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1.
Cephalalgia ; : 333102421997850, 2021 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-33631964

RESUMO

OBJECTIVE: To observe the prevalence and characteristics of premonitory symptoms in Chinese migraineurs and explore their associations with migraine-related factors. METHOD: Migraineurs who visited a tertiary headache clinic and one of nine neurology clinics between May 2014 and November 2019 were studied. RESULT: Among the 4821 patients meeting the migraine criteria (International Classification of Headache Disorders, 3rd edition), 1038 (21.5%) patients experienced at least one premonitory symptom. The most common premonitory symptoms were neck stiffness, dizziness, yawning and drowsiness. The logistic regression analysis demonstrated that aura, photophobia, aggravation by routine physical activity, triggers, family history, depression, coffee consumption and physical exercise were associated with an increased probability of experiencing premonitory symptoms (p ≤ 0.001). The premonitory symptoms of migraine with and without aura differ in prevalence and most common symptoms. The cluster analysis revealed pairwise clustering of the following premonitory symptoms: Photophobia/phonophobia, concentration change/dysesthesia, loquacity/overactivity, yawning/drowsiness, fatigue/dizziness, and mood change/irritability. The correlation analysis of triggers and premonitory symptoms revealed that temperature change, environment change, sleep disorder, activity and stress were related to multiple premonitory symptoms, and that food, light, menstruation, alcohol and odor were related to special premonitory symptoms (p ≤ 0.001). CONCLUSION: The prevalence of premonitory symptoms among migraineurs in China is 21.5%. Some factors influence the probability of experiencing premonitory symptoms. Paired premonitory symptoms in the clustering analysis may share similar origins. Certain triggers associated with multiple premonitory symptoms may induce brain dysfunction; however, other triggers that overlap with corresponding special premonitory symptoms may be premonitory symptoms or a form of premonitory symptom.

2.
Clin Transl Gastroenterol ; 11(11): e00262, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33259161

RESUMO

OBJECTIVES: To establish and verify a simple noninvasive model based on the left gastric vein (LGV) to predict the grade of esophageal varices (EV) and high-risk EV (HEV), to facilitate clinical follow-up and timely treatment. METHODS: We enrolled 320 patients with B-viral cirrhosis. All patients underwent endoscopy, laboratory tests, liver and spleen stiffness (SS), and ultrasonography. HEV were analyzed using the χ test/t test and logistic regression in the univariate and multivariate analyses, respectively. EV grades were analyzed using the variance/rank-sum test and logistic regression. A prediction model was derived from the multivariate predictors. RESULTS: In the training set, multivariate analysis showed that the independent factors of different EV grades were SS, LGV diameter, and platelet count (PLT). We developed the LGV diameter-SS to PLT ratio index (LSPI) and LGV diameter/PLT models without SS. The area under the receiver operating characteristic curve of the LSPI for diagnosis of small EV, medium EV, large EV, and HEV was 0.897, 0.899, 0.853, and 0.954, respectively, and that of the LGV/PLT was 0.882, 0.890, 0.837, and 0.942, respectively. For the diagnosis of HEV, the negative predictive value was 94.07% when LSPI < 19.8 and the positive predictive value was 91.49% when LSPI > 23.0. The negative predictive value was 95.92% when LGV/PLT < 5.15, and the positive predictive value was 86.27% when LGV/PLT > 7.40. The predicted values showed similar accuracy in the validation set. DISCUSSION: Under appropriate conditions, the LSPI was an accurate method to detect the grade of EV and HEV. Alternatively, the LGV/PLT may also be useful in diagnosing the varices when condition limited.

3.
Huan Jing Ke Xue ; 41(7): 3066-3075, 2020 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-32608878

RESUMO

Volatile organic compounds (VOCs) were collected at three environmental sampling sites in Yangquan and quantified by gas chromatography-mass selective detector/flame ionization detector(GC-MSD/FID). The VOC sources were identified by diagnostic ratios and positive matrix factorization (PMF), and environmental impact of VOCs on O3 and secondary organic aerosol (SOA) were evaluated. The results showed that the average VOC concentration was (82.1±22.7) µg·m-3, with alkanes being the most abundant group (51.8%), followed by aromatics (17.8%), alkenes (8.0%), and alkynes (3.8%). The diurnal variation of VOCs exhibited a bimodal trend, with twin peaks appearing at 08:00-10:00 and 18:00-20:00, falling to a valley at 12:00-14:00. The results for benzene/toluene (2.1±1.3) and isopentane/n-pentane (1.7±0.6) showed that the ambient VOCs may be influenced by coal combustion and vehicular emissions. Six sources were extracted by PMF:coal combustion (34.9%), vehicle emissions (18.2%), gasoline evaporation (15.2%), industrial emissions (13.6%), biogenic emissions (9.2%), and solvent usage (9.0%). The average concentration of ozone formation potential (OFP) was 156.6 µg·m-3, with the highest contribution from alkenes, while the average concentration of secondary organic aerosol formation potential (SOAp) was 68.7 µg·m-3, mainly from aromatics (93.4%). In summary, coal combustion was the most abundant source of VOCs, and accelerating the management of coal gangue and energy structure readjustment are the key points to address. Meanwhile, restricting the VOCs from vehicle emissions, gasoline evaporation, and industrial emissions is also required.

4.
BMJ Open ; 10(7): e035289, 2020 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-32616490

RESUMO

OBJECTIVE: Many studies have demonstrated that elevated serum uric acid (SUA) level is linked with metabolic syndrome (MetS). However, whether there is a sex difference in the association between SUA and MetS has not been determined. This study aimed to accurately explore the impact of SUA longitudinal changes on MetS by sex. DESIGN: A prospective cohort study. SETTING: The Health Check-up Centre of the Second Hospital affiliated with Dalian Medical University from 2010 to 2016. PARTICIPANTS: A health check-up cohort of 577 men and 1698 women aged 20-60 years who did not exhibit MetS or hyperuricaemia at baseline and underwent at least two physical examinations from 2010 to 2016. PRIMARY AND SECONDARY OUTCOME MEASURES: Weight, height, blood pressure and blood biochemistry parameters, including SUA, were measured. MetS was defined according to the Joint Interim Statement criteria. METHODS: Based on longitudinal data, a linear mixed-effects model was constructed to explore the characteristics of SUA dynamic changes in males and females, and joint modelling of longitudinal and survival data was done to analyse the association between SUA dynamic changes and MetS occurrence. RESULTS: The natural logarithm of SUA (LNSUA) in females exhibited a gradually increasing trend, and its annual growth rate in females who developed MetS was greater than that of the non-MetS females. The longitudinal growth of SUA in females was a risk factor for the onset of MetS, and the estimated HR was 13.2580 (95% CI 1.9106 to 91.9957) for each 1-unit rise in LNSUA longitudinally. An association between the longitudinal growth of LNSUA and MetS was not found in males. CONCLUSIONS: The longitudinal increase in SUA in females could increase the risk of MetS, even if the SUA changes within the normal range. The longitudinal increase in SUA in males was not a predictor for MetS.

5.
Transbound Emerg Dis ; 67(6): 2830-2838, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32469157

RESUMO

Outbreaks of gosling gout have occurred in China since 2017 and caused a considerable economic impact on the poultry industry. While gosling astrovirus (GoAstV) is believed to be the main causal pathogen of gout, the full-blown disease of gout cannot be well reproduced by infecting the goslings with GoAstV, suggesting the possibility of other infectious agents being involved with the development of gosling gout. To assess other possible infectious agents, we collected tissues from gout-affected goslings in 12 goose farms in China, followed by PCR detection of GoAstV, goose reovirus (GRV), goose parvovirus (GPV), fowl adenovirus (FAdV), goose circovirus (GcoV), Tembusu virus (TMUV) and goose haemorrhagic polyomavirus (GHPV). Our data showed that all gout-affected goslings carried both of GoAstV and GPV determined by PCRs, and this was further confirmed by fluorescence multiplex immunohistochemical staining, and phylogenetic analysis of ORF2 gene of GoAstV and VP3 gene of GPV. In addition to the haemorrhage in the kidney, liver, spleen and lung of the gout-affected goslings, histological examinations showed also extensive infiltration of heterophil myelocytes in the kidney, liver, spleen, bursa of Fabricius, thymus, lungs and pancreas. Our findings strongly suggest that coinfection of GoAstV and GPV increases the severity of gout. While this is the first study to report GPV in gout-affected goslings, further studies including infection model are warranted to investigate the role of GPV and its coinfection with GoAstV in the development of gosling gout.

6.
Mol Immunol ; 122: 49-53, 2020 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-32298874

RESUMO

Chicken surfactant protein A (cSP-A) and chicken lung lectin (cLL) are C-type lectins that play important roles in pulmonary host defense responses. Herein, we explored the localization of cSP-A and cLL in the chicken respiratory system. Six tissues from 30-days-old SPF chickens were used to quantify the expression of cSP-A and cLL using the quantitative real-time reverse transcriptional polymerase chain reaction (qRT-PCR) and fluorescence multiplex immunohistochemistry staining (fluorescence mIHC staining). Results showed that cSP-A and cLL mRNA were highly expressed in lungs compared to other tissues. cSP-A mRNA expression levels in all tissues were higher compared with cLL expression levels as analyzed using qRT-PCR. Fluorescence mIHC co-expression of cSP-A and cLL were mainly detected in lung parabronchial epithelia, and mucosal epithelia of larynx, trachea, syrinx, bronchus and air sac, with cSP-A showing a stronger positive staining compared with cLL. cLL is expressed on both mucosal surfaces, some individual lung epithelial cells and cartilage cells, while cSP-A is mainly restricted to mucosal surfaces of the respiratory tract. These histological findings may be useful for understanding the biological significance of this pulmonary lectins in future studies.

7.
Arch Gynecol Obstet ; 301(5): 1307-1315, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32303890

RESUMO

BACKGROUND: MicroRNAs (miRNAs) have been increasingly exploited in human malignancies. The regulation of microRNA-455-5p (miR-455-5p) has been shown in several cancers, except for cervical carcinoma. Therefore, the role of miR-455-5p was exploited in cervical carcinoma. METHODS: The qRT-PCR experiment was used to assess miR-455-5p and S1PR1 expression levels. We explored the function of miR-455-5p through MTT and Transwell assays. The mTOR pathway and cell apoptosis were detected by Western blot assays. The relationship between miR-455-5p and S1PR1 was testified by dual-luciferase reporter assay. RESULTS: MiR-455-5p expression was decreased in cervical carcinoma, which was related to poor clinical outcome in cervical carcinoma patients. MiR-455-5p inhibited cell viability and metastasis in cervical carcinoma. Further, S1PR1 is a direct target of miR-455-5p. S1PR1 recovered the inhibition of cell viability and metastasis induced by miR-455-5p in cervical carcinoma. In addition, miR-455-5p induced cell apoptosis and inactivated the mTOR pathway in cervical carcinoma. CONCLUSION: MiR-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking the mTOR pathway.


Assuntos
MicroRNAs/metabolismo , Receptores de Esfingosina-1-Fosfato/genética , Serina-Treonina Quinases TOR/genética , Neoplasias do Colo do Útero/genética , Linhagem Celular Tumoral , Proliferação de Células , Feminino , Humanos , Transfecção , Neoplasias do Colo do Útero/patologia
8.
Dalton Trans ; 49(3): 719-728, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31850462

RESUMO

Zero thermal expansion (ZTE) and zero linear compressibility (ZLC) are unique and rare properties. Materials combining ZTE and ZLC will have promising prospects. A novel route is proposed in this work to design the coexistence of uniaxial-ZTE and ZLC based on layered hybrid semiconductors [ZnTe(L)0.5] [L = N2H4, ethylenediamine (en), propyldiamine (pda)]. In the framework of [ZnTe(L)0.5], the organic chain contains the attractive and repulsive interactions that arise from the different organic components. It is demonstrated that changing the length of the organic chain can effectively regulate the interaction between different organic components and then achieve the uniaxial-ZTE and ZLC or the desired thermal expansion and compression behaviors. The origin of the coexistence of abnormal axial responses has been traced from thermodynamic formalisms, model Grüneisen parameters and specific vibration modes. It is found that low-energy phonons play an important internal role in realizing the multi-peculiar properties.

9.
Avian Dis ; 63(4): 731-736, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31865690

RESUMO

Goose astrovirus is a novel and distinct astrovirus that causes fatal visceral gout in 4- to 21-day-old goslings. Goose parvovirus is the etiologic agent of Derzsy disease, an acute, contagious, and fatal disease that affects mainly young goslings. This paper describes the clinical signs and gross and histopathologic features of co-infection with astrovirus and goose parvovirus. Clinical signs and history included increased mortality, depression, anorexia, enteritis, joint swelling, and paralysis. Postmortem examination showed a considerable amount of urate covering the internal organs, especially the heart, liver, and kidney. Some goslings had swollen duodenum and ileum. Histologic lesions in the kidney, liver, spleen, lung, proventriculus, and brain included hemorrhage, congestion, edema, cell necrosis, inflammatory cell infiltration, and an eosinophilic protein-like substance in renal tubules. The extensive infiltration of heterophil myelocytes into the kidney, spleen, liver, lung, bursa of Fabricius, and pancreas is a new finding.


Assuntos
Infecções por Astroviridae/veterinária , Coinfecção/veterinária , Gansos , Infecções por Parvoviridae/veterinária , Doenças das Aves Domésticas/diagnóstico , Animais , Astroviridae/isolamento & purificação , Infecções por Astroviridae/diagnóstico , Infecções por Astroviridae/virologia , China , Coinfecção/diagnóstico , Coinfecção/virologia , Infecções por Parvoviridae/diagnóstico , Infecções por Parvoviridae/virologia , Parvovirinae/isolamento & purificação , Doenças das Aves Domésticas/virologia
10.
BMC Infect Dis ; 19(1): 907, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31664922

RESUMO

BACKGROUND: The objectives of this study were to examine nutrient intakes of tuberculosis (TB) patients and to identify their associated factors. METHODS: In this cross-sectional study, 300 adult TB patients were surveyed in two impoverished counties in China. Nutrient intakes were evaluated through two consecutive 24-h dietary recalls and compared with the Chinese Dietary Reference Intakes (DRIs) 2013. The potential socio-demographic and behavioral factors were analyzed using multivariate logistic model to identify strong influential factors. RESULTS: We found that mean daily energy intake was 1655.0 kcal (SD: 619.3 kcal) and 1360.3 kcal (SD: 552.1 kcal) for male and female patients, respectively. The mean daily energy intake was significantly lower than that has been recommended by DRI (i.e., 2250 and 1800 kcal for males and females, respectively), with 87.4% of the male patients and 59.9% of female patients failed to consume adequate energy. The protein intakes were 44.6 g (SD: 18.2 g) and 35.9 g (SD: 12.3 g) for male and female patients, respectively, which were lower than the recommended values by DRI (i.e., 65 and 55 g for males and females, respectively). Most male (90.8%) and female (58.4%) TB patients had insufficient daily protein intake. Further analyses suggested that mean daily intakes of many micronutrients, were insufficient, while for most of patients, intakes of vitamin E and sodium were sufficient. We identified that unemployment was a risk factor for low energy intake (p < 0.05) and out-home-eating was a protective factor for low protein intake (p < 0.01). CONCLUSIONS: In impoverished areas in China, intakes of macronutrients and most micronutrients in TB patients were inadequate compared with DRIs, especially for unemployed patients and patients eating at home. These findings suggested that public health actions are needed to promote education on TB patients about significance of nutritional support, and, further interventions in TB patients' nutritional intakes are also required.


Assuntos
Ingestão de Energia , Comportamento Alimentar , Fatores Socioeconômicos , Tuberculose/epidemiologia , Adolescente , Adulto , Idoso , Conscientização , China/epidemiologia , Estudos Transversais , Dieta , Feminino , Humanos , Masculino , Desnutrição , Micronutrientes , Pessoa de Meia-Idade , Educação de Pacientes como Assunto , Saúde Pública , Recomendações Nutricionais , Desemprego , Adulto Jovem
11.
Nanoscale ; 11(29): 13909-13916, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31304941

RESUMO

Mechanically strong carbon nanotube (CNT) fibers have increasingly become the focus of the present research in the fiber industry. However, the weak or even a lack of interconnections between adjacent CNTs induces much inter-tube slippages during fiber failure, and thus results in their low mechanical strength. Moreover, achieving fast cross-linking between neighbouring CNTs on a large scale to prevent the failure by slip is still a big challenge. Herein we report an ultrafast and continuous tension-annealing process to achieve the considerably improved tube alignment and strong covalent cross-linking of neighbouring CNTs in milliseconds, resulting in great improvement of the fiber performance. The CNT fibers were heated to high temperature (∼2450 °C) by Joule heating under the applied tension and subsequently annealed for just 12 ms. Due to the rapid electromechanical response of the fibers, instant nanotube rearrangements coupled by the formation of cross-links robustly bonding the adjacent CNTs occurred at power-on, which could be attributed to the considerable increases of strength and modulus by factors of 2.9 (up to 3.2 GPa) and 4.8 (up to 123 GPa), respectively. The resultant fibers showed high specific strength (2.2 N per tex), comparable with that of PAN-based carbon fibers, and high specific electrical conductivity higher than that of PAN-based carbon fibers. Moreover, the obtained strongly crosslinked and highly dense structures also endowed the fibers with the significantly improved thermal stability under a high-temperature oxidation atmosphere. Moreover, a continuous tension-annealing process was designed to achieve the large scale production of high performance fibers with the average strength of 2.2 GPa. The high-toughness, lightweight and continuous features together with their outstanding mechanical and electrical properties would certainly boost the large-scale applications of CNT fibers.

12.
Res Vet Sci ; 125: 212-217, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31271953

RESUMO

Goose parvovirus (GPV) is the etiological agent of Derzsy's disease, with a natural reservoir consisting only of geese and Muscovy ducks. However, the pathological changes in the immune organs of ducklings experimentally infected with GPV remain unknown. In this study, 2-day-old Cherry Valley ducklings were intramuscularly injected with GPV. Immune organs (e.g., thymus, bursa of Fabricius, spleen, Harderian gland, cecal tonsil, bone marrow, and peripheral blood lymphocytes [PBLs]) were collected 1, 3, 5, 7, 10, and 14 days post-infection (dpi). Pathological lesions were assessed by histology and the viral load was concurrently assessed using quantitative real-time polymerase chain reaction. GPV antigen was detected via immunofluorescence staining and immunohistochemistry. No clinical symptoms or death were observed in the infected ducklings from 1 to 14 dpi; however, lesions with different degrees of hemorrhage and hyperemia were observed in the thymus, spleen and Harderian gland. Lymphocyte necrosis was identified in the thymus and spleen. In the immune organs, the highest viral loads were found in the spleen at 7 dpi, followed by the bone marrow, PBLs, and cecal tonsil at 3 dpi, and the bursa, Harderian gland, and thymus at 1 dpi. GPV antigen was primarily expressed in the cecal tonsil, spleen, and Harderian gland at 5 dpi, as well as in the PBLs and bone marrow at 3 dpi. Our findings indicate widespread GPV replication and dissemination in the immune organs of Cherry Valley ducklings.


Assuntos
Bolsa de Fabricius/patologia , Patos , Infecções por Parvoviridae/veterinária , Parvovirinae , Doenças das Aves Domésticas/virologia , Baço/patologia , Animais , Bolsa de Fabricius/virologia , Gansos , Infecções por Parvoviridae/patologia , Parvovirus/genética , Doenças das Aves Domésticas/patologia , Reação em Cadeia da Polimerase em Tempo Real , Baço/virologia , Carga Viral
13.
Child Obes ; 15(5): 306-312, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-31058538

RESUMO

Background: Childhood obesity is a recognized public health concern worldwide. It is essential to study the natural progression of obesity in the interest of prevention. This study aimed to describe the dynamic changes in weight status among elementary school children and identify the significant factors influencing the progression or regression of weight status. Methods: This study involved 928 elementary school children who were followed up annually during their elementary school years. Heights, weights, and vital capacity (VC) were measured each school year. A multistate Markov model containing three weight states was fit to longitudinal weight status data. Results: Children with healthy weight and obesity tended to stay in their preceding weight state. Children with overweight, in contrast, were more likely to move to the other two states. The mean sojourn time in obesity and in overweight states was 5.15 years (95% confidence interval [CI]: 4.22-6.3) and 2 years (95% CI: 1.76-2.28), respectively. Children in lower grades, those with a lower VC index, those with a higher initial BMI, those with a higher annual weight increment, and boys were at increased risk of progression to overweight or obesity, with a decreased probability of regression. Conclusions: Children with obesity were more resistant to recovery than those with overweight. Prevention and intervention measures should be adopted early when abnormal weight onset occurs. The multistate Markov model was an advanced tool to analyze dynamic changes in status and identify significant factors for progression and regression and helped to develop prevention and intervention targeting strategies.


Assuntos
Peso Corporal/fisiologia , Obesidade Pediátrica/epidemiologia , Estatura/fisiologia , Criança , Feminino , Humanos , Estudos Longitudinais , Masculino , Cadeias de Markov , Estudantes/estatística & dados numéricos , Capacidade Vital/fisiologia , Ganho de Peso/fisiologia
14.
Oxid Med Cell Longev ; 2018: 1526125, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30538798

RESUMO

The main purposes of this study were to screen the antioxidant activities of various fractions of Hemerocallis citrina Baroni and test their hepatoprotective effects in vitro. Antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing power experiments) and tert-butyl hydroperoxide- (t-BHP-) induced BRL-3A oxidative damage experiments were performed in vitro. The H. citrina ethyl acetate fraction (HCEA) was determined to have strong antioxidant activity because of its high flavonoid and polyphenol content. Ultraperformance liquid chromatography- (UPLC-) photodiode array (PDA)/mass spectrometry (MS) analysis showed that the main components of the HCEA were flavonoids and caffeic acid derivatives. A total of 17 compounds were identified. HCEA also effectively protected the liver against t-BHP-induced oxidative stress injury and significantly reduced reactive oxygen (ROS) accumulation. Moreover, HCEA significantly reduced levels of alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH). Further studies have shown that HCEA inhibits t-BHP-induced apoptosis by increasing B-cell lymphoma-2 (BCL-2) activity and decreasing caspase-3 and caspase-9 activity. Moreover, HCEA enhanced the activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the total antioxidant capacity (T-AOC), and increased the antioxidant level of glutathione (GSH) in BRL-3A cells. HCEA increased the antioxidant capacity of cells by increasing the gene expression of AMP-activated protein kinase (AMPK), extracellular signal-regulated kinase (ERK), P38, nuclear factor, erythroid 2 like 2 (Nrf2), SOD, glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and heme oxygenase 1 (HO-1), which are associated with antioxidant pathways to protect against oxidative stress. In conclusion, HCEA protected BRL-3A cells against t-BHP-induced oxidative stress damage via antioxidant and antiapoptosis pathways. Therefore, H. citrina Baroni may serve as a potential hepatoprotective drug.


Assuntos
Antioxidantes/farmacologia , Hemerocallis , Hepatócitos/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Acetatos/farmacologia , Animais , Antioxidantes/química , Apoptose/efeitos dos fármacos , Linhagem Celular , Hemerocallis/química , Hepatócitos/metabolismo , Peróxido de Hidrogênio/toxicidade , Extratos Vegetais/química , Substâncias Protetoras/farmacologia , Ratos , terc-Butil Hidroperóxido/toxicidade
15.
Adv Mater ; 30(43): e1804944, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30256476

RESUMO

Flexible and stretchable physical sensors capable of both energy harvesting and self-powered sensing are vital to the rapid advancements in wearable electronics. Even so, there exist few studies that can integrate energy harvesting and self-powered sensing into a single electronic skin. Here, a stretchable and washable skin-inspired triboelectric nanogenerator (SI-TENG) is developed for both biomechanical energy harvesting and versatile pressure sensing. A planar and designable conductive yarn network constructed from a three-ply-twisted silver-coated nylon yarn is embedded into flexible elastomer, endowing the SI-TENG with desired stretchability, good sensitivity, high detection precision, fast responsivity, and excellent mechanical stability. With a maximum average power density of 230 mW m-2 , the SI-TENG is able to light up 170 light-emitting diodes, charge various capacitors, and drive miniature electronic products. As a self-powered multifunctional sensor, the SI-TENG is adopted to monitor human physiological signals, such as arterial pulse and voice vibrations. Furthermore, an intelligent prosthetic hand, a self-powered pedometer/speedometer, a flexible digital keyboard, and a proof-of-concept pressure-sensor array with 8 × 8 sensing pixels are successively demonstrated to further confirm its versatile application prospects. Based on these merits, the developed SI-TENG has promising applications in wearable powering technology, physiological monitoring, intelligent prostheses, and human-machine interfaces.


Assuntos
Fontes de Energia Bioelétrica , Nanoestruturas , Nylons , Dispositivos Eletrônicos Vestíveis , Elasticidade , Desenho de Equipamento , Mãos , Humanos , Monitorização Fisiológica/instrumentação , Nanoestruturas/química , Nanotecnologia , Nylons/química , Pressão , Próteses e Implantes , Compostos de Prata/química , Pele
16.
J Occup Med Toxicol ; 13: 11, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29560021

RESUMO

Background: Health care workers are at high risk for tuberculosis (TB). China, a high burden TB country, has no policy on medical surveillance for TB among healthcare workers. In this paper, we evaluate whether China's national TB diagnostic guidelines could be used as a framework to screen healthcare workers for pulmonary TB disease in a clinical setting in China. Methods: Between April-August 2010, healthcare workers from 28 facilities in Inner Mongolia Autonomous Region, China were eligible for TB screening, comprised of symptom check, chest X-ray and tuberculin skin testing. Healthcare workers were categorized as having presumptive, confirmed, or clinically-diagnosed pulmonary TB, using Chinese national guidelines. Results: All healthcare workers (N=4347) were eligible for TB screening, of which 4285 (99%) participated in at least one TB screening test. Of the healthcare workers screened, 2% had cough for ≥ 14 days, 3% had a chest X-ray consistent with TB, and 10% had a tuberculin skin test induration ≥ 20 mm. Of these, 124 healthcare workers were identified with presumptive TB (i.e., cough for ≥ 14 days in the past 4 weeks or x-ray consistent with TB). Twelve healthcare workers met the case definition for clinically-diagnosed pulmonary TB, but none were diagnosed with TB during the study period. Conclusion: A substantial proportion of healthcare workers in Inner Mongolia had signs, symptoms, or test results suggestive of TB disease that could have been identified using national TB diagnostic guidelines as a screening framework. However, achieving medical surveillance in China will require a framework that increases the ease, accuracy, and acceptance of TB screening in the medical community. Routine screening with improved diagnostics should be considered to detect tuberculosis disease among healthcare workers and reduce transmission in health care settings in China.

17.
Nanoscale ; 10(8): 4077-4084, 2018 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-29431840

RESUMO

Hybrid smart actuators fabricated using composites of carbon fibers and shape memory polymers have been extensively studied in recent years. However, relatively slow shape recovery has combined with the reset of shape deformation during cycles to restrict their practical use. An electrothermally reversible actuator based on carbon nanotube (CNT) composite yarn containing CNT fiber and thermoplastic polyurethane (TPU) resin with excellent shape memory was investigated in this paper. The combination of CNT yarn and TPU resin considerably amplified the contraction and stability. Large tensile stroke was obtained within 5 s (∼13.8%) while lifting a load that was ∼1905 times as heavy as the actuator. The generated contractive stress reached more than 33 MPa (corresponding to 120 g of the load) at a weight-to-yarn mass ratio of 28 400, which was about 30 times more than the shape recovery stress of shape memory polymer. In terms of the stability study, the process of annealing and contraction training was introduced. In addition, the quantitative relationship between temperature and contraction was also rigorously explored, which facilitated a more accurate and controllable contractile stroke. Great potential applications ranging from soft robots, wearable intelligent devices, and biomimetic devices to self-deployable structures in the aerospace field are likely to benefit from the advantages of low density, fast response without hysteresis, super flexible structure, as well as stitchability and large-scale production.

18.
Exp Ther Med ; 15(1): 115-126, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29387185

RESUMO

Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized to inhibit the response of type I interferon (IFN) both in vivo and in vitro. However, the post-transcriptional mechanism by which PRRSV suppresses type I IFN induction in virus-infected host cells remains unclear. The present study first demonstrated that PRRSV inhibited post-transcriptionally the protein induction of IFN-ß in primary porcine alveolar macrophages (PAMs) during early infection, and the inhibition effect mediated by the Chinese highly pathogenic (HP)-PRRSV was stronger. Next, we analyzed the cellular microRNA (miRNA)-modulated protein expression of porcine IFN-ß by dual firefly/Renilla luciferase reporter assay, transfection of miRNA mimics and inhibitor assay and polyinosinic-polycytidylic acid (poly I:C) treatment of PAMs, showing that porcine miRNAs including let-7b, miR-26a, miR-34a and miR-145 are able to inhibit IFN-ß protein expression in primary PAMs by directly targeting sequences within the porcine IFN-ß 3'UTR locating at 160-181, 9-31, 27-47 and 12-32 bp, respectively. Finally, we confirmed that let-7b, miR-26a, miR-34a and miR-145, were upregulated in PRRSV-infected PAMs early in vitro, and the expression level of these miRNAs in HP-PRRSV JXwn06-infected PAMs were higher than those in low pathogenic PRRSV HB-1/3.9-infected PAMs. The endogenous cellular miRNA-mediated inhibition of IFN-ß induction in PRRSV-infected PAMs early could be relieved by miRNA antagonists. Taken together, our findings suggest for the first time that PRRSV can suppress post-transcriptionally protein expression of IFN-ß by upregulating cellular miRNAs in PAMs in vitro, providing novel insight into mechanisms in relation to the PRRSV-mediated immunomodulation of porcine innate immunity.

19.
J Environ Sci (China) ; 59: 30-38, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28888236

RESUMO

A heavy 16-day pollution episode occurred in Beijing from December 19, 2015 to January 3, 2016. The mean daily AQI and PM2.5 were 240.44 and 203.6µg/m3. We analyzed the spatiotemporal characteristics of air pollutants, meteorology and road space speed during this period, then extended to reveal the combined effects of traffic restrictions and meteorology on urban air quality with observational data and a multivariate mutual information model. Results of spatiotemporal analysis showed that five pollution stages were identified with remarkable variation patterns based on evolution of PM2.5 concentration and weather conditions. Southern sites (DX, YDM and DS) experienced heavier pollution than northern ones (DL, CP and WL). Stage P2 exhibited combined functions of meteorology and traffic restrictions which were delayed peak-clipping effects on PM2.5. Mutual information values of Air quality-Traffic-Meteorology (ATM-MI) revealed that additive functions of traffic restrictions, suitable relative humidity and temperature were more effective on the removal of fine particles and CO than NO2.


Assuntos
Poluentes Atmosféricos/análise , Poluição do Ar/estatística & dados numéricos , Monitoramento Ambiental , Pequim , Cidades/estatística & dados numéricos , Emissões de Veículos/análise
20.
Int J Biol Macromol ; 105(Pt 1): 764-768, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28732733

RESUMO

The purpose of this study was to understand the influence of Maillard reaction (MR) on bioactivity of chitosan oligosaccharide (COS). The antibacterial activity against Pseudomonas aeruginosa and Vibrio parahaemolyticus and preservative effect of two kinds of COS MR products (MRPs) on Penaeus vannamei were evaluated. The structures of CG (MRPs of COS and glucose) and CM (MRPs of COS and maltose) were characterized by FT-IR and their molecular weights were measured by gel permeation chromatography (GPC). The results showed that CG has enhanced antibacterial activity and preservative effect compared to COS by evaluating total volatile base-nitrogen (TVB-N), trimethylamine (TMA), pH, total bacterial count (TBC) and sensory evaluation. Meanwhile, CM showed decreased antibacterial activity and preservative effect compared to COS by evaluating TMA, pH and TBC. The results indicated that antibacterial activity and preservative effect of COS increased or decreased after MR. In other words, the type of reducing sugar involved in MR had a great impact on the functional properties of COS-saccharide MRPs and their application to be used as a food preservative.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Quitosana/química , Reação de Maillard , Oligossacarídeos/química , Oligossacarídeos/farmacologia , Penaeidae/efeitos dos fármacos , Animais , Conservação de Alimentos , Glucose/química , Concentração de Íons de Hidrogênio , Maltose/química , Peso Molecular , Paladar/efeitos dos fármacos
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