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1.
Int J Neurosci ; : 1-7, 2021 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-33657959

RESUMO

AIM: In the present study we aimed to investigate the effects of nano-curcumin supplementation on gene expression and serum levels of IL-4 and TGF-ß in migraine patients. METHODS: Forty participants with episodic migraine were randomly allocated to receive 80 mg nano-curcumin (n = 20) or placebo (n = 20) in a randomized double-blind clinical trial for two months. At the beginning and the end of the study, the interictal serum levels and gene expression of IL-4 and TGF-ß in peripheral blood mononuclear cells (PBMCs) isolated from migraine patients were measured, using ELISA and real-time PCR methods, respectively. RESULTS: Intra-group assays showed a significant rise in the gene expression of both IL-4 and TGF-ß (p < 0.05) in nano-curcumin group after two months of treatment, however the serum levels were only significantly changed for IL-4 (p < 0.05). On the contrast, inter-group assays revealed no statistical differences between nano-curcumin and placebo group in terms of IL-4 and TGF-ß gene expression, while the serum levels of IL-4 was observed to be increased significantly (p = 0.03) following two month nano-curcumin supplementation. CONCLUSION: The findings of the present trial suggest that the treatment with nano-curcumin could induce significant levels of IL-4, in favour of anti-inflammatory effects, while has a minimal effects on the both gene expression and serum levels of TGF-ß. Further studies are required to determine the exact mechanism of action of curcumin in patients with migraine.

2.
J Neurovirol ; 2021 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-33651323

RESUMO

Human T-cell lymphotropic virus type 1 (HTLV-1) is the first isolated retrovirus from humans, and 2-3% of infected individuals suffer from HTLV-1 associated myelopathy tropical spastic paraparesis (HAM-TSP). Previous studies indicated that the risk of HAM-TSP could be correlated with the individuals' genetic alterations. Mashhad is one of the areas infected with HTLV-1 in Iran. This study designed to examine the association between several important gene polymorphisms and HAM-TSP. Genotypes of 232 samples from controls, HTLV-1 carriers, and HAM-TSP patients were examined for FAS-670 (A > G), CXCL10-1447 (A > G), Foxp3-3279 (C > A), IL-18 -137 (C > G), and IL-18 -607 (C > A) gene polymorphisms by different polymerase chain reaction (PCR) techniques. A non-significant association was observed between FAS-670 A > G, Foxp3-3279 C > A, and IL-18 -137 C > G gene polymorphisms and HAM-TSP. Nevertheless, a significant (P < 0.001) association between CXCL10-1447 A > G and IL-18 -607 C > A gene polymorphisms with HAM-TSP was observed in our study population. As previous studies revealed that the CXCL10 level in the cerebrospinal fluid of HAM-TSP patients was associated with the disease progression, and as we noticed, a direct association was observed between CXCL10-1447 A > G polymorphism and HAM-TSP. These polymorphisms might be recommended as a valuable prediction criterion for the severity of the disease. The contradiction between our findings and other studies regarding IL-18 -607 C > A gene polymorphism might be associated with various factors such as genotypes frequency in diverse races and population heterogeneity in the city of Mashhad.

3.
Transplant Proc ; 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-33272649

RESUMO

INTRODUCTION: Molecular testing such as nasopharyngeal viral polymerase chain reaction (PCR) (NVP) is available now in most hospitals and widely used to identify respiratory viral infections (RVIs) in solid organ transplantation (SOT) recipients. MATERIALS AND METHODS: A retrospective multicenter study at 8 hospitals from March 1, 2016, to April 30, 2019. We included all adult SOT recipients who were admitted to the hospitals and had their first NVP post transplantation. RESULTS: A total of 102 adult SOT recipients were enrolled. NVP test was positive in 33 (32.4%) SOT recipients and negative in 69 (67.6%). Median age was more than 60 years old with female predominance in both groups. The majority of patients who had positive NVP were hospitalized either in fall or winter seasons (91%). RVI symptoms were documented in about 73% of the positive NVP group. Rhinovirus was the most common identified virus (48.4%). On logistic regression analysis, clinical presentation in fall or winter seasons, presenting with upper respiratory infection (URI) symptoms and taking prednisone ≥10 mg/d were significantly associated with positive NVP. This model classified patients into 3 categories of risk for RVIs-low (none of the variables), 0%; intermediate (1 variable), 6.5%; and high (≥2 variables), 55.4% with P < .001 for all predictors. CONCLUSION: SOT recipients who are taking prednisone (≥10 mg) and have URI symptoms in fall or winter seasons are more likely to have RVIs.

4.
Comput Intell Neurosci ; 2020: 8811630, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33178258

RESUMO

In this study, an essential application of remote sensing using deep learning functionality is presented. Gaofen-1 satellite mission, developed by the China National Space Administration (CNSA) for the civilian high-definition Earth observation satellite program, provides near-real-time observations for geographical mapping, environment surveying, and climate change monitoring. Cloud and cloud shadow segmentation are a crucial element to enable automatic near-real-time processing of Gaofen-1 images, and therefore, their performances must be accurately validated. In this paper, a robust multiscale segmentation method based on deep learning is proposed to improve the efficiency and effectiveness of cloud and cloud shadow segmentation from Gaofen-1 images. The proposed method first implements feature map based on the spectral-spatial features from residual convolutional layers and the cloud/cloud shadow footprints extraction based on a novel loss function to generate the final footprints. The experimental results using Gaofen-1 images demonstrate the more reasonable accuracy and efficient computational cost achievement of the proposed method compared to the cloud and cloud shadow segmentation performance of two existing state-of-the-art methods.

5.
Complement Ther Clin Pract ; 41: 101256, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33147541

RESUMO

BACKGROUND: The present study was aimed to evaluate the nano-curcumin supplementation on Th1/Th17 balance by assessment of gene expression and serum level of interferon gamma (IFN-γ) and interleukin-17 (IL-17) in migraine patients. METHODS: Forty participants with episodic migraine were randomly allocated to receive 80 mg nano-curcumin (n = 20) or placebo (n = 20) in a randomized double-blind clinical trial for two months. The expression of IFN-γ and IL-17 from peripheral blood mononuclear cells and IFN-γ and IL-17 serum levels were measured, using a real-time PCR and ELISA methods, respectively. RESULTS: Compared to placebo group, two month nano-curcumin supplementation led to a significant reduction in serum levels and expression of IL-17 mRNA (P = 0.006 & 0.04, respectively), while there was no statistical difference regarding serum levels and expression of IFN-γ mRNA. CONCLUSION: Nano-curcumin supplementation in migraine patients led to a significant reduction in gene expression and plasma levels of IL-17 compared to control group.

6.
Zootaxa ; 4852(3): zootaxa.4852.3.1, 2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-33056411

RESUMO

A faunal and molecular taxonomic study of Iranian bark and ambrosia beetle species based on field collections, museum specimens and literature data was carried out from in the period 2011-2016. A total of 29 genera and 84 species were found for Iran. A morphological key for species identification is provided. Molecular identification based on mitochondrial cytochrome c oxidase I (CO1) barcoding region gene was also performed for the collected specimens to confirm morphological identification, and an exclusive DNA barcode was provided and registered for the samples collected in this study. Host plants and distribution of each species in the Palearctic region and in Iran are reported in the key.


Assuntos
Ambrosia , Besouros , Gorgulhos , Animais , Irã (Geográfico) , Casca de Planta
7.
Zootaxa ; 4786(4): zootaxa.4786.4.9, 2020 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-33056466

RESUMO

The materials on the genus Vertagopus Börner from the Caucasus and Iran are revised. Two new species, V. persicus sp. nov. and V. nunataki sp. nov., are described and fauna of the genus in the Western Asia is commented.


Assuntos
Artrópodes , Distribuição Animal , Animais , Irã (Geográfico)
8.
Biol Trace Elem Res ; 2020 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-32385715

RESUMO

In this study, we aimed to systematically review the literature to evaluate the effects of magnesium (Mg) supplementation on blood pressure (BP) and obesity measure among patients with type 2 diabetes mellitus (T2DM). Major electronic databases of Web of Science, the Cochrane library, PubMed, and Scopus were searched completely from the inception until 15 October 2019 to identify randomized clinical trials (RCTs) pertaining to the topic of interest. All outcomes were pooled using a random-effects model and expressed as weighted mean differences (WMD) with 95% confidential intervals (CI). Heterogeneity, sensitivity analysis, and publication bias were also assessed using standard methods. The pooled analysis of five RCTs showed that Mg supplementation did not affect body weight (WMD: - 0.01 kg, 95% CI: - 0.36 to 0.33), BMI (WMD: - 0.07, 95% CI: - 0.18 to 0.04), and waist circumference (WMD: 0.12, 95% CI: - 1.24 to 1.48) in T2DM patients compared to the control groups of the patients who received placebo. However, pooling seven RCTs together showed significant reduction of systolic blood pressure (WMD: - 5.78 mmHg, 95% CI: - 11.37 to - 0.19) and diastolic blood pressure (WMD: - 2.50 mmHg, 95% CI: - 4.58 to - 0.41) in T2DM patients. Furthermore, subgroup analysis by dose of intervention, intervention duration, and type of intervention suggested that Mg supplementation for > 12 weeks, in doses higher than 300 mg/day or inorganic forms, could significantly decrease both systolic and diastolic BP in T2DM patients. Based on the findings, Mg supplementation has beneficial effects on BP in type 2 diabetes patients independent of body weight status. However, further investigations are needed to provide more reliable evidences.

9.
Iran J Allergy Asthma Immunol ; 19(1): 18-26, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32245317

RESUMO

Substance P (SP) is a neurotransmitter emitted from neurons that plays a role in the pathogenesis of itching conditions including chronic urticarial (CU). The present research aims to investigate the serum level of S.P among CU patients and compare them with healthy subjects and explore how it correlates with the severity of urticaria. The present research was conducted on 87 CU patients who visited the allergy clinic of Ghaem Hospital, Mashhad, Iran from October 2017 to June 2018. Besides, 86 healthy subjects were recruited as the control group. Background information of patient was collected including age, sex, duration of the disease and the co-occurrence of angioedema. S.P serum level was measured in two groups by ELISA method. In the patients group, the autologous serum skin test (ASST) was performed along with the urticaria evaluation questionnaire include Urticaria Activity Score 7 (UAS7), Urticaria Control Test (UCT) and Chronic Urticaria Quality of Life (CU-Q2OL). Among the patients, the SP serum level showed to be about two times higher than the healthy subjects (p˂0.001). SP showed to be increased as patients' age grew (p=0.010). In patients with a positive ASST, SP level was higher (p=0.012). No correlation was found between SP and the presence of angioedema among patients. There was no correlation between the SP serum level and the scores obtained from urticaria evaluation questionnaires. SP among CU patients was higher than healthy subjects. SP was also higher among female, older and positive ASST patients. The SP value was not correlated with the severity of urticaria, angioedema. In conclusion, Using SP antagonist drugs could be a potential treatment for chronic urticaria.


Assuntos
Urticária Crônica/sangue , Urticária Crônica/patologia , Substância P/sangue , Adulto , Estudos Transversais , Feminino , Humanos , Masculino
11.
Pak J Biol Sci ; 23(2): 173-180, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31944077

RESUMO

BACKGROUND AND OBJECTIVE: In spite of therapeutic effect of tamoxifen on the breast cancer, it has some side effects on the liver including non-alcoholic fatty liver disease. In this study the effects of Rosa canina distilled water on the tamoxifen-induced fatty liver and oxidative stress status in male rats were investigated. MATERIALS AND METHODS: Twenty four adult male Wistar rats were randomly divided into 4 groups of 6: 1st group: Untreated control rats (C), 2nd group (T): The rats received tamoxifen, 3rd group (T+R): Rats received tamoxifen and Rosa canina distilled water and 4th group (R): Rats received only Rosa canina distilled water. Tamoxifen at 1 mg kg-1/day was injected subcutaneously for 7 days and the rats received orally Rosa canina distilled water at 1 mL/rat/daily for 14 days. At the end of the study, animals were studied for serum biochemical parameters (glucose, lipid profile, BUN, creatinine, uric acid, urea, ALT, AST, ALP, total protein, bilirubin, oxidative stress indices, sperm analysis and histology of the liver. The data were analyzed with SPSS software version 20 and expressed as Mean±SD. RESULTS: Rosa canina distilled water improved liver enzyme and renal function indices which disturbed due to tamoxifen treatment. While tamoxifen enhanced lipid peroxidation, Rosa canina distilled water reduced it. In addition, tamoxifen reduced the mobility, morphology and viability of sperms, but the Rosa canina distilled water enhanced the sperm parameters. Histological results also confirmed the adverse effect of tamoxifen and the favorable impact of the Rosa canina distilled water on the liver structures of animals. CONCLUSION: Rosa canina distilled water could modulate tamoxifen-induced fatty liver as well as improving the sperm parameters.


Assuntos
Fígado Gorduroso/terapia , Extratos Vegetais/farmacologia , Rosa/química , Tamoxifeno/efeitos adversos , Água/química , Animais , Antioxidantes/farmacologia , Glicemia/análise , Fígado Gorduroso/induzido quimicamente , Rim/efeitos dos fármacos , Peroxidação de Lipídeos , Masculino , Estresse Oxidativo , Plantas Medicinais , Ratos , Ratos Wistar , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Ureia/farmacologia
12.
Iran J Basic Med Sci ; 22(9): 989-996, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31807241

RESUMO

Objectives: Allergic rhinitis (AR) is a common hypersensitivity disease worldwide. Immunotherapy has been performed as the best treatment for years. This study aimed to study the gene expression pattern of immune system cells following an accelerated rush immunotherapy protocol (ARIT) in patients with AR. Materials and Methods: Fifteen patients with AR (15-55 years old) resident in Mashhad, Iran, with positive prick test to regional aeroallergens (weed mix, grass mix, tree mix, and Salsola) enrolled in this study. All patients were treated for three months with 3-day ARIT protocol between July 2015 and August 2016. Clinical symptoms and quality of life were recorded by two questioners. The expression levels of FOXP3, TGF-ß, IL-10, IL-17, IL-4, and IFN-γ genes in patient's peripheral blood mononuclear cells were evaluated by SYBR Green real-time RT-PCR technique. Results: The severity of disease and quality of life showed significant improvement following ARIT (P-value<0.05). Gene expression of IFN-γ and IL-10 was increased whereas TGF-ß and IL-4 down-regulated, following ARIT, but these changes were not significant. However, gene expression of FOXP3 and IL-17 was significantly increased after intervention when compared with the baseline (P-value< 0.002). Conclusion: Significant up-regulation of FOXP3 and IL-17 genes, additionally, a significant improvement in the clinical signs following ARIT might be related to increases in HLA-DR- and FOXP3+ Treg population at the initiation phase of ARIT. Employing the flow cytometry technique to study the phenotype of these cells is suggested for future studies.

13.
Rep Biochem Mol Biol ; 8(2): 111-118, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31832433

RESUMO

Background: Allergic Rhinitis (AR) is an IgE-mediated inflammatory disorder with high morbidity rates. The eitiology of this disease is understood to occur from a complex interaction between genetic and environmental factors. T helper type 2 cells have been shown to have a crucial role in atopic disease due to their production of the cytokines, intelukin (IL)-13 and IL-4, involved in inflammation. Research has shown single nucleotide polymorphisms (SNP) of the IL-13 and IL-4 genes to be associated increased levels of IgE and with allergic diseases such as, allergic rhinitis, asthma, and atopic dermatitis. Specifically, the rs2243250 SNP of IL-4 and the rs20541 SNP of IL-13 have been shown to be associated with AR. Methods: A case-control study was designed to investigate the relationship between the two SNPs rs2243250 and rs20541 with the incidence of AR. The SNPs were examined in patients with AR and healthy controls (86 patients and 86 controls). Blood samples were collected and DNA was extracted to evaluate the SNPs by RFLP-PCR. Results: Recessive analysis model of the IL-13 gene (GG vs. AA+AG) revealed that the GG genotype was more common in AR patients (P=0.36) )OR=0.8 [81% CI 0.38-1.6]). For the IL-4 gene (TC vs. TT+CC), the TC genotype was more common in AR patients (P = 0.0022)) OR=0.71 [60% CI 1.41-5.02]). Furthermore, in the IL-4 gene, the 590 T>C polymorphism had a significant association with AR. However, no association was found between AR and the IL-13 rs20541 polymorphism. Conclusion: Our findings suggest that the IL-13 polymorphism (rs20541, Exo 4, G>A, Arg130Gln) and IL-4 polymorphism (rs2243250= C-590T, promoter, T>C) are co-associated with AR and sensitivity to aeroallergens. However, this study used a cohort of AR patients and healthy controls from the northeast of Iran. Given the influence of ethnicity and environment on genetics, further investigation is needed to elucidate the role of SNPs in IL-4 and IL-13 in AR among different populations.

14.
Zootaxa ; 4563(2): zootaxa.4563.2.8, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-31716546

RESUMO

A new species, Phytocoris (Compsocerocoris) darakiensis sp. nov. is described from Kurdistan province, Iran. A taxonomic key to the species of amygdali-group of the subgenus Compsocerocoris Reuter known in Iran, male and female habitus photographs and illustrations of male genitalia of the new taxon are provided. Diagnosis of the new species is based on a comparison with other congeneric found from Iran. The type specimens were deposited in the insect collection of the University of Guilan, Rasht, Iran.


Assuntos
Hemípteros , Heterópteros , Distribuição Animal , Animais , Feminino , Genitália Masculina , Irã (Geográfico) , Masculino
15.
Zootaxa ; 4648(1): zootaxa.4648.1.6, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31716962

RESUMO

Two new species, Phytocoris (Compsocerocoris) amardus sp. nov. and Phytocoris (Compsocerocoris) hyrcaniaensis sp. nov. are described from Guilan province, Iran. Description, diagnoses, male color habitus image and illustrations of male genitalia are presented for each taxon to aid in unequivocal identification. A revised taxonomic key to the species of subgenus Compsocerocoris Reuter known in Iran is also provided. The type specimens were deposited in the insect collection of the University of Guilan, Rasht, Iran.


Assuntos
Hemípteros , Heterópteros , Distribuição Animal , Animais , Cor , Genitália Masculina , Irã (Geográfico) , Masculino
16.
Allergy Asthma Immunol Res ; 11(6): 885-893, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31552722

RESUMO

Immunotherapy is the standard of treatment for long-life relief of symptoms of allergic rhinitis. Vitamin D may affect the outcomes of treatment. This study evaluated the clinical efficacy of subcutaneous allergen immunotherapy in adult patients with allergic rhinitis based on the serum level of vitamin D. Patients with persistent allergic rhinitis and positivity for skin prick test were evaluated by Sino-nasal Outcome Test (SNOT-22) and Mini Rhinoconjunctivitis Quality of Life Questionnaire (MiniRQLQ) before subcutaneous allergen immunotherapy and during the maintenance phase to assess the relation of the serum level of vitamin D and the clinical efficacy of immunotherapy. After immunotherapy, the greatest reduction in SNOT-22 scores were reported in patients with vitamin D sufficiency (39.0 ± 9.2), followed by vitamin D suboptimal provision (35.1 ± 12.1), insufficiency (25.0 ± 7.5), and deficiency (18.3 ± 6.0) (P < 0.001). The MiniRQLQ reduction in patients with vitamin D sufficiency, suboptimal provision, insufficiency, or deficiency was 30.7 ± 8.7, 27.1 ± 8.7, 20.0 ± 8.6, or 17.4 ± 7.1, respectively (P < 0.001). Both of SNOT-22 and MiniRQLQ scores decreased significantly following immunotherapy in patients with different levels of vitamin D. However, these effects were more pronounced when the level of vitamin D was sufficient.

17.
Arch Iran Med ; 22(6): 344-352, 2019 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-31356102

RESUMO

The oldest literature that mentioned the immunity was related to the plague of Athens in 430 BC. The germ theory had more influence on the description of the discrimination between self and non-self which is the core concept of immunology. Allergic diseases were known from 2,000 years ago while Rhazes wrote the first medical monograph about seasonal allergic rhinitis. In Iran, Dr. Mohammad Kermanshahi introduced the basic concepts of serology in 1900. The first Department of Serology was established at Tehran University, Faculty of Medicine in 1951 by Prof. Dr. Hassan Mirdamadi. After that, immunology entered into the cellular and molecular period. Prof. Mohammad Ali Maleki established the first Iranian Society of Allergy and then Prof. Abolhassan Farhoodi found the first Department of Clinical Immunology and Allergy at Tehran University. In this paper, we review the role of famous Iranian scientists and physicians in the progress of modern immunology and allergy from a historical perspective.


Assuntos
Alergia e Imunologia/história , Médicos/história , História do Século XIX , História do Século XX , História do Século XXI , Humanos , Irã (Geográfico)
18.
Postepy Dermatol Alergol ; 36(3): 276-281, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31333344

RESUMO

Introduction: The relationship between allergic and autoimmune diseases is an important issue, which has recently attracted the researchers' interest. Aim: To determine the relationship between atopy and psoriasis. Material and methods: This case-control study was conducted on 102 patients referred to the Ghaem Hospital, Mashhad, Iran, in 2016. The participants were assigned into two groups: experimental and control groups, including the patients suffering from psoriasis and those with no history of cutaneous or other systemic diseases, respectively. Both groups filled in the ISAAC questionnaire and had skin prick tests. In addition, the serum levels of immunoglobulin E (IgE) and blood eosinophil cell count were measured. The data were analysed using the regression test through SPSS version 16. Results: According to the results of the ISAAC questionnaire, there was a significant difference between the control and experimental groups in terms of asthma (p = 0.04). The mean serum concentrations of IgE and eosinophil cell count were not significantly different between the experimental (153.93 IU/ml and 187.77 cells/µl, respectively) and control groups (152.19 IU/ml and 187.68 cells/µl, respectively) (p = 0.057 and p = 0.886, respectively). In addition, there was an indirect correlation between the eosinophil cell count and psoriasis severity (p = 0.032, r = -0.297). Furthermore, the comparison of the skin prick test results revealed no significant difference between the two groups regarding the number of positive and negative cases (p = 0.436). Conclusions: The findings suggested that atopy was not common in the patients with psoriasis and supported the concept that atopy protects against such autoimmune diseases such as psoriasis.

19.
Microb Pathog ; 134: 103578, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31175973

RESUMO

Atherosclerosis is a multifactorial life-threatening disease which an epidemiologic study in Northeastern Iran showed its association with HTLV-1 infection. Therefore, a cross-sectional study of 39 newly diagnosed subjects with angiography test in three groups including 14 coronary artery disease+HTLV-1+ (CAD+HTLV-1+), 8 CAD-HTLV-1+, and 17 CAD+HTLV-1- patients and 11 healthy subjects (CAD-HTLV-1-) were conducted. In the present study, Tax and proviral load (PVL) as HTLV-1 virulence factors, along with host chemokine receptor 1 (CCR1), and CCR2 were investigated. Real-time PCR TaqMan method was carried out for PVL measurement and HTLV-1-Tax, CCR1, and CCR2 expressions in peripheral blood mononuclear cells (PBMCs). Furthermore, the main risk factors, lipid profile, and complete blood count (CBC) were assessed. Expression of CCR1 in CAD+HTLV-1+ group was higher than CAD-HTLV-1+ (P = 0.01) and healthy subjects (P = 0.02). Expression of CCR1 in CAD+HTLV-1+ was higher in comparison with CAD+HTLV-1-group but did not meet 95% CI (P = 0.02), but meaningful at 91% CI. In addition, expression of CCR2 in CAD+HTLV-1+ subjects was higher than CAD-HTLV-1+ and CAD+HTLV-1- (P = 0.001, P = 0.005, respectively). In CAD+HTLV-1- subjects, CCR2 was higher than CAD-HTLV-1+ (P = 0.03). The mean PVL in CAD+HTLV-1+ group is more than CAD-HTLV-1+ (P = 0.041). In HTLV-1+ patients Tax had a positive correlation with cholesterol (R = 0.59, P = 0.01), LDL (R = 0.79, P = 0.004) and a negative correlation with HDL (R = -0.47, P = 0.04). These correlations were stronger in CAD+HTLV-1+. Findings showed that HTLV-1 could alter the expression of CCR2 and, less effect, on CCR1. Moreover, the strong correlation between CCR2 and HTLV-1-Tax with cholesterol, LDL and HDL showed that Tax as the main HTLV-1 virulence factor in cytokine deregulation might be had indirect effects on cholesterol, LDL, and HDL levels.


Assuntos
Doenças Cardiovasculares/etiologia , Doenças Cardiovasculares/virologia , Infecções por HTLV-I/complicações , Infecções por HTLV-I/virologia , Vírus Linfotrópico T Tipo 1 Humano/patogenicidade , Idoso , Aterosclerose/epidemiologia , Aterosclerose/virologia , Colesterol/sangue , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Doença da Artéria Coronariana/etiologia , Doença da Artéria Coronariana/virologia , Estudos Transversais , Citocinas/sangue , Feminino , Infecções por HTLV-I/epidemiologia , Humanos , Irã (Geográfico) , Leucócitos Mononucleares/virologia , Masculino , Pessoa de Meia-Idade , Provírus , Reação em Cadeia da Polimerase em Tempo Real , Receptores CCR1/sangue , Receptores CCR1/metabolismo , Receptores CCR2/sangue , Receptores CCR2/metabolismo , Fatores de Risco , Carga Viral , Fatores de Virulência
20.
Clin Immunol ; 205: 125-129, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31152891

RESUMO

Nasal polyps (NP) are associated with inflamed mucosa of unknown etiology. The role of T cells in nasal polyposis is unclear. Invariant natural killer T cells (iNKT) can promote Th2 responses and have been implicated in some types of asthma. As there are shared inflammatory pathways involved in asthma and NPs, we evaluated the frequency of iNKT in 17 patients with NPs, but without asthma. A median of 6% polyp cells were T lymphocytes, of which iNKT were 0 to 2.38% (mean 0.674%). In the matched group (n = 10), iNKT in NPs was significantly higher than PBMCs (1.057% vs 0.155%, P < 0.05). Relative expression of Vα24 to TCR-beta genes in polyps (n = 14) was higher than blood in matched samples (n = 4). The presence of greater proportions of iNKT in NPs than in blood suggests that iNKT may play a role in the pathogenesis of nasal polyposis.


Assuntos
Pólipos Nasais/patologia , Células T Matadoras Naturais/patologia , Adolescente , Adulto , Doença Crônica , Feminino , Genes Codificadores da Cadeia beta de Receptores de Linfócitos T/genética , Humanos , Imunoglobulina E/metabolismo , Contagem de Leucócitos , Leucócitos Mononucleares , Masculino , Pessoa de Meia-Idade , Pólipos Nasais/genética , Pólipos Nasais/metabolismo , Pólipos Nasais/cirurgia , Receptores de Antígenos de Linfócitos T/genética , Rinite/genética , Rinite/metabolismo , Rinite/patologia , Sinusite/genética , Sinusite/metabolismo , Sinusite/patologia , Adulto Jovem
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