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1.
Food Res Int ; 147: 110493, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34399489

RESUMO

Pungency is increasingly being recognized as an important factor of overall sensory quality, palatability, and consumer preference of distilled spirits. The characterization of pungency is necessary to evaluate the potential sensory quality of distilled spirits. In this study, the temporal profiles of pungency of Baijiu with different aging times were evaluated using time-intensity (TI) and temporal dominance of sensations (TDS) methods, considering both pungency intensity and pungency sub-qualities. TI results indicated significant differences in release rate of pungency during Baijiu consumption. Compared to young Baijiu, old Baijiu tend to show higher release rate of pungency, the areas under the curve and duration of pungency were significantly decreased in old Baijiu. The TDS results showed significant differences in the combination of dominant sub-qualities, as well as in the maximum dominance rates and the dominant duration of sub-qualities among Baijiu. The young Baijiu were mainly characterized by the dominant sub-qualities of "burning" and "numbing", whereas for old Baijiu, "burning", "prickle", and "drying" were dominant. The application of TI and TDS provided dynamic and temporal profiles of pungency to fully characterize pungency differences of distilled beverages.


Assuntos
Sensação , Paladar , Bebidas , Comportamento do Consumidor
2.
J Med Microbiol ; 70(3)2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33591245

RESUMO

Introduction. Shigella sonnei, the cause of bacillary dysentery, belongs to Gram-negative enteropathogenic bacteria. S. sonnei contains a 210 kb virulence plasmid that encodes an O-antigen gene cluster of LPSs. However, this virulence plasmid is frequently lost during replication. It is well-documented that after losing the O-antigen and becoming rough strains, the Gram-negative bacteria may express an LPS core on its surface. Previous studies have suggested that by using the LPS core, Gram-negative bacteria can interact with several C-type lectin receptors that are expressed on antigen-presenting cells (APCs).Hypothesis/Gap Statement. S. sonnei by losing the virulence plasmid may hijack APCs via the interactions of LPS-CD209/CD207.Aim. This study aimed to investigate if the S. sonnei rough strain, by losing the virulence plasmid, interacted with APCs that express C-type lectins of human CD207, human CD209a and mouse CD209b.Methodology. SDS-PAGE silver staining was used to examine the O-antigen expression of S. sonnei WT and its rough strain. Invasion assays and inhibition assays were used to examine the ability of S. sonnei WT and its rough strain to invade APCs and investigate whether CD209 and CD207 are receptors for phagocytosis of rough S. sonnei. Animal assays were used to observe the dissemination of S. sonnei.Results. S. sonnei did not express O-antigens after losing the virulence plasmid. The S. sonnei rough strain invades with APCs, including human dendritic cells (DCs) and mouse macrophages. CD209 and CD207 are receptors for phagocytosis of rough S. sonnei. Expression of the O-antigen reduces the ability of the S. sonnei rough strain to be disseminated to mesenteric lymph nodes and spleens.Conclusion. This work demonstrated that S. sonnei rough strains - by losing the virulence plasmid - invaded APCs through interactions with CD209 and CD207 receptors.


Assuntos
Antígenos CD/imunologia , Moléculas de Adesão Celular/imunologia , Disenteria Bacilar/microbiologia , Lectinas Tipo C/imunologia , Lectinas de Ligação a Manose/imunologia , Antígenos O , Plasmídeos , Receptores de Superfície Celular/imunologia , Shigella sonnei/patogenicidade , Virulência/genética , Animais , Células CHO , Cricetulus , Células Dendríticas/microbiologia , Interações Hospedeiro-Patógeno , Humanos , Macrófagos/microbiologia , Camundongos , Antígenos O/genética , Antígenos O/metabolismo , Shigella sonnei/genética
3.
Bosn J Basic Med Sci ; 21(1): 71-80, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31999938

RESUMO

Endothelial progenitor cell (EPC) transplantation is a safe and effective method to treat acute myocardial infarction (AMI). However, oxidative stress leads to the death of a large number of EPCs in the early stage of transplantation, severely weakening the therapeutic effect. Previous studies demonstrated that microRNAs regulate the biological function of EPCs. The aim of the current study was to investigate the effect of microRNA on the biological function of EPCs under oxidative stress. Quantitative reverse transcription PCR was performed to detect the expression of miR-126, miR-508-5p, miR-150, and miR-16 in EPCs from rats, among which miR-126 showed a relatively higher expression. Treatment with H2O2 decreased miR-126 expression in EPCs in a dose-dependent manner. EPCs were further transfected with miR-126 mimics or inhibitors, followed by H2O2 treatment. Overexpression of miR-126 enhanced the proliferation, migration, and tube formation of H2O2-treated EPCs. MiR-126 overexpression also inhibited reactive oxygen species and malondialdehyde levels and enhanced superoxide dismutase levels, as well as increased angiopoietin (Ang)1 expression and decreased Ang2 expression in H2O2-treated EPCs. Moreover, miR-126 participated in the regulation of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/glycogen synthase kinase 3ß (GSK3ß) and extracellular signal-regulated kinase 1/2 (ERK1/2) signaling in EPCs, where both pathways were activated after miR-126 overexpression in H2O2-treated EPCs. Overall, we showed that miR-126 promoted the biological function of EPCs under H2O2-induced oxidative stress by activating the PI3K/Akt/GSK3ß and ERK1/2 signaling pathway, which may serve as a new therapeutic approach to treat AMI.

4.
Food Chem ; 331: 127335, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32574944

RESUMO

Comprehensive 2D gas chromatography-time-of-flight mass spectrometry was combined with descriptive sensory analysis to elucidate the specificity of strong-aroma type Baijiu (Chinese liquor) from different regions, based on regionally distinct flavor characterized by chemical and sensory profiles. Numerous potential aroma compounds (262) were identified, among which 58 aroma compounds were significantly different between the samples from Sichuan and Jianghuai regions. Relationships between these potential aroma compounds and sensory attributes were investigated by partial least squares regression and network analysis. The compounds that dominantly contributed to the important sensory attributes were identified. The high pyrazines, furanoids, and carbonyls amounts contributed to the high intensities of the cellar, toasted, and grain aroma profiles of the Sichuan region samples, while the high ester and alcohol levels contributed to the fruity and floral aroma profiles of the Jianghuai region samples. This approach may have practical application in flavor characterization of other alcoholic beverages.


Assuntos
Bebidas Alcoólicas/análise , Análise de Alimentos/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Odorantes/análise , Compostos Orgânicos Voláteis/análise , China , Ésteres/análise , Análise de Alimentos/estatística & dados numéricos , Cromatografia Gasosa-Espectrometria de Massas/estatística & dados numéricos , Humanos , Análise dos Mínimos Quadrados , Análise Multivariada , Pirazinas/análise , Paladar
5.
J Recept Signal Transduct Res ; 40(2): 126-132, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32003295

RESUMO

Chlamydia pneumonia (C.pn) is a common respiratory pathogen that is involved in human cardiovascular diseases and promotes the development of atherosclerosis in hyperlipidemic animal models. C.pn reportedly up-regulated lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in endothelial cells. Recently, the anti-atherosclerotic activity of peroxisome proliferator-activated receptor γ (PPARγ) has been documented. In the present study, we investigated the effect of C.pn on LOX-1 expression in human umbilical vein endothelial cells (HUVECs) and identified the involvement of the PPARγ signaling pathway therein. The results showed that C.pn increased the expression of LOX-1 in HUVECs in a dose- and time-dependent manner. C.pn-induced up-regulation of LOX-1 was mediated by ERK1/2, whereas p38 MAPK and JNK had no effect on this process. C.pn induced apoptosis, inhibited cell proliferation, and decreased the expression PPARγ in HUVECs. Additionally, LOX-1 activity and cell injury caused by C.pn through activation of ERK1/2 was completely inhibited by rosiglitazone, a PPARγ agonist. In conclusion, we inferred that activation of PPARγ in HUVECs suppressed C.pn-induced LOX-1 expression and cell damage by inhibiting ERK1/2 signaling.


Assuntos
Aterosclerose/genética , Doenças Cardiovasculares/genética , PPAR gama/genética , Receptores Depuradores Classe E/genética , Apoptose/genética , Aterosclerose/microbiologia , Aterosclerose/patologia , Doenças Cardiovasculares/microbiologia , Doenças Cardiovasculares/patologia , Proliferação de Células/genética , Chlamydophila pneumoniae/genética , Chlamydophila pneumoniae/patogenicidade , Regulação da Expressão Gênica/genética , Células Endoteliais da Veia Umbilical Humana/microbiologia , Humanos , Sistema de Sinalização das MAP Quinases/genética , PPAR gama/agonistas , Rosiglitazona/farmacologia , Transdução de Sinais/efeitos dos fármacos , Veias Umbilicais/metabolismo , Veias Umbilicais/patologia , Proteínas Quinases p38 Ativadas por Mitógeno/genética
6.
Microb Pathog ; 141: 103993, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31988008

RESUMO

Yersinia pestis, a Gram-negative bacterium, is the etiologic agent of plague. A hallmark of Y. pestis infection is the organism's ability to rapidly disseminate through an animal host. Y. pestis expresses the outer membrane protein, Ail (Attachment invasion locus), which is associated with host invasion and serum resistance. However, whether Ail plays a role in host dissemination remains unclear. In this study, C57BL/6J mice were challenged with a defined Y. pestis strain, KimD27, or an isogenic ail-deleted mutant derived from KimD27 via metacarpal paw pad inoculation, nasal drops, orogastric infection, or tail vein injection to mimic bubonic, pneumonic, oral, or septicemic plague, respectively. Our results showed that ail-deleted Y. pestis KimD27 lost the ability to invade host cells, leading to failed host dissemination in the pneumonic and oral plague models but not in the bubonic or septicemic plague models, which do not require invasiveness. Therefore, this study demonstrated that whether Ail plays a role in Y. pestis pathogenesis depends on the infection route.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Peste/microbiologia , Fatores de Virulência/metabolismo , Virulência , Yersinia pestis , Animais , Proteínas de Bactérias/metabolismo , Modelos Animais de Doenças , Pulmão/microbiologia , Camundongos , Camundongos Endogâmicos C57BL , Boca/microbiologia , Yersinia pestis/metabolismo , Yersinia pestis/patogenicidade
7.
JBI Database System Rev Implement Rep ; 17(5): 985-1002, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31090653

RESUMO

INTRODUCTION: Asthma is one of the most common diseases in children. A self-management program can effectively improve the outcomes for children with asthma and reduce the burden on healthcare services. OBJECTIVES: The aim of this project was to integrate the best evidence on asthma self-management with practice in a children's respiratory clinic and to improve compliance with best practice. METHODS: Seven audit criteria were developed for the pre- and post-audit based on the best available evidence. The Joanna Briggs Institute (JBI) Practical Application of Clinical Evidence System and Getting Research into Practice audit and feedback tools were used in this project. RESULTS: The baseline audit showed a gap between clinical practice and the best evidence. The only criterion that achieved high compliance was provision of inhaler guidance (100%). After implementation, there were substantial improvements in compliance for many criteria. Training of clinicians increased from 13% at baseline to 67% at follow-up. Education of parents improved, with specific education about asthma triggers increasing from 55% to 100%, education about warning signs from 30% to 85% and education about effective asthma treatment options from 40% to 85%. Use of written asthma action plans increased from 0% to 25%. CONCLUSION: Strategies developed in this project were effective at providing necessary information for parents and improved the compliance with evidence. Further implementation strategies and audits are still needed to improve the use of asthma action plans and ensure they are reviewed periodically.


Assuntos
Asma/terapia , Prática Clínica Baseada em Evidências , Pessoal de Saúde/educação , Pais/educação , Autogestão , Criança , Conhecimentos, Atitudes e Prática em Saúde , Hospitalização , Humanos , Monitorização Fisiológica , Nebulizadores e Vaporizadores
8.
Infect Immun ; 87(8)2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31085704

RESUMO

Salmonella enterica serovar Typhimurium, a Gram-negative bacterium, can cause infectious diseases ranging from gastroenteritis to systemic dissemination and infection. However, the molecular mechanisms underlying this bacterial dissemination have yet to be elucidated. A study indicated that using the lipopolysaccharide (LPS) core as a ligand, S Typhimurium was able to bind human dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (hCD209a), an HIV receptor that promotes viral dissemination by hijacking antigen-presenting cells (APCs). In this study, we showed that S Typhimurium interacted with CD209s, leading to the invasion of APCs and potentially the dissemination to regional lymph nodes, spleen, and liver in mice. Shielding of the exposed LPS core through the expression of O-antigen reduces dissemination and infection. Thus, we propose that similar to HIV, S Typhimurium may also utilize APCs via interactions with CD209s as a way to disseminate to the lymph nodes, spleen, and liver to initiate host infection.


Assuntos
Moléculas de Adesão Celular/fisiologia , Lectinas Tipo C/fisiologia , Receptores de Superfície Celular/fisiologia , Salmonella typhimurium/patogenicidade , Animais , Células Apresentadoras de Antígenos/microbiologia , Feminino , Interações Hospedeiro-Patógeno , Humanos , Lipopolissacarídeos/fisiologia , Mananas/farmacologia , Camundongos , Camundongos Endogâmicos C57BL , Antígenos O/fisiologia , Nódulos Linfáticos Agregados/fisiologia , Fagocitose , Células RAW 264.7
9.
Front Immunol ; 10: 96, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30915064

RESUMO

Yersinia pestis, a Gram-negative bacterium and the etiologic agent of plague, has evolved from Yersinia pseudotuberculosis, a cause of a mild enteric disease. However, the molecular and biological mechanisms of how Y. pseudotuberculosis evolved to such a remarkably virulent pathogen, Y. pestis, are not clear. The ability to initiate a rapid bacterial dissemination is a characteristic hallmark of Y. pestis infection. A distinguishing characteristic between the two Yersinia species is that Y. pseudotuberculosis strains possess an O-antigen of lipopolysaccharide (LPS) while Y. pestis has lost the O-antigen during evolution and therefore exposes its core LPS. In this study, we showed that Y. pestis utilizes its core LPS to interact with SIGNR1 (CD209b), a C-type lectin receptor on antigen presenting cells (APCs), leading to bacterial dissemination to lymph nodes, spleen and liver, and the initiation of a systemic infection. We therefore propose that the loss of O-antigen represents a critical step in the evolution of Y. pseudotuberculosis into Y. pestis in terms of hijacking APCs, promoting bacterial dissemination and causing the plague.


Assuntos
Moléculas de Adesão Celular/imunologia , Interações Hospedeiro-Patógeno/imunologia , Lectinas Tipo C/imunologia , Lipopolissacarídeos/imunologia , Peste/imunologia , Receptores de Superfície Celular/imunologia , Yersinia pestis/fisiologia , Animais , Células Apresentadoras de Antígenos/imunologia , Moléculas de Adesão Celular/genética , Linhagem Celular , Feminino , Células HeLa , Humanos , Lectinas Tipo C/genética , Macrófagos/imunologia , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptores de Superfície Celular/genética , Yersinia pseudotuberculosis/fisiologia , Infecções por Yersinia pseudotuberculosis/imunologia
10.
Artigo em Inglês | MEDLINE | ID: mdl-31998663

RESUMO

Uropathogenic Escherichia coli (UPEC), a Gram-negative bacterial pathogen, is a major causative agent of urinary tract infections (UTIs). However, the molecular mechanisms of how UPEC causes infections have not been determined. Recent studies indicated that certain enteric Gram-negative bacteria interact with and hijack innate immune receptors DC-SIGN (CD209a) and SIGNR1 (CD209b), often expressed by antigen-presenting cells (APCs), such as macrophages, leading to dissemination and infection. It was not known whether UPEC could utilize DC-SIGN receptors to promote its infection and dissemination similarly to the enteric pathogens. The results of this study reveal that UPEC interacts with CD209-expressing macrophages and transfectants. This interaction is inhibited by anti-CD209 antibody, indicating that CD209s are receptors for UPEC. Additionally, in contrast to the results of previous studies, mice lacking SIGNR1 are more susceptible to infection of this uropathogen, leading to prolonged bacterial persistence. Overall, the results of our study indicate that the innate immune receptor CD209s participate in the clearance of UPEC during UTIs.


Assuntos
Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/microbiologia , Infecções Urinárias/imunologia , Infecções Urinárias/microbiologia , Escherichia coli Uropatogênica/imunologia , Animais , Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Linhagem Celular , Modelos Animais de Doenças , Infecções por Escherichia coli/patologia , Humanos , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Macrófagos/imunologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fagocitose , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Sistema Urinário/microbiologia , Infecções Urinárias/patologia , Escherichia coli Uropatogênica/patogenicidade
11.
Infect Immun ; 87(1)2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30348825

RESUMO

Yersinia pseudotuberculosis is a Gram-negative enteropathogen and causes gastrointestinal infections. It disseminates from gut to mesenteric lymph nodes (MLNs), spleen, and liver of infected humans and animals. Although the molecular mechanisms for dissemination and infection are unclear, many Gram-negative enteropathogens presumably invade the small intestine via Peyer's patches to initiate dissemination. In this study, we demonstrate that Y. pseudotuberculosis utilizes its lipopolysaccharide (LPS) core to interact with CD209 receptors, leading to invasion of human dendritic cells (DCs) and murine macrophages. These Y. pseudotuberculosis-CD209 interactions result in bacterial dissemination to MLNs, spleens, and livers of both wild-type and Peyer's patch-deficient mice. The blocking of the Y. pseudotuberculosis-CD209 interactions by expression of O-antigen and with oligosaccharides reduces infectivity. Based on the well-documented studies in which HIV-CD209 interaction leads to viral dissemination, we therefore propose an infection route for Y. pseudotuberculosis where this pathogen, after penetrating the intestinal mucosal membrane, hijacks the Y. pseudotuberculosis-CD209 interaction antigen-presenting cells to reach their target destinations, MLNs, spleens, and livers.


Assuntos
Moléculas de Adesão Celular/metabolismo , Células Dendríticas/microbiologia , Endocitose , Interações Hospedeiro-Patógeno , Lectinas Tipo C/metabolismo , Lipopolissacarídeos/metabolismo , Macrófagos/microbiologia , Receptores de Superfície Celular/metabolismo , Yersinia pseudotuberculosis/patogenicidade , Animais , Aderência Bacteriana , Células Cultivadas , Modelos Animais de Doenças , Humanos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Ligação Proteica , Yersiniose/microbiologia , Yersiniose/patologia , Yersiniose/fisiopatologia
12.
Biol Pharm Bull ; 41(8): 1178-1185, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30068867

RESUMO

Cardiorenal syndrome (CRS) is a frequently encountered clinical condition when the dysfunction of either the heart or kidneys amplifies the failure progression of the other organ. CRS remains a major global health problem. Qiliqiangxin (QLQX) is a traditional Chinese herbs medication, which can improve cardiac function, urine volume, and subjective symptoms in patients with chronic heart failure. In the present study, we aim to investigate the role of QLQX in the treatment of CRS type I and the possible mechanism through establishment of a rat model of myocardial infarction. Rats in CRS-Q group were orally treated with QLQX daily for 2 weeks or 4 weeks, while in sham group and CRS-C group were treated with saline at the same time. Enzyme-linked immunosorbent assay (ELISA) analysis showed that QLQX significantly reduced the levels of angiotensin II (AngII), brain natriuretic peptides (BNP), creatinine (CRE), cystatin C (CysC), tumor necrosis factor (TNF)-α, interleukin (IL)-6, microalbuminuria (MAU), and neutrophil gelatinase-associated lipocalin (NGAL) in plasma induced by myocardial infarction. Western blot analysis showed that QLQX significantly reduced the expressions of AngII, non-phagocytic cell oxidase (NOX)2, and B-cell lymphoma (Bcl)2 associated X protein (Bax), and increased the expressions of Bcl2 and Angiotensin II Type 1 receptor (ATR) in the kidney as compared with the CRS-C group. Fluorescence microscopy showed that the content of reactive oxygen species (ROS) was significantly reduced in the kidney as compared with the CRS-C group. We also examined the apoptosis level in kidney by using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, and the result showed that QLQX significantly reduced the apoptosis level in kidney induced by myocardial infarction. Taken together, we suggest that QLQX may be a potentially effective drug for the treatment of CRS by regulating inflammatory/oxidative stress signaling.


Assuntos
Anti-Inflamatórios , Antioxidantes , Síndrome Cardiorrenal/tratamento farmacológico , Medicamentos de Ervas Chinesas , Infarto do Miocárdio/tratamento farmacológico , Albuminúria/sangue , Albuminúria/tratamento farmacológico , Albuminúria/metabolismo , Angiotensina II/sangue , Angiotensina II/metabolismo , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Síndrome Cardiorrenal/sangue , Síndrome Cardiorrenal/metabolismo , Creatinina/sangue , Cistatina C/sangue , Medicamentos de Ervas Chinesas/farmacologia , Medicamentos de Ervas Chinesas/uso terapêutico , Interleucina-6/sangue , Rim/efeitos dos fármacos , Rim/metabolismo , Masculino , Infarto do Miocárdio/sangue , Infarto do Miocárdio/metabolismo , NADPH Oxidase 2/metabolismo , Peptídeo Natriurético Encefálico/sangue , Estresse Oxidativo/efeitos dos fármacos , Fitoterapia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Receptor Tipo 1 de Angiotensina/metabolismo , Transdução de Sinais/efeitos dos fármacos , Fator de Necrose Tumoral alfa/sangue
13.
J Huazhong Univ Sci Technolog Med Sci ; 36(3): 344-349, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27376801

RESUMO

Lipooligosacharide (LOS) of Neisseria gonorrhoeae (gonococci, GC) is involved in the interaction of GC with host cells. Deletion of the alpha-oligosaccharide (alpha-OS) moiety of LOS (lgtF mutant) significantly impairs invasion of GC into epithelial cell lines. GC opacity (Opa) proteins, such as OpaI, mediate phagocytosis and stimulate chemiluminescence responses in neutrophils in part through interaction with members of the carcinoembryonic antigen (CEA) family, which includes CEACAM3 (CD66d), a human neutrophil specific receptor for phagocytosis of bacteria. In the present work, we examined the effects of OpaI-expressing lgtF mutant on phagocytosis by HeLa-CEACAM3 cells and chemiluminescence responses in neutrophils. The results showed that lgtF mutant even expressing OpaI completely lost the ability to promote either phagocytosis mediated by CEACAM3 interaction in HeLa cells or chemiluminescence responses in neutrophils. These data indicated that Opa proteins in the lgtF mutant, which might result from the conformational change, cannot be functional.


Assuntos
Antígenos de Bactérias/química , Antígeno Carcinoembrionário/imunologia , Lipopolissacarídeos/química , Neisseria gonorrhoeae/metabolismo , Neutrófilos/imunologia , Antígenos de Bactérias/genética , Antígenos de Bactérias/imunologia , Antígenos de Bactérias/metabolismo , Sequência de Carboidratos , Antígeno Carcinoembrionário/genética , Regulação da Expressão Gênica , Células HeLa , Interações Hospedeiro-Patógeno , Humanos , Lipopolissacarídeos/imunologia , Medições Luminescentes , Mutação , Neisseria gonorrhoeae/genética , Neisseria gonorrhoeae/patogenicidade , Neutrófilos/microbiologia , Fagocitose
14.
PLoS One ; 10(10): e0141552, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26513146

RESUMO

BACKGROUND: After the implementation of the universal salt iodization (USI) program in 1996, seven cross-sectional school-based surveys have been conducted to monitor iodine deficiency disorders (IDD) among children in eastern China. OBJECTIVES: This study aimed to examine the correlation of total goiter rate (TGR) with average thyroid volume (Tvol) and urinary iodine concentration (UIC) in Jiangsu province after IDD elimination. DESIGN: Probability-proportional-to-size sampling was applied to select 1,200 children aged 8-10 years old in 30 clusters for each survey in 1995, 1997, 1999, 2001, 2002, 2005, 2009 and 2011. We measured Tvol using ultrasonography in 8,314 children and measured UIC (4,767 subjects) and salt iodine (10,184 samples) using methods recommended by the World Health Organization. Tvol was used to calculate TGR based on the reference criteria specified for sex and body surface area (BSA). RESULTS: TGR decreased from 55.2% in 1997 to 1.0% in 2009, and geometric means of Tvol decreased from 3.63 mL to 1.33 mL, along with the UIC increasing from 83 µg/L in 1995 to 407 µg/L in 1999, then decreasing to 243 µg/L in 2005, and then increasing to 345 µg/L in 2011. In the low goiter population (TGR < 3.9%), TGR was positively associated with average Tvol (r = 0.99); UIC showed a non-linear association with average Tvol, and UIC > 300 µg/L was associated with a smaller average Tvol in children. CONCLUSIONS: After IDD elimination in Jiangsu province in 2001, lower TGR was associated with smaller average Tvol. Average Tvol was more sensitive than TGR in detecting the fluctuation of UIC. A UIC of 300 µg/L may be defined as a critical value for population level iodine status monitoring.


Assuntos
Bócio/epidemiologia , Iodo/urina , Glândula Tireoide/patologia , Criança , China , Feminino , Bócio/prevenção & controle , Humanos , Iodo/administração & dosagem , Masculino
15.
Immunol Cell Biol ; 93(9): 815-24, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25829141

RESUMO

Yersinia pestis is a Gram-negative bacterium that causes plague. After Y. pestis overcomes the skin barrier, it encounters antigen-presenting cells (APCs), such as Langerhans and dendritic cells. They transport the bacteria from the skin to the lymph nodes. However, the molecular mechanisms involved in bacterial transmission are unclear. Langerhans cells (LCs) express Langerin (CD207), a calcium-dependent (C-type) lectin. Furthermore, Y. pestis possesses exposed core oligosaccharides. In this study, we show that Y. pestis invades LCs and Langerin-expressing transfectants. However, when the bacterial core oligosaccharides are shielded or truncated, Y. pestis propensity to invade Langerhans and Langerin-expressing cells decreases. Moreover, the interaction of Y. pestis with Langerin-expressing transfectants is inhibited by purified Langerin, a DC-SIGN (DC-specific intercellular adhesion molecule 3 grabbing nonintegrin)-like molecule, an anti-CD207 antibody, purified core oligosaccharides and several oligosaccharides. Furthermore, covering core oligosaccharides reduces the mortality associated with murine infection by adversely affecting the transmission of Y. pestis to lymph nodes. These results demonstrate that direct interaction of core oligosaccharides with Langerin facilitates the invasion of LCs by Y. pestis. Therefore, Langerin-mediated binding of Y. pestis to APCs may promote its dissemination and infection.


Assuntos
Células Apresentadoras de Antígenos/imunologia , Antígenos CD/imunologia , Lectinas Tipo C/imunologia , Lectinas de Ligação a Manose/imunologia , Fagocitose/imunologia , Yersinia pestis/imunologia , Animais , Células Apresentadoras de Antígenos/microbiologia , Antígenos CD/metabolismo , Aderência Bacteriana/imunologia , Células CHO , Células Cultivadas , Cricetinae , Cricetulus , Células Dendríticas/imunologia , Células Dendríticas/metabolismo , Citometria de Fluxo , Interações Hospedeiro-Patógeno/imunologia , Humanos , Células de Langerhans/imunologia , Células de Langerhans/metabolismo , Lectinas Tipo C/metabolismo , Lectinas de Ligação a Manose/metabolismo , Camundongos , Antígenos O/imunologia , Antígenos O/metabolismo , Peste/imunologia , Peste/microbiologia , Ligação Proteica/imunologia , Receptores de Superfície Celular/imunologia , Receptores de Superfície Celular/metabolismo , Análise de Sobrevida , Yersinia pestis/metabolismo , Yersinia pestis/fisiologia
16.
J Cosmet Sci ; 62(2): 127-37, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21635842

RESUMO

A new test method has been developed to determine surface energy of hair fibers through measurements of contact angles at two hair/liquid interfaces. By measuring changes in surface energy of the same hair fiber before and after a cosmetic treatment, effects of active ingredients and the performance of tested formulations can be evaluated.The establishment of the method is based on Fowkes theory (1,2) described with two components, a dispersive and a non-dispersive component. The non-polar liquid used in this study was diiodomethane, and the polar liquid was benzyl alcohol. A Kruss 100 Tensiometer was used to measure contact angles of hair fibers. Virgin dark brown and regular bleached hairs were treated with selected conditioner formulations. Reductions in combing forces of hair tresses before and after respective treatments were correlated with decreases in average surface energy of hair fibers obtained from the corresponding tresses.Experimental results indicate that the average surface energy of hair fibers treated with conditioners decreases and the hydrophobicity of the hair surface increases, the results correlate well with the reduction in combing forces after respective treatments. This research work provides a new methodology to evaluate/screen conditioning performance of hair care ingredients and formulations for development of better products.


Assuntos
Preparações para Cabelo , Cabelo/fisiologia , Humanos , Propriedades de Superfície
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