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1.
Chemosphere ; 251: 126358, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32155493

RESUMO

Acidic substances, which produced during chlorinated volatile organic compounds, will corrode the commonly used packing materials, and then affect the removal performance of biofiltration. In this study, three biofilters with different filter bed structure were established to treat gaseous chlorobenzene. CaCO3 and 3D matrix material was added in filter bed as pH buffering material and filter bed supporting material, respectively. A comprehensive investigation of removal performance, biomass accumulation, microbial community, filter bed height, voidage, pressure drops, and specific surface area of the three biofilters was compared. The biofilter with CaCO3 and 3D matrix material addition presented stable removal performance and microbial community, and greater biomass density (209.9 kg biomass/m3 filter bed) and growth rate (0.033 d-1) were obtained by using logistic equation. After 200 days operation, the height, voidage, pressure drop, specific surface area of the filter bed consisted of perlite was 27.4 cm, 0.39, 32.8 Pa/m, 974,89 m2/m3, while those of the filter bed with CaCO3 addition was 28.2 cm, 0.43, 21.3 Pa/m, and 1021.03 m2/m3, and those of the filter bed with CaCO3 and 3D matrix material addition was 28.7 cm, 0.55, 17.4 Pa/m, and 1041.60 m2/m3. All the results verified the biofilter with CaCO3 and 3D matrix material addition is capable of sustaining the long-term performance of biofilters. CaCO3 could limit the changes of removal efficiency, microbial community and filter bed structure by buffering the pH variation. And 3D matrix material could maintain the filter bed structure by supporting the filter bed, regardless of the buffering effect.

2.
Sheng Wu Gong Cheng Xue Bao ; 36(2): 210-225, 2020 Feb 25.
Artigo em Chinês | MEDLINE | ID: mdl-32147994

RESUMO

Clostridioes difficile is a Gram-positive, spore-forming, obligate anaerobic bacterium, and the main cause of hospital-associated diarrhea. In recent years, with the presence of virulent strains (i.e., ribosome type 027), the prevalence and mortality events have increased. Thus, studies on physiological and biochemical characteristics, and pathogenic mechanisms of C. difficile have been performed. The development of efficient and stable genome-editing methods for C. difficile is urgent for the dissection of its physiological and pathogenic mechanism. For example, ClosTron technology plays a key role in study of the relationship between C. difficile toxins (Toxin A and Toxin B) and its pathogenicity. This article reviews the history, recent progress and future prospects of C. difficile genome-editing technologies.


Assuntos
Edição de Genes , Proteínas de Bactérias , Toxinas Bacterianas , Composição de Bases , Clostridium difficile , Enterotoxinas , Filogenia , RNA Ribossômico 16S , Análise de Sequência de DNA
3.
Zhongguo Gu Shang ; 33(1): 15-20, 2020 Jan 25.
Artigo em Chinês | MEDLINE | ID: mdl-32115919

RESUMO

OBJECTIVE: To analyze the effect of computer navigation assisted total knee arthroplasty on intraoperative hemorrhage and postoperative joint function recovery in patients with knee osteoarthritis. METHODS: From February 2015 to December 2017, 65 patients with knee osteoarthritis treated by traditional total knee arthroplasty were retrospectively analyzed as the control group and 65 patients with knee osteoarthritis treated by total knee arthroplasty under computer navigation as the experimental group. Before operation, all patients showed red swelling pain of knee, pain of going up and down stairs, and pain and discomfort of waist when sitting up and standing up. All patients were treated with total knee arthroplasty. The control group was treated with traditional total knee arthroplasty, and the experimental group was treated with total knee arthroplasty under the computer navigation system. The operation related conditions of the two groups were recorded and compared including the operation time and hospitalization time; the changes of hemoglobin and hematocrit of the two groups were detected and compared before and 5 days after the operation; the blood loss of the two groups and the induced flow at each time point calculated and compared after the operation, and the perioperative allogeneic blood transfusion rate and average blood transfusion volume of the patients were recorded; The joint function scale (KSS) was used to evaluate the recovery of knee joint function before the operation, 6 and 18 months after the operation respectively and to record the incidence of postoperative infection, lower extremity venous thrombosis and other complications. RESULTS: All the patients were successfully operated and the prognosis of the wound was good. All the patients were followed up for an average of 18 months. The operation time of the experimental group was longer than that of the control group, and the hospitalization time was shorter than that of the control group (P <0.05) ; the KSS score of the two groups at each time point after operation was higher than that before operation, but the increasing range of the test group was higher than that of the control group (P<0.05) ; there was no significant difference between the two groups in the incidence of complications (P>0.05) . CONCLUSION: Under the guidance of computer navigation, total knee arthroplasty can prolong the operation time compared with single total knee arthroplasty, but it is more conducive to reduce perioperative blood loss, reduce the rate of postoperative allogeneic blood transfusion, ideal recovery of joint function, less complications, safety and reliability.


Assuntos
Artroplastia do Joelho , Perda Sanguínea Cirúrgica , Humanos , Articulação do Joelho , Osteoartrite do Joelho , Recuperação de Função Fisiológica , Reprodutibilidade dos Testes , Estudos Retrospectivos , Resultado do Tratamento
4.
Mol Divers ; 2020 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-32130644

RESUMO

Piperine is the main active component of Piper longum L., which is also the main component of anti-sciatica Mongolian medicine Naru Sanwei pill. It has many pharmacological activities such as anti-inflammatory and immune regulation. This paper aims to preliminarily explore the potential mechanism of piperine in the treatment of sciatica through network pharmacology and molecular docking. TCMSP, ETCM database and literature mining were used to collect the active compounds of Piper longum L. Swiss TargetPrediction and SuperPred server were used to find the targets of compounds. At the same time, CTD database was used to collect the targets of sciatica. Then the above targets were compared and analyzed to select the targets of anti-sciatica in Piper longum L. The Go (gene ontology) annotation and KEGG pathway of the targets were enriched and analyzed by Metascape database platform. The molecular docking between the effective components and the targets was verified by Autodock. After that, the sciatica model of rats was established and treated with piperine. The expression level of inflammatory factors and proteins in the serum and tissues of rat sciatic nerve were detected by ELISA and Western blot. HE staining and immunohistochemistry were carried out on the sciatica tissues of rats. The results showed that Piper longum L. can regulate the development of sciatica and affect the expressions of PPARG and NF-kB1 through its active ingredient piperine, and there is endogenous interaction between PPARG and NF-kB1.

5.
Curr Microbiol ; 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32140834

RESUMO

Characterizing soil bacterial community is important to understand microbial distribution affected by environmental factors. Here, we investigated the bacterial communities distributed from different location on Nanshazhou island, South China Sea. We collected and compared soil bacterial communities from central island, intertidal island, and inshore island. Results showed no difference in the bacterial richness and diversity for the soils from the three different locations. However, weighted and unweighted UniFrac distances analysis revealed that the three soil samples were clearly separated from each other. Five bacterial phyla, including Proteobacteria, Bacteroidetes, Verrucomicrobia, Spirochaetes and Tenericutes were more abundant in the inshore island sample; while Deinococcus-Thermus was more abundant in the intertidal island sample; and the central island sample had more abundant Gemmatimonadetes and Planctomycetes. Bacterial structure showed significantly positive relationships with organic matter content, but it was significantly negatively correlated with sodium content. Furthermore, a network analysis based on Spearman correlation coefficients showed that there were similar numbers of positive and negative correlations within the bacterial community of Nanshazhou island. Our results revealed that the soil bacterial communities in the three sampling sites were easily affected by environmental fluctuations.

6.
Addict Behav ; 107: 106383, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32200196

RESUMO

Previous studies have reported well-documented findings that mobile phone addiction (MPA) is associated with negative emotion-related consequences; however, sporadic research has investigated the associations between MPA and cognitive outcomes related to daily cognitive functioning. Sleep duration, sleep quality, and trait self-regulation are thought to be linked to this association. The present study aimed to examine the mediating roles of sleep duration and quality and the moderating role of trait self-regulation between MPA and daily cognitive failures. A total of 1721 secondary school students were recruited to complete four self-reported questionnaires. The model results indicated that sleep quality (but not sleep duration) partially mediated the association between MPA and daily cognitive failures, and high levels of trait self-regulation could attenuate the potential impact of MPA on daily cognitive failures through sleep quality. Overall, these findings address the issue of how and when MPA is linked with cognitive performance in daily life, which can advance a better understanding of the negative consequences induced by MPA. Limitations and implications are discussed.

7.
Cell Res ; 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32203135

RESUMO

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

8.
BMC Cardiovasc Disord ; 20(1): 109, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32138662

RESUMO

BACKGROUND: Patients with coronary chronic total occlusion (CTO) require effective antiplatelet therapy after percutaneous coronary intervention (PCI). Ticagrelor has more pronounced platelet inhibition than clopidogrel. However, the most appropriate dose of ticagrelor in East Asian populations remains unclear. METHOD: We compared ticagrelor (180 mg loading dose, 90 mg twice daily thereafter and 120 mg loading dose, 60 mg twice daily thereafter) and clopidogrel (300 mg loading dose, 75 mg daily thereafter) for prevention of cardiovascular events in 525patients with CTO undergoing PCI. RESULTS: The rate of in-hospital major adverse cardiac and cerebral events (MACCE) was not different between the groups. At 1-year follow-up, target vessel revascularization (TVR) in both ticagrelor groups were significantly lower than that in the clopidogrel group (p = 0.047); TVR was significantly decreased in 60 mg ticagrelor compared to standard dose clopidogrel (p = 0.046). At 1-year follow-up, overall MACCE in both ticagrelor groups were significantly lower than that in the clopidogrel group (p = 0.023). Kaplan-Meier analysis showed MACCE-free survival was significantly higher in both ticagrelor groups than in the clopidogrel group (p = 0.024). During hospitalization, minor bleeding was significant increased in the 90 mg ticagrelor group (p = 0.021). At 1-year follow-up, risk of major and minor bleeding were significantly increased in the 90 mg ticagrelor group. CONCLUSION: In East Asian patients with CTO undergoing PCI, 60 mg ticagrelor was as effective as 90 mg, at the same time significantly reduced risk of bleeding.

9.
Small ; : e1907626, 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32187853

RESUMO

The counterfeiting of goods is growing worldwide, affecting practically any marketable item ranging from consumer goods to human health. Anticounterfeiting is essential for authentication, currency, and security. Anticounterfeiting tags based on structural color materials have enjoyed worldwide and long-term commercial success due to their inexpensive production and exceptional ease of percept. However, conventional anticounterfeiting tags of holographic gratings can be readily copied or imitated. Much progress has been made recently to overcome this limitation by employing sufficient complexity and stimuli-responsive ability into the structural color materials. Moreover, traditional processing methods of structural color tags are mainly based on photolithography and nanoimprinting, while new processing methods such as the inkless printing and additive manufacturing have been developed, enabling massive scale up fabrication of novel structural color security engineering. This review presents recent breakthroughs in structural color materials, and their applications in optical encryption and anticounterfeiting are discussed in detail. Special attention is given to the unique structures for optical anticounterfeiting techniques and their optical aspects for encryption. Finally, emerging research directions and current challenges in optical encryption technologies using structural color materials is presented.

10.
Photodiagnosis Photodyn Ther ; : 101745, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32198021

RESUMO

Antifungal medicine is the recommended therapy of tinea capitis. However, oral antifungal medicine could cause liver dysfunction to some extent, especially in children. This report shared a case of tinea capitis child suffering from liver dysfunction after taking antifungal drugs and having recovered after photodynamic therapy (PDT), which indicated PDT as a safe and effective therapy of tinea capitis in children.

11.
Artigo em Inglês | MEDLINE | ID: mdl-32198676

RESUMO

Simulated microgravity can significantly affect various cell types and multiple systems of the human body, such as cardiovascular system, skeletal muscle system, and immune system, and is known to cause anemia and loss of electrolyte and fluids. Epidermal stem cells (EpSCs) were cultured in a rotary cell culture system (RCCS) bioreactor to simulate microgravity. The metabolites of EpSCs were identified by liquid chromatography-mass spectrometry (LC-MS). Compared with normal gravity (NG) group, a total of 57 different metabolites of EpSCs were identified (P < 0.05, VIP > 1), including lipids and lipid-like molecules (51 molecules), amino acids (5 molecules), nucleosides, nucleotides, and analogues (1 molecule). According to the partial least squares discriminant analysis (PLS-DA) score plot, a VIP > 1 and P < 0.05 were obtained for the 57 different metabolites, of which 23 molecules were significantly downregulated and 34 were significantly upregulated in simulated microgravity (SMG) group. These results showed that SMG has a significant impact on different pathways, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that multiple pathways were involved, mainly the amino acid metabolism pathway, lipid metabolism pathway, membrane transport pathway, and cell growth and death pathways. Thus, the metabolic profile of EpSCs was changed under SMG. Exploring the metabolic profile of EpSCs would be helpful to further understand the growth characteristics of EpSCs under SMG, which will provide a new approach to explore the metabolomics mechanism of stress injury and repair trauma under SMG.

12.
J Integr Plant Biol ; 2020 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-32198820

RESUMO

Hormones are important signaling molecules regulating developmental processes and responses to environmental stimuli in higher plants. Rice endosperm, the portion of the seed surrounding the embryo, is the main determinant of rice grain shape and yield; however, the dynamics and exact functions of phytohormones in developing endosperm remain elusive. Through a systemic study including transcriptome analysis, hormone measurement, and transgene-based endosperm-specific expression of phytohormone biosynthetic enzymes, we demonstrated that dynamic phytohormone levels play crucial roles in the developing rice endosperm, particularly in regard to grain shape and quality. We detected diverse, differential, and dramatically changing expression patterns of genes related to hormone biosynthesis and signaling during endosperm development, especially at early developmental stages. Liquid chromatography measurements confirmed the dynamic accumulation of hormones in developing endosperm. Further transgenic analysis performed on plants expressing hormone biosynthesis genes driven by an endosperm-specific promoter revealed differential effects of the hormones, especially auxin and brassinosteroids, in regulating grain shape and quality. Our studies help elucidate the distinct roles of hormones in developing endosperm and provide novel and useful tools for influencing crop seed shape and yield. This article is protected by copyright. All rights reserved.

13.
Oxid Med Cell Longev ; 2020: 3076131, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32190170

RESUMO

Objectives: Our previous study showed that aldose reductase (AR) played key roles in fatty liver ischemia-reperfusion (IR) injury by regulating inflammatory response and energy metabolism. Here, we aim to investigate the role and mechanism of AR in the regeneration of normal and fatty livers after liver surgery. Methods: The association of AR expression with liver regeneration was studied in the rat small-for-size liver transplantation model and the mice major hepatectomy and hepatic IR injury model with or without fatty change. The direct role and mechanism of AR in liver regeneration was explored in the AR knockout mouse model. Results: Delayed regeneration was detected in fatty liver after liver surgery in both rat and mouse models. Furthermore, the expression of AR was increased in liver after liver surgery, especially in fatty liver. In a functional study, the knockout of AR promoted liver regeneration at day 2 after major hepatectomy and IR injury. Compared to wild-type groups, the expressions of cyclins were increased in normal and fatty livers of AR knockout mice. AR inhibition increased the expressions of PPAR-α and PPAR-γ in both normal liver and fatty liver groups after major hepatectomy and IR injury. In addition, the knockout of AR promoted the expressions of SDHB, AMPK, SIRT1, and PGC1-α and PPAR. Conclusions: The knockout of AR promoted the regeneration of normal and fatty livers through regulating energy metabolism. AR may be a new potential therapeutic target to accelerate liver regeneration after surgery.

14.
Talanta ; 213: 120850, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32200917

RESUMO

Specific detection of Plasmodium vivax lactate dehydrogenase (PvLDH), an important biomarker of malaria, remains a significant challenge. Herein, adenosine monophosphate protected gold-silver bimetallic nanoclusters, Au-AgNCs@AMP were used as a specific and sensitive fluorescence probe to detect PvLDH. After optimizing, a linear response was shown over a wide concentration range (10-100 nM) and an extremely low limit of detection (LOD) at 0.10 nM (3.7 ng mL-1) was achieved finally. Albeit the method was able to detect PvLDH sensitively, it could not discriminate different types of LDHs. Consequently, Al3+ was employed as an "assistant agent", which induced an assay capacity to discriminate PvLDH from other LDHs. The bimetallic nanoclusters inhibited the activity of PvLDH, suggesting it bound near the active site of PvLDH with high affinity. Zeta potential and UV-vis absorption experiments showed that electrostatic interaction was the main driving force for the interaction between the nanoclusters and PvLDH. Through chemical modification it indicated free thiol groups in PvLDH played an implant role in the interaction. Overall, the fluorescence enhancement and blue-shift were attributed to assembly-induced emission enhancement (AIEE) and hydrophobic transfer. The present study provides a simple, robust, and easy-to-perform approach to detect PvLDH with high sensitivity and selectivity, with significant potential for malaria diagnosis in the developing world.

15.
Artigo em Inglês | MEDLINE | ID: mdl-32111069

RESUMO

A genome-wide association study (GWAS) in the Han Chinese population had found that single nucleotide polymorphism (SNP) on the CMTM7 gene rs347134 was significantly associated with Body Mass Index (BMI). In the present study, the association of the rs347134 SNP with obesity and its interaction with dietary patterns (DPs) were explored in Han Chinese children. This cross-sectional study group included 1292 children, in whom obesity-related indicators were evaluated, the rs347134 SNP was genotyped by improved Multiple Ligase Detection Reaction (iMLDR), and the DPs were identified by principal component factor analysis. The GG genotype exhibited higher odds of general overweight/obesity (P = 0.038) and central obesity (P = 0.039) than AA+GA genotypes in boys. Four DPs of boys were identified: healthy balanced (HBDP), nuts and sweets-based (NSDP), animal food-based (AFDP), and wheaten and dairy-based (WDDP). Boys with the GG genotype were significantly more inclined to AFDP (P = 0.028) and had a shorter sleep duration (P = 0.031). Significant interactions were observed; boys with the GG genotype displayed a higher LDL in AFDP (P = 0.031) and higher FBG in NSDP (P = 0.038), respectively. Our findings indicate for the first time that the GG genotype of CMTM7 rs347134 is potentially a novel obesity risk factor for Han Chinese male children and is associated with dietary patterns more or less.

16.
Poult Sci ; 99(3): 1297-1305, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32111306

RESUMO

The symbiosis of host and intestinal microbiota constitutes a microecosystem and plays an important role in maintaining intestinal homeostasis and regulating the host's immune system. Eimeria tenella, an obligate intracellular apicomplexan parasite, can cause coccidiosis, a serious intestinal disease. In this study, the effects of E. tenella infection on development parameters (villus height, crypt depth, mucosa thickness, muscularis thickness, and serosa thickness) and microbiota in chicken cecum were investigated. Fourteen-day-old male Hy-Line Variety Brown layer chickens were inoculated with sporulated oocysts of E. tenella. Cecal tissues were collected 7 d after inoculation. Relative density of goblet cells and glycoproteins were determined by Alcian blue periodic acid-Schiff staining and periodic acid-Schiff staining, respectively. Intestinal development parameters were also evaluated. Cecal contents were extracted, and the composition of cecal microflora was examined by Illumine sequencing in the V3-V4 region of the 16S rRNA gene. Results indicated that E. tenella infection destroyed the structure of cecal tissue and reduced the relative density of goblet cells and glycoproteins. Sequencing analysis indicated that E. tenella infection altered the diversity and composition of cecal microbiota. The populations of Proteobacteria, Enterococcus, Incertae, and Escherichia-Shigella decreased, and those of Bacteroidales and Rikenella significantly increased in the infected group compared with those in the control group. Hence, the pathological damage caused by E. tenella infection is associated with cecal microbiota dysbiosis, and this finding may be used to develop an alternative measure for alleviating the effect of coccidiosis on the poultry industry.

17.
Org Lett ; 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32157891

RESUMO

A visible-light-driven, copper-catalyzed C(sp3)-O coupling of benzylic radicals with phenols is described for the first time. This operationally simple and robust process features mild conditions, broad scope, and high functional-group tolerance, enabling the construction of a diverse range of cyanoalkylated aryl ethers (33 examples). Key to this protocol is the dual role of copper salt that acts not only as a photosensitizer but also as a cross-coupling catalyst.

18.
Surg Endosc ; 2020 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-32144555

RESUMO

BACKGROUND: Despite the fact that thyroid surgery has evolved towards minimal incisions and endoscopic approaches, the role of total endoscopic thyroidectomy (TET) in thyroid cancer has been highly disputed. We performed a systematic review and meta-analyses of peer reviewed studies in order to evaluate the safety and effectiveness of TET compared with conventional open thyroidectomy (COT) in papillary thyroid cancer (PTC). METHOD: Medical literature databases such as PubMed, Embase, the Cochrane Library, and Web of science were systematically searched for articles that compared TET and COT in PTC treatment from database inception until March 2019. The quality of the studies included in the review was evaluated using the Downs and Black scale using Review Manager software Stata V.13.0 for the meta-analysis. RESULTS: The systematic review and meta-analysis were based on 5664 cases selected from twenty publications. Criteria used to determine surgical completeness included postoperative thyroglobulin (TG) levels, recurrence of the tumor after long-term follow-up. Adverse event and complication rate scores included transient recurrent laryngeal nerve (RLN) palsy, permanent RLN palsy, transient hypocalcaemia, permanent hypocalcaemia, operative time, number of removed lymph nodes, length of hospital stay and patient cosmetic satisfaction. TET was found to be generally equivalent to COT in terms of surgical completeness and adverse event rate, although TET resulted in lower levels of transient hypocalcemia (OR 1.66; p < 0.05), a smaller number of the retrieved lymph nodes (WMD 0.46; p < 0.05), and better cosmetic satisfaction (WMD 1.73; p < 0.05). COT was associated with a shorter operation time (WMD - 50.28; p < 0.05) and lower rates of transient RLN palsy (OR 0.41; p < 0.05). CONCLUSIONS: The results show that in terms of safety and efficacy, TET was similar to COT for the treatment of thyroid cancer. Indeed, the tumor recurrence rates and the level of surgical completeness in TET are similar to those obtained for COT. TET was associated with significantly lower levels of transient hypocalcemia and better cosmetic satisfaction, and thus is the better option for patients with cosmetic concerns. Overall, randomized clinical trials and studies with larger patient cohorts and long-term follow-up data are required to further demonstrate the value of the TET.

19.
Nat Chem Biol ; 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-32152538

RESUMO

The α2 adrenergic receptors (α2ARs) are G protein-coupled receptors (GPCRs) that respond to adrenaline and noradrenaline and couple to the Gi/o family of G proteins. α2ARs play important roles in regulating the sympathetic nervous system. Dexmedetomidine is a highly selective α2AR agonist used in post-operative patients as an anxiety-reducing, sedative medicine that decreases the requirement for opioids. As is typical for selective αAR agonists, dexmedetomidine consists of an imidazole ring and a substituted benzene moiety lacking polar groups, which is in contrast to ßAR-selective agonists, which share an ethanolamine group and an aromatic system with polar, hydrogen-bonding substituents. To better understand the structural basis for the selectivity and efficacy of adrenergic agonists, we determined the structure of the α2BAR in complex with dexmedetomidine and Go at a resolution of 2.9 Å by single-particle cryo-EM. The structure reveals the mechanism of α2AR-selective activation and provides insights into Gi/o coupling specificity.

20.
J Breath Res ; 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-32053807

RESUMO

Glycosylated hemoglobin (HbA1c) is an important criterion for the diagnosis of diabetes and indicator of blood glucose level. But the red blood cell (RBC) lifespan heterogeneity is sufficient to influence on HbA1c interpretation. In this study, we recruited 115 patients with diabetes mellitus and 81 nondiabetic controls. The HbA1c and the RBC lifespan was detected by high performance liquid chromatography and the advanced CO breath detection method, respectively. Potential correlations of gender and age with HbA1c were analyzed and a receiver operator characteristic (ROC) curve was generated to get the HbA1c cut-off for every RBC lifespan group. It was confirmed that HbA1c has no correlation with gender and age. And the correlation formula between HbA1c diagnostic criteria and RBC lifespan was derived to correct the HbA1c diagnostic criteria using the least-square method. The RBC-lifespan-corrected HbA1c diagnostic criteria provided 100% sensitivity and specificity for the diagnosis of diabetes in the experimental set and was not refuted in the validated set. The diagnostic value of HbA1c is positively correlated with the RBC lifespan, and 4 patients with hyperglycemia, whose HbA1c values are lower than the general diagnosis criterion 6.5%, were still considered to be diabetic according to this formula, that is, the application of this formula may help us to eliminate 2.2% misdiagnosis rate of the current diagnostic criteria. To provide more accurate detection results, the effect of RBC lifespan is necessary to be taken into account when HbA1c is used as a clinical indicator.

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