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1.
J Dent ; : 103267, 2019 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-31866414

RESUMO

OBJECTIVE: To be fit-for-purpose, oral health-related quality of life instruments must possess a range of psychometric properties which had not been fully examined in the 16-item Short Form Child Perceptions Questionnaire for children aged 11 to 14 years (CPQ11-14 ISF-16). We used advanced statistical approaches to determine the CPQ's measurement accuracy, precision, invariance and dimensionality and analyzed whether age range could be extended from 8 to 15 years. METHODS: Fit to the Rasch model was examined in 6648 8-to-15-year-olds from Australia, New Zealand, Brunei, Cambodia, Hong Kong, Malaysia, Thailand, Germany, United Kingdom, Brazil and Mexico. RESULTS: In all but two items, the initial five answer options were reduced to three or four, to increase precision of the children's selection. Items 10 (Shy/embarrassed) and 11 (Concerned what others think) showed an 'extra' dependency between item scores beyond the relationship related to the underlying latent construct represented by the instrument, and so were deleted. Without these two items, the CPQ was unidimensional. The three oral symptoms items (4 Food stuck in teeth, 3 Bad breath and 1 Pain) were required for a sufficient person-item coverage. In three out of 14 items (21 %), Europe and South America showed regional differences in the patterns of how the answer options were selected. No differential item functioning was detected for age. CONCLUSION: Except for a few modifications, the present analysis supports the combination of items, the cross-cultural validity of the CPQ with 14 items and the extension of the age range from 8 to 15 years. CLINICAL SIGNIFICANCE: The valid, reliable, shortened and age-extended version of the CPQ resulting from this study should be used in routine care and clinical research. Less items and a wider age range increase its usability. Symptoms items are needed to precisely differentiate between children with higher and lower quality of life.

2.
J Oral Rehabil ; 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31841219

RESUMO

BACKGROUND: Prevalence values for symptoms and signs of temporomandibular disorders (TMD) in adolescents vary within the literature due to differences in set-ups of examination protocols. OBJECTIVES: The objective was to present prevalence values for symptoms and signs of TMD in German adolescents that were observed within a standardised examination. Prevalence values comparing gender or pubertal status should be investigated. METHODS: A cross-sectional sample of the LIFE Child Study aged 10 to 18 was examined. A questionnaire gathering anamnestic symptoms of TMD was completed, and an examination according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) was performed by trained dentists. Pubertal status according to Tanner stages was documented. Descriptive statistics, Mann-Whitney U and Wilcoxon signed-rank tests were performed. RESULTS: A total of 1.116 participants (mean age 12.9 years, 51.4% female) were consecutively recruited. Most prevalent anamnestic symptoms were headaches (55.7%) and temporomandibular joint (TMJ) sounds (17.6%). The major clinical sign was TMJ sounds (31.9%). Both the examination of muscles or TMJs showed that pain to palpation was statistically significantly higher than familiar pain (P < .001). Gender comparisons revealed higher prevalence values and an increase in TMD symptoms and signs during pubertal development in females. CONCLUSION: The prevalence of self-reported headaches, as well as anamnestic and clinical TMJ sounds, was high in German adolescents. Prevalence values for pain to palpation and familiar pain obtained relevant differences. Considering the DC/TMD, females are more affected by TMD signs and symptoms that increase during pubertal development, than male adolescents.

3.
J Oral Rehabil ; 46(11): 1023-1030, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31206205

RESUMO

OBJECTIVES: The aim of this cross-sectional study was to investigate whether sex hormones (testosterone, oestradiol, sex-hormone-binding globulin = SHBG) are associated with general joint laxity (GJL) and hypermobility or derangements of the temporomandibular joint (TMJ) in adolescents. METHODS: Within the LIFE Child study, 970 adolescents (10-18 years) were included. GJL was assessed using the Beighton test. Maximum mouth opening (MMO) and clinical clicking sounds as signs of disc displacement (DD) in the TMJ were assessed according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Serum levels of sex hormones were assessed using standardised laboratory analyses. RESULTS: Hypermobile joints were found in 54.9% (N = 532) of the sample; females were more affected than males (61.4% vs. 51.8%, P < 0.001). Using logistic regression analyses, the odds ratio (OR) for having >1 hypermobile joints increased to 1.15 (95% confidence interval [CI]: 1.04-1.27) in males and 1.09 (95% CI: 1.02-1.17) in females per 10 units of the SHBG serum level, compared to those without hypermobile joints-after controlling for the effect of age, adjusted BMI, pubertal development (Tanner scale), testosterone as well as oestradiol levels. Female subjects with >1 hypermobile joints showed a higher OR (1.89; 95% CI: 1.05-3.43) for having clinical clicking sounds in the TMJ and a 3.28 times higher OR (95% CI: 1.44-7.44) for MMO ≥ 55 mm. CONCLUSIONS: We observed age- and gender-independent associations of higher SHBG serum levels with GJL in adolescents. Moreover, hypermobile female adolescents show a more frequent hypermobility of the TMJ and clinical signs of DD.


Assuntos
Instabilidade Articular , Transtornos da Articulação Temporomandibular , Adolescente , Criança , Estudos Transversais , Feminino , Hormônios Esteroides Gonadais , Humanos , Masculino , Articulação Temporomandibular
4.
J Am Dent Assoc ; 150(5): 352-361.e7, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30922518

RESUMO

BACKGROUND: Oral health is related to major chronic diseases and contributes to general health. Oral health-related quality of life (OHRQoL) is, therefore, an essential part of well-being. Measurement of OHRQoL over the lifetime requires accurate and comparable outcome measures that are suitable for different age groups. The most frequently used instrument for children aged 11 through 14 years is the Child Perceptions Questionnaire (CPQ-11-14). The authors aimed to examine the psychometric properties of the CPQ-11-14 using modern statistical approaches. METHODS: The authors carried out a psychometric analysis of 2 population-representative data sets from Germany (N = 2,658) and a randomly selected smaller sample (n = 300) on the basis of the Rasch model. RESULTS: The authors detected a right-skewed distribution of the CPQ-11-14 total scores. The instrument items did not match the level of OHRQoL of the study participants. Moreover, a large number of questionnaire items (range, 51.4%-94.3%) had so-called disordered thresholds, meaning that the answer categories did not work as intended. Unidimensionality was achieved via splitting the CPQ-11-14 into 2 subscales, namely socioemotional and oral symptoms and functioning. CONCLUSIONS: Despite its classic validation, the CPQ-11-14 needs adaptions for meaningful clinical use. More items on the better end of the scale are needed to better differentiate between children with higher and lower OHRQoL. PRACTICAL IMPLICATIONS: The Rasch model addresses psychometric properties of questionnaires on an item-based level, which were not detected via classic approaches. The results reported by the authors are needed to generate a well-calibrated outcome measure for accurate clinical and public health assessment of OHRQoL.


Assuntos
Saúde Bucal , Qualidade de Vida , Adolescente , Criança , Alemanha , Humanos , Inquéritos e Questionários
5.
J Dent ; 83: 1-6, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30710652

RESUMO

OBJECTIVES: To assess the efficacy of 8% arginine containing prophylaxis products over 24 weeks in subjects with dentin hypersensitivity (DH). METHODS: 297 patients with established DH (Schiff score 2, 3) in three European study centers were randomly assigned to either 8% arginine and calcium carbonate in-office prophylaxis paste and the respective toothpaste (test group) or fluoride-free prophylaxis paste and sodium monoflourophosphate toothpaste as a negative control group. Air blast (Schiff Score) and tactile (Visual Analog Scale) sensitivity scores were assessed at baseline (BL_0), after single application of the prophylaxis paste (BL_1) and after 4, 8, and 24 weeks of continuous at-home use of the toothpaste. RESULTS: 273 subjects completed the study. Test and control group presented statistically significant percentage reductions (t-test, p < 0.05) in Schiff Score at BL_1 and at 24 weeks relative to BL_0 (difference in %; test group: -23.6, -44.9, control group: -8.8, -32.7). The pooled Schiff Score for the two evaluated teeth yielded a significantly greater alleviation of DH in the test group than in the control group at all evaluation appointments (Ancova, p < 0.05; difference in %: 15.3, 7.4, 10.6, 17.2). CONCLUSIONS: A significant relief of DH was demonstrated after application of the 8% arginine prophylaxis products over 24 weeks compared to a negative control. CLINICAL SIGNIFICANCE: Whilst DH is influencing patients' eating, drinking and tooth brushing habits as well as social life interactions it is important that clinicians are able to offer evidence based immediate and long-lasting treatment methods in order to manage their patients' discomfort.


Assuntos
Arginina/uso terapêutico , Dessensibilizantes Dentinários/uso terapêutico , Sensibilidade da Dentina/tratamento farmacológico , Carbonato de Cálcio/uso terapêutico , Humanos , Fosfatos , Cremes Dentais , Resultado do Tratamento
6.
J Investig Clin Dent ; 10(2): e12394, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30663274

RESUMO

AIM: The aim of the present study was to investigate possible influences of overweight (OW)/obesity on caries in primary dentition. Socioeconomic status (SES) and quality of oral hygiene were also considered. METHODS: In the present study, 1628 children between the ages of 6 months and 9 years were included. In addition to dental examinations regarding the decayed, missing, filled teeth (dmft) index in primary dentition and the evaluation of oral hygiene, the body mass index standard deviation score (BMI SDS) was determined, and SES was recorded. RESULTS: The presence of OW/obesity was associated with higher caries prevalence (P < 0.001). A lower SES and non-optimal oral hygiene were also associated with increased dmft. The Poisson regression also showed a significantly higher risk in the combination of a high SES and OW/obesity (ßlow-high  = 0.21, P = 0.01). The increase was evident in both good and poor oral hygiene. In contrast, there was no difference between the lower and middle social strata (ßlow-medium  = 0.03, P = 0.6). With increasing age, the BMI influence decreased (ßage: BMI SDS  = -0.06, P < 0.001). CONCLUSIONS: BMI, oral hygiene, and SES are important factors in caries prevalence. In the highest social class, however, increased body weight has an adverse effect regardless of oral hygiene.


Assuntos
Cárie Dentária , Higiene Bucal , Criança , Índice CPO , Humanos , Lactente , Obesidade , Sobrepeso , Prevalência , Classe Social , Dente Decíduo
7.
Clin Oral Investig ; 23(4): 1793-1802, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30187257

RESUMO

OBJECTIVES: The aim of this study was to investigate the associations between blood levels of stress-related hormones and early signs of periodontal disease in children and adolescents. MATERIALS AND METHODS: Within the LIFE (Leipzig research center for civilization diseases) Child study, 498 adolescents (10 to 18 years) were included. Early signs of periodontal inflammation were measured by probing depth (PD) at six index teeth (16, 11, 26, 36, 31, 46). Blood levels of stress-related hormones (cortisol, dehydroepiandosterone-sulfate [DHEA-S]) and, additionally interleukine-6 (IL-6) were measured. Socioeconomic status, oral hygiene, orthodontic appliances, and nutritional status, recorded by body-mass-index-standard-deviation-score (BMI-SDS), were considered as confounding factors. Additionally, in 98 participants, an oral chairside active matrix metalloproteinase-8 (aMMP-8) test was performed. Statistical tests are the Mann-Whitney U tests, chi-squared tests and multivariate logistic regression model. RESULTS: IL-6, BMI-SDS as well as positive aMMP-8 test result were significantly associated with maximum PD > 3 mm (p < 0.05). However, no statistically significant associations between stress-related hormones (cortisol and DHEA-S) and presence of maximum PD > 3 mm were found (p > 0.05). Higher DHEA-S and BMI were associated with positive aMMP-8 result, even after adjusting for age and gender (p = 0.027, padj = 0.026). CONCLUSION: The results reveal no associations between PD and stress-related hormones cortisol and DHEA-S. aMMP-8 test result might be associated with DHEA-S level. Nutritional status seems to influence periodontal disease in adolescents. CLINICAL RELEVANCE: DHEA-S and BMI-SDS show associations with early signs of periodontal disease in adolescents aged 10 to 18 years. This association should be confirmed by the investigation of high-risk groups.


Assuntos
Sulfato de Desidroepiandrosterona/sangue , Hidrocortisona/sangue , Doenças Periodontais/epidemiologia , Estresse Psicológico/sangue , Adolescente , Índice de Massa Corporal , Criança , Feminino , Humanos , Interleucina-6/sangue , Masculino , Metaloproteinase 8 da Matriz/sangue
8.
Gesundheitswesen ; 81(4): 347-350, 2019 Apr.
Artigo em Alemão | MEDLINE | ID: mdl-28315288

RESUMO

The aim of this study was to determine whether dental care accessibility in Germany from 2002 to 2009 was linked to socioeconomic status (SES) or household net income (HHN). Analysis was based upon a nation-wide cross-sectional survey of German adults from 18 to 79 years (mean 49.1 years; 55% females) which was conducted by the "Bertelsmann Gesundheitsmonitor" from 2002-2009. Patients in Germany visit the dentist 2.4 times per year independently of the SES. Patients with higher income paid per income group 34 € (95%- KI: 6 €-63 €) more for their denture. People from the middle class had 1.28 (95% CI: 1.02-1.22), and people from the upper class had 1.86 (95%-CI: 1.58-2.18) as much dental coinsurance coverage as people from the lower class. The ability to pay for denture and obtain dental insurance coverage rose with higher SES or HHN. The rise of additional payments for dental services leads to discrepancies in dental health care.


Assuntos
Assistência Odontológica , Acesso aos Serviços de Saúde , Renda , Classe Social , Adulto , Estudos Transversais , Feminino , Alemanha , Humanos , Masculino , Fatores Socioeconômicos
9.
Int J Paediatr Dent ; 28(6): 648-657, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30246468

RESUMO

AIM: The purpose of this study was to investigate the relationship between the severity of hypomineralised teeth, plaque accumulation, hypersensitivity, associated caries risk, and oral health-related quality of life limitations of affected children. MATERIALS AND METHODS: A total of 250 children (mean age 9.0 years) presenting with at least one hypomineralised molar or incisor were included in the study. The hypersensitivity of the teeth was evaluated using the Schiff Cold Air Sensitivity Scale and the Wong-Baker Faces Scale. Furthermore, the Decayed, Missing, and Filled Teeth Index was documented, the Quigley Hein Index was obtained, and personal questioning was used to document the patients' limitations with intake of food. These data were analysed using general linear models that control influencing factors such as age and sex. RESULTS: The Quigley Hein Index increased with the severity of the hypomineralised teeth (1.64 in the unaffected, 2.77 in the moderately, and 3.63 in the severely affected teeth). Problems with intake of food were only observed in subjects with severe hypomineralisation. There was no immediate effect on dental caries. CONCLUSION: Increased severity of tooth hypomineralisation leads to increased hypersensitivity, which resulted in inadequate oral hygiene among children and limitations to their daily life.


Assuntos
Cárie Dentária/epidemiologia , Higiene Bucal , Desmineralização do Dente/epidemiologia , Adolescente , Fatores Etários , Criança , Pré-Escolar , Estudos Transversais , Assistência Odontológica , Índice de Placa Dentária , Restauração Dentária Permanente , Sensibilidade da Dentina/epidemiologia , Ingestão de Alimentos , Feminino , Alemanha/epidemiologia , Humanos , Incisivo/patologia , Masculino , Dente Molar/patologia , Prevalência , Qualidade de Vida , Fatores Sexuais
10.
Clin Oral Investig ; 22(1): 449-460, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28578462

RESUMO

OBJECTIVES: The suitability of a chairside aMMP-8 test in determination of periodontal inflammation and caries in adolescents was assessed. Secondly, the influence of orthodontic treatment on aMMP-8 test result was analyzed. MATERIALS AND METHODS: Within the LIFE Child study, 434 adolescents (10 to 18 years) were included. Clinical dental examinations comprised caries experience (DMF/T-Index), signs of periodontal inflammation (probing pocket depth, PPD; community periodontal index of treatment needs; CPITN) at six index teeth and oral hygiene (OH). Information about orthodontic appliances (OA) and socioeconomic status (SES) were obtained by validated questionnaires. Test's sensitivity and specificity to detect periodontal inflammation and carious lesions were evaluated. The influence of OA on the test result was analyzed (multivariate model). RESULTS: No associations between age, gender, SES or OH, and test outcome were found (p > 0.05). Positive test results correlated to periodontal findings (CPITN, mean PPD; p < 0.001). However, for the detection of ≥ 1 site(s) with PPD ≥ 4 mm, the test's sensitivity and specificity were found to be 61 and 69%, respectively. Multivariate analysis revealed a higher probability for a positive test result in cases of fixed OA (odds ratio 5.02, 95% confidence interval 1.90-13.19). The test had no diagnostic value considering carious lesions. CONCLUSIONS: The chairside aMMP-8 test does not reliably identify adolescents with periodontal inflammation. Positive test results were more frequent in case of OA. CLINICAL RELEVANCE: The chairside aMMP-8 test is no appropriate tool to screen children and adolescents neither for periodontal inflammation nor for carious lesions.


Assuntos
Cárie Dentária/diagnóstico , Metaloproteinase 8 da Matriz/metabolismo , Doenças Periodontais/diagnóstico , Adolescente , Criança , Índice CPO , Cárie Dentária/enzimologia , Cárie Dentária/epidemiologia , Estudos Epidemiológicos , Feminino , Humanos , Masculino , Higiene Bucal , Ortodontia Corretiva , Doenças Periodontais/enzimologia , Doenças Periodontais/epidemiologia , Índice Periodontal , Sensibilidade e Especificidade , Fatores Socioeconômicos , Inquéritos e Questionários
11.
Qual Life Res ; 27(3): 775-782, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29063350

RESUMO

PURPOSE: Aim of this exploratory study was to investigate whether a retrospective assessment of oral health-related quality of life (OHRQoL) using the Oral Health Impact Profile (OHIP) is susceptible to bias such as implicit theory of change and cognitive dissonance. METHODS: In this prospective clinical study, a sample of 126 adult patients (age 17-83 years, 49% women) requiring prosthodontic treatment was consecutively recruited. The OHRQoL was assessed using the 49-item OHIP at baseline and at follow-up. Additionally, patients were asked at follow-up to retrospectively rate their oral health status at baseline (retrospective pretest or then-test) and the change in oral health status using a global transition question. Furthermore, patients' ratings of overall oral health and general health were used as validity criteria for the OHRQoL assessments. Response shift was calculated as the difference between the initial and retrospective baseline assessments. RESULTS: Baseline and retrospective pretest did not differ substantially in terms of internal consistency and convergent validity. Response shift was more pronounced when patients perceived a large change in OHRQoL during treatment. Retrospective pretests were more highly correlated with the baseline than with the follow-up assessment. CONCLUSION: Findings suggest that retrospective assessments of OHRQoL using the OHIP-49 are susceptible to bias. Cognitive dissonance is more likely to appear as a source of bias than implicit theory of change.


Assuntos
Saúde Bucal/normas , Qualidade de Vida/psicologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Viés , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Inquéritos e Questionários , Adulto Jovem
12.
Int J Colorectal Dis ; 33(2): 209-218, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29273882

RESUMO

PURPOSE: The oral microbiome has been related to numerous extra oral diseases. Recent studies detected a high abundance of oral bacteria in inflamed appendices in pediatric patients. To elucidate the role of oral bacteria in acute pediatric appendicitis, we studied the oral and appendiceal microbiome of affected children compared to healthy controls. METHODS: Between January and June 2015, 21 children undergoing appendectomy for acute appendicitis and 28 healthy controls were prospectively enrolled in the study. All individuals underwent thorough dental examination and laboratory for inflammatory parameters. Samples of inflamed appendices and the gingival sulcus were taken for 16S rDNA sequencing. RT-qPCR of Fusobacterium nucleatum, Peptostreptococcus stomatis, and Eikenella corrodens was performed and their viability was tested under acidic conditions to mimic gastric transfer. RESULTS: In phlegmonous appendices, Bacteroidetes and Porphyromonas were discovered as dominant phylum and genus. In sulcus samples, Firmicutes and Streptococcus were detected predominantly. P. stomatis, E. corrodens, and F. nucleatum were identified in each group. Viable amounts of P. stomatis were increased in sulci of children with acute appendicitis compared to sulci of healthy controls. In inflamed appendices, viable amounts of E. corrodens and F. nucleatum were decreased compared to sulci of children with appendicitis. Postprandial viability could be demonstrated for all tested bacteria. CONCLUSION: In children with acute appendicitis, we identified several oral bacterial pathogens. Based on postprandial viability of selected species, a viable migration from the oral cavity through the stomach to the appendix seems possible. Thus, the oral cavity could be a relevant reservoir for acute appendicitis.


Assuntos
Apendicite/microbiologia , Microbiota , Mucosa Bucal/microbiologia , Doença Aguda , Criança , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Viabilidade Microbiana , Microbiota/genética , RNA Ribossômico 16S/genética , Especificidade da Espécie
13.
J Orofac Orthop ; 79(1): 29-38, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29260241

RESUMO

BACKGROUND: Usually, morphological parameters of the teeth are recorded to help assess the indication for orthodontic treatment. It is assumed that significant deviations from average values compromise the quality of life. The aim of this study is to analyse the impact of overbite and overjet on oral health-related quality of life (OHRQoL) of children and adolescents. PATIENTS AND METHODS: A total of 748 subjects, aged 9.5-15.5 years, participated in the LIFE child project of the University of Leipzig, where they underwent a general medical and dental examination. Overbite and overjet were measured, and aberrations of the OHRQoL were recorded by the probands themselves, who completed the German version of the Child Perceptions Questionnaire (CPQ-G11-14). The OHRQoL is divided into four domains ("oral symptoms", "functional limitations", "emotional well-being" and "social well-being") and is analysed by means of a CPQ score depending on age, gender, socioeconomic status and orthodontic treatment. RESULTS: On average, the participants listed 10.5 (±13.1) problem issues on a CPQ scale ranging from 0 to 140. Subjects with current orthodontic treatment had a CPQ score about 2.5 (±2.4) higher than those without treatment. The aberrations were mainly observed in the domains "oral symptoms" and "functional limitations". Multiple linear regression showed that deviations of the overbite had only little influence on the OHRQoL, but deviations of an overjet-especially of >6 mm increased the CPQ summary score about 6 points. CONCLUSION: Children and adolescents with overjet deviations of >6 mm in comparison to the norm are associated with significant limitations of the OHRQoL. However, overbite deviations have only little influence.


Assuntos
Saúde Bucal , Sobremordida/complicações , Qualidade de Vida , Adolescente , Criança , Feminino , Alemanha , Humanos , Masculino , Ortodontia Corretiva/psicologia , Sobremordida/diagnóstico , Sobremordida/psicologia , Sobremordida/terapia , Qualidade de Vida/psicologia
14.
Eur J Epidemiol ; 32(2): 145-158, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28144813

RESUMO

The LIFE Child study is a large population-based longitudinal childhood cohort study conducted in the city of Leipzig, Germany. As a part of LIFE, a research project conducted at the Leipzig Research Center for Civilization Diseases, it aims to monitor healthy child development from birth to adulthood and to understand the development of lifestyle diseases such as obesity. The study consists of three interrelated cohorts; the birth cohort, the health cohort, and the obesity cohort. Depending on age and cohort, the comprehensive study program comprises different medical, psychological, and sociodemographic assessments as well as the collection of biological samples. Optimal data acquisition, process management, and data analysis are guaranteed by a professional team of physicians, certified study assistants, quality managers, scientists and statisticians. Due to the high popularity of the study, more than 3000 children have already participated until the end of 2015, and two-thirds of them participate continuously. The large quantity of acquired data allows LIFE Child to gain profound knowledge on the development of children growing up in the twenty-first century. This article reports the number of available and analyzable data and demonstrates the high relevance and potential of the study.


Assuntos
Desenvolvimento do Adolescente/fisiologia , Desenvolvimento Infantil/fisiologia , Estilo de Vida , Obesidade/epidemiologia , Adolescente , Adulto , Fatores Etários , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Alemanha/epidemiologia , Humanos , Lactente , Estudos Longitudinais , Masculino , Gravidez , Adulto Jovem
15.
J Biol Chem ; 291(23): 12195-207, 2016 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-27053108

RESUMO

A quality control system in the endoplasmic reticulum (ER) efficiently discriminates polypeptides that are in the process of productive folding from conformers that are trapped in an aberrant state. Only the latter are transported into the cytoplasm and degraded in a process termed ER-associated protein degradation (ERAD). In the ER, an enzymatic cascade generates a specific N-glycan structure of seven mannosyl and two N-acetylglucosamine residues (Man7GlcNAc2) on misfolded glycoproteins to facilitate their disposal. We show that a complex encompassing the yeast lectin-like protein Htm1 and the oxidoreductase Pdi1 converts Man8GlcNAc2 on glycoproteins into the Man7GlcNAc2 signal. In vitro the Htm1-Pdi1 complex processes both unfolded and native proteins albeit with a preference for the former. In vivo, elevated expression of HTM1 causes glycan trimming on misfolded and folded proteins, but only degradation of the non-native species is accelerated. Thus, modification with a Man7GlcNAc2 structure does not inevitably commit a protein for ER-associated protein degradation. The function of Htm1 in ERAD relies on its association with Pdi1, which appears to regulate the access to substrates. Our data support a model in which the balanced activities of Pdi1 and Htm1 are crucial determinants for the efficient removal of misfolded secretory glycoproteins.


Assuntos
Degradação Associada com o Retículo Endoplasmático , Glicoproteínas/metabolismo , Manosidases/metabolismo , Isomerases de Dissulfetos de Proteínas/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Retículo Endoplasmático/metabolismo , Glicoproteínas/química , Glicoproteínas/genética , Immunoblotting , Manosidases/química , Manosidases/genética , Complexos Multiproteicos/química , Complexos Multiproteicos/genética , Complexos Multiproteicos/metabolismo , Mutação , Polissacarídeos/química , Polissacarídeos/metabolismo , Ligação Proteica , Isomerases de Dissulfetos de Proteínas/química , Isomerases de Dissulfetos de Proteínas/genética , Dobramento de Proteína , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética
16.
J Orofac Orthop ; 77(1): 45-51, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26744208

RESUMO

OBJECTIVES: White spots are more common in patients with cleft lip and palate (CLP) than in the normal population. Whether these are due to the cleft itself or concomitant circumstances (e.g., surgical procedures, orthodontic treatments, systemic fluoridation, increased caries risk) remains unclear. This case-control study evaluated both their prevalence in CLP patients versus control subjects and associated risk factors. PATIENTS AND METHODS: A total of 73 CLP patients (average age 8.7 years, range 6-18 years, 42 % male) and a control group of 73 age- and gender-matched non-CLP patients were included. Enamel color changes, subsuming mineralization defects (DDE index), mild dental fluorosis (Dean's index), and initial caries (ICDAS score 2), were recorded. Caries index (dmf-t/DMF-T) scores were also recorded to distinguish between high or low caries risk as defined by the Deutsche Arbeitsgemeinschaft für Jugendzahnpflege criteria. Histories of systemic fluoridation, trauma to primary teeth, surgery, and orthodontic treatment were obtained using a questionnaire. Statistical analysis included t test, χ (2) test, and multivariable logistic regression. RESULTS: Enamel color changes were observed three times more often in the CLP group than in the control group (39.7 vs. 12.3 %; p < 0.001). Significantly more patients in the CLP group had a history of orthodontic treatment (38.4 vs. 15.1 %; p < 0.05). An increased risk for enamel color changes was associated with CLP itself [OR (odds ratio) 3.6; 95 % confidence interval (CI) 1.3-9.9] and table salt plus tablets combined for systemic fluoridation (OR 2.7, 95 % CI 1.1-6.9). No increased risks were identified for increased caries risk, history of primary-tooth trauma, or history of orthodontic treatment. CONCLUSION: The higher prevalence of enamel color changes in the CLP group (more than threefold compared to the control group) was not related to previous orthodontic treatments; however, systemic fluoridation (table salt and tablets) constituted a risk factor for the enamel color changes seen in the CLP patients.


Assuntos
Cárie Dentária/epidemiologia , Hipoplasia do Esmalte Dentário/epidemiologia , Esmalte Dentário/patologia , Descoloração de Dente/epidemiologia , Adolescente , Estudos de Casos e Controles , Criança , Fenda Labial , Fissura Palatina , Cor , Colorimetria/estatística & dados numéricos , Comorbidade , Cárie Dentária/diagnóstico , Cárie Dentária/patologia , Hipoplasia do Esmalte Dentário/diagnóstico , Hipoplasia do Esmalte Dentário/patologia , Alemanha/epidemiologia , Humanos , Masculino , Prevalência , Reprodutibilidade dos Testes , Fatores de Risco , Sensibilidade e Especificidade , Descoloração de Dente/diagnóstico , Descoloração de Dente/patologia , Adulto Jovem
17.
J Orofac Orthop ; 76(4): 305-17, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26123732

RESUMO

OBJECTIVES: Parafunctional habits such as clenching or grinding (bruxism) during daytime and at night are considered to have a great impact on the etiopathogenesis of temporomandibular disorders (TMD). However, the size of the effect and how daytime activities interact with nocturnal activities is not yet clear. The aim of this study was to assess the association of TMD pain with both awake and sleep bruxism in adults. MATERIALS AND METHODS: In this case-control study, data of a consecutive sample of 733 TMD patients (cases; mean age ± SD: 41.4 ± 16.3 years; 82% women) with at least one pain-related TMD diagnosis according to the German version of the Research Diagnostic Criteria for TMD (RDC/TMD) and of a community-based probability sample of 890 subjects (controls; mean age ± SD: 40.4 ± 11.8 years; 57% female) without TMD were evaluated. Clenching or grinding while awake and/or asleep was assessed with self-reports. Association of TMD pain with awake and sleep bruxism was analyzed using multiple logistic regression analyses and controlled for potential confounders. Odds ratios (OR) with corresponding 95% confidence intervals (CI) were calculated. RESULTS: While 11.2% of the controls reported clenching or grinding while awake, this proportion was significantly higher in TMD patients (33.9%; p < 0.001). Nocturnal clenching or grinding was reported by 23.5% of the controls and 49.4% of the TMD patients (p < 0.001). Risk for TMD pain did not differ substantially for the separate reports of awake (OR 1.7; CI 1.0-2.7) or sleep bruxism (OR 1.8; CI 1.4-2.4). However, risk for TMD pain substantially increased in cases of simultaneous presence of awake and sleep bruxism (OR 7.7; CI 5.4-11.1). CONCLUSION: When occurring separately, awake and sleep bruxism are significant risk factors for TMD pain. In case of simultaneous presence, the risk for TMD pain is even higher.


Assuntos
Artralgia/epidemiologia , Bruxismo/epidemiologia , Bruxismo do Sono/epidemiologia , Transtornos da Articulação Temporomandibular/epidemiologia , Adulto , Comorbidade , Feminino , Alemanha/epidemiologia , Humanos , Masculino , Medição da Dor/estatística & dados numéricos , Prevalência , Fatores de Risco , Distribuição por Sexo
18.
Clin Oral Investig ; 19(8): 1939-45, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25700701

RESUMO

OBJECTIVES: Questionnaires that measure oral health-related quality of life (OHRQoL) in children and adolescents have emerged in recent years as an important source of patient-reported outcomes. The aim of this study was to investigate potential effects of the method of administration (face-to-face interview, telephone interview, or self-administered questionnaire) in 11- to 14-year-old children and adolescents on OHRQoL information obtained using the Child Perceptions Questionnaire (CPQ). MATERIALS AND METHODS: OHRQoL was measured using the German version of the CPQ (CPQ-G11-14). The instrument was administered to 42 children and adolescents aged 11 to 14 years using the three different methods in a randomized order with an interval of 1 week between each administration. Test-retest reliability for the repeated CPQ-G11-14 assessments across the three methods of administration, internal consistency, and convergent validity were determined. RESULTS: The CPQ-G11-14 mean summary scores did not vary statistically significantly across the three administration methods (P = 0.274). Test-retest reliability was moderate to good (ICC 0.69-0.82), internal consistency was satisfactory (Cronbach's alpha 0.85-0.88), and CPQ-G11-14 mean summary scores were correlated in the expected direction with a global measure of self-reported oral health for all the three administration methods. CONCLUSION: In conclusion, the method of administration (face-to-face interview, telephone interview, or self-administered questionnaire) did not influence CPQ-G11-14 scores in 11- to 14-year-old children and adolescents to a significant extent. CLINICAL RELEVANCE: Investigators in German-speaking countries can choose between all three methods of administration to obtain valid and reliable OHRQoL information.


Assuntos
Saúde Bucal , Qualidade de Vida , Inquéritos e Questionários , Adolescente , Criança , Feminino , Humanos , Masculino
19.
J Prosthet Dent ; 112(3): 465-71, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24636759

RESUMO

STATEMENT OF PROBLEM: Tooth wear is an increasing problem in a society where people are living longer. PURPOSE: The purpose of this study was to assess the effect of age, sex, and location of teeth on the severity of tooth wear and to determine the prevalence of dentin exposure in the general population of Germany. MATERIAL AND METHODS: Tooth wear was measured in casts of both jaws of 836 persons with a 6-point (0-5) ordinal rating scale. Linear random-intercept regression models with the covariates of age, sex, jaw, and tooth group (with the participant as a grouping variable) were computed to determine the association of these covariates with tooth wear of a single tooth. RESULTS: The mean tooth wear score across all age groups, both sexes, and all teeth was 2.9 (standard deviation, 0.8), and the prevalence of teeth with exposed dentin was 23.4%. The participants' age was correlated with the mean tooth wear scores (r=0.51). The tooth wear level among women was on average 0.15 units lower than among men, and tooth wear was on average 0.59 units higher for anterior teeth than for posterior teeth. CONCLUSIONS: Increased tooth wear in anterior teeth may be due to the initially predominant guidance by anterior teeth, with age-related linear progress in tooth wear. Occlusal tooth wear scores and dentin exposure increase with age.


Assuntos
Desgaste dos Dentes/epidemiologia , Adulto , Fatores Etários , Dente Pré-Molar/patologia , Dente Canino/patologia , Dentina/patologia , Feminino , Alemanha/epidemiologia , Humanos , Incisivo/patologia , Masculino , Mandíbula/patologia , Maxila/patologia , Pessoa de Meia-Idade , Dente Molar/patologia , Prevalência , Fatores Sexuais , Coroa do Dente/patologia , Adulto Jovem
20.
Eur J Oral Sci ; 122(1): 70-7, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24563919

RESUMO

The literature presents conflicting findings on whether health-related quality of life (HRQoL) measures have sufficient evaluative properties to assess changes caused by dental interventions. The aim of our study was to compare sensitivity to change in HRQoL and OHRQoL in prosthodontic patients. In this prospective intervention study, a total of 165 consecutively recruited patients completed the Short Form-36 (SF-36) and the 49-item Oral Health Impact Profile (OHIP), as self-administered questionnaires, before prosthodontic treatment and 1 month after treatment was finished. Differences in SF-36 and OHIP scores between baseline and follow up were tested for statistical significance using paired t-tests. Effect sizes (Cohen's d) were calculated. Health-related quality of life improved during prosthodontic treatment, indicated by a slight, but statistically significant, increase in the SF-36 physical component (difference: 1.0 points), whereas perceived mental health did not change substantially (difference: −0.5 points). Improvement in OHRQoL (difference in OHIP sum score: −6.7 points) was statistically significant. Although the OHIP effect size (of 0.2) was considered as small, according to guidelines, it was greater than for the SF-36 component scores (physical: 0.1; mental: 0.1). Sensitivity to change in quality of life measures was greater for OHRQoL than for HRQoL, limiting the usefulness of HRQoL as an outcome measure in dentistry.


Assuntos
Dentaduras , Nível de Saúde , Saúde Bucal , Qualidade de Vida , Atividades Cotidianas , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Atitude Frente a Saúde , Coroas/psicologia , Prótese Total/psicologia , Prótese Parcial Fixa/psicologia , Prótese Parcial Removível/psicologia , Dentaduras/psicologia , Emoções , Feminino , Seguimentos , Humanos , Relações Interpessoais , Masculino , Saúde Mental , Pessoa de Meia-Idade , Dor/psicologia , Estudos Prospectivos , Sensibilidade e Especificidade , Adulto Jovem
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