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1.
Schweiz Arch Tierheilkd ; 161(10): 619-625, 2019 Oct.
Artigo em Alemão | MEDLINE | ID: mdl-31586924

RESUMO

INTRODUCTION: In most cases, bovine recumbency (downer cow syndrome) is a complication of periparturient hypocalcemia, but may also be caused by other metabolic disorders, trauma, toxic mastitis or metritis. An important treatment measurement in the recumbent cattle is to bring the cow to its feet, in order to avoid quickly occurring secondary damage. Various lifting systems are on the market, such as a cow lift with a supporting harness or hip clamps. Such lifting systems are easy to use and relatively inexpensive. However, these techniques can cause secondary injuries and ischemic pressure damage. An alternative method is the use of a specially designed flotation tank. The buoyancy of the water gently lifts the cattle avoiding secondary, neuromuscular lesions. The success rate in the therapy of recumbent cattle can be significantly improved by the correct and early use of suitable lifting techniques. In this review, the most common lifting techniques for recumbent cattle are summarized.

2.
Anim Genet ; 50(5): 423-429, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31294880

RESUMO

A specific white spotting phenotype, termed finching or line-backed spotting, is known for all Pinzgauer cattle and occurs occasionally in Tux-Zillertaler cattle, two Austrian breeds. The so-called Pinzgauer spotting is inherited as an autosomal incompletely dominant trait. A genome-wide association study using 27 white spotted and 16 solid-coloured Tux-Zillertaler cattle, based on 777k SNP data, revealed a strong signal on chromosome 6 at the KIT locus. Haplotype analyses defined a critical interval of 122 kb downstream of the KIT coding region. Whole-genome sequencing of a Pinzgauer cattle and comparison to 338 control genomes revealed a complex structural variant consisting of a 9.4-kb deletion and an inversely inserted duplication of 1.5 kb fused to a 310-kb duplicated segment from chromosome 4. A diagnostic PCR was developed for straightforward genotyping of carriers for this structural variant (KITPINZ ) and confirmed that the variant allele was present in all Pinzgauer and most of the white spotted Tux-Zillertaler cattle. In addition, we detected the variant in all Slovenian Cika, British Gloucester and Spanish Berrenda en negro cattle with similar spotting patterns. Interestingly, the KITPINZ variant occurs in some white spotted animals of the Swiss breeds Evolèner and Eringer. The introgression of the KITPINZ variant confirms admixture and the reported historical relationship of these short-headed breeds with Austrian Tux-Zillertaler and suggests a mutation event, occurring before breed formation.


Assuntos
Bovinos/genética , Cromossomos de Mamíferos , Pigmentação , Proteínas Proto-Oncogênicas c-kit/genética , Animais , Bovinos/classificação , Duplicação Cromossômica , Estudo de Associação Genômica Ampla , Variação Estrutural do Genoma , Polimorfismo de Nucleotídeo Único
3.
Anim Reprod Sci ; 181: 130-140, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28431863

RESUMO

This study aimed to investigate the in vitro contractility of the myometrium and its relationship to the blood concentrations of estradiol-17ß (E2ß), progesterone (P4), 15-keto-13,14-dihydro-PGF2α (PGFM) and ionised calcium (Ca2+) prior to tissue harvest in 12 healthy Holstein-Friesian cows in late pregnancy. Three circular (CM) and 3 longitudinal myometrial (LM) strips were dissected during a caesarean section and mounted in an organ bath containing modified Krebs solution (KS). The spontaneous contractility was recorded during five 30-min time periods (T1 to T5), after which the strips were exposed to increasing concentrations of oxytocin (OT; 10-10-10-7M), a natural PGF2α-analogue (PGF; 10-7-10-4M) and KS (Cont) for four 30-min time periods (T6 to T9). The variables area under the curve (AUC), mean (MA) and maximal amplitude (maxA) were calculated for each T. The blood P4, E2ß, Ca2+ and PGFM values averaged 4.0±1.7ng/mL, 482.3±63.7 pg/mL, 0.8±0.3 mmol/L and 125.3±63.7pg/mL. The LM strips had greater AUC, MA, and maxA than CM, and OT caused greater AUC and MA in both muscle layers than PGF or control treatment (OT>PGF>Cont). Estradiol-17ß correlated with AUC and MA of LM at T1 to T5 (r=0.69; P≤0.05). In conclusion, LM and CM strips have different contractile performance but show enhanced activity when stimulated with OT and less activity after PGF stimulation if compared with Cont. Blood concentrations of E2ß may be useful as an indicator of uterine contractile performance in late pregnant cattle.


Assuntos
Bovinos/fisiologia , Miométrio/fisiologia , Prenhez , Contração Uterina/fisiologia , Animais , Cálcio/sangue , Dinoprosta/análogos & derivados , Dinoprosta/sangue , Estradiol/sangue , Feminino , Gravidez , Prenhez/fisiologia , Progesterona/sangue
5.
Schweiz Arch Tierheilkd ; 157(9): 497-502, 2015 Sep.
Artigo em Alemão | MEDLINE | ID: mdl-26757590

RESUMO

The aim of this study was to obtain the diagnostic and therapeutic approach among Swiss practitioners in cows with retained fetal membranes (RFM) (part 1). All members of the Association for ruminant health were contacted per email via the newsletter. The survey was completed by 128 veterinarians, partially responded by 140 veterinarians. The manual removal of the fetal membranes is practiced by 129 of the responding veterinarians. Cows with/without fever are treated usually with intrauterine antibiotics. Cows with RFM with/without fever are most commonly treated parenterally with tetracycline or penicillin. The use of cephalosporins and quinolones in cows with fever is more common than in cows without fever. With the present results of the survey veterinarians should critically question the supposed benefits of the manual removal of the placenta and the use of antibiotics in cows with RFM.


Assuntos
Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/terapia , Placenta Retida/veterinária , Transtornos Puerperais/veterinária , Animais , Bovinos , Endometrite/diagnóstico , Endometrite/terapia , Endometrite/veterinária , Feminino , Sistemas On-Line , Placenta Retida/diagnóstico , Placenta Retida/terapia , Gravidez , Transtornos Puerperais/diagnóstico , Transtornos Puerperais/terapia , Inquéritos e Questionários , Suíça
6.
Schweiz Arch Tierheilkd ; 157(9): 503-12, 2015 Sep.
Artigo em Alemão | MEDLINE | ID: mdl-26757591

RESUMO

The aim of the study was to obtain the diagnostic and therapeutic approach among Swiss practitioners in cows with puerperal metritis and clinical endometritis (part 2). All members of the Association for ruminant health were contacted per email via the newsletter. The survey was completed by 128 veterinarians, partially responded by 140 veterinarians. The following main symptoms of puerperal metritis were stated by the practitioners: purulent vaginal discharge, fever and reduced appetite. A vaginal and rectal examination was performed to diagnose the disease. Usually, an intrauterine treatment with tetracycline or cefapirin was done. Parenteral administration of tetracycline or penicillin was often combined with PGF(2α), NSAIDS or cortisone. Clinical endometritis was also diagnosed by vaginal and rectal examination and the main symptom indicated was purulent vaginal discharge. The therapy consisted of the administration of PGF(2α), uterine infusions predominantly with cefapirin, and rarely with parenteral administration of antibiotics. Further diagnostic tools were not used and normally cows were not rechecked. The success of the therapy of puerperal metritis and clinical endometritis was judged to be satisfactory to excellent.


Assuntos
Anti-Infecciosos/administração & dosagem , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/terapia , Endometrite/veterinária , Transtornos Puerperais/veterinária , Administração Intravaginal , Animais , Bovinos , Endometrite/diagnóstico , Endometrite/terapia , Feminino , Sistemas On-Line , Transtornos Puerperais/diagnóstico , Transtornos Puerperais/terapia , Inquéritos e Questionários , Suíça
7.
Schweiz Arch Tierheilkd ; 157(6): 331-7, 2015 Jun.
Artigo em Alemão | MEDLINE | ID: mdl-26753348

RESUMO

Eringer cows are often slaughtered due to fertility problems which result from inflammatory and degenerative changes of the uterus or hormonal imbalances. Twenty-one genital tracts from Eringer cows suffering from fertility problems were collected in the abattoir. The purpose of the study was the macroscopic evaluation of the ovaries and the uterus followed by a histological and microbiological analysis of the uterus. Data from inseminations and calvings were provided by the Eringer breeding association and through the internet portal www.agate.ch. Median age of the cows was 6.9 years, number of calves per cow was 2.5 and median period between last calving and slaughter was 1.5 years. In 13 from 21 of the urogenital tracts examined, macroscopic abnormalities of the ovaries and/or histologic or microbiologic findings in the uterus could explain fertility-associated slaughter.


Assuntos
Doenças dos Bovinos/patologia , Infertilidade/veterinária , Ovário/patologia , Útero/patologia , Matadouros , Animais , Bactérias/isolamento & purificação , Bovinos , Doenças dos Bovinos/microbiologia , Feminino , Infertilidade/microbiologia , Infertilidade/patologia , Ovário/microbiologia , Útero/microbiologia
8.
J Dairy Sci ; 97(4): 2193-203, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24565325

RESUMO

Suppression of cyclic activity in cattle is often desired in alpine farming and for feedlot cattle not intended for breeding. A cattle-specific anti-GnRH vaccination (Bopriva, Zoetis Australia Ltd., West Ryde, Australia) is approved for use in heifers and bulls in New Zealand, Australia, Mexico, Brazil, Argentina, Turkey, and Peru. Eleven healthy, cyclic Swiss Fleckvieh cows were included in the study and vaccinated twice with Bopriva 4wk apart. Injection site, rectal body temperature, and heart and respiratory rates were recorded before and 3d following each vaccination. Blood samples were taken weekly for progesterone and estrogen analysis and to determine GnRH antibody titer. Ovaries were examined weekly, using ultrasound to count the number of follicles and identify the presence of a corpus luteum. Thirty weeks after the first vaccination, the cows were subjected to a controlled internal drug-releasing device-based Select-Synch treatment. The GnRH antibody titers increased after the second vaccination and peaked 2wk later. Estrogen levels were not influenced by vaccination, and progesterone level decreased in 7 of 11 cows up to 3wk after the second vaccination and remained low for 10 to 15wk following the second vaccination. The number of class I follicles (diameter ≤5mm) was not influenced by vaccination, whereas the number of class II follicles (diameter 6-9mm) decreased between 7 and 16wk after the first vaccination. Class III follicles (diameter >9mm) were totally absent during this period in most cows. The median period until recurrence of class III follicles was 78d from the day of the second vaccination (95% confidence interval: 60-92d). After vaccination, all cows showed swelling and pain at the injection site, and these reactions subsided within 2wk. Body temperature and heart and respiratory rates increased after the first and second vaccinations and returned to normal values within 2d of each vaccination. The cows in our study were not observed to display estrus behavior until 30wk after the first vaccination. Therefore, a Select-Synch protocol was initiated at that time. Ten cows became pregnant after the first insemination (the remaining cow was reinseminated once until confirmed pregnancy). Bopriva induced a reliable and reversible suppression of reproductive cyclicity for more than 2mo. The best practical predictor for the length of the anestrus period was the absence of class III follicles.


Assuntos
Anestro/efeitos dos fármacos , Bovinos/fisiologia , Estrogênios/sangue , Hormônio Liberador de Gonadotropina/farmacologia , Progesterona/sangue , Vacinação/veterinária , Animais , Anticorpos/sangue , Bovinos/imunologia , Feminino , Gravidez
10.
Schweiz Arch Tierheilkd ; 153(1): 7-13, 2011 Jan.
Artigo em Alemão | MEDLINE | ID: mdl-21210367

RESUMO

Herens cows have been treated at the Clinic for Ruminants, University of Berne, more frequently for fertility problems than other breeds. The aim of the study was to overview the reproductive performance of the Herens breed by analyzing data sets of the Herens Breeding Book and of the Animal Traffic Database of Switzerland. In addition, a questionnaire was sent to the breeders concerning aspects of management and care to identify a possible influence on the reproductive performance of the animals. Based on 4988 lactations starting in 2003, an average interval of calving to first insemination of 86 days a calving to conception interval of 146 days and an inter calving period of 431 days could be calculated. Conception rate resulted in 39.1%, the fertility index was 1.87 and 6.5% of all cows were culled because of fertility problems. Half of the breeders owned 4 or less cows. The most important reason for keeping Herens cows was cow fighting. Traditional alpine pasturing and cow fight rules resulted in a seasonal calving with 80% of the births taking place between October and December. The calving month and seasonal calving were the most important reasons for a prolonged calving to conception interval.


Assuntos
Cruzamento/estatística & dados numéricos , Bovinos/fisiologia , Reprodução/fisiologia , Criação de Animais Domésticos/métodos , Animais , Coeficiente de Natalidade , Bovinos/classificação , Feminino , Lactação/fisiologia , Gravidez , Taxa de Gravidez , Estações do Ano , Inquéritos e Questionários , Suíça
11.
Theriogenology ; 70(6): 880-6, 2008 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-18585773

RESUMO

The bovine myometrium exerts different contractility patterns in dependency of the reproductive state. However, little is known about the contractile behaviour of the uterus in the cow regarding topography. Differences in the in vitro contractile activity of two regions of the bovine uterus were assessed using an organ bath model. Myometrial specimens from the larger horn: near corpus (nc) and near tip (nt) from healthy cows in oestrus (n=11) or dioestrus (n=6) were collected after slaughter. Two strips were prepared from each region corresponding to the circular and the longitudinal muscle layers, respectively. Spontaneous contractility of these strips was recorded in an organ bath. For analysis, the 2.5 h recordings were divided into five periods of 30 min each. The variables area under curve (AUC) and maximal (Amax) and minimal amplitude (Amin) were calculated separately for periods, and the results analysed with a non-parametric model regarding the influence of cycle phase (estrus vs. diestrus), region (nc vs. nt) and muscle layer (circular vs. longitudinal). The region had a influence on AUC (p<0.05) with higher values for nt samples, while Amin was influenced by region (nt>nc) and muscle layer (longitudinal>circular) (p<0.05). By using the same model in subgroups of region, differences among periods for AUC and A(max) caused by increasing values over time, and an interaction of layerxtime for Amin were evident for nt samples (p<0.05). For nc samples, however, interactions of cyclexlayerxtime (AUC) and layerxtime (Amax, Amin) revealed to be significant (p<0.05). The relevance of the results for further research regarding uterine physiology is discussed.


Assuntos
Ciclo Estral/fisiologia , Miométrio/fisiologia , Contração Uterina/fisiologia , Útero/anatomia & histologia , Animais , Bovinos , Células Cultivadas , Feminino , Músculo Liso/citologia , Músculo Liso/fisiologia , Miométrio/anatomia & histologia , Técnicas de Cultura de Órgãos , Fatores de Tempo , Útero/citologia , Útero/fisiologia
12.
Vet Q ; 29(3): 112-6, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17970288

RESUMO

Pollakisuria in adult goats can be caused by diseases of the urinary tract and by distension of parts of the genital tract leading to irritation of the bladder. Hydrometra is the most common cause of uterine distension in goats and usually can be resolved by prostaglandin injections. But other pathologies of the uterus can generate a similar syndrome. A dwarf goat was presented at the clinic with a history of chronic pollakisuria and tenesm. An initial ultrasonographic examination of the abdomen led to the suspicion of hydrometra, but treatment with injections of prostaglandin were not successful. Blood samples revealed low progesterone and high oestrogen values. A laparotomy was performed and an enlarged uterus with 1.5 L of mucous content and cystic ovaries were found and partially removed. A single solid leiomyoma was diagnosed histologically in the uterine wall. Two months later the goat's condition had deteriorated and therefore she was euthanized. Necropsy and pathohistological examination revealed the presence of a metastasized adenocarcinoma of the uterus. In this case, the pollakisuria provoqued by distension of the uterus was not caused by hydrometra, but by neoplasia. The syndrome and the pathogenesis of the adenocarcinoma in consideration of the hormonal status of the patient is discussed.


Assuntos
Adenocarcinoma/veterinária , Doenças das Cabras/diagnóstico , Leiomioma/veterinária , Poliúria/veterinária , Neoplasias Uterinas/veterinária , Adenocarcinoma/complicações , Adenocarcinoma/diagnóstico , Animais , Diagnóstico Diferencial , Feminino , Doenças das Cabras/patologia , Cabras , Leiomioma/complicações , Leiomioma/diagnóstico , Poliúria/diagnóstico , Poliúria/etiologia , Neoplasias Uterinas/complicações , Neoplasias Uterinas/diagnóstico
13.
Vet Rec ; 161(12): 414-7, 2007 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-17890770

RESUMO

A double-blinded, randomised, placebo-controlled field study of the influence of prostaglandin E2 (PGE2) on cattle at parturition was carried out. The extent of cervical opening and the intensity of labour were scored before administration of the compound and 10 minutes later; routine birth assistance was then continued by the veterinarian. Successful birth occurred more quickly in the cows treated with PGE2. The extent of cervical opening before the administration of the drug had a significant effect on the time to delivery, but the intensity of labour and a concomitant infusion of calcium did not have significant effects on this period. The less open the cervix before administration of the drug, the more the duration of parturition differed between the two groups, with the placebo group taking longer. A telephone follow-up inquiry found no significant differences between the cows postpartum; there were cases of mastitis and hypocalcaemia in both groups. The incidence of retained fetal membranes and the mortality of the calves were higher in the placebo group, but in neither case was the difference significant.


Assuntos
Colo do Útero/efeitos dos fármacos , Dinoprostona/uso terapêutico , Trabalho de Parto Induzido/veterinária , Ocitócicos/uso terapêutico , Animais , Bovinos , Dinoprostona/administração & dosagem , Método Duplo-Cego , Feminino , Injeções Intravenosas , Ocitócicos/administração & dosagem , Gravidez , Suíça , Resultado do Tratamento
14.
Schweiz Arch Tierheilkd ; 147(10): 445-52, 2005 Oct.
Artigo em Alemão | MEDLINE | ID: mdl-16259410

RESUMO

In spring 2005, the outbreak of contagious abortion caused by Salmonella Abortusovis in 6 sheep flocks in Switzerland led to considerable economic losses. The Swiss small ruminant health service (BGK) evaluated this case. The aim was to identify the source of the epidemic in order to avoid further spread of infection and to evaluate the possibility of using vaccination. Moreover, a strategy for prevention of future outbreaks was developed. This article aims to increase disease awareness of food animal practitioners for Salmonella Abortusovis abortion in sheep.


Assuntos
Aborto Animal/microbiologia , Surtos de Doenças/veterinária , Salmonelose Animal/epidemiologia , Doenças dos Ovinos/epidemiologia , Animais , Feminino , Masculino , Gravidez , Complicações Infecciosas na Gravidez/microbiologia , Complicações Infecciosas na Gravidez/veterinária , Salmonelose Animal/complicações , Ovinos , Doenças dos Ovinos/microbiologia , Suíça/epidemiologia
15.
J Vet Med A Physiol Pathol Clin Med ; 51(9-10): 385-93, 2004 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-15610478

RESUMO

Serotoninergic and adrenergic receptors (5-HTR and AR) are involved in the regulation of uterine contractility. The objective of this study was to compare mRNA levels of 5-HTR(1A), 5-HTR(1B), 5-HTR(1D), 5-HTR(1F), 5-HTR(2A), 5-HTR(2B), 5-HTR(2C), 5-HTR(4) and alpha(1A), alpha(1B), alpha(1D), alpha(2AD), alpha(2B), alpha(2C), and beta(1), beta(2), beta(3)-AR in oestrus and dioestrus, and at three uterine locations (tip, middle and base) using quantitative real-time polymerase chain reaction. Uterine specimens consisting of endometrium and myometrium including vessels and serosa were collected from cows in oestrus (n = 10) and dioestrus (n = 15) respectively. Levels of 5-HTR and AR mRNA were expressed relative to the geometric mean of ribosomal RNA (18S), ubiquitin and glyceraldehyde phosphate dehydrogenase by the mean values of geNorm algorithm. 5-HTR(1A), 5-HTR(2C) and beta(3)-AR mRNA could not be detected in uterine tissues. The mRNA levels of 5-HTR(1F) and 5-HTR(2B) were lower (P < 0.05), but of 5-HTR(4) were higher (P < 0.05) in oestrus than in dioestrus. The mRNA levels of alpha(1A)-AR, alpha(2AD)-AR, alpha(2B)-AR were lower (P < 0.05), but of alpha(2C)-AR and beta(2)-AR were higher (P < 0.05) in oestrus than dioestrus. The mRNA levels of 5-HTR(1B) and 5-HTR(1D) (oestrus) and of alpha(2AD)-AR (dioestrus) differed among uterine locations (base > middle > tip; P < 0.05). The mRNA expression of 5-HTR and AR (subtypes) in bovine uterus was associated with cycle activity and varied according to uterine location. Additional studies on protein level will be carried out in order to elucidate the role of these receptor families on uterine contractility, which may then help to clarify clinical relevance.


Assuntos
Bovinos/fisiologia , Diestro/fisiologia , Estro/fisiologia , RNA Mensageiro/metabolismo , Útero/metabolismo , Animais , Feminino , Expressão Gênica , RNA Mensageiro/análise , Receptores Adrenérgicos/genética , Receptores Adrenérgicos/metabolismo , Receptores de Serotonina/genética , Receptores de Serotonina/metabolismo
16.
Schweiz Arch Tierheilkd ; 146(11): 515-21, 2004 Nov.
Artigo em Alemão | MEDLINE | ID: mdl-15581286

RESUMO

The purpose of this study was to describe the findings and therapy in 4 cows and 1 heifer with mummified fetus. All animals were admitted at the clinic after several unsuccessful therapies with prostaglandin F2alpha and local uterine infusions. All animals were in good condition. In case 1, diagnosis of mummified fetus could not be confirmed after manual rectal palpation and ultrasonography whereas cases 2, 4, 5 all had mummified fetus. In case 3, the fetus was in maceration. Initial therapy consisted of administration of prostaglandin F2alpha and prostaglandin E2 followed by repeated administration of prostaglandin E2. Mummies (length from apex to rump 13-32 cm) could be taken out within 3 to 6 days per vias naturales in cases 2, 4, 5 and in case 3, bones (maximal length 4 cm) could be unhinged. The structure in the uterus of case 1 could not be mobilised and was consequently removed under sight control using colpotomy followed by hysterotomy. Animals 2, 3, 4 and 5 were pregnant on the occasion of telephone inquiry. On the basis of our results, we recommend the conservative medical therapy with PGE2 for cases of mummified fetus. Colpotomy and hysterotomy are reserved as therapy feasible if the use of prostaglandin E2 is not successful.


Assuntos
Aborto Animal , Bovinos/embriologia , Morte Fetal/veterinária , Feto/patologia , Aborto Animal/induzido quimicamente , Aborto Animal/cirurgia , Animais , Bovinos/cirurgia , Colpotomia/métodos , Colpotomia/veterinária , Dinoprosta/administração & dosagem , Dinoprostona/administração & dosagem , Feminino , Morte Fetal/cirurgia , Feto/anatomia & histologia , Histerotomia/métodos , Histerotomia/veterinária , Gravidez
17.
Dtsch Tierarztl Wochenschr ; 111(1): 28-35, 2004 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-14983752

RESUMO

The effects of endotoxin (ET) on spontaneous contractility and of carbachol- and alpha-methyl-5-hydroxytryptamine-(alpha-M-5-HT; 5-HT2 receptor agonist) induced contractions of smooth muscle preparations from the bovine abomasal antrum were investigated in vitro. Preparations from the abomasal antrum of freshly slaughtered healthy dairy cows were cut parallel to the longitudinal and circular fibres, suspended in isolated organ baths, and contractility was recorded and analyzed, using digitalized data. The traits maximum amplitude, time till maximum amplitude, frequency, basal tone, and area under curve were calculated. The contractile effect of Carbachol (CH) was concentration dependent. Repeated administration of CH (3.75 x 10(-6) M), each time interrupted by flushing of the organ baths, did not reveal any significant effect on contractility traits of CH-induced contractions. Endotoxin (10 micrograms/ml; lipopolysaccharide from E. coli, O26:B6) significantly reduced some of the spontaneously occurring contractility traits and of carbachol-(3.75 x 10(-6) M) and alpha-M-5-HT-induced (2.14 x 10(-5) M) contractions. The effects of higher and lower concentrations of ET occurred less consistently. The inhibitory effect of endotoxin was more pronounced after 6 hours as compared to 2 hours of incubation. The results of the present study (i) support the hypothesis of a possible role of endotoxin in reducing motility of the abomasum during the development of spontaneous abomasal displacement in dairy cows, and (ii) may serve as the basis for the development of an in vitro model of abomasal displacement with endotoxemia for future studies on the effect of motility modulating drugs.


Assuntos
Abomaso/efeitos dos fármacos , Bovinos/fisiologia , Endotoxinas/farmacologia , Contração Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Abomaso/anormalidades , Abomaso/fisiologia , Animais , Área Sob a Curva , Carbacol/farmacologia , Agonistas Colinérgicos/farmacologia , Relação Dose-Resposta a Droga , Feminino , Técnicas In Vitro , Distribuição Aleatória , Serotonina/análogos & derivados , Serotonina/farmacologia , Agonistas do Receptor de Serotonina/farmacologia
18.
Anim Reprod Sci ; 79(1-2): 17-32, 2003 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-12853176

RESUMO

Prostaglandin F(2alpha) is used in dairy herd management because of its luteolytic properties and for its direct effect on the myometrium in cows diagnosed with endometritis. Prostaglandin E(2) has a contractile effect on the bovine uterus. In human medicine, prostaglandin E(2) is routinely used to maintain labor and to ripen the cervix. We hypothesized, that a combination of prostaglandin F(2alpha) and prostaglandin E(2) would provoke a long-lasting increase in intrauterine pressure (IUP) and uterine motility as compared to either prostaglandin group. Intrauterine pressure was recorded during the diestrus of eight lactating dairy cows using a transcervically placed intraluminal pressure microtransducer. After recording of physiologic uterine motility for 30min, prostaglandins (DL-cloprostenol, PGE(2), PGE(2) in combination with D-cloprostenol) or placebo were administered, followed by a 2h recording period. Significant differences were found for the area under the curve, the mean amplitude and the intrauterine pressure, whereas the number of pressure waves did not differ significantly among treatments. Peak values for area under the curve and mean amplitude were found during the first 15min for the combination of PGE(2) and D-cloprostenol. During the last 15min of the recording session, area under the curve and mean amplitude were increased only for the combination of PGE(2) and D-cloprostenol as compared to placebo. Although PGF(2alpha) and PGE(2) provoke an increase in intrauterine pressure, only their combination guarantees a significant effect over a 2h recording period.


Assuntos
Bovinos/fisiologia , Cloprostenol/farmacologia , Diestro , Dinoprostona/farmacologia , Útero/efeitos dos fármacos , Útero/fisiologia , Animais , Cloprostenol/administração & dosagem , Dinoprostona/administração & dosagem , Dinoprostona/efeitos adversos , Feminino , Placebos , Pressão , Progesterona/sangue , Contração Uterina/efeitos dos fármacos
19.
Schweiz Arch Tierheilkd ; 145(6): 283-90, 2003 Jun.
Artigo em Alemão | MEDLINE | ID: mdl-12847787

RESUMO

Since September 2001, castration of male calves in Switzerland is not allowed without anesthesia. The use of rubber rings for this purpose is forbidden. It was the goal of this study to describe the effect of a non-steroidal antiinflammatory drug, administered additionally to sedation and local anesthesia, in clamp-castrated (Burdizzo) calves of 110 to 160 kg of body weight. Plasma fibrinogen concentration, white blood cell count, serum cortisol concentration, scrotal swelling, concentrate intake and weight gain were evaluated. A positive effect after administration of a NSAID was obvious for the serum cortisol concentration, the concentrate intake within the first 3 days after castration and scrotal swelling.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Bovinos/fisiologia , Ingestão de Alimentos/efeitos dos fármacos , Hidrocortisona/sangue , Orquiectomia/veterinária , Ganho de Peso/efeitos dos fármacos , Animais , Bovinos/sangue , Bovinos/cirurgia , Fibrinogênio/análise , Contagem de Leucócitos/veterinária , Masculino , Orquiectomia/métodos , Escroto/patologia , Testículo/patologia
20.
J Vet Med A Physiol Pathol Clin Med ; 50(9): 442-6, 2003 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-15109237

RESUMO

Cervical smooth muscle specimens were collected from slaughtered cows in oestrus and diestrus. Longitudinal and circular specimens were mounted in organ baths and after equilibration time and 2 g pre-load, their physiologic isometric contractility was recorded for 2.5 h. Area under the curve (AUC), maximal amplitude (Amax), mean amplitude (Amen) and minimal amplitude (Amin) were studied. Differences between cycle phases, between muscle layers and over the recorded time periods were statistically evaluated using linear mixed-effect models. In the cow, physiologic contractility of the cervix decreased significantly over time for all variables evaluated (time as covariate on a continuous scale). Furthermore, for Amax, there was a tendency for an influence of muscle layer (longitudinal > circular). Contractility was not influenced by cycle phases.


Assuntos
Bovinos/fisiologia , Colo do Útero/fisiologia , Diestro/fisiologia , Estro/fisiologia , Contração Uterina/fisiologia , Animais , Feminino , Técnicas In Vitro , Músculo Liso/fisiologia , Progesterona/sangue
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