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1.
Clin Infect Dis ; 69(5): 861-864, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-30715242

RESUMO

Using metagenomics analysis, we are the first to identify the presence of a small, circular, single-stranded Gemykibivirus (GkV) genome from the respiratory tract of an elderly woman with severe acute respiratory distress syndrome. Our results suggest that further studies on whether GkVs infect humans and cause respiratory disease are needed.

2.
J Med Virol ; 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30485459

RESUMO

Understanding of kinetics of antibody responses is crucial for developing rapid serological tests and studying the mechanisms of Zika virus (ZIKV) infection. Most of the serological diagnostic assays previously published are based on either IgM or IgG titer, little is known on the level of IgA antibody in saliva and urine. In this study, we investigated the kinetics of IgM/ IgG/ IgA antibody responses in serum, saliva and urine obtained from two ZIKV infected individuals from as early as the 2nd day of onset of symptoms to as long as 2 years post infection. Other than detecting robust early IgM response, long lasting IgG response, we discovered strong early IgA response specific for ZIKV in saliva in both patients. This unique observation provides a novel strategy and scientific basis for the development of non-invasive rapid tests for ZIKV infection. This article is protected by copyright. All rights reserved.

3.
PLoS One ; 13(7): e0200478, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30044839

RESUMO

Antibody dependent enhancement (ADE) has most often been associated with dengue virus (DENV). Studies using leukemia cell lines suggest that DENV specific antibodies can enhance Zika virus (ZIKV) infectivity, and vice versa. To examine the mechanisms of ADE of ZIKV infection in primary human cells, we assessed 40 serum samples obtained from convalescent DENV-1 or DENV-3 infected subjects. All sera tested exhibited high binding potency, while modest or none neutralization activities against ZIKV. Primary CD14+ monocytes, rather than B and T cells in peripheral blood mononuclear cells (PBMCs), were found to be the mediators of the enhancement of ZIKV infectivity by DENV immune sera. Monocyte-derived immature dendritic cells (DCs), but not mature DCs were highly permissive to ZIKV infection, whereas neither immature nor mature DCs could mediate enhanced ZIKV infection in the presence of DENV immune sera. In addition, antibody blocking of either FcγRI (CD64), or FcγRII (CD32), or FcγRIII (CD16) resulted in diminished ADE of ZIKV infection. Our findings provide an improved understanding of the pathogenesis of ZIKV infection, and inform rational vaccine design.

4.
BMC Infect Dis ; 18(1): 275, 2018 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-29898684

RESUMO

BACKGROUND: Zika virus (ZIKV) had spread rapidly in the past few years in southern hemisphere where dengue virus (DENV) had caused epidemic problems for over half a century. The high degree of cross-reactivity of Envelope (E) protein specific antibody responses between ZIKV and DENV made it challenging to perform differential diagnosis between the two infections using standard ELISA method for E protein. METHODS: Using an IgG capture ELISA, we investigated the kinetics of nonstructural protein 1 (NS1) antibody response during natural ZIKV infection and the cross-reactivity to NS1 proteins using convalescent sera obtained from patients infected by either DENV or ZIKV. RESULTS: The analyses of the sequential serum samples from ZIKV infected individuals showed NS1 specific Abs appeared 2 weeks later than E specific Abs. Notably, human sera from ZIKV infected individuals did not contain cross-reactivity to NS1 proteins of any of the four DENV serotypes. Furthermore, four out of five NS1-specific monoclonal antibodies (mAbs) isolated from ZIKV infected individuals did not bind to DENV NS1 proteins. Only limited amount of cross-reactivity to ZIKV NS1 was displayed in 108 DENV1 immune sera at 1:100 dilution. CONCLUSIONS: The high degree of NS1-specific Abs in both ZIKV and DENV infection revealed here suggest that NS1-based diagnostics would significantly improve the differential diagnosis between DENV and ZIKV infections.

5.
JCI Insight ; 2(12)2017 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-28614803

RESUMO

Zika virus (ZIKV) is an emerging mosquito-transmitted flavivirus that shares a considerable degree of homology with dengue virus (DENV). Here, we examined longitudinal antibody response against ZIKV during natural infection in 2 convalescent individuals. By decomposing the antibody recognition into DI/DII and DIII of the E glycoprotein, we showed their development in humans followed a spatiotemporal hierarchy. Plasma binding to DI/DII appeared to peak and wane during early infection with extensive cross-reactivity with DI/DII of DENV. Binding to DIII, however, peaked early but persisted months into the infection without detectable cross-reactivity with DIII of DENV. A clear trend of increase in DIII-specific neutralizing activity was observed over the course of infection. mAbs isolated during early infection are largely DI/DII specific, weakly neutralizing, and highly cross-reactive with DENV, while those from later infection are more diverse in recognition, potently neutralizing, and ZIKV specific. The most potent neutralizing mAb targeting the DIII provided 100% protection in mice from lethal ZIKV infection and could therefore serve as a promising candidate for antibody-based therapy and prevention. The dynamic features unveiled here will assist us to better understand the pathogenesis of ZIKV infection and inform rational design of vaccines.

6.
Medicine (Baltimore) ; 95(27): e4051, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27399087

RESUMO

Myocarditis is a common complication of severe dengue infection. However, data about prevalence and characterization of myocarditis in dengue are still lacking. In 2014, the worst outbreak of dengue in the last two decades in China occurred. In this study, we described the clinical and laboratory diagnostic features of dengue with myocarditis. Totally, 1782 diagnosed dengue patients were admitted from August to October, 2014, all of whom were subjected to electrocardiogram, ultrasound cardiogram, and cardiac enzyme test. About 201 cases of dengue patients were diagnosed with myocarditis and the prevalence of myocarditis in hospitalized dengue was 11.28%. The prevalence of myocarditis in nonsevere dengue with warning signs and severe dengue [NSD(WS+)/SD] and nonsevere dengue without warning signs [NSD(WS-)] was 46.66% and 9.72%, respectively. The NSD(WS+)/SD patients with myocarditis presented with higher incidence of cardiac symptoms, supraventricular tachycardia (14.29% vs. 0%, P < 0.001), atrial fibrillation (25.71% vs. 10.24%, P = 0.019) and heart failure compared with NSD (WS-) patients with myocarditis. About 150 cases of dengue patients without myocarditis in the same period of time in department of Cardiology were recruited as control group. The proportion of NSD(WS+)/SD in dengue patients with and without myocarditis was 17.41% and 2.53%, respectively. Dengue patients with myocarditis experienced longer hospital stay than those without myocarditis (7.17 ±â€Š4.64 vs. 5.98 ±â€Š2.69, P = 0.008). There was no difference between patients with and without myocarditis in the proportion of symptoms, auxiliary methods abnormality, arrhythmia, and heart failure on the discharge day. Our study demonstrates the prevalence of myocarditis in worst outbreak of dengue in China was 11.28% and the incidence of myocarditis increased with the severity of dengue. The NSD(WS+)/SD patients with myocarditis presented with higher incidence of cardiac complication compared with NSD (WS-) patients with myocarditis. The prognosis of dengue patients with and without myocarditis had no significant difference even if myocarditis patients experienced longer hospital stay.


Assuntos
Dengue/complicações , Surtos de Doenças , Miocardite/virologia , Doenças Cardiovasculares/diagnóstico , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/virologia , China/epidemiologia , Dengue/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Incidência , Tempo de Internação/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Miocardite/diagnóstico , Miocardite/epidemiologia , Prevalência , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa
7.
PLoS One ; 11(6): e0156548, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27257804

RESUMO

Dengue used to be recognized as an imported and sporadic disease in China. Since June 2014, an unexpected large dengue outbreak has attacked Guangzhou, China, resulting in more than 40,000 cases. Among the 1,942 laboratory-confirmed hospitalized dengue cases, 121 were diagnosed as severe dengue according to the 2009 WHO guideline, and 2 patients finally died. Laboratory diagnosis and virus isolation demonstrated that the majority (96%) cases were caused by dengue virus serotype 1 (DENV-1), and the others by serotype 2 (DENV-2). 14 DENV strains were isolated from the sera of acute-phase dengue patients during this outbreak, and the complete envelope (E) gene of 12 DENV-1 strains and two DENV-2 strains were determined using RT-PCR assay. Phylogenetic analysis based on the E gene revealed the DENV-1 strains isolated during the outbreak belonged to genotype I and V, respectively. These isolates formed three clades. DENV-2 isolates were assigned to the same clade belonging to genotype cosmopolitan. These strains isolated in 2014 were closely related to the isolates obtained from the same province, Guangdong, in 2013. No amino acid mutations known to increase virulence were identified throughout the E protein of isolates in 2014. These results indicate that dengue is turning into endemic in Guangdong, China, and extensive seroepidemiological investigation and mosquito control measures are critically needed in the future.


Assuntos
Dengue/epidemiologia , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Vírus da Dengue/classificação , Vírus da Dengue/genética , Vírus da Dengue/patogenicidade , Surtos de Doenças/estatística & dados numéricos , Feminino , Genoma Viral/genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Filogenia , Análise de Sequência de DNA , Sorogrupo
8.
BMC Infect Dis ; 16: 291, 2016 06 14.
Artigo em Inglês | MEDLINE | ID: mdl-27301555

RESUMO

BACKGROUND: The pathogenesis of severe dengue has not been fully elucidated. The inflammatory response plays a critical role in the outcome of dengue disease. METHODS: In this study, we investigated the levels of 17 important inflammation mediators in plasma collected from mild or severe adult dengue patients at different time points to understand the contribution of inflammation to disease severity and to seek experimental evidence to optimize the existing clinical treatment strategies. Patients were simply classified as mild and severe dengue according to the 2009 WHO classification. Plasma was collected on day 3-5, 6-7, 8-10 and 14-17 of illness. Levels of 17 inflammation mediators including TNF-α, IL-1α, IFN-γ, IL-6, IFN-α, MIF, IL-10, IL-1RA, IL-8, IP-10, MCP-1, RANTES, GRO, eotaxin-1, sICAM-1 and sVCAM-1 were determined by a multiplex Luminex® system. Different trends of inflammation mediators throughout the disease were compared between mild and severe patients. RESULTS: Inflammation mediators including IL-1α, IFN-γ, IL-10, IL-8, IP-10, MCP-1 and sVCAM-1 displayed significant differences on day 8-10 of illness between mild and severe dengue patients. Their concentrations were higher in severe patients than mild ones at the same time points. Moreover, those cytokines decreased gradually in mild patients but not in severe patients. CONCLUSION: Our results revealed the coexistence of excessive inflammatory response and slow resolution of inflammation in severe adult dengue patients. Hence suppression and/or pro-resolution of inflammation could be a potential therapeutic approach for treatment of severe dengue.


Assuntos
Citocinas/imunologia , Dengue Grave/imunologia , Adolescente , Adulto , Estudos de Casos e Controles , Quimiocina CCL11/imunologia , Quimiocina CCL2/imunologia , Quimiocina CCL5/imunologia , Quimiocina CXCL1/imunologia , Feminino , Humanos , Inflamação , Mediadores da Inflamação , Molécula 1 de Adesão Intercelular/imunologia , Interferon-alfa/imunologia , Interferon gama/imunologia , Proteína Antagonista do Receptor de Interleucina 1/imunologia , Interleucina-10/imunologia , Interleucina-1alfa/imunologia , Interleucina-6/imunologia , Interleucina-8/imunologia , Oxirredutases Intramoleculares/imunologia , Fatores Inibidores da Migração de Macrófagos/imunologia , Masculino , Pessoa de Meia-Idade , Índice de Gravidade de Doença , Fatores de Tempo , Fator de Necrose Tumoral alfa/imunologia , Molécula 1 de Adesão de Célula Vascular/imunologia , Adulto Jovem
9.
Sci Rep ; 6: 27298, 2016 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-27250703

RESUMO

The kinetics of dengue virus (DENV)-specific IgA antibody in urine and the potential correlation with disease severity remain elusive. In this study, 262 serial urine samples from 78 laboratory-confirmed patients were assayed by a commercial immunoglobulin A (IgA) kit against DENV. All cases were classified into dengue fever (DF) and severe dengue (SD) according to the 2009 WHO/TDR guideline. The total positive rate of IgA in urine was 59%. DENV-specific IgA was detected in urine from day 2 to day 13 after the onset of illness in DF patients; While for SD patients, anti-DENV IgA could be detected till day 14. The positive rate of IgA in patients with secondary infection was higher than that in patients with primary infection. Importantly, during 4-7 days after the onset of illness, the IgA positive rate of SD patients was significantly higher than that of DF patients. Especially, the intensity of IgA signal in SD patients was obviously stronger than that in DF patient at the recovery stage. Overall, our results suggested that the existence of DENV-specific IgA antibodies in urine might be a warning sign for the severity of disease and its measurement might provide valuable guidance for proper patient management.


Assuntos
Anticorpos Antivirais/urina , Vírus da Dengue/imunologia , Dengue/imunologia , Dengue/patologia , Imunoglobulina A/urina , Adulto , Idoso , Idoso de 80 Anos ou mais , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
10.
Emerg Microbes Infect ; 4(4): e24, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26421267

RESUMO

The damage of vascular endothelial cells has been speculated to be involved in the pathogenesis of dengue virus (DENV) infection. However, little is known about the role of soluble vascular cell adhesion molecule-1 (sVCAM-1) in predicting the severity of dengue infection in adults. In this study, 51 adults with DENV-1 infection (21 with severe dengue and 30 with dengue fever (DF) were included, and their serum levels of sVCAM-1 and other parameters were determined. The results indicated that the levels of sVCAM-1 were elevated on days 1-3 to 16.75 (11.55-34.74) ng/mL in the severe dengue patients. These levels increased rapidly to peak values of 43.53 (37.15-47.02) ng/mL on days 10-12 and then declined; however, the values were maintained at a high level (38.07 (26.06-39.63) ng/mL). Other parameters, including reduced platelet (PLT) counts, neutrophil (NEU) counts and increased levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK), were also observed in the severe dengue group but not in the DF group. The levels of cytokines, such as interleukin (IL)-6, IL-10, interferon γ (IFNγ), and tumor necrosis factor α (TNF-α), were transiently increased in the severe dengue patients. Among the aforementioned parameters, only sVCAM-1 levels were significantly elevated earlier and more persistently in the severe dengue patients than in the DF patients. sVCAM-1 positively correlated with the levels of ALT, AST, LDH, TNF-α, and IL-6 and negatively correlated with the levels of PLT, NEU, and viremia. Notably, the high levels of sVCAM-1 were closely associated with the severe dengue patients. In conclusion, sVCAM-1 may be a superior indicator for monitoring the severity of dengue.


Assuntos
Vírus da Dengue/classificação , Dengue/sangue , Molécula 1 de Adesão de Célula Vascular/sangue , Adulto , Distribuição por Idade , Biomarcadores/sangue , Citocinas/sangue , Vírus da Dengue/genética , Vírus da Dengue/patogenicidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , RNA Viral/sangue , Fatores de Risco , Índice de Gravidade de Doença , Razão de Masculinidade , Viremia/sangue , Adulto Jovem
11.
Sci China Life Sci ; 57(12): 1149-55, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25502398

RESUMO

Dengue has been well recognized as a global public health threat, but only sporadic epidemics and imported cases were reported in recent decades in China. Since July 2014, an unexpected large dengue outbreak has occurred in Guangdong province, China, resulting in more than 40000 patients including six deaths. To clarify and characterize the causative agent of this outbreak, the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing (HTS). Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2 (DENV-2). A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells. The complete genome of GZ05/2014 was determined and deposited in GenBank under the access number KP012546. Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade. No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014. The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong, China. These results help clarify the origin of this epidemic and predict the future status of dengue in China.


Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/epidemiologia , Surtos de Doenças , Idoso , Idoso de 80 Anos ou mais , Animais , China/epidemiologia , Culicidae/virologia , Dengue/transmissão , Dengue/virologia , Feminino , Ensaios de Triagem em Larga Escala , Humanos , Insetos Vetores , Reação em Cadeia da Polimerase em Tempo Real
12.
Int J Infect Dis ; 27: 4-6, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25107464

RESUMO

In recent decades, the impact of dengue has increased both geographically and in intensity, and this disease is now a threat to approximately half of the world's population. An unexpected large outbreak of dengue fever was reported in Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, China, in 2013. This was the first autochthonous outbreak with a significant proportion of severe dengue cases in mainland China in a decade. According to the 2009 World Health Organization guidelines, half of the 136 laboratory confirmed cases during the epidemic were severe dengue. The clinical presentation included severe haemorrhage (such as massive vaginal and gastrointestinal bleeding), severe plasma leakage (such as pleural effusion, ascites, or hypoproteinaemia), and organ involvement (such as myocarditis and lung impairment); 21 cases eventually deteriorated to shock. During this outbreak, all severe cases occurred in adults, among whom about 43% had co-morbid conditions. Nucleic acid detection and virus isolation confirmed dengue virus serotype 3 (DENV-3) to be the pathogenic agent of this outbreak. Phylogenetic analyses of envelope gene sequences showed that these DENV-3 isolates belonged to genotype II. This finding is of great importance to understand the circulation of DENV and predict the risk of severe disease in mainland China. Here, we provide a brief report of the epidemiology, clinical manifestations, and aetiology of this dengue fever outbreak, and characterize DENV strains isolated from clinical specimens.


Assuntos
Dengue/epidemiologia , Surtos de Doenças , Adolescente , Adulto , China/epidemiologia , Dengue/patologia , Dengue/virologia , Vírus da Dengue/classificação , Vírus da Dengue/isolamento & purificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
14.
J Virol ; 86(24): 13808-9, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23166230

RESUMO

Here we report the complete genome sequence of a dengue virus serotype 2 (DENV-2) strain, GZ40, isolated in Guangdong, China, in 2010. A phylogenetic analysis classified GZ40 into the Cosmopolitan genotype, while previous Chinese DENV-2 isolates belong to the Asian I genotype. The reemergence of the Cosmopolitan genotype of DENV-2 in China deserves further investigation.


Assuntos
Vírus da Dengue/genética , Genoma Viral , China , Vírus da Dengue/classificação , Dados de Sequência Molecular
15.
Virol J ; 9: 125, 2012 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-22721418

RESUMO

Dengue is emerging as the most important mosquito borne viral disease in the world. In mainland China, sporadic and large outbreaks of dengue illness caused by the four serotypes of dengue virus (DENV-1 to DENV-4) have been well documented. Guangdong province is the major affected area in China, and DENV-1 has dominantly circulated in Guangdong for a long time. In this study, a family cluster of DENV-3 infection in Guangzhou was described. Three cases were diagnosed as dengue fever based on clinical manifestation, serological and RT-PCR assays. Two DENV-3 strains were isolated in C6/36 cells and the complete genome sequences were determined. Phylogenetic analysis revealed that the new DENV-3 isolates from the family cluster were grouped within genotype III. Considering the fact that several DENV-3 strains within genotype V were also identified in Guangzhou in 2009, at least two genotypes of DENV-3 co-circulated in Guangzhou. Careful investigation and virological analysis should be warranted in the future.


Assuntos
Vírus da Dengue/classificação , Vírus da Dengue/genética , Dengue/epidemiologia , Dengue/virologia , Adulto , Anticorpos Antivirais/sangue , China/epidemiologia , Análise por Conglomerados , Dengue/patologia , Vírus da Dengue/isolamento & purificação , Saúde da Família , Feminino , Genoma Viral , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Dados de Sequência Molecular , Filogenia , RNA Viral/genética , RNA Viral/isolamento & purificação , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA
16.
J Virol ; 86(12): 7021-2, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22628403

RESUMO

Here we report the first complete genome sequence of a dengue virus serotype 4 genotype II strain, GZ30, isolated in Guangzhou, Guangdong Province, China, in 2010. The sequence information provided herein will help us to understand the molecular epidemiology of dengue virus and predict the risk of severe diseases in mainland China.


Assuntos
Vírus da Dengue/genética , Dengue/virologia , Genoma Viral , Sequência de Bases , China , Vírus da Dengue/classificação , Vírus da Dengue/isolamento & purificação , Feminino , Humanos , Pessoa de Meia-Idade , Dados de Sequência Molecular , Filogenia
17.
Artigo em Chinês | MEDLINE | ID: mdl-17653311

RESUMO

OBJECTIVE: To study the clinical characteristics of the patients with dengue fever (DF) seen from 2002 to 2006 in Guangzhou in order to prevent and treat dengue fever better. METHODS: Clinical data from 1342 inpatients with DF seen from 2002 to 2006 were retrospectively analyzed. The dengue virus was isolated by C6/36 cell culture and genotyped by reverse transcriptase-polymerase chain reaction and gene sequence analysis. RESULTS: The average age of the patients was 34.4 years, without sex difference in distribution. Most of the patients had obvious toxemic symptoms including fever (100 percent), headache (85.9 percent), myalgia (64.5 percent), bone soreness (46.6 percent) and skin rash (65.9 percent). Leukopenia, thrombocytopenia, elevated alanine aminotransferase, elevated aspartate aminotransferase and hypokalemia were found in 66.0 percent, 61.3 percent, 69.0 percent , 85.7 percent and 28.4 percent of patients, respectively. DF-IgM could be detected in 90 percent of patients. The virus was identified as dengue virus type-I. CONCLUSIONS: The epidemic of DF was caused by dengue virus- I from 2002 to 2006 in Guangzhou. Most of the patients had classic DF clinical manifestation with high percentage of hepatic injury. Few patients progressed to dengue hemorrhagic fever.


Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/epidemiologia , Dengue/fisiopatologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticorpos Antivirais/sangue , Criança , Pré-Escolar , China/epidemiologia , Dengue/diagnóstico , Dengue/imunologia , Vírus da Dengue/genética , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Adulto Jovem
18.
Zhonghua Liu Xing Bing Xue Za Zhi ; 26(6): 421-3, 2005 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16185453

RESUMO

OBJECTIVE: To analyze the clinical and epidemiological characteristics of Dengue fever (DF) during the Dengue-1 epidemic in Guangzhou. METHODS: Clinical and epidemiological data of 1032 patients with DF from May 2002 to November 2003 were retrospectively analyzed. Dengue virus were isolated by cell culture and typed by reverse transcriptase polymerase chain reaction (RT-PCR). RESULTS: Age of the patients ranged from 55 days to 91 years old (average 34.7 +/- 13.2 years) with sex ratio 1.03:1. Incubation period ranged from 2 to 12 days with mean periods of 5.3 +/- 2.4 days. Most (45.0%) cases appeared in September and the epidemic last from July to November. Dengue outbreak had involved 675 cases in 26 common places. The common manifestations were seen as fever (100%), headache (90.9%), myalgia (68.4%), bone soreness (48.8%), fatigue (79.3%), skin rash (60.1%), positive tourniquet test (45.3%), leukopenia (63.3%) and thrombocytopenia (60.8%), respectively. Dengue virus was isolated from serum of 19 out of 54 patients' and identified as Dengue virus type 1. DNA sequence analyzes on rates of nucleotide homology were 97%, 97% and 98% compared with those of Dengue virus type 1 strain of DF outbreak in Cambodia, in 1997 and 1999 in China. CONCLUSION: The epidemic of DF in Guangzhou in 2002/2003 was caused by Dengue virus type-1 with most patients showing classic type of the disease. Date suggested that change can happen from non-endemic to hypoendemic regions in Guangdong province.


Assuntos
Vírus da Dengue/isolamento & purificação , Dengue/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Sequência de Bases , Criança , Pré-Escolar , China/epidemiologia , Vírus da Dengue/classificação , Vírus da Dengue/genética , Feminino , Humanos , Incidência , Lactente , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular
19.
Zhonghua Nei Ke Za Zhi ; 42(7): 458-60, 2003 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-12921602

RESUMO

OBJECTIVE: To explore the myocardiac injury in patients with severe acute respiratory syndrome (SARS) and its clinical significance. METHODS: 37 SARS patients fulfilled the Guangdong provincial diagnostic criteria for infectious atypical pneumonia and 35 health controls were investigated. The serum levels of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST), troponin I (TnI), creatine kinase-MB (CK-MB) and myoglobin (MYO) were measured. RESULTS: CK, LDH and AST levels in patients were higher than those of control group (P < 0.01); furthermore, among patients the levels were higher in fatal cases than in survivors. The positive rates of TnI, CK-MB and MYO in patients were higher than those in controls (P < 0.05). CONCLUSION: The patients with SARS are subject to complicating myocardiac injury. Therefore, careful monitoring of the myocardiac enzyme profiles is of great importance in reducing the complications and mortality in patients with SARS.


Assuntos
Cardiomiopatias/diagnóstico , Síndrome Respiratória Aguda Grave/complicações , Adolescente , Adulto , Idoso , Aspartato Aminotransferases/sangue , Cardiomiopatias/etiologia , Creatina Quinase/sangue , Creatina Quinase Forma MB , Feminino , Humanos , Isoenzimas/sangue , L-Lactato Desidrogenase/sangue , Masculino , Pessoa de Meia-Idade , Mioglobina/sangue , Troponina I/sangue
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