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1.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121838, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36108407

RESUMO

Chicken is at risk of contaminated foodborne pathogens in the production process. Timely and nondestructive detection of foodborne pathogens in chicken is essential for food security. The study aims to explore the feasibility of developing efficient classification models for qualitative detection of Staphylococcus aureus in chicken breast using the hyperspectral imaging technique. Principal component analysis was used to process the full spectral information and three wavelength selection methods (competitive adaptive reweighted sampling, genetic algorithm, and successive projections algorithm) were applied to extract effective wavelengths. These methods were combined with the support vector machine algorithm to develop conventional classification models, respectively. In addition, a convolutional neural network model based on deep learning was designed and trained for comparison. The performance of the convolutional neural network model was significantly better than that of conventional classification models. The overall accuracy for chicken sample classifications was improved from 83.88% to 91.38%. The results demonstrated that deep learning can effectively extract spectral features and promote the application of hyperspectral imaging in foodborne pathogens detection of chicken products.


Assuntos
Imageamento Hiperespectral , Staphylococcus aureus , Animais , Galinhas , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Máquina de Vetores de Suporte
2.
Spectrochim Acta A Mol Biomol Spectrosc ; 284: 121785, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36058172

RESUMO

Eating repeatedly used hotpot oil will cause serious harm to human health. In order to realize rapid non-destructive testing of hotpot oil quality, a modeling experiment method of fluorescence hyperspectral technology combined with machine learning algorithm was proposed. Five preprocessing algorithms were used to preprocess the original spectral data, which realized data denoising and reduces the influence of baseline drift and tilt. The feature bands extracted from the spectral data showed that the best feature bands for the two-classification model and the six-classification model were concentrated between 469 and 962 nm and 534-809 nm, respectively. Using the PCA algorithm to visualize the spectral data, the results showed the distribution of the six types of samples intuitively, and indicated that the data could be classified. Based on the modeling analysis of the feature bands, the results showed that the best two-classification models and the best six-classification models were MF-RF-RF and MF-XGBoost-LGB models, respectively, and the classification accuracy reached 100 %. Compared with the traditional model, the error was greatly reduced, and the calculation time was also saved. This study confirmed that fluorescence hyperspectral technology combined with machine learning algorithm could effectively realize the detection of reused hotpot oil.


Assuntos
Algoritmos , Máquina de Vetores de Suporte , Fluorescência , Humanos , Aprendizado de Máquina , Tecnologia
3.
Curr Mol Med ; 2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36330641

RESUMO

BACKGROUND: Our preliminary sequencing analysis revealed increased expression levels of circNUP98 in nasopharyngeal carcinoma (NPC). This study was therefore carried out to explore the role of circNUP98 in NPC. METHODS: The present study enrolled 56 patients with NPC, 44 patients with cervical lymphadenitis (CL), 50 patients with nose bleeding (NB), 50 patients with chronic sinusitis (CS), 50 patients with lymph node tuberculosis (LNT), and 50 healthy controls (Control). Plasma samples were obtained from all patients and the controls. In addition, NPC and paired non-tumor tissue samples were collected from the 56 NPC patients. Expression of circNUP98 in both tissue and plasma samples was determined by RT-qPCR. The 56 NPC patients were followed up for 5 years to analyze the associations between plasma expression of circNUP98 and the survival of patients. The diagnostic value of circNUP98 for NPC was analyzed through ROC curve analysis. RESULTS: The plasma expression levels of circNUP98 were only increased in NPC, but not in CL, NB, CS and LNT groups compared to that in the Control group. In addition, increased expression levels of circNUP98 was observed in NPC tissues compared to that in non-tumor tissues. Plasma circNUP98 was closely correlated with circNUP98 in NPC tissues, but not circNUP98 in non-tumor tissues. With plasma circNUP98 as a biomarker, NPC patients were separated from CL, NB, CS, LNT and the Control groups. Plasma expression of circNUP98 was found to be positively correlated with the poor survival of patients. Moreover, plasma circNUP98 was only closely associated with tumor metastasis, but not tumor size. CONCLUSION: The expression of circNUP98 in plasma may be used to diagnose NPC and predict patients' survival.

4.
Cereb Cortex ; 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36320154

RESUMO

The default mode network (DMN) is a workspace for convergence of internal and external information. The frontal parietal network (FPN) is indispensable to executive functioning. Yet, how they interplay to support cognitive development remains elusive. Using longitudinal developmental fMRI with an n-back paradigm, we show a heterogeneity of maturational changes in multivoxel activity and network connectivity among DMN and FPN nodes in 528 children and 103 young adults. Compared with adults, children exhibited prominent longitudinal improvement but still inferior behavioral performance, which paired with less pronounced DMN deactivation and weaker FPN activation in children, but stronger DMN coupling with FPN regions. Children's DMN reached an adult-like level earlier than FPN at both multivoxel activity pattern and intranetwork connectivity levels. Intrinsic DMN-FPN internetwork coupling in children mediated the relationship between age and working memory-related functional coupling of these networks, with posterior cingulate cortex (PCC)-dorsolateral prefrontal cortex (DLPFC) coupling emerging as most prominent pathway. Coupling of PCC-DLPFC may further work together with task-invoked activity in PCC to account for longitudinal improvement in behavioral performance in children. Our findings suggest that the DMN provides a scaffolding effect in support of an immature FPN that is critical for the development of executive functions in children.

5.
Dalton Trans ; 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377639

RESUMO

Mixed-valence (MV) complexes containing non-innocent ligands are excellent models for the investigation of the electron-transfer process. A series of twelve bimetallic cyanide-bridged complexes [CpMen(dppe)RuCNFeLx][A] (A = PF6- or I-, CpMen = alkyl cyclopentadienyl, dppe = 1,2-bis (diphenylphosphino)ethane, and LX = pentane-2,4-dione-bis (S-alkylisothiosemi-carbazonato); n = 0, x = Methyl (Me), Ethyl (Et), n-Propyl (Pr) and n-Butyl (Bu), and A = PF6-, 1Me[PF6], 1Et[PF6], 1Pr[PF6], and 1Bu[PF6]; n = 1, x = Me, Et, Pr, and Bu, and A = PF6-, 2Me[PF6], 2Et[PF6], 2Pr[PF6], and 2Bu[PF6]; n = 5, x = Me, Et, Pr, and Bu, and A = I-, 3Me[I], 3Et[I], 3Pr[I], and 3Bu[I]) have been synthesized and well characterized. The investigations demonstrate that all the cations of the complexes could be described with the basic electronic configuration , in which the fragment could be regarded as being delocalized. The ligand to metal charge transfer (LMCT) transition in the fragment and the low-spin RuII to the intermediate-spin FeIII charge transfer (MMCT) transition have been investigated. The UV-vis-NIR spectral analysis results suggest that the energy of the LMCT transition is lower than that of the MMCT transition due to electron delocalization between the non-innocent ligand and the FeIII ion, which is strongly supported by TDDFT calculations. Furthermore, the RuII → FeIII MMCT energy decreases and the LMCT energy increases with the increasing electron donating ability of the ancillary ligands from Cp, CpMe to CpMe5, but slightly changes with the variation of the ligand Lx from Me, Et, Pr to Bu. Compared to the MMCT energy change, however, the energy of the LMCT from to FeIII in the delocalized moiety is less influenced by the electronic effect of the ancillary ligand or the CpMen(dppe)RuIICN (n = 0, 1 and 5) fragment.

6.
Lab Chip ; 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36378140

RESUMO

Rice false smut spores (RFSS), which are airborne spores caused by Ustilaginoidea virens (U. virens), not only cause severe yield loss and grain quality reduction, but also produce toxins that are harmful to humans and animals. Nucleic acid detection has become the main method for RFSS monitoring due to its high specificity and sensitivity. However, nucleic acid detection requires multiple steps of spore collection, DNA extraction, nucleic acid amplification and detection, which has a high demand for personnel and is hard to link with other intelligent equipment to achieve automation. Microfluidic chip has become an important approach for integrated detection of pathogens owning to miniaturization and integration in recent years. Yet there is a lack of portable methods that integrate the collection of airborne fungal spores and nucleic acid detection. Because RFSS have thick cell walls and require liquid nitrogen grinding to extract DNA, breaking the walls on-chip is difficult. Therefore, the realization of RFSS wall breaking on-chip is a major difficulty and also a very meaningful study. This study uses RFSS as the research object and provides a novel method of culturing RFSS on-chip to solve the problem of hard wall breaking, realizing the integrated detection of RFSS. The mycelium grown by RFSS germination could be easily broken to release DNA for on-chip detection, which eliminates the need for manual DNA extraction and resolves the issue of difficult wall breaking. This chip can collect RFSS based on the aerodynamic theory and achieve gas-liquid coupling through a simple microvalve structure. A micromixer is constructed to mix the liquid, and then accomplish detection quickly by recombinase polymerase amplification and lateral flow dipsticks (RPA-LFD). The detection sensitivity of this method is 1 × 102-1 × 105 CFU ml-1. It can realize the "sample in and answer out" detection of RFSS due to its simple operation, independence from precision instruments, high sensitivity and specificity. The result shows that it can be used for the early detection of RFSS, has great application prospects and is expected to promote the development of on-site instant detection equipment.

7.
Front Plant Sci ; 13: 1044500, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36352873

RESUMO

Leaf senescence, the final stage of leaf development, is one of the adaptive mechanisms formed by plants over a long period of evolution. Leaf senescence is accompanied by various changes in cell structure, physiological metabolism, and gene expressions. This process is controlled by a variety of internal and external factors. Meanwhile, the genes and plant hormones involved in leaf aging affect the quality, yield and stress resistance in horticultural plants. Leaf senescence mediated by plant hormones affected plant quality at both pre-harvest and post-harvest stages. Exogenous plant growth regulators or plant hormone inhibitors has been applied to delay leaf senescence. Modification of related gene expression by over-expression or antisense inhibition could delay or accelerate leaf senescence, and thus influence quality. Environmental factors such as light, temperature and water status also trigger or delay leaf senescence. Delaying leaf senescence could increase chloroplast lifespan and photosynthesis and thus improve source strength, leading to enhanced yield. Accelerating leaf senescence promotes nutrient redistribution from old leaves into young leaves, and may raise yield under certain circumstances. Many genes and transcriptional factors involved in leaf senescence are associated with responses to abiotic and biotic stresses. WRKY transcriptional factors play a vital role in this process and they could interact with JA signalling. This review summarized how genes, plant hormones and environmental factors affect the quality, yield. Besides, the regulation of leaf senescence holds great promise to improving the resistance to plant biotic and abiotic stresses.

8.
Adv Healthc Mater ; : e2201984, 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36394091

RESUMO

The functional blood-brain barrier (BBB) model can provide a reliable tool for better understanding BBB transport mechanisms and in vitro preclinical experimentation. However, recapitulating microenvironmental complexities and physiological functions in an accessible approach remains a major challenge. Here, we present a new BBB model with a high-cell spatial density and tightly connected biomimetic mini-tissue. The mini-tissue, pivotal functional structure of the BBB model, is fabricated by a novel and easy-to-use liquid substrate culture (LSC) method, which allows cells to self-assemble and self-heal into macro-sized, tightly connected membranous mini-tissue. The mini-tissue with uniform thickness can be easily harvested in their entirety with extracellular matrix. Attributed to the tightly connected mini-tissue formed by LSC, the fabricated BBB biomimetic model had 1 to 2 orders of magnitude higher transendothelial electric resistance than the commonly reported BBB model. It also better prevented the transmission of large molecular substances, recapitulating the functional features of BBB. Furthermore, the BBB biomimetic model provides feedback regarding BBB-destructive drugs, exhibits selective transmission, and shows efflux pump activity. Overall, this model could serve as an accessible tool for life science or clinical medical researchers to enhance the understanding of human BBB and expedite the development of new brain-permeable drugs. This article is protected by copyright. All rights reserved.

9.
Eur Radiol ; 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36394603

RESUMO

OBJECTIVE: To assess and compare the diagnostic performance of gallium-68-labelled fibroblast activation protein inhibitor ([68Ga]FAPI-04) and fluorine-18 fluorodeoxyglucose ([18F]FDG) positron emission tomography/computed tomography (PET/CT) in gastrointestinal cancer. METHODS: Fifty-one patients who underwent both [18F]FDG and [68Ga]FAPI-04 PET/CT for initial staging or restaging were enrolled. Histopathological findings, typical radiological appearances, and clinical imaging follow-up were used as the reference standard. The diagnostic performance of the two tracers was calculated and compared. The maximum standardised uptake value (SUVmax), mean SUV (SUVmean), tumour-to-mediastinal blood pool ratio (TBR), and tumour-to-liver ratio (TLR) of primary and metastatic lesions were measured and compared between two imaging modalities. RESULTS: In patient-based analysis, [68Ga]FAPI-04 showed much better diagnostic sensitivity than [18F]FDG in detecting primary tumour (94.44% [17/18] vs. 61.11% [11/18]), postoperative recurrence and metastases (95.65% [22/23] vs. 69.57% [16/23]), and peritoneal carcinomatosis (100% [28/28] vs. 60.71% [17/28]) (all p < 0.05). In lesion-based analysis, [68Ga]FAPI-04 showed higher sensitivity than [18F]FDG for detecting lymph node metastases. In peritoneal carcinomatosis, the median SUVmax (12.12 vs. 7.18) and SUVmean (6.84 vs. 4.11) with [68Ga]FAPI-04 were significantly higher than those with [18F]FDG (all p < 0.005). The TBR and TLR of [68Ga]FAPI-04 were significantly higher than those of [18F]FDG for detecting primary tumour, lymph node, liver, and peritoneal metastases (all p < 0.005). Therapeutic management changed in 13 patients according to [68Ga]FAPI-04 PET/CT compared with conventional imaging. CONCLUSIONS: [68Ga]FAPI-04 is superior to [18F]FDG PET/CT for detecting primary tumour, postoperative recurrence and metastasis, and peritoneal carcinomatosis in gastrointestinal cancer. KEY POINTS: • [68Ga]FAPI-04 PET/CT showed significantly higher sensitivity than [18F]FDG PET/CT in the detection of primary tumour and postoperative recurrence and metastasis in patients with gastrointestinal carcinoma. • [68Ga]FAPI-04 PET/CT had obvious advantages over [18F]FDG PET/CT in the detection of peritoneal carcinomatosis from gastrointestinal carcinoma with a much higher FAPI uptake value, TBR, and TLR. • Although the median SUVmax and SUVmean of [68Ga]FAPI-04 were similar to those of [18F]FDG for the primary tumour, lymph node metastases, and liver metastases in gastrointestinal carcinoma, the TBR and TLR of the SUVmax and SUVmean were significantly higher on [68Ga]FAPI-04 PET/CT, causing the lesions to be displayed more clearly.

10.
Sensors (Basel) ; 22(21)2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36366094

RESUMO

Rails play a vital role in the bearing and guidance of high-speed trains, and the normal condition of rail components is the guarantee of the operation and maintenance safety. Fasteners are critical components for fixing the rails, so it is particularly important to detect whether they are in a normal state or not. The current rail-fastener detection models have some drawbacks, including poor generalization ability, large model volume and low detection efficiency. In view of this, an improved YoLoX-Nano rail-fastener-defect-detection method is proposed in this paper. The CA attention mechanism is added to the three output feature maps of CSPDarknet and the enhanced feature extraction part of the Path Aggregation Feature Pyramid Network (PAFPN); the Adaptively Spatial Feature Fusion (ASFF) is added after the PAFPN output feature map, which enables the semantic information of the high-level features and the fine-grained features of the bottom layer to be further enhanced. The improved YoLoX-Nano model has improved the AP value by 27.42% on fractured fasteners, 15.88% on displacement fasteners and 12.96% on normal fasteners. Moreover, the mAP value is improved by 18.75%, and it is 14.75% higher than the two-stage model Faster-RCNN on mAP. In addition, compared with YoLov7-tiny, the improved YoLoX-Nano model achieves 13.56% improvement on mAP. Although the improved model increases a certain amount of calculation, the detection speed of the improved model has been increased by 30.54 fps and by 32.33 fps when compared with that of the Single-Shot Multi-Box Detector (SSD) model and the You Only Look Once v3 (YoLov3) model, reaching 54.35 fps. The improved YoLoX-Nano model enables accurate and rapid identification of the defects of rail fasteners, which can meet the needs of real-time detection. Furthermore, it has advantages in lightweight deployment of terminals for rail-fastener detection, thus providing some reference for image recognition and detection in other fields.

11.
Food Sci Nutr ; 10(11): 3772-3780, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36348798

RESUMO

The grade of green tea indicates its intrinsic quality and guides consumers when purchasing. Simple, accessible, and on-site determination of green tea grades is essential for consumers and regulators. In this study, we assumed that the turbidity difference in green tea might indicate its grade, and our results confirmed this hypothesis. The turbidity difference was measured in green tea infusions before and after the Ca2+ acceleration. For the same kind of green tea, it was found that higher grades of green tea had larger turbidity differences. Effects of brewing temperature, brewing time, Ca2+ concentration, and Ca2+ treatment time on the turbidity of green tea infusions were analyzed, and their optimal values were obtained. This study demonstrates that applying the turbidity difference and Ca2+ acceleration could be an accessible method for the on-site determination of green tea grades.

12.
J Clin Lab Anal ; : e24720, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36347827

RESUMO

BACKGROUND: MIR31HG has been affirmed to regulate the tumorigenesis of head-neck squamous cell carcinoma (HNSC). This study aims to reveal the function of MIR31HG in nasopharyngeal carcinoma (NPC), which falls into the category of HNSC. METHODS: MIR31HG expression pattern in HNSC tissues was predicted by starBase. FISH and qRT-PCR were employed to detect MIR31HG expression in NPC tissues and to analyze the association between MIR31HG and clinicopathological features. NPC cell viability, colony formation, and apoptosis were measured by MTT assay, colony formation assay, and flow cytometry. The expressions of protein kinase B (AKT), phosphorylated (p)-AKT, phosphoinositide 3-kinases (PI3K) and p-PI3K in NPC cells were analyzed by Western blot. The correlation between MIR31HG expression and AKT1 mRNA expression was analyzed by The Cancer Genome Atlas and starBase. RESULTS: MIR31HG was highly expressed in HNSC tissues and NPC tissues. Meanwhile, the association between high MIR31HG expression and aggressive clinicopathological traits was significant in NPC patients at tumor stage III-IV (T3-T4) and in those with lymph node metastasis 1-2 (N1-N2). Silencing of MIR31HG suppressed NPC cell viability and colony formation, promoted apoptosis, and decreased the expressions of p-PI3K, and p-AKT. 740Y-P reversed the above effects of si-MIR31HG on NPC cells. Besides, MIR31HG expression was positively correlated with AKT1 mRNA expression in HNSC patients. CONCLUSION: MIR31HG silencing promotes NPC cell proliferation and inhibits apoptosis through suppressing the PI3K/AKT signaling pathway.

13.
Cancer Med ; 2022 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-36341686

RESUMO

BACKGROUND: Plasma and tissue biopsy have both used for targeting actionable driver gene mutations in lung cancer, whose concordance is imperfect. A reliable method to predict the concordance is urgently needed to ease clinical application. METHODS: A total of 1012 plasma samples, including 519 with paired-tissue biopsy samples, derived from lung adenocarcinoma patients were retrospectively enrolled. We assessed the associations of several clinicopathological characteristics and serum tumor markers with the concordance between plasma and tissue biopsies. RESULTS: When carcinoembryonic antigen (CEA) levels were higher than thresholds of 15.01 ng/ml and 51.15 ng/ml, the positive predictive value of concordance reached 90% and 95%, respectively. When CEA levels were lower than thresholds of 5.19 ng/ml and 3.26 ng/mL, the negative predictive value of concordance reached 45% and 50%. The performance of CYFRA21-1 in predicting concordance was similar but inferior to CEA (AUC: 0.727 vs. 0.741, p = 0.633). The performance of CEA combined with CYFRA21-1 in predicting the concordance was similar to that of the combination of independent factors derived from the LASSO regression model (AUC: 0.796 vs. 0.818, p = 0.067). CEA (r = 0.47, p < 0.01) and CYFRA21-1 levels (r = 0.45, p < 0.05) were significantly correlated with the maximum variant allele frequency, respectively. CONCLUSIONS: CEA combined with CYFRA21-1 could effectively predict the concordance between plasma and tissue biopsies, which could be used for evaluating the priority of plasma and tissue biopsies for gene testing to timely guide clinical applications in advanced lung adenocarcinoma patients.

14.
Clin Med Insights Oncol ; 16: 11795549221134735, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387609

RESUMO

Background: Although the clinical application of osimertinib, a third-generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), has been a new step forward in the first-line treatment of non-small cell lung cancer (NSCLC), an increasing number of patients with progression on osimertinib represents a great challenge clinically. The patterns of resistance mechanisms and subsequent treatment strategies after first-line osimertinib resistance are not well established. Methods: Between January 1, 2016 and October 31, 2020, a consecutive of 56 EGFR-mutant lung cancer patients treated with osimertinib as first-line therapy at Daping Hospital (Chongqing, China) were retrospective screened. The samples of pre-osimertinib and osimertinib-resistance were all detected by next-generation sequencing (NGS) panels. Statistical analyses were carried out using SPSS 23.0 software. Survival analyses were performed using the Kaplan-Meier method and compared using a log-rank test between groups. Results: Among 47 patients with osimertinib effectiveness analysis, the median progression free survival (mPFS) was 15.4 months (95% confidence interval [CI]: 12.2-24.9 months), and median overall survival (mOS) was 35.5 months (95% CI: 23.9 months -NA). A total of 21 patients underwent repeated NGS tests upon osimertinib resistance. MET amplification was the most common resistance mechanism (6/21, 28.6%), followed by C797S mutation (5/21, 23.8%). A total of 15 patients received subsequent treatments, with mPFS of 7.3 months (95% CI 5.0 months -NA). Among them, 7 patients with EGFR C797 S or/and MET amplification received subsequent second-line targeted therapy, achieving mPFS of 7.3 months (95% CI 4.5 months -NA). Of note, 3 patients received immunotherapy as second- or third-line treatment after osimertinib resistance, achieving median clinical benefit of 37.3 months. Conclusions: MET amplification and C797S mutation are main resistance mechanisms, which could be targeted by crizotinib and gefitinib, respectively. More than 50% patients could receive subsequent anticancer targetable therapies after first-line osimertinib resistance. Immunotherapy may also be an acceptable choice after osimertinib resistance.

15.
Front Aging Neurosci ; 14: 1029533, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36389078

RESUMO

Astrocytic Ca2+ transients are essential for astrocyte integration into neural circuits. These Ca2+ transients are primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, and endfeet. In previous studies, it suggests that aging causes functional defects in astrocytes. Until now, it was unclear whether and how aging affects astrocytic Ca2+ transients at subcellular domains. In this study, we combined a genetically encoded Ca2+ sensor (GCaMP6f) and in vivo two-photon Ca2+ imaging to determine changes in Ca2+ transients within astrocytic subcellular domains during brain aging. We showed that aging increased Ca2+ transients in astrocytic primary branches, higher-order branchlets, and terminal leaflets. However, Ca2+ transients decreased within astrocytic endfeet during brain aging, which could be caused by the decreased expressions of Aquaporin-4 (AQP4). In addition, aging-induced changes of Ca2+ transient types were heterogeneous within astrocytic subcellular domains. These results demonstrate that the astrocytic Ca2+ transients within subcellular domains are affected by aging differently. This finding contributes to a better understanding of the physiological role of astrocytes in aging-induced neural circuit degeneration.

16.
Lipids Health Dis ; 21(1): 120, 2022 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-36376895

RESUMO

BACKGROUND: The relationship between remnant cholesterol (RC) and atherosclerotic cardiovascular risk has been given increasing attention in recent years. However, its association with verbal learning and memory performance has not been reported. METHODS: Data were extracted from the National Health and Nutrition Examination Survey (NHANES) 2011-2014 database. Participants aged ≥60 years with available fasting lipid data were included. Verbal learning and memory performance were evaluated using the Consortium to Establish a Registry for Alzheimer's Disease Word List Memory Task (CERAD-WL) subtest. The CERAD total score was calculated as the mean of three immediate recalls and a delayed recall. RC was calculated as total cholesterol (TC) minus the sum of low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C). Multivariate ordinal logistic regression was performed to evaluate the association between RC, as well as its derived marker, the TC/RC ratio, and age-stratified quartiles of the CERAD total score. RESULTS: A total of 1377 participants were analysed. On a continuous scale, per 1 mmol/L increase in RC and per 1 unit increase in the TC/RC ratio were associated with multivariable adjusted odds ratios (95% CI) of 0.74 (0.58-0.94) and 1.45 (1.13-1.87), respectively, for having a CERAD total score in a higher quartile. On a categorical scale, higher RC quartiles were associated with a CERAD total score in a lower quartile; in contrast, the higher TC/RC quartile was associated with a CERAD total score in a higher quartile (all P for trend < 0.05). CONCLUSIONS: The current study suggests that lower RC levels and a higher TC/RC ratio are associated with better verbal learning and memory function, which indicates that lowering RC levels could be beneficial for preventing cognitive impairment in elderly individuals. Further research is needed to validate the causal roles of RC and the TC/RC ratio in cognition.


Assuntos
Disfunção Cognitiva , Aprendizagem Verbal , Humanos , Idoso , Inquéritos Nutricionais , Cognição , Colesterol
17.
Sci Total Environ ; 858(Pt 3): 159921, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36343826

RESUMO

Substituted polycyclic aromatic hydrocarbons (SPAHs) have shown higher health and ecological risks than the corresponding parent PAHs (PPAHs) from laboratory studies, their variations in freshwater system, especially in surface sediment and pore water, remain inadequate understanding. In this study, we revealed the coexistence, ecological risk, and multiple factors affecting variations and sources of PPAHs and SPAHs (nitrated PAHs (NPAHs), oxygenated PAHs (OPAHs)) in the surface sediment-pore water system from a typical urban river in Northern China. The concentration ranges of Σ26PPAHs, Σ10NPAHs, and Σ4OPAHs in the surface sediments were 153.0-5367.4, not detected (N.D.)-105.4, and 42.2-1177.0 ng·g-1 dry weight, and fell within 0.6-38.8, N.D.-297.9, and N.D.-212.6 ng·mL-1 in the pore waters. The t-distributed stochastic neighbor embedding (SNE) coupled with the partitioning around medoids (PAM) elucidated spatiotemporal the variations in PAHs, emphasizing the impacts of industrial activities and sewage discharges. Besides the geochemical and hydrochemical conditions, SPAHs were affected by the potential secondary formation, especially during the wet season. The method comparisons indicated the advantages of principal component analysis-multivariate linear regression (PCA-MLR) and n-alkanes model on source identification. PAHs mainly originated from fossil fuel combustion and vehicular exhaust. The top risk quotient (RQ) values for PAHs occurred in the urban and industrial sections. A majority of the surface sediment samples emerged with low to moderate exposure risks, while all the pore water samples showed high exposure risks. The RQs of OPAHs were significantly higher (p < 0.01) than those of PPAHs. The results suggested the secondary formation of SPAHs as an important role in ecological risks of PAHs in the urban river system.

18.
Int J Bioprint ; 8(4): 619, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36404784

RESUMO

Vessel-on-a-chips, which can be used to study microscale fluid dynamics, tissue-level biological molecules delivery and intercellular communication under favorable three-dimensional (3D) extracellular matrix microenvironment, are increasingly gaining traction. However, not many of them can allow for long-term perfusion and easy observation of angiogenesis process. Since angiogenesis is necessary for the expansion of tumor, antiangiogenic drugs play a significant role in cancer treatment. In this study, we established an innovative and reliable antiangiogenic drug screening chip that was highly modularly integrated for long-term perfusion (up to 10 days depending on the hydrogel formula) and real-time monitoring. To maintain an unobstructed flow of cell-laden tubes for subsequent perfusion culture on the premise of excellent bioactivities, a polycaprolactone stent inspired by coronary artery stents was introduced to hold up the tubular lumen from the inside, while the perfusion chip was also elaborately designed to allow for convenient observation. After 3 days of perfusion screening, distinct differences in human umbilical vein endothelial cell sprouting were observed for a gradient of concentrations of bevacizumab, which pointed to the effectiveness and reliability of the drug screening perfusion system. Overall, a perfusion system for antiangiogenic drug screening was developed, which can not only conduct drug evaluation, but also be potentially useful in other vessel-mimicking scenarios in the area of tissue engineering, drug screening, pharmacokinetics, and regenerative medicine.

19.
Int J Bioprint ; 8(4): 599, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36404788

RESUMO

Centimeter-scale tissue with angiogenesis has become more and more significant in organ regeneration and drug screening. However, traditional bioink has obvious limitations such as balance of nutrient supporting, printability, and vascularization. Here, with "secondary bioprinting" of printed microspheres, an innovative bioink system was proposed, in which the thermo-crosslinked sacrificial gelatin microspheres encapsulating human umbilical vein endothelial cells (HUVECs) printed by electrospraying serve as auxiliary component while gelatin methacryloyl precursor solution mixed with subject cells serve as subject component. Benefiting from the reversible thermo-crosslinking feature, gelatin microspheres would experience solid-liquid conversion during 37°C culturing and form controllable porous nutrient network for promoting the nutrient/oxygen delivery in large-scale tissue and accelerate the functionalization of the encapsulated cells. Meanwhile, the encapsulated HUVECs would be released and attach to the pore boundary, which would further form three-dimensional vessel network inside the tissue with suitable inducing conditions. As an example, vascularized breast tumor tissue over 1 cm was successfully built and the HUVECs showed obvious sprout inside, which indicate the great potential of this bioink system in various biomedical applications.

20.
Foods ; 11(21)2022 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-36360109

RESUMO

OBJECTIVE: Walnuts have rich nutritional value and are favored by the majority of consumers. As walnuts are shelled nuts, they are prone to suffer from defects such as mildew during storage. The fullness and mildew of the fruit impose effects on the quality of the walnuts. Therefore, it is of great economic significance to carry out a study on the rapid, non-destructive detection of walnut quality. METHODS: Terahertz spectroscopy, with wavelengths between infrared and electromagnetic waves, has unique detection advantages. In this paper, the rapid and nondestructive detection of walnut mildew and fullness based on terahertz spectroscopy is carried out using the emerging terahertz transmission spectroscopy imaging technology. First, the normal walnuts and mildewed walnuts are identified and analyzed. At the same time, the image processing is carried out on the physical samples with different kernel sizes to calculate the fullness of the walnut kernels. The THz image of the walnuts is collected to extract the spectral information in different regions of interest. Four kinds of time domain signals in different regions of interest are extracted, and three qualitative discrimination models are established, including the support vector machine (SVM), random forest (RF), and k-nearest neighbor (KNN) algorithms. In addition, in order to realize the visual expression of walnut fullness, the terahertz images of the walnut are segmented with a binarization threshold, and the walnut fullness is calculated by the proportion of the shell and kernel pixels. RESULTS: In the frequency domain signal, the amplitude intensity from high to low is the mildew sample, walnut kernel, and walnut shell, and the distinction between walnut kernel, shell samples, and mildew samples is high. The overall identification accuracy of the aforementioned three models is 90.83%, 97.38%, and 97.87%, respectively. Among them, KNN has the best qualitative discrimination effect. In a single category, the recognition accuracy of the model for the walnut kernel, walnut shell, mildew sample, and reference group (background) reaches 94%, 100%, 97.43%, and 100%, respectively. The terahertz transmission images of the five categories of walnut samples with different kernel sizes are processed to visualize the detection of kernel fullness inside walnuts, and the errors are less than 5% compared to the actual fullness of walnuts. CONCLUSION: This study illustrates that terahertz spectroscopy detection can achieve the detection of walnut mildew, and terahertz imaging technology can realize the visual expression and fullness calculation of walnut kernels. Terahertz spectroscopy and imaging provides a non-destructive detection method for walnut quality, which can provide a reference for the quality detection of other dried nuts with shells, thus having significant practical value.

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