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1.
Artigo em Inglês | MEDLINE | ID: mdl-32682876

RESUMO

Cognitive behavioral therapy (CBT) is considered a first-line treatment for patients with obsessive-compulsive disorder (OCD), and it possesses advantages over pharmacological treatments in stronger tolerance to distress, lower rates of drop out and relapse, and no physical "side-effects". Previous studies have reported CBT-related alterations in focal brain regions and connections. However, the effects of CBT on whole-brain structural networks have not yet been elucidated. Here, we collected diffusion MRI data from 34 unmedicated OCD patients before and after 12 weeks of CBT. Fifty healthy controls (HCs) were also scanned twice at matched intervals. We constructed individual brain white matter connectome and performed a graph-theoretical network analysis to investigate the effects of CBT on whole-brain structural topology. We observed significant group-by-time interactions on the global network clustering coefficient and the nodal clustering of the left lingual gyrus, the left middle temporal gyrus, the left precuneus, and the left fusiform gyrus of 26 CBT responders in OCD patients. Further analysis revealed that these CBT responders showed prominently higher global and nodal clustering compared to HCs at baseline and reduced to normal levels after CBT. Such significant changes in the nodal clustering of the left lingual gyrus were also found in 8 CBT non-responders. The pre-to-post decreases in nodal clustering of the left lingual gyrus and the left fusiform gyrus positively correlated with the improvements in obsessive-compulsive symptoms in the CBT-responding patients. These findings indicated that the network segregation of the whole-brain white matter network in OCD patients was abnormally higher and might recover to normal after CBT, which provides mechanistic insights into the CBT response in OCD and potential imaging biomarkers for clinical practice.

2.
Food Chem ; 338: 127886, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32829294

RESUMO

Laser-induced breakdown spectroscopy (LIBS) was used to rapidly detect heavy metals in mulberry leaves. For the purpose of increasing detection stability and accuracy, a novel analysis framework consisting of a Kohonen self-organizing map (SOM), a variable selection method using the successive projection algorithm (SPA) and uninformative variable elimination (UVE), and a consensus modeling strategy was proposed for processing LIBS data to determine copper (Cu) and chromium (Cr) content. Results showed that the best regression model for Cu and Cr content achieved the residual predictive deviation (RPD) values of 10.0494 and 8.3874, respectively, and root mean square error of prediction (RMSEP) values of 110.4550 and 41.4561, respectively. The proposed strategy provides a high-accuracy and rapid alternative to the traditional method for monitoring heavy metals in mulberry leaves, which could guarantee the quality of mulberry leaves and potentially be used in food-related industries.

3.
Food Chem ; 334: 127474, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32688175

RESUMO

Rapid and accurate detection of pesticide residues in food matrices are of great significance to food safety. This study aimed to characterize the fingerprint peaks of 2,4-dichlorophenoxyacetic acid (2,4-D) and to enhance its detection accuracy in food matrices by using terahertz (THz) time-domain spectroscopy. Density functional theory was used to simulate molecular dynamics of 2,4-D peaks (1.35, 1.60, 2.37 and 3.00 THz). Four baseline correction methods, including asymmetric least squares smoothing (AsLS), adaptive iteratively reweighted penalized least squares (AirPLS), background correction (Backcor), baseline estimation and denoising with sparsity (BEADS) were compared and used to eliminate spectral baselines of Zizania latifolia (ZIZLA), rice and maize containing 2,4-D residues, from 0.1 to 4 THz. Based on the peak information of 1.35 THz, the detection limit and accuracy of 2,4-D residues in these food matrices were significantly improved after THz spectral baseline correction, providing a new feasibility for food safety and agricultural applications.

4.
Liver Transpl ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33035403

RESUMO

We read with great interests on the paper by Croome et al on phenomenon of reversal on macrosteatosis (MaS) for grafts after implantation in vivo for 7 days [1]. According to the sequential biopsies, the authors revealled most MaS grafts were reversed to absence of steatosis in early phase after LT. And this status was maintained in long-term follow up duration (six months). The authors didn't report the differentiated impact of MaS/non-MaS on post-transplant outcomes, although the existence of MaS was still remained in some samples (three in one week and five in six months after LT).

5.
Artigo em Inglês | MEDLINE | ID: mdl-33027009

RESUMO

This article investigates the stability of the switched neural networks (SNNs) with a time-varying delay. To effectively guarantee the stability of the considered system with unstable subsystems and reduce conservatism of the stability criteria, admissible edge-dependent average dwell time (AED-ADT) is first utilized to restrict switching signals for the continuous-time SNNs, and multiple Lyapunov-Kravosikii functionals (LKFs) combining relaxed integral inequalities are employed to develop two novel less-conservative stability conditions. Finally, the numeral examples clearly indicate that the proposed criteria can reduce conservatism and ensure the stability of continuous-time SNNs.

6.
Chem Commun (Camb) ; 2020 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-33030154

RESUMO

Herein, a new fluorescent probe, AE-Phos, is reported for detecting the ALP activity with the combined advantages of aggregation-induced emission (AIE) and excited state intramolecular proton transfer (ESIPT). Further detailed fluorescence experiments demonstrated that AE-Phos exhibited excellent selectivity and sensitivity, a large Stokes shift, and a fast response towards ALP. Furthermore, AE-Phos was applied to imaging the ALP activity in different cell lines quantitatively.

7.
Dalton Trans ; 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33057519

RESUMO

In this work, different synthetic methods were used to prepare metal borates. One cadmium borate Cd3[B6O9(OH)2]2·2NO3·4H2O (1) and two nickel borate Ni0.8Mn0.2{B6O7(OH)3[O(CH2)2NH2]3} (2), [Ni(1-MI)6]·[B5O6(OH)4]2·2DMF (3, 1-MI = 1-methylimidazole, DMF = N,N-dimethylformamide) species were successfully prepared. The structures of these three compounds were confirmed via single crystal powder X-ray diffraction (SCPXRD), elemental analysis, FTIR, PXRD, and ICP studies. Compound 1 was prepared via molten salt synthesis, and compounds 2 and 3 were prepared via precursor synthesis, which is a synthetic method that uses a certain polyanionic cluster as a reagent. Compound 1 shows a novel three-dimensional (3D) structure, in which the [B6O11(OH)2] units acting as the basic polyanionic cluster form a two-dimensional (2D) anionic structure. In addition, the unexpected Cd3O14 clusters are connected with the 2D structures, leading to the 3D structure. The crystal structure of 2 is packed with very large neutral Ni0.8Mn0.2{B6O7(OH)3[O(CH2)2NH2]3} oxo-metal-boron clusters. The crystal structure of 3 consists of a [B5O6(OH)4]- cluster, a DMF molecule, and a [Ni(1-MI)6]2+ complex. In particular, 2 was successfully constructed using the same hexaborate cluster as its precursor. However, 3 only contains Ni2+ cations when a neutral NixMn1-x[B6O7(OH)6] cluster was used as an initial reagent, and it shows an ability to select a certain metal cation during the self-assembly process. Moreover, compounds 1, 2, and 3 exhibit different wide band gaps of 3.06 eV (1), 3.95 eV (2), and 4.24 eV (3).

8.
J Hazard Mater ; 404(Pt B): 124180, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33059152

RESUMO

Microplastics pollution in river systems has generated great concern; however, few studies have focused on the contributions of multiple influencing factors to microplastics in river systems. In the current study, we utilized data on microplastics in surface sediments from the Haihe River, a seagoing river in northern China to establish a generalized additive model (GAM) for quantifying the effects of multiple factors on the distribution of microplastics. A high abundance of microplastics (4980 ± 2462 items∙kg-1 dry weight) was found. Small particles (< 1000 µm) accounted for a dominant proportion (44.8-61.0%). Polyethylene (PE) was the chief component with an averaged fraction of 49.3%, in which low- and high-density polyethylene contributed 90.7% and 9.3% of the PE, respectively. Microplastics abundance was positively correlated with sediment TOC and the silt fraction (p < 0.05) but negatively correlated with the sand fraction (p < 0.05). The GAM could explain approximate 60% of the total microplastics abundance, and dam (28.5%), sediment TOC (22.9%), and sewage effluent (17.6%) were the main contributors to total variations in microplastics abundance. Local sewage effluent acted as an important point source of microplastics discharge, and the dam on the river greatly affected the deposition and accumulation of microplastics.

9.
J Vasc Surg ; 2020 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-33091518

RESUMO

OBJECTIVE: An arteriovenous fistula (AVF) is the preferred vascular access for chronic hemodialysis, yet the rates of AVF maturation failure and reintervention remain high. We investigated the AVF geometric parameters and their associations with AVF physiologic maturation and reintervention in a prospective multi-center study. METHODS: From 2011 to 2016, patients undergoing a vein end-to-artery side upper-extremity AVF creation surgery were recruited. Contrast-free dark-blood and phase-contrast magnetic resonance imaging (MRI) scans were performed using 3.0T scanners to obtain AVF lumen geometry and flow rates, respectively, at postoperative Day 1, Week 6, and Month 6. Arterial-venous anastomosis angle, nonplanarity, and tortuosity of the fistula were calculated based on lumen centerlines. AVFs were considered physiologically matured if, based on the Week 6 MRI data, the flow rate was at least 500 mL/min, and the minimum vein lumen diameter was at least 5 mm. Associations of these geometric parameters with AVF maturation and reintervention due to peri-anastomotic and mid-vein stenosis within one year were assessed. RESULTS: There were 111 patients with usable Day 1 scan, with the majority being upper-arm AVFs (n = 73). Compared to forearm AVFs, upper-arm AVFs had greater anastomosis angles (P < .001), larger deviations from a plane (nonplanarity, P = .002), and more prominent tortuosity (P = .038) at Day 1. These parameters significantly increased between Day 1 and Week 6 in upper-arm AVFs; in contrast, significant changes in these parameters in forearm AVFs were not observed. The rate of maturation was 54% and 86% for forearm and upper-arm AVFs, respectively, with none of the geometric parameters at Day 1 being associated with maturation for AVFs in either location. The rate of reintervention was 24% and 30% for forearm and upper-arm AVFs, respectively, with a larger nonplanarity angle at Day 1 being associated with less reintervention (30° ± 15° vs. 21° ± 10°, P = .034) in upper-arm AVFs only. This relationship was unchanged after adjusting for age, sex, race, dialysis status, or diabetes. CONCLUSIONS: Upper-arm fistulas have a larger anastomosis angle, are more nonplanar, and have more tortuous veins than forearm fistulas. For upper-arm fistulas, a larger nonplanarity angle is associated with a lower rate of reintervention within one year. Once confirmed, vascular surgeons may consider increasing the nonplanarity angle by incorporating a tension-free gentle curvature in the proximal segment of the mobilized vein to reduce reinterventions when creating an upper-arm fistula.

10.
Small ; : e2003797, 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33103353

RESUMO

The integration of microfluidics with biomedical research is confronted with considerable limitations due to its body materials. With high content of water, hydrogels own superior biocompatibility and degradability. Can hydrogels become another material choice for the construction of microfluidic chips, particularly biofluidics? The present review aims to systematically establish the concept of hydrogel-based microfluidic chips (HMCs) and address three main concerns: i) why choosing hydrogels? ii) how to fabricate HMCs?, and iii) in which fields to apply HMCs? It is envisioned that hydrogels may be used increasingly as substitute for traditional materials and gradually act as the body material for microfluidic chips. The modifications of conventional process are highlighted to overcome issues arising from the incompatibility between the construction methods and hydrogel materials. Specifically targeting at the "soft and wet" hydrogels, an efficient flowchart of "i) high resolution template printing; ii) damage-free demolding; iii) twice-crosslinking bonding" is proposed. Accordingly, a broader microfluidic chip concept is proposed in terms of form and function. Potential biomedical applications of HMCs are discussed. This review also highlights the challenges arising from the material replacement, as well as the future directions of the proposed concept. Finally, the authors' viewpoints and perspectives for this emerging field are discussed.

11.
Sci Total Environ ; : 142750, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33077213

RESUMO

Bauxite residue has poor physical conditions, which hinders plant growth and causes potential environmental risks. Polymer materials have broad potential applications for holding water and improving soil aggregation. However, no attempt has been made to assess the effects of polymers on physical structure of bauxite residue. The purpose of this study was to evaluate the effects of polyacrylamide (BP), humic acid (BH), starch-acrylamide polymer (BSA) and humic acid-acrylamide polymer (BHA) on aggregate formation, stability, and pore characteristics in bauxite residue by 60-day pot experiment. Results demonstrated that 0.2% polymer addition increased the proportion of >0.25 mm mechanical-stable aggregates. Under wet sieving, BP and BHA treatments increased the values of mean weight diameter (WMWD) from 0.36 mm to 0.67 mm and 0.68 mm, respectively, which may result in the increase of the percentage of organic functional groups including OCO and CC. Laser diffraction analysis and the visualized 3D surface map revealed that >0.25 mm residue aggregate was more difficult to disintegrate following BHA treatment during 180-min hydraulic circulation. BP and BH treatments elevated <0.5 µm pore size volumes, whilst BHA treatment increased >5 µm pore size volumes and improved the porosity of bauxite residue. Polymer applications indicated that compared with polyacrylamide or humic acid, humic acid-acrylamide polymer could be regarded as an effective ameliorant due to its positive effects on both aggregate stability and pore characteristics. These findings were helpful for understanding the application potential of natural-synthetic polymers on physical conditions of bauxite residue prior to ecological reconstruction on the disposal areas.

12.
Technol Cancer Res Treat ; 19: 1533033820957000, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33000682

RESUMO

microRNA-663a (miR-663a) was reported to be highly expressed in cancers. However, its roles in melanoma progression remain unclear. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was conducted to measure miR-663a expression level in melanoma cell lines and normal cells. Cell counting kit-8 assay, wound-healing assay, and transwell invasion assay were conducted to analyze biological roles of miR-663a in melanoma. Luciferase activity reporter assay was conducted to validate the connection of miR-663a and Four and a half LIM domain (FHL) protein 3 (FHL3) in melanoma. Our results showed miR-663a expression level was significantly increased in melanoma cells compared with normal cells. Silencing miR-663a expression suppresses melanoma cell proliferation, migration, and invasion in vitro. Moreover, FHL3 was validated as a functional target of miR-663a. Knockdown of FHL3 partially rescued the inhibitory effects of miR-663a inhibitor on melanoma cell behaviors. Together, our work provided evidence that miR-663a functions as an oncogenic miRNA in melanoma.

13.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 32(9): 1131-1134, 2020 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-33081905

RESUMO

According to the world epidemic report, the mortality of patients with severe coronavirus disease 2019 (COVID-19) is high. Diabetic patients are more susceptible to COVID-19. Since the mortality of COVID-19 patients with diabetes is on the top of list, hyperglycemia is considered an independent risk factor for severe COVID-19. Up to now, there is few effective treatment for severe patients infected with 2019 novel coronavirus (2019-nCoV). Clinical studies observed that cytokine storms existed in patients with severe COVID-19. Sustained high levels of cytokines cause diffuse damage to pulmonary capillary endothelial cells and alveolar epithelial cells, resulting in acute respiratory distress syndrome (ARDS). ARDS is the main cause of death in COVID-19 patients. Host-directed therapy (HDT) is an emerging therapeutic method in the field of anti-infection, which can activate the self-protective immune response, suppress excessive inflammatory response, and be used to assist the treatment of traditional drugs to shorten the course of disease. Metformin has been shown to be effective in HDT and can assist in the treatment of the viral and bacterial infectious disease. This paper discusses the rationality and potential therapeutic mechanism of metformin in the treatment of severe COVID-19. It was speculated that the use of metformin for controlling blood glucose in severe COVID-19 patients with diabetes may prevent or inhibit the occurrence of ARDS, thereby reducing the mortality of COVID-19 patients. The possible mechanism is that metformin could inhibit cytokine storm via suppressing interleukin-6 (IL-6) signaling, prevent the process of lung fibrosis, suppress endocytosis, thereby elevating angiotensin converting enzyme 2 (ACE2) expression.


Assuntos
Betacoronavirus , Infecções por Coronavirus , Metformina/uso terapêutico , Pandemias , Pneumonia Viral , Infecções por Coronavirus/tratamento farmacológico , Células Endoteliais , Pneumonia Viral/tratamento farmacológico
14.
Biofabrication ; 2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33105114

RESUMO

Nucleus pulposus (NP) degeneration is the major cause of degenerative disc disease (DDD). This condition cannot be treated or attuned by traditional open or minimally invasive surgical options. However, a combination of stem cells, growth factors (GFs) and biomaterials present a viable option for NP regeneration. Injectable biomaterials act as carriers for controlled release of GFs and deliver stem cells to target tissues through a minimally invasive approach. In this study, injectable Gelatin methacryloyl (GelMA) microspheres (GMs) with controllable, uniform particle sizes were rapidly biosythesized through a low-cost electrospraying method. The GMs were used as delivery vehicles for cells and GFs and they exhibited good mechanical properties, biocompatibilities and enhanced the in vitro differentiation of laden cells into NP-like phenotypes. Furthermore, this integrated system attenuated the in vivo degeneration of rat intervertebral disc, maintained NP tissue integrity and accelerated the synthesis of extracellular matrix (ECM). Therefore, this novel therapeutic system is a promising option for the treatment of Degenerative disc disease (DDD).

15.
Plant Dis ; 2020 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-32910726

RESUMO

Archidendron clypearia (Jack) Benth. (common name: Monkey-pod) is a perennial arbor tree belonging to the family Fabaceae. It is used to produce a traditional medicine to treat a variety of heat toxicity symptoms in China. The distribution of A. clypearia mainly include Guangdong, Fujian, and Zhejiang provinces in China as well as other countries in Southeast Asia. In 2018, about 30 Monkey-pod trees were observed showing typical dieback symptoms in a commercial plantation (114°36'09.401″E, 22°58'38.553″N) of Huizhou, Guangdong Province, China. In addition, white cylindrical sawdust exudates from exit holes of ambrosia beetles were observed on the trunks and main branches of the diseased trees. Discoloration was seen around the exit holes of the beetles by splitting the wood. Samples of symptomatic and asymptomatic wood tissues of branches, trunks, and roots were collected from 11 trees. To identify the pathogen, the samples were disinfected with a sodium hypochlorite solution containing 50 g/l chlorine for 60 s, rinsed three times with sterile distilled water, and placed on potato dextrose agar (PDA) for culture at 25℃ for 14 days. Beetles from exit holes of the pathogen-infected wood tissues were also collected and identified as Xyleborus affinis by their cytochrome c oxidase I (COI) sequences (GenBank accession numbers: MT921005 and MT921006) as well as morphological features. After two weeks incubation, 37 single-spore isolates were obtained (31 from galleries of branches and trunks, and 6 from mycangia and the body surface of beetles). Colonies on PDA were fusarium-like, pale orange, floccose with abundant aerial mycelia, and growing at the rate of 3.0 to 4.5 mm/d at 25℃. Conidiophores from the aerial mycelia often had single phialides. Microconidia were hyaline, 0-1 septate, (4-)6-12(-16) × 2-4 µm (n=100), Macroconidia were hyaline, ellipsoidal to falcate, 2-4 septate, (10-)25-45(-50) × 3-6 µm (n=100), with basal foot cells shaped to pointed and apical cells tapered and curved. Two representative isolates (CYML 367, 368) were used for molecular identification and pathogenicity tests. The internal transcriptional spacer region (ITS), ß-tubulin (TUB), and translation elongation factor-1α (TEF 1-α) regions were amplified with primers ITS1F/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F (Carbone and Kohn 1999) /EF-2 (O'Donnell et al.1998), respectively. DNA extraction and PCR conditions were followed the methods described by Yin et al. (2019). The amplified fragments were sequenced. The results of BLAST demonstrated that the fragment sequences of ITS (468 bp), TEF 1-α (616 bp), and TUB (295 bp) of isolates CYML367 and CYML368 had 100% similarity to correspondence sequences from the Fusarium oxysporum Schlecht. emend. Snyder & Hansen species complex (GenBank accession numbers MT032694, JF740777, and MN451171, respectively). Sequences generated from this study were deposited into GenBank under accession numbers MN826824 and MN826825 (ITS), MN839680 and MN839681 (TUB), and MN839682 and MN839683 (TEF 1-α). Pathogenicity tests were conducted on 2-year-old seedlings (60-70 cm height, 1.0-1.2 cm in diameter) of A. clypearia with the representative isolates. Ten seedlings were inoculated with each isolate. The stem surface was disinfected with 75% ethanol for 30 s, and rinsed with sterilized water, wounded by removing part of the phloem and xylem, and placed with a mycelial plug (5 mm diameter) from the margin of a 7-day-old PDA plate with mycelia facing the cambium. The inoculated wounds were wrapped with a medical sterile gauze to prevent desiccation and contamination. Control plants were inoculated with non-colonized PDA plugs. These inoculated and control plants were incubated in a chamber at 25℃, 50% humidity, 12 h light/12 h dark cycle. After 3 weeks, all inoculated plants displayed Fusarium dieback symptoms similar to those observed on the original diseased trees in the plantation. The average lesion lengths (1.2/1.7 cm) caused by two isolates were all significantly longer than the wounds in the negative controls (P<0.05). The same fungus was re-isolated from the symptomatic plants, thus fulfilling the Koch's postulates. Further studies should be conducted on the potential vector association, transmission route, and management strategies of this pathogen on A. clypearia in growing regions of China. To our best knowledge, this is the first report of F. oxysporum causing dieback on A. clypearia in China.

16.
Artigo em Inglês | MEDLINE | ID: mdl-32924759

RESUMO

A wide variety of leguminous plant-released (iso)flavonoids, such as genistein, are potential inducers of the nodulation (nod) genes of endosymbiotic rhizobia for the production of nodulation factors (Nod factors), which are vital signaling molecules for triggering the symbiotic process. However, these (iso)flavonoids are generally thought to be toxic to the bacterial partner to varying degrees. Here, a novel TetR-like regulator gene of the soybean symbiont Bradyrhizobium diazoefficiens USDA110, bdtR (systematic designation blr7023), was characterized. It was found to be rapidly and preferentially induced by genistein, and its mutation resulted in significantly increased expression of the neighboring bll7019-bll7021 genes, encoding a multidrug resistance efflux pump system, in the absence of this isoflavonoid. Then, the transcriptional start site of BdtR was determined, and it was revealed that BdtR acted as a transcriptional repressor of the above efflux system through the binding of an AT-rich operator, which could be completely prevented by genistein. In addition, the ∆bdtR deletion mutant strain showed higher accumulation of extracellular genistein and became less susceptible to the isoflavonoid. In contrast, the inactivation of BdtR led to the significantly decreased induction of a nodulation gene (nodY) independent of the expression of nodD1 and nodW and to much weaker nodulation competitiveness. Taken together, the results show that BdtR plays an early sensing role in maintaining the intracellular homeostasis of genistein, helping to alleviate its toxic effect on this bacterium by negatively regulating neighboring genes encoding an efflux pump system while being essentially required for nodule occupancy competitiveness.

17.
Sensors (Basel) ; 20(17)2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-32882807

RESUMO

Radix Astragali is a prized traditional Chinese functional food that is used for both medicine and food purposes, with various benefits such as immunomodulation, anti-tumor, and anti-oxidation. The geographical origin of Radix Astragali has a significant impact on its quality attributes. Determining the geographical origins of Radix Astragali is essential for quality evaluation. Hyperspectral imaging covering the visible/short-wave near-infrared range (Vis-NIR, 380-1030 nm) and near-infrared range (NIR, 874-1734 nm) were applied to identify Radix Astragali from five different geographical origins. Principal component analysis (PCA) was utilized to form score images to achieve preliminary qualitative identification. PCA and convolutional neural network (CNN) were used for feature extraction. Measurement-level fusion and feature-level fusion were performed on the original spectra at different spectral ranges and the corresponding features. Support vector machine (SVM), logistic regression (LR), and CNN models based on full wavelengths, extracted features, and fusion datasets were established with excellent results; all the models obtained an accuracy of over 98% for different datasets. The results illustrate that hyperspectral imaging combined with CNN and fusion strategy could be an effective method for origin identification of Radix Astragali.

18.
Med Sci Monit ; 26: e924884, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32964894

RESUMO

BACKGROUND The aim of this study was to review outcomes of gamma knife radiosurgery (GKRS) for prolactinoma and report our experience with it. MATERIAL AND METHODS We reviewed the patient database in our center and identified 24 patients with prolactinoma who underwent GKRS from 1993 to 2016.  Complete endocrine, clinical, and radiological data were available on these individuals before and after GKRS. RESULTS Data from 5 males and 19 females with a median age of 30.5 years (range, 18.1 to 51.1) were reviewed. The median follow-up was 109.3 months (range, 23.2-269.3). The median margin dose of GKRS was 15 Gy (range, 10.5 to 23.6). In total, prolactin (PRL) normalization after GKRS was achieved in 66.7% of patients. Endocrine remission (normal PRL levels after discontinuation of dopamine agonists) was achieved in 10 patients (41.7%), and endocrine control (normal PRL levels while taking dopamine agonists) was achieved in 6 patients (25.0%). All of the patients showed tumor control. New-onset hypopituitarism post-GKRS occurred in 4 patients (16.7%). No new visual dysfunction or cranial nerve dysfunction were observed after GKRS. CONCLUSIONS For treatment of prolactinomas, GKRS may provide relatively high rates of endocrine remission and tumor control, as well as a low rate of new-onset hypopituitarism. GKRS may be an effective and safe treatment for prolactinomas.

19.
Lung Cancer ; 149: 33-40, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32956986

RESUMO

BACKGROUND: Osimertinib is the treatment of choice for advanced EGFR-mutant non-small cell lung cancer (NSCLC). However, novel strategies to improve the duration of disease control are still urgently needed. Aspirin has been shown to decrease cancer incidence and improve outcomes in various malignancies. Therefore, we evaluated a cohort of patients who received osimertinib with or without concurrent use of aspirin to assess whether the addition of aspirin may lead to improved clinical outcomes. METHODS: MD Anderson Cancer Center GEMINI database was retrospectively queried for EGFR-mutant NSCLC patients who received osimertinib with or without concurrent use of aspirin for progression-free survival (PFS) and overall survival (OS). RESULTS: A total of 365 patients were identified including 77 which had concurrent use of aspirin. Patients in the aspirin-osimertinib group had significantly improved PFS (21.3 vs 11.6 months; HR, 0.52; 95 % CI, 0.38-0.70) and OS (Not reached vs 32.3 months; HR, 0.56; 95 % CI, 0.35-0.91) compared to osimertinib group. In subgroup analyses, the aspirin-associated PFS benefit was observed in patients with and without central nervous system (CNS) metastases, as well as in osimertinib first-line setting and in subsequent line setting. The median PFS in EGFR 19Del patients was longer than EGFR L858R patients with osimertinib, and when aspirin was added, the median PFS significantly improved in both groups regardless of lines of therapy. The benefit from aspirin was independent of age, gender, TP53 mutational status, or PD-L1 positivity. CONCLUSION: Concurrent aspirin use with osimertinib in EGFR-mutant NSCLC patients was associated with improved survival, regardless of lines of therapy, CNS metastatic status, EGFR mutation type, age, gender, TP53, and PD-L1 status.

20.
J Physiol Sci ; 70(1): 45, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32967614

RESUMO

Diabetic peripheral neuropathy (DPN) is a chronic microvascular complication of diabetes. The purpose of this study is to find the underlying mechanism for the effects of acupuncture in DPN rats. Rats were rendered diabetic with a single injection of 35 mg/kg streptozotocin (STZ). These STZ-diabetic rats were treated with acupuncture for 20 min once daily. The therapeutic efficacy of acupuncture was assessed using mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) evaluations. After 14 days treatment, acupuncture markedly reduced the pathological injury in STZ-diabetic rats. Moreover, it significantly down-regulated P2X4 and OX42 expression along with the reduced levels of inflammatory factors (CXCR3, TNF-α, IL-1ß, IL-6), GSP and lipid metabolisms in the spinal cord of the DPN rats. Acupuncture could relieve DPN in rats by regulating P2X4 expression and inflammation in spinal microglia.

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