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1.
J Surg Oncol ; 2022 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-35041203

RESUMO

BACKGROUND AND OBJECTIVES: Lung cancer patients slated for surgery are at high risk of venous thromboembolism (VTE). Precise risk assessment is necessary for providing proper thromboprophylaxis and reducing morbidity and mortality of VTE. METHODS: A multicenter, observational, cross-sectional cohort study, involving patients with primary lung cancer undergoing surgery, was carried out from August 2016 to December 2019. All patients were assessed according to the Caprini risk assessment model (RAM) and a modified scoring system incorporating elevated D-dimer and new stratification of surgical time. The endpoint was confirmed VTE or patient discharge. RESULTS: Out of 1205 patients, 87 (7.2%) were diagnosed with VTE. The area under the curve of modified scores for VTE was 0.759, which was larger than that of the original one (0.589) (p < 0.05). By modified Caprini scoring system, a higher score was associated with increased VTE risk (odds ratio [OR], 1.345; 95% confidence interval [CI], 1.197-1.512; p < 0.001), and there was an increased OR of 4.090 (95% CI, 2.472-6.768, p < 0.001) for VTE in high-risk category patients. CONCLUSION: Modified Caprini RAM showed an improved prediction of high-risk patients with an elevated likelihood of postoperative VTE compared to the original one.

2.
Brain Struct Funct ; 2022 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-35028746

RESUMO

The enlargement of ventricular volume is a general trend in the elderly, especially in patients with Alzheimer's disease (AD). Multiple susceptibility loci have been reported to have an increased risk for AD and the morphology of brain structures are affected by the variations in the risk loci. Therefore, we hypothesized that genes contributed significantly to the ventricular surface, and the changes of ventricular surface were associated with the impairment of cognitive functions. After the quality controls (QC) and genotyping, a lateral ventricular segmentation method was employed to obtain the surface features of lateral ventricle. We evaluated the influence of 18 selected AD susceptibility loci on both volume and surface morphology across 410 subjects from Alzheimer's Disease Neuroimaging Initiative (ADNI). Correlations were conducted between radial distance (RD) and Montreal Cognitive Assessment (MoCA) subscales. Only the C allele at the rs744373 loci in BIN1 gene significantly accelerated the atrophy of lateral ventricle, including the anterior horn, body, and temporal horn of left lateral ventricle. No significant effect on lateral ventricle was found at other loci. Our results revealed that most regions of the bilateral ventricular surface were significantly negatively correlated with cognitive scores, particularly in delayed recall. Besides, small areas of surface were negatively correlated with language, orientation, and visuospatial scores. Together, our results indicated that the genetic variation affected the localized areas of lateral ventricular surface, and supported that lateral ventricle was an important brain structure associated with cognition in the elderly.

4.
Sci Data ; 9(1): 18, 2022 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-35058449

RESUMO

Drug Toxicity Signature Generation Center (DToxS) at the Icahn School of Medicine at Mount Sinai is one of the centers for the NIH Library of Integrated Network-Based Cellular Signatures (LINCS) program. Its key aim is to generate proteomic and transcriptomic signatures that can predict cardiotoxic adverse effects of kinase inhibitors approved by the Food and Drug Administration. Towards this goal, high throughput shotgun proteomics experiments (308 cell line/drug combinations +64 control lysates) have been conducted. Using computational network analyses, these proteomic data can be integrated with transcriptomic signatures, generated in tandem, to identify cellular signatures of cardiotoxicity that may predict kinase inhibitor-induced toxicity and enable possible mitigation. Both raw and processed proteomics data have passed several quality control steps and been made publicly available on the PRIDE database. This broad protein kinase inhibitor-stimulated human cardiomyocyte proteomic data and signature set is valuable for prediction of drug toxicities.

5.
J Clin Oncol ; : JCO2101344, 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34995100

RESUMO

PURPOSE: We aimed to evaluate the efficacy and feasibility of patient-reported outcome (PRO)-based symptom management in the early period after lung cancer surgery. METHODS: Before surgery, patients with clinically diagnosed lung cancer were randomly assigned 1:1 to receive postoperative PRO-based symptom management or usual care. All patients reported symptoms on MD Anderson Symptom Inventory-Lung Cancer presurgery, daily postsurgery, and twice a week after discharge for up to 4 weeks via an electronic PRO system. In the intervention group, treating surgeons responded to overthreshold electronic alerts driven by any of the five target symptom scores (score ≥ 4 on a 0-10 scale for pain, fatigue, disturbed sleep, shortness of breath, and coughing). The control group patients received usual care and no alerts were generated. The primary outcome was the number of symptom threshold events (any target symptom with a score of ≥ 4) at discharge. Per-protocol analyses were conducted. RESULTS: Of the 166 participants, 83 were randomly allocated to each group. At discharge, the intervention group reported fewer symptom threshold events than the control group (median [interquartile range], 0 [0-2] v 2 [0-3]; P = .007). At 4 weeks postdischarge, this difference was maintained between the intervention and control groups (median [interquartile range], 0 [0-0] v 0 [0-1]; P = .018). The intervention group had a lower complication rate than the control group (21.5% v 40.6%; P = .019). Surgeons spent a median of 3 minutes managing an alert. CONCLUSION: PRO-based symptom management after lung cancer surgery showed lower symptom burden and fewer complications than usual care for up to 4 weeks postdischarge.

6.
Oncogene ; 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34997217

RESUMO

Despite the establishment of novel therapeutic interventions, multiple myeloma (MM) remains invariably incurable due to development of drug resistance and subsequent relapse, which are attributed to activation of oncogenic pathways such as autophagy. Deubiquitinating enzymes (DUBs) are promising targets to overcome resistance to proteasome inhibitor-based treatment. Ubiquitin-specific protease-12 (USP12) is a DUB with a known prognostic value in several cancers. We found that USP12 protein levels were significantly higher in myeloma patient samples than in non-cancerous human samples. Depletion of USP12 suppressed cell growth and clonogenicity and inhibited autophagy. Mechanistic studies showed that USP12 interacted with, deubiquitylated and stabilized the critical autophagy mediator HMGB1 (high mobility group box-1) protein. Knockdown of USP12 decreased the level of HMGB1 and suppressed HMGB1-mediated autophagy in MM. Furthermore, basal autophagy activity associated with USP12/HMGB1 was elevated in bortezomib (BTZ)-resistant MM cell lines. USP12 depletion, concomitant with a reduced expression of HMGB1, suppressed autophagy and increased the sensitivity of resistant cells to BTZ. Collectively, our findings have identified an important role of the deubiquitylase USP12 in pro-survival autophagy and resultant BTZ resistance in MM by stabilizing HMGB1, suggesting that the USP12/HMGB1 axis might be pursued as a potential diagnostic and therapeutic target in human MM.

7.
Brain Imaging Behav ; 2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-34997426

RESUMO

Entropy is a measurement of brain signal complexity. Studies have found increased/decreased entropy of brain signals in psychiatric patients. There is no consistent conclusion regarding the relationship between the entropy of brain signals and mental illness. Therefore, this meta-analysis aimed to identify consistent abnormalities in the brain signal entropy in patients with different mental illnesses. We conducted a systematic search to collect resting-state functional magnetic resonance imaging (rs-fMRI) studies in patients with psychiatric disorders. This work identified 9 eligible rs-fMRI studies, which included a total of 14 experiments, 67 activation foci, and 1383 subjects. We tested the convergence across their findings by using the activation likelihood estimation method. P-value maps were corrected by using cluster-level family-wise error p < 0.05 and permuting 2000 times. Results showed that patients with different psychiatric disorders shared commonly increased entropy of brain signals in the left inferior and middle frontal gyri, and the right fusiform gyrus, cuneus, precuneus. No shared alterations were found in the subcortical regions and cerebellum in the patient group. Our findings suggested that the increased entropy of brain signals in the cortex, not subcortical regions and cerebellum, might have associations with the pathophysiology across mental illnesses. This meta-analysis study provided the first comprehensive understanding of the abnormality in brain signal complexity across patients with different psychiatric disorders.

8.
Artigo em Inglês | MEDLINE | ID: mdl-35050383

RESUMO

PURPOSE: To develop a model for predicting postoperative axial length (AL) in children undergoing cataract surgery younger than 2 years of age. SETTING: The Eye Hospital of Wenzhou Medical University, Hangzhou, China DESIGN: Retrospective study. METHODS: Children were included only if AL data were available before surgery and at least 1 year after surgery. Eyes were divided into pseudophakic, aphakic, and unaffected eye groups. Variables that could influence axial growth were analyzed and a multivariable generalized estimating equation regression model was developed to predict postoperative AL. RESULTS: 333 eyes from 190 patients were included. We observed a logarithmic linear correlation between age and AL in the unaffected eye group, AL = (2.7924 × log of age in months) + 17.607, R2 = 0.6596. Meanwhile, The GEE model of eyes with cataracts can be written as follows: Postoperative AL = 6.408 + 0.611 × (baseline AL) + 0.007 × (baseline age) - -0.006 (baseline age) × (age at follow-up) - -0.391 × coefficient of surgery. The ages were recorded in months, the ALs were recorded in millimeter. CONCLUSIONS: The assessment of AL is one of the most important parts of successful postoperative management in congenital cataract patients. This study established an AL estimate formula for children aged ≤ 2 years with congenital cataract who underwent cataract surgery. This model theoretically could be used to predict individual future AL for child undergoing cataract surgery.

9.
Int J Biol Macromol ; 201: 389-399, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34998886

RESUMO

In the present study, a purified polysaccharide (named SCP-1, Mw 1.368 × 104 Da) was isolated from Sparassis crispa, and its biological activity was evaluated in an oxidative stress model caused by H2O2 in hippocampal neuronal HT22 cells. SCP-1 was a heteropolysaccharide mainly comprising glucose, galactose, fucose, and mannose in a molar ratio of 52.10: 31.10: 15.04: 1.76. The main backbone of SCP-1 was predominantly composed of (1 â†’ 6)-α-D-Galp, (1 â†’ 6)-ß-D-Glcp, (1 â†’ 3)-ß-D-Glcp, (1 â†’ 2,6)-α-D-Galp and (1 â†’ 3,6)-ß-D-Glcp. The branches, substituted at the O-2 of Gal and O-3 of Glc, contained (1 â†’ 6)-2-OMe-α-D-Galp, (1 â†’ 4)-ß-D-Glcp, (1 â†’ 3)-ß-D-Glcp, and terminated by T-α-L-Fucp and T-ß-D-Glcp. Besides, SCP-1 could effectively protect the HT22 cells against H2O2-induced oxidative injury via decreasing the intracellular reactive oxygen species levels, modulating antioxidant enzymes, and reducing cell apoptosis. The findings suggested that SCP-1 holds a potential to be a natural antioxidant or as a neuroprotective agent.

10.
Anal Chem ; 94(2): 1126-1134, 2022 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-34935356

RESUMO

Semicarbazide (SEM) is a widespread carcinogenic and neurotoxic food contaminant, originating from the metabolite of antibiotic nitrofurazone, which is used in aquaculture, or thermal decomposition byproduct of a flour blowing agent azodicarbonamide. Although optical detection technologies are powerful tools considering the advantages of fast response and visualization detection, there are few optical nanosensors for highly sensitive and visual assays of SEM due to no luminescence response and UV absorbance of SEM. Herein, an upconversion luminescence (UCL)-based nanosensor was designed for visual detection of SEM with high sensitivity and good selectivity. The nanosensor was constructed by combining upconversion nanoparticles (UCNPs) and phosphomolybdic acid (PMA), which was used as the specific recognition element of SEM. The developed nanosensor exhibited selective absorbance enhancement and UCL quenching behavior with the addition of SEM based on the inner filter effect (IFE). Since the change in absorbance translated into an exponential change in the luminescence, the sensitivity of the nanosensor was greatly improved. The nanosensor realized a highly sensitive and visual response to SEM in the linear range of 0.5-16 µM with a low limit of detection of 58 nM. Moreover, satisfactory recovery values ranging from 90 to 112% in spiked real samples indicated the practical applicability of the nanosensor. The nanosensor designed here provides a sensitive and convenient sensing strategy for visual detection of hazardous substances and is expected to develop the upconversion sensing application in food safety.

11.
Org Lett ; 24(1): 147-151, 2022 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-34928157

RESUMO

The intramolecular hydroaminocarbonylation of alkenes is a compelling tool to rapidly access lactam, a privileged motif ubiquitous in natural products, pharmaceuticals, and agrochemicals. However, selective carbonylation to bridged polycyclic lactams with a lactam nitrogen at a bridgehead position is less explored. We herein report a modular palladium-catalyzed approach to perform a tandem hydrocarbonylative lactamization/Diels-Alder cycloaddition reaction with 2-vinyl aryl aldimines, alkenes, and CO, which offers convenient access to furnish the bridged polycyclic lactams in high yields with high selectivities.

12.
Brief Bioinform ; 23(1)2022 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-34953465

RESUMO

Alzheimer's disease (AD) has a strong genetic predisposition. However, its risk genes remain incompletely identified. We developed an Alzheimer's brain gene network-based approach to predict AD-associated genes by leveraging the functional pattern of known AD-associated genes. Our constructed network outperformed existing networks in predicting AD genes. We then systematically validated the predictions using independent genetic, transcriptomic, proteomic data, neuropathological and clinical data. First, top-ranked genes were enriched in AD-associated pathways. Second, using external gene expression data from the Mount Sinai Brain Bank study, we found that the top-ranked genes were significantly associated with neuropathological and clinical traits, including the Consortium to Establish a Registry for Alzheimer's Disease score, Braak stage score and clinical dementia rating. The analysis of Alzheimer's brain single-cell RNA-seq data revealed cell-type-specific association of predicted genes with early pathology of AD. Third, by interrogating proteomic data in the Religious Orders Study and Memory and Aging Project and Baltimore Longitudinal Study of Aging studies, we observed a significant association of protein expression level with cognitive function and AD clinical severity. The network, method and predictions could become a valuable resource to advance the identification of risk genes for AD.

13.
Artigo em Inglês | MEDLINE | ID: mdl-34389436

RESUMO

BACKGROUND: The latest studies have considered the time-dependent structures in dynamic brain networks. However, the effect of periphery structures on the temporal flow of information remains unexplored in patients with major depressive disorder (MDD). In this work, we aimed to explore the pattern of interactions between brain regions in MDD across space and time. METHODS: We concentrated on the temporal reachability of nodes in temporal brain networks derived from the resting-state functional magnetic resonance imaging (rs-fMRI) of 55 MDD patients and 62 sex-, age-matched healthy controls. Specifically, temporal connectedness and temporal efficiency (TEF) were estimated based on the length of temporal paths between node pairs. Subsequently, the temporal clustering coefficient (TCC) and temporal distance were jointly employed to explore the patterns in which a node's periphery structure affects its reachability. RESULTS: Significantly higher TEF and lower TCC were found in temporal brain networks in MDD. Besides, significant between-group differences of nodal TCC were detected in regions of sensory perception systems. Considering the temporal paths that begin or end at these regions, MDD patients showed several altered temporal distances. CONCLUSION: Our results showed that the temporal reachability of specific brain regions in MDD could be affected as their periphery structures evolve, which may explain the dysfunction of sensory perception systems in the spatiotemporal domain.

14.
Chemosphere ; 286(Pt 1): 131648, 2022 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-34315079

RESUMO

Aquatic macrophytes have been widely employed for in-situ phytoremediation of cadmium (Cd) polluted sediments. But, little is known about the responses of rhizosphere bacteria and their interspecific interactions to phytoremediation. In this study, the α-diversity, community composition, co-occurrence network and keystone species of sediment bacteria in rhizosphere zones of two typical macrophytes, Hydrilla verticillata and Elodea canadensis, were investigated using 16S rRNA gene high-throughput sequencing. The results showed that after fifty days of phytoremediation, a group of specialized sediment bacteria were assembled in the rhizosphere zones closely associated with different host macrophytes. Rhizosphere micro-environments, i.e., the increases of redox potential and organic matter and the decreases of pH, nitrogen and phosphorus, reduced bacterial α-diversity through niche-based species-sorting process, which in turn reduced interspecific mutualistic relationships. But meanwhile, benefiting from the nutrients supplied from macrophyte roots, more bacterial species survived in the highly Cd-contaminated sediments (50 mg kg-1). In addition, the co-occurrence network revealed that both macrophytes harbored two same keystone bacteria with the high betweenness centrality values, including the family Pedosphaeraceae (genus_unclassified) and genus Parasegetibacter. Their relative abundances were up to 28-fold and 25-fold higher than other keystone species, respectively. Furthermore, these two keystone bacteria were metabolic generalists with vital ecological functions, which posed significant potentials for promoting plant growth and tolerating Cd bio-toxicity. Therefore, the identified keystone rhizobacteria, Pedosphaeraceae and Parasegetibacter, would be potential microbial modulations applied for the future optimization of phytoremediation in Cd-contaminated sediment.


Assuntos
Hydrocharitaceae , Biodegradação Ambiental , Cádmio/análise , RNA Ribossômico 16S/genética , Rizosfera
15.
Sci Total Environ ; 806(Pt 3): 150467, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-34592285

RESUMO

Photochemical transformation of natural organic matter in aquatic environments strongly impacts the environmental behaviors of carbon, nutrients, and pollutants by affecting their solubility, toxicity, bioavailability, and mobility. However, the role of particulate organic matter (POM) in environmental photogeochemistry has received much less attention than that of dissolved organic matter (DOM). In this study, a systematic overview was conducted to summarize the photodissolution and photoflocculation of POM in aquatic systems. The photodissolution of various POM, such as resuspended sediments and algal detritus, could be a potential and important source of DOM in the overlying waters, and these photoreleased DOM were dominated by humic-like components. The photogeochemistry of POM is thought to proceed via direct photochemical reactions and reactive radical-dominated indirect processes. Photodissolution can modify the bioavailability of organic matter and influence the biogeochemical cycling of nutrients, heavy metals, and organic pollutants. In addition, the photo-induced flocculation of DOM to POM could also influence the transport and transformation of organic matter and its associated pollutants. The photochemistry of POM can be significantly influenced by several environmental factors, including irradiation wavelength and intensity, organic matter properties, and radical oxygen species. POM photogeochemistry is one of the most important components of the global cycling of natural organic matter. Further studies regarding photogeochemistry should be conducted to overcome the potential problems arising from the concurrent photodegradation of organic matter and to further develop more filed investigations and analytical methods.


Assuntos
Material Particulado , Fotólise , Solubilidade , Espectrometria de Fluorescência
16.
Neuroimage ; 247: 118826, 2022 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-34923135

RESUMO

Chunk decomposition, which requires the mental representation transformation in accordance with behavioral goals, is of vital importance to problem solving and creative thinking. Previous studies have identified that the frontal, parietal, and occipital cortex in the cognitive control network selectively activated in response to chunk tightness, however, functional localization strategy may overlook the interaction brain regions. Based on the notion of a global brain network, we proposed that multiple specialized regions have to be interconnected to maintain goal representation during the course of chunk decomposition. Therefore, the present study applied a beta-series correlation method to investigate interregional functional connectivity in the event-related design of chunk decomposition tasks using Chinese characters, which would highlight critical nodes irrespective to chunk tightness. The results reveal a network of functional hubs with highly within or between module connections, including the orbitofrontal cortex, superior/inferior parietal lobule, hippocampus, and thalamus. We speculate that the thalamus integrates information across modular as an integrative hub while the orbitofrontal cortex tracks the mental states of chunk decomposition on a moment-to-moment basis. The superior and inferior parietal lobule collaborate to manipulate the mental representation of chunk decomposition and the hippocampus associates the relationship between elements in the question and solution phase. Furthermore, the tightness of chunks is not only associated with different processors in visual systems but also leads to increased intermodular connections in right superior frontal gyrus and left precentral gyrus. To summary up, the present study first reveals the task-modulated brain network of chunk decomposition in addition to the tightness-related nodes in the frontal and occipital cortex.

17.
J Affect Disord ; 296: 622-634, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34688026

RESUMO

RATIONALE/IMPORTANCE: Researches have highlighted communication deficits between resting-state brain networks in major depressive disorder (MDD), as reflected in abnormal functional connectivity (FC). However, it is unclear whether impaired FC is associated with MDD pathology or is simply incidental to MDD symptoms. Moreover, there is no generalized theory to analyze the impact of treatment modalities on MDD. OBJECTIVES: To address the issues, we conducted a systematic review of 49 eligible papers to provide insight into the pathological mechanisms of MDD patients by summarizing resting-state FC alterations involving mood and cognitive abnormalities and the effects of medications on them. RESULTS: Mood disorders in MDD were characterized by abnormal FC between the amygdala, insula, anterior cingulate cortex (ACC), and prefrontal cortex (PFC). Cognitive impairment manifests as deficits in executive function, attention, memory, and rumination, primarily modulated by dysfunction between the fronto-parietal network and default mode network. Especially, we proposed the set of core abnormal FC (CA-FC) contributing to mood and cognitive impairment in MDD, currently including ACC-left precuneus/amygdala, rostral ACC-left dorsolateral PFC, left subgenual ACC-left cerebellar, left PFC- anterior subcallosal, and left precuneus-left pulvinar. After treatment, patients with normalized CA-FC showed remission of depressive symptoms. CONCLUSIONS: We propose a CA-FC set for possible causative principle of MDD, which unifies the FC results from specific, difficult-to-analyze conditions into one outcome set for screening. Furthermore, CA-FC varies from person to person, and the low success rate of a single treatment may be due to the inability to cover too many CA-FC.

18.
J Phys Chem Lett ; : 11894-11901, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34878274

RESUMO

This paper reports packing-shape effects of amplified spontaneous emission (ASE) through orbital polarization dynamics between light-emitting excitons by stacking perovskite (MAPbBr3) quantum dots (QDs sized between 10 nm and 14 nm) into rod-like and diamond-like aggregates. The rod-like packing shows a prolonged photoluminescence (PL) lifetime (184 ns) with 3 nm red-shifted peak (525 nm) as compared to the diamond-like packing (PL peak, 522 nm; lifetime, 19 ns). This indicates that the rod-like packing forms a stronger interaction between QDs with reduced surface-charged defects, leading to surface-to-inside property-tuning capability with an ASE. Interestingly, the ASE enabled by rod-like packing shows an orbit-orbit polarization interaction between light-emitting excitons, identified by linearly/circularly polarized pumping conditions. More importantly, the polarization dynamics is extended to the order of nanoseconds in the rod-like assembly, determined by the observation that within the ASE lifetime (2.54 ns) the rotating pumping beam polarization direction largely affects the coherent interaction between light-emitting excitons.

19.
PLoS Pathog ; 17(12): e1010071, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34882744

RESUMO

Citrus Huanglongbing (HLB), also known as citrus greening, is one of the most devastating citrus diseases worldwide. Candidatus Liberibacter asiaticus (CLas) is the most prevalent strain associated with HLB, which is yet to be cultured in vitro. None of the commercial citrus cultivars are resistant to HLB. The pathosystem of Ca. Liberibacter is complex and remains a mystery. In this review, we focus on the recent progress in genomic research on the pathogen, the interaction of host and CLas, and the influence of CLas infection on the transcripts, proteins, and metabolism of the host. We have also focused on the identification of candidate genes for CLas pathogenicity or the improvements of HLB tolerance in citrus. In the end, we propose potentially promising areas for mechanistic studies of CLas pathogenicity, defense regulators, and genetic improvement for HLB tolerance/resistance in the future.

20.
J Sep Sci ; 2021 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-34882983

RESUMO

By monomer-mediated in-situ growth synthesis strategy, with hydroquinone and 1,3,5-tris(4-aminophenyl)benzene as monomers, a core-shell magnetic porous organic polymer was synthesized through simple azo reaction. Based on this, a magnetic solid-phase extraction-HPLC-fluorescence detection method was proposed for the analysis of fluoroquinolones in honey sample. With ofloxacin, ciprofloxacin, enrofloxacin, lomefloxacin, and difloxacin as target analytes, factors affecting the extraction efficiency have been optimized. The LODs were 1.5-5.4 ng/L (corresponding to 0.23-0.81 µg/kg in honey). The linear range was 0.005-20 µg/L for difloxacin, 0.01-20 µg/L for ofloxacin, ciprofloxacin and lomefloxacin, and 0.02-20 µg/L for enrofloxacin. The enrichment factor is 84.4-91.7-fold with high extraction efficiency of 84.4-91.7%. The method was assessed by the analysis of target fluoroquinolones in honey samples, and the recoveries for the spiked samples were 79.3-95.8%. The results indicate that the established magnetic solid-phase extraction -HPLC-fluorescence detection method is efficient for the analysis of trace fluoroquinolones in honey. This article is protected by copyright. All rights reserved.

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