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1.
Environ Pollut ; 263(Pt A): 114293, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-32208227

RESUMO

Haze pollution is inseparable from the transformation of air pollutants especially the ammonium and sulfate. Chemical and physical processes play important roles in this transformation. However, the role of microbial processes has rarely been studied. In this report, we applied the cultivation-independent metagenomic approach to study airborne microorganisms, investigating the potential microbial-catalyzed transformation of ammonium and sulfate in PM2.5 samples. Functional genes predict that airborne microorganisms have the potential to catalyze ammonium formation but not ammonium oxidation since no ammoxidation genes were identified. We also found that the frequency of sulfate-forming genes was 1.56 times of those for sulfate-reducing genes. It was speculated that microbial metabolisms in the atmosphere could contribute to the accumulation of ammonium and sulfate. With the increase of PM2.5 concentration, the frequency of functional genes and the relative abundance of genera which involved in nitrogen and sulfur metabolisms increased. That suggested air pollution was conducive to the microbial-mediated formation of ammonium and sulfate. Overall, our results provided evidence for the possible role of microbial processes in the air pollutant transformation and brought a new perspective for studying the formation of secondary air pollutants.

2.
Colloids Surf B Biointerfaces ; 190: 110922, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32179415

RESUMO

Air pollution has become increasingly serious. Fine particulate matter (PM2.5) is the most well-known air pollutant, which leads to some common respiratory diseases when inhaled into the lungs to certain concentration. However, there is a lack of research on the process of dynamically monitoring the real-time effect of nanoparticles on the pulmonary surfactant monolayer. In this study, the DPPC/DPPG monolayer is prepared by the Langmuir method to simulate the lung surfactant monolayer during respiration and the carbon nanoparticles are introduced to the monolayer under different surface pressures to simulate the real dynamic process of inhaling nanoparticles during breathing. The effect of carbon nanoparticles on the surface behavior of DPPC/DPPG monolayer in real-time was examined in details by a combination of surface pressure (π)-area (A) isotherms, compressibility modulus (Cs-1)-surface pressure (π) isotherms and the Brewster angle microscopy (BAM). The results have shown that the introduction of carbon nanoparticles under different surface pressures affects the properties of lipid monolayers. The added carbon nanoparticles under lower surface pressure are easy to penetrate the lipid molecules to inhibit monolayer phase transition. When the carbon nanoparticles are introduced to the monolayer under higher surface pressure, they tend to self-aggregate to reduce the monolayer stability rather than interact with lipid tail chains. This work not only confirms the exotic hydrophobic carbon nanoparticles retain in the DPPC/DPPG monolayer irreversibly and affect the surface behavior of monolayer during respiration, but also opens a new idea for real-time monitoring of the effects of PM2.5 on lung health.

3.
J Hazard Mater ; 383: 121141, 2020 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-31610411

RESUMO

This work combined a Deep Neural Network (DNN) with molecular fingerprints (MF) to develop models to predict the OH radical rate constants of 593 organic contaminants. Molecular descriptors, most often used in establishing quantitative structural-activity relationships (QSARs), were not used here because of their complicated generation processes that rely on advanced physicochemical and computational knowledge. Instead, we only fed the most basic information of the contaminant structures, i.e., MF encoding the types of atoms and how they are connected, to DNN and DNN then developed predictive models automatically. Here, a dataset containing 457 contaminants and their OH rate constants was first used to develop predictive models by DNN-MF. The hence developed models showed comparable accuracy to the traditional QSARs. The root mean square error (RMSE) values of the test sets were 0.358-0.384. The length of 2048 bits for the MF and 3 hidden layers (each with 1024 neurons) were found to be the optimal parameters for DNN. The model containing additional 89 micorpollutants in the training set was then successfully applied to predict the OH rate constants of 17 organophosphorus flame retardants and 29 additional micropollutants, with comparable accuracy to the reported molecular descriptors-based QSARs.

4.
Sheng Wu Gong Cheng Xue Bao ; 35(11): 2189-2200, 2019 Nov 25.
Artigo em Chinês | MEDLINE | ID: mdl-31814364

RESUMO

Siderophore is a chelating iron substance secreted by microorganisms at low intracellular iron concentration. Siderophore can be divided into three categories: catechol salts, hydroxamic salts and carboxylates. The transport of siderophore is regulated by Fur, σ factor and quorum sensing signal. In recent years, siderophore has been used in fields such as oil pollution remediation, heavy metal pollution remediation and pulp biological bleaching, and has received extensive attention. This paper reviews the classification of siderophores and their transport regulation mechanism, and the application of siderophore in environmental pollution control and remediation. Furthermore, we address the application of siderophore in the future.


Assuntos
Recuperação e Remediação Ambiental , Sideróforos , Transporte Biológico , Sideróforos/classificação , Sideróforos/metabolismo
5.
Langmuir ; 35(41): 13452-13460, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31524404

RESUMO

Pulmonary administration is widely used for the treatment of lung diseases. The interaction between drug molecules and pulmonary surfactants affects the efficacy of the drug directly. The location and distribution of drug molecules in a model pulmonary surfactant monolayer under different surface pressures can provide vivid information on the interaction between drug molecules and pulmonary surfactants during the pulmonary administration. Ketoprofen is a nonsteroidal anti-inflammatory drug for pulmonary administration. The effect of ketoprofen molecules on the lipid monolayer containing 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-glycerol (DPPG) is studied by surface pressure (π)-area (A) isotherms and compressibility modulus (Cs-1)-surface pressure (π) isotherms. The location and distribution of ketoprofen molecules in a lipid monolayer under different surface pressures are explored by surface tension, density profile, radial distribution function (RDF), and the potential of mean force (PMF) simulated by molecular dynamics (MD) simulation. The introduction of ketoprofen molecules affects the properties of DPPC/DPPG monolayers and the location and distribution of ketoprofen molecules in monolayers with various surface pressures. The existence of ketoprofen molecules hinders the formation of liquid-condensed (LC) films and decreases the compressibility of DPPC/DPPG monolayers. The location and distribution of ketoprofen molecules in the lipid monolayer are affected by cation-π interaction between the choline group of lipids and the benzene ring of ketoprofen, the steric hindrance of the lipid head groups, and the hydrophobicity of ketoprofen molecule itself, comprehensively. The contact state of lipid head group with water is determined by surface pressure, which affects the interaction between drug molecules and lipids and further dominates the location and distribution of ketoprofen in the lipid monolayer. This work confirms that ketoprofen molecules can affect the property and the inner structure of DPPC/DPPG monolayers during breathing. Furthermore, the results obtained using a mixed monolayer containing two major pulmonary surfactants DPPC/DPPG and ketoprofen molecules will be helpful for the in-depth understanding of the mechanism of inhaled administration therapy.

6.
Mater Sci Eng C Mater Biol Appl ; 103: 109711, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31349489

RESUMO

Tendon calcification is a common but intractable problem leading to pain and activity limitation when injury or tendinopathy progresses into the late stage. This is because tendon stem/progenitor cells (TSPCs) can undergo aberrant osteogenic differentiation under inflammatory conditions. This study aims to investigate the effect of curcumin, a natural anti-inflammatory agent, on regulating the differentiation of TSPCs in tendon calcification. With inflammatory stimulation, TSPCs showed higher alkaline phosphatase activity and more frequent formation of mineralized nodules which were verified in the culture system; however, curcumin significantly alleviated these pathological changes. In in vivo function analysis, chitosan microsphere-encapsulated curcumin was delivered to injured sites of rat tendon ectopic calcification model. The inflammation in the tendon tissues of the curcumin group was significantly relieved. Controlled-release curcumin partially rescued tendon calcification and enhanced tendon regeneration in animal model. This study demonstrates that controlled-release curcumin can manipulate the fate decision of TSPCs, and that it promotes the tenogenesis and inhibits the osteogenesis of TSPCs in a pathological microenvironment, which provides a possible new therapeutic strategy for tendon disease.


Assuntos
Tendão do Calcâneo/metabolismo , Calcinose/tratamento farmacológico , Diferenciação Celular/efeitos dos fármacos , Curcumina/farmacologia , Células-Tronco/metabolismo , Tendão do Calcâneo/patologia , Animais , Calcinose/metabolismo , Calcinose/patologia , Curcumina/química , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacologia , Camundongos , Ratos , Células-Tronco/patologia
7.
Environ Pollut ; 247: 898-906, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30823344

RESUMO

Microorganisms are ubiquitous in the atmosphere, where they can disperse for a long distance. However, it remains poorly understood how these airborne microorganisms vary and which factors influence the microbial distribution in different anthropogenic activity regions. To explore the regional differences of bacteria and fungi in airborne particles, PM2.5 and PM10 samples were collected in the urban and rural areas of Hangzhou. The bacterial and fungal communities in the urban atmosphere was more similar to each other than those in the rural atmosphere. Analyses conducted by the concentration weighted trajectory model demonstrated that the local environment contributed more to the similarity of airborne bacteria and fungi compared with the atmospheric transport. The concentrations of local air pollutants (PM2.5, PM10, NO2, SO2 and CO) were positively correlated with the similarity of the bacterial and fungal communities. Additionally, the concentrations of these air pollutants in the urban site were about 1.5 times than those in the rural site. This implicated that anthropogenic activity, which is the essential cause of air pollutants, influenced the similarity of airborne bacteria and fungi in the urban area. This work ascertains the outdoor bacterial and fungal distribution in the urban and the rural atmosphere and provides a prospective model for studying the contributing factors of airborne bacteria and fungi.


Assuntos
Microbiologia do Ar , Poluentes Atmosféricos/análise , Bactérias/isolamento & purificação , Fungos/isolamento & purificação , Material Particulado/análise , China , Cidades , Monitoramento Ambiental
8.
J Gastrointest Surg ; 23(11): 2255-2262, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30859429

RESUMO

BACKGROUND: Intussusception is among the most common acute abdominal emergencies in infancy, but only some cases need surgical reduction. This study assessed the clinical characteristics of patients undergoing surgical reduction of intussusception. METHODS: This retrospective study reviewed 568 pediatric patients who failed air-enema reduction and underwent surgical reduction for intussusception in our department between 2008 and 2017. RESULTS: The series comprised 376 boys and 192 girls (2.0:1, male:female ratio) and most of the intussusceptions were primary, which is typical before the age of 1 year. The success rate of air-enema reduction in our hospital was 94.2%. Patients over 3 years old had the highest rate of surgical reduction (ca. 11.8%). The probabilities of primary and secondary intussusception were equal above 2 years old. Intussusception caused by intestinal malignant lymphoma was diagnosed above 2 years of age with atypical symptoms. Gender was irrelevant regarding the presence of bloody stools (P = 0.594), but the younger patients and children with complex/compound intussusception had a higher proportion of bloody stools (n = 148, 40.0%, P = 0.000) and intestinal necrosis (n = 44, 42.3%, P = 0.024). The occurrence time of bloody stools (OTBS) in complex/compound intussusception was shorter than for other types. CONCLUSIONS: This retrospective study analyzed the clinical features of patients undergoing surgical reduction for intussusception and summarized the characteristics and management of complex/compound intussusception.

9.
Microb Ecol ; 78(3): 555-564, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30903203

RESUMO

The intertidal zone is an important buffer and a nitrogen sink between land and sea. Ammonia oxidation is the rate-limiting step of nitrification, conducted by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, it remains a debatable issue regarding dominant ammonia oxidizers in this region, and environmental factors driving their spatiotemporal niche differentiation have yet to be identified. In this study, intertidal and subtidal zones of Zhoushan Islands were selected for seasonal sampling. Ammonia-oxidizing activity, quantitative PCR, and 454 high-throughput sequencing were performed to study the nitrification potential, abundance, and community structure of ammonia-oxidizing archaea and bacteria. AOA and AOB amoA abundance (107-108amoA gene copies/g dry weight sediment) varied spatiotemporally independently of environmental factors. AOA surpassed AOB in most samples, driven by sediment temperature, moisture, and total nitrogen. The diversity of both AOA and AOB differed spatiotemporally. The Nitrosopumilus and Nitrosospira clusters accounted for an absolutely dominant percentage of AOA (> 99%) and AOB (> 99%) respectively, indicating a negligible contribution of other clusters to ammonia oxidation. However, there was no significant correlation between nitrification potential and the abundance of AOA or AOB. Overall, the present study showed that AOA dominated over AOB spatiotemporally in the intertidal zone of Zhoushan Islands due to fluctuations in environmental factors, and the Nitrosopumilus and Nitrosospira clusters ecologically succeeded in the intertidal zone of Zhoushan Islands.


Assuntos
Archaea/isolamento & purificação , Betaproteobacteria/isolamento & purificação , Sedimentos Geológicos/microbiologia , Amônia/metabolismo , Archaea/classificação , Archaea/genética , Archaea/metabolismo , Betaproteobacteria/classificação , Betaproteobacteria/genética , Betaproteobacteria/metabolismo , China , Ecossistema , Ilhas , Nitrificação , Oxirredução , Filogenia
10.
3D Print Med ; 5(1): 5, 2019 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-30874929

RESUMO

BACKGROUND: Our long-term goal is to design and manufacture a customized graft with porous scaffold structure for repairing large mandibular defects using topological optimization and 3D printing technology. The purpose of this study is to characterize the mechanical behavior of 3D printed anisotropic scaffolds as bone analogs by fused deposition modeling (FDM). METHODS: Cone beam computed tomography (CBCT) images were used to reconstruct a 3D mandible and finite element models. A virtual sectioned-block of the mandible was used as the control group and the trabecular portion of the block was modified by topological optimization methods as experimental groups. FDM (FDM) printed samples at 0, 45 and 90 degrees with Poly-lactic acid (PLA) material under a three-point bending test. Finite element analysis was also used to validate the data obtained from the physical model tests. RESULTS: The ultimate load, yield load, failure deflection, yield deflection, stress, strain distribution, and porosity of scaffold structures were compared. The results show that the topological optimized graft had the best mechanical properties. CONCLUSIONS: The results from mechanical tests on physical models and numerical simulations from this study show a great potential for topological optimization and 3D printing technology to be served in design and rapidly manufacturing of artificial porous grafts.

11.
Appl Environ Microbiol ; 85(7)2019 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-30709818

RESUMO

Methane is a primary greenhouse gas which is responsible for global warming. The sulfate-dependent anaerobic methane oxidation (S-AOM) process catalyzed by anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB) is a vital link connecting the global carbon and sulfur cycles, and it is considered to be the overriding methane sink in marine ecosystem. However, there have been few studies regarding the role of S-AOM process and the distribution of ANME archaea in intertidal ecosystem. The intertidal zone is a buffer zone between sea and land and plays an important role in global geochemical cycle. In the present study, the abundance, potential methane oxidation rate, and community structure of ANME archaea in the intertidal zone were studied by quantitative PCR, stable isotope tracing method and high-throughput sequencing. The results showed that the potential S-AOM activity ranged from 0 to 0.77 nmol 13CO2 g-1 (dry sediment) day-1 The copy number of 16S rRNA gene of ANME archaea reached 106 ∼ 107 copies g-1 (dry sediment). The average contribution of S-AOM to total anaerobic methane oxidation was up to 34.5%, while denitrifying anaerobic methane oxidation accounted for the rest, which implied that S-AOM process was an essential methane sink that cannot be overlooked in intertidal ecosystem. The simulated column experiments also indicated that ANME archaea were sensitive to oxygen and preferred anaerobic environmental conditions. This study will help us gain a better understanding of the global carbon-sulfur cycle and greenhouse gas emission reduction and introduce a new perspective into the enrichment of ANME archaea.IMPORTANCE The sulfate-dependent anaerobic methane oxidation (S-AOM) process catalyzed by anaerobic methanotrophic (ANME) archaea and sulfate-reducing bacteria (SRB) is a vital link connecting the global carbon and sulfur cycles. We conducted a research into the spatial-temporal pattern of S-AOM process and the distribution of ANME archaea in coastal sediments collected from the intertidal zone. The results implied that S-AOM process was a methane sink that cannot be overlooked in the intertidal ecosystem. We also found that ANME archaea were sensitive to oxygen and preferred anaerobic environmental conditions. This study will help us gain a better understanding of the global carbon-sulfur cycle and greenhouse gas emission reduction and introduce a new perspective into the enrichment of ANME archaea.

12.
Environ Pollut ; 244: 228-237, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30342364

RESUMO

Coastal wetlands are widely recognized as atmospheric methane sources. However, recent field studies suggest that some coastal wetlands could also act as methane sinks, but the mechanism is not yet clear. Here, we investigated methane oxidation with different electron acceptors (i.e., oxygen, nitrate/nitrite, sulfate, Fe(III) and Mn(IV)) in four coastal wetlands in China using a combination of molecular biology methods and isotopic tracing technologies. The geochemical profiles and in situ Gibbs free energies suggest that there was significant nitrite-dependent anaerobic oxidation of methane (nitrite-AOM) in the sub-surface sediments; this was subsequently experimentally verified by both the microbial abundance and activity. Remarkably, the methanotrophic communities seemed to exist in the sediments as layered structures, and the surface aerobic methane-oxidizing bacteria were able to take up atmospheric methane at a rate of 0.10-0.18 nmol CH4 day-1 cm-2, while most, if not all, sedimentary methane was being completely consumed by anaerobic methanotrophs (23-58% by methane oxidizers in phylum NC10). These results suggest that coastal methane sinks might be governed by diverse microbial communities where NC10 methane oxidizers contributed significantly. This finding helps to better understand and predict the coastal methane cycle and reduce uncertainties in the estimations of the global methane flux.


Assuntos
Metano/química , Oxirredução , Anaerobiose , China , Compostos Férricos/química , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiologia , Manganês/química , Nitratos/química , Nitritos/química , Filogenia , Sulfatos/química , Áreas Alagadas
13.
Development ; 145(18)2018 09 20.
Artigo em Inglês | MEDLINE | ID: mdl-30111653

RESUMO

The differentiated phenotype of articular chondrocytes of synovial joints needs to be maintained throughout life. Disruption of the articular cartilage, frequently associated with chondrocyte hypertrophy and calcification, is a central feature in osteoarthritis (OA). However, the molecular mechanisms whereby phenotypes of articular chondrocytes are maintained and pathological calcification is inhibited remain poorly understood. Recently, the ecto-enzyme Enpp1, a suppressor of pathological calcification, was reported to be decreased in joint cartilage with OA in both human and mouse, and Enpp1 deficiency causes joint calcification. Here, we found that hedgehog (Hh) signaling activation contributes to ectopic joint calcification in the Enpp1-/- mice. In the Enpp1-/- joints, Hh signaling was upregulated. Further activation of Hh signaling by removing the patched 1 gene in the Enpp1-/- mice enhanced ectopic joint calcification, whereas removing Gli2 partially rescued the ectopic calcification phenotype. In addition, reduction of Gαs in the Enpp1-/- mice enhanced joint calcification, suggesting that Enpp1 inhibits Hh signaling and chondrocyte hypertrophy by activating Gαs-PKA signaling. Our findings provide new insights into the mechanisms underlying Enpp1 regulation of joint integrity.


Assuntos
Calcinose/patologia , Condrócitos/patologia , Proteínas Hedgehog/metabolismo , Artropatias/patologia , Articulações/patologia , Osteoartrite/patologia , Diester Fosfórico Hidrolases/genética , Pirofosfatases/genética , Doenças Vasculares/patologia , Animais , Diferenciação Celular/genética , Condrócitos/citologia , Cromograninas/genética , Cromograninas/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Feminino , Subunidades alfa Gs de Proteínas de Ligação ao GTP/genética , Subunidades alfa Gs de Proteínas de Ligação ao GTP/metabolismo , Proteínas Hedgehog/antagonistas & inibidores , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptor Patched-1/genética , Transdução de Sinais , Membrana Sinovial/citologia , Proteína Gli2 com Dedos de Zinco/genética
14.
Bone ; 109: 134-142, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-28855144

RESUMO

Bone formation is exquisitely controlled both spatially and temporally. Heterotopic ossification (HO) is pathological bone formation in soft tissues that often leads to deleterious outcomes. Inherited genetic forms of HO can be life-threatening and can happen as early as in infancy. However, there is currently no effective treatment for HO as the underlying cellular and molecular mechanisms have not been completely elucidated. Trauma-induced non-genetic forms of HO often occur as a common complication after surgeries or accidents, and the location of HO occurrence largely determines the symptom and outcome. While it has been difficult to determine the complicated factors causing HO, recent advancement in identifying cellular and molecular mechanism causing the genetic forms of HO may provide important insights in all HO. Here in this review, we summarize recent studies on HO to provide a current status of both clinical options of HO treatments and mechanical understanding of HO.


Assuntos
Doenças Ósseas Metabólicas/metabolismo , Miosite Ossificante/metabolismo , Ossificação Heterotópica/metabolismo , Dermatopatias Genéticas/metabolismo , Animais , Doenças Ósseas Metabólicas/patologia , Humanos , Miosite Ossificante/patologia , Ossificação Heterotópica/patologia , Dermatopatias Genéticas/patologia
15.
Onco Targets Ther ; 10: 5727-5738, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29238205

RESUMO

Objective: The objective of the study was to evaluate the prognostic value of positron emission tomography (PET)/computed tomography (CT) visual interpretation in patients with aggressive non-Hodgkin's lymphoma (NHL) using a meta-analysis and systematic review. Methods: Using the PubMed, Embase, and Web of Science databases, we performed a systematic review of the use of visual evaluation mid-chemotherapy to evaluate the prognosis of aggressive NHL in studies published up to May 2017. Prospective and retrospective studies assessing progression-free survival (PFS) and overall survival (OS) were included. We used hazard ratio (HR) to determine the value of Deauville criteria and International Harmonization Project (IHP) criteria for measuring survival. Subgroup analysis was performed based on the number of chemotherapy cycles before the mid-term evaluation as well as the visual evaluation method. Results: A total of 11 studies were included. PFS (HR =2.93, 95% confidence interval [CI]: 2.93-3.90, p<0.0001) and OS (HR =2.55, 95% CI: 1.76-3.68, p<0.0001) of PET/CT-positive patients were significantly lower when determined by the visual method. In subgroup analysis, IHP, Deauville criteria, and having no standard interpretation groups were factors able to predict PFS; IHP and having no standard interpretation group were able to predict OS. With PET/CT, IHP, and Deauville 5-point criteria, the PFS of patients receiving 2-4 cycles of chemotherapy before PET/CT was significantly lower than that of PET/CT-negative patients. No significant difference in OS was observed when patients received 3 or fewer cycles of chemotherapy before PET/CT, though OS was significantly lower in patients receiving more than 3 chemotherapy cycles. Conclusion: IHP and Deauville criteria are commonly used for PET/CT visual evaluation at present. Interim PET/CT analysis after 3-4 chemotherapy cycles is capable of predicting disease prognosis. Large-scale prospective clinical trials are needed to confirm whether PET/CT analysis can be used as an indication for changing a treatment strategy.

16.
J Cancer ; 8(17): 3522-3530, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29151937

RESUMO

Background: Breast cancer (BC)-derived hyaluronan (HA) can induce the formation of M2-like tumor-associated macrophages (TAMs) in tumor context. However, little is known about the correlation between circulating M2-like monocytes and plasma HA in BC patients. This study focused on evaluating the relationship between circulating M2-like monocytes and plasma HA, and further appraised the diagnostic value of them in BC. Methods: The expression of M2-like TAMs and HA was determined in pathological tissues by immunohistochemistry. Flow cytometry was used to detect the levels of circulating CD14+CD204+ M2-like monocytes in 81 BC patients, 45 patients with breast benign diseases, and 46 healthy subjects. The levels of HA, CEA, and CA15-3 were measured in plasma samples using chemiluminescence method. Results: M2-like TAMs and HA expressions were elevated in BC tissues compared with benign tissues. In correspondence, the frequency of circulating CD14+CD204+ M2-like monocytes and the plasma HA levels were significantly higher in patients with BC than those in control groups. Importantly, there was a positive correlation between circulating M2-like monocytes and the plasma HA (Spearman r = 0.404, p < 0.001). Area under receiver operating characteristic curve (ROC) for the combination of circulating M2-like monocytes and HA was 0.899 (95% CI: 0.853-0.946), which was higher than the panel of CEA and CA15-3. Conclusions: The frequency of circulating CD14+CD204+ M2-like monocytes was positively correlated to plasma HA levels. The combination of circulating CD14+CD204+ M2-like monocytes and plasma HA could provide considerable diagnostic value in BC.

17.
Appl Microbiol Biotechnol ; 101(21): 8007-8014, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28948330

RESUMO

Nitrite-dependent anaerobic methane oxidation (N-DAMO), which couples anaerobic methane oxidation and nitrite reduction, is a recently discovered bioprocess coupling microbial nitrogen and carbon cycles. The discovery of this microbial process challenges the traditional knowledge of global methane sinks and nitrogen losses. In this study, the abundance and activity of N-DAMO bacteria were investigated and their contributions to methane sink and nitrogen loss were estimated in different seasons and different partitions of an intertidal zone of the East China Sea. The results showed that N-DAMO bacteria were extensively and continuously present in the intertidal zone, with the number of cells ranging from 5.5 × 104 to 2.8 × 105 copy g-1 soil and the potential activity ranging from 0.52 to 5.7 nmol CO2 g-1 soil day-1, contributing 5.0-36.6% of nitrite- and sulfate-dependent anaerobic methane oxidation in the intertidal zone. The N-DAMO activity and its contribution to the methane consumption were highest in the spring and in the low intertidal zone. These findings showed that the N-DAMO process is an important methane and nitrogen sink in the intertidal zone and varies with the seasons and the partitions of the intertidal zone.


Assuntos
Metano/metabolismo , Methylococcaceae/isolamento & purificação , Methylococcaceae/metabolismo , Nitritos/metabolismo , Microbiologia do Solo , Anaerobiose , Carga Bacteriana , China , Methylococcaceae/classificação , Oceanos e Mares , Estações do Ano , Análise Espaço-Temporal
18.
Tumour Biol ; 39(6): 1010428317711571, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28639912

RESUMO

M2-like tumor-associated macrophages promote breast tumor growth and survival and may migrate into the peripheral blood. However, the frequency of circulating M2-like monocytes in the peripheral blood of breast cancer patients has not been clarified. The objective of this study was to determine the percentages of circulating M2-like monocytes in patients with breast cancer. Immunofluorescence staining for CD68 and CD163 was performed to detect M2-like macrophages in pathological tissues. Flow cytometry was used to assess the frequencies of circulating CD14+CD163+/CD14+CD204+/CD14+CD163+CD204+ M2-like monocytes in 99 breast cancer patients, 56 patients with benign breast disease, and 60 healthy controls. Receiver operating characteristic curve analysis was used to compare the diagnostic values of circulating M2-like monocytes, carcinoembryonic antigen, and cancer antigen 15-3. The associations among circulating M2-like monocytes and clinical breast cancer parameters were analyzed. The number of CD68+CD163+ M2-like macrophages was significantly higher in breast cancer tissues than in benign tissues. In the peripheral blood, CD14+CD163+/CD14+CD204+/CD14+CD163+CD204+ M2-like monocytes were elevated in breast cancer patients compared with normal controls and patients with benign breast disease. The area under the receiver operating curve for circulating CD14+CD163+CD204+ M2-like monocytes was 0.888 (95% confidence interval: 0.839-0.936), a value higher than those for carcinoembryonic antigen and cancer antigen 15-3. High frequencies of circulating CD14+CD204+ and CD14+CD163+CD204+ M2-like monocytes were associated with tumor-node-metastasis stage, lymph node metastasis, histological differentiation, and estrogen receptor expression. Circulating M2-like monocytes may serve as a diagnostic biomarker in breast cancer and have a potential role in reflecting breast cancer progression.


Assuntos
Biomarcadores Tumorais/sangue , Neoplasias da Mama/sangue , Receptores de Lipopolissacarídeos/sangue , Macrófagos/metabolismo , Glicoproteínas de Membrana/sangue , Receptores Depuradores/sangue , Receptores Depuradores Classe A/sangue , Adulto , Idoso , Neoplasias da Mama/patologia , Progressão da Doença , Feminino , Citometria de Fluxo , Humanos , Metástase Linfática , Macrófagos/patologia , Pessoa de Meia-Idade , Monócitos/metabolismo , Monócitos/patologia , Receptores de Superfície Celular
19.
Acta Biochim Biophys Sin (Shanghai) ; 49(8): 737-743, 2017 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-28655144

RESUMO

Serum carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is dysregulated in various malignant tumors and has been associated with tumor progression. However, the expression and regulatory mechanisms of serum CEACAM1 in gastrointestinal cancer are still unclear. The expression ratio of the CEACAM1-L and CEACAM1-S isoforms has seldom been investigated in gastrointestinal cancer. In this study, we intended to explore the expression and diagnostic value of CEACAM1 in gastrointestinal cancer. Serum CEACAM1 levels were measured by enzyme-linked immunosorbent assay. The protein expression and distribution of CEACAM1 in tumors were examined by immunohistochemical staining. The expression patterns and ratio of CEACAM1-L/S were analyzed by reverse transcription-polymerase chain reaction. The results showed that serum CEACAM1 levels were significantly higher in cancer patients than in healthy controls. CEACAM1 was found in secreted forms within the neoplastic glands, and its expression was more intense at the tumor invasion front. The CEACAM1-L/S (L:S) ratios were up-regulated during tumorigenesis. Our data suggest that the serum level of CEACAM1 may be used to discriminate gastrointestinal cancer patients from health controls.


Assuntos
Antígenos CD/genética , Moléculas de Adesão Celular/genética , Neoplasias Gastrointestinais/genética , Perfilação da Expressão Gênica/métodos , Regulação Neoplásica da Expressão Gênica , Regulação para Cima , Adulto , Idoso , Idoso de 80 Anos ou mais , Antígenos CD/sangue , Antígenos CD/metabolismo , Moléculas de Adesão Celular/sangue , Moléculas de Adesão Celular/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Neoplasias Gastrointestinais/sangue , Neoplasias Gastrointestinais/metabolismo , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Isoformas de Proteínas/sangue , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa
20.
Mater Sci Eng C Mater Biol Appl ; 71: 1086-1089, 2017 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-27987663

RESUMO

An electrochemical sensor for determining dopamine was developed by modifying phytic acid/graphene oxide (PA/GO) nanocomposites onto a glassy carbon electrode (GCE). PA functionalized GO was prepared by an ultra-sonication method. Subsequently, the PA/GO nanocomposites were drop-casted on a glassy carbon substrate. The structural feature of the PA/GO modified GCE was confirmed by attenuated total reflection infrared (ATR-IR) spectroscopy. The proposed electrochemical sensor was applied to detect various concentrations of DA by differential pulse voltammetry (DPV). The PA/GO/GCE was considered to be highly sensitive to DA in the range of 0.05-10µM. In addition, the PA/GO/GCE demonstrated high electrochemical selectivity toward DA in the presence of ascorbic acid (AA) and uric acid (UA). The prepared electrochemical DA sensor was applied for detection of DA in dopamine hydrochloride injection and spiked samples of human urine with satisfactory results.


Assuntos
Dopamina/urina , Técnicas Eletroquímicas/métodos , Grafite/química , Nanocompostos/química , Ácido Fítico/química , Eletrodos , Humanos
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