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1.
Ecotoxicology ; 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33432458

RESUMO

Long-term frequent tillage would cause black soil degradation and serious soil erosion as soil microbial communities and soil structure are extremely sensitive to tillage process. However, there is no unified conclusion on the relationship between the distribution of soil water-stable aggregates (WSAs), and microbial community construction and diversity under long-term tillage in black soil during different seasons. In this study, we used wet-sieving method to evaluate the composition and stability of soil WSAs and employed Illumina MiSeq high-throughput sequencing technology to study the diversity, taxonomic composition and co-occurrence network properties of microbial community, comparing outcomes between uncultivated soil and long-term cultivated soil for 60 years in Keshan farm of Heilongjiang Province. The results showed that after long-term tillage, the proportion of larger than 1 mm WSAs reduced by 34.17-51.37%, and the stability of WSAs, soil pH, organic matter (OM), total nitrogen (TN) contents decreased significantly in all seasons (P < 0.05), while soil available phosphorus (AP) and available potassium (AK) contents increased remarkably (P < 0.05). The diversity of bacteria increased, while that of fungi decreased. Soil fungal communities were more susceptible to long-term tillage than bacterial and archaeal communities. Actinobacteria mainly exist in large WSAs (˃1 mm), and when their relative abundance is high, it is beneficial to improve the water-stability of black soil; while Proteobacteria and Gemmatimonadetes may exist in small WSAs (˂1 mm), whose high relative abundance will weaken the water-stability of black soil. The experimental results provide a scientific theoretical basis for sustainable utilization of black soil.

2.
Proc Natl Acad Sci U S A ; 117(41): 25712-25721, 2020 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-32989127

RESUMO

Vogt-Koyanagi-Harada (VKH) disease is a systemic autoimmune disorder affecting multiple organs, including eyes, skin, and central nervous system. It is known that monocytes significantly contribute to the development of autoimmune disease. However, the subset heterogeneity with unique functions and signatures in human circulating monocytes and the identity of disease-specific monocytic populations remain largely unknown. Here, we employed an advanced single-cell RNA sequencing technology to systematically analyze 11,259 human circulating monocytes and genetically defined their subpopulations. We constructed a precise atlas of human blood monocytes, identified six subpopulations-including S100A12, HLA, CD16, proinflammatory, megakaryocyte-like, and NK-like monocyte subsets-and uncovered two previously unidentified subsets: HLA and megakaryocyte-like monocyte subsets. Relative to healthy individuals, cellular composition, gene expression signatures, and activation states were markedly alternated in VKH patients utilizing cell type-specific programs, especially the CD16 and proinflammatory monocyte subpopulations. Notably, we discovered a disease-relevant subgroup, proinflammatory monocytes, which showed a discriminative gene expression signature indicative of inflammation, antiviral activity, and pathologic activation, and converted into a pathologic activation state implicating the active inflammation during VKH disease. Additionally, we found the cell type-specific transcriptional signature of proinflammatory monocytes, ISG15, whose production might reflect the treatment response. Taken together, in this study, we present discoveries on accurate classification, molecular markers, and signaling pathways for VKH disease-associated monocytes. Therapeutically targeting this proinflammatory monocyte subpopulation would provide an attractive approach for treating VKH, as well as other autoimmune diseases.

3.
Ocul Surf ; 18(4): 783-794, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32735949

RESUMO

PURPOSE: Dry eye disease (DED) is a common and multifactor-induced autoimmune ocular surface disease. Environmental factors, such as desiccating stress (DS) and hyperosmolarity, affect the corneal epithelium to induce ocular surface inflammation in DED. We aimed to explore the potential mechanisms by which innate immunity and pyroptosis are initiated in the mucosal epithelium in response to environmental stress. METHODS: Experimental dry eye was established in C57BL/6 J mice and genetic mice on the background of C57BL/6 J mice by subcutaneous injection of scopolamine and exposure to a desiccating environment. SDHCEC cell line was subjected to hyperosmolarity stress (450 mOsM). The phenol red thread tear test and corneal epithelial defects evaluation were used as assessments of severity of DED. RNA-sequencing, quantitative real-time PCR, western blotting and immunofluorescence staining were performed in this study. RESULTS: Loss-of-function studies indicated that genetic deletion of GSDMD alleviates DS-induced corneal epithelium defects, and GSDMD is needed for IL-33 processing. We further found that NLRP12 collaborates with NLRC4 inflammasome to initiate GSDMD-dependent pyroptosis, which requires TLR4-induced caspase-8 (CASP8) activation in the mucosal corneal epithelium in response to DS. CONCLUSIONS: These findings provide compelling evidence that GSDMD-dependent pyroptosis plays a pivotal role in DED. A novel mechanism involving NLRP12 and NLRC4 inflammasomes-induced GSDMD-dependent pyroptosis, accompanied by IL-33 processing is responsible for ocular surface epithelial defects in response to environmental stress. GSDMD is required for IL-33 processing and the subsequent amplification of inflammatory cascades. These findings reveal novel therapeutic targets for treating DED.

4.
Mol Neurodegener ; 15(1): 26, 2020 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-32295623

RESUMO

BACKGROUND: Acute glaucoma, characterized by a sudden elevation in intraocular pressure (IOP) and retinal ganglion cells (RGCs) death, is a major cause of irreversible blindness worldwide that lacks approved effective therapies, validated treatment targets and clear molecular mechanisms. We sought to explore the potential molecular mechanisms underlying the causal link between high IOP and glaucomatous RGCs death. METHODS: A murine retinal ischemia/ reperfusion (RIR) model and an in vitro oxygen and glucose deprivation/reoxygenation (OGDR) model were used to investigate the pathogenic mechanisms of acute glaucoma. RESULTS: Our findings reveal a novel mechanism of microglia-induced pyroptosis-mediated RGCs death associated with glaucomatous vision loss. Genetic deletion of gasdermin D (GSDMD), the effector of pyroptosis, markedly ameliorated the RGCs death and retinal tissue damage in acute glaucoma. Moreover, GSDMD cleavage of microglial cells was dependent on caspase-8 (CASP8)-hypoxia-inducible factor-1α (HIF-1α) signaling. Mechanistically, the newly identified nucleotide-binding leucine-rich repeat-containing receptor (NLR) family pyrin domain-containing 12 (NLRP12) collaborated with NLR family pyrin domain-containing 3 (NLRP3) and NLR family CARD domain-containing protein 4 (NLRC4) downstream of the CASP8-HIF-1α axis, to elicit pyroptotic processes and interleukin-1ß (IL-1ß) maturation through caspase-1 activation, facilitating pyroptosis and neuroinflammation in acute glaucoma. Interestingly, processing of IL-1ß in turn magnified the CASP8-HIF-1α-NLRP12/NLRP3/NLRC4-pyroptosis circuit to accelerate inflammatory cascades. CONCLUSIONS: These data not only indicate that the collaborative effects of NLRP12, NLRP3 and NLRC4 on pyroptosis are responsible for RGCs death, but also shed novel mechanistic insights into microglial pyroptosis, paving novel therapeutic avenues for the treatment of glaucoma-induced irreversible vision loss through simultaneously targeting of pyroptosis.

5.
Opt Express ; 27(4): 5065-5082, 2019 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-30876111

RESUMO

Enhanced photonic microwave generation by using a filtered optical feedback in an optically injected semiconductor laser operating at period-one (P1) dynamics is numerically demonstrated. In the simulation, the frequency tunability of the generated narrow-linewidth photonic microwave with the filtered optical feedback has been investigated. The results show that the frequency of the narrow-linewidth photonic microwave can be widely tuned by adjusting the injection parameters only or adjusting both the injection parameters and the center frequency of the filter. Moreover, the influence of the delay time, feedback strength, filter bandwidth and detuning on the linewidth, side-peak suppression and phase noise of the generated microwave have also been investigated in detail. The results show that with increasing feedback strength or delay time, evident reduction of the linewidth is observed. The side-peak suppression also increases with increasing feedback strength; however, side-peak suppression decreases with increasing feedback delay time. In addition, the linewidth reduction and side-peak suppression are relatively robust to the filter detuning, especially for higher feedback strengths and microwave frequencies. This is mainly attributed to the self-adaptive shifting of the red-shifted cavity resonance frequency to the center frequency of the filter in the FOF configuration.

6.
Opt Lett ; 43(17): 4184-4187, 2018 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-30160747

RESUMO

A narrow-linewidth single-frequency photonic-microwave-generation scheme using an optically injected semiconductor laser with a filtered optical feedback has been proposed. The filtered feedback comes from a single feedback loop, which includes a narrow bandpass filter. With the filtered feedback, the linewidth of the generated microwave can be significantly reduced from 22.4 MHz to 9.0 kHz, with the side peaks suppression of 28 dB. The proposed scheme shows superior performance compared to the conventional single-feedback configuration in terms of linewidth reduction and side peaks suppression. The proposed scheme also achieves better results compared to the complex dual-feedback setting. The mechanism for a better performance of filtered optical feedback is that the filtered feedback can effectively limit the external cavity modes and stabilize the period-one dynamics. In addition, the microwave linewidth decreases with the increase of the filter width until the optimized filter width is reached. Furthermore, the linewidth reduction and the side peaks suppression of a photonic microwave using filtered optical feedback is relatively insensitive to the frequency detuning between the filter center frequency and the free-running frequency of the semiconductor laser.

7.
Exp Eye Res ; 176: 59-68, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30008389

RESUMO

Alkali burn (AB) is one of the most serious ocular traumas in the world, characterized by extreme ocular surface disorders, critical secondary dry eye and irreversible vision loss. The exact mechanisms involved are unknown. Innate immunity, including the involvement of Toll-like receptors (TLRs) and NOD-like receptors (NLRs), is believed to participate in the pathogenesis of the epithelia, but the exact mechanisms by which TLRs transduce signals to NLRs and downstream molecules to initiate innate immunity remain poorly defined. In this present study, we used murine models of AB and AB concomitant desiccating stress (DS) to investigate the potential functions and mechanisms of TLR4 in regulating NLRP3 and NLRP6 during AB injury and secondary dry eye. We demonstrated that AB injury induced activation of the TLR4-MyD88 pathway, leading to imbalanced NLRP3 and NLRP6 via the activation of caspase-8 signaling. DS worsened ocular surface disorders post-AB injury by magnifying this phenomenon. Caspase-8 signaling promoted NLRP3 upregulation via the nuclear factor (NF)-κB pathway, while NLRP6 suppressed NF-κB activation. Our findings also revealed that TLR4-MyD88 knockout can alleviate AB-induced or DS-worsened ocular surface disorders, shedding light on the potential therapeutic strategies in the future for AB injury. Taken together, our findings demonstrate that AB promotes the TLR4-MyD88-caspase-8 axis to cause imbalanced NLRP3/NLRP6, and DS exacerbates ocular surface damage via magnifying this imbalance.


Assuntos
Queimaduras Químicas/metabolismo , Caspase 8/metabolismo , Traumatismos Oculares/induzido quimicamente , Fator 88 de Diferenciação Mieloide/fisiologia , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Receptores de Superfície Celular/metabolismo , Receptor 4 Toll-Like/fisiologia , Animais , Western Blotting , Modelos Animais de Doenças , Traumatismos Oculares/metabolismo , Técnica Indireta de Fluorescência para Anticorpo , Glibureto/farmacologia , Humanos , Hipoglicemiantes/farmacologia , Imuno-Histoquímica , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteína 3 que Contém Domínio de Pirina da Família NLR/genética , RNA Interferente Pequeno/genética , Reação em Cadeia da Polimerase em Tempo Real , Receptores de Superfície Celular/genética , Transdução de Sinais/fisiologia , Hidróxido de Sódio
8.
Opt Express ; 25(17): 19863-19871, 2017 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-29041673

RESUMO

Photonic microwave generation based on period-one dynamics of an optically injected VCSEL has been study experimentally. The results have shown that the frequency of the generated microwave signal can be broadly tunable through the adjustment of the injection power and the frequency detuning. Strong optical injection power and higher frequency detuning are favorable for obtaining a high frequency microwave signal. These results are similar to those found in systems based on distributed feedback lasers and quantum dot lasers. The variation of the microwave power at the fundamental frequency and the second-harmonic distortion have also been characterized.

9.
Opt Lett ; 42(13): 2507-2510, 2017 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-28957271

RESUMO

The effect of data acquisition on the complexity of chaos using permutation entropy has been studied experimentally and numerically. The complexity of chaos is quantified using the normalized permutation entropy at the feedback round-trip time. Two "abnormal" variations of the complexity of chaos with bias current have been observed experimentally. The different vertical scales of the oscilloscope in data acquisition are attributed to this abnormal change. The method to remove the effect of data acquisition has also been proposed.

11.
Opt Lett ; 41(11): 2664-7, 2016 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-27244440

RESUMO

Polarization switching in a long-wavelength vertical-cavity surface-emitting laser (VCSEL) under parallel optical injection is analyzed in a theoretical and experimental way. For the first time, to our knowledge, we report experimentally a state in which injection locking of the parallel polarization and excitation of the free-running orthogonal polarization of the VCSEL are simultaneously obtained. We obtain very simple analytical expressions that describe both linear polarizations. We show that the power of both linear polarizations depend linearly on the injected power in such a way that the total power emitted by the VCSEL is constant. We perform a linear stability analysis of this solution to characterize the region of parameters in which it can be observed. Our measurements qualitatively confirm the previous theoretical predictions.

12.
Opt Express ; 23(20): 25942-9, 2015 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-26480109

RESUMO

The humidity sensors constructed from polymer optical fiber Bragg gratings (POFBG) respond to the water content change in the fiber induced by varying environmental condition. The water content change is a diffusion process. Therefore the response time of the POFBG sensor strongly depends on the geometry and size of the fiber. In this work we investigate the use of laser micromachining of D-shaped and slotted structures to improve the response time of polymer fiber grating based humidity sensors. A significant improvement in the response time has been achieved in laser micromachined D-shaped POFBG humidity sensors. The slotted geometry allows water rapid access to the core region but this does not of itself improve response time due to the slow expansion of the bulk of the cladding. We show that by straining the slotted sensor, the expansion component can be removed resulting in the response time being determined only by the more rapid, water induced change in core refractive index. In this way the response time is reduced by a factor of 2.5.

13.
Opt Express ; 21(15): 17894-903, 2013 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-23938661

RESUMO

Time-delay signature of chaos in mutually coupled vertical-cavity surface-emitting lasers subject to polarization rotated optical injection has been investigated experimentally. Autocorrelation function and permutation entropy are used to quantitatively identify the time-delay signature of chaos. The experiment results show that the time-delay signature is sensitive to the polarization rotated angle. Minimum time-delay signature has been observed in the intermediate polarization rotated angle for the lower bias current. This is in good agreement with the theoretical prediction. At higher bias currents, the lower time-delay signature has been obtained with parallel optical injection.


Assuntos
Lasers , Modelos Teóricos , Dinâmica não Linear , Refratometria/instrumentação , Ressonância de Plasmônio de Superfície/instrumentação , Simulação por Computador , Desenho Assistido por Computador , Desenho de Equipamento , Análise de Falha de Equipamento , Luz , Espalhamento de Radiação
14.
Opt Lett ; 35(21): 3688-90, 2010 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-21042392

RESUMO

Thermal effects and dynamical hysteresis in VCSELs under dc modulation have been experimentally studied. The results show that the VCSEL turn-on and turn-off currents can display both positive hysteresis and negative hysteresis, depending on the current modulation frequency and on the substrate temperature. Numerical simulations of semiconductor laser rate equations, extended to take into account thermal effects, show a good agreement with the observations.

15.
Opt Lett ; 33(6): 587-9, 2008 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-18347718

RESUMO

We experimentally study chaos synchronization in unidirectionally coupled vertical-cavity surface-emitting semiconductor lasers (VCSELs) with polarization-preserved and polarization-selected optical injection. The measurements show, in agreement with theoretical predictions, that the maximum cross coefficient of 0.884 obtained with polarization-preserved optical injection is significantly higher than the maximum cross coefficient of 0.724 obtained with polarization-selected optical injection.

16.
J Immunol Methods ; 325(1-2): 1-8, 2007 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-17658545

RESUMO

Lpp20, an outer membrane protein of Helicobacter pylori (H. pylori), has been identified as an immunodominant antigen. To obtain mouse monoclonal antibodies (mAbs) against it and to map its antigenic epitope is potentially to develop a vaccine for prevention and treatment of H. pylori infection. In our study, the Lpp20 gene was obtained from H. pylori genomic DNA by PCR (GenBank accession no. DQ106902), cloned into pGEX-4T-1 vector and expressed in Escherichia coli (E. coli) as a recombinant fusion protein with glutathione-S-transferase (GST), which was purified by GST-affinity chromatography. mAbs were produced by the hybridoma technique using Lpp20-GST as the immunogen. Using mAb as the target molecule and immunoscreening phage-displayed random dodecapeptide library (Ph.D.-12), the positive phage clones were sequenced and analyzed. Phage clones were chosen to immunize mice to evaluate the potential of phagotopes as effective vaccines. One mimotope (SWPLYSDASGLG) showed a good match with the Lpp20 proteins at 114-117aa (DASG) and the serum of mice induced by the phage clone clearly recognized Lpp20 protein. Our work suggests that the antigenic epitope could be mapped through screening the phage-displayed peptide libraries with mAb and a mimotope of Lpp20 providing an alternative approach for the diagnosis and development of a vaccine for H. pylori.


Assuntos
Anticorpos Monoclonais/biossíntese , Antígenos de Bactérias/imunologia , Mapeamento de Epitopos , Lipoproteínas/imunologia , Biblioteca de Peptídeos , Sequência de Aminoácidos , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Afinidade de Anticorpos/imunologia , Antígenos de Bactérias/biossíntese , Antígenos de Bactérias/genética , Ligação Competitiva/imunologia , Epitopos/genética , Epitopos/imunologia , Escherichia coli/genética , Escherichia coli/metabolismo , Feminino , Imunoglobulina G/imunologia , Lipoproteínas/biossíntese , Lipoproteínas/genética , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/imunologia , Proteínas Recombinantes de Fusão/isolamento & purificação , Vacinação
17.
Nan Fang Yi Ke Da Xue Xue Bao ; 26(4): 425-7, 2006 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-16624743

RESUMO

OBJECTIVE: To prepare and identify monoclonal antibodies (mAbs) against Helicobacter pylori (Hp). METHODS: BALB/c mice were immunized with the supernatant and precipitation of cultured Hp after ultrasonication and mAbs were obtained by means of hybridoma technique. The resultant mAbs was evaluated for subtype, titer, affinity, and further identified with Lpp20, HspA, urease A, CagA, urease B, and catalase prepared by recombinant expression. RESULTS: Totally 34 hybridoma cell lines were established which secreted specific mAbs, including 31 against the supernatant and 3 against the precipitation of Hp, and the prepared mAbs showed specific reaction against Lpp20 (3 strains), HspA (2 strains), urease A (4 strains), CagA (1 strain), urease B (5 strains), and catalase (2 strains) antigens, respectively. The mAbs was all identified as immunoglobulin G1 (IgG1) and theirs titer in the culture supernatant and ascites was 1:16 to 1:32 and 1:32000 to 1:64000 respectively with affinity constants (K(aff)) ranging from 1 x 10(-10) to 5.2 x 10(-12) mol/L. CONCLUSION: The mAbs specially against Hp have been obtained, which may facilitate further study of detection and vaccine development of Hp.


Assuntos
Anticorpos Antibacterianos/biossíntese , Anticorpos Monoclonais/biossíntese , Helicobacter pylori/imunologia , Animais , Anticorpos Antibacterianos/imunologia , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Feminino , Humanos , Hibridomas/imunologia , Camundongos , Camundongos Endogâmicos BALB C
18.
Opt Lett ; 31(6): 748-50, 2006 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-16544611

RESUMO

We study the role of the bias current sweep rate in measurements of polarization switching (PS) of vertical-cavity surface-emitting lasers (VCSELs). We show that the polarization-resolved L-I (light-intensity) curve depends on the current sweep rate. As the current sweep rate increases, the PS occurs at higher bias currents for upward scans and at lower bias currents for downward scans. We also show that the delay of the dynamical bifurcation follows a power law relationship with the frequency of the ramp, in good agreement with recent theoretical predictions.

19.
Proteomics ; 6(2): 438-48, 2006 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16419015

RESUMO

Construction of a monoclonal antibody (mAb) bank containing a vast variety of antibodies against human tissue proteins is important for proteomic research. A novel strategy of subtractive immunization using fractionated native proteins was developed for high throughput generation of mAb against human plasma proteins. By this novel approach, the bottleneck of antigen preparation can be overcome by combining repeated immunization of animals with subtracted fractions of plasma or tissue proteins and identification of target antigen by immunoprecipitation/mass spectrum strategies. Plasma freshly collected from healthy adults was pooled and three fractions were prepared by size exclusion chromatography. Mice were immunized with the fractionated plasma proteins, and 205 strains of hybridomas secreting mAb were obtained after two-round subtractive immunizations and cell fusions. In the first round, 110 strains of hybridomas were established, in which 77 strains secreting mAb were identified against 10 human plasma high-abundant proteins. In the second round, plasma fraction I was absorbed with mAb against IgM, IgG, ceruloplasmin and haptoglobin. The absorbed fraction I was used as immunogen for the second round immunization and cell fusion. Ninety-five strains of hybridomas secreting mAb were obtained. Although the target antigens of mAb from 82 strains of hybridomas were identified as IgM, IgA, alpha2-macroglobulin and fibrinogen, about 85% antibodies obtained from this round were identified as new antibodies when compared with mAb obtained in the first round immunization with plasma fraction I. The results suggest that subtractive immunization with fractionated plasma proteins followed by identification of antigens with immunoprecipitation/mass spectrum may be an effective approach for rapid preparation of mAb against high-and medium-abundant plasma or tissue proteins.


Assuntos
Anticorpos Monoclonais/análise , Proteínas Sanguíneas/imunologia , Hibridomas/imunologia , Sequência de Aminoácidos , Animais , Complexo Antígeno-Anticorpo , Ensaio de Imunoadsorção Enzimática , Humanos , Imunização , Imunoprecipitação , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Fragmentos de Peptídeos/análise , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
20.
Di Yi Jun Yi Da Xue Xue Bao ; 25(9): 1169-71, 2005 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16174591

RESUMO

OBJECTIVE: To prepare and characterize the monoclonal antibodies (mAbs) against erythrocyte-binding antigen 175 of Plasmodium falciparum (EBA-175). METHODS: BALB/ c mice were immunized with purified recombinant EBA-175 and mAbs against EBA-175 were prepared by means of hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) and Western blotting were employed for characterization of the mAbs. RESULTS: Six McAbs against EBA-175 antigen were obtained, 5 of which were identified as IgG1 and one as IgG2a. The titer of these aAbs was 1:12,800 to 1:25,600 in the ascites and 1:256 to 1:512 in supernatant, and ELISA demonstrated specific binding of the 4 mAbs (1F3, 2H5, 4A1 and 4H9)with Plasmodium falciparum. Three of these mAbs recognized the protein of EBA-175 as shown by Western blotting. CONCLUSION: Six hybridoma cell lines secreting the mAbs against EBA175 with high specificity are successfully established.


Assuntos
Anticorpos Monoclonais/biossíntese , Anticorpos Antiprotozoários/biossíntese , Antígenos de Protozoários/imunologia , Plasmodium falciparum/imunologia , Proteínas de Protozoários/imunologia , Animais , Eritrócitos/imunologia
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