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1.
J Chem Inf Model ; 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34619030

RESUMO

Accurate modeling of compound bioactivities is essential for the virtual screening of drug leads. In real-world scenarios, pharmacists tend to choose from the top-k hit compounds ranked by predicted bioactivities from a large database with interest to continue wet experiments for drug discovery. Significant improvement of the precision of the top hits in ligand-based virtual screening of drug leads is more valuable than conventional schemes for accurately predicting the bioactivities of all compounds from a large database. Here, we proposed a new method, RealVS, to significantly improve the top hits' precision and learn interpretable key substructures associated with compound bioactivities. The features of RealVS involve the following points. (1) Abundant transferable information from the source domain was introduced for alleviating the insufficiency of inactive ligands associated with drug targets. (2) The adversarial domain alignment was adopted to fit the distribution of generated features of compounds from the training data set and that from the screening database for greater model generalization ability. (3) A novel objective function was proposed to simultaneously optimize the classification loss, regression loss, and adversarial loss, where most inactive ligands tend to be screened out before activity regression prediction. (4) Graph attention networks were adopted for learning key substructures associated with ligand bioactivities for better model interpretability. The results on a large number of benchmark data sets show that our method has significantly improved the precision of top hits under various k values in ligand-based virtual screening of drug leads from large compound databases, which is of great value in real-world scenarios. The web server of RealVS is freely available at noveldelta.com/RealVS for academic purposes, where virtual screening of hits from large compound databases is accessible.

2.
IEEE Trans Cybern ; PP2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34464284

RESUMO

In this article, being different from conventional time-discretization (simply called discretization) formulas, explicit linear left-and-right 5-step (ELLR5S) formulas with sixth-order precision are proposed. The general sixth-order ELLR5S formula with four variable parameters is developed first, and constraints of these four parameters are displayed to guarantee the zero stability, consistence, and convergence of the formula. Then, by choosing specific parameter values within constraints, eight specific sixth-order ELLR5S formulas are developed. The general sixth-order ELLR5S formula is further utilized to generate discrete zeroing neural network (DZNN) models for solving time-varying linear and nonlinear systems. For comparison, three conventional discretization formulas are also utilized. Theoretical analyses are presented to show the performance of ELLR5S formulas and DZNN models. Furthermore, abundant experiments, including three practical applications, that is, angle-of-arrival (AoA) localization and two redundant manipulators (PUMA560 manipulator and Kinova manipulator) control, are conducted. The synthesized results substantiate the efficacy and superiority of sixth-order ELLR5S formulas as well as the corresponding DZNN models.

3.
J Wound Care ; 30(8): 594-597, 2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34382848

RESUMO

Given the current COVID-19 crisis, multiple clinical manifestations and related complications of COVID-19 disease, especially in lung transplant patients following post-COVID-19 pneumonia, are a major challenge. Herein, we report the therapeutic course of the first reported case of sacrococcyx pressure ulcers (PU) in a 65-year-old male COVID-19 patient who underwent lung transplantation and developed a PU following surgery. We used a combination of regulated negative pressure-assisted wound therapy system (RNPT, six treatment courses, five days per treatment course), a skin tension-relief system (an intraoperative aid in minimising wounds caused by sacrococcygeal PUs) and a gluteus maximus myocutaneous flap to repair sacrococcygeal wounds. This successfully treated case provides a reference point for the treatment of similar cases.


Assuntos
COVID-19 , Transplante de Pulmão , Lesão por Pressão , Idoso , Humanos , Masculino , SARS-CoV-2 , Retalhos Cirúrgicos
4.
Sci Rep ; 11(1): 13764, 2021 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-34215767

RESUMO

With the development of artificial intelligence, technique improvement of the classification of skin disease is addressed. However, few study concerned on the current classification system of International Classification of Diseases, Tenth Revision (ICD)-10 on Diseases of the skin and subcutaneous tissue, which is now globally used for classification of skin disease. This study was aimed to develop a new taxonomy of skin disease based on cytology and pathology, and test its predictive effect on skin disease compared to ICD-10. A new taxonomy (Taxonomy 2) containing 6 levels (Project 2-4) was developed based on skin cytology and pathology, and represents individual diseases arranged in a tree structure with three root nodes representing: (1) Keratinogenic diseases, (2) Melanogenic diseases, and (3) Diseases related to non-keratinocytes and non-melanocytes. The predictive effects of the new taxonomy including accuracy, precision, recall, F1, and Kappa were compared with those of ICD-10 on Diseases of the skin and subcutaneous tissue (Taxonomy 1, Project 1) by Deep Residual Learning method. For each project, 2/3 of the images were included as training group, and the rest 1/3 of the images acted as test group according to the category (class) as the stratification variable. Both train and test groups in the Projects (2 and 3) from Taxonomy 2 had higher F1 and Kappa scores without statistical significance on the prediction of skin disease than the corresponding groups in the Project 1 from Taxonomy 1, however both train and test groups in Project 4 had a statistically significantly higher F1-score than the corresponding groups in Project 1 (P = 0.025 and 0.005, respectively). The results showed that the new taxonomy developed based on cytology and pathology has an overall better performance on predictive effect of skin disease than the ICD-10 on Diseases of the skin and subcutaneous tissue. The level 5 (Project 4) of Taxonomy 2 is better on extension to unknown data of diagnosis system assisted by AI compared to current used classification system from ICD-10, and may have the potential application value in clinic of dermatology.

5.
Commun Biol ; 4(1): 652, 2021 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-34079056

RESUMO

Hantaan viruses (HTNVs) are zoonotic pathogens transmitted mainly by rodents and capable of infecting humans. Increasing knowledge of the human response to HTNV infection can guide the development of new preventative vaccines and therapeutic strategies. Here, we show that HTNV can infect CD8+ T cells in vivo in patients diagnosed with hemorrhagic fever with renal syndrome (HFRS). Electron microscopy-mediated tracking of the life cycle and ultrastructure of HTNV-infected CD8+ T cells in vitro showed an association between notable increases in cytoplasmic multivesicular bodies and virus production. Notably, based on a clinical cohort of 280 patients, we found that circulating HTNV-infected CD8+ T cell numbers in blood were proportional to disease severity. These results demonstrate that viral infected CD8+ T cells may be used as an adjunct marker for monitoring HFRS disease progression and that modulating T cell functions may be explored for new treatment strategies.


Assuntos
Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/virologia , Vírus Hantaan/imunologia , Vírus Hantaan/patogenicidade , Febre Hemorrágica com Síndrome Renal/imunologia , Febre Hemorrágica com Síndrome Renal/virologia , Doença Aguda , Adulto , Linfócitos T CD8-Positivos/ultraestrutura , Micropartículas Derivadas de Células/ultraestrutura , Micropartículas Derivadas de Células/virologia , Citocinas/sangue , Progressão da Doença , Feminino , Vírus Hantaan/fisiologia , Febre Hemorrágica com Síndrome Renal/sangue , Humanos , Técnicas In Vitro , Masculino , Microscopia Eletrônica de Transmissão , Pessoa de Meia-Idade , Modelos Biológicos , Vírion/imunologia , Vírion/patogenicidade , Replicação Viral
6.
J BUON ; 26(2): 418-428, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34076988

RESUMO

PURPOSE: To quantify the expression of miR-497 and its target gene VEGF-B in patients with hepatocellular carcinoma (HCC), and microvascular invasion (MVI) to identify their relationship with clinicopathological characteristics and prognosis. METHODS: Imaging data of postoperative cancer and adjacent tissues of HCC patients with MVI diagnosed by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) were retrospectively analyzed. The expression of miR-497 in clinical samples and HepG2 and SMMC-7721 cell lines was quantified by quantitative PCR (Q-PCR). Correlations between miR-497 and patient survival and VEGF-B were explored in TCGA database. The invasion and migration of SMMC-7721 cells were tested by transwell assay. The binding sites between miR-497 and its target gene VEGF-B were verified by dual-luciferase reporter (DLR) assay, and VEGF-B levels were analyzed by western blot (WB). RESULTS: miR-497 showed a lower expression in HCC patients with MVI than those without MVI. It was also lowly expressed in HCC cell lines compared to normal liver cell lines. The proliferation and migration in HCC cells were inhibited by overexpression of miR-497, which were enhanced after transfection with miR-497 inhibitor. miR-497 had an effect on VEGF-B levels and there was a regulatory relationship between them. miR-497 was able to target VEGF-B and downregulate the receptor of VEGF-B (FLT-1). CONCLUSION: miR-497 was lowly expressed in HCC tissues, and its overexpression inhibited invasion and metastasis in HCC cells by suppressing VEGF-B levels. MiR-497 and its target gene VEGF-B are closely associated with the biological function and may serve as prognostic factors of MVI in patients with HCC.

7.
BMC Infect Dis ; 21(1): 445, 2021 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-34001041

RESUMO

BACKGROUND: Hemorrhagic fever with renal syndrome (HFRS) caused by Hantaan virus is characterized by systemic immunopathological injury. Pentraxin-3 is an acute-phase reactant involved in the processes of inflammation and infection. This study aimed to investigate the levels of plasma pentraxin-3 and evaluate its predictive value on disease severity and mortality risk in patients with HFRS. METHODS: This was a prospective real-world observational study. The concentrations of plasma pentraxin-3 were measured by enzyme linked immunosorbent assay (ELISA) in 105 HFRS patients and 27 healthy controls. We analyzed the clinical relevance between pentraxin-3 and clinical subtyping, hospital stay and conventional laboratory parameters of HFRS patients. Considering the prognosis (death) as the primary endpoint, the levels of pentraxin-3 between survivors and non-survivors were compared, and its association with mortality was assessed by Kaplan-Meier survival analysis. The predictive potency of pentraxin-3 for mortality risk in HFRS patients was evaluated by receiver operating characteristic (ROC) curve analysis. RESULTS: The levels of pentraxin-3 during the acute phase were increased with the aggravation of the disease, and showed the highest expression in critical-type patients (P < 0.05). Pentraxin-3 demonstrated significant correlations with conventional laboratory parameters (WBC, PLT, AST, ALB, APTT, Fib) and the length of hospital stay. Compared with the survivors, non-survivors showed higher levels of pentraxin-3 and worse expressions of conventional laboratory parameters during the acute phase. The Kaplan-Meier survival curves showed that high levels of pentraxin-3 during the acute phase were significantly associated with the death in HFRS patients. Pentraxin-3 demonstrated significant predictive value for the mortality risk of HFRS patients, with the area under ROC curve (AUC) of 0.753 (95%CI: 0.593 ~ 0.914, P = 0.003). CONCLUSIONS: The detection of plasma pentraxin-3 might be beneficial to the evaluation of disease severity and to the prediction of mortality risk in HFRS patients.


Assuntos
Proteína C-Reativa/análise , Febre Hemorrágica com Síndrome Renal/patologia , Componente Amiloide P Sérico/análise , Doença Aguda , Adulto , Área Sob a Curva , Feminino , Vírus Hantaan/isolamento & purificação , Febre Hemorrágica com Síndrome Renal/mortalidade , Febre Hemorrágica com Síndrome Renal/virologia , Humanos , Estimativa de Kaplan-Meier , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Prognóstico , Estudos Prospectivos , Curva ROC , Índice de Gravidade de Doença
8.
Surg Today ; 2021 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-33956234

RESUMO

PURPOSES: We introduce a novel 2-cm single port designed to minimize intercostal muscle and nerve damage in video-assisted thoracoscopic surgery (VATS) lobectomy, and compared it with the 3-cm traditional single port. METHODS: We analyzed, retrospectively, the clinical data, safety, convenience, incision complications, and postoperative pain and numbness in 81 patients who underwent either modified (n = 42) or traditional (n = 39) single-port VATS lobectomy. RESULTS: The preoperative variables were comparable between both single-port VATS lobectomy groups after matching. There were no serious complications and there was no mortality in either group. There were no remarkable differences between the groups in intraoperative blood loss, chest tube duration, lymph node dissection, or postoperative complications. The modified single-port group had a longer operation time (p < 0.05), but the static and dynamic postoperative VAS scores and incisional numbness were better in the modified single-port group (p < 0.05). The modified single-port group also had an obvious advantage in incision seepage, healing, and appearance. CONCLUSIONS: Our 2-cm modified single port for lobectomy is safe and effective, and results in less postoperative pain and incisional numbness than the 3-cm traditional single port.

9.
Urol J ; 18(3): 247-251, 2021 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-33840084

RESUMO

PURPOSE: To explore the efficacy of 5-ARIs in PCA (Prostate Cancer). METHODS: Searching through the major medical databases such as PubMed, Science Citation Index, EMBASE, Medline, Web of Science, Cochrane Library for all published studies in English until 2018. The following search terms were used: "Finasteride", "dutasteride", "5α reductase inhibitors", "5-ARIs", "prostate cancer", "prostate neoplasm" and the additional related studies were manually searched. Newcastle-Ottawa Scale (NOS) assessed the qualities of studies, and the outcome measures were observed by RR or OR with 95% CIs. RESULTS: We included 9 eligible studies for analyses from 2011 to 2017. We found that 5-ARIs group may have fewer progression (OR = 0.48 95%CI: 0.37-0.61 P < 0.00001, I2=4% p = 0.39) and lower pathological progression (OR = 0.46; 95%CI: 0.29-0.73; p = 0.001, I2=0% p = 0.45), compared with control groups. However, the OS did not show significant difference between two groups (OR=1.10; 95%CI:0.90-1.35; P = 0.35, I2 = 93% P < .00001 ). CONCLUSION: The use of 5-ARIs could prevent progression in PCA patients both clinical and pathological.

10.
Scott Med J ; 66(3): 108-114, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33663273

RESUMO

AIMS: To search for biochemical indicators that can identify symptomatic patients with COVID-19 whose nucleic acid could turn negative within 14 days, and assess the prognostic value of these biochemical indicators in patients with COVID-19. PATIENTS AND METHODS: We collected the clinical data of patients with COVID-19 admitted to our hospital, by using logistic regression analysis and AUC curves, explored the relationship between biochemical indicators and nucleic acid positive duration, the severity of COVID-19, and hospital stay respectively. RESULTS: A total of two hundred and thirty-three patients with COVID-19 were enrolled in the study. We found patients whose nucleic acid turned negative within 14 days had lower LDH, CRP and higher ALB (P < 0.05). ROC curve results indicated that lower LDH, TP, CRP and higher ALB predicted the nucleic acid of patients turned negative within 14 days with statistical significance(P < 0.05), AST, LDH, CRP and PCT predicted the severe COVID-19 with statistical significance, and CRP predicted hospital stay >31days with statistical significance (P < 0.05). After verification, the probability of nucleic acid turning negative within 14 days in patients with low LDH (<256 U/L), CRP (<44.5 mg/L) and high ALB (>35.8 g/L) was about 4 times higher than that in patients with high LDH, CRP and low ALB (P < 0.05). CONCLUSIONS: LDH, CRP and ALB are useful prognostic marker for predicting nucleic acid turn negative within 14 days in symptomatic patients with COVID-19.


Assuntos
Proteína C-Reativa/análise , COVID-19/sangue , DNA Viral/sangue , L-Lactato Desidrogenase/sangue , SARS-CoV-2/genética , Albumina Sérica/análise , Biomarcadores/sangue , COVID-19/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Prognóstico , Curva ROC , Estudos Retrospectivos , Fatores de Tempo
11.
Chemistry ; 27(26): 7444-7452, 2021 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-33686735

RESUMO

A crucial parameter in the design of semiconductor nanoparticles (NPs) with controllable optical, magnetic, electronic, and catalytic properties is the morphology. Herein, we demonstrate the potential of additive metal cations with variable valency to direct the morphology evolution of copper-deficient Cu2-x S nanoparticles in the process of seed-mediated growth. In particular, the djurleite Cu1.94 S seed could evolve from disk into tetradecahedron in the presence of tin(IV) cations, whereas they merely formed sharp hexagonal nanodisks with tin(II) cations. In addition to djurleite Cu1.94 S, the tin(IV) cations could be generalized to direct the growth of roxbyite Cu1.8 S and covellite CuS nanodisk seeds into tetradecahedra. We further perform pseudomorphic cation exchanges of Cu1.94 S tetradecahedra with Zn2+ and Cd2+ to produce polyhedral zinc sulfide (ZnS) and cadmium sulfide (CdS) NPs. Moreover, we achieve Cu1.8 S/ZnS and Cu1.94 S/CdS tetradecahedral heterostructures via partial cation exchange, which are otherwise inaccessible by traditional synthetic approaches.

12.
Pers Ubiquitous Comput ; : 1-13, 2021 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-33679281

RESUMO

This paper describes a novel approach to measure individual investor sentiment using text-based analysis of millions of posts extracted from Chinese financial online forums. We describe how we built a database of more than 200 million stock posts from online financial forums, created GubaLex, a sentiment dictionary consisting of 48,878 words to allow sentiment analysis, and how we developed GubaSenti, an individual investor sentiment index for the stock market in China. This allowed (1) the first systemic measurement of individual investor sentiment in China; (2) an approach to text-based analysis that reflects investor sentiment about millions of posts about stocks listed in Guba; (3) a way to flexibly measure investor sentiment of a single stock, a sector or an industry and the whole market; and (4) made this possible for daily, weekly, monthly, quarterly, and yearly time periods. We also examine the relationship of the sentiment proxy and stock returns and compare it with two typical BW metrics in China. Empirical results show that GubaSenti correlates better with market performance than BW metrics in China and can be used to predict market changes in the short term.

13.
Cell Death Dis ; 12(2): 156, 2021 02 04.
Artigo em Inglês | MEDLINE | ID: mdl-33542183

RESUMO

Mesenchymal stem cells (MSCs) have the potential to reduce healing time and treat nonunion in fracture patients. In this study, bone marrow MSCs-derived extracellular vesicles (B-EVs) were firstly extracted and identified. CD9-/- and normal mice were enrolled for the establishment of fracture models and then injected with B-EVs. Osteoblast differentiation and fracture recovery were estimated. The levels of osteoblast-related genes were detected, and differentially expressed microRNAs (miRs) in B-EVs-treated normal fracture mice were screened and verified. The downstream mechanisms of miR were predicted and assessed. The loss-of functions of miR-335 in B-EV and gain-of-functions of VapB were performed in animal and cell experiments to evaluate their roles in bone fracture. Collectively, B-EVs promoted bone fracture recovery and osteoblast differentiation by releasing miR-335. miR-335 downregulation in B-EVs impaired B-EV functions in fracture recovery and osteoblast differentiation. miR-335 could target VapB, and VapB overexpression reversed the effects of B-EVs on osteoblast differentiation. B-EV treatment activated the Wnt/ß-catenin pathway in fracture mice and osteoblasts-like cells. Taken together, the study suggested that B-EVs carry miR-335 to promote bone fracture recovery via VapB and the Wnt/ß-catenin pathway. This study may offer insights into bone fracture treatment.


Assuntos
Exossomos/metabolismo , Exossomos/transplante , Fraturas do Fêmur/cirurgia , Fêmur/metabolismo , Consolidação da Fratura , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , MicroRNAs/metabolismo , Osteoblastos/metabolismo , Células 3T3 , Animais , Diferenciação Celular , Modelos Animais de Doenças , Exossomos/genética , Fraturas do Fêmur/genética , Fraturas do Fêmur/metabolismo , Fraturas do Fêmur/patologia , Fêmur/lesões , Fêmur/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , MicroRNAs/genética , Osteoblastos/patologia , Osteogênese , Tetraspanina 29/genética , Tetraspanina 29/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Via de Sinalização Wnt
14.
Acta Pharmacol Sin ; 2021 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33574569

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers, with a minimal difference between its incidence rate and mortality rate. Advances in oncology over the past several decades have dramatically improved the overall survival of patients with multiple cancers due to the implementation of new techniques in early diagnosis, therapeutic drugs, and personalized therapy. However, pancreatic cancers remain recalcitrant, with a 5-year relative survival rate of <9%. The lack of measures for early diagnosis, strong resistance to chemotherapy, ineffective adjuvant chemotherapy and the unavailability of molecularly targeted therapy are responsible for the high mortality rate of this notorious disease. Genetically, PDAC progresses as a complex result of the activation of oncogenes and inactivation of tumor suppressors. Although next-generation sequencing has identified numerous new genetic alterations, their clinical implications remain unknown. Classically, oncogenic mutations in genes such as KRAS and loss-of-function mutations in tumor suppressors, such as TP53, CDNK2A, DPC4/SMAD4, and BRCA2, are frequently observed in PDAC. Currently, research on these key driver genes is still the main focus. Therefore, studies assessing the functions of these genes and their potential clinical implications are of paramount importance. In this review, we summarize the biological function of key driver genes and pharmaceutical targets in PDAC. In addition, we conclude the results of molecularly targeted therapies in clinical trials and discuss how to utilize these genetic alterations in further clinical practice.

15.
IEEE Trans Cybern ; PP2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33544686

RESUMO

Differing from the common linear matrix equation, the future different-level linear matrix system is considered, which is much more interesting and challenging. Because of its complicated structure and future-computation characteristic, traditional methods for static and same-level systems may not be effective on this occasion. For solving this difficult future different-level linear matrix system, the continuous different-level linear matrix system is first considered. On the basis of the zeroing neural network (ZNN), the physical mathematical equivalency is thus proposed, which is called ZNN equivalency (ZE), and it is compared with the traditional concept of mathematical equivalence. Then, on the basis of ZE, the continuous-time synthesis (CTS) model is further developed. To satisfy the future-computation requirement of the future different-level linear matrix system, the 7-instant discrete-time synthesis (DTS) model is further attained by utilizing the high-precision 7-instant Zhang et al. discretization (ZeaD) formula. For a comparison, three different DTS models using three conventional ZeaD formulas are also presented. Meanwhile, the efficacy of the 7-instant DTS model is testified by the theoretical analyses. Finally, experimental results verify the brilliant performance of the 7-instant DTS model in solving the future different-level linear matrix system.

16.
Molecules ; 26(2)2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33478072

RESUMO

The use of arginine deiminase (ADI) for arginine depletion therapy is an attractive anticancer approach. Combination strategies are needed to overcome the resistance of severe types of cancer cells to this monotherapy. In the current study, we report, for the first time, that the antioxidant N-acetylcysteine (NAC), which has been used in therapeutic practices for several decades, is a potent enhancer for targeted therapy that utilizes arginine deiminase. We demonstrated that pegylated arginine deiminase (ADI-PEG 20) induces apoptosis and G0/G1 phase arrest in murine MC38 colorectal cancer cells; ADI-PEG 20 induces Ca2+ overload and decreases the mitochondrial membrane potential in MC38 cells. ADI-PEG 20 induced the most important immunogenic cell death (ICD)-associated feature: cell surface exposure of calreticulin (CRT). The antioxidant NAC enhanced the antitumor activity of ADI-PEG 20 and strengthened its ICD-associated features including the secretion of high mobility group box 1 (HMGB1) and adenosine triphosphate (ATP). In addition, these regimens resulted in phagocytosis of treated MC38 cancer cells by bone marrow-derived dendritic cells (BMDCs). In conclusion, we describe, for the first time, that NAC in combination with ADI-PEG 20 not only possesses unique cytotoxic anticancer properties but also triggers the hallmarks of immunogenic cell death. Hence, ADI-PEG 20 in combination with NAC may represent a promising approach to treat ADI-sensitive tumors while preventing relapse and metastasis.


Assuntos
Acetilcisteína/química , Neoplasias da Mama/patologia , Neoplasias Colorretais/patologia , Hidrolases/química , Hidrolases/farmacologia , Morte Celular Imunogênica/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Humanos , Camundongos , Polietilenoglicóis/química
17.
Dis Esophagus ; 34(1)2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-32844223

RESUMO

It seems impossible to reconstruct the esophagus of patients with middle thoracic esophageal carcinoma with a history of distal gastrectomy using the remnant stomach. Although surgeons have made multiple efforts to reconstruct the esophagus using the remnant stomach, it can only be successfully used in cases of lower thoracic esophageal cancer. Additionally, the surgery is more complex than traditional esophagogastrostomy due to challenges including mobilization of the remnant stomach with the spleen and transposition of the pancreatic tail into the left hemithorax. Our operation proved that the remnant stomach, which we named as the completely mobilized remnant stomach after dissection of the feeding vessels, remained viable. We successfully integrated the completely mobilized remnant stomach in the reconstruction of the lower thoracic esophageal tract and then integrated it in Ivor Lewis esophagogastrostomy. We describe this new alternative surgical technique for the treatment of middle thoracic esophageal carcinoma in patients with a history of distal gastrectomy in this study. Clinical data of 23 patients from 2008 to 2019 were retrospectively analyzed. All patients underwent the Ivor Lewis procedure. All remaining vessels of the remnant stomach were dissected at their origins, and Roux-en-Y reconstruction or Braun anastomosis was performed. After esophagectomy during right thoracotomy, anastomosis of the remnant stomach and esophagus was performed. Two-field lymph node dissections were performed. There was no case of necrosis of the remnant stomach or of perioperative death. Serious complications included anastomotic leak in three cases, afferent-efferent loop syndrome in one, and anastomotic stricture in two. Application of the completely mobilized remnant stomach in Ivor Lewis esophagogastrostomy is feasible, and the surgical procedure is similar to that of normal esophagogastrostomy.

18.
Surg Today ; 51(1): 172-175, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32328737

RESUMO

Independent subsuperior segmentectomy (S*) via uniportal video-assisted thoracoscopic surgery (VATS) has rarely been reported. We describe our modified technique of performing simplified left subsuperior segmentectomy for a lung nodule, via 2-cm uniportal VATS. The uniportal approach was different from the traditional approach made by blunt separation into the thorax without electrocautery. Our modified technique minimizes damage to the intercostal nerves and muscles. We also simplified the subsuperior segmentectomy procedure according to the findings of three-dimensional (3D) computed tomography angiography and bronchography. Combining these two techniques achieves a new more minimally invasive method for subsuperior segmentectomy.


Assuntos
Broncografia/métodos , Angiografia por Tomografia Computadorizada/métodos , Imageamento Tridimensional/métodos , Pulmão/cirurgia , Pneumonectomia/métodos , Nódulo Pulmonar Solitário/cirurgia , Cirurgia Assistida por Computador/métodos , Cirurgia Torácica Vídeoassistida/métodos , Idoso , Feminino , Humanos , Doença Iatrogênica/prevenção & controle , Nervos Intercostais/lesões , Complicações Intraoperatórias/prevenção & controle , Traumatismos dos Nervos Periféricos/prevenção & controle
19.
Chemistry ; 27(3): 1057-1065, 2021 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-33051913

RESUMO

For plasmonic copper-deficient Cu2-x S nanoparticles (NPs), accurate control of the crystal phase and morphology is highly desirable as both of which are known to determine the localized surface plasmon resonance (LSPR) wavelength and amplitude. Here, how the sulfur precursor reactivity in the synthesis of Cu2-x S NPs affects the resulting crystal phase and morphology is examined. Djurleite Cu1.94 S, roxbyite Cu1.8 S, digenite Cu1.8 S as well as covellite CuS nanodisks were synthesized by using 1-dodecanethiol, N,N-dibutylthiourea, and crystal sulfur 1-octadecene/oleylamine solutions and their crystal phase dependent LSPR properties were exhaustively discussed. In addition, crystal phase interconversion between covellite CuS and djurleite/roxbyite Cu2-x S was realized in the presence of the above sulfur precursors. On the other hand, djurleite Cu1.94 S nanorods rather than nanodisks were prepared by replacing 1-dodecanethiol with more reactive tert-dodecanethiol. The structural and morphological Cu2-x S NPs here holds great promise in the application of photothermal therapy, photocatalysis, surface-enhanced Raman scattering (SERS), and many others.

20.
ACS Appl Mater Interfaces ; 12(50): 56039-56048, 2020 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-33263995

RESUMO

Crystal facet engineering provides a promising approach to tailor the performance of catalysts because of the close relationship between the photocatalytic activity and the surface atomic and electronic structures. An in-depth understanding mechanism of crystal facet-dependent CO2 photoreduction is still an open question. Herein, two different types of porous ZnO nanocatalysts are used as model photocatalysts for the investigation, which are, respectively, with exposed {110} and {001} facets. The porous ZnO with an exposed {110} facet exhibits superior photocatalytic activity to the one with the {001} facet. Various influencing factors have been thoroughly studied both theoretically and/or experimentally, including light harvesting (i.e., band gap), reduction capability (potential of conduction band), crystallinity, CO2 adsorption ability, CO2 activation, and charge separation. The major influencing factors are eventually figured out based on the experimental and calculation results. The product selectivity and the influence of the hole scavenger can be explained too. Our work may pave a way for directing the future rational design of efficient photocatalysts for CO2 reduction.

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