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1.
Genes (Basel) ; 11(1)2020 01 10.
Artigo em Inglês | MEDLINE | ID: mdl-31936856

RESUMO

To elucidate the effect of low temperature on anthocyanin biosynthesis in purple head Chinese cabbage, we analyzed anthocyanin accumulation and related gene expression in the seedlings of purple head Chinese cabbage, white head parent Chinese cabbage, and its purple male parent under a normal 25 °C temperature and a low 12 °C temperature. Anthocyanin accumulation in purple lines was strongly induced by low temperature, and the total anthocyanin content of seedlings was significantly enhanced. In addition, nearly all phenylpropanoid metabolic pathway genes (PMPGs) were down-regulated, some early biosynthesis genes (EBGs) were up-regulated, and nearly all late biosynthesis genes (LBGs) directly involved in anthocyanin biosynthesis showed higher expression levels in purple lines after low-temperature induction. Interestingly, a R2R3-MYB transcription factor (TF) gene 'BrMYB2' and a basic-helix-loop-helix (bHLH) regulatory gene 'BrTT8' were highly up-regulated in purple lines after low temperature induction, and two negative regulatory genes 'BrMYBL2.1' and 'BrLBD38.2' were up-regulated in the white line. BrMYB2 and BrTT8 may play important roles in co-activating the anthocyanin structural genes in purple head Chinese cabbage after low-temperature induction, whereas down-regulation of BrMYB2 and up-regulation of some negative regulators might be responsible for white head phenotype formation. Data presented here provide new understanding into the anthocyanin biosynthesis mechanism during low temperature exposure in Brassica crops.

2.
Nanoscale ; 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31994580

RESUMO

Metamaterial-based electromagnetic absorbers have attracted much attention recently, but most previous realizations suffer from issues of narrow bandwidth, time-consuming and high-cost fabrication methods, and/or fixed functionalities, and so are unfavorable for practical applications. Here, we demonstrate experimentally a large-scale, broadband, polarization-independent, and tunable metamaterial absorber, which works for both visible and near-infrared light. A lithography-free and low-cost method was utilized to fabricate a centimeter-sized metamaterial sample in a metal-insulator-metal (MIM) configuration with nano-scale precision, in which a phase-change material, Ge2Sb2Te5 (GST), was adopted as the insulating spacer of the MIM structure. With two different resonance mechanisms working together, the proposed device was shown to exhibit high absorptivity (>80%) within a broad wavelength band (480-1020 nm). By thermally tuning the phase state of the GST layer, we can dramatically enlarge the working bandwidth of the metamaterial absorber by shifting one absorption peak by about 470 nm. These findings may stimulate many potential applications in, for example, solar cells, energy harvesting, smart sensing/imaging, and color printing.

3.
Oncogene ; 2019 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-31831835

RESUMO

The original version of this Article omitted the following from the Acknowledgements: Professor Stebbing sits on SABs for Celltrion, Singapore Biotech, Vor Biopharma, TLC Biopharmaceuticals and Benevolent AI, has consulted with Lansdowne partners, Vitruvian and Social Impact Capital and Chairs the Board of Directors for BB Biotech Healthcare Trust and Xerion Healthcare. This has now been corrected in both the PDF and HTML versions of the Article.

4.
PLoS One ; 14(11): e0224968, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31743354

RESUMO

OBJECTIVE: This study was aimed to examine the effect of feeding patterns on growth and nutritional status of children aged 0~24 months. METHODS: We conducted a cohort study with an initial sample of 927 children. Considering the follow-up losses, 903, 897, 895, 897, 883, 827 and 750 children were followed up at 1, 3, 6, 8, 12, 18 and 24 months, respectively. Children were grouped according to exclusive breastfeeding (EBF) duration in the first 6 months: (1) never EBF; (2) EBF ≤ 3 months: EBF ≤ 3 months and stopped BF after 3 months or EBF ≤ 3 months and BF = 6 months or EBF ≤ 3 months and BF after 3 months, had formula and/or solids; (3) EBF for 3 ~ 6 months: BF < 3 months and EBF for 3 ~ 6 months or EBF for 3 ~ 6 months and BF < 3 months, had formula and/or solids; (4) EBF = 6 months. We used Z-scores to evaluate the growth and nutritional status of children, used the generalized estimation equation to compare the difference between feeding patterns. RESULTS: The generalized estimation equation results showed that Weight-for-age Z-score (WAZ), Length-for-age Z-score (LAZ), and Weight-for-length Z-score (WLZ) in different feeding patterns had statistical significance. The WAZ in EBF for 6 months group was higher in the first 8 months, in never EBF group was higher after 12 months old; the LAZ in EBF for 6 month group was lower than other groups; the WLZ in EBF for 6 months group was higher than EBF for 3 ~ 6 months group. The EBF ≤ 3 months group had higher underweight, stunting, and wasting rates. The EBF for 6 months had a higher stunting rate; the never EBF and EBF for 6 months groups had higher overweight and obesity rates. CONCLUSIONS: In conclusion, different feeding patterns affect growth and nutritional status in children, so proper guidelines should be implemented to improve nutritional status and promote the growth of children.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31731641

RESUMO

This study aimed to examine the associations between the duration of folic acid (FA) supplementation, gestational diabetes mellitus (GDM), and adverse birth outcomes. A total of 950 mother-offspring pairs participated in the cohort study during 2015 in Changsha, China. The data were collected through home visits and perfected by maternal and child healthcare handbooks. Generalized linear models and stratified analyses were used for statistical analyses. The incidence of GDM in our cohort was 10.2%. FA supplementation for ≥3 months before pregnancy was associated with an increased risk of GDM (adjusted relative risk (aRR): 1.72; 95% CI: 1.17-2.53) and decreased risk of small-for-gestational-age (SGA) birth (aRR: 0.40; 95% CI: 0.18-0.88). In the group of FA supplementation for ≥3 months during pregnancy, GDM was associated with an increased risk of cesarean delivery (aRR: 1.36; 95% CI: 1.06-1.75) and macrosomia (aRR: 2.11; 95% CI: 1.06, 4.20), but the aRRs were lower than the RRMH 1.53 (95% CI: 1.01-2.34) and 2.43 (95% CI: 1.27-4.66). Our study suggested that the longer duration of FA supplementation before pregnancy might increase the risk of GDM, but decrease the risk of SGA birth. Longer duration of FA supplementation during pregnancy had beneficial effects on birth outcomes in women with GDM. Further studies should consider a larger sample size to confirm these findings.

6.
Oncogene ; 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754213

RESUMO

EGFR-mutant non-small-cell lung cancer (NSCLC) patients inevitably develop drug resistance when treated with EGFR tyrosine kinase inhibitors (TKIs). Systematic genetic analysis is important to understand drug-resistant mechanisms; however, the clinical significance of co-occurring genetic alterations at baseline, co-acquired mutations at progressive disease (PD), and the clonal evolution remain underinvestigated. We performed targeted sequencing of pre-treatment and PD tumor samples from 54 EGFR-mutant NSCLC patients. Ten additional patients were sequenced using whole-exome sequencing to infer the clonal evolution patterns. We observed a domain-dependent effect of PIK3CA mutation at baseline on patient progression-free survival (PFS). In addition, at baseline, 9q34.3/19p13.3 (NOTCH1/STK11/GNA11) showed a co-deletion pattern, which was associated with a significantly worse PFS (p = 0.00079). T790M-postive patients with other concurrent acquired oncogenic mutations had a significantly shorter PFS (p = 0.005). Besides acquired T790M mutation, chromosomal instability (CIN) related genes, including AURKA and TP53 alterations, were the most frequently acquired events. CIN significantly increased during TKI treatment in T790M-negative patients and is a candidate resistance mechanism to the first-generation TKIs. Clonal evolution analyses suggest that the composition and relationship among resistant subclones, particularly relationship with T790M subclone, affect patients' outcomes. Overall, our findings of novel co-occurring alterations and clonal evolution patterns can be served as predictive biomarkers to stratify patients and help to better understand the drug-resistant mechanism to TKIs.

7.
BMC Pregnancy Childbirth ; 19(1): 388, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31660900

RESUMO

BACKGROUND: The first few weeks after childbirth are critical, as women may encounter lactation problems and postpartum depression during this period. However, it is still unclear whether early breastfeeding behaviours are related to the symptoms of postnatal depression (PND) in Chinese populations. Therefore, the current study aimed to investigate the association between symptoms of PND and infant feeding practices based on a large-scale Chinese cohort. METHODS: A prospective study of the community-based cohort was conducted from January 2015 to December 2016. Infant feeding outcomes, including exclusive/partial breastfeeding and formula feeding, were assessed according to the WHO guidelines. Symptoms of PND were assessed by the Edinburgh Postnatal Depression Scale at 4 weeks postpartum. Multivariate generalized estimating equation models were applied to investigate the associations between depressive symptoms and infant feeding behaviours. RESULTS: A total of 956 mother-infant pairs were included. Fifty-six mothers presented screen-positive symptoms of PND with a cut-off ≥10. The percentage of early breastfeeding initiation was 75.8%, while the average duration of exclusive breastfeeding was 3.90 ± 2.33 months. Postnatal depressive symptoms were associated with a shorter breastfeeding duration (8.02 vs. 6.32 months, P < 0.05) and earlier formula introduction (4.98 vs. 3.60 months, P < 0.05). After adjustments were made for covariates, postnatal depressive symptoms were associated with an increased risk of the discontinuation of exclusive and partial breastfeeding (ß = - 0.049, P = 0.047 and ß = - 0.082, P = 0.006, respectively). Compared to mothers without symptoms of PND, mothers with depressive symptoms were more likely to supplement formula for their infants in the first year of life (ß =0.074, P = 0.016). These associations were still significant in the sensitivity analyses, using an EPDS cut-off of ≥13. CONCLUSIONS: Our findings indicate that depressive symptoms at 4 weeks postpartum are associated with the cessation of exclusive and partial breastfeeding duration and the introduction of formula in the 12 months of delivery. Early psychosocial assessment and social support should be offered to mothers in the early postpartum period to indirectly prevent adverse breastfeeding outcomes.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31535987

RESUMO

Contrast enhanced ultrasound is a radiation-free imaging modality which uses encapsulated gas microbubbles for improved visualization of the vascular bed deep within the tissue. It has recently been used to enable imaging with unprecedented subwavelength spatial resolution by relying on super-resolution techniques. A typical preprocessing step in super-resolution ultrasound is to separate the microbubble signal from the cluttering tissue signal. This step has a crucial impact on the final image quality. Here, we propose a new approach to clutter removal based on robust principle component analysis (PCA) and deep learning. We begin by modeling the acquired contrast enhanced ultrasound signal as a combination of low rank and sparse components. This model is used in robust PCA and was previously suggested in the context of ultrasound Doppler processing and dynamic magnetic resonance imaging. We then illustrate that an iterative algorithm based on this model exhibits improved separation of microbubble signal from the tissue signal over commonly practiced methods. Next, we apply the concept of deep unfolding to suggest a deep network architecture tailored to our clutter filtering problem which exhibits improved convergence speed and accuracy with respect to its iterative counterpart. We compare the performance of the suggested deep network on both simulations and in-vivo rat brain scans, with a commonly practiced deep-network architecture and with the fast iterative shrinkage algorithm. We show that our architecture exhibits better image quality and contrast.

9.
Ann Vasc Surg ; 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31536795

RESUMO

BACKGROUND: This study aims to present the performance data on stent-graft and multilayer bare stents (MBS) joint technique in the treatment of high-risk thoracoabdominal aortic aneurysm (TAAA). METHODS: From May 2012 to December 2015, 8 selective TAAA cases (ages 46-75 years) ineligible for surgical repair underwent the stent-graft and MBS joint procedure, and were closely followed up for a median of 32 months (range 14-58). Using computed tomography images, the aneurysm size, luminal blood flow diameter, and the covered visceral branches were analyzed. RESULTS: Technical success was achieved in all patients (100%, 8/8). Twenty-four visceral branches were covered by MBS in total. There was no complication or death during hospital stay. During follow-up period, no death or complication occurred. Aneurysm shrinkage (maximum diameter decrease ≥5 mm) was observed in 7 patients. No aneurysm expansion was observed. Total aneurysm sac thrombosis was observed in all patients. The majority of covered side branches (23/24) were successfully preserved. No visceral ischemia or bleeding complications was observed during follow-up. CONCLUSIONS: Total endovascular repair of TAAA using stent-graft and MBS joint technique may be a safe and effective alternative in high surgical risk patients. More approving clinical evidences about the safety and efficacy of this procedure are anticipated.

10.
Research (Wash D C) ; 2019: 1849272, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31549047

RESUMO

Metasurfaces, ultrathin metamaterials constructed by planar meta-atoms with tailored electromagnetic (EM) responses, have attracted tremendous attention due to their exotic abilities to freely control EM waves. With active elements incorporated into metasurface designs, one can realize tunable and/or reconfigurable metadevices with functionalities controlled by external stimuli, opening a new platform to dynamically manipulate EM waves. In this article, we briefly review recent progress on tunable/reconfigurable metasurfaces, focusing on their working mechanisms and practical applications. We first describe available approaches, categorized into different classes based on external stimuli applied, to realize homogeneous tunable/reconfigurable metasurfaces, which can offer uniform manipulations on EM waves. We next summarize recent achievements on inhomogeneous tunable/reconfigurable metasurfaces with constitutional meta-atoms locally tuned by external knobs, which can dynamically control the wave-fronts of EM waves. We conclude this review by presenting our own perspectives on possible future directions and existing challenges in this fast developing field.

11.
J Food Biochem ; 43(11): e12998, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31373040

RESUMO

Jujube honey, a very popular honey in China, has been shown to own numerous biological properties. However, the anticancer effect and the underlying mechanisms of jujube honey in HepG2 cells have not been investigated to date. This study was designed to evaluate jujube honey-induced apoptosis and its molecular mechanism in HepG2 and the following results were obtained. Firstly, treatment with jujube honey blocked cell cycle progression at the G0/G1 phase, reduced the mitochondrial membrane potential (Δψm), induced DNA damage, and upregulates p53 expression. Secondly, pro-apoptotic proteins Bax and Bad were upregulated and antiapoptotic proteins Bcl-2 and Bcl-xL were downregulated. Lastly, caspase activation and apoptotic death occurred in HepG2 cells. In conclusion, this study showed that jujube honey induces HepG2 cell apoptosis via DNA damage, p53 expression, and caspase activation. PRACTICAL APPLICATIONS: We demonstrate the molecular mechanism by which jujube honey triggers apoptosis in HepG2 cells. This new insight provided useful information on the use of jujube honey as a potential therapeutic and preventive agent.

12.
Breastfeed Med ; 14(9): 654-661, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31368783

RESUMO

Background: With births generated with assisted reproductive techniques (ARTs) increasingly, the effect of ARTs on infant feeding behaviors is an essential topic to explore. However, limited literature focused on this topic. The objective of this study is to examine the effect of ARTs on infant feeding behaviors. Materials and Methods: Participants, including 41 mothers who conceived with ARTs and 935 conceived spontaneously, were drawn from a prospective birth cohort between January 2015 and December 2015. The participants were followed up at 1, 3, 6, 8, and 12 months postpartum at their residences through a face-to-face interview. Feeding behaviors, including breastfeeding initiation, partial/exclusive breastfeeding duration and formula introduction were assessed using World Health Organization-recommended definitions. Cox and logistic regression models were applied to examine the association of assisted conception with feeding behaviors. Results: Breastfeeding initiation rates were comparable between women who conceived with and without ARTs (70.7% versus 76.0%). Breastfeeding duration and formula introduction were significantly shorter and earlier in assisted conceptions in univariate analyses at 6 months postpartum [crude hazard ratios (HRs), 95% confidence intervals (CIs) 2.02 (1.03-3.84), and 1.63 (1.09-2.44)]. However, after controlling for covariates, assisted conception was only associated with shorter breastfeeding duration at 6 months postpartum [adjusted HR 1.99, 95% CI 1.05-3.80], no significant differences were found in infant feeding outcomes at 12 months postpartum among women with ART conception versus general conception. Mothers who conceived through ARTs with cesarean deliveries were at the highest risks of shorter breastfeeding duration and earlier formula introduction than mothers who conceived spontaneously with vaginal deliveries. Conclusions: Mothers who conceive through ARTs are associated with shorter breastfeeding durations for the first 6 months, compare to mothers who conceive spontaneously. Furthermore, cesarean delivery has a joint effect with the treatment of ARTs on promoting the development of poor feeding behaviors.

13.
Med Phys ; 46(9): 3864-3876, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31314917

RESUMO

PURPOSE: High-intensity focused ultrasound (HIFU)-mediated drug release becomes a promising therapeutic technique for treatment of cancer, which has merits of deep penetration, noninvasive approach and nonionizing radiation. However, conventional thermocouple-based approach for treatment monitoring would encounter big challenges such as the viscous heating artifact and difficulty in monitoring in the deep region. In this study, we develop an effective method based on thermal strain imaging (TSI) for the evaluation of HIFU-mediated drug release. METHODS: Both phantom experiments and preliminary animal experiments were performed to investigate the feasibility of the proposed approach. Doxorubicin (DOX)-loaded cerasomes (HIFU and temperature-sensitive cerasomes, HTSCs) were prepared. In the phantom experiments, the HTSC solution is contained inside a cylindrical chamber within a tissue-mimicking phantom. In the animal experiments, the HTSCs are intravenously injected into tumor-bearing mice. An HIFU transducer is used to trigger DOX release from the HTSCs within the phantom or mice, and TSI is performed during HIFU heating. In the phantom experiments, the accuracy of temperature estimation using TSI is validated by measuring with a thermocouple. In animal experiments, the spatial consistency between the distribution of DOX released within the tumor and the location of the heating region estimated by TSI is validated using a spectrofluorophotometer. RESULTS: In the phantom experiments, the HTSCs show a burst release of DOX when the temperature of the HTSC solution estimated by TSI reaches about 42°C, which is in agreement with the condition for drug release from the HTSCs. The temperature estimation using TSI has high accuracy with error below 2.5%. In animal experiments, fluorescence imaging of the tumor validates that the heating region of HIFU could be localized by the low-strain region of TSI. CONCLUSION: The present framework demonstrates a reliable and effective solution to the evaluation of HIFU-mediated local drug delivery.


Assuntos
Liberação Controlada de Fármacos , Imagem Molecular/instrumentação , Temperatura Ambiente , Ondas Ultrassônicas , Adenocarcinoma/diagnóstico por imagem , Adenocarcinoma/tratamento farmacológico , Animais , Linhagem Celular Tumoral , Estudos de Viabilidade , Neoplasias Mamárias Experimentais/diagnóstico por imagem , Neoplasias Mamárias Experimentais/tratamento farmacológico , Camundongos , Imagens de Fantasmas , Transdutores
14.
BMC Pediatr ; 19(1): 239, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31315604

RESUMO

BACKGROUND: Few prospective birth cohort studies are available on the effects of prenatal and early-life exposures on food allergy and eczema among Chinese children. The aim of this study was to evaluate the influence of prenatal and early-life exposures on food allergy and eczema during the first year of life in a prospective birth cohort study. METHODS: This study was based on a prospective, observational birth cohort of 976 mother-child pairs in three Streets in Changsha, China from January to December 2015. Data on prenatal, early-life exposures and allergic outcomes were obtained from questionnaires collected at birth, and 1, 3, 6, 8, and 12 months of age. Multivariate logistic regression models were performed to estimate the effects of prenatal and early-life exposures on food allergy and eczema. RESULTS: Common risk factors for food allergy and eczema in infancy were parental history of allergy, while moderate eggs consumption (3-4 times/week) during pregnancy was protective for both of them compared with low consumption (≤ 2 times/week). Factors only associated with food allergy were maternal aquatic products consumption during pregnancy, number of older siblings and age of solid food introduction, whereas factors only associated with eczema were maternal milk or milk products consumption during pregnancy, maternal antibiotic exposure during pregnancy, season of birth and antibiotic exposure through medication during the first year of life. CONCLUSION: Our study suggests that factors associated with food allergy and eczema are multifaceted, which involving hereditary, environmental and nutritional exposures. Furthermore, differential factors influence the development of food allergy and eczema in infants.

15.
FASEB J ; 33(9): 10165-10176, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31207192

RESUMO

Gastrointestinal toxicity limits the clinical application of abdominal and pelvic radiotherapy and currently has no effective treatment. Intestinal leucine-rich-repeat-containing GPCR 5 (Lgr5)-positive stem cell depletion and loss of proliferative ability due to radiation may be the primary factors causing intestinal injury following radiation. Here, we report the critical role of ß-arrestin1 (ßarr1) in radiation-induced intestinal injury. Intestinal ßarr1 was highly expressed in radiation enteritis and in a radiation model. ßarr1 knockout (KO) or knockdown mice exhibited increased proliferation in intestinal Lgr5+ stem cell, crypt reproduction, and survival following radiation. Unexpectedly, the beneficial effects of ßarr1 deficiency on intestinal stem cells in response to radiation were compromised when the endoplasmic reticulum stress-related protein kinase RNA-like ER kinase (PERK)/eukaryotic initiation factor-2α (eIF2α) pathway was inhibited, and this result was further supported in vitro. Furthermore, we found that ßarr1 knockdown with small interfering RNA significantly enhanced intestinal Lgr5+ stem cell proliferation after radiation via directly targeting PERK. ßarr1 offers a promising target for mitigating radiation-induced intestinal injury.-Liu, Z., Jiang, J., He, Q., Liu, Z., Yang, Z., Xu, J., Huang, Z., Wu, B. ß-Arrestin1-mediated decrease in endoplasmic reticulum stress impairs intestinal stem cell proliferation following radiation.

16.
Molecules ; 24(9)2019 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-31086027

RESUMO

Naodesheng (NDS) is a widely used traditional Chinese medicine (TCM) prescription for the treatment of ischemic stroke. A combination of 10 components is derived from NDS. They are: Notoginsenoside R1, ginsenoside Rg1, ginsenoside b1, ginsenoside Rd, hydroxysafflor yellow A, senkyunolide I, puerarin, daidzein, vitexin, and ferulic acid. This study aimed to investigate the protective effect of the ten-component combination derived from NDS (TCNDS) on ischemic stroke rats with a middle cerebral artery occlusion (MCAO) model by integrating an NMR-based metabonomics approach with biochemical assessment. Our results showed that TCNDS could improve neurobehavioral function, decrease the cerebral infarct area, and ameliorate pathological features in MCAO model rats. In addition, TCNDS was found to decrease plasma lactate dehydrogenase (LDH) and malondialdehyde (MDA) production and increase plasma superoxide dismutase (SOD) production. Furthermore, 1H-NMR metabonomic analysis indicated that TCNDS could regulate the disturbed metabolites in the plasma, urine, and brain tissue of MCAO rats, and the possible mechanisms were involved oxidative stress, energy metabolism, lipid metabolism, amino acid metabolism, and inflammation. Correlation analysis were then performed to further confirm the metabolites involved in oxidative stress. Correlation analysis showed that six plasma metabolites had high correlations with plasma LDH, MDA, and SOD. This study provides evidence that an NMR-based metabonomics approach integrated with biochemical assessment can help to better understand the underlying mechanisms as well as the holistic effect of multiple compounds from TCM.


Assuntos
Isquemia Encefálica/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Espectroscopia de Ressonância Magnética/métodos , Metabolômica/métodos , Acidente Vascular Cerebral/tratamento farmacológico , Animais , Apigenina/uso terapêutico , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Isquemia Encefálica/sangue , Isquemia Encefálica/urina , Ginsenosídeos/uso terapêutico , Infarto da Artéria Cerebral Média , Isoflavonas/uso terapêutico , L-Lactato Desidrogenase/sangue , Masculino , Malondialdeído/sangue , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Acidente Vascular Cerebral/sangue , Acidente Vascular Cerebral/urina , Superóxido Dismutase/sangue
17.
Lung Cancer ; 131: 62-68, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31027700

RESUMO

PURPOSE: Anaplastic lymphoma kinase (ALK) is now a validated kinase target in non-small cell lung cancer (NSCLC). We implemented three ALK laboratory methodologies: fluorescence in situ hybridization (FISH), immunohistochemistry (IHC) and next-generation sequencing (NGS) to detect EML4-ALK fusions and compared the predictive value for Crizotinib efficacy in ALK-positive patients. METHODS: 55 ALK positive patients confirmed by at least one method were enrolled in the present study, of whom 45 cases were assessed by FISH, IHC and NGS concurrently, and another 10 cases only received IHC and NGS assessment for ALK status. RESULTS: IHC presented the uppermost positive rate (94.5%), followed by NGS (92.7%) and FISH(82.4%), among which IHC and NGS had the highest concordance rate of 87.3%. No difference was detected in ORR, DCR and PFS of ALK positive cases defined in three groups. Notably, NGS positive patients were correlated with a higher DCR and longer PFS compared to NGS negative cases (P = 0.02 and P = 0.09), while FISH and IHC status were not distinguishing in predicting the outcome of Crizotinib. TP53 concurrent mutation might reduce responsiveness to Crizotinib and worsen prognosis in ALK-rearranged NSCLC. CONCLUSION: FISH present a certain false-negative rate although considered the gold standard. Ventana-D5F3 IHC is qualified as a screening tool, while NGS positive may predict clinical benefit of Crizotinib more accurately, allowing efficient test for specific variants and concurrent genomic alterations.

18.
Artigo em Inglês | MEDLINE | ID: mdl-30925697

RESUMO

BACKGROUND: The association of maternal parity, pre-pregnancy body mass index (BMI) and gestational weight gain (GWG) with childhood weight status has been well studied; however, little is known about these factors with respect to the rate of weight changes in early childhood. METHODS: This study was based on a prospective longitudinal study. The follow-up surveys were conducted at the ages of 1, 3, 6, 8, 12, and 18 months. Child weight was investigated twice at each wave. Data on maternal parity, pre-pregnancy weight and height were collected at baseline. The latent growth curve model was used to examine the effects of interested predictors on the trajectory of weight in early childhood. RESULTS: Finally, 893 eligible mother-child pairs were drawn from the cohort. In adjusted models, multiparas were associated with higher birth weight (ß = 0.103) and slower weight change rate of children (ß = -0.028). Pre-conception BMI (ß = 0.034) and GWG (ß = 0.014) played important roles in the initial status of child weight but did not have effects on the rate of weight changes of the child. CONCLUSIONS: Multiparous pregnancy is associated with both higher mean birth weight and slower weight-growth velocity in early childhood, while pregravid maternal BMI and GWG are only related to the birth weight.


Assuntos
Peso ao Nascer/fisiologia , Índice de Massa Corporal , Desenvolvimento Infantil/fisiologia , Ganho de Peso na Gestação/fisiologia , Paridade/fisiologia , Adulto , Estudos de Coortes , Família , Feminino , Humanos , Lactente , Estudos Longitudinais , Masculino , Gravidez , Estudos Prospectivos , Ganho de Peso
19.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(1): 59-66, 2019 Jan 28.
Artigo em Chinês | MEDLINE | ID: mdl-30837404

RESUMO

OBJECTIVE: To investigate the current status of the postpartum weight retention (PPWR) in Kaifu District of Changsha, and explore the influential factors with PPWR based on the generalized estimating equation model.
 Methods: A cluster sampling method was applied to select women who gave birth at the health service centers of 3 street communities in Kaifu District of Changsha during 2015. According to the inclusion and exclusion criteria, 783 cases were finally included in the study. Data on PPWR were collected prospectively by using the self-made questionnaire at 1, 3, 6 and 8 months after childbirth. Analysis were performed to investigate the associations between PPWR and its potential factors with generalized estimation equation model.
 Results: The mean PPWR of women was gradually decreased with the increase of postpartum time, and 34.6% of them have returned to the pre-pregnancy weight in the 8 months postpartum. Our findings revealed that gestation weight gain (GWG), pre-pregnancy body mass index, feeding patterns, and delivery mode were significantly associated with maternal PPWR (P<0.05). In contrast, maternal age, educational level, per capita income of family, parity and postpartum depression were not contributed to PPWR (P>0.05).
 Conclusion: GWG is one of the most important predictors for PPWR. The key to reducing PPWR is to control GWG systematically. Early targeted interventions and health education should be taken to prevent women from excessive PPWR at the first-year postpartum, in particular to the women who underwent cesarean deliveries and breastfed their infants. It is conducive to reduce the risks of overweight or obesity caused by PPWR.


Assuntos
Ganho de Peso na Gestação , Índice de Massa Corporal , Feminino , Humanos , Obesidade , Sobrepeso , Período Pós-Parto , Gravidez
20.
Artigo em Inglês | MEDLINE | ID: mdl-30703018

RESUMO

Tissue motion estimation is an essential step for ultrasound elastography. Our previous study has shown that the affine-model-based optical flow (OF) method outperforms the normalized cross-correlation-based block matching (BM) method in motion estimation. However, the quality of lateral estimation using OF is still low due to inherent limitation of ultrasound imaging. BM-based spatial angular compounding (SAC) has been developed to obtain better motion estimation. In this paper, OF-based SAC (OF-SAC) is proposed to further improve the performance of lateral (and axial) estimation, and it is compared with BM-based SAC (BM-SAC). Plane wave as well as focused wave is transmitted in both simulations and phantom experiments on a linear array. In order to compare the performance quantitatively, the root-mean-square error (RMSE) of axial/lateral displacement and strain, and signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) of axial/lateral strain are used as the evaluation criteria in the simulations. In the phantom experiments, the SNR and CNR are used to assess the quality of axial/lateral strain. The results show that for both OF and BM, SAC improves the performance of motion estimation, regardless of using plane or focused wave transmission. More importantly, OF-SAC is shown to outperform BM-SAC with lower RMSE, higher SNR, and higher CNR. In addition, preliminary in vivo experiments on the carotid artery of a healthy human subject also prove the superiority of OF-SAC. These results suggest that OF-SAC is preferred for both axial and lateral motion estimation to BM-SAC.

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