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1.
Dalton Trans ; 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32432591

RESUMO

Plasmon-mediated photocatalytic systems often suffer from weak absorption spectra overlap which limits energy transfer between plasmon metals and semiconductors. Herein, Au-Agx@CdS90 nanoparticles (NPs) with adjustable spectral overlap were prepared. Au-Ag hollow nanoparticles (HNPs) with tunable plasmon absorption peaks were used as the template and were coated with CdS to achieve stepwise spectral overlap for enhanced energy transfer. As the spectral overlap increased between Au-Ag HNPs and CdS, the H2 evolution rate increased and then decreased. Under visible-light irradiation, Au-Ag487@CdS90 nanoparticles (NPs) delivered an H2 evolution rate of 18.73 mmol h-1 g-1, which was 2.2 times higher than pure CdS. The plasmon resonance energy transfer from Au-Ag HNPs to the CdS semiconductor increased the generation of charge carriers in the semiconductor and enhanced the photocatalytic performance. By regulating the position of the plasmon absorption peaks of the noble metal nanoparticles, changing the spectral overlap between metal and semiconductor to enhance the PRET effect is beneficial to the design of new plasmon photocatalysts.

2.
Dalton Trans ; 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32363368

RESUMO

Designing low-cost and high-performance bifunctional electrocatalysts towards hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is vitally important for water splitting. Herein, we synthesize Co2-xNixO2 (0 < x < 1.0) hexagonal nanosheets with different Co/Ni molar ratios via a facile coprecipitation process followed by calcination under an Ar atmosphere. Changing the Co/Ni molar ratios of the Co2-xNixO2 products is found to have a momentous influence on the microstructures, specific surface areas and electrocatalytic performances. At a Co/Ni molar ratio of 0.6, the Co1.4Ni0.6O2 nanosheet exhibits the largest specific surface area of 60.63 m2 g-1, the best OER with an onset overpotential of 278.5 mV, and HER of 72.8 mV as a bifunctional electrocatalyst. Meanwhile, the minimum Tafel slope is 113.6 mV dec-1 for OER and 77.4 mV dec-1 for HER. The Co1.4Ni0.6O2 nanosheet has excellent OER and HER activity at 0.1 mg cm-2 trace loading. Moreover, we construct an overall water splitting cell using the Co1.4Ni0.6O2 bifunctional electrocatalyst in a two-electrode system to further demonstrate the practical application, which needs a cell voltage of 1.75 V at a current density of 10 mA cm-2 and exhibits great long-term stability. These results provide an efficient strategy for the rational design of Co-based oxides towards bifunctional overall water electrocatalysts.

3.
PLoS One ; 15(5): e0233205, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32437385

RESUMO

This study draws on previous studies to develop the Social Networking Sites Use Multi-Motive Grid Questionnaire (SNSU-MMG) and test its reliability and validity. The results show that social networking sites use motivation includes four factors: cognitive motivation, emotional motivation, leisure motivation and herding motivation. Confirmatory factor analysis, reliability and validity tests show that the questionnaire has good structural validity, internal consistency and split-half reliability. The SNSU-MMG can be used as a measurement tool for users' social networking sites use implicit motivation.

4.
Nanotechnology ; 31(17): 175102, 2020 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-31935712

RESUMO

Carbon quantum dots (CDs) have attracted increased attention in recent decades because of their various applications in biosensing, bioimaging and drug delivery. In the present study, we have synthesized bifunctional ibuprofen-based carbon quantum dots (ICDs) using a simple one-step microwave-assisted method, for simultaneous bioimaging and anti-inflammatory effects. The ICDs exhibited high stability, low toxicity, negligible cytotoxicity and good biocompatibility in water. In particular, the produced ICDs demonstrated a decent imaging ability and excellent anti-inflammatory effects in vivo, making them potentially useful in bioimaging and future clinical treatment. Our results demonstrated that ICDs show promise in applications such as multifunctional biomaterials, depending on the selection of carbon sources, which would provide important guidance for the future design of multifunctional CDs in the field of biomedicine.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31981465

RESUMO

The only feasible access to non-face-centered cubic (FCC) copper was by physical vapor deposition under high vacuum. Now, non-FCC copper is observed in a series of alkynyl-protected Cu53 nanoclusters (NCs) obtained from solution-phase synthesis. Determined by single-crystal X-ray crystallography, the structures of Cu53 (C≡CPhPh)9 (dppp)6 Cl3 (NO3 )9 and its two derivatives reveal an ABABC stacking sequence involving 41 Cu atoms. It can be regarded as a mixed FCC and HCP structure, which gives strong evidence that Cu can be arranged in non-FCC lattice at ambient conditions when proper ligands are provided. Characterization methods including X-ray absorption fine structure, XPS, ESI-MS, UV/Vis, Auger spectroscopy, and DFT calculations were carried out. CuII was shown to successively coordinate with introduced ligands and changed to CuI after bonding with phosphine. The following addition of NaBH4 and the aging step further reduced it to the Cu53 NC.

6.
Dalton Trans ; 49(3): 750-756, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31850467

RESUMO

Exploring efficient and stable oxygen evolution reaction (OER) catalysts with earth-abundant elements has been an urgent task in water splitting. Metal-organic frameworks (MOFs), possessing abundant active metal sites and tunable porous crystalline structures, are promising as OER catalysts. In this paper, a MOF based on benzotriazole-5-carboxylate (Co3-btca) that contains unsaturated coordinated metal centers and a 1D channel was selected to act as an OER catalyst. To optimize the OER performance, isostructural bimetallic and trimetallic frameworks were obtained by doping with Fe and/or Ni ions. The optimized Co2.36Fe0.19Ni0.45-btca possesses the lowest overpotential of 292 mV at 10 mA cm-2 and a small Tafel slope of 72.6 mV dec-1. The enhanced catalytic performance could be attributed to the synergistic effect between the unsaturated coordinated Co, Fe and Ni sites, which are beneficial for the nucleophilic attack of OH- forming adsorption intermediates.

7.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(6): 827-834, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-31880113

RESUMO

OBJECTIVE: To detect the effects and mechanism of asprosin (Asp) and spartin on the injury of mice cardiac microvascular endothelial cells (CMECs) induced by high glucose. METHODS: The cultured CMECs were divided into 2 groups, one group is normal group (5.5 mmol/L glucose in the medium) and another is HG group (30 mmol/L glucose in the medium). Real-time PCR (qRT-PCR) and Western blot were respectively used to detect the mRNA level of spastic paraplegia 20 (SPG20) and protein expression of spartin in CMECs. Upregulation or downregulation of the expression of spartin was achieved via transfection with adenovirus (Ad) or small interfering RNA (siRNA) respectively. CMECs with downregulation of spartin expression were firstly treated with anti-oxidant N-acetylcysteine (NAC) or Asp respectively for 48 h, and then were interfered with 30 mmol/L glucose for 24 h afterward. The apoptosis of cell was detected by flow cytometry. Nitric oxide (NO) production was detected by NO probe and ELISA kit. The intracellular reactive oxygen species (ROS) levels were tested by DHE staining and ELISA kit. Type 2 diabetic model mice were established and then divided into T2DM group and T2DM+Asp group. After the model mice were established successfully (random blood glucose was more than 16.7 mmol/L), Asp (1 µg/g) was intraperitoneally injected once a day. After 2 weeks, mice echocardiography was performed to test cardiac diastolic function. The integrity of the microvascular endothelium was observed by scanning electron microscopy. RESULTS: Compared with the normal group, the mRNA level of SPG20 and protein expression of spartin in mice CMECs of HG group were significantly reduced (P < 0.05). Under the condition of high glucose, Ad transfection induced significant decrease of the intracellular ROS level and the apoptosis level of the CMECs (P < 0.05), while NO increased after Ad transfection. In contrast, siRNA intervention resulted in opposite effect. In addition, the antioxidant NAC partly reversed the above changes caused by downregulating spartin. Asp upregulated the level of SPG20 mRNA and spartin protein expression in CMECs, reduced ROS production, reduced apoptosis and increased NO production. However, intervention effects of Asp, such as decreasing of ROS production, inhibiting apoptosis of CMECs and increasing of NO production, were partly reversed in spartin downregulated cells. In vivo, we found that Asp can improve cardiac function and increase the integrity and smoothness of cardiac microvascular endothelium in type 2 diabetic mice. CONCLUSION: Asp can inhibit oxidative stress in mice CMECs through upregulating spartin signaling pathway, thereby alleviating the damage of microvascular endothelium in diabetic heart.


Assuntos
Diabetes Mellitus Experimental , Células Endoteliais , Animais , Apoptose , Células Cultivadas , Camundongos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio
8.
Exp Appl Acarol ; 79(1): 125-135, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31396760

RESUMO

To investigate the prevalence of Anaplasma, Ehrlichia, Bartonella and Theileria, we collected ticks from small mammals in six counties of Zhejiang Province in southeastern China. Polymerase chain reaction (PCR) amplification was performed to test Anaplasma, Ehrlichia, Bartonella and Theileria in tick samples. Positive PCR products were sequenced and then compared with previously published sequences deposited in GenBank using BLAST. About 292 adult ticks were captured and the dominant tick species were Ixodes sinensis and Haemaphysalis longicornis. Overall, 34 ticks (11.6%) were tested positive for at least one pathogen of Anaplasma, Ehrlichia, Bartonella and Theileria. Rates of PCR-positivity to Anaplasma, Ehrlichia, Bartonella and Theileria were 5.5, 1.7, 2.4 and 2.4%, respectively. Positive rates of Anaplasma, Bartonella and Theileria were significantly different among ticks of different species. Prevalence of Anaplasma and Theileria varied significantly among ticks of different counties. Anaplasma, Ehrlichia, Bartonella and Theileria were widely prevalent in ticks in Zhejiang Province suggesting other tick-borne pathogens should also be suspected if patients had history of tick bites.


Assuntos
Anaplasma/fisiologia , Bartonella/fisiologia , Ehrlichia/fisiologia , Ixodidae/microbiologia , Ixodidae/parasitologia , Theileria/fisiologia , Animais , China , Feminino , Masculino , Especificidade da Espécie
9.
Addict Behav Rep ; 10: 100209, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31463357

RESUMO

Interpersonal relationship and loneliness are important factors affecting internet addictive behavior of individuals. In the present study, we investigated intimate interpersonal relationships and loneliness in internet-addicts. We recorded event-related potentials (ERPs) of 32 internet addicts and 32 non internet-addicts. Participants viewed intimate-/conflict-relationship, happy/lonely, and neutral images. Results concerning attention probes showed that the accuracy rate of attention probes of internet-addicts was significantly lower than that of non internet-addicts; whereas, there was no significant difference in the reaction time of attention probes. Moreover, the differences in the mean amplitude and latency of P1, N1, N2P3, and LPP between internet-addicts and non internet-addicts were insignificant. Then, we found that the P1 amplitude of conflict images was significantly higher than that of intimate images among non internet-addicts; whereas internet-addicts indicated an insignificant difference between the two types of images. The P1 amplitude of lonely images was significantly higher than that of happy images among internet-addicts, but non internet-addicts were insignificant. The questionnaire data also obtained similar conclusions based on the EEG data. Finally, internet-addicts reported significantly higher loneliness scores than those of non internet-addicts. These results suggested that the social cognitive function of internet-addicts was probably impaired, especially in the cognition of interpersonal conflict. Furthermore, internet-addicts are likely to keep poor interpersonal relationships, which may induce more loneliness.

10.
Can J Infect Dis Med Microbiol ; 2019: 4851914, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31341522

RESUMO

Objectives: High dependency on pesticides could cause selection pressure leading to the development of resistance. This study was conducted to assess the resistance of the house fly, Musca domestica, to five insecticides, namely, permethrin, deltamethrin, beta-cypermethrin, propoxur, and dichlorvos, in Zhejiang Province. Methods: Field strains of house flies were collected from the 12 administrative districts in Zhejiang Province in 2011, 2014, and 2017, respectively. Topical application method was adopted for the bioassays. The probit analysis was used to determine the median lethal doses with the 95% confidence interval, and then the resistance ratio (RR) was calculated. The insecticides resistance in different years and the correlations of the resistance between different insecticides were also analyzed. Results: The resistance of field strains house flies to insecticides in Zhejiang Province was relatively common, especially for permethrin, deltamethrin, and beta-cypermethrin. The reversion of the resistance to dichlorvos was found, and most of the field strains in Zhejiang Province became sensitive to dichlorvos in 2017. Propoxur was much easier to cause very high level of resistance; the Hangzhou strain had the highest RR value more than 1000 in 2014, and five field strains had the RR value more than 100 in 2017. Compared to 2011 and 2014, the resistance of the house flies to propoxur and deltamethrin increased significantly in 2017. The resistance of permethrin, deltamethrin, beta-cypermethrin, and propoxur was significantly correlated with each other, and the resistance of dichlorvos was significantly correlated with beta-cypermethrin. Conclusions: Our results suggested that resistance was existed in permethrin, deltamethrin, beta-cypermethrin, and propoxur in the house flies of Zhejiang Province, while the resistance reversion to dichlorvos was found.

11.
Front Psychol ; 10: 1166, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31178792

RESUMO

This study aims to investigate sex differences in ratings for facial attractiveness (FA) and vocal attractiveness (VA). Participants (60 undergraduates in Study 1 and 111 undergraduates in Study 2) rated the attractiveness of computerized face images and voice recordings of men and women. In Study 1, face images and voice recordings were presented separately. Results indicated that men generally rated voice recordings of women more attractive than those of men, whereas women did not show different attractiveness ratings for voices of men vs. women. In Study 2, face images and voice recordings were paired as multimodal stimuli and presented simultaneously. Results indicated that men rated multimodal stimuli of women as more attractive than those of men, whereas women did not differentiate multimodal stimuli of men vs. women. We found that, compared to VA, FA had a stronger influence on participants' overall evaluations. Finally, we tested the difference between "original multimodal stimuli" (OMS) and "non-original multimodal stimuli" (non-OMS) and found the "OMS-facilitating effect." Taken together, findings indicated some sex differences in FA and VA in the current study, which could be used to interpret behaviors of sexual selection, human mate preferences, and designs and popularization of sex robots.

12.
Pathol Res Pract ; 215(7): 152427, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31072625

RESUMO

Aberrant expression of miR-145 was associated with chemotherapy in multitype cancers. However, the underlying role and molecular mechanism of miR-145 in the sensitivity of esophageal squamous cell carcinoma (ESCC) to 5-FU remained largely unknown. Cell viability was determined by Cell Counting Kit-8 (CCK-8) assay. Gene expression levels were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Protein expression levels were evaluated by Western blot. TargetScan was used for the prediction of binding sites for miRNA in mRNAs. The interaction between mRNA 3' UTR and miRNA was verified by dual luciferase reporter assay. The results showed that miR-145 was downregulated in ESCC tumor tissues and cells, while REV3L was upregulated in ESCC tumor tissues. Overexpression of miR-145 decreased REV3L mRNA and protein level in ESCC cell line KYSE150, while decreased miR-145 increased REV3L mRNA and protein level in esophageal epithelium cell line (HEEC). In addition, the luciferase activity of ESCC cells was decreased after the treatment of miR-145 mimic and mRNA 3'UTR-WT. Overexpressed miR-145 significantly inhibited cell viability and elevated cell apoptosis rate upon 5-FU treatment. Additionally, transfection of miR-145 mimic further altered expression of key genes involved in cell apoptosis (Bcl-2, Bax, Caspase3) in ESCC cells treated with 5-FU. miR-145 might be a therapeutic target for the treatment of ESCC.


Assuntos
Antineoplásicos/farmacologia , Proteínas de Ligação a DNA/metabolismo , DNA Polimerase Dirigida por DNA/metabolismo , Neoplasias Esofágicas/metabolismo , Carcinoma de Células Escamosas do Esôfago/metabolismo , Esôfago/patologia , Fluoruracila/farmacologia , MicroRNAs/metabolismo , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Neoplasias Esofágicas/genética , Neoplasias Esofágicas/patologia , Carcinoma de Células Escamosas do Esôfago/genética , Carcinoma de Células Escamosas do Esôfago/patologia , Esôfago/efeitos dos fármacos , Esôfago/metabolismo , Regulação Neoplásica da Expressão Gênica , Humanos , MicroRNAs/genética , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/fisiologia
13.
ACS Appl Mater Interfaces ; 11(20): 18511-18516, 2019 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-31059223

RESUMO

Multilayer InSe has emerged as a promising candidate for applications in novel electronic and optoelectronic devices due to its direct bandgap, high electron mobility, and excellent photoresponse with a broad response range. Here, we report synthesis of superlattice InSe nanosheets by simple thermal annealing for the first time. The mobility is increased to 299.1 cm2 V-1 s-1 for superlattice InSe FETs and is 4 times higher than 63.5 cm2 V-1 s-1 of pristine InSe device. The superlattice InSe photodetector shows an ultrahigh responsivity of 1.7 × 104 A/W (700 nm), which is 8.5 times greater than the pristine photodetector. Superlattice InSe photodetectors hold a good photoresponse stability and rapid response time of 20 ms. The electronic and photoresponse performance improvement of superlattice InSe is attributed to higher carrier sheet density and lower contact resistance for more effective electron injection and more photogenerated carrier injection, respectively. Those results suggest that superlattice is an effective method to further improve electronic and optoelectronic properties of two-dimensional InSe devices.

14.
Front Hum Neurosci ; 13: 132, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31114490

RESUMO

Facial attractiveness refers to a positive and joyful emotional experience induced by the face of a target person and the extent to which other people are driven to be close to their wishes. Since the 1970s, face attractiveness has gradually emerged in western psychological research, but most of the studies were confined to heterosexuals. More recently, some scholars have pointed out that sexual orientation may affect the judgment of facial attractiveness of individuals. Based on previous literature, this study proposed to explore the different facial attractiveness of individuals with different sexual orientations and sexual roles. Participants in this study were divided into two types (according to sexual orientation and sexual role) by the Sex Role Inventory for College Students (CSRI). Also, the eye-tracking technique was used to record the path of eye movements, where face images were manipulated by sexual dimorphism clues. The results showed that (1) compared to heterosexual men, homosexual men were significantly more likely to choose masculine faces as more attractive faces in paired faces; (2) male homosexuals are likely to have the feminization bias, and female homosexuals are likely to have the masculinization bias; and (3) the masculine faces are more attractive than feminine faces to participants whose sex role is feminine type and androgynous type.

15.
Plant Physiol Biochem ; 139: 411-418, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30981157

RESUMO

Potato tuber dormancy is critical for the postharvest quality. The supply of carbohydrates is considered as one of the important factors controlling the rate of potato tuber sprouting. Starch is the major carbohydrate reserve in potato tuber, but very little is known about the specific starch degrading enzymes responsible for controlling tuber dormancy and sprouting. In this study, we demonstrate that an α-amylase gene StAmy23 is involved in starch breakdown and regulation of tuber dormancy. Silencing of StAmy23 delayed tuber sprouting by one to two weeks compared with the control. This phenotype is accompanied by reduced levels of reducing sugars and elevated levels of malto-oligosaccharides in tuber cortex and pith tissue below the bud eye of StAmy23-deficient potato tubers. Changes in soluble sugars is accompanied by a slight variation of phytoglycogen structure and starch granule size. Our results suggest that StAmy23 may stimulate sprouting by hydrolyzing soluble phytoglycogen to ensure supply of sugars during tuber dormancy.


Assuntos
Germinação/fisiologia , Proteínas de Plantas/metabolismo , Tubérculos/metabolismo , Tubérculos/fisiologia , Solanum tuberosum/metabolismo , Solanum tuberosum/fisiologia , alfa-Amilases/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Germinação/genética , Proteínas de Plantas/genética , Tubérculos/genética , Solanum tuberosum/genética , Amido/metabolismo , Sacarose/metabolismo , Açúcares/metabolismo , alfa-Amilases/genética
16.
Int J Mol Sci ; 20(6)2019 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-30934614

RESUMO

Carbonic anhydrases (CAs) represent a group of enzymes that catalyse important reactions of carbon dioxide hydration and dehydration, a reaction crucial to many biological processes and environmental biotechnology. In this study we successfully constructed a thermostable fusion enzyme composed of the Sulfurihydrogenibium azorense carbonic anhydrase (Saz_CA), the fastest CA discovered to date, and the chitin binding domain (ChBD) of chitinase from Bacillus circulans. Introduction of ChBD to the Saz_CA had no major impact on the effect of ions or inhibitors on the enzymatic activity. The fusion protein exhibited no negative effects up to 60 °C, whilst the fusion partner appears to protect the enzyme from negative effects of magnesium. The prepared biocatalyst appears to be thermally activated at 60 °C and could be partially purified with heat treatment. Immobilisation attempts on different kinds of chitin-based support results have shown that the fusion enzyme preferentially binds to a cheap, untreated chitin with a large crystallinity index over more processed forms of chitin. It suggests significant potential economic benefits for large-scale deployment of immobilised CA technologies such as CO2 utilisation or mineralisation.


Assuntos
Bactérias/enzimologia , Dióxido de Carbono/metabolismo , Anidrases Carbônicas/metabolismo , Quitina/metabolismo , Proteínas Imobilizadas/metabolismo , Proteínas Recombinantes de Fusão/química , Temperatura , Água/metabolismo , Inibidores da Anidrase Carbônica/farmacologia , Anidrases Carbônicas/isolamento & purificação , Cristalização , Estabilidade Enzimática , Íons , Peso Molecular , Domínios Proteicos , Proteínas Recombinantes de Fusão/metabolismo
17.
Int J Mol Sci ; 20(2)2019 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-30646545

RESUMO

Abscisic acid (ABA) plays crucial roles in plant development and adaption to environmental stresses. The ABA-responsive element binding protein/ABRE-binding factor and ABA INSENSITIVE 5 (AREB/ABF/ABI5) gene subfamily members, which belong to the basic domain/leucine zipper (bZIP) transcription factors family, participate in the ABA-mediated signaling pathway by regulating the expression of their target genes. However, information about potato (Solanum tuberosum) AREB/ABF/ABI5 subfamily members remains scarce. Here, seven putative AREB/ABF/ABI5 members were identified in the potato genome. Sequences alignment revealed that these members shared high protein sequence similarity, especially in the bZIP region, indicating that they might possess overlapping roles in regulating gene expression. Subcellular localization analysis illustrated that all seven AREB/ABF/ABI5 members were localized in the nucleus. Transactivation activity assays in yeast demonstrated that these AREB/ABF/ABI5 members possessed distinct transcriptional activity. Electrophoretic mobility shift assays (EMSA) confirmed that all of these AREB/ABF/ABI5 members could have an affinity to ABRE in vitro. The expression patterns of these AREB/ABF/ABI5 genes showed that they were in response to ABA or osmotic stresses in varying degrees. Moreover, most AREB/ABF/ABI5 genes were induced during stolon swelling. Overall, these results provide the first comprehensive identification of the potato AREB/ABF/ABI5 subfamily and would facilitate further functional characterization of these subfamily members in future work.


Assuntos
Ácido Abscísico/genética , Genoma de Planta/genética , Desenvolvimento Vegetal/genética , Solanum tuberosum/genética , Proteínas de Arabidopsis/genética , Fatores de Transcrição de Zíper de Leucina Básica/genética , Regulação da Expressão Gênica de Plantas , Zíper de Leucina/genética , Pressão Osmótica , Plantas Geneticamente Modificadas , Ligação Proteica/genética , Estresse Fisiológico/genética
18.
Plant Physiol Biochem ; 136: 215-221, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30690278

RESUMO

Potato cold-induced sweetening (CIS) is a major drawback restricting potato process industry. Starch degradation and sucrose decomposition are considered to be the key pathways in potato CIS. Our previous study showed that the RING finger gene SbRFP1 could slow down starch degradation and the accumulation of reducing sugars (RS) through inhibiting amylase and invertase activity in cold-stored tubers. However, the regulation mechanism of SbRFP1 is not clear. In this paper, we first proved that SbRFP1 could promote starch synthesis and modify the shape of starch granules. By further yeast two hybrid, GST-pull down and inhibition of enzyme activity assays, we confirmed that SbRFP1 could slow down the transformation of starch to RS in tubers mainly through the inhibition of ß-amylase StBAM1 activity. SbRFP1 was also proved to possess E3 ubiquitin ligase activity by ubiquitination assay. Thus, SbRFP1 may regulate the accumulation of RS in cold-stored tubers by ubiquitination and degradation of StBAM1. Therefore, our study reveals the regulatory mechanism of SbRFP1 in the process of CIS and provides more powerful evidence for the effect of starch degradation on potato CIS.


Assuntos
Proteínas de Plantas/fisiologia , Tubérculos/crescimento & desenvolvimento , Domínios RING Finger/fisiologia , Solanum tuberosum/crescimento & desenvolvimento , Amilases/metabolismo , Temperatura Baixa , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Genes de Plantas/genética , Genes de Plantas/fisiologia , Proteínas de Plantas/genética , Domínios RING Finger/genética , Amido/metabolismo , Técnicas do Sistema de Duplo-Híbrido , Ubiquitina-Proteína Ligases/metabolismo
19.
Talanta ; 196: 442-448, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30683390

RESUMO

Fluorescent carbon quantum dots are emerging as a new class of fluorescent probes due to their unique optical properties. Herein, we presented a simple, one-pot and effective preparation strategy for copper-functionalized carbon quantum dots (Cu-CDs). The Cu-CDs demonstrated excitation-independent emissions, high fluorescent quantum yield (~ 24.4%) and excellent fluorescent stability. The fluorescence of Cu-CDs could be quenched efficiently in the presence of histidine (His) due to the coordination occurring between surface Cu and imidazole side chains of His. Thus, the Cu-CDs could be used as an efficient fluorescent probe for His detection. Under optimal conditions, the fluorescent signals of Cu-CDs decreased linearly with the concentrations of His over a range of 0.1-15 µM. The detection limit was 30 nM. The proposed method provided obvious advantages of rapid response, convenience, simplicity, high selectivity and sensitivity. Moreover, good results also have been obtained for His detection in real biological fluid samples.


Assuntos
Carbono/química , Cobre/química , Corantes Fluorescentes/química , Histidina/análise , Pontos Quânticos/química , Adulto , Histidina/química , Humanos , Micro-Ondas
20.
Mol Ther ; 27(2): 365-379, 2019 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-30341010

RESUMO

Laryngeal squamous cell carcinoma (LSCC) is a common form of head and neck cancer with poor prognosis. However, the mechanism underlying the pathogenesis of LSCC remains unclear. Here, we demonstrated increased expression of fascin actin-bundling protein 1 (FSCN1) and decreased expression of microRNA-145-5p (miR-145-5p) in a clinical cohort of LSCC. Luciferase assay revealed that miR-145-5p is a negative regulator of FSCN1. Importantly, low miR-145-5p expression was correlated with TNM (tumor, node, metastasis) status and metastasis. Moreover, cases with low miR-145-5p/high FSCN1 expression showed poor prognosis, and these characteristics together served as independent prognostic indicators of survival. Gain- and loss-of-function studies showed that miR-145-5p overexpression or FSCN1 knockdown inhibited LSCC migration, invasion, and growth by suppressing the epithelial-mesenchymal transition along with inducing cell-cycle arrest and apoptosis. Additionally, hypermethylation of the miR-145-5p promoter suggested that repression of miR-145-5p arises through epigenetic inactivation. LSCC tumor growth in vivo could be inhibited by using miR-145-5p agomir or FSCN1 small interfering RNA (siRNA), which highlights the potential for clinical translation. Collectively, our findings indicate that miR-145-5p plays critical roles in inhibiting the progression of LSCC by suppressing FSCN1. Both miR-145-5p and FSCN1 are important potential prognostic markers and therapeutic targets for LSCC.


Assuntos
Proteínas de Transporte/metabolismo , Metilação de DNA/fisiologia , Neoplasias de Cabeça e Pescoço/genética , Neoplasias de Cabeça e Pescoço/metabolismo , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/metabolismo , MicroRNAs/genética , Proteínas dos Microfilamentos/metabolismo , Regiões Promotoras Genéticas/genética , Animais , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/metabolismo , Proteínas de Transporte/genética , Linhagem Celular , Linhagem Celular Tumoral , Metilação de DNA/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Regulação Neoplásica da Expressão Gênica/fisiologia , Células HEK293 , Humanos , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , MicroRNAs/fisiologia , Proteínas dos Microfilamentos/genética
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