Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 405
Filtrar
1.
Small ; : e2103195, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34528386

RESUMO

Aqueous Zn-ion batteries own great potential on next generation wearable batteries due to the high safety and low cost. However, the uncontrollable dendrites growth and the negligible subzero temperature performance impede the batteries practical applications. Herein, it is demonstrated that dimethyl sulfoxide (DMSO) is an effective additive in ZnSO4 electrolyte for side reactions and dendrites suppression by regulating the Zn-ion solvation structure and inducing the Zn2+ to form the more electrochemical stable (002) basal plane, via the higher absorption energy of DMSO with Zn2+ and (002) plane. Moreover, the stable reconstructed hydrogen bonds between DMSO and H2 O dramatically lower the freezing point of the electrolyte, which significantly increases the ionic conductivity and cycling performance of the aqueous batteries at subzero temperatures. As a consequence, the symmetrical Zn/Zn cell can be kept stable for more than 2100 h at 20 °C and 1200 h at -20 °C without dendrite and by-products formation. The Zn/MnO2 batteries can perform steadily for more than 3000 cycles at 20 °C and 300 cycles at -20 °C. This work provides a facile and feasible strategy on designing high performance and dendrite free aqueous Zn-ion batteries for various temperatures.

2.
ACS Nano ; 2021 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-34546035

RESUMO

Semiconductor copper indium sulfide quantum dots are emerging as promising alternatives to cadmium- and lead-based chalcogenides in solar cells, luminescent solar concentrators, and deep-tissue bioimaging due to their inherently lower toxicity and outstanding photoluminescence properties. However, the nature of their emission pathways remains a subject of debate. Using low-temperature single quantum dot spectroscopy on core-shell copper indium sulfide nanocrystals, we observe two subpopulations of particles with distinct spectral features. The first class shows sharp resolution-limited emission lines that are attributed to zero-phonon recombination lines of a long-lived band-edge exciton. Such emission results from the perfect passivation of the copper indium sulfide core by the zinc sulfide shell and points to an inversion in the band-edge hole levels. The second class exhibits ultrabroad spectra regardless of the temperature, which is a signature of the extrinsic self-trapping of the hole assisted by defects in imperfectly passivated quantum dots.

3.
J Speech Lang Hear Res ; 64(9): 3685-3696, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34407380

RESUMO

Purpose During insertion of the cochlear implant electrode array, the tip of the array may fold back on itself and can cause serious complications to patients. This article presents a sensing system for cochlear implantation in a cochlear model. The electrode array fold-over behaviors can be detected by analyzing capacitive information from the array tip. Method Depending on the angle of the array tip against the cochlear inner wall when it enters the cochlear model, different insertion patterns of the electrode array could occur, including smooth insertion, buckling, and fold-over. The insertion force simulating the haptic feedback for surgeons and bipolar capacitance signals during the insertion progress were collected and compared. The Pearson correlation coefficient (PCC) was applied to the collected capacitive signals to discriminate the fold-over pattern. Results Forty-six electrode array insertions were conducted and the deviation of the measured insertion force varies between a range of 20% and 30%. The capacitance values from electrode pair (1, 2) were recorded for analyzing. A threshold for the PCC is set to be 0.94 that can successfully discriminate the fold over insertions from the other two types of insertions, with a success rate of 97.83%. Conclusions Capacitive measurement is an effective method for the detection of faulty insertions and the maximization of the outcome of cochlear implantation. The proposed capacitive sensing system can be used in other tissue implants in vessels, spinal cord, or heart.

4.
Exp Anim ; 2021 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-34349080

RESUMO

Minocycline is a type of tetracycline antibiotic with broad-spectrum antibacterial activity that has been demonstrated to protect the brain against a series of central nervous system diseases. However, the precise mechanisms of these neuroprotective actions remain unknown. In the present study, we found that minocycline treatment significantly reduced HT22 cell apoptosis in a mechanical cell injury model. In addition, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining confirmed the neuroprotective effects of minocycline in vivo through the inhibition of apoptosis in a rat model of controlled cortical impact (CCI) brain injury. The western blotting analysis revealed that minocycline treatment significantly downregulated the pro-apoptotic proteins BAX and cleaved caspase-3 and upregulated the anti-apoptotic protein BCL-2. Furthermore, the beam-walking test showed that the administration of minocycline ameliorated traumatic brain injury (TBI)-induced deficits in motor function. Taken together, these findings suggested that minocycline attenuated neuronal apoptosis and improved motor function following TBI.

5.
Physiol Plant ; 2021 Aug 12.
Artigo em Inglês | MEDLINE | ID: mdl-34389991

RESUMO

Water deficit stress severely threatens crop yield and numerous reports have shown silicon could enhance plants resistance to water deficit. One of the most important mechanisms is that silicon maintains the water balance. In this review, we summarized advanced research to elucidate the effect of silicon on plant water transport processes, including leaf water loss, vessel water transport, and root water uptake. In leaves, the deposition of silica phytolith on cuticle and stomata decreases transpirational water loss under water deficit stress. However, accumulating evidence suggest that silicon maintaining leaf water content is not through reducing water loss, but through osmotic adjustments, enhancing water transport and uptake. Enhancement of stem water transport efficiency by silicon is due to silica phytolith depositing in the cell wall of vessel tubes and pits, which support it avoiding to collapse and embolism, respectively. The improvement of root water uptake capacity by silicon acts as a key role in maintaining water balance. The underlying mechanisms include (i) enlargement of the root water uptake area, (ii) improvement of the water driving force, (iii) the prevention of water loss from root to soil, and (iv) the up-regulation of aquaporin activity. This review provides three simple models to understand the mechanism of silicon on water balance and highlights the future research area.

6.
J Sci Food Agric ; 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34265089

RESUMO

BACKGROUND: The beneficial function of phytase and 25-hydroxyvitamin D3 (HyD) on the feed utilization rate has been widely investigated. However, studies concerning its influence on weaned piglets largely lag behind. The aim of this study was to investigate the effects of phytase and HyD supplementation on the growth performance and bone development in weaned piglets under dietary Ca and P deficiency. RESULTS: The results showed that dietary Ca and P deficiency decreased (P < 0.05) the content of serum P in 6-10 kg piglets, as well as reducing (P < 0.05) the contents of serum Ca and P, average daily gain (ADG), bone mineral density (BMD), breaking force (BF), bone ash and femur Ca in 10-20 kg piglets. Compared with the control group, the feed-to-gain ratio (F/G) of 6-10 kg piglets in the Phy group was decreased (P < 0.05), whereas the ADG, blood Ca and P, BMD, BF, bone ash, P apparent digestibility, Ca and P retention rate of 10-20 kg piglets were increased (P < 0.05). The contents of serum osteocalcin and HyD in 6-10 kg piglets and ADG were higher than in the control group (P < 0.05), as well as the contents of serum Ca and HyD in 10-20 kg piglets in the HyD treatment group. Supplementation with both Phy and HyD decreased the F/D (P < 0.05) and increased the contents of serum Ca, P and HyD in 6-10 kg piglets as well as enhancing the ADG, BMD, BF, bone ash, femur Ca and P, serum Ca and P, HyD, and the apparent digestibility and retention of Ca and P (P < 0.05) in 10-20 kg piglets. Supplementation with Phy and HyD in Ca- and P-deficient dietary decreased bone resorption, and improved tight arrangement of collagen fibers and oblique fibers in weaned piglets. CONCLUSION: These data indicated that supplementation with both 1500 U kg-1 Phy and 50 µg kg-1 HyD could enhance dietary Ca and P utilization and promote bone development in low Ca and P dietary, and supplementation with both Phy and HyD had a significant synergy effect compared to single supplement. © 2021 Society of Chemical Industry.

7.
Nat Genet ; 53(8): 1156-1165, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34211177

RESUMO

The most prevalent post-transcriptional mRNA modification, N6-methyladenosine (m6A), plays diverse RNA-regulatory roles, but its genetic control in human tissues remains uncharted. Here we report 129 transcriptome-wide m6A profiles, covering 91 individuals and 4 tissues (brain, lung, muscle and heart) from GTEx/eGTEx. We integrate these with interindividual genetic and expression variation, revealing 8,843 tissue-specific and 469 tissue-shared m6A quantitative trait loci (QTLs), which are modestly enriched in, but mostly orthogonal to, expression QTLs. We integrate m6A QTLs with disease genetics, identifying 184 GWAS-colocalized m6A QTL, including brain m6A QTLs underlying neuroticism, depression, schizophrenia and anxiety; lung m6A QTLs underlying expiratory flow and asthma; and muscle/heart m6A QTLs underlying coronary artery disease. Last, we predict novel m6A regulators that show preferential binding in m6A QTLs, protein interactions with known m6A regulators and expression correlation with the m6A levels of their targets. Our results provide important insights and resources for understanding both cis and trans regulation of epitranscriptomic modifications, their interindividual variation and their roles in human disease.


Assuntos
Adenosina/análogos & derivados , Encéfalo/fisiologia , Pulmão/fisiologia , Músculo Esquelético/fisiologia , Locos de Características Quantitativas , Adenosina/genética , Adenosina/metabolismo , Estudo de Associação Genômica Ampla , Coração/fisiologia , Humanos , Metilação , Especificidade de Órgãos , Polimorfismo de Nucleotídeo Único , Processamento Pós-Transcricional do RNA , Proteínas de Ligação a RNA/genética , Reprodutibilidade dos Testes
8.
J Am Heart Assoc ; 10(15): e020187, 2021 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-34315237

RESUMO

Background This Mendelian randomization study aims to investigate causal associations between genetically predicted insomnia and 14 cardiovascular diseases (CVDs) as well as the potential mediator role of 17 cardiometabolic risk factors. Methods and Results Using genetic association estimates from large genome-wide association studies and UK Biobank, we performed a 2-sample Mendelian randomization analysis to estimate the associations of insomnia with 14 CVD conditions in the primary analysis. Then mediation analysis was conducted to explore the potential mediator role of 17 cardiometabolic risk factors using a network Mendelian randomization design. After correcting for multiple testing, genetically predicted insomnia was consistent significantly positively associated with 9 of 14 CVDs, those odds ratios ranged from 1.13 (95% CI, 1.08-1.18) for atrial fibrillation to 1.24 (95% CI, 1.16-1.32) for heart failure. Moreover, genetically predicted insomnia was consistently associated with higher body mass index, triglycerides, and lower high-density lipoprotein cholesterol, each of which may act as a mediator in the causal pathway from insomnia to several CVD outcomes. Additionally, we found very little evidence to support a causal link between insomnia with abdominal aortic aneurysm, thoracic aortic aneurysm, total cholesterol, low-density lipoprotein cholesterol, glycemic traits, renal function, and heart rate increase during exercise. Finally, we found no evidence of causal associations of genetically predicted body mass index, high-density lipoprotein cholesterol, or triglycerides on insomnia. Conclusions This study provides evidence that insomnia is associated with 9 of 14 CVD outcomes, some of which may be partially mediated by 1 or more of higher body mass index, triglycerides, and lower high-density lipoprotein cholesterol.

9.
Huan Jing Ke Xue ; 42(7): 3166-3175, 2021 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-34212642

RESUMO

Microplastics have been found in many environmental media such as sea water, coastal tidal flats, terrestrial water, sediments, and organisms. Microplastics pollution in inland freshwater lakes have received extensive attention; however, the correlation between eutrophication and microplastics pollution in freshwater lakes remains unclear. In this study, 24 sampling sites were set up in the near shore surface waters of Dianchi Lake, and the pollution characteristics of microplastics such as abundance, composition, particle size, color, and form were evaluated. Water quality parameters related to eutrophication state were analyzed, and the eutrophication indices were further calculated. Specifically, sample pre-treatment was conducted according to the method issued by National Oceanic and Atmospheric Administration (NOAA) of the United States. The color and morphological characteristics of microplastic samples were observed using a stereoscopic microscope, and counts and particle size measurements were performed using Nano Measure 1.2 software. Parts of the samples were selected, and the polymer composition analysis was performed using micro-Fourier Transform infrared (µ-FTIR) spectroscopy. The indices related to eutrophication level evaluation were tested according to the experimental standard methods issued by the Ministry of Ecology and Environment of China. The results showed that the abundance of microplastics in the near shore waters of Dianchi Lake was between 800 and 6000 n·m-3, with an average value of 2867 n·m-3. The types of polymers detected were polyethylene terephthalate (PET), polyetherurethane (PEU), polypropylene (PP), polyethylene (PE), and polyvinyl acetate (PVAc), respectively. The diameter proportion of microplastics in the range of 0.2-0.5 mm was the highest. Fiber microplastics accounted for the most observed type, followed by fragments and films. Among the 24 monitoring sites, it was found that proportions of severe, moderate, and mild eutrophication and mesotrophication sites accounted for 8.33%, 58.33%, 29.17%, and 4.17% of the total sampling sites, respectively, and the main pollutant was total nitrogen (TN). Microplastics abundances in the near shore waters of Dianchi Lake were significantly positively correlated with TN concentrations (P<0.01), whereas they were negatively correlated with chlorophyll a(Chl-a)concentrations, not reaching a significant level (P>0.05). The microplastics abundance and TN concentrations in the north bank water near the main urban area of Kunming were significantly higher than those in the other three banks. Microplastics and TN were considered to potentially have the same origin and be attributed to the tail water discharge from WWTPs.


Assuntos
Microplásticos , Poluentes Químicos da Água , China , Clorofila A , Monitoramento Ambiental , Eutrofização , Sedimentos Geológicos , Lagos/análise , Plásticos , Poluentes Químicos da Água/análise
10.
Inorg Chem ; 2021 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-34232028

RESUMO

The new indium-based organic framework {(Me2NH2)[In(BDPO)]·DMF·2H2O}n (1) was successfully constructed by using the oxalamide group modified ligand N,N'-bis(isophthalic acid)oxalamide (H4BDPO). This framework presents a 2-fold interpenetrating structural characteristic, and the unique polar pore environment leads to a high capture ability for CO2, C2Hn and CH3OH and good separation ability for CO2 and C2Hn over CH4 as well as for CH3OH over C2H5OH, which was further verified by an ideal adsorbed solution theory (IAST) calculation. Theoretical simulations pointed out the possible adsorption sites of different adsorbed gases in 1. In addition, the excellent chemical stability and strong luminescence of 1 give it an effective selective detection ability for 2,6-dichloro-4-nitroaniline (DCN) in water with a low detection limit of 3.85 ppm, and the detection mechanism is discussed in detail.

11.
Aging (Albany NY) ; 13(12): 16024-16042, 2021 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-34133324

RESUMO

Prostate adenocarcinoma is one of the leading adult malignancies. Identification of multiple causative biomarkers is necessary and helpful for determining the occurrence and prognosis of prostate adenocarcinoma. We aimed to identify the potential prognostic genes in the prostate adenocarcinoma microenvironment and to estimate the causal effects simultaneously. We obtained the gene expression data of prostate adenocarcinoma from TCGA project and identified the differentially expressed genes based on immune-stromal components. Among these genes, 68 were associated with biochemical recurrence at 3 years after prostatectomy in prostate adenocarcinoma. After adjusting for the minimal sets of confounding covariates, 14 genes (TNFRSF4, ZAP70, ERMN, CXCL5, SPINK6, SLC6A18, CHRM2, TG, CLLU1OS, POSTN, CTSG, NETO1, CEACAM7, and IGLV3-22) related to the microenvironment were identified as prognostic biomarkers using the targeted maximum likelihood estimation. Both the average and individual causal effects were obtained to measure the magnitude of the effect. CIBERSORT and gene set enrichment analyses showed that these prognostic genes were mainly associated with immune responses. POSTN and NETO1 were correlated with androgen receptor expression, a main driver of prostate adenocarcinoma progression. Finally, five genes were validated in another prostate adenocarcinoma cohort (GEO: GSE70770). These findings might lead to the improved prognosis of prostate adenocarcinoma.


Assuntos
Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Biomarcadores Tumorais/metabolismo , Recidiva Local de Neoplasia/patologia , Prostatectomia , Neoplasias da Próstata/patologia , Neoplasias da Próstata/cirurgia , Microambiente Tumoral , Adenocarcinoma/genética , Adenocarcinoma/imunologia , Fatores de Confusão Epidemiológicos , Bases de Dados Genéticas , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Ontologia Genética , Genoma Humano , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Neoplasias da Próstata/genética , Neoplasias da Próstata/imunologia , Receptores Androgênicos/metabolismo , Reprodutibilidade dos Testes , Células Estromais/metabolismo , Microambiente Tumoral/genética
12.
Poult Sci ; 100(8): 101238, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34157559

RESUMO

Hypervirulent fowl adenovirus serotype 4 (hvFAdV-4) has emerged as a major pathogen of hepatitis-hydropericardium syndrome (HHS) with increased mortality in chickens, resulting in economic losses to the Chinese poultry industry since June 2015. Here, we isolated a hypervirulent FAdV-4 (hvFAdV-4) strain (designated GD616) from 25-day-old meat-type chickens with severe HHS in Guangdong Province China in June 2017. The whole genome of the strain GD616 shares high homology with those in the recently-reported hvFAdV-4 isolates in China, with natural deletions of ORF19 and ORF27. A comparative analysis of Hexon and Fiber-2 proteins revealed that 2 unique amino acid residues at positions 378 and 453 of the Fiber-2 protein might be associated with virulence due to their occurrences in all the hvFAdV-4 isolates only. To systemically evaluate the effect of age on the susceptibility of chickens to hvFAdV-4, we used this hvFAdV-4 strain to intramuscularly inoculate 7- to 180-day-old specific-pathogen-free chickens for the evaluation of pathogenicity. These results showed that the pathogenicity of the hvFAdV-4 strain GD616 to chickens exhibited age-relatedness, with younger than 59-day-old chickens showing 100% morbidity and mortality, while 180-day-old chickens still exhibited a hydropericardium syndrome-like clinicopathology with 60% morbidity and 20% mortality. These findings enrich the current available knowledge regarding the pathogenicity of the hypervirulent FAdV-4 virus in chickens with a wide range of ages, which assists with the selection of suitable-aged chickens for the evaluation of hvFAdV-4 vaccines.


Assuntos
Infecções por Adenoviridae , Aviadenovirus , Doenças das Aves Domésticas , Adenoviridae , Infecções por Adenoviridae/veterinária , Animais , Aviadenovirus/genética , Galinhas , China , Filogenia , Sorogrupo , Virulência
13.
Int J Cardiol ; 339: 179-184, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34181993

RESUMO

BACKGROUND: Although observational studies have shown an association between sex hormone-binding globulin (SHBG), testosterone (T) and cardiovascular diseases (CVD), controversy remains. In this study, we aim to explore the causal effects of SHBG and T on Coronary heart disease (CHD). METHODS: We used univariable, network and multivariable mendelian randomization (MR) analysis to investigate the causal effect of SHBG and T on CHD. We performed inverse variance weighted (IVW) MR as the primary analysis, with the robustness of this approach further tested by other methods in sensitivity analysis. The SHBG and T were collected from the UK Biobank data, about 180,000 men aged 40 to 69 years. CHD was collected from CARDIoGRAMplusC4D 1000 Genomes-based GWAS, which was a meta-analysis including 48 studies and involving 60,801 CHD cases and 123,504 controls. RESULTS: Using univariable MR-IVW, the results suggested that a one standard deviation (SD) increase in SHBG, the risk of CHD decreased by approximately 14% (OR (95% CI): 0.86(0.76,0.97)), and that a SD increase in total testosterone (TT), the risk also decreased, approximately 8% (OR (95% CI): 0.92(0.85,0.99)). Multivariable MR showed that both SHBG and TT had no direct causal effect with CHD (a SD increase in SHBG: OR (95% CI):0.75(0.57,1.00), P = 0.053; a SD increase in TT: OR (95% CI): 1.05(0.90,1.22), P = 0.53). In the network MR analysis, the results suggested that TT might act as mediator in the causal pathway from SHBG to CHD and account for 93% of the total effect of SHBG on CHD, and that SHBG might be a mediator in the causal pathway from TT to CHD and account for 67% of the total effect of TT on CHD. CONCLUSIONS: Genetically predicted SHBG and TT were negatively correlated with CHD in both univariable and network MR, which may provide a causal explanation behind the observed conclusion. In addition, TT and SHBG had a bidirectional causal effect. Further work is required to disentangle the downstream effects of SHBG/TT on CHD and the molecular pathways involved, as the simultaneous regulation of SHBG and TT may make it a viable strategy for the prevention or treatment of CHD.

14.
Artigo em Inglês | MEDLINE | ID: mdl-34106488

RESUMO

Data from 655 treatments of 116 studies were used in a meta-analysis to determine the daily digestible energy (DE), metabolizable energy (ME) and net energy (NE) intake of Chinese growing-finishing pigs, and to predict feed efficiency responses to change in dietary DE, ME and NE. Three alternative functions (i.e., polynomial, Bridges and asymptotic function) were employed for fitting daily DE, ME or NE intakes to mean body weight. The results showed that the three models from the current study provided reasonable fit (all R2  > 0.83) for the energy intake data. However, under the same energy system, the polynomial function had the smallest Akaike's information criteria (AIC) and residual standard deviation (RSD), followed by Bridges and asymptotic functions. The three model-generated energy intakes of growing pigs were significantly less than that of the Chinese Feeding Standard of Swine, but similar to that of the National Research Council (2012), while the values of finishing pigs were greater than both standards. Compared with those that predict feed efficiency based on DE or ME, the equation with NE as a predictor had the minimized AIC and RSD. It was also found that feed efficiency increased with increasing dietary energy density (DED), but this response varied with pig body weight, and the lighter pigs were more sensitive to DED than heavier pigs.

16.
Colloids Surf B Biointerfaces ; 205: 111903, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34144323

RESUMO

A combination of photodynamic therapy (PDT) and histone deacetylase inhibitor (HDACis) could potentiate single-mode anti-tumor activity of HDACis or PDT to inhibit tumor relapse and metastasis. However, poor solubility and heterogeneity in cellular uptake and tissue distribution hamper the dual mode antitumor effect. For a controlled drug release of photosensitizers and HDACis in cytoplasm, photosensitizer pyropheophorbide-a (Pyro) encapsulated in polymer polyethylene glycol-b-poly (asparaginyl-vorinostat) (simplified as Pyro@FPPS) are fabricated to achieve their lysosomal spatiotemporal synchronized release. With HDACis modeling PDT in vitro and in vivo, it seems that polymerized Vorinostat encapsulated photosensitizers significantly inhibited the tumor proliferation and metastasis by spatiotemporal synchronized drugs release, and Pyro@FPPS reported here reveals a promising prospect to exert drugs' synergistic effect in a spatiotemporal synchronized manner and can be an effective strategy to inhibit tumor growth, recurrence and metastasis in clinic.


Assuntos
Antineoplásicos , Fotoquimioterapia , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Liberação Controlada de Fármacos , Inibidores de Histona Desacetilases/farmacologia , Lisossomos , Fármacos Fotossensibilizantes/farmacologia , Vorinostat/farmacologia
17.
Dalton Trans ; 50(17): 5713-5723, 2021 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-33949548

RESUMO

Indium metal-organic frameworks (In-MOFs) based on pyridylcarboxylate ligands represent a subclass of MOFs featuring diverse structures, a high stability, and various properties. This review discusses the different aspects of In-MOFs including their design, synthesis and structures as well as their typical potential applications in adsorption and separation, catalysis, and chemical sensors. Importantly, the effect of pyridine on the properties and stability of frameworks has been carefully studied. The introduction of a pyridine group not only significantly enriches clusters of In3+ ions, but also enables flexible, controllably synthesized ionic or neutral frameworks to be fabricated. Based on this, we suggest that this type of In-metal organic framework (MOF) should receive more attention in the field of MOF design.

18.
Dalton Trans ; 2021 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-34002183

RESUMO

The isolable anionic oxoborane 3 and thioxoborane 4 have been assembled using a 1,2-bis(imino)acenaphthene ligand (Dip-BIAN). Structural characterization and DFT calculations confirmed that two compounds contain terminal doubly bonded B[double bond, length as m-dash]E (E = O, S) groups, respectively, in which only the B[double bond, length as m-dash]O group is associated with imidazolium via a hydrogen bond.

19.
Drug Deliv ; 28(1): 963-972, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34036867

RESUMO

PD-1/PD-L1 blocking therapy has become one of the most promising methods in the field of tumor treatment. However, it encounters the challenge of immune escape due to the exhaustion of T cells. Studies have shown that the epigenetic regulation drug histone deacetylase inhibitor (HDACi) may be able to reverse exhausted T cells by changing the epigenetic transcription program. Therefore, the combination of epigenetic therapy and PD-1/PD-L1 blockade therapy is expected to reverse the immune escape, whereas the overriding goal should aim at the spontaneous release and synergy of PD-1/PD-L1 blocking siRNA and HDACi. In this study, we develop PDDS{polyethylene glycol-b-asparaginate(diethylenetriamine-vorinostat), (PEG-b-P[Asp(DET-SAHA)n] PPDS)}encapsulating siRNA-PD-L1to provide micelles siRNA-PD-L1-loaded micelles (siRNA@PPDS). Transmission electron microscope (TEM) images demonstrate that siRNA@PPDS micelles presented spherical morphology with a size of about 120 nm; hydrodynamic data analysis indicates pH sensitivity of siRNA@PPDS micelles. The experiments reveal that siRNA@PPDS micelles could be well uptaken by the tumor cells to silence the expression of PD-L1 protein in a dose-dependent manner; compared with the free SAHA, the SAHA-loaded micelles PPDS show higher cytotoxicity to induce tumor cell apoptosis and block cell cycle in G1 phase on melanoma-bearing mice, siRNA@PPDS has shown outstanding inhibition of tumor growth and pulmonary metastasis. By comprehensively activating the immune system, lysosome activable polymeric vorinostat encapsulating PD-L1KD for the combination therapy of PD-L1-KD and HDACIs can be an effective strategy to reverse the unresponsiveness of immune checkpoint inhibitors and a promising treatment to inhibit tumor growth, recurrence, and metastasis in clinic.

20.
Gene ; 791: 145722, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34010708

RESUMO

Plant height is a fundamentally crucial agronomic trait to control crop growth and high yield cultivation. Several studies have been conducted on the understanding ofmolecular genetic bases of plant height in model plants and crops. However, the molecular mechanism underlying peanut plant height development is stilluncertain. In the present study, we created a peanut mutant library by fast neutron irradiation using peanut variety SH13 and identified a semi-dwarf mutant 1 (sdm1). At 84 DAP (days after planting), the main stem of sdm1 was only about 62% of SH13. The internode length of sdm1 hydroponic seedlings was found significantly shorter than that of SH13 at 14 DAP. In addition, the foliar spraying of exogenous IAA could partially restore the semi-dwarf phenotype of sdm1. Transcriptome data indicated that the differentially expressed genes (DEGs) between sdm1 and SH13 significantly enriched in diterpenoid biosynthesis, alpha-linolenic acid metabolism, brassinosteroid biosynthesis, tryptophan metabolism and plant hormone signal transduction. The expression trend of most of the genes involved in IAA and JA pathway showed significantly down- and up- regulation, which may be one of the key factors of the sdm1 semi-dwarf phenotype. Moreover, several transcription factorsand cell wall relatedgenes were expressed differentially between sdm1 and SH13. Conclusively, this research work not only provided important clues to unveil the molecular mechanism of peanut plant height regulation, but also presented basic materials for breeding peanut cultivars with ideal plant height.


Assuntos
Arachis/crescimento & desenvolvimento , Arachis/genética , Regulação da Expressão Gênica de Plantas/genética , Biometria/métodos , Fabaceae/genética , Fabaceae/crescimento & desenvolvimento , Expressão Gênica/genética , Perfilação da Expressão Gênica/métodos , Genômica/métodos , Fenótipo , Melhoramento Vegetal/métodos , Reguladores de Crescimento de Plantas/metabolismo , RNA-Seq/métodos , Plântula/genética , Transcriptoma/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...