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1.
J Low Genit Tract Dis ; 24(1): 38-42, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31860573

RESUMO

OBJECTIVE: The aim of the study was to develop a methodology to identify the best use of a longitudinally measured biomarker in relevance to prognosis. MATERIALS AND METHODS: Data of squamous cell carcinoma antigen (SCC-Ag) from 770 patients with cervical squamous cell carcinoma (SCC) were used. The pretreatment, nadir, and time-dependent SCC-Ag values were analyzed in relevance to disease relapse and death with univariate and multivariate analysis side by side with a variety of available clinicopathologic factors. The predictive power of the significant variates was evaluated by C-index with 5-fold cross validation. RESULTS: The pretreatment, nadir, and time-dependent SCC-Ag were all significant risk factors for both relapse and death in the univariate analysis (p < .05), and the time-dependent SCC-Ag had the highest C-index in both events. The nadir and time-dependent SCC-Ag were both independently significant in response to relapse with International Federation of Gynecology and Obstetrics (FIGO) stage as the covariate, and the latter had a higher C-index (0.745). Only the time-dependent SCC-Ag was independently significant together with FIGO stage in response to death with the C-index at 0.844. CONCLUSIONS: Increases in the serum level of SCC-Ag in cervical SCC patients suggest a higher risk of both relapse and death. The best use of serial SCC-Ag measurements is to include the time-dependent value in prognostic assessment with FIGO stage also accounted for. Cervical SCC patients should be followed up on their levels of SCC-Ag, and prognostic evaluation should be updated with recent measurements.

2.
J Clin Neurosci ; 55: 45-51, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30077473

RESUMO

According to the context updating theory, the oddball P300 component indexes brain activities underlying revision of the mental representation induced by incoming stimuli. It involves an attention-driven comparison process that evaluates the representation of the previous event in working memory. Delayed latencies have been reported for various types of stroke such as unilateral thalamic stroke. We investigated memory updating effects in patients with putamen stroke. Two groups of patients with putamen and thalamic stroke were recruited along with two control groups of young and old healthy participants. Auditory and visual P300 were elicited respectively in a two-stimulus oddball paradigm. The auditory P300 peak latencies were significantly longer in patients with a putamen lesion than in the aged and young control groups and the same pattern was found in the thalamus-lesioned patient. The delayed auditory P300 component in both patient groups but neither control group suggests impairment of memory updating in patients with putamen stroke comparable with thalamic stroke. Our study illustrates the important role of subcortical structures subserved in context updating.


Assuntos
Potencial Evocado P300/fisiologia , Transtornos da Memória/fisiopatologia , Putamen/fisiopatologia , Acidente Vascular Cerebral/fisiopatologia , Adulto , Ondas Encefálicas/fisiologia , Eletroencefalografia , Potenciais Evocados Auditivos/fisiologia , Potenciais Evocados Visuais/fisiologia , Feminino , Humanos , Masculino , Transtornos da Memória/etiologia , Acidente Vascular Cerebral/complicações
3.
Taiwan J Obstet Gynecol ; 57(4): 546-550, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30122576

RESUMO

OBJECTIVE: To determine the association between pregnancy-induced hypertension (PIH) and transient tachypnea of the newborn (TTN) and to identify the predictive risk factors. MATERIALS AND METHODS: Pregnant women with a newly diagnosed PIH (between 2000 and 2013) from the Taiwan National Health Insurance Research Database (NHIRD) were compared with a matched (with respect to age and year of delivery) cohort of pregnant women without PIH. The occurrence of TTN was evaluated in both cohorts. RESULTS: Among the 23.3 million individuals registered in the NHIRD, 29,013 patients with PIH and 116,052 matched controls were identified. According to a multivariate analysis, PIH (odds ratio [OR] = 1.85, 95% confidence interval [CI] = 1.69-2.03, p < 0.0001), age ≥ 30 years (OR = 1.38, 95% CI = 1.26-1.51, p < 0.0001), primiparity (OR = 1.37, 95% CI = 1.24-1.5, p < 0.0001), preterm birth (OR = 3.4, 95% CI = 3.09-3.75, p < 0.0001), multiple births (OR = 2.54, 95% CI = 2.24-2.89, p < 0.0001), and cesarean section (OR = 1.71, 95% CI = 1.56-1.88, p < 0.0001) were independent risk factors for the development of TTN. CONCLUSION: Women with PIH have an increased risk of having infants who develop TTN compared with those without PIH. Additionally, age ≥30 years, primiparity, preterm birth, multiple births, and cesarean section were independent risk factors for the development of TTN.


Assuntos
Hipertensão Induzida pela Gravidez/fisiopatologia , Taquipneia Transitória do Recém-Nascido/epidemiologia , Adulto , Cesárea/efeitos adversos , Estudos de Coortes , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Idade Materna , Razão de Chances , Paridade , Gravidez , Nascimento Prematuro , Fatores de Risco , Taiwan/epidemiologia
4.
Behav Neurol ; 2016: 9287092, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27555668

RESUMO

Because of dopaminergic neurodegeneration, patients with Parkinson's disease (PD) show impairment in the recognition of negative facial expressions. In the present study, we aimed to determine whether PD patients with more advanced motor problems would show a much greater deficit in recognition of emotional facial expressions than a control group and whether impairment of emotion recognition would extend to positive emotions. Twenty-nine PD patients and 29 age-matched healthy controls were recruited. Participants were asked to discriminate emotions in Experiment 1 and identify gender in Experiment 2. In Experiment 1, PD patients demonstrated a recognition deficit for negative (sadness and anger) and positive faces. Further analysis showed that only PD patients with high motor dysfunction performed poorly in recognition of happy faces. In Experiment 2, PD patients showed an intact ability for gender identification, and the results eliminated possible abilities in the functions measured in Experiment 2 as alternative explanations for the results of Experiment 1. We concluded that patients' ability to recognize emotions deteriorated as the disease progressed. Recognition of negative emotions was impaired first, and then the impairment extended to positive emotions.


Assuntos
Doença de Parkinson/psicologia , Idoso , Estudos de Casos e Controles , Emoções/fisiologia , Expressão Facial , Reconhecimento Facial/fisiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Testes Neuropsicológicos , Escalas de Graduação Psiquiátrica , Percepção Social
6.
Food Chem Toxicol ; 67: 161-8, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24582683

RESUMO

Monascus-fermented products, including red mold rice and red mold dioscorea, have been developed as functional foods with many health benefits. We performed safety and mutagenic evaluations on red mold dioscorea powder (RMDP) fermented from Monascus purpureus NTU 568. The results of Ames test using Salmonella typhimurium strains TA97a, TA98, TA100, TA102, and TA1535 showed that RMDP (⩽5 mg/plate) was not mutagenic. The mammalian chromosomal aberration test showed that the number of Chinese hamster ovary cells with abnormal chromosomes was <3% after RMDP treatment (maximum concentration: 5 mg/mL). Imprinting control region mice were used to estimate the genotoxicity of RMDP. Compared with the control, high-dose RMDP administration (2000 mg/kg) did not show significant differences in the number of reticulocytes or the occurrence of micronucleated reticulocytes. A 28-day oral toxicity assay in Sprague-Dawley rats was performed to investigate the no observed adverse effect level of RMDP. Compared with the control, high-dose RMDP administration (2000 mg/kg) caused no toxicological responses such as mortality, variation in body weight, or toxicopathologic lesions. Thus, RMDP from M. purpureus NTU 568 shows no significant mutagenic or toxic effects.


Assuntos
Fermentação , Fungos/metabolismo , Monascus/química , Animais , Células CHO , Aberrações Cromossômicas , Cricetinae , Cricetulus , Testes de Mutagenicidade , Ratos , Ratos Sprague-Dawley
7.
Behav Res Methods ; 46(1): 284-306, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23784009

RESUMO

The morphological constituents of English compounds (e.g., "butter" and "fly" for "butterfly") and two-character Chinese compounds may differ in meaning from the whole word. Subjective differences and ambiguity of transparency make judgments difficult, and a computational alternative based on a general model might be a way to average across subjective differences. In the present study, we propose two approaches based on latent semantic analysis (Landauer & Dumais in Psychological Review 104:211-240, 1997): Model 1 compares the semantic similarity between a compound word and each of its constituents, and Model 2 derives the dominant meaning of a constituent from a clustering analysis of morphological family members (e.g., "butterfingers" or "buttermilk" for "butter"). The proposed models successfully predicted participants' transparency ratings, and we recommend that experimenters use Model 1 for English compounds and Model 2 for Chinese compounds, on the basis of differences in raters' morphological processing in the different writing systems. The dominance of lexical meaning, semantic transparency, and the average similarity between all pairs within a morphological family are provided, and practical applications for future studies are discussed.


Assuntos
Julgamento , Idioma , Modelos Psicológicos , Modelos Estatísticos , Psicolinguística/métodos , Semântica , Adulto , Área Sob a Curva , Grupo com Ancestrais do Continente Asiático , Feminino , Humanos , Valor Preditivo dos Testes , Psicolinguística/estatística & dados numéricos , Curva ROC , Vocabulário
8.
J Agric Food Chem ; 61(11): 2796-802, 2013 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-23458314

RESUMO

The Monascus species has been used in foods for thousands of years in China. In this study, 10 azaphilone pigments, including four yellow and six orange pigments, were isolated from the fermented rice and dioscorea of Monascus purpureus NTU 568. By employing lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells, we determined the inhibitory activities of these pigments on nitric oxide (NO) production. As a result, four orange pigments, monaphilols A-D, showed the highest activities (IC50 = 1.0-3.8 µM), compared with the other two orange pigments, monascorubrin (IC50 > 40 µM) and rubropunctatin (IC50 = 21.2 µM), and the four yellow pigments ankaflavin (IC50 = 21.8 µM), monascin (IC50 = 29.1 µM), monaphilone A (IC50 = 19.3 µM), and monaphilone B (IC50 = 22.6 µM). Using Western blot and ELISA kits, we found that treatments with 30 µM of the yellow pigments and 5 µM of the orange pigments could down-regulate the protein expression of inducible nitric oxide synthase (iNOS) and suppress the production of tumor necrosis factor-α (TNF-α), interleukin-1ß (IL-1ß), and interleukin-6 (IL-6). We also used two animal experiments to evaluate the anti-inflammatory effects of these pigments. In a 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema model, eight of these pigments (0.5 mg/ear) could prevent ear edema against TPA administrations on the ears of BALB/c mice. In an LPS-injection mice model, several of these pigments (10 mg/kg) could inhibit the NO, TNF-α, IL-1ß, and IL-6 levels in the plasma of BALB/c mice. As concluded from the in vitro and in vivo studies, six azaphilonoid pigments, namely, ankaflavin, monaphilone A, and monaphilols A-D, showed high potential to be developed into chemopreventive foods or drugs against inflammation-associated diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Edema/tratamento farmacológico , Monascus/química , Pigmentos Biológicos/administração & dosagem , Animais , Anti-Inflamatórios/metabolismo , Cor , Edema/genética , Edema/imunologia , Humanos , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Monascus/metabolismo , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/imunologia , Pigmentos Biológicos/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia
9.
J Agric Food Chem ; 60(32): 7880-5, 2012 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-22835031

RESUMO

Monascus species have traditionally been used in Asian food, with rice as their fermentation substrate. Red mold rice (RMR) contains citrinin, a nephrotoxic agent capable of exerting oxidative stress and cellular apoptosis. We investigated the components in RMR that could minimize the adverse effects of citrinin. Combining chemical separations and bioactivity assays, we identified an antioxidative component called deferricoprogen (DFC) in the fermented rice of Monascus purpureus NTU 568. The DFC structure was confirmed by nuclear magnetic resonance (NMR) and mass spectra analysis. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging activity of DFC was similar to that of vitamin E. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and flow cytometric analysis showed the effect of DFC and citrinin on cell viability and cell cycle. DFC was found to be protective against the cytotoxicity and cell death induced by citrinin on human embryonic kidney (HEK-293) cells. DFC also demonstrated anti-apoptotic property in preventing citrinin-induced apoptosis.


Assuntos
Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Citrinina/farmacologia , Dicetopiperazinas/farmacologia , Ácidos Hidroxâmicos/farmacologia , Monascus/química , Antioxidantes/isolamento & purificação , Caspases/metabolismo , Citrinina/toxicidade , Dicetopiperazinas/isolamento & purificação , Fermentação , Células HEK293 , Humanos , Ácidos Hidroxâmicos/isolamento & purificação , Nefropatias/induzido quimicamente , Nefropatias/prevenção & controle , Oryza/química , Oryza/microbiologia
10.
Molecules ; 17(1): 664-73, 2012 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-22237681

RESUMO

A new azaphilonidal derivative, monapurpyridine A (MPA), has recently been isolated from the fermented products of Monascus purpureus NTU 568. The structure of MPA was elucidated by nuclear magnetic resonance (1H-NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Biological evaluation revealed that MPA could induce cell death in human breast adenocarcinoma cells MCF-7, and it has no significant toxicity to normal mammary epithelial cells M10. The MTT assay and flow cytometric analysis were employed to investigate cell viability and cell cycle influenced by MPA. Moreover, we used Western blot and caspase activity assay to demonstrate the activation of caspase-3, -8 and -9 resulted from MPA. All evidence supported that MPA was suitable for developing into a chemotherapeutic or chemopreventive agent against breast cancer.


Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Monascus/metabolismo , Piridinas/farmacologia , Adenocarcinoma , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Neoplasias da Mama , Caspases/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Meios de Cultura/química , Ativação Enzimática , Feminino , Fermentação , Humanos , Estrutura Molecular , Oryza/química , Piridinas/química , Piridinas/isolamento & purificação
11.
J Agric Food Chem ; 59(9): 4512-8, 2011 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-21506577

RESUMO

Red mold dioscorea (RMD) is a fermented product of Monascus purpureus NTU 568 using dioscorea as culture substrate. To investigate the bioactive components of RMD, six orange pigments including four new azaphilones with yellow fluorescence, monapilol A-D (1-4), and known monascorubrin (5) and rubropunctatin (6) were isolated and characterized. Structural elucidation of new isolates was based on nuclear magnetic resonance ((1)H NMR, (13)C NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. The structures of monapilols (1-4) were similar to those of monascorubrin (5) and rubropunctatin (6); however, the hydroxyl group (8-OH) in compounds 1-4 substituted for the C-8 carbonyl in compounds 5 and 6. Biological evaluation indicated that compounds 1-4 inhibited nitric oxide (NO) production on lipopolysaccharide-stimulated RAW 264.7 cells. Compounds 1-4 also exhibited antiproliferative activities against human laryngeal carcinoma (HEp-2) and human colon adenocarinoma (WiDr).


Assuntos
Dioscorea/microbiologia , Fermentação , Monascus/metabolismo , Pigmentos Biológicos/metabolismo , Pigmentos Biológicos/farmacologia , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Dioscorea/química , Dioscorea/metabolismo , Fluorescência , Humanos , Estrutura Molecular , Pigmentos Biológicos/química
12.
J Agric Food Chem ; 59(4): 1124-30, 2011 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-21261255

RESUMO

An azaphilonidal derivative monaphilone A (MA) was recently isolated from the fermented products of Monascus purpureus NTU 568 by our laboratory. We report here the exploration of apoptosis-related and anti-inflammatory properties of MA and ankaflavin (AK) by some experiments about inducing death of human laryngeal carcinoma cell line HEp-2 and reducing inflammatory responses on murine macrophage RAW 264.7 cells. We employed a ssDNA enzyme-linked immunosorbent assay (ELISA) kit to investigate the nuclear changes of early apoptosis induced by AK and MA on HEp-2 cells and used a western blot and an enzyme activity assay to demonstrate the activation of caspase-3, caspase-8, and caspase-9 by MA and AK. Our studies revealed that AK and MA may decrease lipopolysaccharide (LPS)-induced inflammatory responses, including nitrite productions and expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells. All evidence support that azaphilonidal derivatives from M. purpureus NTU 568, such as AK and MA, are suitable for the development of chemotherapy or chemopreventive agents.


Assuntos
Anti-Inflamatórios/farmacologia , Antineoplásicos/farmacologia , Benzopiranos/farmacologia , Flavinas/farmacologia , Monascus/química , Animais , Apoptose , Caspases/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , Ciclo-Oxigenase 2/análise , Humanos , Neoplasias Laríngeas , Macrófagos , Camundongos , Óxido Nítrico Sintase Tipo II/análise
13.
Molecules ; 15(11): 7815-24, 2010 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-21060290

RESUMO

Six azaphilonoid derivatives, including two new blue fluorescent monapurfluores A (1) and B (2), two known pyridine-containing molecules, monascopyridines C (3) and D (4), and two known monasfluores A (5) and B (6), were isolated and characterized from red mold rice fermented by Monascus purpureus NTU 568. Structural elucidation of new isolates was based on nuclear magnetic resonance (1H- NMR, 13C-NMR, COSY, HMQC, and HMBC) and other spectroscopic analyses. Bioactivity evaluation indicated that 1-6 possessed anti-inflammatory activities with dose-dependent relationships for lipopolysaccharide (LPS)-induced nitric oxide production. Furthermore, 1-4 also showed moderate antiproliferative effects against human laryngeal carcinoma (HEp-2) (IC(50) = 14.81~20.06 µg/mL) and human colon adenocarcinoma (WiDr) (IC(50) = 12.89~21.14 µg/mL).


Assuntos
Anti-Inflamatórios/farmacologia , Monascus/química , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/isolamento & purificação , Linhagem Celular , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Fermentação , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Espectroscopia de Ressonância Magnética , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Piridinas/química , Piridinas/isolamento & purificação
14.
J Agric Food Chem ; 58(14): 8211-6, 2010 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-20597545

RESUMO

Monascus purpureus NTU 568 was a mutant strain from M. purpureus HM105. The methanol extract of red mold rice fermented by this strain exhibited four major yellow pigment signals on HPLC profile. By repeated chemical chromatography methods, three new azaphilone derivatives, namely, monaphilone A (1), B (2) and C (3), along with the known pigments ankaflavin (4) and monascin (5), were isolated and characterized. Based on spectroscopic analyses, mainly 1D and 2D NMR data, the structures of compounds 1-3 were completely elucidated; in addition, 1-3 were determined to be new azaphilone structures, due to the decrease of carbon monoxide for producing a gamma-lactone ring, compared with other azaphilone derivatives. Biological evaluations showed that monaphilone A (1) and B (2) exhibited an antiproliferative effect against HEp-2 (human laryngeal carcinoma cell line) and WiDr (human colon adenocarcinoma cell line), and none of the five compounds had toxicity to normal human lung cell lines (WI-38 and MRC-5) at 70 muM.


Assuntos
Benzopiranos/farmacologia , Cicloexanonas/farmacologia , Inibidores do Crescimento/farmacologia , Monascus/química , Pigmentos Biológicos/farmacologia , Benzopiranos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Cicloexanonas/isolamento & purificação , Inibidores do Crescimento/isolamento & purificação , Humanos , Pigmentos Biológicos/isolamento & purificação
15.
Vision Res ; 50(17): 1649-55, 2010 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-20580732

RESUMO

After prolonged viewing, a static target among moving non-targets is perceived to repeatedly disappear and reappear. An uncrossed stereoscopic disparity of the target facilitates this Motion-Induced Blindness (MIB). Here we test whether monocular depth cues can affect MIB too, and whether they can also affect perceptual fading in static displays. Experiment 1 reveals an effect of interposition: more MIB when the target appears partially covered by, than when it appears to cover, its surroundings. Experiment 2 shows that the effect is indeed due to interposition and not to the target's contours. Experiment 3 induces depth with the watercolor illusion and replicates Experiment 1. Experiments 4 and 5 replicate Experiments 1 and 3 without the use of motion. Since almost any stimulus contains a monocular depth cue, we conclude that perceived depth affects perceptual fading in almost any stimulus, whether dynamic or static.


Assuntos
Percepção de Profundidade/fisiologia , Ilusões Ópticas/fisiologia , Visão Monocular/fisiologia , Adaptação Fisiológica/fisiologia , Sinais (Psicologia) , Humanos , Percepção de Movimento/fisiologia , Mascaramento Perceptivo/fisiologia , Estimulação Luminosa/métodos
16.
Integr Cancer Ther ; 9(2): 204-12, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20356949

RESUMO

The traditional Chinese medicine, Hong-Qu, also called red mold rice in the United States and Europe, is used for treating blood stasis, a disorder related to hyperlipidemia and atherosclerosis. In addition to improving metabolic syndrome, extracts from Monascus-fermented rice inhibit the proliferation of various cancer cells in vitro and in vivo. The objective was to examine the effect of red mold rice ethanol extract (RMRE) on angiogenesis, invasion, and metastasis during tumor progression. RMRE significantly inhibited the proliferation of SW480 and SW620 human colorectal carcinoma cells in a dose- and time-dependent manner by using the MTT assay. A capillary-like network morphology was observed after the addition of 20 ng/mL vascular endothelial growth factor or SW620 culture-conditional medium, which was not seen after RMRE treatment. Moreover, spontaneous intravasation into Matrigel grafts of SW620 cells from the upper to the lower layers in the chick embryo chorioallantoic membrane (CAM) model was detected by the polymerase chain reaction (PCR) amplification of human Alu genomic DNA from the lower CAMs in the RMRE-untreated group. Neovascularization increased to 75.3% +/- 11.6% by SW620 cells onplant with Matrigel grafts in the CAM model. However, RMRE significantly reduced CAM neovascularization in a dose-dependent manner. Finally, RMRE effectively decreased the activity of matrix metalloproteinase (MMP)-7 as determined by reverse transcription PCR (RT-PCR), Western blotting, and casein zymography assays. In summary, Monascus-fermented products exert a potent effect on tumor growth and activation, suggesting that they may serve as supplementary agents in adjuvant cancer therapy.


Assuntos
Neoplasias Colorretais/tratamento farmacológico , Neovascularização Patológica/tratamento farmacológico , Oryza/química , Extratos Vegetais/farmacologia , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Embrião de Galinha , Membrana Corioalantoide/efeitos dos fármacos , Membrana Corioalantoide/patologia , Neoplasias Colorretais/irrigação sanguínea , Neoplasias Colorretais/patologia , Relação Dose-Resposta a Droga , Fermentação , Humanos , Metaloproteinase 7 da Matriz/efeitos dos fármacos , Metaloproteinase 7 da Matriz/metabolismo , Medicina Tradicional Chinesa/métodos , Monascus/metabolismo , Invasividade Neoplásica/prevenção & controle , Extratos Vegetais/administração & dosagem , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo
17.
J Asian Nat Prod Res ; 11(5): 410-6, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19504383

RESUMO

A new dimeric phenylpropanoid namely podonaka A (1), along with the 13 known compounds including diterpenes (2 and 3), norditerpenes (4 and 5), benzenoids (6-10), steroids (11 and 12), chalcone (13), and megastigmane (14), was isolated from the EtOH extract of Podocarpus nakaii Hayata. The structure of 1 was elucidated on the basis of spectroscopic analysis including 1D and 2D NMR and MS techniques. Biological evaluation showed that norditerpenes, inumakilactone B (4), and podolactone E (5) have potent cytotoxic activities against Daoy, WiDr, KB, and HeLa tumor cell lines. Moreover, low dosage of 5 may induce early apoptosis in KB cells before 12 h.


Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Fenilpropionatos/isolamento & purificação , Fenilpropionatos/farmacologia , Plantas Medicinais/química , Traqueófitas/química , Antineoplásicos Fitogênicos/química , Diterpenos/química , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Células KB , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Fenilpropionatos/química , Casca de Planta/química , Caules de Planta/química , Taiwan
18.
Phytochemistry ; 69(7): 1609-16, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18329676

RESUMO

Six saponins, sapinmusaponin K (1) [hederagenin-3-O-(3-O-acetyl-alpha-L-arabinopyranosyl)-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside], sapinmusaponin L (2) [hederagenin-3-O-(4-O-acetyl-alpha-L-arabinopyranosyl)-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabino-pyranoside], sapinmusaponin M (3) [hederagenin-3-O-(2,3-O-diacetyl-beta-D-xylopyranosyl)-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside], sapinmusaponin N (4) [hederagenin-3-O-(2,4-O-diacetyl-beta-D-xylopyranosyl)-(1-->3)-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside], sapinmusaponin O (5) [3,7,20(S)-trihydroxydammar-24-ene-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-D-glucopyranoside], and sapinmusaponin P (6) [3,7,20(R)-trihydroxydammar-24-ene-3-O-alpha-L-rhamnopyranosyl-(1-->2)-beta-d-glucopyranoside], along with seven known saponins (7-13), were isolated from fruits and the galls of Sapindus mukorossi. Their structures were elucidated by 1D and 2D NMR spectroscopic techniques and acid hydrolysis. Biological evaluation indicated that saponins 1-4 and 7-13 showed moderate cytotoxicity against several human tumor cell lines.


Assuntos
Frutas/química , Sapindus/química , Saponinas/química , Triterpenos/química , Espectroscopia de Ressonância Magnética , Estrutura Molecular
20.
Vision Res ; 46(12): 1973-81, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16376963

RESUMO

Perceptual-filling-in (PFI) and motion-induced-blindness (MIB) are two phenomena of temporary blindness in which, after prolonged viewing, perceptually salient targets repeatedly disappear and reappear, amidst a field of distracters (i.e., non-targets). Past studies have shown that boundary adaptation is important in PFI, and that depth ordering between target and distracter pattern is important in MIB. Here we show that the reverse is also true; that boundary adaptation is important in MIB, and that depth ordering is important in PFI. Results corroborate our earlier conjecture that PFI and MIB are highly related phenomena that share a common underlying mechanism. We argue that this mechanism involves boundary adaptation, but also that the depth effect shows that boundary adaptation can be no more than a sufficient cause of PFI and MIB, and not a necessary one.


Assuntos
Percepção de Movimento/fisiologia , Ilusões Ópticas/fisiologia , Reconhecimento Visual de Modelos/fisiologia , Adaptação Fisiológica/fisiologia , Área de Dependência-Independência , Humanos , Mascaramento Perceptivo/fisiologia , Estimulação Luminosa/métodos , Psicofísica
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