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1.
ACS Nano ; 17(1): 472-491, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36574627

RESUMO

Calcium overload is one of the early determinants of the core cellular events that contribute to the pathogenesis of acute kidney injury (AKI), which include oxidative stress, ATP depletion, calcium overload, and inflammatory response with self-amplifying and interactive feedback loops that ultimately lead to cellular injury and renal failure. Excluding adjuvant therapy, there are currently no approved pharmacotherapies for the treatment of AKI. Using an adipic dihydride linker, we modified the hyaluronic acid polymer chain with a potent antioxidant, bilirubin, to produce an amphiphilic conjugate. Subsequently, we developed a kidney-targeted and reactive oxygen species (ROS)-responsive drug delivery system based on the flash nanocomplexation method to deliver a well-known intracellular calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM, BA), with the goal of rescuing renal cell damage via rapidly scavenging of intracellularly overloaded Ca2+. In the ischemia-reperfusion (I/R) induced AKI rat model, a single dose of as-prepared formulation (BA 100 µg·kg-1) 6 h post-reperfusion significantly reduced renal function indicators by more than 60% within 12 h, significantly alleviated tissular pathological changes, ameliorated tissular oxidative damage, significantly inhibited apoptosis of renal tubular cells and the expression of renal tubular marker kidney injury molecule 1, etc., thus greatly reducing the risk of kidney failure. Mechanistically, the treatment with BA-loaded NPs significantly inhibited the activation of the ER stress cascade response (IRE1-TRAF2-JNK, ATF4-CHOP, and ATF6 axis) and regulated the downstream apoptosis-related pathway while also reducing the inflammatory response. The BA-loaded NPs hold great promise as a potential therapy for I/R injury-related diseases.


Assuntos
Injúria Renal Aguda , Nanopartículas , Ratos , Animais , Cálcio/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Ácido Hialurônico , Bilirrubina , Apoptose , Injúria Renal Aguda/tratamento farmacológico , Estresse do Retículo Endoplasmático
2.
Poult Sci ; 102(1): 102292, 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36435165

RESUMO

The importance of thyroid-related genes has been repeatedly mentioned in the transcriptome studies of poultry with different laying performance, yet there are few systematic studies to unravel the regulatory mechanisms of the thyroid-ovary axis in the poultry egg production process. In this study, we compared the transcriptome profiles in the thyroid and ovarian stroma between high egg production (GP) and low egg production (DP) ducks, and then revealed the pathways and candidate genes involved in the process. We identified 1,114 and 733 differentially expressed genes (DEGs) in the thyroid and ovarian stroma, separately. The Gene Ontology (GO) analysis showed that a total of 504 and 189 GO terms were identified in the thyroid and ovarian stroma (P < 0.05). Three common GO terms were identified from the top 5 GO terms with the highest significant level in two tissues, including extracellular space, calcium ion binding, and integral component of plasma membrane. The enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that 15 and 14 KEGG pathways were significantly (P < 0.05) enriched in the thyroid and ovarian stroma, respectively. And, there were 8 common pathways, including neuroactive ligand-receptor interaction, calcium signaling pathway, ECM-receptor interaction, PPAR signaling pathway, melanogenesis, wnt signaling pathway, vascular smooth muscle contraction, and cytokine-cytokine receptor interaction. Notably, the neuroactive ligand-receptor interaction pathway was the most significantly enriched by the DEGs both in the thyroid and ovarian stroma. The interaction among DEGs enriched in the neuroactive ligand-receptor interaction and ECM-receptor interaction suggested that the thyroid may regulate ovarian development by these genes. Through integrated analysis of the protein-protein interaction (PPI) network and KEGG pathway maps, 9 key DEGs (PTH, THBS2, THBS4, CD36, ADIPOQ, ACSL6, PRKAA2, CRH, and PCK1) were identified, which could play crucial roles in the thyroid to regulate ovarian function and then affect egg-laying performance between GP and DP. This study serves as a basis to explore the molecular mechanism of the thyroid affecting ovarian function and egg production in female ducks and may help to identify molecular markers that can be used for duck genetic selection.

3.
Sci Adv ; 8(35): eabo0617, 2022 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-36054354

RESUMO

Extreme conditions inside ice giants such as Uranus and Neptune can result in peculiar chemistry and structural transitions, e.g., the precipitation of diamonds or superionic water, as so far experimentally observed only for pure C─H and H2O systems, respectively. Here, we investigate a stoichiometric mixture of C and H2O by shock-compressing polyethylene terephthalate (PET) plastics and performing in situ x-ray probing. We observe diamond formation at pressures between 72 ± 7 and 125 ± 13 GPa at temperatures ranging from ~3500 to ~6000 K. Combining x-ray diffraction and small-angle x-ray scattering, we access the kinetics of this exotic reaction. The observed demixing of C and H2O suggests that diamond precipitation inside the ice giants is enhanced by oxygen, which can lead to isolated water and thus the formation of superionic structures relevant to the planets' magnetic fields. Moreover, our measurements indicate a way of producing nanodiamonds by simple laser-driven shock compression of cheap PET plastics.

4.
Contrast Media Mol Imaging ; 2022: 1543604, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36176925

RESUMO

A wound is the pathological change of soft tissue under normal skin caused by various factors, such as collision, contusion, hot crush, avulsion, corrosive chemicals, operations, excessive wound tension after operations, local pressure that cannot be relieved for a long time, liquid immersion, local infection, and rejection reactions caused by allogeneic substances. The skin itself or its underlying soft tissue loses its integrity and continuity, thus losing its normal physiological function. Medical image analysis is a medical term that refers to the interdisciplinary fields of integrated medical imaging, artificial intelligence, digital image processing and analysis, mathematical modeling, and numerical algorithms. According to the time of wound formation, they can be divided into acute and chronic wounds. The common acute wounds include lacerations caused by trauma, surgical incisions, burns, and donor sites formed after skin graft operations. This article mainly studies the role of platelet-rich plasma gel nanocomposites in promoting wound healing. It is proven that ptt-rich plasma gel can significantly promote tissue repair and regeneration and accelerate wound healing in patients with severe burns. The atomic number of the nanocomposite has a better treatment effect on the nanoparticle approach. In this paper, chitosan nanocomposite membrane, nanocomposite algorithm, and the calculation method of enthalpy of formation of high alloy nanomaterials were used to study the role of ptt-rich plasma gel combined chitosan nanocomposite membrane loaded bone marrow stromal cells in promoting wound healing, and its effects were applied to the repair of special site burns, special burns, and different age burns. Good wound repair benefits from the correct treatment of the wound, which directly affects the stability and development of the internal environment. The difference in healing time between the two groups was statistically significant, and the recovery time of the PRP group was 0.001 less than that of the control group. The results showed that the wound healing time of the PRP group was significantly shorter than that of the control group (P < 0.05); after treatment, the content of VEGF in the wound tissue of the two groups increased, especially in the PRP group; the effective rate of the PRP group was 75.0%, which was higher than 68.8% of the control group. It can play an important role in the regulation of expression and the pathophysiological process of wound healing.


Assuntos
Queimaduras , Quitosana , Plasma Rico em Plaquetas , Cicatrização , Ligas , Inteligência Artificial , Queimaduras/diagnóstico por imagem , Queimaduras/terapia , Humanos , Fator A de Crescimento do Endotélio Vascular
5.
J Hazard Mater ; 438: 129454, 2022 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-35803186

RESUMO

Growing inputs of microplastics into marine sediment have increased significantly the needs for assessment of their potential risks to the marine benthos. A knowledge gap remains with regard to the effect of microplastics on benthos, such as cephalochordates. By employing amphioxus as a model benthic chordate, here we show that exposure to microplastics for 96 h at doses of 1 mg/L and 100 mg/L results in evident accumulation of the polyethylene microplastics. The accumulated microplastics are as much as 0.027% of body weight upon high-dose exposure, causing an abnormal body-bending phenotype that limits the locomotion capability of amphioxus. Mechanistic insight reveals that microplastics can bring about histological damages in gill, intestine and hepatic cecum; In-depth assay of relevant biomarkers including superoxide dismutase, catalase, glutathione, pyruvic acid and total cholesterol indicates the occurrence of oxidative damage and metabolic disorder; Further, microplastics exposure depresses the activity of acetylcholinesterase while allowing the level of acetylcholine to rise in muscle, suggesting the emergence of neurotoxicity. These consequences eventually contribute to the muscle dysfunction of amphioxus. This study rationalizes the abnormal response of the vulnerable notochord to microplastics, signifying the dilemma suffered by the ancient lineage under the emerging threat. Given the enrichment of microplastics through marine food chains, this study also raises significant concerns on the impact of microplastics to other marine organisms, and eventually human beings.


Assuntos
Anfioxos , Microplásticos , Poluentes Químicos da Água , Acetilcolinesterase , Animais , Anfioxos/efeitos dos fármacos , Microplásticos/toxicidade , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/toxicidade
6.
Inorg Chem ; 61(25): 9776-9784, 2022 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-35696486

RESUMO

Mid-infrared laser in the 2-5 µm wavelength region is in the atmospheric transmission window range, and hence, it has important application prospects in the fields of optoelectronic countermeasures, space communication, environmental remote sensing, and molecular spectroscopy. One of the most promising technological approaches to achieve mid-infrared laser output is based on direct lasing of transition-metal (TM)-doped II-VI chalcogenide crystals. In this work, CdSxSe1-x and Cr:CdS0.8Se0.2 polycrystals were synthesized by a chemical vapor synthesis method from a stoichiometric mixture of vacuum-sublimed CdS and CdSe. The structure of the synthesized products was analyzed by X-ray diffraction (XRD). Using these synthesized products, CdSxSe1-x and Cr:CdS0.8Se0.2 single crystals were grown by the physical vapor transport (PVT) method. After annealing, the band gap becomes smaller and the transmission range widens to 17 µm. The composition of the single crystals was determined by energy-dispersive spectrometry (EDS) mapping and XPS, and it was found to be uniform throughout the ingot. In addition, the absorption peak maximum for the Cr2+ ion in the Cr:CdS0.8Se0.2 crystal is at 1.84 µm.

7.
Small Methods ; 6(7): e2200139, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35587774

RESUMO

Diverse gases (NO, CO, H2 S, H2 , etc.) have been widely applied in the medical intervention of various diseases, including cancer, cardiovascular disease, ischemia-reperfusion injury, bacterial infection, etc., attributing to their inherent biomedical activities. Although many gases have many biomedical activities, their clinical use is still limited due to the rapid and free diffusion behavior of these gases molecules, which may cause potential side effects and/or ineffective treatment. Gas-generating nanoplatforms (GGNs) are effective strategies to address the aforementioned challenges of gas therapy by preventing gas production or release at nonspecific sites, enhancing GGNs accumulation at targeted sites, and controlling gas release in response to exogenous (UV, NIR, US, etc.) or endogenous (H2 O2 , GSH, pH, etc.) stimuli at the lesion site, further maintaining gas concentration within the effective range and achieving the purpose of disease treatment. This review comprehensively summarizes the advancements of "state-of-the-art" GGNs in the recent three years, with emphasis on the composition, structure, preparation process, and gas release mechanism of the nanocarriers. Furthermore, the therapeutic effects and limitations of GGNs in preclinical studies using cell/animal models are discussed. Overall, this review enlightens the further development of this field and promotes the clinical transformation of gas therapy.


Assuntos
Gases , Neoplasias , Animais , Gases/uso terapêutico , Neoplasias/tratamento farmacológico
8.
Inorg Chem ; 61(17): 6562-6573, 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35426664

RESUMO

The new quaternary single crystals AgGaGenSe2(n+1) (n = 1.5, 1.75, 2, 3, 4, 5, and 9) have high nonlinear optical property and can be used for mid-IR laser applications in high power. However, only AgGaGe3Se8 and AgGaGe5Se12 have been grown on a large scale and studied in detail. In this work, the AgGaGenSe2(n+1) (n = 1.5, 1.75, 2, 3, 4, 5, and 9) crystals (Φ 20 mm × 40 mm and Φ 40 mm × 100 mm) were grown by the modified Bridgman method. The crystal structure was studied by X-ray diffraction and the Rietveld refinement method. The composition and morphology were characterized by scanning electron microscopy and metallurgical microscopy. The chemical state and vibration modes of surface elements were characterized by X-ray photoelectron spectroscopy and Raman spectra, and the electrical property was investigated by the Hall effect measurement, which indicates that all the single AgGaGenSe2(n+1) crystals are n-type semiconductors. The transmittance of all as-grown AgGaGenSe2(n+1) crystal wafers exceed 65% in the transparent range, and the band gap increases from 2.05 eV for AgGaGe1.5Se5 to 2.14 eV for AgGaGe9Se20. Besides, after being annealed in two different conditions, the wafers show different changes. We discovered a special decomposition phenomenon during the annealing process and found the more appropriate annealing method at last. In addition, the absorption peaks at 4.2, 10, and 14.9 µm of wafers have been nearly eliminated, and the quality of most crystals has been improved.

9.
Small Methods ; 6(3): e2101391, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35107224

RESUMO

Single-cell encapsulation is an emerging technology to endow cells with various functions, of which developing new applications in vivo is in high demand. Currently, metal-organic frameworks (MOFs) that are used as nanometric shells to coat living cells, however, have not realized cell-selective encapsulation. Here, a biocompatible and selective cell encapsulation strategy based on precursor-functionalized nucleolin aptamer and in situ MOF mineralization on the aptamer-identified cancer cell surface are developed. After MOF coating, the encapsulated cancer cells undergo immunogenic cell death, which is found associated with the changed cell stiffness (indicated by Young's modulus). The immunogenic dead cancer cells are used as whole-cell cancer vaccines (WCCVs), forming the integral WCCV-in-shell structure with enhanced immunogenicity ascribing from the surface-exposed calreticulin to promote dendritic cell recruitment, antigen presentation, and T-cell activation. The major activation pathways in the immune response are identified including tumor necrosis factor signaling pathway, cytokine-cytokine receptor interaction, and Toll-like receptor signaling pathway, suggesting the potential adjuvant effect of the MOF shells. After vaccination, WCCV-in-shell shows much better tumor immunoprophylaxis than either the imperfectly coated cancer cells or the traditional WCCV. This strategy is promising for the universal and facile development of novel whole-cell vaccines.


Assuntos
Vacinas Anticâncer , Estruturas Metalorgânicas , Neoplasias , Vacinas Anticâncer/uso terapêutico , Encapsulamento de Células , Humanos , Estruturas Metalorgânicas/química , Neoplasias/tratamento farmacológico , Oligonucleotídeos/uso terapêutico
10.
Int J Pharm ; 611: 121317, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34838624

RESUMO

The preferable choice of sustained peptide delivery systems is generally polymer-based microspheres in which their large particle size, wide size distribution, low drug encapsulation efficacy, poor colloidal stability, and undesirable burst release eventually hinder their clinical translation. In this study, a nanoscale ternary Lixisenatide (Lix) sustained delivery system based on strong multivalent interactions (electrostatic and coordination complexation) among small molecular phytic acid (PA), Lix and Fe3+ was developed. Flash nanocomplexation (FNC) was utilized to facilitate the rapid and efficient mixing of the three components and kinetically control the assembly process that enabled dynamic balance of two competitive chemical reactions with different kinetic rates (slow chemical reaction of PA/Lix and fast chemical reaction of PA/Fe3+) to generate structural uniform ternary nanoparticles and avoid heterogeneous complexes. By tuning the mixing conditions (i.e., flow rate, mass ratio, concentration, pH value, etc.), the ternary PA/Lix/Fe3+ nanoparticles were assembled with reproducible production in a manner of high uniformity and scalability, achieving small size (∼50 nm), uniform composition (PDI: ∼0.12), favourable colloidal stability, high encapsulation efficiency (∼100%), and tunable drug release kinetics. The optimized formulation exhibited a minor Lix release (<20%) in the first day and extended peptide release period over 8 days. Unexpectedly, upon a single injection administration, the as-prepared formulation (600 µg/kg) rapidly brought the high BGL (∼30 mmol/L) back to normal range (<10 mmol/L) within the initial 6 h and achieved a 180 h glycemic control in T2D mouse model. Moreover, this sustained peptide delivery system demonstrated a repeatable hypoglycemic effects and significantly suppressed the pathological damage of major organs following multiple injection. This sustained peptide delivery system with aqueous, facile and reproducible preparation process possesses good biocompatibility, tunable release kinetics, and prolonged hypoglycemic effects, portending its great translational potential in the chronic disease treatment.


Assuntos
Nanopartículas , Ácido Fítico , Animais , Cinética , Camundongos , Peptídeos
11.
Int J Cardiol ; 345: 83-88, 2021 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-34653575

RESUMO

OBJECTIVE: Improving health-related quality of life (HRQoL) and exercise capacity is an important goal of treatment in heart failure (HF). However, evidence for the effects of sodium-glucose cotransporter-2 (SGLT-2) inhibitors on the improvement of HRQoL and exercise capacity seems to be conflicted. We performed a systematic review and meta-analysis to evaluate the effects of SGLT-2 inhibitors on HRQL and exercise capacity in patients with heart failure and reduced ejection fraction (HFrEF). METHODS: All studies (up to March 20, 2021) evaluating the effects of SGLT-2 inhibitors on HRQoL and exercise capacity in patients with HFrEF were initially searched from four electronic search engines: PubMed, Web of Science, Cochrane Library, and SinoMed. All statistical analyses were performed with RevMan 5.4. RESULTS: We included 9 articles describing 7 trials with 9428 patients. SGLT-2 inhibitors group exhibited significant improvement in HRQoL assessed by Kansas City Cardiomyopathy Questionnaires (KCCQ) (MD: 2.13, 95% CI: 1.11 to 3.14, p < 0.001) and the rate of KCCQ-overall summary score improvement≥5 points (RR 1.15, 95%CI 1.08 to 1.21, P < 0.001) compared with placebo. No significant difference was observed in exercise capacity assessed by 6-min walk test distance between SGLT-2 inhibitors and placebo (MD 24.45, 95%CI -22.82 to 71.72, P = 0.31). CONCLUSIONS: Our meta-analysis demonstrates that SGLT-2 inhibitors significantly improve HRQoL, and supports the concept that SGLT-2 inhibitors do not significantly improve exercise capacity in patients with HFrEF. Studies with larger sample sizes and longer follow-up duration are needed to determine whether the treatment with SGLT-2 inhibitors may improve exercise ability. PROSPERO: CRD42021248346.


Assuntos
Insuficiência Cardíaca , Inibidores do Transportador 2 de Sódio-Glicose , Tolerância ao Exercício , Insuficiência Cardíaca/tratamento farmacológico , Humanos , Qualidade de Vida , Volume Sistólico
12.
Front Pharmacol ; 12: 649398, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34335241

RESUMO

Long non-coding RNA (lncRNA) is widely reported to be involved in cardiac (patho)physiology. Acute myocardial infarction, in which cardiomyocyte apoptosis plays an important role, is a life-threatening disease. Here, we report the lncRNA Chaer that is anti-apoptotic in cardiomyocytes during Acute myocardial infarction. Importantly, lncRNA Chaer is significantly downregulated in both oxygen-glucose deprivation (oxygen-glucose deprivation)-treated cardiomyocytes in vitro and AMI heart. In vitro, overexpression of lncRNA Chaer with adeno virus reduces cardiomyocyte apoptosis induced by OGD-treated while silencing of lncRNA Chaer increases cardiomyocyte apoptosis instead. In vivo, forced expression of lncRNA Chaer with AAV9 attenuates cardiac apoptosis, reduces infarction area and improves mice heart function in AMI. Interestingly, overexpression of lncRNA Chaer promotes the phosphorylation of AMPK, and AMPK inhibitor Compound C reverses the overexpression of lncRNA Chaer effect of reducing cardiomyocyte apoptosis under OGD-treatment. In summary, we identify the novel ability of lncRNA Chaer in regulating cardiomyocyte apoptosis by promoting phosphorylation of AMPK in AMI.

13.
Inorg Chem ; 60(15): 11098-11109, 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34269566

RESUMO

Quaternary nonlinear optical single crystals AgxGaxGe1-xSe2 (x = 0.250, 0.167) were grown by the Bridgman method in a four-zone furnace. The thermal expansion behavior of AgxGaxGe1-xSe2 (x = 0.25, 0.167) was studied by the method of single-crystal X-ray diffraction from 150 to 295 K and powder X-ray diffraction in the range of 298-773 K. Both results show the crystals have positive linear thermal expansion coefficients in different directions and a positive volume thermal expansion coefficient, and it is observed that they satisfy the relationship of αa > αc > αb and αV ≈ αa + αb + αc for the orthorhombic structure. It is found that the AgxGaxGe1-xSe2 (x = 0.25, 0.167) unit cells varying with temperature were mainly dominated by variations in framework geometry (AgSe4 tetrahedron), and the thermal motion of Ag atoms in the AgSe4 tetrahedron. As it was revealed, according to the powder X-ray diffraction, it is found that the isotropic thermal atomic displacement parameter of the Ag atoms is much larger than those of the Se and Ga(Ge) atoms in the AgSe4 tetrahedron. Furthermore, anisotropic atomic displacement parameters (ADPs) of Ag atoms are extracted from the single-crystal diffraction; the ADPs along the a axis, b axis, and c axis have a significant difference, which means the thermal vibration of Ag atoms is anisotropic. It is of great significance for improving crystal growth technology and understanding the thermal properties of this kind of crystals.

14.
Nanoscale ; 13(24): 10726-10747, 2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34165483

RESUMO

Sepsis is a life threatening disease that is caused by a dysregulated host immune response to infection, resulting in tissue damage and organ dysfunction, which account for a high in-hospital mortality (approximately 20%). However, there are still no effective and specific therapeutics for clinical sepsis management. Nanomaterial-based strategies have emerged as promising tools for improving the therapeutic efficacy of sepsis by combating lethal bacterial infection, modulating systemic inflammatory response, preventing multiple organ failure, etc. This review has comprehensively summarized the recent advancements in nanomaterial-based strategies for the management of sepsis and severe complications, in which those nanosystems act either as inherent therapeutics or as nanocarriers for the precise delivery of agents. These formulations mechanically possess antibacterial, anti-inflammatory, immunomodulatory, and anti-oxidative effects, achieving multifunctional synergistic treatment efficacy against sepsis. Furthermore, several cell membrane-derived biomimetic nanoplatforms have been used as decoys to trap and neutralize the pathogenic toxins. The critical role of other adjuvant therapies in sepsis management, including the combination of nanotechnology and stem cell therapy, is also highlighted. Overall, this review provides insights into innovative nanotechnology-based strategies applied in sepsis treatment.


Assuntos
Nanoestruturas , Sepse , Anti-Inflamatórios/uso terapêutico , Humanos , Imunidade , Imunomodulação , Sepse/tratamento farmacológico
15.
J Control Release ; 336: 233-251, 2021 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-34171444

RESUMO

Acute kidney injury (AKI), a major health issue concerning ~50% of patients treated in intensive care units, generally leads to severe renal damage associated with high mortality rate. The application of nanotechnology for the management of AKI has profound potential of further development, providing innovative strategies for predicting the early onset and progression of renal disease and improving the treatment efficacy of the life-threating AKI. This review has comprehensively summarized the nanomedicines in the application of AKI diagnosis and emphatically discussed the unique potential of various nanotechnology-based drug delivery systems (e.g., polymeric nanoparticles, organic nanoparticles, inorganic nanoparticles, lipid-based nanoparticles, hydrogels etc.) in the treatment of AKI, allowing for improved therapeutic index by enhancing both efficacy and safety concurrently. These approaches may mechanically mitigate oxidative stress, inflammation, and mitochondrial and other organellar damage, etc. In addition, the combination of nanotechnology with stem cells-based therapy or gene therapy has been explored for reducing renal tissues damage and promoting kidney repair or recovery from AKI. The review provides insights into the synthesis, advantages, and limitations of innovative nanomedicine application in the early detection and effective treatment of AKI.


Assuntos
Injúria Renal Aguda , Injúria Renal Aguda/diagnóstico , Injúria Renal Aguda/tratamento farmacológico , Sistemas de Liberação de Medicamentos , Humanos , Rim , Nanomedicina , Nanotecnologia
16.
Pacing Clin Electrophysiol ; 44(7): 1151-1162, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33990979

RESUMO

BACKGROUND: Contemporary radiofrequency catheter ablation (RFCA) approaches for atrial fibrillation (AF) have reached an efficacy "ceiling". Ethanol infusion into the vein of Marshall (EI-VOM) has shown potential in preliminary studies. Data on EI-VOM are largely limited to small single-center reports, and clinical benefits and risks have not been systematically examined. Therefore, we performed a meta-analysis to assess the feasibility, efficacy, and safety of EI-VOM for AF. METHODS: All studies evaluating EI-VOM for AF were initially searched from four electronic search engines: PubMed, Web of Science, Cochrane Library, and SinoMed. We used RevMan5.4 to calculate pooled outcomes of randomized controlled trial and cohort studies. We also performed single-arm meta-analyses using Open Meta-Analyst. RESULTS: We included a total of 10 studies with 1322 patients. Successful EI-VOM was performed in 86.7% (95% CI 81.9-91.4%) of patients. For persistent AF patients, the recurrence of AF and/or atrial tachycardia (AT) was significantly lower in the EI-VOM combined with RFCA group compared with RFCA alone group (RR 0.58, 95% CI 0.35 to 0.96, p = 0.04). EI-VOM combined with RFCA significantly increased the rate of bidirectional mitral isthmus block compared with RFCA alone in AF patients (RR 1.50, 95% CI 1.34 to 1.67, p < 0.001). There were nine cardiac tamponades observed in 644 patients (PR 0.8%, 95% CI 0.1-1.5%) who were performed EI-VOM combined with RFCA. CONCLUSIONS: Our meta-analysis brings encouraging evidence that adjuvant EI-VOM reduces AF and/or AT recurrence rate in persistent AF patients and increases the success rate of bidirectional mitral isthmus block.


Assuntos
Fibrilação Atrial/tratamento farmacológico , Etanol/administração & dosagem , Etanol/efeitos adversos , Estudos de Viabilidade , Humanos , Infusões Intravenosas , Resultado do Tratamento , Veias
17.
Biomacromolecules ; 22(6): 2299-2324, 2021 06 14.
Artigo em Inglês | MEDLINE | ID: mdl-33957752

RESUMO

Peptide/protein therapeutics have been significantly applied in the clinical treatment of various diseases such as cancer, diabetes, etc. owing to their high biocompatibility, specificity, and therapeutic efficacy. However, due to their immunogenicity, instability stemming from its complex tertiary and quaternary structure, vulnerability to enzyme degradation, and rapid renal clearance, the clinical application of protein/peptide therapeutics is significantly confined. Though nanotechnology has been demonstrated to prevent enzyme degradation of the protein therapeutics and thus enhance the half-life, issues such as initial burst release and uncontrollable release kinetics are still unsolved. Moreover, the traditional administration method results in poor patient compliance, limiting the clinical application of protein/peptide therapeutics. Exploiting the sustained-release formulations for more controllable delivery of protein/peptide therapeutics to decrease the frequency of injection and enhance patient compliance is thus greatly meaningful. In this review, we comprehensively summarize the substantial advancements of protein/peptide sustained-release systems in the past decades. In addition, the advantages and disadvantages of all these sustained-release systems in clinical application together with their future challenges are also discussed in this review.


Assuntos
Sistemas de Liberação de Medicamentos , Peptídeos , Preparações de Ação Retardada , Meia-Vida , Humanos , Proteínas
18.
Analyst ; 146(13): 4154-4160, 2021 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-33977966

RESUMO

Self-assembly performed in ionic liquids (ILs) as a unique solvent promises distinct functions and applications in sensors, therapeutics, and optoelectronic devices due to the rich interactions between nanoparticle building blocks and ILs. However, the general consideration that common nanoparticles are readily destabilized by counterions in an IL has largely prevented researchers from investigating controlled nanoparticle assembly in IL-based systems. This study explores the assembling behaviour of double-stranded (ds) DNA-functionalized gold nanoparticles (dsDNA-AuNPs) in hydrated ionic liquids. The DNA base pair stacking assembly of dsDNA-AuNPs occurs at a low IL concentration (<5%). However, a moderate ionic liquid concentration (5-40%) can de-hybridize dsDNA and leaves single-stranded (ss) DNA stabilizing the AuNPs. In concentrated ionic liquids (>40%), interestingly, the higher ionic strength leads to the assembly of DNA-AuNPs. The triphasic assembly trend is also generally observed regardless of the type of IL. By down-regulation of DNA's melting temperature with the IL, the assembly of DNA-AuNPs affords robust response to a lower temperature range, promising applications in plasmonic devices and range-tunable temperature sensors.


Assuntos
Líquidos Iônicos , Nanopartículas Metálicas , DNA , DNA de Cadeia Simples , Ouro
19.
Inorg Chem ; 60(10): 7345-7350, 2021 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-33902276

RESUMO

Second-harmonic generation (SHG) is a kind of nonlinear optical phenomenon which has been widely used in optical devices, and factors influencing its signal are very complex. Here, taking advantage of excellent structural designability and overcoming the limitations of various coordinations of lanthanide metals, for the first time a series of lanthanide metal-organic frameworks (Ln-MOFs) with one particular ligand were synthesized and structurally characterized to study the interference of the SHG signal. The optical performance including single-photon fluorescence and SHG was collected and analyzed. It is found that all 13 kinds of Ln-MOFs can be divided into 2 crystal configurations by their individual space groups and Ln-MOFs with coordinated metal atoms from La to Tb possessing the noncentrosymmetric C2 space group exhibit the SHG property, the intensity of which depends on the type of metal atoms, the pumping wavelength, and the size of the single-crystal particles. This is the first time that the relationship between the nonlinear optical properties and the structure, metal atoms, pumping wavelength, crystal size of the whole series of Ln-MOFs is studied systematically, providing a lot of interesting results and enriching the research scope of nonlinear optics and materials science.

20.
Sci Rep ; 11(1): 6116, 2021 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-33731787

RESUMO

The sound velocities of water in the Hugoniot states are investigated by laser shock compression of precompressed water in a diamond anvil cell. The obtained sound velocities in the off-Hugoniot region of liquid water at precompressed conditions are used to test the predictions of quantum molecular dynamics (QMD) simulations and the SESAME equation-of-state (EOS) library. It is found that the prediction of QMD simulations agrees with the experimental data while the prediction of SESAME EOS library underestimates the sound velocities probably due to its improper accounting for the ionization processes.

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