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1.
J Phys Chem A ; 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35679610

RESUMO

Accurate and efficient simulation of liquids, such as water and salt solutions, using high-level wave function theories is still a formidable task for computational chemists owing to the high computational costs. In this study, we develop a deep machine learning potential based on fragment-based second-order Møller-Plesset perturbation theory (DP-MP2) for water through neural networks. We show that the DP-MP2 potential predicts the structural, dynamical, and thermodynamic properties of liquid water in better agreement with the experimental data than previous studies based on density functional theory (DFT). The nuclear quantum effects (NQEs) on the properties of liquid water are also examined, which are noticeable in affecting the structural and dynamical properties of liquid water under ambient conditions. This work provides a general framework for quantitative predictions of the properties of condensed-phase systems with the accuracy of high-level wave function theory while achieving significant computational savings compared to ab initio simulations.

2.
Nucleic Acids Res ; 2022 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-35687106

RESUMO

ARMC5 is implicated in several pathological conditions, but its function remains unknown. We have previously identified CUL3 and RPB1 (the largest subunit of RNA polymerase II (Pol II) as potential ARMC5-interacting proteins. Here, we show that ARMC5, CUL3 and RBX1 form an active E3 ligase complex specific for RPB1. ARMC5, CUL3, and RBX1 formed an active E3 specific for RPB1. Armc5 deletion caused a significant reduction in RPB1 ubiquitination and an increase in an accumulation of RPB1, and hence an enlarged Pol II pool in normal tissues and organs. The compromised RPB1 degradation did not cause generalized Pol II stalling nor depressed transcription in the adrenal glands but did result in dysregulation of a subset of genes, with most upregulated. We found RPB1 to be highly expressed in the adrenal nodules from patients with primary bilateral macronodular adrenal hyperplasia (PBMAH) harboring germline ARMC5 mutations. Mutant ARMC5 had altered binding with RPB1. In summary, we discovered that wildtype ARMC5 was part of a novel RPB1-specific E3. ARMC5 mutations resulted in an enlarged Pol II pool, which dysregulated a subset of effector genes. Such an enlarged Pol II pool and gene dysregulation was correlated to adrenal hyperplasia in humans and KO mice.

3.
Nutrients ; 14(11)2022 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-35684007

RESUMO

Sweet tea (Lithocarpus litseifolius [Hance] Chun) is a new resource for food raw materials, with plenty of health functions. This study aimed to investigate the preventive effect and potential mechanism of sweet tea extract (STE) against ulcerative colitis (UC). Briefly, BABL/c mice were treated with STE (100 and 400 mg/kg) for 2 weeks to prevent 3% dextran sulfate sodium (DSS)-induced UC. It was found that STE supplementation significantly prevented DSS-induced UC symptoms; suppressed the levels of pro-inflammatory mediators, such as myeloperoxidase and tumor necrosis factor-α; increased the levels of anti-inflammatory cytokines; and up-regulated the expression of tight junction proteins (Zonula occludens-1 and Occludin). STE also altered the gut microbiota profile of UC mice by increasing Bacteroidetes, Lactobacillus, Akkermansia, Lachnospiraceae_NK4A136_group, and Alistipes and inhibiting Firmicutes, Proteobacteria, and Helicobacter, accompanied by a significant increase in the content of butyric acid. Moreover, STE increased the expression of G-protein-coupled receptor (GPR) 43 and GPR109A and inhibited the expression of histone deacetylase 3 (HDAC3) and nuclear factor-κB p65 (NF-κB p65) in the colon. In conclusion, this study indicated that STE has a good preventive effect on UC by regulating gut microbiota to activate butyrate-GPR-mediated anti-inflammatory signaling and simultaneously inhibit HDAC3/NF-κB inflammatory signaling.


Assuntos
Colite Ulcerativa , Colite , Microbioma Gastrointestinal , Animais , Anti-Inflamatórios/uso terapêutico , Ácido Butírico/metabolismo , Colite/induzido quimicamente , Colite/metabolismo , Colite/prevenção & controle , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/tratamento farmacológico , Colite Ulcerativa/prevenção & controle , Colo/metabolismo , Sulfato de Dextrana/efeitos adversos , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos C57BL , NF-kappa B/metabolismo , Chá/efeitos adversos
4.
J Hip Preserv Surg ; 9(1): 10-17, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-35651706

RESUMO

Load-bearing capacity of the bone structures of anterolateral weight-bearing area plays an important role in the progressive collapse in osteonecrosis of the femoral head (ONFH). The purpose of this study is to assess the efficacy of combined evaluation of anteroposterior (AP) and frog-leg lateral (FLL) view in diagnosing collapse. Between December 2016 and August 2018, a total of 478 hips from 372 patients with ONFH (268 male, 104 female; mean age 37.9 ± 11.4 years) were retrospectively evaluated. All patients received standard AP and FLL views of hip joints. Japanese Investigation Committee (JIC) classification system was used to classify necrotic lesion in AP view. Anterior necrotic lesion was evaluated by FLL view. All patients with pre-collapse ONFH underwent non-operative hip-preserving therapy. The collapse rates were calculated and compared with Kaplan-Meier survival analysis with radiological collapse as endpoints. Forty-four (44/478, 9.2%) hips were classified as type A, 65 (65/478, 13.6%) as type B, 232 (232/478, 48.5%) as type C1 and 137 (137/478, 28.7%) as type C2. Three hundred cases (300/478, 62.5%) were collapsed at the initial time point. Two hundred and twenty six (226/300, 75.3%) hips and 298 (298/300, 99.3%) hips collapse were identified with AP view and FLL view, respectively. An average follow-up of 37.0 ± 32.0 months was conducted to evaluate the occurrence of collapse in 178 pre-collapse hips. Collapses occurred in 89 hips (50.0%). Seventy-seven (77/89, 86.5%) hips were determined with AP view alone and 85 (85/89, 95.5%) hips were determined with the combination of AP and FLL views. The collapse rates at five years were reported as 0% and 0%, 16.2% and 24.3%, 58.3% and 68.1% and 100% and 100% according to AP view alone or combination of AP and FLL views for types A, B, C1 and C2, respectively. The collapse can be diagnosed more accurately by combination of AP and FLL views. Besides, JIC type A and type B ONFH can be treated with conservative hip preservation, but pre-collapse type C2 ONFH should be treated with joint-preserving surgery. Type C1 needs further study to determine which subtype has potential risk of collapse.

6.
Cell J ; 24(5): 239-244, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35717564

RESUMO

Objective: Four and a half Lin-11, Isl-1, Mac-3 (LIM) protein 1 (FHL1) is one of the FHL protein family, which is regarded as a tumor suppressor in the multiple malignant tumors. In this study, we aimed to explore the regulatory effects and mechanisms of FHL1 on lung cancer cell invasion. Materials and Methods: In this experimental study, bioinformatics analysis of FHL1 transcripts in human lung adenocarcinomas of TCGA database was performed. Quantitative real-time polymerase chain reaction (PCR) was performed to detect FHL1 mRNA expression in 15 paired human lung cancer tissues and their adjacent normal lung tissues, or lung cancer cell lines (A549 and H1299) in comparison with human bronchial epithelial cell line (Beas- 2B). Moreover, western blot was used to analyze FHL1 and rho GDP-dissociation inhibitor beta (RhoGDIß) protein expression in the indicated cell lines. Also, transwell assays were employed to measure the migrated, and invaded of indicated cell lines. Results: FHL1 transcripts were downregulated in the human lung adenocarcinoma. The impaired FHL1 transcripts were positively correlated with advanced tumor node metastasis (TNM) stage. Moreover, as compared to the adjacent normal lung tissues, FHL1 mRNA was low expressed in 15 paired human lung cancer tissues than their adjacent normal lung tissues. Besides, FHL1 mRNA and protein expression were also reduced in H1299 and A549 cell lines in comparison with Beas-2B cell line. Overexpressed FHL1 protein inhibited the invasive ability of H1299 and A549 cell lines. Mechanically, FHL1 protein overexpression increased the RhoGDIß protein and mRNA abundance, while knockdown of RhoGDIß protein, completely restored the invasion ability of A549 (Flag-FHL1) cell line. Conclusion: Our findings indicated that as a key FHL1 downstream regulator, RhoGDIß is in charge of FHL1 inhibiting lung cancer cell invasion abilities, providing a critical insight into understanding the role of FHL1 for lung cancer development.

7.
IEEE Trans Cybern ; PP2022 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-35714092

RESUMO

Fault-tolerant control (FTC) may conceal fault symptoms, thereby increasing the difficulty of fault diagnosis (FD). In this article, a novel framework for the cooperative design of active FD and FTC is proposed to optimize FD performance while maintaining fault-tolerance performance. The proposed framework consists of four steps: 1) controller design; 2) residual generation; 3) performance evaluation; and 4) gain tuning. First, a controller with undetermined gains is constructed, and a fault detection observer is designed to generate residuals that can indicate the fault. Then, the performance of fault detection is evaluated. Finally, suboptimal controller gains are obtained by solving an optimization problem. Within the framework of the collaborative design, the occurring faults can be detected faster and more accurately, and the performance of FTC can be guaranteed at the same time. A simulation study is provided to demonstrate the effectiveness of the developed framework.

8.
Gastroenterol Rep (Oxf) ; 10: goac020, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35711715

RESUMO

Background: The impact of the preoperative carbohydrate antigen 125 (CA125) level on the survival of metastatic colorectal cancer (CRC) patients undergoing primary tumor resection (PTR) remains uncertain. The aim of this study was to assess the prognostic value in overall survival (OS) and cancer-specific survival (CSS) between patients with and without an elevated preoperative CA125 level. Methods: All metastatic CRC patients receiving PTR between 2007 and 2017 at the Sixth Affiliated Hospital of Sun Yat-sen University (Guangzhou, China) were retrospectively included. OS and CSS rates were compared between patients with and without elevated preoperative CA125 levels. Results: Among 326 patients examined, 46 (14.1%) exhibited elevated preoperative CA125 levels and the remaining 280 (85.9%) had normal preoperative CA125 levels. Patients with elevated preoperative CA125 levels had lower body mass index, lower preoperative albumin level, lower proportion of preoperative chemotherapy, higher carcinoembryonic antigen and carbohydrate antigen 19-9 (CA19-9) levels, poorer differentiation, and more malignant histopathological type than patients with normal preoperative CA125 levels. In addition, patients with elevated preoperative CA125 levels exhibited more advanced pathological T and N stages, more peritoneal metastasis, and more vessel invasion than patients with normal preoperative CA125 levels. Moreover, the primary tumor was more likely to be located at the colon rather than at the rectum in patients with elevated CA125 levels. Both OS and CSS rates in patients with elevated preoperative CA125 levels were significantly lower than those in patients with normal preoperative CA125 levels. Multivariate Cox regression analysis revealed that an elevated preoperative CA125 level was significantly associated with poor prognosis in metastatic CRC patients undergoing PTR. The hazard ratio (HR) in OS was 2.36 (95% confidence interval [CI], 1.67-3.33, P < 0.001) and the HR in CSS was 2.50 (95% CI, 1.77-3.55, P < 0.001). The survival analysis stratified by peritoneal metastasis also demonstrated that patients with elevated preoperative CA125 levels had lower OS and CSS rates regardless of peritoneal metastasis. Conclusion: Based on an analysis of metastatic CRC patients undergoing PTR, an elevated preoperative CA125 level was associated with poor prognosis, which should be taken into consideration in clinical practice.

9.
J Surg Oncol ; 2022 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-35661159

RESUMO

BACKGROUND: Patients with nonmetastatic pT3-4 colon cancers are prone to develop metachronous peritoneal carcinomatosis (mPC). Risk factors for mPC and the influence of mutant kirsten rat sarcoma viral oncogene (KRAS)/neuroblastoma rat sarcoma (NRAS)/v-raf murine sarcoma viral oncogene homolog B1 (BRAF) and DNA mismatch repair (MMR) status on mPC remain to be described in these patients. METHOD: All enrolled patients were identified from the prospectively collected colorectal cancer database of a tertiary referral hospital between 2013 and 2018. Multivariate analysis was used to identify risk factors associated with mPC. RESULTS: Of the 1689 patients with nonmetastatic pT3-4 colon carcinoma, 8.4% (142/1689) progressed to mPC. Endoscopic obstruction (HR = 3.044, p < 0.001), elevated CA125 (HR = 1.795, p = 0.009), pT (T4a vs. T3, HR = 2.745, p < 0.001; T4b vs. T3, HR = 3.167, p = 0.001), pN (N1 vs. N0, HR = 2.592, p < 0.001; N2 vs. N0, HR = 4.049, p < 0.001), less than 12 lymph nodes harvested (HR = 2.588, p < 0.001), mucinous or signet ring cell carcinoma (HR = 1.648, p = 0.038), perineural invasion (HR = 1.984, p < 0.001), and adjuvant chemotherapy (HR = 1.522, p = 0.039) were strongly related to mPC but that mutant KRAS/NRAS/BRAF and MMR status was not associated with mPC. CONCLUSION: This study identified the high-risk factors for mPC in patients with nonmetastatic pT3-4 colon carcinoma, and these factors should be considered in selective preventive therapy and close follow-up for patients subsequently deemed to have high risk for mPC.

10.
Front Mol Biosci ; 9: 888721, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35677881

RESUMO

Aromatic aldehydes are important industrial raw materials mainly synthesized by anti-Markovnikov (AM) oxidation of corresponding aromatic olefins. The AM product selectivity remains a big challenge. P450 aMOx is the first reported enzyme that could catalyze AM oxidation of aromatic olefins. Here, we reported a rational design strategy based on the "butterfly" model of the active site of P450 aMOx. Constrained molecular dynamic simulations and a binding energy analysis of key residuals combined with an experimental alanine scan were applied. As a result, the mutant A275G showed high AM selectivity of >99%. The results also proved that the "butterfly" model is an effective design strategy for enzymes.

11.
Mol Psychiatry ; 2022 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-35701596

RESUMO

Although sleep, physical activity and sedentary behavior have been found to be associated with dementia risk, findings are inconsistent and their joint relationship remains unclear. This study aimed to investigate independent and joint associations of these three modifiable behaviors with dementia risks. A total of 431,924 participants (median follow-up 9.0 years) without dementia from UK Biobank were included. Multiple Cox regressions were used to estimate adjusted hazard ratios (HRs) and 95% confidence intervals (CIs). Models fitted with restricted cubic spline were conducted to test for linear and nonlinear shapes of each association. Sleep duration, leisure-time physical activity (LTPA), and screen-based sedentary behavior individually associated with dementia risks in different non-linear patterns. Sleep duration associated with dementia in a U-shape with a nadir at 7 h/day. LTPA revealed a curvilinear relationship with dementia in diminishing tendency, while sedentary behavior revealed a J-shaped relationship. The dementia risk was 17% lower in the high LTPA group (HR[95%CI]: 0.83[0.76-0.91]) and 22% higher in the high sedentary behavior group (1.22[1.10-1.35]) compared to the corresponding low-level group, respectively. A combination of seven-hour/day sleep, moderate-to-high LTPA, and low-to-moderate sedentary behavior showed the lowest dementia risk (0.59[0.50-0.69]) compared to the referent group (longer or shorter sleep/low LTPA/high sedentary behavior). Notably, each behavior was non-linearly associated with brain structures in a pattern similar to its association with dementia, suggesting they may affect dementia risk by affecting brain structures. Our findings highlight the potential to change these three daily behaviors individually and simultaneously to reduce the risk of dementia.

12.
ACS Sens ; 7(5): 1524-1532, 2022 05 27.
Artigo em Inglês | MEDLINE | ID: mdl-35512281

RESUMO

Emerging liquid biopsy methods for investigating biomarkers in bodily fluids such as blood, saliva, or urine can be used to perform noninvasive cancer detection. However, the complexity and heterogeneity of exosomes require improved methods to achieve the desired sensitivity and accuracy. Herein, we report our study on developing a breast cancer liquid biopsy system, including a fluorescence sensor array and deep learning (DL) tool AggMapNet. In particular, we used a 12-unit sensor array composed of conjugated polyelectrolytes, fluorophore-labeled peptides, and monosaccharides or glycans to collect fluorescence signals from cells and exosomes. Linear discriminant analysis (LDA) processed the fluorescence spectral data of cells and cell-derived exosomes, demonstrating successful discrimination between normal and different cancerous cells and 100% accurate classification of different BC cells. For heterogeneous plasma-derived exosome analysis, CNN-based DL tool AggMapNet was applied to transform the unordered fluorescence spectra into feature maps (Fmaps), which gave a straightforward visual demonstration of the difference between healthy donors and BC patients with 100% prediction accuracy. Our work indicates that our fluorescent sensor array and DL model can be used as a promising noninvasive method for BC diagnosis.


Assuntos
Neoplasias da Mama , Aprendizado Profundo , Exossomos , Feminino , Corantes Fluorescentes , Humanos , Biópsia Líquida/métodos
13.
Artigo em Inglês | MEDLINE | ID: mdl-35564521

RESUMO

Assessing the bacteria pathogens in the lakes with reclaimed water as major influents are important for public health. This study investigated microbial communities of five landscape lakes replenished by reclaimed water, then analyzed driven factors and identified health effects of bacterial pathogens. 16S rRNA gene sequence analysis demonstrated that Proteobacteria, Actinobacteria, Cyanobacteria, Firmicutes, and Verrucomicrobia were the most dominant phyla in five landscape lakes. The microbial community diversities were higher in June and July than that in other months. Temperature, total nitrogen and phosphorus were the main drivers of the dominant microbial from the Redundancy analysis (RDA) results. Various potential bacterial pathogens were identified, including Pseudomonas, GKS98_freshwater_group, Sporosarcina, Pseudochrobactrum, Streptomyces and Bacillus, etc, some of which are easily infectious to human. The microbial network analysis showed that some potential pathogens were nodes that had significant health effects. The work provides a basis for understanding the microbial community dynamics and safety issues for health effects in landscape lakes replenished by reclaimed water.


Assuntos
Cianobactérias , Microbiota , Pequim , China , Humanos , Lagos/microbiologia , RNA Ribossômico 16S/genética , Água
14.
Langmuir ; 38(21): 6720-6730, 2022 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-35584361

RESUMO

Water flow in a nanoscale channel is known to be affected by strong water-wall interactions; as a result, the flow considerably deviates from the conventional continuum flow. Nanochannel with a sudden contraction or expansion is the most fundamental morphological nanostructure in many nanoporous systems such as shale matrix, mudrock, membrane, etc. However, the nanoconfinement effects of water flow in nanoporous systems and its effect on macroscopic flow behavior are still evolving research topics. In this work, our recently developed pore-scale lattice Boltzmann method (LBM) considering the nanoscale effects is extended to directly simulate water flow in nanoporous systems. The results show that the flow rate is dramatically decreased in hydrophobic nanopores because of additional flow resistances at the contraction and expansion junctions. This indicates that the bundle of capillary models or the permeability averaging method overestimates the water flow rate in nanoporous media if the contraction/expansion effects between different nanopores are ignored. This work highlights the importance of wettability of the nanochannel in the determination of dynamic water flow behaviors in the contraction/expansion nanosystem. Other important aspects, including velocity distribution, flow patterns, and vortex characteristics as well as pressure variation along the flow direction, are for the first time revealed and quantified. Large differences can be found comparing gas or larger-scale water flows through the same system. A new type of pressure variation curve along the axis of flow direction is found in the hydrophobic nanochannel with a sudden contraction/expansion. This work provides the fundamental understanding of water transport through the nanoscale system with contraction and expansion effects, giving implications to a wide range of industry applications.

15.
Chem Commun (Camb) ; 58(46): 6657-6660, 2022 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-35593312

RESUMO

Herein, we designed and developed a single two-photon ratiometric fluorescence probe (TMF2P) for selective and accurate determination of mitochondrial MAO-A in live neurons. It was discovered that the increases in MAO-A levels under oxidative stress resulted in an elevated influx of Ca2+ flow into mitochondria through the transient receptor potential melastatin 2 (TRPM2) channels.


Assuntos
Mitocôndrias , Neurônios , Mitocôndrias/metabolismo , Monoaminoxidase/metabolismo , Neurônios/metabolismo , Imagem Óptica , Estresse Oxidativo
16.
J Ethnopharmacol ; 294: 115353, 2022 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-35533911

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Marsdenia tenacissima is a medicinal plant, used as a raw material for cancer treatment in China. In our previous studies, 11α-O-2-methylbutanoyl-12ß-O-tigloyl-tenacigenin B (MT2), the main steroid aglycone isolated from M. tenacissima, was found to significantly enhance the antitumor activity of paclitaxel (PTX) in vivo. However, it is unclear whether MT2 reverses multidrug resistance (MDR) in tumors. AIM OF THE STUDY: To determine the role and mechanism of MT2 in reversing tumor MDR. MATERIALS AND METHODS: MDR cell line HeLa/Tax was established from the human cervical carcinoma cell line HeLa by long-term exposure to subtoxic concentrations of PTX and was used to evaluate the ability of MT2 to restore chemosensitivity of cells both in vitro and in a nude mouse model. The expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein 2 (MRP2) was determined using western blotting and immunohistochemistry. The substrate transport function was assessed using an MDR function assay kit. The binding modes of MT2 and P-gp were determined using the conformation-sensitive anti-P-gp antibodies. The permeability and transport properties of MT2 were analyzed in Caco-2 cell monolayers. RESULTS: Compared to parental cells, HeLa/Tax cells overexpress P-gp and MRP2 and are approximately 100-360 fold more resistant to the anticancer drugs PTX, docetaxel, and vinblastine. MT2 at 5 or 10 µmol/L significantly increased the sensitivity of HeLa/Tax to these three anticancer drugs (18-56-fold decrease in IC50 value) and suppressed the expression of P-gp and MRP2. Knockdown of P-gp with small interfering RNA partially reversed MT2-induced sensitivity to PTX in HeLa/Tax cells. Moreover, MT2 directly inhibited P-gp-mediated substrate transport while interacting with membrane P-gp in non-substrate ways. MT2 was highly permeable and could not be transported in the Caco-2 cell monolayers. In nude mice bearing HeLa/Tax xenografts, the combination treatment with MT2 and PTX exerted a synergistic inhibitory effect on the growth of tumors and the expression of P-gp and MRP2 without increasing toxicity. CONCLUSION: MT2 is a potential agent for reversing MDR. It impedes membrane drug efflux pumps by suppressing P-gp and MRP2 expression, and directly inhibiting the transport function of P-gp.


Assuntos
Antineoplásicos , Marsdenia , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Animais , Antineoplásicos/farmacologia , Células CACO-2 , Resistência a Múltiplos Medicamentos , Resistencia a Medicamentos Antineoplásicos , Ésteres , Humanos , Marsdenia/química , Camundongos , Camundongos Nus , Paclitaxel/farmacologia , Esteroides/química
17.
Environ Res ; 212(Pt D): 113490, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35594958

RESUMO

Electro-dewatering technology shows a good application prospect because of its high efficiency in removing water from sludge and low energy consumption, but the potential mechanisms of sludge electro-dewatering have not been investigated in depth, which seriously limits the further development and application of electro-dewatering technology. In this study, the effects of voltage and pressure on sludge electro-dewatering performance, physicochemical characteristics and extracellular polymeric substances (EPS) compositions and distributions were investigated. The spatial distributions of EPS main components, including polysaccharide (PS) and protein (PN), were characterized by a confocal laser scanning microscopy (CLSM). The experimental results showed that under the conditions of a voltage of 40 V and a pressure of 90 kPa, the moisture content of sludge was reduced from 83.15% to 53.12%, and the bound water content of sludge in the anode layer, middle layer and cathode layer were decreased significantly from 1.16 g/g dry solid (DS) to 0.20, 0.47 and 0.35 g/g DS, respectively. The PN content of EPS in anode layer was significantly lower than that in cathode layer due to the electrochemical oxidation, while the variation of PS content showed the opposite trend, which agreed with the results visualized by CLSM. Pearson's correlation coefficient and hierarchical cluster analysis revealed that PN in TB-EPS was the major factor influencing the effect of sludge electro-dewatering. This work can be helpful to understand the potential mechanisms of electro-dewatering and provide theoretical support for the further popularization and application of electro-dewatering technology.


Assuntos
Matriz Extracelular de Substâncias Poliméricas , Esgotos , Eletrodos , Matriz Extracelular de Substâncias Poliméricas/química , Proteínas , Esgotos/química , Eliminação de Resíduos Líquidos/métodos , Águas Residuárias/análise , Água/química
18.
Food Chem ; 391: 133255, 2022 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-35609464

RESUMO

Intramolecular copigmentation, a natural phenomenon occurring widely in acylated anthocyanins, can stabilize anthocyanins to a great extent and produce color changes. In this study, the mechanism underlying the intramolecular copigmentation of malvidin-3-O-(6-O-p-coumaryl)-glucoside (MvCG) was systematically elucidated using experimental and theoretical approaches. Color analysis by UV-vis spectroscopy revealed an obvious bathochromic shift for MvCG compared to its non-acylated form, as well as higher thermostability of the former. Two dimensional NOESY spectra indicated close proximity between the coumaryl group and anthocyanin B ring in space. Deeper insights into the relationship between the conformational features and optical properties were acquired through TDDFT calculations. Based on the results, the most reasonable conformation of MvCG was proposed: the coumaryl group folded over the anthocyanin B ring in an aslant-parallel arrangement, generating intramolecular copigmentation interactions.


Assuntos
Antocianinas , Glucosídeos , Antocianinas/química , Cor , Glucosídeos/química , Modelos Teóricos
19.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(4): 440-446, 2022 Apr 15.
Artigo em Chinês | MEDLINE | ID: mdl-35527422

RESUMO

OBJECTIVES: To study the correlation of the expression of Lipin1 in visceral adipose tissue and Lipin2 in liver tissue with hepatic fat content in rats with intrauterine growth retardation (IUGR). METHODS: Pregnant rats were given a low-protein (10% protein) diet during pregnancy to establish a model of IUGR in neonatal rats. The pregnant rats in the control group were given a normal-protein (21% protein) diet during pregnancy. The neonatal rats were weighed and liver tissue was collected on day 1 and at weeks 3, 8, and 12 after birth, and visceral adipose tissue was collected at weeks 3, 8, and 12 after birth. The 3.0T 1H-magnetic resonance spectroscopy was used to measure hepatic fat content at weeks 3, 8, and 12 after birth. Real-time PCR was used to measure mRNA expression levels of Lipin2 in liver tissue and Lipin1 in visceral adipose tissue. Western blot was used to measure protein levels of Lipin2 in liver tissue and Lipin1 in visceral adipose tissue. A Pearson correlation analysis was performed to investigate the correlation of mRNA and protein expression of Lipin with hepatic fat content. RESULTS: The IUGR group had significantly higher mRNA and protein expression levels of Lipin1 in visceral adipose tissue than the control group at weeks 3, 8, and 12 after birth (P<0.05). Compared with the control group, the IUGR group had significantly lower mRNA and protein expression levels of Lipin2 in liver tissue on day 1 after birth and significantly higher mRNA and protein expression levels of Lipin2 at weeks 1, 3, 8, and 12 after birth (P<0.05). At week 3 after birth, there was no significant difference in hepatic fat content between the IUGR and control groups (P>0.05), while at weeks 8 and 12 after birth, the IUGR group had a significantly higher hepatic fat content than the control group (P<0.05). The protein and mRNA expression levels of Lipin1 were positively correlated with hepatic fat content (r=0.628 and 0.521 respectively; P<0.05), and the protein and mRNA expression levels of Lipin2 were also positively correlated with hepatic fat content (r=0.601 and 0.524 respectively; P<0.05). CONCLUSIONS: Upregulation of the mRNA and protein expression levels of Lipin1 in visceral adipose tissue and Lipin2 in liver tissue can increase hepatic fat content in rats with IUGR and may be associated with obesity in adulthood.


Assuntos
Retardo do Crescimento Fetal , Fígado , Adulto , Animais , Feminino , Expressão Gênica , Humanos , Fígado/metabolismo , Compostos Orgânicos , Gravidez , RNA Mensageiro/metabolismo , Ratos
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