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Talanta ; 212: 120782, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32113545


Development of optical nanobiosensors has emerged as one of the most important bioresearch areas of interest over the past decades especially in the modern innovations in the design and utilization of sensing platforms. The application of nanobiosensors has been accelerated with the introduction of plasmonic NPs, which overcome the most of the limitations in the case of conventional optical nanobiosensors. Since the plasmonic AuNPs-based nanobiosensors provide high potential achievements to develop promising platforms in fully integrated multiplex assays, some well-developed investigations are clearly required to improve the current technologies and integration of multiple signal inputs. Therefore, in this literature, we summarized the performance and achievements of optical nanobiosensors according to plasmonic rules of AuNPs, including SPR, LSPR, SERS and chiroptical phenomena. Also, we investigated the effects of the physicochemical properties of AuNPs such as size, shape, composition, and assembly on the plasmonic signal propagation in AuNPs-based nanobiosensors. Moreover, we presented an overview on the current state of plasmonic AuNPs-based nanobiosensors in the biomedical activities. Besides, this paper looks at the current and future challenges and opportunities of ongoing efforts to achieve the potential applications of AuNPs-based optical plasmonic nanobiosensors in integration with other nanomaterials. Taken together, the main focus of this paper is to provide some applicable information to develop current methodologies in fabrication of potential AuNPs-based nanobiosensors for detection of a wide range of analytes.

Mater Sci Eng C Mater Biol Appl ; 108: 110422, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31924012


The utilization of AuNPs in therapeutic applications has been accelerated by discovering their catalytic activity consistent with the activity of natural enzymes. However, to reduce unwanted activities, it is imperative to fully understand their catalytic mechanisms to increase efficiency and safety. Therefore, along with other reports, we aimed to classify the enzymatic activity of Au nanozymes based on recent advance in their applications in biosensing and therapeutic activities. The results of the reported experiments indicate that the Au nanozymes can be used in biosensing of a wide range of agents such as molecule (H2O2 and glucose), ions, nucleic acids, proteins, cells, and pathogens. Furthermore, they can be used as potential candidates in inhibition of neurodegenerative diseases, cancer therapy, and antibacterial activities. Biosensing and therapeutic activities are generally based on colorimetric assays and the controlling the ROS level in the targeted cells, respectively. Finally, a brief explanation of the current challenges of the Au nanozymes in biomedical approaches was discussed. Indeed, this review holds a great promise in understanding the Au nanozymes properties and their development in biotechnology, medicine, and related industries.

Técnicas Biossensoriais/métodos , Ouro/química , Colorimetria , Nanopartículas Metálicas/química , Nanoestruturas/química , Espécies Reativas de Oxigênio/metabolismo
Int J Biol Macromol ; 143: 665-676, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31830450


Nanoparticles (NPs) have been widely used for immobilization of wide ranges of enzymes. However, the stabilization of enzymes on NPs is a major challenge, crucial for regulating enzymatic activity and their medical applications. To overcome these challenges, it is necessary to explore how enzymes attach to nanomaterials and their properties are affected by such interactions. In this review we present an overview on the different strategies of the enzyme immobilization into the NPs and their corresponding stability against temperature and pH. The effects of surface charge, particle size, morphology, and aggregation of NPs on the stability of immobilized enzymes were summarized. The activity of immobilized enzyme into the NPs was reviewed to disclose more detail regarding the interaction of biomolecules with NPs. The combination of enzyme immobilization with prodrugs was also reviewed as a promising approach for biomedical application of enzyme in cancer therapy. Finally, the current challenges and future applications of NPs in enzyme immobilization and the utilization of immobilized enzyme toward prodrug activation in cytoplasm of cancer cells were presented. In conclusion, this review may pave the way for providing a perspective on development to the industrial and clinical translation of immobilized enzymes.

Enzimas Imobilizadas/metabolismo , Nanoestruturas/química , Neoplasias/tratamento farmacológico , Pró-Fármacos/uso terapêutico , Animais , Estabilidade Enzimática , Humanos , Nanoestruturas/ultraestrutura
J Control Release ; 299: 121-137, 2019 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-30763621


With the development of the field of nanomedicine, the application of nanomaterials/NPs in cancer treatment has raised questions about their potential effects as well as thier unpredictable adverse effects. To date, the cytotoxic effects of nanomaterials have been investigated based on cell survival and cellular functionality, such as membrane integrity, mitochondrial activity and cell morphology. It is increasingly noted that more detailed analysis of RCD triggered by nanomaterials is essential to understand their full mechanism of action. One the one hand, this knowledge helps us to design safe therapeutics and also increases the therapeutic potential of NP-based anti-cancer drugs. The most common pathways of RCD in cancer cells include apoptosis, necrosis, necroptosis and autophagy with the latter two often act as secondary death pathways in cancer cells when the apoptotic and necrotic pathways are non-functional. This article reviews the recent developments and future perspectives in the ability of nanomaterials/NPs to induce the above forms of RCD especially necroptosis.

Nanomedicina/métodos , Nanoestruturas/uso terapêutico , Necroptose , Neoplasias/terapia , Animais , Morte Celular/efeitos dos fármacos , Humanos , Nanoestruturas/química , Nanoestruturas/ultraestrutura , Necroptose/efeitos dos fármacos
Int J Biol Macromol ; 127: 330-339, 2019 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-30654034


Herein, the thermodynamic parameters of tau upon interaction with NiO NPs were determined by fluorescence spectroscopy. Also, molecular docking studies were run to explore the binding affinities of NiO NPs clusters with different sizes of 30 Šand 50 Štoward tau. Also, cytotoxic activity of NiO NPs against SH-SY5Y was determined by MTT, LDH, caspase-9/3 activity, and expression of apoptotic Bax and Bcl-2 genes assays. DLS study showed that NiO solution had a good colloidal stability. Fluorescence study revealed that KSV values were 2.95 ±â€¯0.35 × 104, 3.31 ±â€¯0.59 × 104 and 3.92 ±â€¯0.65 × 104 at 298 K, 310 K and 315 K, respectively. Also, ∆G° (kJ/mol), ∆H° (kJ/mol) and T∆S° (kJ/mol) values were - 13.27 ±â€¯1.57, 1.98 ±â€¯0.14, 15.25 ±â€¯2.01, respectively at 298 K. Theoretical studies depicted that affinity of 5O3T segment toward NiO NP (30 Å) is higher than NiO NP (50 Å) and the proportion of Lys residues are higher in the docked pose of NiO NP (30 Å)/5O3T complex than NP (50 Å)/5O3T complex. Moreover, NiO NPs demonstrated a significant increase in the mortality of SH-SY5Y cells in an apoptotic manner. This study determined that NiO NPs may mediate the formation of electrostatic interactions with tau and induction of undesired harmful effects on neurons.

Apoptose/efeitos dos fármacos , Nanopartículas/química , Neurônios/metabolismo , Proteínas tau/metabolismo , Animais , Linhagem Celular , Neurônios/patologia , Níquel/química , Níquel/farmacologia , Ratos
Int J Biol Macromol ; 125: 778-784, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30528999


Nickel oxide nanoparticles (NiO NPs) have been used in the biological and medical sciences. However, their toxic effects against biological systems such as nervous system have not been well studied. Therefore, the adverse effect of NiO NPs on tau structure was investigated by fluorescence and CD spectroscopic methods as well as TEM study. Also, molecular dynamic study was run to extend the experimental data. Cytotoxic activity of NiO NPs against SH-SY5Y cell was determined by trypan blue exclusion, cell morphology, ROS, and apoptosis assays. ANS, Nile red, ThT assays and electron micrograph investigation revealed that NiO NPs can increase the hydrophobic portions of tau and induce the formation of amorphous tau aggregates. Far and near CD spectroscopic methods revealed that NiO NPs can change the secondary and tertiary structure of tau, respectively. Theoretical studies depicted that NiO NPs lead to folding of tau structure. In the cellular view, NiO NPs induced significant mortality and morphological effects against SH-SY5Y cells. NiO NPs also provided a significant impact on generating intracellular ROS and apoptosis induction. This study determined that NiO NPs could mediate the induction of some undesired effects on the nervous system.

Nanopartículas Metálicas/administração & dosagem , Neurônios/efeitos dos fármacos , Níquel/metabolismo , Proteínas tau/metabolismo , Apoptose/efeitos dos fármacos , Linhagem Celular , Humanos , Simulação de Dinâmica Molecular , Espécies Reativas de Oxigênio/metabolismo