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1.
Vet Parasitol ; 279: 109059, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32112975

RESUMO

The entomopathogenic fungi Metarhizium anisopliae is highly pathogenic toward arthropods. Here, we evaluated the efficacy of a commercial formulation of M. anisopliae against P. ovis var. cuniculi in vivo and in vitro and explored the acaricidal mechanism of M. anisopliae by determining the antioxidant/detoxification-related enzymes activities including glutathione S-transferase (GST), superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in mites. The results showed that M. anisopliae had high acaricidal activity against P. ovis var. cuniculi in vitro, in a time- and dose-dependent manner, with 83.33 % mortality at day 9 and a median lethal time (LT50) of 6.10 days after applying 6.14 × 109 conidia/ml of M. anisopliae. In vivo experiments, M. anisopliae achieved 100 % therapeutic effect after 3 days, compared with only 62.21 % for ivermectin. Enzyme assays showed that M. anisopliae significantly upregulated activities of GST, SOD and CAT in Psoroptes mites. The results indicate that M. anisopliae may be an effective biological agent for control of P. ovis var. cuniculi infestations in rabbits and the acaricidal activity may be associated with the changes of enzyme activities of the detoxification and antioxidant system in Psoroptes mites.

2.
Parasit Vectors ; 13(1): 86, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-32070412

RESUMO

An aberrant Ascaris suum infection in a domestic dog in China in 2019 is described for the first time. This pathogen is a common roundworm of pigs with few reported cases in domestic animals. Our findings suggest a wider infection range with a possible transmission of A. suum to domestic animals that interact with humans.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31912322

RESUMO

Development and application of advanced mechanical models of soft tissues and their growth represent one of the main directions in modern mechanics of solids. Such models are increasingly used to deal with complex biomedical problems. Prediction of in-stent restenosis for patients treated with coronary stents remains a highly challenging task. Using a finite element method, this paper presents a mechanistic approach to evaluate the development of in-stent restenosis in an artery following stent implantation. Hyperelastic models with damage, verified with experimental results, are used to describe the level of tissue damage in arterial layers and plaque caused by such intervention. A tissue-growth model, associated with vessel damage, is adopted to describe the growth behaviour of a media layer after stent implantation. Narrowing of lumen diameter with time is used to quantify the development of in-stent restenosis in the vessel after stenting. It is demonstrated that stent designs and materials strongly affect the stenting-induced damage in the media layer and the subsequent development of in-stent restenosis. The larger the artery expansion achieved during balloon inflation, the higher the damage introduced to the media layer, leading to an increased level of in-stent restenosis. In addition, the development of in-stent restenosis is directly correlated with the artery expansion during the stent deployment. The correlation is further used to predict the effect of a complex clinical procedure, such as stent overlapping, on the level of in-stent restenosis developed after percutaneous coronary intervention.

4.
Mol Ther ; 28(2): 422-430, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-31843447

RESUMO

Short hairpin RNAs that are delivered by recombinant adeno-associated virus (rAAV) have the potential to elicit long-term RNAi therapy for human disease. However, the discovery that short hairpin sequences can cause truncation of the rAAV genome calls into question the efficiency and gene-silencing specificity of this strategy in humans. Here, we report that embedding the guide strand of a small silencing RNA into an artificial microRNA (miRNA) scaffold derived from mouse miRNA-33 ensures rAAV genomic integrity and reduces off-targeting by 10-fold, while maintaining effective in vivo target gene repression in mice.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31795105

RESUMO

Water resource security is an important condition for socio-economic development. Recently, the process of urbanization brings increasing pressures on water resources. Thus, a good understanding of harmonious development of urbanization and water resource security (WRS) systems is necessary. This paper examined the coordination state between urbanization and WRS and its obstacle factors in Beijing city, utilizing the improved coupling coordination degree (ICCD) model, obstacle degree model, and indicator data from 2008 to 2017. Results indicated that: (1) The coupling coordination degree between WRS and urbanization displayed an overall upward tendency during the 2008-2017 period; the coupling coordination state has changed from an imbalanced state into a good coordination state, experiencing from a high-speed development stage (2008-2010), through a steady growth stage (2010-2014), towards a low-speed growth (2014-2017). (2) In urbanization system, both the social and spatial urbanizations have the greatest obstruction to the development of urbanization-WRS system. The subsystems of pressure and state are the domain obstacle subsystems in WRS system. These results can provide important support for urban planning and water resource protection in the future, and hold great significance for urban sustainable development.

6.
Artigo em Inglês | MEDLINE | ID: mdl-31880541

RESUMO

Near infrared-visible (NIR-VIS) heterogeneous face recognition refers to the process of matching NIR to VIS face images. Due to self-occlusion and sensing gap, NIR face images lose some visible lighting contents so that they are always incomplete compared to VIS face images. This paper models high resolution heterogeneous face synthesis as a complementary combination of two components, a texture inpainting component and pose correction component. The inpainting component synthesizes and inpaints VIS image textures from NIR image textures. The correction component maps any pose in NIR images to a frontal pose in VIS images, resulting in paired NIR and VIS textures. A warping procedure is developed to integrate the two components into an end-to-end deep network. A fine-grained discriminator and a wavelet-based discriminator are designed to supervise intra-class variance and visual quality respectively. One 3D-based pose correction loss, two adversarial losses and one pixel loss are imposed to ensure synthesis results. We demonstrate that by attaching the correction component, we can simplify heterogeneous face synthesis from one-to-many unpaired image translation to one-to-one paired image translation. Experimental results show that our network not only generates high-resolution VIS face images and but also facilitates the accuracy improvement of heterogeneous face recognition.

7.
Parasit Vectors ; 12(1): 587, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842981

RESUMO

BACKGROUND: Mites of the genus Chorioptes are non-burrowing and cause mange in a wide range of domestic and wild animals including cattle, horses, sheep, goats, panda, moose, camelids, mydaus and alpacas. Molecular biology and host-parasite interactions of Chorioptes texanus are poorly understood, and only a few C. texanus genes and transcript sequences are available in public databases including the allergen genes. METHODS: Chorioptes texanus RNA was isolated from mites, and the transcriptome of C. texanus was analyzed using bioinformatics tools. Chorioptes texanus unigenes were compared with the allergen protein sequences from the mite allergen database website to predict the potential allergens. Chorioptes texanus putative allergen unigenes were compared with hydrolase genes by building a C. texanus hydrolase gene library with the best match of the homologous sequences. Three allergen genes were cloned and expressed, their recombinant proteins were purified and their allergenic activities were preliminarily investigated. RESULTS: Transcriptome sequencing (RNA-Seq) of C. texanus was analyzed and results demonstrated that 33,138 unigenes were assembled with an average length of 751 bp. A total of 15,130 unigenes were annotated and 5598 unigenes were enriched in 262 KEGG signaling pathways. We obtained 209 putative allergen genes and 34 putative allergen-hydrolase genes. Three recombinant allergen proteins were observed to induce different degrees of allergic reactions on rabbit skin. CONCLUSIONS: The present transcriptome data provide a useful basis for understanding the host-parasite interaction and molecular biology of the C. texanus mite. The allergenic activities of recombinant Euroglyphus maynei 1-like (Eur m 1-like) protein, Dermatophagoides ptreronyssinus 1-like (Der p 1-like) protein and Dermatophagoides ptreronyssinus 7-like (Der p 7-like) protein were preliminarily investigated by intradermal skin test. Meanwhile, differences in eosinophil counts were observed in different injected sites of the skin. The identification of putative allergen genes and hydrolase genes offers opportunities for the development of new diagnostic, prevention and treatment methods.

8.
Cell Mol Immunol ; 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31541182

RESUMO

Mechanistic target of rapamycin complex 1 (mTORC1) regulates CD8+ T-cell differentiation and function. Despite the links between PI3K-AKT and mTORC1 activation in CD8+ T cells, the molecular mechanism underlying mTORC1 activation remains unclear. Here, we show that both the kinase activity and the death domain of DAPK1 are required for maximal mTOR activation and CD8+ T-cell function. We found that TCR-induced activation of calcineurin activates DAPK1, which subsequently interacts with TSC2 via its death domain and phosphorylates TSC2 to mediate mTORC1 activation. Furthermore, both the kinase domain and death domain of DAPK1 are required for CD8+ T-cell antiviral responses in an LCMV infection model. Together, our data reveal a novel mechanism of mTORC1 activation that mediates optimal CD8+ T-cell function and antiviral activity.

9.
Biomicrofluidics ; 13(4): 044108, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31372195

RESUMO

Two-dimensional (2D) cell culture is not ideal for traditional drug screening, because 2D culture does not accurately mimic the physiological microenvironment of tumor cells. Thus, a drug-screening system which more closely mimics the microenvironment of in vivo tumors is necessary. Here, we present a biomimicking bilayer microfluidic device that can facilitate antitumor drug screening. The microfluidic device consists of two polydimethylsiloxane (PDMS) pieces with channels which are separated by a semipermeable membrane to allow water, oxygen, and nutrition supply, while preventing cell migration. The channels embedded on the two PDMS pieces overlap each other over a long distance to ensure a larger exchange area to mimic the blood vessel-tumor model. High concentrations of endothelial cells (EC) are first seeded onto the membrane through the apical channel, and after a two-day culture, a confluent EC monolayer forms. Tumor spheroid-laden Matrigel is then seeded into the basal channel. After the Matrigel is cured, the device is ready for drug testing. Paclitaxel is used as the model drug for testing. Confocal microscopy and ImageJ are used to assess the efficacy of different concentrations of paclitaxel, and optical coherence tomography (OCT) is employed to determine the tumor volumetric change after the drug treatment. The results indicate that the proposed bilayer microfluidic device in combination with confocal and OCT optical characterization provide an efficient platform for antitumor drug testing.

10.
Hum Gene Ther ; 30(8): 946-956, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31072208

RESUMO

Recombinant adeno-associated viruses (rAAVs) have become favorable gene delivery vehicles for expressing therapeutic transgenes. Capsid engineering efforts to produce novel AAVs with improved transduction efficiencies, unique tissue specificities, and reduced host immunities are a direct response to the high demand for treatment needs that preexisting rAAVs cannot currently fulfill. New AAV capsids discovered by directed evolution methods, in silico design, or from natural proviral sequences ultimately require extensive characterization in relevant in vivo models. Consequently, quantitative screening of candidate capsid libraries now requires reliable high-throughput sequencing approaches. In this study, we have developed a vector/transgene tracking system that employs the indexing of a non-coding RNA. Specifically, a barcoded Tough Decoy (bcTuD) that express highly stable RNA transcripts that can be used as readouts for transduction efficiency. The pseudo-hairpin structure of the bcTuD contains a variable region that is amenable to barcode insertion, which can be detected by target amplicon sequencing. The described approach, named AAV-bcTuD screening, offers a new alternative for in vivo assessment of rAAV that can accurately quantify vector genomes and transcript abundances in tissues, as exampled by the demonstration in liver and brain infections. Proof-of-concept is provided to show that vector genome and transcript detection in tissues with this method is accurate and consistent for a vector dose range of upwards to four logs in a mixed vector injection, showing that this technique is robust, sensitive, and applicable for multiplexed screening of capsid performance in vivo.

11.
Front Immunol ; 10: 606, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30984183

RESUMO

Follicular helper T cells (TFH cells), known as the primary "helpers" of the germinal center (GC) reaction, promote the humoral immune response to defend against various pathogens. Under conditions of infection by different types of pathogens, many shared transcription factors (TFs), such as Bcl-6, TCF-1, and Maf, are selectively enriched in pathogen-specific TFH cells, orchestrating TFH cell differentiation and function. In addition, TFH cells also coexpress environmentally associated TFs as their conventional T cell counterparts (such as T-bet, GATA-3, or ROR-γt, which are expressed in Th1, Th2, or Th17 cells, respectively). These features likely indicate both the lineage-specificity and environmental adaption of the TFH cell responses. However, the extent to which the TFH cell response relies on these environmentally specific TFs is not completely understood. Here, we found that T-bet was specifically expressed in Type I TFH cells but not Type II TFH cells. While dispensable for the early fate commitment of TFH cells, T-bet was essential for the maintenance of differentiated TFH cells, promoting their proliferation, and inhibiting their apoptosis during acute viral infection. Microarray analysis showed both similarities and differences in transcriptome dependency on T-bet in TFH and TH1 cells, suggesting the distinctive role of T-bet in TFH cells. Collectively, our findings reveal an important and specific supporting role for T-bet in type I TFH cell response, which can help us gain a deeper understanding of TFH cell subsets.

12.
Front Immunol ; 10: 169, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30814995

RESUMO

The long-term persistence of viral antigens drives virus-specific CD8 T cell exhaustion during chronic viral infection. Yet exhausted, CD8 T cells are still endowed with certain levels of effector function, by which they can keep viral replication in check in chronic infection. However, the regulatory factors involved in regulating the effector function of exhausted CD8 T cell are largely unknown. Using mouse model of chronic LCMV infection, we found that the deletion of transcription factor TCF-1 in LCMV-specific exhausted CD8 T cells led to the profound reduction in cytokine production and degranulation. Conversely, ectopic expression of TCF-1 or using agonist to activate TCF-1 activities promotes the effector function of exhausted CD8 T cells. Mechanistically, TCF-1 fuels the functionalities of exhausted CD8 T cells by promoting the expression of an array of key effector function-associated transcription regulators, including Foxo1, Zeb2, Id3, and Eomes. These results collectively indicate that targeting TCF-1 mediated transcriptional pathway may represent a promising immunotherapy strategy against chronic viral infections by reinvigorating the effector function of exhausted virus-specific CD8 T cells.


Assuntos
Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/metabolismo , Fator 1 de Transcrição de Linfócitos T/metabolismo , Viroses/etiologia , Viroses/metabolismo , Transferência Adotiva , Animais , Sobrevivência Celular , Doença Crônica , Citocinas/metabolismo , Modelos Animais de Doenças , Expressão Gênica , Coriomeningite Linfocítica/imunologia , Coriomeningite Linfocítica/metabolismo , Coriomeningite Linfocítica/terapia , Coriomeningite Linfocítica/virologia , Vírus da Coriomeningite Linfocítica/imunologia , Camundongos , Camundongos Transgênicos , Fator 1 de Transcrição de Linfócitos T/genética , Quimeras de Transplante , Carga Viral , Viroses/terapia
13.
Cell Mol Immunol ; 2019 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-30842630

RESUMO

Epigenetic modifications to histones dictate the differentiation of naïve CD4+ T cells into different subsets of effector T helper (TH) cells. The histone methyltransferase enhancer of zeste homolog 2 (EZH2) has been implicated in the mechanism regulating the differentiation of TH1, TH2 and regulatory T (Treg) cells. However, whether and how EZH2 regulates follicular helper T (TFH) cell differentiation remain unknown. Using a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection, we observed abundant EZH2 expression and associated H3K27me3 modifications preferentially in the early committed virus-specific TFH cells compared to those in TH1 cells. Ablation of EZH2 in LCMV-specific CD4+ T cells leads to a selective impairment of early TFH cell fate commitment, but not late TFH differentiation or memory TFH maintenance. Mechanistically, EZH2 specifically stabilizes the chromatin accessibility of a cluster of genes that are important for TFH fate commitment, particularly B cell lymphoma 6 (Bcl6), and thus directs TFH cell commitment. Therefore, we identified the chromatin-modifying enzyme EZH2 as a novel regulator of early TFH differentiation during acute viral infection.

14.
Artigo em Inglês | MEDLINE | ID: mdl-30736007

RESUMO

Nowadays, video quality assessment (VQA) is essential to video compression technology applied to video transmission and storage. However, small-scale video quality databases with imbalanced samples and low-level feature representations for distorted videos impede the development of VQA methods. In this paper, we propose a full-reference (FR) VQA metric integrating transfer learning with a convolutional neural network (CNN). First, we imitate the feature-based transfer learning framework to transfer the distorted images as the related domain, which enriches the distorted samples. Second, to extract high-level spatiotemporal features of the distorted videos, a six-layer CNN with the acknowledged learning ability is pretrained and finetuned by the common features of the distorted image blocks (IBs) and video blocks (VBs), respectively. Notably, the labels of the distorted IBs and VBs are predicted by the classic FR metrics. Finally, based on saliency maps and the entropy function, we conduct a pooling stage to obtain the quality scores of the distorted videos by weighting the block-level scores predicted by the trained CNN. In particular, we introduce a preprocessing and a postprocessing to reduce the impact of inaccurate labels predicted by the FR-VQA metric. Due to feature learning in the proposed framework, two kinds of experimental schemes including train-test iterative procedures on one database and tests on one database with training other databases are carried out. The experimental results demonstrate that the proposed method has high expansibility and is on a par with some state-of-the-art VQA metrics on two widely used VQA databases with various compression distortions.

15.
Nat Commun ; 10(1): 270, 2019 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-30655512

RESUMO

Discovery of thermoelectric materials has long been realized by the Edisonian trial and error approach. However, recent progress in theoretical calculations, including the ability to predict structures of unknown phases along with their thermodynamic stability and functional properties, has enabled the so-called inverse design approach. Compared to the traditional materials discovery, the inverse design approach has the potential to substantially reduce the experimental efforts needed to identify promising compounds with target functionalities. By adopting this approach, here we have discovered several unreported half-Heusler compounds. Among them, the p-type TaFeSb-based half-Heusler demonstrates a record high ZT of ~1.52 at 973 K. Additionally, an ultrahigh average ZT of ~0.93 between 300 and 973 K is achieved. Such an extraordinary thermoelectric performance is further verified by the heat-to-electricity conversion efficiency measurement and a high efficiency of ~11.4% is obtained. Our work demonstrates that the TaFeSb-based half-Heuslers are highly promising for thermoelectric power generation.

16.
IEEE Trans Pattern Anal Mach Intell ; 41(5): 1027-1042, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-29993436

RESUMO

Unsupervised domain adaptation aims to leverage the labeled source data to learn with the unlabeled target data. Previous trandusctive methods tackle it by iteratively seeking a low-dimensional projection to extract the invariant features and obtaining the pseudo target labels via building a classifier on source data. However, they merely concentrate on minimizing the cross-domain distribution divergence, while ignoring the intra-domain structure especially for the target domain. Even after projection, possible risk factors like imbalanced data distribution may still hinder the performance of target label inference. In this paper, we propose a simple yet effective domain-invariant projection ensemble approach to tackle these two issues together. Specifically, we seek the optimal projection via a novel relaxed domain-irrelevant clustering-promoting term that jointly bridges the cross-domain semantic gap and increases the intra-class compactness in both domains. To further enhance the target label inference, we first develop a 'sampling-and-fusion' framework, under which multiple projections are independently learned based on various randomized coupled domain subsets. Subsequently, aggregating models such as majority voting are utilized to leverage multiple projections and classify unlabeled target data. Extensive experimental results on six visual benchmarks including object, face, and digit images, demonstrate that the proposed methods gain remarkable margins over state-of-the-art unsupervised domain adaptation methods.

17.
IEEE Trans Pattern Anal Mach Intell ; 41(7): 1761-1773, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-29993534

RESUMO

Heterogeneous face recognition (HFR) aims at matching facial images acquired from different sensing modalities with mission-critical applications in forensics, security and commercial sectors. However, HFR presents more challenging issues than traditional face recognition because of the large intra-class variation among heterogeneous face images and the limited availability of training samples of cross-modality face image pairs. This paper proposes the novel Wasserstein convolutional neural network (WCNN) approach for learning invariant features between near-infrared (NIR) and visual (VIS) face images (i.e., NIR-VIS face recognition). The low-level layers of the WCNN are trained with widely available face images in the VIS spectrum, and the high-level layer is divided into three parts: the NIR layer, the VIS layer and the NIR-VIS shared layer. The first two layers aim at learning modality-specific features, and the NIR-VIS shared layer is designed to learn a modality-invariant feature subspace. The Wasserstein distance is introduced into the NIR-VIS shared layer to measure the dissimilarity between heterogeneous feature distributions. W-CNN learning is performed to minimize the Wasserstein distance between the NIR distribution and the VIS distribution for invariant deep feature representations of heterogeneous face images. To avoid the over-fitting problem on small-scale heterogeneous face data, a correlation prior is introduced on the fully-connected WCNN layers to reduce the size of the parameter space. This prior is implemented by a low-rank constraint in an end-to-end network. The joint formulation leads to an alternating minimization for deep feature representation at the training stage and an efficient computation for heterogeneous data at the testing stage. Extensive experiments using three challenging NIR-VIS face recognition databases demonstrate the superiority of the WCNN method over state-of-the-art methods.


Assuntos
Identificação Biométrica/métodos , Face/anatomia & histologia , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Algoritmos , Bases de Dados Factuais , Expressão Facial , Humanos , Processamento de Imagem Assistida por Computador/métodos
18.
ACS Appl Mater Interfaces ; 11(1): 511-516, 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30525424

RESUMO

Phonon scattering through alloying is a highly effective way to reduce lattice thermal conductivity due to the mass difference between the host and alloyed atoms and strains caused by the different atoms. In this work we investigate the thermoelectric properties of Te between 323 and 623 K. By varying the alloying concentration of Se, a minimum lattice thermal conductivity was achieved with ∼10% (by stoichiometry) alloying of Te by Se. Additionally, Sb has been used as a dopant to increase the carrier concentration of the system. With reduced lattice thermal conductivity by Se alloying and increased carrier concentration by Sb doping, the room-temperature figure of merit ( ZT) increased by 60%, leading to an average ZT of ∼0.8 in Te0.88Se0.10Sb0.02, which corresponds to an engineering figure of merit ( ZT)eng ∼ 0.5 between 323 and 623 K and an efficiency of ∼8% in the same temperature range. The results indicate that the combination of Se alloying and Sb doping is successful in improving the thermoelectric properties of Te.

19.
Mol Ther Methods Clin Dev ; 11: 65-72, 2018 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-30397628

RESUMO

Pre-existing neutralizing antibody (NAb) against adeno-associated virus (AAV) commonly found in primates is a major host barrier that can severely compromise in vivo gene transfer by AAV vectors. To achieve proof-of-concept success in clinical development of recombinant AAV (rAAV)-based in vivo gene therapy, it is crucial to consider the potential interference of NAb and to enroll serologically compatible study subjects. In this study, we report a large AAV NAb dataset comprising multiple large animal species and AAV serotypes and compare two NAb assays based on in vitro or in vivo transduction inhibition, respectively. Together with previously published AAV seroepidemiology studies, these data can serve as a reference for selecting suitable serotypes, study subjects of large animal species, and potentially human patients for rAAV treatment. In addition, we modeled the intrathalamus rAAV9 delivery in the presence of circulating anti-AAV9 NAb generated by either pre-immunization or passive transfer of NAb-positive large animal serum to mice. The data showed that circulating NAb may not be the sole determinant to inhibit brain transduction. Other aspects of pre-existing AAV immunity following natural infection or rAAV administration may be further studied to establish a more accurate inclusion criterion for clinical studies employing intraparenchymal rAAV9 injections.

20.
Parasit Vectors ; 11(1): 599, 2018 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-30454025

RESUMO

BACKGROUND: Scabies is caused by Sarcoptes scabiei burrowing into the stratum corneum of the host's skin and is detrimental to the health of humans and animals. Vaccines are an attractive alternative to replace the acaricides currently used in their control. METHODS: In the present study, the S. scabiei chitinase-like protein 5 (SsCLP5) was characterized and recombinant SsCLP5 (rSsCLP5) was evaluated as a candidate vaccine protein for anti-mite protection in rabbits. The expression, characterization and immunolocalization of SsCLP5 were examined. Vaccination experiments were performed on three test groups (n = 12 per group) immunized with purified rSsCLP5. Control groups (n = 12 per group) were immunized with PBS, QuilA saponin or empty vector protein. After challenge, the inflammatory reaction and skin lesions were graded and rSsCLP5 indirect ELISA was used to detect antibody IgG levels in serum samples at the time of vaccination and post-challenge. RESULTS: The results showed that rSsCLP5 had high immunoreactivity and immunogenicity. In S. scabiei, SsCLP5 had a wide distribution in the chewing mouthpart, legs and exoskeleton, especially the outer layer of the exoskeleton. Vaccination with rSsCLP5 resulted in 74.3% (26/35) of rabbits showing no detectable lesions after challenge with S. scabiei. CONCLUSIONS: Our data demonstrate that rSsCLP5 is a promising candidate for a recombinant protein-based vaccine against S. scabiei. This study also provides a method for studying scabies vaccine using rabbit as an animal model and a basis for screening more effective candidate proteins.


Assuntos
Quitinases/imunologia , Coelhos/parasitologia , Sarcoptes scabiei/imunologia , Escabiose/veterinária , Vacinas/imunologia , Animais , Quitinases/administração & dosagem , Quitinases/química , Quitinases/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Imunogenicidade da Vacina , Imunoglobulina G/sangue , Masculino , Distribuição Aleatória , Proteínas Recombinantes/imunologia , Sarcoptes scabiei/química , Sarcoptes scabiei/enzimologia , Escabiose/imunologia , Escabiose/parasitologia , Escabiose/prevenção & controle , Pele/efeitos dos fármacos , Pele/parasitologia , Vacinação/veterinária , Vacinas/administração & dosagem
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