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J Vet Med Sci ; 83(9): 1378-1388, 2021 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-34248104


Epizootic hemorrhagic disease (EHD) is an arthropod-borne disease of wild and domestic ruminants caused by the EHD virus (EHDV). To date, seven EHDV serotypes have been identified. In Japan, strain Ibaraki of EHDV serotype 2 has caused outbreaks of Ibaraki disease in cattle. In addition, EHDV serotype 7 (EHDV-7) has caused large-scale EHD epizootics. In mid-September 2016, eight cattle at a breeding farm in Fukuoka Prefecture, Japan developed fever. Since EHDV-7 was detected in sentinel cattle in western Japan in 2016, we suspected that the cause of this fever might be an EHDV-7 infection. In this study, we tested cattle for EHDV-7 and some other viruses. Consequently, EHDV was isolated from washed blood cells collected from three of the eight cattle, and genetic analysis of genome segment 2 revealed that this isolate was EHDV-7. Moreover, all affected cattle tested positive for anti-EHDV-7 neutralizing antibodies. Our results suggest that the fever was caused by EHDV-7 infection. In addition, we modified a conventional reverse transcription polymerase chain reaction assay for the specific detection of EHDV. This modified assay could detect various strains of EHDV isolated in Japan, Australia, and North America. Furthermore, the assay permitted the detection of EHDV-7 in blood cells collected from seven of the eight cattle. We believe that this modified assay will be a useful tool for the diagnosis of EHD.

Doenças dos Bovinos , Vírus da Doença Hemorrágica Epizoótica , Infecções por Reoviridae , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Vírus da Doença Hemorrágica Epizoótica/genética , Japão/epidemiologia , Infecções por Reoviridae/diagnóstico , Infecções por Reoviridae/epidemiologia , Infecções por Reoviridae/veterinária , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Transcrição Reversa , Sorogrupo
J Toxicol Sci ; 34 Suppl 1: SP83-9, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19265293


The main focus of this study was to determine the optimal administration period concerning the toxic effects on ovarian morphological changes in the repeated dose toxicity study. In order to assess the morphological and functional changes induced in the ovary by cyclophosphamide (CP), the compound was administrated to female rats at dose levels of 0, 5, 10 and 20 mg/kg for the repeated dose toxicity study for 2 or 4 weeks, and at 0, 5, 10, and 20 mg/kg for the female fertility study from 2 weeks prior to mating to Day 7 of pregnancy. In the repeated dose toxicity study, increases in large sized atretic follicles, atrophy of corpora lutea were observed in the 20 mg/kg group in the 4-week study by the histopathological examination of the ovaries. There were no drug-related changes in the ovary in the 2-week study. In the female fertility study, the numbers of implantation were slightly decreased and the corpora lutea of pregnancy was not observed in the 20 mg/kg group. The dose-dependent increase in the incidence of post-implantation loss was observed, and no abnormalities were observed in the estrus cycle and mating in all treated groups. From these findings, the histopathological changes in the ovary are important endpoints for the evaluation of drug-induced ovarian damage as well as caesarean section. In conclusion, a 4-week administration period is sufficient to detect the ovarian toxicity of CP in the repeated dose toxicity study.

Antineoplásicos Alquilantes/toxicidade , Ciclofosfamida/toxicidade , Fertilidade/efeitos dos fármacos , Ovário/efeitos dos fármacos , Testes de Toxicidade/métodos , Animais , Antineoplásicos Alquilantes/administração & dosagem , Peso Corporal/efeitos dos fármacos , Ciclofosfamida/administração & dosagem , Esquema de Medicação , Ingestão de Alimentos/efeitos dos fármacos , Implantação do Embrião/efeitos dos fármacos , Perda do Embrião/induzido quimicamente , Embrião de Mamíferos/efeitos dos fármacos , Ciclo Estral/efeitos dos fármacos , Feminino , Injeções Intraperitoneais , Japão , Masculino , Tamanho do Órgão/efeitos dos fármacos , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/metabolismo , Folículo Ovariano/patologia , Ovário/patologia , Gravidez , Parcerias Público-Privadas , Ratos , Ratos Sprague-Dawley , Sociedades Científicas , Útero/efeitos dos fármacos , Útero/patologia , Vagina/efeitos dos fármacos , Vagina/patologia