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1.
Colloids Surf B Biointerfaces ; 208: 112126, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34600360

RESUMO

Selective cancer cell targeting, controlled drug release, easy construction and multiple therapeutic modalities are some of the desirable characteristics of drug delivery systems. We designed and built simple capsule-like molecular imprinted polymer (MIP)-based nanoparticles for targeted and chemo-photothermal synergistic cancer therapy. Using dopamine (DA) as functional monomer, cross-linking agent as well as photo-thermal agent, ZIF-8 (zeoliticimidazolate framework-8) as drug carrier, epitope of EGFR (epidermal growth factor receptor) as template molecules, molecular imprinted polymer (MIP) drug carrier was constructed. The ability of MIP layer to bind to EGFR epitope endowed the MD (DOX@MIP) particles to recognize EGFR-overexpressing cancer cells, while the pH-responsiveness and photothermal conversion ability of PDA (polydopamine) achieved chemo-photothermal synergistic effects upon NIR irradiation. Taken together, the MD nanoparticles integrated cancer cell targeting recognition, intelligent drug release, biocompatibility and chemo-photothermal effects, and is therefore a promising tool for targeted cancer therapy with minimal toxicity to normal cells, as well as tumor imaging.

2.
Artigo em Inglês | MEDLINE | ID: mdl-34629248

RESUMO

BACKGROUND AND AIMS: Patients with multiple metabolic diseases are at high risk for the occurrence and death of COVID-19. Little is known about patients with underweight and metabolically healthy obesity. The aim of this study is to evaluate the impact of BMI and COVID-19 mortality in hospitalized patients, and also explore the association in different metabolically healthy (MHS) and unhealthy status (MUS). METHODS AND RESULTS: A retrospective cohort study based on 3019 inpatients from Wuhan was conducted. Included patients were classified into four groups according the BMI level (underweight, normal weight, overweight and obesity), and patients with at least one of the metabolic abnormalities (diabetes, hypertension, dyslipidemia) was defined as MUS. Multiple Cox model was used to calculate the hazard ratio (HR). Compared to patients with normal weight, the HRs of overweight and obesity for COVID-19 mortality were 1.91 (95%CI:1.02-3.58) and 2.54 (95%CI:1.22-5.25) respectively in total patients, and 2.58 (95%CI:1.16-5.75) and 3.89 (95%CI:1.62-9.32) respectively in the elderly. The HR of underweight for COVID-19 mortality was 4.58 (95%CI:1.56-13.48) in the elderly. For different metabolic statuses, both underweight, overweight and obesity had obviously negative association with COVID-19 mortality in total and elderly patients with MUS. However, no significance was found in non-elderly and patients with MHS. CONCLUSION: Not only overweight or obesity, but also underweight can be associated with COVID-9 mortality, especially in the elderly and in patients with MUS. More large-scale studies are needed for patients with underweight and metabolically healthy overweight or obesity.

3.
Front Med (Lausanne) ; 8: 729928, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34631749

RESUMO

Background: To explore the association and understand gender disparities between nutritional status and quality of life among centenarians. Methods: It was a full-sample survey of centenarians conducted in Hainan that included a total of 1,002 eligible centenarians whose age had been verified. The Mini Nutritional Assessment - Short Form (MNA-SF) questionnaire and the EuroQol five dimensions visual analog scale (EQ-5D-VAS) were used to measure participants' nutritional status and quality of life, respectively. Findings: In the 1002 centenarians (822 women and 180 men), 797 (79.5%) (79.5%) reported multimorbidity. The adjusted standardized ß estimate association between the MNA-SF and EQ-5D scores was 0.508 in the complete sample. With reference to the normal nutrition group, the standardized ß estimate of the association between EQ-5D score and nutritional status were -0.179 and -0.583 for the at risk of malnutrition and malnutrition groups, respectively (both P <0.001). Nutritional status significantly affected the five dimensions of quality of life, particularly mobility and self-care. Compared with the normal nutrition group, the malnutrition group had greater odds of low mobility [Odds ratio (OR)=23.15; 95% CI: 9.81-54.64] and low self-care (OR=24.58; 95% CI: 12.62-47.89). Among males, nutritional status was significantly associated with the usual activities and anxiety/depression dimensions after adjustment. Female participants had results similar to the general population. Interpretation: Malnutrition and being at risk of malnutrition is prevalent among centenarians. Maintaining normal nutritional status is an important protective factor and should receive more attention to improve centenarians' quality of life.

4.
Plant Biotechnol J ; 2021 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-34669232

RESUMO

Cytosine base editors (CBEs) can install a predefined stop codon at the target site, representing a more predictable and neater method for creating genetic knockouts without altering the genome size. Due to the enhanced predictability of the editing outcomes, it is also more efficient to obtain homozygous mutants in the first generation. With the recent advancement of CBEs on improved editing activity, purify and specificity in plants and animals, base editing has become a more appealing technology for generating knockouts. However, there is a lack of design tools that can aid the adoption of CBEs for achieving such a purpose, especially in plants. Here, we developed a user-friendly design tool named CRISPR-BETS (base editing to stop), which helps with guide RNA (gRNA) design for introducing stop codons in the protein-coding genes of interest. We demonstrated in rice and tomato that CRISPR-BETS is easy-to-use, and its generated gRNAs are highly specific and efficient for generating stop codons and obtaining homozygous knockout lines. While we tailored the tool for the plant research community, CRISPR-BETS can also serve non-plant species.

5.
Cell Death Dis ; 12(9): 843, 2021 09 11.
Artigo em Inglês | MEDLINE | ID: mdl-34511597

RESUMO

Renal fibrosis is a common pathological process that occurs with diverse etiologies in chronic kidney disease. However, its regulatory mechanisms have not yet been fully elucidated. Ferroptosis is a form of non-apoptotic regulated cell death driven by iron-dependent lipid peroxidation. It is currently unknown whether ferroptosis is initiated during unilateral ureteral obstruction (UUO)-induced renal fibrosis and its role has not been determined. In this study, we demonstrated that ureteral obstruction induced ferroptosis in renal tubular epithelial cells (TECs) in vivo. The ferroptosis inhibitor liproxstatin-1 (Lip-1) reduced iron deposition, cell death, lipid peroxidation, and inhibited the downregulation of GPX4 expression induced by UUO, ultimately inhibiting ferroptosis in TECs. We found that Lip-1 significantly attenuated UUO-induced morphological and pathological changes and collagen deposition of renal fibrosis in mice. In addition, Lip-1 attenuated the expression of profibrotic factors in the UUO model. In vitro, we used RSL3 treatment and knocked down of GPX4 level by RNAi in HK2 cells to induce ferroptosis. Our results indicated HK2 cells secreted various profibrotic factors during ferroptosis. Lip-1 was able to inhibit ferroptosis and thereby inhibit the secretion of the profibrotic factors during the process. Incubation of kidney fibroblasts with culture medium from RSL3-induced HK2 cells promoted fibroblast proliferation and activation, whereas Lip-1 impeded the profibrotic effects. Our study found that Lip-1 may relieve renal fibrosis by inhibiting ferroptosis in TECs. Mechanistically, Lip-1 could reduce the activation of surrounding fibroblasts by inhibiting the paracrine of profibrotic factors in HK2 cells. Lip-1 may potentially be used as a therapeutic approach for the treatment of UUO-induced renal fibrosis.

6.
Gastroenterol Rep (Oxf) ; 9(4): 329-338, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34567565

RESUMO

Background: Infliximab (IFX) is effective at inducing and maintaining clinical remission and mucosal healing in patients with Crohn's disease (CD); however, 9%-40% of patients do not respond to primary IFX treatment. This study aimed to construct and validate nomograms to predict IFX response in CD patients. Methods: A total of 343 patients diagnosed with CD who had received IFX induction from four tertiary centers between September 2008 and September 2019 were enrolled in this study and randomly classified into a training cohort (n = 240) and a validation cohort (n = 103). The primary outcome was primary non-response (PNR) and the secondary outcome was mucosal healing (MH). Nomograms were constructed from the training cohort using multivariate logistic regression. Performance of nomograms was evaluated by area under the receiver-operating characteristic curve (AUC) and calibration curve. The clinical usefulness of nomograms was evaluated by decision-curve analysis. Results: The nomogram for PNR was developed based on four independent predictors: age, C-reactive protein (CRP) at week 2, body mass index, and non-stricturing, non-penetrating behavior (B1). AUC was 0.77 in the training cohort and 0.76 in the validation cohort. The nomogram for MH included four independent factors: baseline Crohn's Disease Endoscopic Index of Severity, CRP at week 2, B1, and disease duration. AUC was 0.79 and 0.72 in the training and validation cohorts, respectively. The two nomograms showed good calibration in both cohorts and were superior to single factors and an existing matrix model. The decision curve indicated the clinical usefulness of the PNR nomogram. Conclusions: We established and validated nomograms for the prediction of PNR to IFX and MH in CD patients. This graphical tool is easy to use and will assist physicians in therapeutic decision-making.

7.
BMC Genomics ; 22(1): 668, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34525971

RESUMO

OBJECTIVE: From genome-wide association studies, brain-derived neurotrophic factor (BDNF) locus on chromosome 11 was the only SNP associated with both smoking and body mass index (BMI) in European, African and Asian population. This study aims to explore the unique genetic predisposition to obesity in former smokers by examining the effects of BDNF on BMI and waist circumference (WC). METHODS: The study design is case-control study with a cohort validation in supplementary. We included 15,072 ethnic Chinese participants in the Guangzhou Biobank Cohort Study (GBCS) with data of four BDNF SNPs related to both BMI and smoking behavior. We used baseline smoke exposure data in 2003-2007 and follow-up outcomes of general obesity (by BMI) and central obesity (WC) in 2008-2012. Odds ratios (ORs) and 95% confidence intervals (CIs) for general obesity and central obesity associated with these SNPs were derived from logistic regression. RESULTS: Of 15,072 participants (3169 men and 11,903 women), 1664 (11.0%) had general and 7868 (52.2%) had central obesity. In 1233 former smokers, the rs6265 GG, versus AA, genotype was associated with higher risks of general obesity (OR = 1.79, 95% CI = 1.06-3.01) and central obesity (OR = 2.08, 95% CI = 1.47-2.92) after adjustment. These associations were not significant in never or current smokers. In former heavy (≥20 cigarettes/day) smokers, the rs6265 GG genotype showed a higher odds for general obesity (OR = 2.15, 95% CI = 1.05-4.40), while no association was found in former light (1-9 cigarettes/day) smokers. Similar results were found for the association of rs6265 with central obesity and for the associations of other two BDNF SNPs (rs4923457 and rs11030104) with both general and central obesity. CONCLUSIONS: We firstly identified the genetic predisposition (BDNF SNPs) to general and central obesity in former smokers, particularly in former heavy smokers. The different associations of the SNPs for general/central obesity in different smoke exposure groups may be related to the competitive performance of the sites and epigenetic modification, which needs further study.


Assuntos
Fator Neurotrófico Derivado do Encéfalo , Estudo de Associação Genômica Ampla , Índice de Massa Corporal , Fator Neurotrófico Derivado do Encéfalo/genética , Estudos de Casos e Controles , Estudos de Coortes , Feminino , Humanos , Masculino , Obesidade/genética , Fumantes
8.
Transl Androl Urol ; 10(8): 3432-3439, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532268

RESUMO

Background: To perform a prospective, randomized, single center study to investigate the efficacy of combined use of curcumin, an anti-inflammatory agent, with the best standard management (BSM, tamsulosin and finasteride) in benign prostatic hyperplasia (BPH) patients. Methods: One hundred and twenty-two consecutive patients were randomized to receive tamsulosin 0.2 mg, finasteride 5 mg, and curcumin 2,250 mg once a day (curcumin + BSM group, n=61) versus tamsulosin 0.2 mg, finasteride 5 mg, and placebo (BSM group, n=61) for 6 months. The safety of treatments and their efficacy on improving waist circumference (WC), periprostatic fat thickness (PPFT), lower urinary tract symptoms (LUTS), and sexual function were assessed at baseline and month 6. Results: One hundred and sixteen patients completed the whole procedure (116/122, 95.1%). There were significant improvements in prostate volume (PV), maximum flow rate (Qmax), the International Prostate Symptom Score (IPSS), IPSS-voiding subscore (IPSS-V), IPSS-storage subscore (IPSS-S), and quality of life (QoL) from baseline after treatment in both groups. Additionally, both WC and PPFT decreased significantly after treatments than those at baseline in the curcumin + BSM group. Also, WC and PPFT in the curcumin + BSM group were significantly lower than those in the BSM group. In addition, IPSS-S, QoL score, and the 5-item version of the International Index of Erectile Function (IIEF-5) in the curcumin + BSM group improved significantly compared with those in the BSM group. Conclusions: We conclude that curcumin combined with tamsulosin and finasteride has more beneficial effects in reducing PPFT, protecting erectile function, improving urinary retention symptoms, and QoL scores in BPH patients compared to tamsulosin and finasteride alone. Trial Registration: Chinese Clinical Trial Registry ChiCTR2100043800.

9.
Transl Androl Urol ; 10(8): 3423-3431, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532267

RESUMO

Background: Benign prostatic hyperplasia (BPH) is a common micturition disorder in middle-aged and elderly males, and it is one of the most common urology-related diseases worldwide. However, standard therapeutic drugs (α1-receptor blockers + 5α reductase inhibitors) do not provide anti-inflammatory or anti-infective effects. The Phellodendron Bawei tablet is a proprietary Chinese medicine with anti-inflammatory and anti-infective effects. Here, we analyzed whether the combination of standard therapeutic drugs and Phellodendron Bawei tablets has more advantages than placebo with standard management in improving the lower urinary tract symptoms (LUTs), sleep quality, sexual function, and medication compliance in patients with BPH. Methods: This study was a prospective, double-blind, single-center, 6-month clinical trial in patients with BPH. Male patients, 45-75 years old, a history of moderate-to-severe BPH/LUTs for more than 6 months, moderate-to-severe LUTs [International Prostate Symptom Score (IPSS) ≥8], maximum urine flow rate (Qmax) of <15 mL/s, and prostate volume (PV) of >30 mL. All patients were randomly divided into two cohorts at baseline. The standard management (SM) group was treated with tamsulosin + finasteride + placebo, while the experimental group was treated with tamsulosin + finasteride + Phellodendron Bawei tablets. The clinical indicators were as follows: Age, body mass index (BMI), blood prostate-specific antigen (PSA), PV, Qmax, IPSS; IPSS voiding subscore (IPSS-V), IPSS storage subscore (IPSS-S), and IPSS quality of life (IPSS-QOL)], five-item version of the International Index of Erectile Function (IEFF-5) score, Athens Insomnia Scale (AIS) score, and the Medication Adherence Questionnaire (MAQ). And adverse drug reactions were observed. Student's t-test was used to analyze results. Results: We randomly divided 120 patients into two groups, with 60 patients in each group, and a total of 105 patients completed the study. IPSS-S (P=0.027) and AIS scores (P<0.001) improved more significantly in the Phellodendron Bawei tablets + SM group, and the MAQ score in this group was lower (P=0.003). Conclusions: Phellodendron Bawei tablets combined with α1-receptor blockers and 5α-reductase inhibitors can improve lower urinary tract symptoms associated with urine storage, sleep quality, and medication compliance in patients with benign prostatic hyperplasia compared to placebo with standard management. Trial Registration: Chinese Clinical Trial Registry ChiCTR2100046463.

10.
Ann Transl Med ; 9(15): 1246, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532383

RESUMO

Background: Benign prostatic hyperplasia (BPH) is a common urological condition in aging men. While dihydroartemisinin (DHA) exhibits a wide range of pharmacological activities, to date, there have been no studies examining the effects of DHA on BPH. Methods: An in vivo BPH model was constructed in rats via daily subcutaneous injection of testosterone propionate (TP) for 28 consecutive days. Rats were randomly distributed into four groups and treated as follows: (I) control; (II) TP treatment; (III) TP and finasteride treatment (positive control); and (IV) TP and DHA treatment. At the end of the experiment, rats were sacrificed and the prostate weight, prostate index, thickness of the epithelium, collagen deposition, serum dihydrotestosterone (DHT) levels, 5α-reductase 2 (5AR-2) expression, and proliferating cell nuclear antigen (PCNA) levels in the prostate were examined. Normal human prostatic epithelial RWPE-1 cells were used in in vitro experiments to further investigate the anti-proliferative effects of DHA. Results: TP increased the prostate weight and prostate index in rats, and this effect was reduced with DHA treatment. In addition, DHA attenuated the morphological changes and collagen deposition in the prostate tissue induced by TP. Furthermore, DHA reduced the expression of PCNA, serum DHT, and prostatic 5AR-2 in rats with TP-induced BPH. In vitro analysis revealed that DHA significantly inhibited the proliferation of TP-treated RWPE-1 cells. Conclusions: DHA significantly inhibited the development of BPH by suppressing serum DHT levels, prostatic 5AR-2 expression, and the proliferation of benign prostatic epithelial cells. Thus, DHA is a novel medicinal agent with potential therapeutic efficacy in the treatment of patients with BPH.

11.
Ann Transl Med ; 9(16): 1311, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34532448

RESUMO

Background: Acute pyelonephritis (APN), an acute and severe kidney infection, is usually treated with antibiotics. However, APN treatment has become increasingly challenging because of bacterial resistance. Adiponectin, an adipokine, has recently been reported to exhibit profound anti-inflammatory and anti-apoptotic effects. However, the effect of adiponectin on the outcomes of APN treatment remains unclear. In this study, we aimed to investigate the effects of adiponectin on APN and the mechanisms underlying these effects. Methods: Wild-type C57 mice and adiponectin-knockout (KO) mice were divided into 6 groups: the wild-type control group, the wild-type model group, the wild-type adiponectin intervention group, the KO control group, the KO model group, and the adiponectin-KO intervention group. We measured white blood cell (WBC) and neutrophil counts (NC) using a multispecies hematology analyzer; tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) using enzyme-linked immunosorbent assay (ELISA); blood urea nitrogen (BUN) and serum creatinine (SCr) levels using colorimetry; and the protein levels of JAK2, STAT3, p-JAK2, p-STAT3, Bcl-2, and Bax in renal tissues using western blot analysis. Apoptotic cells were detected using the transferase-mediated dUTP nick end labelling (TUNEL) assay. Results: Compared to the wild-type mice, the KO mice showed a more severe inflammatory response and kidney damage after Escherichia coli infection. After treatment with exogenous adiponectin injection, the inflammatory response, oxidative stress, and kidney damage were partly alleviated. Adiponectin KO led to JAK2/STAT3 signaling activation, and exogenous adiponectin administration inactivated JAK2/STAT3 signaling in the APN model. APN can lead to an increase in the level of the protein Bax and a decrease in the level of the bcl-2 protein, thereby increasing apoptosis; this effect was inhibited by adiponectin. Conclusions: Through use of a pyelonephritis mouse model, we demonstrated that adiponectin might alleviate renal cell apoptosis and inflammatory response by inactivating the JAK2/STAT3 signaling pathway.

12.
J Hazard Mater ; 416: 125721, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492775

RESUMO

Chlorophenols (CPs) are toxic contaminants that tend to accumulate in textile dyeing sludge and pose a threat to the environment through the disposal process. To comprehensively evaluate CPs in sludge, the characteristics and risks of CPs from five textile dyeing plants (TDPs) were investigated in this study. The total concentration of 19 CPs (Σ19 CPs) varied from 170.90 to 6290.30 ng g-1 dry weight (dw), among which high-chlorine phenols accounted for the greatest proportion. The ecological screening level (ESL) of CPs was used to judge their pollution levels, while the risk quotient (RQ) value and dioxin conversion rate were used to analyze their potential risk. The results indicated that CPs may pose a moderate to high risk to the environment. The Fenton process was used to condition the hazardous sludge, and a higher content of CPs was found after conditioning. A lower rate of CP increase was achieved with a reagent dose of 180 mmol/L, H2O2:Fe2+ = 1:1, pH of 3-4 and reaction time of 30 min. In summary, the work helps to address the general knowledge gap in the textile dyeing industry and provides a reference for further research.


Assuntos
Clorofenóis , Esgotos , Peróxido de Hidrogênio , Indústria Têxtil , Têxteis
13.
Artigo em Inglês | MEDLINE | ID: mdl-34467487

RESUMO

The high toxicity and low volatility of PCDD/Fs prevent detailed study of their catalytic degradation removal characteristics. In this study, 1,2-dichlorobenzene (1,2-DCBz) was initially used as a model to investigate the catalytic characteristics of various vanadium-based catalysts prepared by different methods. Then, the optimized catalyst was used for catalytic degradation of real PCDD/Fs at low temperatures based on a self-made stable source. The VOx/TiO2 catalysts synthesized by the mechanochemical method (VTi-MC2) had a higher 1,2-DCBz removal efficiency (>85%) and stability (> 420 min) at low temperatures (< 200 °C) compared to VTi-SG (sol-gol method) and VTi-WI (wetness impregnation method). The physicochemical properties of catalysts were studied using comprehensive characterization. It was found that the VTi-MC2 has better VOx species distribution and possesses the highest V5+ species and surface adsorbed oxygen content, which are the key factors that contributed to the higher removal efficiency. Accordingly, the mechanochemical method can be used to control the physicochemical properties of catalysts by adjusting the milling parameters. The optimum ball milling time is 2 h and a suitable precursor is NH4VO3 for VOx/TiO2. Moreover, the removal efficiency and catalytic degradation efficiency of PCDD/Fs in gas phase catalyzed by VTi-MC2 were 97% and 50% respectively, within a range of temperatures below 200 °C, which are both higher than those reported research. In general, the mechanochemical strategy employed in this study provides a means for seeking more efficient catalysts used for low-temperature degradation of various trace organic pollutants.

14.
Front Cardiovasc Med ; 8: 603877, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34490360

RESUMO

Objective: This study aimed to investigate the distribution of ideal cardiovascular health (ICH) indicators among the oldest-old and centenarians and explore their relationships with disability and health-related quality of life of this population. Methods: One thousand two centenarians from China Hainan Centenarian Cohort Study and 798 oldest-old from the China Hainan Oldest-old Cohort study were the target subjects in this analysis. ICH status, disability, and health-related quality of life of study subjects were assessed. Findings: The median value of ICH indicators among centenarians and the oldest-old is 4 (4-5) and 3 (3-5), respectively. The ICH indicators with the highest percentage of ideal level/status are fasting plasm glucose (FPG) (90.2% of study subjects are at the ideal level), BMI (89.8% of study subjects are at the ideal level), and smoking (89.4% of study subjects are at the ideal status). The disability rates of basic activities of daily living (BADL) and instrumental activities of daily living (IADL) decrease with the increasing number of ICH indicators. The EQ VAS and EQ-5D score show an increasing trend along with the increasing number of ICH indicators (p < 0.05). After adjusting related covariates, the risk of disability and lower health-related quality of life decreased gradually as the number of ICH metrics increased (p < 0.05). Interpretation: The ICH metrics of centenarians and oldest-old were at a relatively good level, and there was a strong and independent relationship between the number of ICH indicators and disability as well as the lower health-related quality of life.

15.
Ann Palliat Med ; 10(8): 8808-8817, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34488369

RESUMO

BACKGROUND: With the development of instrument technology, the functions and detection methods of automatic blood cell analyzers have become more complex. To ensure optimal clinical applicability, it is crucial to select an automatic blood cell analyzer with excellent clinical detection performance. This study evaluated the latest Mindray BC-6800Plus automatic blood cell analyzer and assessed its performance in the detection of nucleated red blood cells (NRBCs). METHODS: A total of 490 clinical blood samples were used to assess the performance of the instrument, including parameters such as precision, linearity, conformity rate of manual microscopic examination, carryover, and limit of quantitation. RESULTS: The instrument showed a small carryover (≤0.02) and excellent linearity (R2≥0.9986). The reproducibility of the sample tests was satisfactory, and the coefficient of variation (CV) of the test results [0.98-1.72% and 0.62-6.97% for white blood cells (WBCs) and NRBCs, respectively] were significantly lower than that declared by the manufacturer (2.5% and 20% for WBCs and NRBCs, respectively). Thus, the BC-6800Plus satisfies the requirements of clinical testing. Two separate Mindray BC-6800Plus machines were tested and found to be in good agreement with each other and with manual microscopy methods. Furthermore, WBC and NRBC counts were highly consistent with results obtained using the XN-9100 blood analyzer. CONCLUSIONS: The Mindray BC-6800Plus is an excellent analyzer that can provide timely and accurate reports for clinical laboratory detection of NRBC.


Assuntos
Hematologia , Contagem de Células Sanguíneas , Eritrócitos , Contagem de Leucócitos , Reprodutibilidade dos Testes
16.
Sci Total Environ ; 803: 150057, 2021 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-34500269

RESUMO

Black carbon (BC), characterized by high aromaticity and stability, has been recognized as a substantial fraction of the carbon pool in soil and sediment. The effect of BC on the particulate organic carbon (POC) pool in lake water, which is an important medium of carbon transmission and transformation, has not been thoroughly studied. The investigations of BC composition and distribution, POC, polycyclic aromatic hydrocarbons (PAHs), and stable carbon and nitrogen isotopes were conducted in a eutrophic urban lake, Taihu Lake, which is the third largest freshwater lake in China. The results indicate that the BC is composed of 55 ± 12% char and 45 ± 12% soot and accounted for 12 ± 6% of POC (the maximum value is 31%). The comparatively high levels of BC and char are distributed in the northern Taihu Lake, especially in Meiliang Bay (0.72 ± 0.38 mg L-1 and 0.45 ± 0.24 mg L-1). The distribution of soot presents a declining trend from the lakeshore to the central lake, particularly in the northern, western, and southern lakes. Source apportionment results from positive matrix factorization of PAHs suggest that consumption of fossil fuel (79 ± 20%) is the dominant source of BC, which agrees with the low ratio of char/soot (1.41 ± 0.71) and relatively depleted δ13C. The covariation of BC and PAHs and terrestrial dissolved organic carbon indicate that the effect of terrestrial input significantly regulates the distribution of BC in Taihu Lake, which is reflected in the high BC value along the lakeshore.

17.
Front Neurosci ; 15: 663015, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34366771

RESUMO

Previous studies have provided evidence about the brain plasticity effects of musical training, however, the issue of how expertise in music styles induced by Chinese or Western musical training affects neuroplasticity and reward responses has been less considered, especially for subjects of Chinese origin. In this work, 16 musicians who trained in the Western music style (Western-trained musicians) and 18 musicians who trained in the Chinese music style (Chinese-trained musicians) were recruited as the musician group for the experiment, while 15 non-musicians were recruited as the control group. Using a paradigm that consisted of listening to Chinese and Western music and measurements using functional magnetic resonance imaging (fMRI) technology, we found that Chinese-trained musicians activated the bilateral superior temporal gyrus (STG) when listening to music, while Western-trained musicians activated the left STG. In addition, under the condition of listening to music with Chinese style, Chinese-trained musicians have a stronger functional connection in the circuit of the auditory and reward system than Western-trained musicians. The finding is opposite under the condition of listening to music with Western style. Interestingly, it seems that the circuit of Chinese-trained musicians is partial to the right STG, while Western-trained musicians show the opposite, i.e., a tendency toward the left STG. The influence of different music styles on experienced musicians is reflected by the functional activities and connections between the auditory system and the reward system. This outcome indicates that training in Chinese music style or Western music style affects the strategies of musicians when listening to music. Musical characteristics such as rhythm, melody and cultural attributes play an important role in this process. These findings, which provide evidence for functional neuroplasticity based on musical training, can enrich our insights into the musical brain.

18.
Front Genet ; 12: 682904, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34386039

RESUMO

Renal fibrosis (RF) is a pathological process that culminates in terminal renal failure in chronic kidney disease (CKD). Fibrosis contributes to progressive and irreversible decline in renal function. However, the molecular mechanisms involved in RF are complex and remain poorly understood. Long non-coding RNAs (lncRNAs) are a major type of non-coding RNAs, which significantly affect various disease processes, cellular homeostasis, and development through multiple mechanisms. Recent investigations have implicated aberrantly expressed lncRNA in RF development and progression, suggesting that lncRNAs play a crucial role in determining the clinical manifestation of RF. In this review, we comprehensively evaluated the recently published articles on lncRNAs in RF, discussed the potential application of lncRNAs as diagnostic and/or prognostic biomarkers, proposed therapeutic targets for treating RF-associated diseases and subsequent CKD transition, and highlight future research directions in the context of the role of lncRNAs in the development and treatment of RF.

19.
Mol Immunol ; 139: 87-96, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34461493

RESUMO

BACKGROUND: Kidney damage often develops into renal fibrosis. Apoptosis and inflammatory response are the main factors driving the process of renal fibrosis. Here we showed that lncRNA XIST/ miR-19b / SOX6 signal axis regulated apoptosis and inflammation of renal fibrosis. METHODS: HK-2 cells were treated with TGF-ß1 to construct cell fibrosis model, and UUO surgery was performed to construct mouse renal fibrosis model. The expression of XIST, miR-19b and SOX6 were examined by qPCR. And levels of fibrosis-related proteins were detected by western blotting. Levels of IL-1ß and TNF-α were assessed by qPCR and ELISA, respectively. Renal pathology and fibrosis were evaluated by HE and Masson staining. Flow cytometry and TUNEL staining were employed to evaluate cell apoptosis in cell fibrosis model and mouse renal fibrosis model, respectively. Besides, dual luciferase reporter assay was employed to verify whether XIST had a binding site to miR-19b, and whether miR-19b had a binding site to SOX6. RESULTS: Here we showed that XIST and SOX6 were upregulated in both HK-2 cells treatment of TGF-ß1 and kidneys of UUO mice, while miR-19b was downregulated. Dual luciferase reporter assay displayed that XIST directly bound to miR-19b, and SOX6 was the target of miR-19b. Knockdown of XIST inhibited apoptosis, inflammation and fibrosis in HK-2 cells treatment of TGF-ß1 via miR-19b-mediated downregulation of SOX6, while inhibition of miR-19b reversed the effect. Similarly, knockdown of XIST in vivo inhibited apoptosis, inflammation and fibrosis in kidneys of UUO mice via miR-19b-mediated downregulation of SOX6. DISCUSSION: These results provided evidence that knockdown of XIST inhibited apoptosis and inflammation of renal fibrosis via miR-19b-mediated downregulation of SOX6.

20.
Sci Total Environ ; 799: 149397, 2021 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-34371397

RESUMO

This study aimed to quantify the co-pyrolysis of textile dyeing sludge (TDS) and the two medical plastic wastes of syringes (SY) and medical bottles (MB) in terms of their performances, synergistic mechanisms, and products. The pyrolysis of polyolefin plastics with its high calorific value and low ash content can offset the poor mono-pyrolytic performance of TDS. The synergistic mechanisms occurred mainly in the range of 400-550 °C. The addition of 10% SY or MB achieved the best co-pyrolysis performance with the lowest activation energy. The co-pyrolysis increased the contents of CH4 and CH but reduced CO2 emission. The co-pyrolysis released more fatty hydrocarbons, alcohols, and cyclic hydrocarbon during but reduced the yields of ethers and furans, through the synergistic mechanisms. The addition of the polyolefin plastics made the micro surface particles of chars smaller and looser. Our results can benefit energy utilization, pollution control, and optimal operational conditions for the industrial thermochemical conversions of hazardous wastes.


Assuntos
Resíduos de Serviços de Saúde , Pirólise , Plásticos , Esgotos , Têxteis
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