided into four groups: metabolically healthy non-overweight/obesity (MHNO), metabolically healthy overweight/obesity (MHO), metabolically abnormal non-overweight/obesity (MANO), and metabolically abnormal overweight/obesity (MAO). Logistic regression model was performed to estimate the risk of hypertension with obesity phenotype. RESULTS: Among a total of 4604 adults aged 18-65 years at baseline, 467 developed hypertension during the 2-year follow-up period. After adjusting for several potential confounders, significantly increased risks for hypertension were found for participants in MHO (odd ratio [OR]: 1.78, 95% confidence interval [CI]: 1.39-2.27), MANO (OR: 1.71, 95% CI: 1.02-2.86), and MAO (OR: 3.35, 95% CI: 2.54-4.42) group compared with the MHNO group. CONCLUSION: Metabolically abnormal individuals, regardless of their body weight status, showed significantly higher risks for hypertension compared with healthy non-overweight/obese group. Furthermore, MHO individuals had significantly increased risk of incident hypertension.


Assuntos
Hipertensão/epidemiologia , Obesidade , Fenótipo , Adolescente , Adulto , Idoso , China/epidemiologia , Feminino , Inquéritos Epidemiológicos , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Medição de Risco , Adulto Jovem
13.
Nat Prod Res ; 30(5): 590-4, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-25835468

RESUMO

To investigate the bioactive constituents of the inflorescences of Scabiosa comosa and S. tschilliensis, which are used traditionally for liver diseases, we tested the antioxidant activity using 2,2'-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant potential (FRAP), and DPPH-ultra high performance liquid chromatography-mass spectrometer (UPLC-MS) assay. In addition, cell-based anti-HCV activity of the major compounds were evaluated. The plant extracts showed strong antioxidant activity. For the first time, 3,4-dicaffeoylquinic acid (DCQA), 3,5-DCQA and 4,5-DCQA were identified from genus Scabiosa. A UPLC-MS method in multiple reaction monitoring (MRM) mode was established to quantify 18 constituents in the inflorescences of Scabiosa. The 3,5-DCQA, chlorogenic acid and some glycosides of luteolin or apigenin were found to be the most abundant constituents. Chlorogenic acid and 3,5-DCQA showed excellent radical scavenging activity and demonstrated anti-HCV activity. These findings provided scientific evidences for the clinic use of this herbal medicine for liver diseases.


Assuntos
Antioxidantes/farmacologia , Antivirais/farmacologia , Dipsacaceae/química , Hepacivirus/efeitos dos fármacos , Antioxidantes/isolamento & purificação , Antivirais/isolamento & purificação , Compostos de Bifenilo/química , Ácido Clorogênico/análogos & derivados , Depuradores de Radicais Livres/farmacologia , Humanos , Luteolina , Picratos/química , Extratos Vegetais/química , Ácido Quínico/análogos & derivados , Espectrometria de Fluorescência
14.
Org Biomol Chem ; 13(13): 4070-9, 2015 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-25736157

RESUMO

This study investigated the unexpected decomposition and associated intermediates of compound 1, a specific member of a drug discovery library based on a monosaccharide scaffold. LC/MS and NMR spectroscopic analyses indicated that, under acidic conditions, 1 can be converted into the 4-aminogalactoside 2, due to cleavage of the 4-aminobutanoyl side chain. The reaction occurs most likely through an initial intramolecular amino­amide interaction, followed by an N- to O-acyl transfer of the side chain from C-4 to the C-6 position to form an ester intermediate (5), detectable by NMR, and subsequent hydrolysis. Similar decomposition reactions could be induced in selected compounds with similar structures, containing a free hydroxyl group at C-6 and a 4-aminobutanoyl side chain at C-4 of an aminogalactoside. Furthermore, three model compounds were synthesized without a C-6 hydroxyl group and with different length aminoalkanoyl side chains at the C-4 position. The model compounds all decomposed under acidic conditions, but at different rates and much slower when compared with compound 1, suggesting that both the C-6 hydroxyl group and the length of the side chain have an influence on stability.


Assuntos
Descoberta de Drogas , Monossacarídeos/química , Bibliotecas de Moléculas Pequenas/química , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio
15.
J Eur Acad Dermatol Venereol ; 29(1): 115-9, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24665959

RESUMO

BACKGROUND: China has a great variety of geographical and climatic conditions, and several cultural differences exist within the country; thus, understanding the regional and seasonal differences that cause skin sensitivities in this country is important. OBJECTIVE: The aim of this study was to assess skin sensitivity of women from six cities in China and from South Korea during the winter and summer seasons to aid the development of suitable and effective dermatological products. METHODS: This multicentre study included 754 healthy female volunteers, and was conducted in the winter (between January and March) and summer (between June and July) of 2011. Patch tests were performed using 0.5% sodium lauryl sulphate (SLS) aqueous solution and 0.15% retinol in 1,3-butylene glycol on the back of the volunteers. Simultaneously, stinging tests were performed on their cheeks by using 5% lactic acid solution and 0.001% capsaicin solution, each in a negative control vehicle (distilled water and 10% ethanol solution, respectively). RESULTS: The patch test results showed that the subjects in Beijing and Shenyang were more sensitive to SLS, retinol and lactic acid in the winter than were those in Guangzhou, Shanghai, Wuhan, Chengdu and South Korea. The stinging test results revealed that the subjects in Beijing were more neurosensitive to lactic acid in the winter; however, during the same season, the subjects from Shanghai and Guangzhou were significantly more neurosensitive to capsaicin. CONCLUSION: Our observations indicate that skin sensitivity differs considerably between women from different parts of China and South Korea. We recommend that these differences be considered during the development of cosmetic products in these countries.


Assuntos
Dermatite de Contato/epidemiologia , Estações do Ano , Adulto , Capsaicina/efeitos adversos , China/epidemiologia , Dermatite de Contato/etnologia , Dermatite de Contato/etiologia , Feminino , Voluntários Saudáveis/estatística & dados numéricos , Humanos , Ácido Láctico/efeitos adversos , Dor/induzido quimicamente , Testes do Emplastro , República da Coreia/epidemiologia , Fármacos do Sistema Sensorial/efeitos adversos , Testes de Irritação da Pele , Dodecilsulfato de Sódio/efeitos adversos , Tensoativos/efeitos adversos , Vitamina A/efeitos adversos , Vitaminas/efeitos adversos , Adulto Jovem
16.
Skin Res Technol ; 20(4): 498-502, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24665994

RESUMO

BACKGROUND: There are ethnic differences in the skin characteristics, also the skin is susceptible to be influenced by the external environment such as UV radiation and the climates. It can be shown that the skin in same race or twins varies by the environment. OBJECTIVES: This study was designed to investigate the skin characteristics and the early wrinkles of young Chinese women from four different regions, and to identify the correlation among the wrinkles, the other skin characteristics, and environmental conditions. METHODS: A total of 441 healthy Chinese women aged between 20 and 35 years participated in the study: 110 from Beijing, 110 from Shanghai, 111 from Wuhan, and 110 from Guangzhou. The skin hydration, sebum contents, TEWL, pH, elasticity, and wrinkles were measured on the crow's feet area. RESULTS: There were regional differences in the skin characteristics and the wrinkles. Beijing women had dry skin and more wrinkles, but Guangzhou women had high sebum contents, low pH, and less wrinkles (P < 0.01). Shanghai women's TEWL and Wuhan's women's skin elasticity were higher compared with that of women from other regions. The wrinkles' form (area, depth, and length) was different from region to region. Beijing women's wrinkles were deep and large, but Guangzhou women's wrinkles were shallow and small. The skin physical parameters that influenced the wrinkles were low sebum content and hydration, high TEWL, and pH (P < 0.05). CONCLUSION: In the Chinese women aged 20-35 years, the skin was influenced by the climates, so they had regionally a different skin. The skin hydration, sebum contents, TEWL, and pH can affect the early wrinkle formation than skin elasticity.


Assuntos
Água Corporal/metabolismo , Clima , Meio Ambiente , Envelhecimento da Pele/etnologia , Envelhecimento da Pele/fisiologia , Pele/química , Perda Insensível de Água/fisiologia , Adulto , China , Módulo de Elasticidade/fisiologia , Feminino , Humanos , Concentração de Íons de Hidrogênio , População Urbana/classificação , População Urbana/estatística & dados numéricos
17.
Genet Mol Res ; 11(1): 693-706, 2012 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-22535405

RESUMO

Two genetic linkage maps of cultivated maize inbred lines and teosinte species were constructed. One population comprised 81 F(2) individuals derived from a cross between maize inbred line B73 and Zea mays ssp parviglumis, while the second consisted of 63 backcross individuals from a cross of maize inbred line B73 with Z. mays ssp diploperennis. In the B73 x Z. mays ssp parviglumis F(2) population, 172 simple sequence repeat (SSR) markers were mapped to 10 chromosomes, which covered 2210.8 cM. In the B73 x Z. mays ssp diploperennis backcross population, 258 SSR markers were mapped to 10 chromosomes, covering 1357.7 cM. Comparison of the two maps revealed that the total map length of Z. mays ssp diploperennis covers 1357.7 cM, which is about 61.4% of that of Z. mays ssp parviglumis (2210.8 cM). Extensive segregation distortion regions were found on chromosomes 1, 2, 3, 5, 6, 7, and 10 in the B73 x Z. mays ssp parviglumis F(2) population and on chromosomes 1-5 and 8-10 in the B73 x Z. mays ssp parviglumis backcross population. Segregation distortion analysis confirmed that the segregation distortion ratio in the interspecific population B73 x Z. mays ssp diploperennis was higher than in B73 x Z. mays ssp parviglumis. We found that the recombination distances are highly variable in these genetic crosses between cultivated and wild species of maize.


Assuntos
Segregação de Cromossomos , Cruzamentos Genéticos , Zea mays/genética , Mapeamento Cromossômico , Cromossomos de Plantas , Ligação Genética , Genótipo , Repetições de Microssatélites , Polimorfismo Genético , Recombinação Genética
18.
Public Health ; 122(12): 1425-32, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18752814

RESUMO

OBJECTIVE: To investigate the role of nutrition in children's respiratory health in subjects participating in a study on air pollution and respiratory health. STUDY DESIGN: Cross-sectional survey. METHODS: The relationships between consumption of fresh fruit, vegetables and milk and pulmonary function (forced expiratory volume in 1 s, FEV(1)) and respiratory symptoms (phlegm with cold, phlegm without cold, cough with cold, and cough without cold) were examined in 2228 children (age 10.07+/-0.86 years) in Guangzhou, China. RESULTS: A positive association was found between consumption of leafy vegetables and lung function in children. Subjects with the highest consumption of leafy vegetables had significant higher FEV(1) values than subjects who ate leafy vegetables less than once per week (1.68 l vs 1.56 l; P=0.047). Children with high consumption of fresh fruit and milk had higher lung function values than children with low consumption, although the differences were not statistically significant. Milk consumption was a protective factor for phlegm with cold, cough with cold, and cough without cold [odds ratio (OR) 0.62, 95% confidence interval (CI) 0.39-0.97; OR 0.53, 95% CI 0.36-0.79; OR 0.43, 95% CI 0.21-0.92, respectively, in the highest intake group]. Consumption of vegetables was a protective factor for cough with cold (P for trend=0.003) and cough without cold (P for trend=0.028). Consumption of fresh fruit was marginally associated with reduced prevalence of respiratory symptoms. CONCLUSIONS: This study suggests that dietary nutrients are important protective factors for children's respiratory health. Following-up these children may provide more evidence to confirm these associations.


Assuntos
Estado Nutricional , Sistema Respiratório , Doenças Respiratórias/epidemiologia , Adolescente , Animais , Criança , Bem-Estar da Criança , Pré-Escolar , China/epidemiologia , Intervalos de Confiança , Estudos Transversais , Dieta , Comportamento Alimentar , Feminino , Frutas , Nível de Saúde , Inquéritos Epidemiológicos , Humanos , Masculino , Leite , Razão de Chances , Testes de Função Respiratória , Doenças Respiratórias/fisiopatologia , Inquéritos e Questionários , Verduras
19.
J Asian Nat Prod Res ; 8(4): 373-7, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16864450

RESUMO

A new phenolic glycoside, 6'-[(E)-2''-hydroxymethyl, 2''-butenoyl] arbutin (1), and two known phenolic glycosides, 6'-[(E)-4''-hydroxycinnamoyl] arbutin (2) and 6'-[(E)-3'',4''-dihydroxycinnamoyl] arbutin (3), were isolated from the leaves of Heliciopsis lobata (Merr.) Sleum. Their structures were elucidated by various spectroscopic methods including 2D NMR spectroscopy.


Assuntos
Arbutina/análogos & derivados , Glicosídeos/química , Fenóis/química , Folhas de Planta/química , Proteaceae/química , Arbutina/química , Arbutina/isolamento & purificação , Linhagem Celular Tumoral , Glicosídeos/isolamento & purificação , Humanos , Estrutura Molecular , Fenóis/isolamento & purificação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA