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1.
Adv Exp Med Biol ; 1240: 35-46, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32060886

RESUMO

T cells can be categorized into functionally diverse subpopulations, which include Th1, Th2, Th9, Th17, Th22, and Tfh cells and Foxp3+ Tregs, based on their role in maintaining normal immune homeostasis and affecting pathological immune-associated diseases. Among these subpopulations, Th9 cells are relatively new, and less is known about their signaling and effects on tumor immunity. Recently, some studies have focused on regulation of the IL-9/IL-9R signaling pathway and Th9 cell differentiation and their roles in tumor environments. Herein, we summarize recent progress in understanding the regulatory signaling of IL-9 and Th9 cells and their critical roles and mechanisms in antitumor immunity.

2.
J Psychiatr Res ; 123: 136-144, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32065949

RESUMO

The human gut microbiome plays an important role in the basic neurodevelopmental processes of the central nervous system and has been implicated in several neuropsychiatric disorders. However, the connection between the gut microbiome and the underlying pathogenesis of schizophrenia (SCZ) is poorly defined. Here we analyzed the faecal samples from 40 first-episode drug-naïve SCZ (FSCZ) patients, 85 chronically antipsychotic-treated SCZ (TSCZ) patients and 69 healthy controls (HCs) using 16S rRNA gene sequence to determine whether the alterations of the gut microbiome were associated with SCZ or antipsychotic treatment. In addition, we acquired the T1-weighted brain imaging data by using structural magnetic resonance imaging to test whether microbial composition correlated with structural brain signatures. Our analyses revealed low microbiome alpha-diversity indexes in TSCZ patients but not in FSCZ patients as compared to HCs. Importantly, both FSCZ and TSCZ patients had distinct changes in gut microbial composition at certain taxa including Christensenellaceae, Enterobacteriaceae, Pasteurellaceae, Turicibacteraceae at the family level and Escherichia at genus level as compared to HCs. We also found significant disturbances of gut microbial composition in TSCZ versus FSCZ patients (eg. Enterococcaceae and Lactobacillaceae). Most interestingly, our exploratory analyses found specific SCZ-associated microbiota to be correlated with the right middle frontal gyrus (rMFG) volume which was aberrant in SCZ patients. Our findings extend prior work and suggest a possible link between the gut microbiome and brain structure which may be implicated in the pathology of SCZ.

3.
Chem Asian J ; 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-32017419

RESUMO

The reverse-binding-selectivity between monovalent and divalent cations was observed for two different self-assembly G16-hexadecamer and G8-octamer systems.  The dissociation constant between G4-quadruplex and monomer was calculated via VT-1H NMR experiments.  Quantitative energy profiles revealed entropy as the key factor for the weaker binding toward Ba2+ compared with K+ in the G8-octamer system despite stronger ion-dipole interactions.  This study is the first direct comparison of G4-quartet binding affinity between mono and divalent cations and will benefit future applications of G-quadruplexes related research.  Further competition experiments between G8-octamer and 18-crown-6 with K+ demonstrated the potential of this G8 system as a new potassium receptor.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32031697

RESUMO

PURPOSE: A previous study reported a novel c.544_618del75bp mutation in exon 7 of the PRPF31 gene in a Chinese family with autosomal dominant retinal pigmentosa (ADRP). However, the selected pedigree was a small part of the whole family and the function of the c.544_618del75bp mutation was not explored deeply. The aim of the present study was to validate the previous results and explore the functional significance of the c.544_618del75bp mutation. METHODS: We extended the size of the ADRP pedigree and sequenced DNA and cDNA of the PRPF31 gene for all members of the family and 100 healthy controls. Real-time quantitative polymerase chain reaction (PCR) analysis was performed on the cDNA of patients in the family and cell culture, plasmids transfection and western blot analysis were done to evaluate the functional effect of the mutation in vitro. RESULTS: Sanger sequencing showed that the mutation was present in all patients and absent in all normal individuals, except for participant III-9. Bioinformatics analysis revealed that the c.544_618del75bp mutation caused a 25 amino acid deletion in the PRPF31 protein. In addition, the mRNA expression assay revealed that the mRNA expression level of the PRPF31 and RP9 genes were significantly lower in RP patients than controls (p < 0.05). Finally, the in vitro transfection assay demonstrated that the mRNA expression level of the mutant transfection group was significantly lower than the wild-type transfection group (p < 0.05). CONCLUSIONS: Our study suggested that the c.544_618del75bp mutation in the PRPF31 gene was a causative mutation in this ADRP family and affected the expression of RP9 gene by influencing the formation of U4/U6-U5 tri-snRNP, eventually leading to the occurrence of RP.

5.
Spectrochim Acta A Mol Biomol Spectrosc ; 230: 118031, 2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-31931357

RESUMO

Fluorescent and robust fibers based on gold nanoclusters-loaded alginate were successfully prepared by wet spinning of gold nanoclusters and alginate. The relationship between process conditions, mechanical properties, and fluorescent properties of fibers was investigated. The as-prepared fibers exhibited high mechanical strength (up to 7.09 cN/dtex) and remarkable red emission under ultraviolet excitation. The fibers could be used as a simple, low-cost, and high-selectivity fluorescent sensor for detecting Cu2+ and Hg2+ among various metal ions in aqueous solution, with a detection limit as low as 187.99 nM for Cu2+ and 82.14 nM for Hg2+, respectively. Furthermore, the novel fluorescent fibers were used as an anti-counterfeiting label through knitting into textile materials. The wet-spun functional fibers may be applied to the design of smart wearable sensors and flexible optical sensors.

6.
Endocr Pract ; 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-31968198

RESUMO

Objective: Early diagnosis and treatment of children with congenital hypothyroidism (CH) through newborn screening can effectively prevent delayed development in children. This study was designed to investigate the pathogenesis and influencing factors of CH in urban areas of China between 2009 and 2018. Methods: A retrospective analysis of newborn screening data and diagnosis and treatment information for CH diagnosed in the information database of the neonatal disease screening center in one of China's five special economic zones from 2009 to 2018. Results: Of the 947,258 newborns screened between 2009 and 2018, 829 (406 girls) were diagnosed with CH at birth(1/1136). Among the 608 cases of CH diagnosed at birth and reevaluated at the age of 3 years, 487 were perma(PCH, 1/1429) and 121 were transient (TCH, 1/5882).83.2% of infants with PCH (405/487) underwent thyroid imaging in the neonatal period, of which thyroid dysgenesis accounted for 28.64% (116/405) and functional defects accounted for 71.36% (289/405). The incidence of CH changed significantly in infants with initial serum TSH concentrations of 41-100 mIU/L and ≥100 mIU/L, while the incidence of mild CH showed a slight increase. The incidence of CH was significantly higher in post-term infants (1/63) and low birth weight infants (1/370). Conclusions: In the past decade, the incidence of CH has increased, mainly due to the increase in the incidence of PCH and TCH. The incidence of mild CH has increased slightly. Post-term birth and low birth weight are important factors affecting the incidence of CH.

7.
Biomolecules ; 10(2)2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31979323

RESUMO

Various ferulic acid (FA) dimers occurring in plant cell walls, such as 8-5-, 8-O-4-, 5-5-, and 8-8- coupled dimers, are effective antioxidants and potential antimicrobials. It is necessary to access these diferulates as reference compounds to validate those isolated from plants. 3,6-bis(4-hydroxy-3-methoxyphenyl)-tetrahydrofuro-[3,4-c]furan-1,4-dione, a 8-8-coupled FA dilactone generated from ferulic acid via radical coupling, has been used to synthesize 8-8-coupled FA dimers although few reports investigated the distribution of products and mechanisms involved in the transformation of FA dilactone. In this work, the FA dilactone, obtained from FA by a peroxidase-catalyzed radical coupling, was reacted under various base/acid conditions. Effects of reaction conditions and workup procedures on the distribution of products were investigated by GC-MS. The isolated products from such treatments of FA dilactone were characterized by NMR. New derivatives of FA dimer including 2-(4-hydroxy-3-methoxybenzylidene)-3-(hydroxyl-(4-hydroxy-3-methoxyphenyl)methyl)succinic acid and 2-(bis(4-hydroxy-3-methoxyphenyl)-methyl)-succinic acid were produced from NaOH treatment. Another novel 8-8-coupled cyclic FA dimer, diethyl 6-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-methoxy-1,2-dihydronaphthalene-2,3-dicarboxylate was identified in products from FA dilactone treated by dry HCl in absolute ethanol. Mechanisms involved in such transformations were proposed.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31928494

RESUMO

Background: This study aimed to explore the clinical efficacy of three-dimensional (3D) laparoscopic surgery in the treatment of congenital choledochal cysts (CCCs) by comparing it with two-dimensional (2D) laparoscopic surgery. Patients and Methods: We retrospectively reviewed data of 155 pediatric patients who underwent surgical treatment of choledochal cysts between January 2014 and December 2017. We divided the patients into two groups according to the surgical method used-a 3D laparoscopic group (N = 42) and a 2D laparoscopic group (N = 113). The 3D laparoscopic group was further divided into two subgroups based on age-Group 1 (age ≤12 months, N = 11) and Group 2 (age >12 months, N = 31). We analyzed data in terms of the following characteristics: patient demographics, perioperative and follow-up conditions, and complications. Moreover, we also recorded and analyzed the surgeon's assessment on laparoscopic system usage. Results: Cyst excision and Roux-en-Y hepaticojejunostomy (HJ) was successfully completed in all the patients. Intergroup differences in operating time and blood loss were statistically significant. There were no significant differences between the two groups in early and late complications, such as the incidence of wound infection, HJ stricture, or adhesive ileus. There were no significant differences in the operative data and outcomes between the two subgroups of patients who underwent 3D laparoscopic treatment. Based on surgeon's assessment, the 3D laparoscopic system had better depth perception and accuracy than the 2D laparoscopic system; however, there was no difference in the adverse effect on surgeons. Conclusions: Compared with the traditional 2D laparoscopic surgery, 3D laparoscopic surgical resection of CCCs combined with jejunum Roux-en-Y anastomosis is a safer and more effective procedure that can shorten operative time and reduce intraoperative bleeding with no increase in surgical strain. The 3D laparoscopic surgery technique may provide a better choice for CCC operations.

9.
Aging (Albany NY) ; 12(2): 1577-1590, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31980592

RESUMO

Nearly half of metastatic breast cancers (MBC) have genetic aberrations in the PI3K/AKT pathway. To investigate the distinct effect of these aberrations on MBC, 193 MBC patients who progressed after the early line (≤2) salvage treatment voluntarily received next generation sequencing (NGS) for a panel of 1,021 genes. 93 (48%) patients had genetic aberrations in the PI3K/AKT pathway. The number of patients with PIK3CA mutations in kinase domain (KD), helical domain (HD) and other domain (OD), were 36 (18.7%), 26 (13.5%), 10 (5.2%), respectively. 21 (10.9%) patients had mutations in PI3K/AKT pathway genes other than PIK3CA (P/A). Compared to PI3K/AKT-wild type (WT) patients, PIK3CA-HD patients had a significantly shorter progression-free survival (PFS) (Logrank p-value < 0.0001). PIK3CA-KD, PIK3CA-OD and other P/A mutations showed similar PFS to WT patients (Logrank p-value = 0.63). PIK3CA-HD patients had a distinct ctDNA mutation profile to patients with other PI3K/AKT mutations. PIK3CA-HD patients had a higher rate of FGFR and NF1 aberrations. In addition, more PIK3CA-HD carriers were TMB-high. Cox regression analyses suggested that PIK3CA-HD mutations, FGFR aberrations and high TMB were all significant risk factors for poor PFS. In conclusion, future research needs to focus more on the treatment strategies targeting PIK3CA-HD mutations.

10.
Exp Mol Pathol ; 113: 104371, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31917290

RESUMO

As the number of elderly patients increases, some patients with heart problems may also need surgery. The purpose of this study was to investigate whether dexmedetomidine (DEX), a common used anesthetic, was beneficial to the patients with heart problems. Myocardial cells induced by doxorubicin (DOX) was to simulate the myocardium injury in vitro. H9c2 cells were treated with DOX, DEX/DOX, Compound C and Compound C/DEX/DOX, respectively. The expression of p-AMPK, AMPK, p-GSK3ß, GSK3ß, Bcl2, Bax, Cleaved caspase3, Caspase3, TXNIP, NLRP3, ASC, Cleaved caspase-1 and Caspase-1 were analyzed by Western blot. CCK-8 assay and flow cytometry analysis were used to detect the cell viability and cell apoptosis. The levels of TNF-α, IL-1ß and IL-18 were detected by ELISA assay and the levels of NO, ROS, LDH, SOD, MDA and taurine were detected by corresponding assay kits. As a result, DEX promoted the cell viability and inhibited the inflammation, oxidative stress and apoptosis. In addition, DEX suppressed the expression of taurine, TXNIP, NLRP3, ASC and cleaved caspase-1 and activated the expression of p-AMPK and p-GSK3ß. However, those above changes could be reversed by Compound C. In conclusion, this study indicated that DEX could reduce the inflammation and apoptosis of DOX-induced myocardial cells through activating the AMPK-GSK3ß signaling pathway. Because of the above effects of DEX, it may be beneficial for surgical patients with heart problems.

11.
IEEE Trans Vis Comput Graph ; 26(2): 1361-1371, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30176598

RESUMO

This paper presents Poisson vector graphics (PVG), an extension of the popular diffusion curves (DC), for generating smooth-shaded images. Armed with two new types of primitives, called Poisson curves and Poisson regions, PVG can easily produce photorealistic effects such as specular highlights, core shadows, translucency and halos. Within the PVG framework, the users specify color as the Dirichlet boundary condition of diffusion curves and control tone by offsetting the Laplacian of colors, where both controls are simply done by mouse click and slider dragging. PVG distinguishes itself from other diffusion based vector graphics for 3 unique features: 1) explicit separation of colors and tones, which follows the basic drawing principle and eases editing; 2) native support of seamless cloning in the sense that PCs and PRs can automatically fit into the target background; and 3) allowed intersecting primitives (except for DC-DC intersection) so that users can create layers. Through extensive experiments and a preliminary user study, we demonstrate that PVG is a simple yet powerful authoring tool that can produce photo-realistic vector graphics from scratch.

12.
Biosens Bioelectron ; 150: 111898, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31767347

RESUMO

Poly [(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1',3}-thiadazole)] (PFBT) was functionalized with carboxyl groups via nanoprecipitation method to prepare polymer nanoparticles (PNPs). Remarkable electrochemiluminescence (ECL) was observed at PFBT PNPs without any external species or dissolved O2 as coreactants. Furthermore, such an ECL emission could be efficiently quenched by hydrogen peroxide (H2O2). A coreactant-free ECL enzyme-based biosensor is making use of PFBT PNPs as luminophore for detecting organophosphorus (OPs) pesticides. In the absence of OPs, H2O2 stemmed from the enzymatic reaction would quench the ECL signal of PFBT PNPs, resulting in an ECL signal-off state. In the presence of OPs, the ECL signal obviously increased because the enzyme activity of the acetylcholinesterase (AChE) was inhibited by OPs. The linear range for OPs detection was 1.0 × 10-12-1.0 × 10-7 M and the detection limit was low as 1.5 × 10-13 M. Such a coreactant-free ECL strategy could avoid the drawbacks resulted from the addition of exogenous species or dissolved O2 as coreactant. More importantly, H2O2 was released by many oxidases-induced reactions, and its quenching effect on the ECL of PFBT PNPs would pave a new avenue for constructing coreactant-free enzyme-based ECL biosensor for environmental monitoring and biological analysis.

13.
Am J Reprod Immunol ; 83(2): e13202, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31646704

RESUMO

PROBLEM: The complement system plays a key role in normal placentation, and delicate regulation of complement system activation is critical for successful pregnancy. Therefore, establishing a normal range of complement components during pregnancy is important for clinical evaluation and research. METHODS: We performed a prospective study to investigate the normal range of complement components in circulation during different stages of pregnancy. Plasma concentrations of complement factor B (CFB), C1q, complement factor H (CFH), C3, C3c, and C4 were measured using an immunoturbidimetric assay; mannan-binding lectin (MBL), C3a, C5a, and soluble C5b-9 (sC5b-9) levels at different time points of pregnancy were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: A total of 733 plasma samples were collected from 362 women with a normal pregnancy and 65 samples from non-pregnant women. In the first trimester of pregnancy, the levels of CFB, CFH, MBL, C3c, C4, and C3a were 414.5 ± 85.9 mg/L (95% CI for mean: 402.4-426.6 mg/L), 381.0 ± 89.0 mg/L (95% CI for mean: 368.5-393.6 mg/L), 4274.5 ± 2752 ng/mL (95% CI for mean: 3881.1-4656.4 ng/mL), 1346.9 ± 419.8 mg/L (95% CI for mean: 1287.7-1406.0 mg/L), 357.4 ± 101.8 mg/L (95% CI for mean: 343.0-371.7 mg/L), and 182.5 ± 150.0 ng/mL (95% CI for mean: 186.9-229.1 ng/mL), respectively. The levels of C3 and C4 increased gradually throughout pregnancy. The levels of C1q, C5a, and sC5b-9 in the first and second trimesters were nearly the same as those in non-pregnant women. CONCLUSION: The results of this study show that pregnancy itself may influence the plasma levels of complement system components.

14.
Environ Pollut ; 257: 113489, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31706773

RESUMO

Ammonia emission is one of the dominant pathways of nitrogen fertilizer loss from rice fields in China. It is difficult to measure ammonia emissions by high-frequency sampling with the chamber methods widely used in China, which is of great significance for investigating the environmental effects on the ammonia emissions. The chamber methods also can not accurately determine the ammonia emissions. In this study, the backward Lagrangian stochastic dispersion model, with ammonia concentrations continuously measured by the open-path tunable diode laser absorption spectroscopy technique, was used to determine ammonia emissions from a rice field after fertilizer application at excessive (270 kg N ha-1) and appropriate (210 kg N ha-1) rates in the Taihu Lake Region of China. High temporal resolution measurements of ammonia emissions revealed that high intraday fluctuations of ammonia emissions were significantly affected by the meteorological conditions. Multiple regression analysis showed a dominant solar radiation dependence of intraday ammonia emission cycles, especially during the rice panicle formation stage. The NH4+-N concentrations of the surface water of the rice field were found to be the decisive factor that influenced interday dynamics of ammonia emissions. Accurate quantifications of ammonia emissions indicated that the total ammonia losses under appropriate nitrogen application rate were 27.4 kg N ha-1 during the rice tillering stage and 11.2 kg N ha-1 during the panicle formation stage, which were 29.4% and 17.0% less than those under traditional excessive nitrogen application rate used by the local farmers, respectively. The ammonia loss proportions during the rice panicle formation stage were significantly lower than those of the tillering stage, which might be due to different nitrogen application rates and environmental effects during the two stages. This study indicated that the open-path tunable diode laser absorption spectroscopy technique could facilitate the investigation of high temporal resolution dynamic of ammonia emissions from farmland and the environmental influence on the ammonia emissions.

15.
Anat Histol Embryol ; 49(1): 31-37, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31571240

RESUMO

The distribution of glucagon-like peptide 1 (GLP-1)-positive cells in digestive tracts and pancreases of aquatic vertebrates was investigated by immunohistochemical staining method. The results suggested that GLP-1-positive cells were distributed in the columnar mucous epithelium and tubular glands of lamina propria in the digestive system. However, GLP-1-positive cells were also found in subepithelial lamina propria of the mucosae and muscularis in each segment of the digestive tract of Rana nigromaculata. The distribution densities of these cells reached peaks in the stomachs, and the middle or end segments of small intestines of Chinese softshell turtle, Bufo gargarizans, R. nigromaculata and catfish, and there was the third distribution density peak in the rectum of catfish. The total amount or overall density of GLP-1-positive cells varied a lot in the digestive tracts of different animal species. The distribution density was relatively low in the digestive tract of chub and reached the maximum in the digestive tracts of snakehead and catfish, but no GLP-1-positive cells were found in the digestive tract of bighead carp. GLP-1-positive cells were densely distributed in the pancreases of Chinese softshell turtle, B. gargarizans and R. nigromaculata. These cells spread over the superficial layers of islets or scattered in exocrine pancreas in the pancreas of B. gargarizans, spread in the endocrine cells or scattered in the pancreas of Chinese softshell turtle, scattered in the pancreas of R. nigromaculata and distributed in the superficial layers of islets in the pancreas of catfish.

16.
J Oral Rehabil ; 47(1): 1-8, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31378989

RESUMO

BACKGROUND: Oro-facial function is usually impaired by temporomandibular disorders (TMDs). Several studies on TMDs have used the Jaw Functional Limitation Scale (JFLS) to assess mandibular dysfunction. However, it was originally created in English and hence needs to be validated for use among Chinese people. OBJECTIVE: To develop a Chinese version of the JFLS for Chinese TMD patients and to investigate the validity and reliability of the scale. METHODS: Content validity and temporal stability were evaluated at two different occasions. The reliability and validity of the JFLS were tested in 483 TMD patients. Cronbach's alpha coefficient and split-half reliability were used to assess internal consistency, while the validity was evaluated by factor analysis. RESULTS: Three factors were extracted during exploratory factor analysis, accounting for 62.39% of the variance. The three-factor model was then measured using confirmatory factor analysis (χ2 /df = 3.6, root mean square error of approximation = 0.091, comparative fit index = 0.896). Internal (coefficient alpha values of .906 for all items and Guttman split-half reliability of 0.756) and test-retest (intra-class correlation coefficient = .851-.897, 95% confidence interval = 0.656-0.950) reliabilities were excellent. CONCLUSION: The Chinese version of the JFLS is reliable and valid for use in Chinese TMD patients.


Assuntos
Transtornos da Articulação Temporomandibular , Humanos , Arcada Osseodentária , Psicometria , Reprodutibilidade dos Testes , Inquéritos e Questionários
17.
Support Care Cancer ; 28(1): 373-380, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31049672

RESUMO

BACKGROUND/OBJECTIVES: The assessment of nutritional status and the quality of life in patients with gastric cancer has become one of the important goals of current clinical treatment. The purpose of this study was to assess the nutritional status in hospitalized gastric cancer patients by using patient-generated subjective global assessment (PG-SGA) and to analyze the influence of nutritional status on the patients' quality of life (QOL). METHODS: We reviewed the pathological diagnosis of gastric cancer for 2322 hospitalized patients using PG-SGA to assess their nutritional status and collected data on clinical symptoms, the anthropometric parameters (height, weight, body mass index (BMI), mid-arm circumference (MAC), triceps skin-fold thickness (TSF), and hand-grip strength (HGS). We also collected laboratory data (prealbumin, albumin, hemoglobin) within 48 h after the patient was admitted to the hospital. The 30-item European Organization for Research and Treatment of Cancer Core Quality of Life Questionnaire (EORTC QLQ-C30) was used for QOL assessment in all patients. RESULTS: By using PG-SGA, we found 80.4% of the patients were malnourished (score ≥ 4) and 45.1% of the patients required urgent nutritional support (score ≥ 9). In univariate analysis, old age (> 65 years, p < 0.001), female (p = 0.007), residence in a village (p = 0.004), a lower level of education (p < 0.001), and self-paying (p < 0.001) were indicated as risk factors of patients with gastric cancer to be suffering from severe malnutrition. There was a negative correlation between PG-SGA and various nutritional parameters (p < 0.05). The quality of life was significantly different in gastric cancer patients with different nutritional status (p < 0.01). CONCLUSION: Malnutrition of hospitalized patients with gastric cancer in China is common and seriously affects the patients' quality of life. The nutritional status should be evaluated in a timely manner and reasonable nutritional intervention should be provided as soon as possible. The PG-SGA was fit for using as a clinical nutrition assessment method, being worthy of clinical application.

18.
Chem Commun (Camb) ; 56(4): 663, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-31848552

RESUMO

Correction for 'Enantioselective iridium catalyzed α-alkylation of azlactones by a tandem asymmetric allylic alkylation/aza-Cope rearrangement' by Xue-Dan Bai et al., Chem. Commun., 2019, 55, 5547-5550.

20.
Infect Immun ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31818961

RESUMO

Revealing the mechanisms by which bacteria establish long-lasting colonization in the gastrointestinal tract is an area of intensive investigation. The obligate intracellular bacterium Chlamydia is known to colonize mouse colon for long periods of time. A colonization-deficient mutant strain of this intracellular bacterium is able to regain long-lasting colonization in IFNγ knockout mice following an intracolon inoculation. We now report that mice deficient in conventional T lymphocytes or recombination activating gene (Rag) failed to rescue the mutant colonization. Nevertheless, antibody depletion of IFNγ or genetic deletion of IL-2 receptor common gamma chain in Rag deficient mice did rescue mutant colonization. These observations suggest that colonic IFNγ responsible for inhibiting the intracellular bacterium mutant is produced by innate lymphoid cells (ILCs). Consistently, depletion of NK1.1+ cells in Rag deficient mice both prevented IFNγ production and rescued the bacterium mutant colonization. Furthermore, mice deficient in transcriptional factor RORγt, but not chemokine receptor CCR6, fully rescued the long-lasting colonization of the mutant, indicating that the role of group 3-like ILCs. However, the inhibitory function of the responsible group 3-like ILCs was not dependent on the natural killer cell receptor (NCR1) since NCR1-deficient mice still inhibited the mutant colonization. Consistently, mice deficient in the transcriptional factor T-bet only delayed the clearance of the bacterial mutant without fully rescuing the long-lasting colonization of the mutant. Thus, we have demonstrated that the obligate intracellular bacterium Chlamydia maintains its long-lasting colonization in the colon by evading IFNγ from group 3-like ILCs.

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