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1.
Zhonghua Yi Xue Za Zhi ; 100(4): 279-285, 2020 Feb 04.
Artigo em Chinês | MEDLINE | ID: mdl-32075356

RESUMO

Objective: To study the technical notes and clinical efficacy of full-endoscopic thoracic intervertebral discectomy via transforaminal approach. Methods: We included 16 patients with thoracic disc herniation treated by full-endoscopic thoracic discectomy via transforaminal approach between January 2017 and September 2018 in ours department of orthopedics. The average age is 53.7 years. The compressionare classified by nature: 5 cases of soft thoracic disc herniation, 7 cases of calcified or ossified thoracic disc herniation, and 4 cases of osteophyte protrusion of the posterior edge of the adjacent vertebral body of the diseased disc. All patients had symptoms of thoracic myelopathy before operation, and 7 of them had radiculopathy. Via transforaminal approach under local anesthesia, enlarged foraminoplasty and full-endoscopic thoracic discectomy were used for treatment. Observe the changes of postoperative imaging, pain symptoms and recovery of spinal cord function at 1 week, 3 months, 6 months and 1 year after operation. Back pain and radicular pain were scored with VAS, neurological function was assessed with Nurick score and mJOA score, and thoracic spine function was assessed with Oswestry disability index (ODI). Results: All operations were successfully completed, and no intraoperative conversion of surgical methods occurred. Postoperative thoracic MRI and CT examinations of all patients showed that the spinal cord was fully decompressed without any residual compression. Back pain and radicular pain were all relieved obviously in all patients, and spinal cord function was obviously restored. Transient intercostal neuralgia occurred in 2 cases after operation, and no other surgical complications occurred. Conclusions: Full-endoscopic or fluoroscopic foraminoplasty and full-endoscopic thoracic discectomyvia transforaminal approach under local anesthesia is a safe and effective treatment for soft and hard thoracic disc herniation located on the ventral side of the spinal cord.


Assuntos
Discotomia Percutânea , Deslocamento do Disco Intervertebral , Endoscopia , Humanos , Vértebras Lombares , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
3.
Curr Top Dev Biol ; 121: 311-337, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28057305

RESUMO

Recent studies suggest that a small subset of cells within a tumor, the so-called cancer stem cells (CSCs), are responsible for tumor propagation, relapse, and the eventual death of most cancer patients. CSCs may derive from a few tumor-initiating cells, which are either transformed normal stem cells or reprogrammed differentiated cells after acquiring initial cancer-causing mutations. CSCs and normal stem cells share some properties, but CSCs differ from normal stem cells in their tumorigenic ability. Notably, CSCs are usually resistant to chemo- and radiation therapies. Despite the apparent roles of CSCs in human cancers, the biology underlying their behaviors remains poorly understood. Over the past few years, studies in Drosophila have significantly contributed to this new frontier of cancer research. Here, we first review how stem-cell tumors are initiated and propagated in Drosophila, through niche appropriation in the posterior midgut and through stem-cell competition for niche occupancy in the testis. We then discuss the differences between normal and tumorigenic stem cells, revealed by studying RasV12-transformed stem-cell tumors in the Drosophila kidney. Finally, we review the biology behind therapy resistance, which has been elucidated through studies of stem-cell resistance and sensitivity to death inducers using female germline stem cells and intestinal stem cells of the posterior midgut. We expect that screens using adult Drosophila neoplastic stem-cell tumor models will be valuable for identifying novel and effective compounds for treating human cancers.


Assuntos
Envelhecimento/fisiologia , Carcinogênese/patologia , Drosophila/fisiologia , Células-Tronco Neoplásicas/patologia , Animais , Humanos , Modelos Biológicos , Especificidade de Órgãos
4.
Neuroscience ; 286: 27-36, 2015 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-25463519

RESUMO

Spinal cord injury (SCI) represents a severe health problem worldwide usually associated with severe disability and reduced quality of life. The aim of this work was to investigate the role of prohibitin 1 (PHB1) in the progression of SCI in rats. Firstly, we observed that expression of PHB1 was downregulated following SCI in rats. Then, we hypothesized that PHB1 overexpression by delivery of Ad-PHB1 could result in neuroprotection and promote functional recovery following SCI. Briefly, Wistar rats received a 35-g clip-compression injury and were administered Ad-PHB1 or Ad immediately following SCI. It was found that Ad-PHB1 administration significantly improved locomotor function and increased pain tolerance in rats with SCI. Furthermore, Ad-PHB1 administration following SCI attenuated axonal degradation and increased neuron sparing. Ad-PHB1 administration following SCI reduced apoptosis through inhibiting the Bcl-2/Bax/caspase-3 pathway. Ad-PHB1 administration following SCI suppressed endoplasmic reticulum stress, evidenced by reduced mRNA levels of CCAAT enhancer binding protein homologous protein, chaperone-ucose-regulated protein 78, and X-box protein 1. Ad-PHB1 administration following SCI restored mitochondrial adenosine triphosphate formation, reduced reactive oxygen species formation, and improved mitochondrial respiration rates. Finally, Ad-PHB1 administration following SCI activated downstream signals including phosphatidylinositol-3-kinase (PI3K)/Akt, extracellular signal-regulated kinase (ERK1/2), and nuclear factor-kappaB. These data indicate that the PHB1 plays an important role in the development of SCI and might provide a therapeutic target to promote recovery from SCI.


Assuntos
Fármacos Neuroprotetores/metabolismo , Fármacos Neuroprotetores/uso terapêutico , Recuperação de Função Fisiológica/efeitos dos fármacos , Proteínas Repressoras/metabolismo , Proteínas Repressoras/uso terapêutico , Traumatismos da Medula Espinal/tratamento farmacológico , Traumatismos da Medula Espinal/metabolismo , Adenoviridae , Animais , Apoptose/efeitos dos fármacos , Progressão da Doença , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Feminino , Vetores Genéticos , Hiperalgesia/tratamento farmacológico , Locomoção/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Ratos , Ratos Wistar , Proteínas Repressoras/genética , Transdução de Sinais/efeitos dos fármacos , Traumatismos da Medula Espinal/genética
5.
Eur Rev Med Pharmacol Sci ; 18(5): 740-52, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24668718

RESUMO

BACKGROUND: The effect of injectable demineralized bone matrix (DBM) on bone repair is not known. Here, we tested the hypothesis that injectable DBM can heal a critical-size diaphyseal radius defect in a rabbit model. MATERIALS AND METHODS: The bone defect was filled with DBM powder, injectable DBM or powdered, freeze-dried powdered allografts. Radiological determination, gross evaluation, histology, and micro-computer tomography was carried out 4, 8, and 12 weeks after the surgery, respectively. RESULTS: The injectable DBM group yielded better when compared with the freeze-dried powder group (p < 0.05). Moreover, biomechanical functionality was restored comparable to normal levels in the injectable DBM group. CONCLUSIONS: The injectable DBM was as effective in structurally and functionally repairing bone defects as the DBM powder and more effective than the freeze-dried bone powder. Thus, our study supports the use of injectable DBM for bone healing.


Assuntos
Técnica de Desmineralização Óssea/métodos , Matriz Óssea/transplante , Quitosana/administração & dosagem , Modelos Animais de Doenças , Rádio (Anatomia)/lesões , Cicatrização/efeitos dos fármacos , Animais , Materiais Biocompatíveis/administração & dosagem , Liofilização , Masculino , Coelhos , Radiografia , Rádio (Anatomia)/diagnóstico por imagem , Transplante Homólogo/métodos , Cicatrização/fisiologia
6.
Oncogene ; 25(44): 5933-41, 2006 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-16636660

RESUMO

Birt-Hogg-Dubé syndrome (BHD) is a rare, inherited genodermatosis characterized by hair follicle hamartomas, kidney tumors and spontaneous pneumothorax. The BHD locus was mapped to chromosome 17p11.2 by linkage analysis, and germline mutations in a novel gene (BHD) were identified in a panel of BHD families. Using RNA interference to decrease expression of the Drosophila BHD homolog (DBHD), we have demonstrated that DBHD is required for male germline stem cell (GSC) maintenance in the fly testis. Reduction of DBHD gene activity suppresses the GSC overproliferation phenotype associated with overexpression of either unpaired (upd) or decapentaplegic (dpp). Further genetic interaction experiments suggest that DBHD regulates GSC maintenance downstream or in parallel of the JAK/STAT and Dpp signal-transduction pathways. These findings suggest that the BHD protein may regulate tumorigenesis through modulating stem cells in human.


Assuntos
Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/fisiologia , Drosophila/genética , Proteínas Tirosina Quinases/metabolismo , Proteínas Proto-Oncogênicas/genética , Fatores de Transcrição STAT/metabolismo , Transdução de Sinais/genética , Espermatozoides/citologia , Células-Tronco/metabolismo , Testículo/metabolismo , Fatores de Transcrição/metabolismo , Sequência de Aminoácidos , Animais , Diferenciação Celular/genética , Humanos , Janus Quinases , Masculino , Dados de Sequência Molecular , Proteínas/fisiologia , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas/fisiologia , Interferência de RNA , Espermatozoides/metabolismo , Células-Tronco/citologia , Testículo/citologia , Proteínas Supressoras de Tumor/genética , Proteínas Supressoras de Tumor/metabolismo , Proteínas Supressoras de Tumor/fisiologia
7.
J Chromatogr B Analyt Technol Biomed Life Sci ; 831(1-2): 163-8, 2006 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-16406380

RESUMO

Sodium tanshinone IIA sulfonate (STS), a hydrophilic ionic substance, is used as a cardiovascular drug. An ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC) method for the determination of STS in mouse plasma was initially developed. The assay involved a rapid and simple extraction process and subsequent detection at 271 nm. The retention time for STS was 7.5 min. Based on extracted STS standard mouse plasma at 1.5,10 and 50 microg/ml, the assay precision were 2.7, 2.1 and 1.7% with a mean accuracy of 96.7, 98.5 and 99.4%, respectively. At plasma concentration of 1.5, 50 and 75 microg/ml, the mean recovery of STS were 93.1, 96.3 and 97.5%. The limit of detection (LOD) and limit of quantification (LOQ) for STS was 0.1 microg/ml and 0.5 microg/ml, respectively. Linear responses were observed over a wide concentration range (0.5-100 microg/ml) for STS in mouse plasma. STS can be detected after intravenous administration. This method was performed for the first time in pharmacokinetic studies of STS in the mouse.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Fenantrenos/sangue , Animais , Estabilidade de Medicamentos , Masculino , Camundongos , Fenantrenos/farmacocinética , Compostos de Amônio Quaternário/química , Reprodutibilidade dos Testes , Espectrofotometria Ultravioleta
9.
Arch Orthop Trauma Surg ; 121(10): 566-70, 2001 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11768637

RESUMO

It is well-known that 'wound excision' is essential in the primary treatment of wounds in war, particularly thorough debridement of the devitalized tissues around the path of a penetrating projectile. Nowadays, the gunshot wounds in peacetime have become prevalent. Instead of the traditional method of 'wound excision' (excision), we used the method of 'incision and drainage' (incision) in the primary surgery of these gunshot wounds of extremities. To determine the treatment effectiveness of these different surgical methods (incision and excision), two groups of dogs were shot in the proximal part of one hind leg with an American M-16 rifle. One group was treated by the method of 'excision'; in the other group 'incision' were performed. No difference in infection rate was noted between the two groups. Similarly, no difference in bacterial count was found between the two groups during the observation period. Also, there was no difference in healing time; the wounds in both groups had healed by 19.2-21.4 days. Microscopic examination revealed a little normal muscle tissue in the necrotic zone of the incision group which might augment the repair process. These results suggest that there are no differences in the effectiveness in preventing infection between the two methods. 'Incision' might be superior to 'excision' for the management of the gunshot wounds of extremities in peacetime, as it involves a simple operation and there are advantages for tissue healing.


Assuntos
Desbridamento , Drenagem , Traumatismos da Perna/cirurgia , Ferimentos por Arma de Fogo/cirurgia , Animais , Cães , Traumatismos da Perna/microbiologia , Traumatismos da Perna/patologia , Distribuição Aleatória , Irrigação Terapêutica , Ferimentos por Arma de Fogo/microbiologia , Ferimentos por Arma de Fogo/patologia
10.
Zhongguo Zhong Yao Za Zhi ; 26(5): 315-7, 2001 May.
Artigo em Chinês | MEDLINE | ID: mdl-12528519

RESUMO

OBJECTIVE: To prepare stable and redispersable Lyophilization injection of cucurbitacin polylactic acid nanoparticles(Cu-PLA-NP). METHOD: An optimal supporting agent was chosen to prepare the Cu-PLA-NP lyophilization injection. The physical and chemical properties of the injection were evaluated. RESULTS: The shape, diameters, association ratio, drug loading and pH of Cu-PLA-NP colloidal solution and lyophilization injection were not changed. The content of water consisted with the requirement of lyophilization injection. The critical relative humidity was 69.52%. The stability was good. CONCLUSION: It is practicable to prepare Cu-PLA-NP lyophilization injection with proper formulas and preparation processes.


Assuntos
Tecnologia Farmacêutica , Triterpenos , Cucurbitacinas , Portadores de Fármacos , Estabilidade de Medicamentos , Liofilização , Injeções , Ácido Láctico , Poliésteres , Polímeros , Triterpenos/administração & dosagem , Triterpenos/química
11.
Zhongguo Zhong Yao Za Zhi ; 25(2): 91-3, 2000 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-12212078

RESUMO

OBJECTIVE: To predict the stable life for a complex injection of Chinese materia medica. METHOD: The prediction was carried out by initial average rate stability tests, and the contents of puerarin and danshensu (major active components of the injection) were assayed by HPLC. RESULT: The degradation of puerarin followed the first order action, while danshensu was hard to determine; the t0.9,25 degrees C was 3.49 years and 1.69 years respectively. CONCLUSION: The stable life of the injection has been determined as 1.5 years.


Assuntos
Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Combinação de Medicamentos , Estabilidade de Medicamentos , Injeções , Isoflavonas/análise , Lactatos/análise , Pueraria/química , Salvia miltiorrhiza/química
12.
Yao Xue Xue Bao ; 30(8): 615-20, 1995.
Artigo em Chinês | MEDLINE | ID: mdl-8571782

RESUMO

In this paper, the feasibility of skin permeation for lidocaine and pressure sensitive adhesive (PSA) tape formulation containing lidocaine for skin local anesthetic were assessed. Firstly, in vitro skin permeation of the molecular and ionic forms of lidocaine from water and silicone fluid suspensions was measured using a side-by-side two diffusion cells and excised hairless rat skin. Secondly, PSA tape containing lidocaine was prepared by a general casting method using styrene-isoprene-styrene block copolymer. The in vitro release and skin permeation were evaluated and compared with that of Japan marketed xylocaine jelly. The effect of lidocaine concentration on the steady-state flux of skin permeation from 10% to 60% lidocaine PSA tapes was also evaluated.


Assuntos
Anestésicos Locais/farmacocinética , Lidocaína/farmacocinética , Absorção Cutânea , Animais , Masculino , Ratos
13.
Zhongguo Zhong Yao Za Zhi ; 19(1): 25-7, 62, 1994 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-8011118

RESUMO

The contents of bufadienolide and borneol in 01 and 02 Liushen Pills were determined by second-derivative spectrophotometry and gas chromatography respectively. The average recover of bufadienolide by SDS was 100.12% (n = 6, RSD = 1.44%) and of borneol by GC 97.68% (n = 4, RSD = 1.32%).


Assuntos
/análise , Colenos/análise , Materia Medica/química , Bufanolídeos , Cromatografia Gasosa , Espectrofotometria/métodos
14.
J Tongji Med Univ ; 14(3): 162-4, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-7807602

RESUMO

The contraction of isolated rat and rabbit uteri induced by oxytocin and PGF2 alpha was markedly inhibited by chlorpheniramine (Chl) and astemizolum (Ast), both of which also decreased the resting tension of uteri, and their spontaneous contraction. The inhibitory effects of both drugs were dose-dependent. At high concentrations, Chl 7.4 x 10(-4) mol/L and Ast 10(-4) mol/L could counteract the contraction of the uteri induced by Oxy and PGF2 alpha, and their spontaneous contraction as well. They decreased the resting tension to the lower level. The mechanism of their non-special relaxed action on uteri could not be completely explained only by their H1-receptor blocking action. Whether they act by blocking calcium channel or by inhibiting calmodulin (CaM) remains to be further explored.


Assuntos
Astemizol/farmacologia , Clorfeniramina/farmacologia , Contração Uterina/efeitos dos fármacos , Animais , Dinoprostona/antagonistas & inibidores , Feminino , Técnicas In Vitro , Ocitocina/antagonistas & inibidores , Coelhos , Ratos , Ratos Wistar
15.
Yao Xue Xue Bao ; 29(5): 380-6, 1994.
Artigo em Chinês | MEDLINE | ID: mdl-7976354

RESUMO

An optimum procedure was established by orthogonal test for preparing cisplatinum albumin microspheres (CDDP-BSA-MS) with emulsion-chemical cross-linking. The quality, stability, distribution in vivo, kinetic characteristics and safety of the albumin microspheres were studied. The results showed that the surface was regular, the mean size was 13.13 +/- 3.55 microns, embedding ratio was 21.62% and the release characteristics in vitro were in accord with "biphase kinetics equation". The stability of the albumin microspheres was good after three months storage. The microspheres accumulated almost entirely in the lung 15 minutes after intravenous injection to mice. The total amount in the lung was about 97% of the injected dose at the peak concentration. Two-compartmental model can be used to describe the regulation of the pharmacokinetics of albumin microspheres in lung. Observation of the lung slice of mice showed no pathological damage.


Assuntos
Cisplatino/farmacocinética , Imunotoxinas/farmacocinética , Pulmão/metabolismo , Animais , Estabilidade de Medicamentos , Camundongos , Microesferas , Soroalbumina Bovina , Tecnologia Farmacêutica , Distribuição Tecidual
16.
Yao Xue Xue Bao ; 29(7): 544-9, 1994.
Artigo em Chinês | MEDLINE | ID: mdl-7976360

RESUMO

The mitoxantrone polybutylcyanoacrylate nanosparticles (DHAQ-PBCA-NP) were prepared by emulsion polymerization method. The surface charge, drug loading, morphology, size and size distribution, release characteristics in vitro, stability and distribution in animals of DHAQ-PBCA-NP were studied. The results showed that the mean size was d(av) = 55.23 nm, drug loading was 46.77%, embedding ratio was 84.89%. The surface carried negative charge and the release characteristics in vitro was in accord with two phases kinetics law. The colloidal solution of DHAQ-PBCA-NP can undergo boiling for 30 min sterilization. The radioactivity concentrated mainly in liver after iv 3H-DHAQ-PBCA-NP. The radioactivity in liver tumor was higher than that in the liver tissue. DHAQ-PBCA-NP was observed in parenchymal cell 15 min after iv DHAQ-PBCA-NS. This preparation seems to have important value for increasing the anti-liver tumor effect and decreasing the toxicity of DHAQ.


Assuntos
Mitoxantrona/farmacocinética , Animais , Cianoacrilatos , Portadores de Fármacos , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/metabolismo , Camundongos , Camundongos Nus , Microesferas , Mitoxantrona/administração & dosagem , Transplante de Neoplasias
17.
Zhongguo Zhong Yao Za Zhi ; 18(7): 415-8, 447, 1993 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-8267855

RESUMO

01 Liushen Pills (prepared by beta-CD inclusion complex) and 02 Liushen Pills (prepared according to the normal way) were compared by quality inspection, stability test, pharmacological experiments of irritative effects and dissolution test. The results indicated that 01 Liushen Pills were superior to 02 Liushen Pills.


Assuntos
Ciclodextrinas , Medicamentos de Ervas Chinesas , beta-Ciclodextrinas , Adulto , Animais , Combinação de Medicamentos , Composição de Medicamentos , Estabilidade de Medicamentos , Medicamentos de Ervas Chinesas/toxicidade , Feminino , Humanos , Mucosa Intestinal/efeitos dos fármacos , Masculino , Mucosa Bucal/efeitos dos fármacos , Veículos Farmacêuticos , Coelhos , Solubilidade
18.
Zhongguo Zhong Yao Za Zhi ; 18(5): 284-6, 318, 1993 May.
Artigo em Chinês | MEDLINE | ID: mdl-8216802

RESUMO

The preparing process for Sinitang drop pills was optimized by orthogonal test. The results from nine experiments were subjected with three indexes to objective analysis and variance analysis, and an optimum preparing process for Sinitang drop pills was sieved out. The quality of the drop pills prepared in this way was examined to be up to the standard.


Assuntos
Medicamentos de Ervas Chinesas , Formas de Dosagem , Tecnologia Farmacêutica
19.
Yao Xue Xue Bao ; 28(8): 604-8, 1993.
Artigo em Chinês | MEDLINE | ID: mdl-8285069

RESUMO

In this paper the technique of emulsion chemical-crosslinking was used to prepare cisplatin albumin microsphere for jaw squamous cancer by neck external artery embolization. It was yellow powder with yield 80 +/- 5%, mean size 56.3 microns, cisplatin concentration 14.02-14.20%, loading rate 97.08-97.95%. The release characteristics in vitro, sterilization, stability and recipe of disperse solvent of cisplatin albumin microsphere were investigated. Animal test showed that cisplatin albumin microsphere may plug the branches of neck external artery well and remain in local tissue.


Assuntos
Quimioembolização Terapêutica , Cisplatino/administração & dosagem , Sistemas de Liberação de Medicamentos , Neoplasias Maxilomandibulares/terapia , Animais , Artéria Carótida Externa , Cisplatino/farmacocinética , Portadores de Fármacos , Neoplasias Maxilomandibulares/metabolismo , Microesferas , Coelhos , Soroalbumina Bovina
20.
Yao Xue Xue Bao ; 28(1): 68-74, 1993.
Artigo em Chinês | MEDLINE | ID: mdl-8328275

RESUMO

In this paper 6 factors and 12 levels of each variable were selected by Uniform Design Method and computer for preparing albumin microspheres with emulsion-chemical cross-linking. An optimal procedure for preparing albumin microspheres was established and the mean diameter of albumin microspheres is 0.41-0.47 micron. Albumin was labelled with 125I-isotope and 125I-albumin microspheres were prepared according to the optimal procedure. The suspension of 125I-albumin microspheres with 0.1% Tween-80 saline was injected via mice tail vein. The animals were sequentially killed and the radioactivity of blood, spleen, liver, kidney, stomach, heart, lung, thyroid and brain were measured. The results showed that albumin microspheres were accumulated mainly in the liver, about 68% of the injected dose at the peak concentration. The pharmacokinetics of albumin microspheres in liver was also studied and two-compartmental model can be used to describe the regulation.


Assuntos
Antineoplásicos/administração & dosagem , Sistemas de Liberação de Medicamentos , Fígado/metabolismo , Soroalbumina Bovina/farmacocinética , Animais , Portadores de Fármacos , Radioisótopos do Iodo , Camundongos , Microesferas , Tecnologia Farmacêutica/métodos , Distribuição Tecidual
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