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1.
Artigo em Inglês | MEDLINE | ID: mdl-35668004

RESUMO

OBJECTIVE: This study aimed to compare the clinical characteristics of patients with medication-related osteonecrosis of the jaw (MRONJ) and osteoporosis vs malignancy. STUDY DESIGN: The study included patients hospitalized with MRONJ between July 2013 and April 2021. These patients were assigned to the osteoporosis or malignancy groups according to their primary disease. Characteristics and clinical variables were recorded and compared. RESULTS: Nighty-one patients (107 MRONJ lesions) were included, with 12 (14 lesions) in the osteoporosis group and 79 (93 lesions) in the malignancy group. The osteoporosis and malignancy groups differed in their respective incubation periods (57.0 ± 42.8 vs 29.3 ± 19.8 months, respectively; P = .048), bisphosphonates cumulative dose (16,487.4 ± 14,268.8 mg alendronate vs 104.0 ± 79.9 mg zoledronic; P = .014), and rate of patients receiving antiangiogenic agents (0/12, 0.0% vs 48/79, 60.8%; P = .001). The groups were similar in their treatment outcomes, measured as successful surgeries (11/12, 91.7% vs 59/79, 74.7%; P = .351). CONCLUSIONS: For stage 2 or 3 MRONJ, patients with osteoporosis (exposed to oral bisphosphonates) developed MRONJ over a longer incubation period than patients with malignancy. The groups had similar responses to surgery.

2.
Food Microbiol ; 106: 104032, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35690438

RESUMO

Malt-induced premature yeast flocculation (PYF) is a sporadic problem within the brewing industry. The use of PYF malts is concomitant with a number of negative impacts on beer quality, including incomplete fermentation and/or flavor defects. Although malt-induced PYF is widely acknowledged, actions taken so far have proved insufficient to solve the PYF-related issues. To limit the detrimental effects of PYF malts on beer production, an adaptive laboratory evolution (ALE) process was applied in this study to an industrial lager brewing yeast strain (TT02), in an attempt to generate variant strains with improved fermentation performance in PYF wort. Through a batch fermentation-based adaptation process, evolved variants were isolated and screened for their phenotypic and metabolic traits. The investigation focused mainly on the tendency to remain in suspension, fermentation capacity and final acetaldehyde concentration. We successfully obtained a variant (TT02-30 T) with improved fermentation properties. The improvement was seen in worts prepared from different types of PYF malt as well as normal malt. Furthermore, ALE of lager brewing yeast in PYF wort yielded a wide array of mutations. Several changes in the genomes (copy number variation in flocculin encoding gene FLO1 and a missense SNP in a putative mitochondrial membrane protein coding gene FMP10) of the variant strains relative to the original strain were observed. These could potentially contribute to the improved yeast suspension during fermentation. Importantly, mutational enrichment in genes related to ion binding in PYF-evolved strains suggests the involvement of the yeast ion transportation process in dealing with the PYF stress. Our study demonstrates the possibility of attenuating yeast sensitivity to PYF malts over time through adaptive laboratory evolution via spontaneous mutation.


Assuntos
Cerveja , Saccharomyces cerevisiae , Cerveja/análise , Variações do Número de Cópias de DNA , Fermentação , Floculação , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo
4.
Psychiatry Res ; 314: 114658, 2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35660966

RESUMO

Many studies have identified changes in gene expression in brains of schizophrenia patients and their altered molecular processes, but the findings in different datasets were inconsistent and diverse. Here we performed the most comprehensive analysis of gene expression patterns to explore the underlying mechanisms and the potential biomarkers for early diagnosis in schizophrenia. We focused on 10 gene expression datasets in post-mortem human brain samples of schizophrenia downloaded from gene expression omnibus (GEO) database using the integrated bioinformatics analyses including robust rank aggregation (RRA) algorithm, Weighted gene co-expression network analysis (WGCNA) and CIBERSORT. Machine learning algorithm was used to construct the risk prediction model for early diagnosis of schizophrenia. We identified 15 key genes (SLC1A3, AQP4, GJA1, ALDH1L1, SOX9, SLC4A4, EGR1, NOTCH2, PVALB, ID4, ABCG2, METTL7A, ARC, F3 and EMX2) in schizophrenia by performing multiple bioinformatics analysis algorithms. Moreover, the interesting part of the study is that there is a correlation between the expression of hub genes and the immune infiltrating cells estimated by CIBERSORT. Besides, the risk prediction model was constructed by using both these genes and the immune cells with a high accuracy of 0.83 in the training set, and achieved a high AUC of 0.77 for the test set. Our study identified several potential biomarkers for diagnosis of SCZ based on multiple bioinformatics algorithms, and the constructed risk prediction model using these biomarkers achieved high accuracy. The results provide evidence for an improved understanding of the molecular mechanism of schizophrenia.

5.
BMC Pregnancy Childbirth ; 22(1): 501, 2022 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-35725418

RESUMO

BACKGROUND: Reproductive tract infections can cause serious adverse outcomes for pregnant women such as spontaneous abortion and preterm birth. However, it is unclear whether maternal reproductive tract infection before pregnancy would also be related to any adverse pregnancy outcomes. This study aims to investigate the association of maternal preconception reproductive tract infections with subsequent adverse pregnancy outcomes. METHODS: A retrospective cohort study was conducted in the Chongqing Municipality of China between April 2010 and December 2016. A total of 57,586 women (57,708 pregnancies) from all 39 counties of Chongqing who participated in the National Free Preconception Health Examination Project were included. They all took preconception examinations for gonorrhea, chlamydia, trichomoniasis, syphilis, bacterial vaginosis and candidiasis before pregnancy within one year. Primary outcomes included spontaneous abortion (< 28 weeks gestation), preterm birth (< 37 weeks gestation), macrosomia and low birthweight. RESULTS: Of the 57,708 pregnancies, 2438 (4.22%) had at least one type of reproductive tract infections. Compared with women who were not infected with any reproductive tract infection before pregnancy, women with reproductive tract infections had a higher rate of spontaneous abortion (7.88% vs. 5.62%, p < 0.001). After analyzing by each infection, there were few significant associations between pre-pregnancy infections and adverse outcomes. Preconception syphilis infection was significantly associated with increased odds of spontaneous abortion (aOR = 2.07, 95%CI 1.50-2.85), induced abortion/labour due to medical reasons (aOR = 1.60, 95%CI 1.01-2.54) and preterm birth (aOR = 1.60, 95%CI 1.12-2.30) after adjusting for potential confounders. Preconception trichomoniasis was intended to relate to a higher risk of spontaneous abortion (aOR = 1.65, 95%CI 1.01-2.71), but its impact seemed to be attributed to its co-infection with other RTIs. Women who were chlamydia or bacterial vaginosis positive before pregnancy showed higher odds of macrosomia (aOR = 2.00, 95% CI 1.07-3.74 for chlamydia; aOR = 1.58, 95% CI 1.06-2.34 for bacterial vaginosis). Preconception bacterial vaginosis might also be associated with higher risks of very preterm birth (aOR = 2.16, 95%CI 1.23-3.78) and large for gestational age (aOR = 1.36, 95%CI 1.02-1.81). CONCLUSIONS: Women with infections of the genital tract before pregnancy might also have increased risks of subsequent adverse outcomes including spontaneous abortion, preterm birth and macrosomia.


Assuntos
Aborto Espontâneo , Nascimento Prematuro , Infecções do Sistema Genital , Sífilis , Tricomoníase , Vaginose Bacteriana , Aborto Espontâneo/epidemiologia , Estudos de Coortes , Feminino , Macrossomia Fetal , Humanos , Recém-Nascido , Gravidez , Resultado da Gravidez/epidemiologia , Nascimento Prematuro/epidemiologia , Estudos Retrospectivos , Vaginose Bacteriana/complicações , Vaginose Bacteriana/epidemiologia
7.
Nature ; 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35705806

RESUMO

Exercise confers protection against obesity, type 2 diabetes and other cardiometabolic diseases1-5. However, the molecular and cellular mechanisms that mediate the metabolic benefits of physical activity remain unclear6. Here we show that exercise stimulates the production of N-lactoyl-phenylalanine (Lac-Phe), a blood-borne signalling metabolite that suppresses feeding and obesity. The biosynthesis of Lac-Phe from lactate and phenylalanine occurs in CNDP2+ cells, including macrophages, monocytes and other immune and epithelial cells localized to diverse organs. In diet-induced obese mice, pharmacological-mediated increases in Lac-Phe reduces food intake without affecting movement or energy expenditure. Chronic administration of Lac-Phe decreases adiposity and body weight and improves glucose homeostasis. Conversely, genetic ablation of Lac-Phe biosynthesis in mice increases food intake and obesity following exercise training. Last, large activity-inducible increases in circulating Lac-Phe are also observed in humans and racehorses, establishing this metabolite as a molecular effector associated with physical activity across multiple activity modalities and mammalian species. These data define a conserved exercise-inducible metabolite that controls food intake and influences systemic energy balance.

8.
Biomaterials ; 287: 121613, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35700621

RESUMO

Salmonella is a word-wide food-borne pathogen, which can cause severe enteritis and intestinal microbiota imbalance. Capsaicin (Cap), a food-based bioactive ingredient, has antibacterial and anti-inflammatory properties. However, its low solubility, low bioavailability and the irritation to digestive tract greatly limit its applications. Here, an intestinal responsively "nanotubes-in-microgel" composite carrier was constructed by capturing α-lactalbumin (α-lac) nanotubes in low-methoxy pectin microgels (LMP-NT) (52 µm). Cap was loaded in such system via hydrophobic interaction with a loading capacity of 38.02 mg/g. The LMP microgels remained stable and protected NT/Cap from early releasing in the gastric condition. It showed an excellent mucoadhesive capacity, which can prolong the intestinal retention up to 12 h and control release NT/Cap in intestine. Afterward, NT/Cap could penetrate across the mucus layer deeply and enter the intestinal villi epithelial cells efficiently. LMP-NT microgels achieved a mucoadhesive-to-penetrating transition in response to intestinal pH, improving the epithelium absorption and the in vivo bioavailability of Cap. Oral administration of LMP-NT/Cap could effectively alleviate enteritis caused by Salmonella infection and maintain the homeostasis of gut microbiota. Overall, this work suggested that LMP-NT composite microgels were promising for intestine-targeted and oral delivery of hydrophobic bioactive food compounds.

9.
Front Microbiol ; 13: 902110, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35707174

RESUMO

The viable but non-culturable (VBNC) state has been studied in detail in bacteria. However, it has received much less attention in eukaryotic cells. The induction of a VBNC beer-spoilage yeast (Brettanomyces bruxellensis) by hop bitter acids with different concentrations and its recovery were studied in this work. B. bruxellensis cells were completely induced into the VBNC state by treatment of 250 mg/L hop bitter acids for 2 h. The addition of catalase at a concentration of 2,000 U/plate on YPD agars enabled these VBNC cells to recover their culturability within 2 days. Moreover, the transcriptome profiling revealed that 267 and 197 genes were significantly changed upon VBNC state entry and resuscitation, respectively. The differentially expressed genes involved in the peroxisome activities, ABC transporter, organic acid metabolism, and TCA cycle were mainly downregulated in the VBNC cells. In contrast, the amino acid and carbohydrate metabolism, cell division, and DNA replication were promoted. This study supplies a theoretical basis for microbial risk assessment in the brewing industry.

10.
Carbohydr Polym ; 291: 119572, 2022 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-35698337

RESUMO

Integrating functionalities such as adhesiveness, self-healing, and conductivity on a polysaccharide-based hydrogel is highly desirable for ever-expanding practical applications, but there is always a challenge. Herein, an elaborately designed nanocomposite hydrogel is fabricated by the addition of highly conductive Ti3C2Tx MXene nanosheets into chondroitin sulfate (CS)/N, N-dimethylamino ethyl acrylate (DMAEA-Q) hydrogel network. Owing to the introduction of sulfonated Ti3C2Tx MXene nanosheets, the as-prepared nanocomposite hydrogels exhibit excellent stretchability (> 5000% strain), rapid self-healing ability (< 60 s), and high adhesiveness (≈ 100 kPa). The proposed hydrogel demonstrates an outstanding electrical conductivity up to 5.33 S/m, allowing real-time monitoring of the bending and stretching movements and full recovery. Furthermore, the SMC hydrogels exhibit fast and stable photothermal conversion performance due to the inherent photothermal behavior. Notably, multifunctional SMC hydrogels present real-time and reversible humidity sensing upon H2O-induced swelling/contraction of nanochannels between the Ti3C2Tx MXene interlayers, enabling respiration monitoring applications.


Assuntos
Adesivos , Hidrogéis , Condutividade Elétrica , Polissacarídeos , Titânio
11.
Chin Med J (Engl) ; 135(8): 962-970, 2022 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-35730373

RESUMO

BACKGROUND: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by complex and various clinical manifestations. The study aimed to analyze clinical features and cerebral magnetic resonance imaging (MRI) changes of hyperintense white matter (WM) lesions in SLE patients. METHODS: This was a retrospective study based on a consecutive cohort of 1191 SLE patients; 273 patients for whom cerebral MRI data were available were enrolled to assess hyperintense WM lesions associated with SLE. Patients were assigned to two groups, i.e., with or without hyperintense WM lesions. The MRI assessment showed that the hyperintense WM lesions could be classified into three categories: type A, periventricular hyperintense WM lesions; type B, subcortical hyperintense WM lesions; and type C, multiple discrete hyperintense WM lesions. The clinical and MRI characteristics were analyzed. Factors related to hyperintense WM lesions were identified by multivariate logistic regression analysis. RESULTS: Among the 273 SLE patients with available cerebral MRI scans, 35.9% (98/273) had hyperintense WM lesions associated with SLE. The proportions of types A, B, and C were 54.1% (53/98), 11.2% (11/98), and 92.9% (91/98), respectively. Fifty-one percents of the patients showed an overlap of two or three types. Type C was the most common subgroup to be combined with other types. Compared with those without hyperintense WM lesions, the patients with hyperintense WM lesions were associated with neuropsychiatric SLE (NPSLE), lupus nephritis (LN), hypertension, and hyperuricemia (P = 0.002, P = 0.018, P = 0.045, and P = 0.036, respectively). Significantly higher rates of polyserous effusions and cardiac involvement were found in the patients with hyperintense WM lesions (P = 0.029 and P = 0.027, respectively), and these patients were more likely to present with disease damage (P < 0.001). In addition, the patients with hyperintense WM lesions exhibited a higher frequency of proteinuria (P = 0.009) and higher levels of CD8+ T cells (P = 0.005). In the multivariate logistic analysis, hyperuricemia and higher CD8+ T cells percentages were significantly correlated with hyperintense WM lesions in SLE patients (P = 0.019; OR 2.129, 95% confidence interval [CI] 1.313-4.006 and P < 0.001; OR 1.056, 95% CI 1.023-1.098, respectively). CONCLUSIONS: Hyperintense WM lesions are common in SLE patients and significantly associated with systemic involvement, including NPSLE, LN, polyserous effusions, cardiac involvement, and disease damage. Hyperuricemia and a higher number of CD8+ T cells were independent factors associated with hyperintense WM lesions in SLE.

12.
Front Pharmacol ; 13: 908783, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35712711

RESUMO

Objective: Regulatory T cells (Tregs) are critical immune modulators to maintain immune homeostasis and limit pulmonary hypertension (PH). This study was aimed to identify Treg-related genes (TRGs) in PH. Methods: The gene expression profile from lungs of PH patients was retrieved from the Gene Expression Omnibus (GEO) database. The abundance of Tregs was estimated by the xCell algorithm, the correlation of which with differentially expressed genes (DEGs) was performed. DEGs with a |Pearson correlation coefficient| >0.4 were identified as TRGs. Functional annotation and the protein-protein interaction (PPI) network were analyzed. A gene signature for 25 hub TRGs (TRGscore) was generated by a single sample scoring method to determine its accuracy to distinguish PH from control subjects. TRGs were validated in datasets of transcriptional profiling of PH cohorts and in lung tissues of experimental PH mice. Results: A total of 819 DEGs were identified in lungs of 58 PAH patients compared to that of 25 control subjects of dataset GSE117261. In total, 165 of all these DEGs were correlated with the abundance of Tregs and identified as TRGs, with 90 upregulated genes and 75 downregulated genes compared to that of control subjects. The upregulated TRGs were enriched in negative regulation of multiple pathways, such as cAMP-mediated signaling and I-kappaB kinase/NF-kappaB signaling, and regulated by multiple genes encoding transcriptional factors including HIF1A. Furthermore, 25 hub genes categorized into three clusters out of 165 TRGs were derived, and we identified 27 potential drugs targeting 10 hub TRGs. The TRGscore based on 25 hub TRGs was higher in PH patients and could distinguish PH from control subjects (all AUC >0.7). Among them, 10 genes including NCF2, MNDA/Ifi211, HCK, FGR, CSF3R, AQP9, S100A8, G6PD/G6pdx, PGD, and TXNRD1 were significantly reduced in lungs of severe PH patients of dataset GSE24988 as well as in lungs of hypoxic PH mice compared to corresponding controls. Conclusion: Our finding will shed some light on the Treg-associated therapeutic targets in the progression of PH and emphasize on TRGscore as a novel indicator for PH.

13.
JACS Au ; 2(5): 1096-1104, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-35647601

RESUMO

Featuring high olefin selectivity, hexagonal boron nitride (h-BN) has emerged recently as an attractive catalyst for oxidative dehydrogenation of propane (ODHP). Herein, we report that dispersion of vanadium oxide onto BN facilitates the oxyfunctionalization of BN to generate more BO x active sites to catalyze ODHP via the Eley-Rideal mechanism and concurrently produce nitric oxide to initiate additional gas-phase radical chemistry and to introduce redox VO x sites to catalyze ODHP via the Mars-van Krevelen mechanism, all of which promote the catalytic performance of BN for ODHP. As a result, loading 0.5 wt % V onto BN has doubled the yield of light alkene (C2-C3) at 540-580 °C, and adding an appropriate concentration of NO in the reactants further enhances the catalytic performance. These results provide a potential strategy for developing efficient h-BN-based catalysts through coupling gas-phase and surface reactions for the ODHP process.

15.
Nat Nanotechnol ; 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-35606442

RESUMO

Friction and wear are detrimental to functionality and reduce the service life of products with mechanical elements. Here, we unveil the atomic-scale friction of a single tungsten asperity in real time through a high-resolution transmission electron microscopy investigation of a nanocontact in countermotion, induced through a piezo actuator. Molecular dynamics simulations provide insights into the sliding pathway of interface atoms and the dynamic strain/stress evolution at the interface. We observe a discrete stick-slip behaviour and an asynchronous process for the accumulation and dissipation of the strain energy together with the non-uniform motion of interface atoms. Our methodology allows for studying in situ atomic-friction phenomena and provides insights into friction phenomena at the atomic scale.

16.
Biomed Pharmacother ; 151: 113173, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35623165

RESUMO

Exposure to crystalline silica (CS) results in a persistent pulmonary inflammatory response, which results in abnormal tissue repair and excessive matrix deposition. Due to vague pathogenesis, there is virtually no practical therapeutic approach. Here we showed the pharmacological effects of TUDCA on CS-induced pulmonary inflammation and fibrosis. It also helped a faster recovery of CS-impaired pulmonary function. Mechanistically, TUDCA suppressed interferon (IFN)-γ and interleukin (IL)-17A productions by pulmonary helper T (Th) cells. We demonstrated that CS-boosted cytokine-producing Th cells were effector memory (TEM) phenotype. TUDCA decreased the pathogenic TEM cells expansion in the lung. Using in vivo labeling method, we discovered the TEM cells were lung tissue residency with CD103 expression. TUDCA's anti-fibrotic effects were linked to decreasing IFN-γ producing CD103- TEM-like and IL-17A producing CD103+ TRM-like T cells as well as restricting TRM-like Treg cells in the lung. Specifically, TUDCA could restrain CD103+ TRM-like Treg cell proliferation but not limit the CD103- ones. Further characterization study proved that though the Tregs originally came from the thymus, the expressing levels of ST-2 were different, which provides insights into TUDCA's various effects on cell proliferation. Collectively, our data paved the way to understanding the pathogenesis of silicosis and may provide new treatments for this pulmonary fibrotic disease.


Assuntos
Silicose , Linfócitos T CD8-Positivos/metabolismo , Fibrose , Humanos , Memória Imunológica , Pulmão/patologia , Dióxido de Silício , Silicose/metabolismo , Ácido Tauroquenodesoxicólico
17.
Sci Total Environ ; 838(Pt 2): 156141, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35609696

RESUMO

Persistently high concentrations of antibiotics have been reported in soils worldwide due to the intensive use of veterinary antibiotics, and continuous adsorption and transport of various antibiotics in soils occur, posing a significant threat to the environment and human health. This study systematically reviews the spatial distribution and ecological risk of four commonly detected antibiotic residues in soil in China, including sulphonamides (SAs), fluoroquinolones (FQs), tetracyclines (TCs) and macrolides (MLs), using various models, such as redundancy analysis (RDA), principal coordinate analysis (PCoA) and structural equation modelling (SEM). Antibiotic residual concentration data were obtained from relevant repositories and the literature. The results suggest a high level of antibiotic pollution and ecological risk in the largest urban agglomerations (LUAs), including Beijing-Tianjin-Hebei (BTH), Yangtze River Delta (YRD) and Guangdong-Hong Kong-Macao Greater Bay Area (GBA), with a 100% detection rate. SAs, FQs, TCs and MLs were the dominant antibiotic residues in soils, mainly attributed to manure fertilization and wastewater reuse in agriculture. These antibiotic concentrations ranged from 10-3 to 103 µg kg-1, and their ecological risk varied significantly across different regions of China, with SAs posing the most serious ecological risk to the soil environment (p < 0.05). These models established a significant association (p < 0.05) between the physicochemical properties of antibiotics and land-use type (LUT) with antibiotic residues in soil. The structure of the antibiotic exerted the greatest influence on antibiotic residues, followed by the LUT, while regional differences had the weakest effect.


Assuntos
Poluentes do Solo , Solo , Antibacterianos/análise , China , Monitoramento Ambiental/métodos , Fluoroquinolonas/análise , Humanos , Macrolídeos/análise , Solo/química , Poluentes do Solo/análise , Tetraciclinas/análise
18.
Nat Neurosci ; 25(5): 646-658, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35501380

RESUMO

Midbrain dopamine (DA) and serotonin (5-HT) neurons regulate motivated behaviors, including feeding, but less is known about how these circuits may interact. In this study, we found that DA neurons in the mouse ventral tegmental area bidirectionally regulate the activity of 5-HT neurons in the dorsal raphe nucleus (DRN), with weaker stimulation causing DRD2-dependent inhibition and overeating, while stronger stimulation causing DRD1-dependent activation and anorexia. Furthermore, in the activity-based anorexia (ABA) paradigm, which is a mouse model mimicking some clinical features of human anorexia nervosa (AN), we observed a DRD2 to DRD1 shift of DA neurotransmission on 5-HTDRN neurons, which causes constant activation of these neurons and contributes to AN-like behaviors. Finally, we found that systemic administration of a DRD1 antagonist can prevent anorexia and weight loss in ABA. Our results revealed regulation of feeding behavior by stimulation strength-dependent interactions between DA and 5-HT neurons, which may contribute to the pathophysiology of AN.


Assuntos
Dopamina , Serotonina , Animais , Anorexia , Neurônios Dopaminérgicos , Mesencéfalo , Camundongos , Neurônios/fisiologia
19.
J Biomater Appl ; : 8853282221080525, 2022 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-35594029

RESUMO

Co-doping of multiple ions can effectively adjust the biological properties of hydroxyapatite (HA) for various biomedical applications. In this study, we prepared Sr2+ and Cu2+ double-doped hollow HA and characterized them by SEM, EDS, XRD, FTIR, and other methods. We found that Sr2+ and Cu2+ were uniformly distributed in the hollow carbonic acid HA microspheres. As the proportion of metal elements increases, the microspherical appearance and crystallinity properties also change. In addition, we also prepared porous titanium scaffolds through 3D printing technology and constructed composite scaffolds of porous titanium scaffolds, Sr2+ and Cu2+ double-doped HA, and gelatin. In vitro cell experiments and bacterial experiments, the composite scaffolds, especially the 10%Cu-10%Sr- HA/Gel/Ti group scaffolds, have good biocompatibility and integration with bone tissues, promoting the proliferation and differentiation of BMSCs while having excellent antibacterial properties. These composite scaffolds can simultaneously achieve bone defect filling, osteoblast differentiation, and antibacterial functions, owning broad clinical application prospects.

20.
Ther Adv Psychopharmacol ; 12: 20451253221079971, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35510088

RESUMO

Numerous case reports of acute pancreatitis (AP) induced by olanzapine have been published. Little is, however, known about the clinical features of olanzapine-induced AP. The aim of the study was to explore the clinical characteristics of olanzapine-induced AP. We collected literature on AP cases induced by olanzapine from 1996 to April 2021 for retrospective analysis in Chinese and English. The median time to onset of olanzapine-induced acute pancreatic symptoms was 12 (range = 0.86-216) weeks in 25 patients. The clinical features of AP range from asymptomatic elevation of blood amylase/lipase levels to digestive system symptoms (abdominal pain, vomiting, and nausea) and even death in a small number of patients. Laboratory tests showed varying degrees of elevated serum amylase and lipase levels, along with high blood sugar and high triglyceride levels in some patients. Computed tomography showed acute edematous pancreatitis, acute hemorrhagic pancreatitis, and acute necrotizing pancreatitis in the patients. The patients' symptoms were completely relieved and high triglyceride levels gradually returned to normal levels after olanzapine was stopped. Some patients with hyperglycemia still needed hypoglycemic therapy. AP is a rare adverse effect of olanzapine. Clinicians should be aware of such complications and monitor pancreatin.

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