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1.
IEEE Trans Image Process ; 29: 29-43, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31170069

RESUMO

Collective activity recognition, which tells what activity a group of people is performing, is a cutting-edge research topic in computer vision. Different from action performed by individuals, collective activity needs to consider the complex interactions among different people. However, most previous works require exhaustive annotations such as accurate label information of individual actions, pairwise interactions, and poses, which could not be easily available in practice. Moreover, most of them treat human detection as a decoupled task before collective activity recognition and leverage all detected persons. This not only ignores the mutual relation between the two tasks, which makes it hard for filtering out irrelevant people, but also probably increases the computation burden when reasoning the collective activities. In this paper, we propose a fast weakly supervised deep learning architecture for collective activity recognition. For fast inference, we propose to make the actor detection and weakly supervised collective activity reasoning collaborate in an end-to-end framework by sharing convolutional layers between them. The joint learning makes the two tasks united and reinforced each other, so that it is more effective to filter out the outliers who are not involved in the activity. For the weakly supervised learning, we propose a latent embedding scheme for mining person-group interactive relationship to get rid of the use of any pairwise relation between people and the individual action labels as well. The experimental results show that the proposed framework achieves comparable or even better performance as compared to the state-of-the-art on three datasets. Our joint modelling reasons collective activities at the speed of 22.65 fps, which is the fastest ever known and substantially makes collective activity recognition more towards real-time applications.

2.
J Integr Neurosci ; 18(3): 327-339, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31601083

RESUMO

Various bioactive substances isolated from natural products play a pivotal role in the prevention and cure of neurodegenerative diseases, such as Alzheimer's disease. Currently, there are many theories about the pathogenesis of this disease. In this review we discuss among them, the cholinergic hypotheses, the Aß toxicity hypothesis, and the tau dysfunction hypothesis. Multiple potential targets are a focus for the development of anti-AD drugs. There is an urgent need to develop more effective therapies to treat and delay the onset of the disease and to find safe and effective drugs. In this review, the recent progress of anti-AD effects and their principal targets are updated.

3.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 50(4): 551-555, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31642234

RESUMO

OBJECTIVE: To investigate the status and influencing factors of the knowledge and behavior related to brucellosis among occupational workers in Jianyang city, and to provide basic information for developing regional specific strategies for brucellosis prevention and control. METHODS: With multistage cluster random sampling, occupational workers aged ≥18 yr. who had been in contact with sheep and their products were interviewed through a questionnaire about demographic characteristic, knowledge, and behavior related to brucellosis. The knowledge and practice scores were described as frequency and percentage, and the awareness rate of knowledge about brucellosis was calculated. Unconditional logistic regression model was used to analyze the influencing factors of knowledge and behavior related to brucellosis. RESULTS: In total, 378 workers were recruited. The minority (25.1%) of the workers had heard of brucellosis, and the awareness rate of brucellosis was 22.0%. Occupational protective behaviors were poor, and the scoring rates of wearing protective clothes, mask and gloves were 58.7%, 76.5%, 71.7% respectively. The results of multivariate logistic regression analysis revealed that the awareness rate of knowledge about brucellosis decreased with age, while the low educational level was found to be associated with low awareness of knowledge about brucellosis, and occupation type had association with the awareness rate of knowledge. Besides, the awareness of knowledge about brucellosis was a protective factor and occupation type were associated with the behavior related to brucellosis. CONCLUSIONS: The knowledge and behavior related to brucellosis are poor among occupational workers in Jianyang. It is necessary to continuously implement targeted health education and health promotion programs in this region, and the people with low education level and livestock farmers should be paid special attention to.

4.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(5): 1497-1503, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31607304

RESUMO

OBJECTIVE: To investigate the effect of baicalin derivative 02-036 on proliferation and apoptosis human Burkitt lymphoma cell line CA46 and its related mechanisms. METHODS: The MTT assay and cell colony formation assay were used to measure the growth inhibition of CA46 cells after 02-036 treatment. The flow cytometry with AnnexinV-FITC/PI double staining was employed to detect the apoptosis induction effect of 02-036 on CA46 cells. Cell cycle distribution of CA46 cells was estimeted by using DNA ploid analysis. Western blot was used to determine the changes of apoptosis-related proteins, including C-MYC, BCL-2, Procaspase-9, Procaspase-3, PARP and Cleaved-PARP. RESULTS: Baicalin derivative 02-036 obviously inhibited the proliferation of CA46 cells, with dose- and time-dependent manner (r=0.963, r=0.992). The averaged IC50 value of CA46 cells was (6.04±0.11) µmol/L after 48-hour treatment. Low concentration of 02-036 could significantly inhibit the colony formation of CA46 cells. Flow cytometry analysis confirmed that 02-036 could effectively induce CA46 cell apoptosis. The apoptosis rate correlated with drug concentrations (r=0.959). Also, DNA ploid analysis showed that the cell cycle of CA46 was arrested in the S phase. The expression levels of BCL-2, Pro-caspase-9, Pro-caspase-3, PARP and C-MYC proteins decreased with a 02-036-dose dependent manner (r values were -0.990, -0.939, -0.971 and -0.967, respectively). In contrast, the expression level of cleaved-PARP increased with the same manner (r=0.920). CONCLUSION: Baicalin derivative 02-036 can effectively inhibit the proliferation and induce apoptosis of CA46 cells, and its related mechanisms may be correlated with the down-regulation of apoptosis-related molecule expression levels, such as BCL-2, Pro-caspase-9, Pro-caspase-3, PARP and C-MYC.

6.
World J Surg ; 2019 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-31637507

RESUMO

BACKGROUND: The prognostic significance of preoperative plasma fibrinogen in patients with operable gastric cancer remains under debate. This study aimed to elucidate the prognostic value of fibrinogen in gastric cancer patients underwent gastrectomy. METHODS: A total of 4351 patients with gastric cancer collected from three comprehensive medical centers were retrospectively evaluated. Patients were categorized by minimum P value using X-tile, while the baseline confounders for fibrinogen was balanced through propensity score matching (PSM). The relationships between fibrinogen and other clinicopathologic features were evaluated, and nomogram was constructed to assess its prognostic improvement compared with TNM staging system. RESULTS: Fibrinogen was significantly correlated with macroscopic type, tumor differentiation, tumor size, and T and N stage. The factors, fibrinogen and T stage as well as N stage, were identified to be independent prognostic factors after PSM. Nomogram based on fibrinogen demonstrated a smaller Akaike information criterion (AIC) and a larger concordance index (C-index) than TNM staging system, illustrating that fibrinogen might be able to improve the prognostic accuracy. CONCLUSIONS: Preoperative plasma fibrinogen levels in gastric cancer patients were significantly correlated with tumor progression, which could be regarded as a reliable marker for survival prognostic prediction.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31637618

RESUMO

Vermi-composting is considered to be a feasible method for reducing tetracycline resistance genes (TRGs) in the sludge. Nevertheless, the way different gut passages of earthworm might affect the fates of TRGs and whether this process is affected by tetracycline (TC) concentrations need to be further investigated. In this study, we examined the effects of TC concentrations on changes in TRGs and bacterial communities in gut passages of earthworm were determined by using quantitative PCR and Illumina high-throughput sequencing. TRGs and intI1 were mainly reduced in the hindgut under the TC concentrations ranging from 0 to 25 mg/kg, while they were enriched under higher TC stress exposure. Consequently, we suggest the TC limitation of 25 mg/kg in the domestic sludge (DS) for vermi-composting. Although the predominant genera were TC sensitive under TC stress, many bacterial hosts harboring multiple TRGs (especially those in the hindgut) should be paid further attention to. In the foregut, five genera with abundant tetracycline-resistant bacteria (TRB) were specialized taxa. Among these genera, Unclassified_Solirubrobacterales and Pirellulaceae were probably related to the digestion processes. Other unclassified taxa related to the TRGs were probably derived from the DS. Five genera with abundant TRB were shared in the gut passages, and three specialized genera in the hindgut. These genera could spread TRGs and intI1 to the environment. These results suggest that vermi-composting is a feasible approach for TRG control in the DS containing TC concentration that does not exceed 25 mg/kg. Fates of TRGs and intI1 widely differ in the gut passages, showing inevitable connections with bacterial communities.

8.
J Clin Neurosci ; 2019 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-31630917

RESUMO

Little information is available on associations between different lumbar interbody fusion (LIF) surgeries and postoperative outcomes. The present study aims to comprehensively investigate whether different LIF techniques are associated with postoperative outcomes such as complications and length of hospital stay. The United States Nationwide Inpatient Sample (NIS) was searched for patients diagnosed with recurrent lumbar disc herniation who underwent lumbar interbody fusion (LIF) surgeries between 2005 and 2014. Patients were categorized based on LIF approaches: anterior lumbar interbody fusion (ALIF); lateral lumbar interbody fusion (LLIF); or posterior lumbar interbody fusion/transforaminal lumbar interbody fusion (PLIF/TLIF). A total of 2625 patients were included in this study. After adjusting for age, severity of illness, and comorbidities, patients who received LLIF and PLIF/TLIF approaches had significantly shorter hospital stays than those receiving ALIF (LLIF vs. ALIF, ß = -0.64; PLIF/TLIF vs. ALIF, ß = -0.40). In addition, patients who received LLIF and PLIF/TLIF approaches had significantly lower risk of digestive system complications compared to those receiving ALIF (LLIF vs. ALIF, aOR = 0.25; PLIF/TLIF vs. ALIF, aOR = 0.18). In conclusion, in patients with recurrent lumbar disc herniation, LLIF and PLIF/TLIF approaches are associated with shorter hospital stays and lower risk of digestive system complications than ALIF. However, LIF approaches do not correlate significantly with the risk of postoperative bleeding or nervous system complications.

9.
J Nanobiotechnology ; 17(1): 102, 2019 Oct 03.
Artigo em Inglês | MEDLINE | ID: mdl-31581945

RESUMO

Natural extracellular matrices (ECMs) are three-dimensional (3D) and multi-scale hierarchical structure. However, coatings used as ECM-mimicking structures for osteogenesis are typically two-dimensional or single-scaled. Here, we design a distinct quasi-three-dimensional hierarchical topography integrated of density-controlled titania nanodots and nanorods. We find cellular pseudopods preferred to anchor deeply across the distinct 3D topography, dependently of the relative density of nanorods, which promote the osteogenic differentiation of osteoblast but not the viability of fibroblast. The in vivo experimental results further indicate that the new bone formation, the relative bone-implant contact as well as the push-put strength, are significantly enhanced on the 3D hierarchical topography. We also show that the exposures of HFN7.1 and mAb1937 critical functional motifs of fibronectin for cellular anchorage are up-regulated on the 3D hierarchical topography, which might synergistically promote the osteogenesis. Our findings suggest the multi-dimensions and multi-scales as vital characteristic of cell-ECM interactions and as an important design parameter for bone implant coatings.

10.
Curr Med Sci ; 39(5): 685-689, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31612383

RESUMO

B cells are a heterogeneous population, which have distinct functions of antigen presentation, activating T cells, and secreting antibodies, cytokines as well as protease. It is supposed that the balance among these B cells subpopulation (resting B cells, activated B cells, Bregs, and other differentiated B cells) will determine the ultimate role of B cells in tumor immunity. There has been increasing evidence supporting opposite roles of B cells in tumor immunity, though there are no general acceptable phenotypes for them. Recent years, a new designated subset of B cells identified as Bregs has emerged from immunosuppressive and/or regulatory functions in tumor immune responses. Therefore, transferring activated B cells would be possible to become a promising strategy against tumor via conquering the immunosuppressive status of B cells in future. Understanding the potential mechanism of double-edge role of B cells will help researchers utilize activated B cells to improve their anti-tumor response. Moreover, the molecular pathways related to B cell differentiation are involved in its tumor-promoting effect, such as NF-κB, STAT3, BTK. So, we review the molecular and signaling pathway mechanisms of B cells involved in both tumor-promoting and tumor-suppressive immunity, in order to help researchers optimize B cells to fight cancer better.

11.
Environ Pollut ; 255(Pt 2): 113296, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31600706

RESUMO

Airborne (PM2.5) with aerodynamic diameter ≤ 2.5 µm was collected from 4 types of cities in northeast China during the heating period. The objectives of this study were to assess the concentrations variation of PM2.5-bound 12 carcinogenic polycyclic aromatic hydrocarbons (PAH12), to study the influence of simulated lung fluids on bioaccessibility of PAH12 and to estimate the variation of lifetime excess cancer risk to the residents, artificial lysosomal fluid (ALF) and Gamble's solution were used. The number of lifetime excess cancer cases (determined by California Environmental Protection Agency method) as a result of PAH12 exposure (total concentration) was 4.00-430 (provincial central cities), 24.0-261 (energy-mining cities), 17.0-109 (forested city), and 20.0-69.0 (agricultural city) per million people, which relatively corresponded to a 92.2% and 96.2%, 92.6% and 97.3%, 92.2% and 94.2%, and 86.5% and 92.6% decrease after considering bioaccessibility following 24-h of Gamble's solution and ALF extraction, respectively. Phenanthrene (Phe), dibenz[a,h]anthracene (DahA) and benzo[a]pyrene (BaP) were found to be the most bioaccessible types of PAH12 after the Gamble's solution and ALF extraction in the PM2.5 samples from all the studied cities. Based on the point-estimate approach, short-term predictions of pulmonary toxicity caused by potential inhalation of airborne PM into the pulmonary system might be overestimated if bioaccessibility of PM2.5-bound PAH12 is not fully evaluated.

12.
Chin Med J (Engl) ; 132(18): 2157-2167, 2019 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-31490265

RESUMO

BACKGROUND: Handan Eye Study (HES), a large population-based cohort study in rural area of northern China, was one of the few studies focusing on the major eye diseases of rural Chinese population. The aim of this study was to introduce the design, methodology and to assess the data quality of the follow-up phase of HES. METHODS: All participants were recruited in Yongnian county of Handan city between 2012 and 2013. Main outcomes were measured by visual quality scales and ocular examinations. We performed the Chi-square test to make comparison of categorical data among groups, One-way analysis of variance and Kruskal-Wallis test was applied to make comparison of continuous data among groups, a post-hoc test was done to make further pairwise comparison. Inter-class correlation coefficients (ICCs) and Kappa coefficients were used to evaluate the consistency between different operators. Logistic regression was used to explore the influence factors of death, odds ratio (OR) and 95% confidence interval (CI) were used to estimate the effect size of each influence factor. RESULTS: The follow-up rate was 85.3%. Subjects were classified into three groups: the follow-up group (n = 5394), the loss to follow-up group (n = 929), and the dead group (n = 507), comparison of their baseline information was done. Compared with the other two groups, age of the dead group (66.52 ±â€Š10.31 years) was the oldest (Z = 651.293, P < 0.001), male proportion was the highest (59.0%) (χ = 42.351, P < 0.001), only 65.9% of the dead finished middle school education (Z = 205.354, P < 0.001). The marriage percentage, body mass index (BMI), best-corrected visual acuity (BCVA), and intra-ocular pressure of the dead group was the lowest either. Spherical equivalent error (SER) of the dead group was the highest. Besides, history of smoking, hypertension, diabetes, and heart disease were more common in the dead group. Multivariate analysis showed that age (OR = 1.901, 95% CI: 1.074-1.108), gender (OR = 0.317, 95% CI: 0.224-0.448), and BCVA (OR = 0.282, 95% CI: 0.158-0.503) were associated with death. While between the follow-up group and the loss to follow-up group, there was only difference on age, gender, BMI, systolic blood pressure and SER. The Cronbach coefficients of all scales used in the follow-up were ≥0.63 and the cumulative variances were ≥0.61, indicating good reliability and validity. The ICCs and Kappa coefficients between different operators were ≥0.69. CONCLUSIONS: HES has a high follow-up rate and a low risk of loss to follow-up bias. Age, gender, and BCVA are influence factors of death. Specifically, male subjects are at a higher risk of death than female, age is a risk factor of death while BCVA is a protective factor for death.

15.
Inorg Chem ; 58(18): 12385-12394, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31486636

RESUMO

Gibbsite (α-Al(OH)3) transformation into layered double hydroxides, such as lithium aluminum hydroxide dihydrate (LiAl-LDH), is generally thought to occur by solid-state intercalation of Li+, in part because of the intrinsic structural similarities in the quasi-2D octahedral Al3+ frameworks of these two materials. However, in caustic environments where gibbsite solubility is high relative to LiAl-LDH, a dissolution-reprecipitation pathway is conceptually enabled, proceeding via precipitation of tetrahedral (Td) aluminate anions (Al(OH)4-) at concentrations held below 150 mM by rapid LiAl-LDH nucleation and growth. In this case, the relative importance of solid-state versus solution pathways is unknown because it requires in situ techniques that can distinguish Al3+ in solution and in the solid phase (gibbsite and LiAl-LDH), simultaneously. Here, we examine this transformation in partially deuterated LiOH solutions, using multinuclear, magic angle spinning, and high field nuclear magnetic resonance spectroscopy (27Al and 6Li MAS NMR), with supporting X-ray diffraction and scanning electron microscopy. In situ 27Al MAS NMR captured the emergence and decline of metastable aluminate ions, consistent with dissolution of gibbsite and formation of LiAl-LDH by precipitation. High field, ex situ 6Li NMR of the the progressively reacted solids resolved an Oh Li+ resonance that narrowed during the transformation. This is likely due to increasing local order in LiAl-LDH, correlating well with observations in high field, ex situ 27Al MAS NMR spectra, where a comparatively narrow LiAl-LDH Oh 27Al resonance emerges upfield of gibbsite resonances. No intermediate pentahedral Al3+ is resolvable. Quantification of aluminate ion concentrations suggests a prominent role for the solution pathway in this system, a finding that could help improve strategies for manipulating Al3+ concentrations in complex caustic waste streams, such as those being proposed to treat the high-level nuclear waste stored at the U.S. Department of Energy's Hanford Nuclear Reservation in Washington State, USA.

16.
ACS Appl Mater Interfaces ; 11(42): 38689-38696, 2019 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-31503448

RESUMO

Mg batteries have been proposed as potential alternatives to lithium-ion batteries because of their lower cost, higher safety, and enhanced charge density. However, the Mg metal readily oxidizes when exposed to an oxidizer to form a thin MgO passivation surface layer that blocks the transport of Mg2+ across the solid electrode-electrolyte interface (SEI). In this work, the adsorption and thermal decomposition of diglyme (G2) and electrolytes containing Mg(TFSI)2 in G2 on 10 nm-sized MgO particles are evaluated by a combination of in situ 13C single-pulse, surface-sensitive 1H-13C cross-polarization (CP) magic-angle spinning (MAS) nuclear magnetic resonance, and quantum chemistry calculations. At 180 °C, neat G2 decomposes on MgO to form surface-adsorbed -OCH3 groups that are captured as a distinctive peak located at about 50 ppm in the CP/MAS spectrum. At low Mg(TFSI)2 salt concentration, the main solvation structure in this electrolyte is solvent-separated ion pairs without extensive Mg-TFSI contact ion pairs. G2, likely including a small amount of G2-solvated Mg2+, adsorbs onto the MgO surface. At high Mg(TFSI)2 salt concentrations, contact ion pairs between Mg and TFSI are formed extensively in the solution with the first solvation shell containing one pair of Mg-TFSI and two G2 molecules and the second solvation shell containing up to six G2 molecules, namely, MgTFSI(G2)2(G2)6+. In the presence of MgO, MgTFSI(G2)2(G2)6+ adsorbs onto the MgO surface. At 180 °C, the MgO surface stimulates a desolvation process converting MgTFSI(G2)2(G2)6+ to MgTFSI(G2)2+ and releasing G2 molecules from the second solvation shell of the MgTFSI(G2)2(G2)6+ cluster into the solution. MgTFSI(G2)2+ and MgTFSI(G2)2(G2)6+ tightly adsorb onto the MgO surface and are observed by 1H-13C CP/MAS experiments. The results contained herein show that electrolyte composition has a directing role in the species present on the electrode surface, which has implications on the structures and constituents of the solid-electrolyte interface on working electrodes and can be used to better understand its formation and the failure modes of batteries.

17.
Eur J Cancer ; 120: 10-19, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31446212

RESUMO

BACKGROUND: The role of epidermal growth factor receptor (EGFR) pathways in regulating telomerase is increasingly being recognised. We analysed the impact of rs2853669 single nucleotide polymorphism (SNP) on telomere parameters and its prognostic value for non-small cell lung cancer (NSCLC) with or without EGFR mutation. METHODS: The association of rs2853669 with telomerase reverse transcriptase (TERT) mRNA level and relative telomere length (RTL) was analysed using resected tumour samples from 250 NSCLC patients. We also investigated the patients' clinical outcomes with a median follow-up of 57 months (2-99 months). RESULTS: The rs2853669 T/C allele was significantly associated with lower TERT mRNA expression (versus C/C and versus T/T; p < 0.001 for both) and shorter RTL (versus C/C and versus T/T; p = 0.039 and 0.023) in patients without EGFR mutation. Such difference was not observed in their counterparts harbouring EGFR mutation. When considering the cohort as a whole, T/C allele was significantly associated with shortest overall survival compared with T/T or C/C allele (mean: 61.8, 80.9 and 88.7 months, plog-rank < 0.001) and disease-free survival (mean: 78.3, 87.9 and 91.5 months, plog-rank = 0.019). Stratification analyses showed that the negative prognostic effect of T/C on OS was constrained in patients without EGFR mutation. CONCLUSION: Our study revealed significant associations of a common SNP within TERT promoter region on telomere parameters and survival in NSCLC patients without EGFR mutation. The result may help providing instruction for therapeutic interventions targeting telomerase and evidence for investigation of TERT-EGFR interacting mechanism in telomere biology.

18.
Surg Endosc ; 2019 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-31451922

RESUMO

BACKGROUND: Peroral endoscopic myotomy (POEM) is a safe and effective approach for achalasia. However, the safety, feasibility, perioperative and long-term efficacy in treating geriatric patients has not been well evaluated. METHODS: Data of 2367 patients diagnosed with achalasia and treated with POEM in the Endoscopy Center, Zhongshan Hospital, Fudan University from August 2010 to December 2017 were retrospectively reviewed. Last follow-up was in December 2018. Propensity score matching based on baseline characteristics was used to adjust for confounding. With a caliper of 0.01 in propensity scoring, 139 patients aged ≥ 65 years were matched at a 1:2 ratio with 275 patients aged < 65 years. Perioperative complications and long-term outcomes were compared between the two groups. RESULTS: After propensity score matching, the two groups had similar baseline clinical characteristics and distribution of propensity scores. The mean age was 70.22 years in geriatric patients and 42.02 in younger patients. Technical failure occurred in one geriatric and one non-geriatric patients (p = 0.485). The procedural time in geriatric patients was similar to younger patients [50 (interquartile range (IQR) 36-76) vs. 50 (IQR 36-70) min, p = 0.398]. There were also no significant differences in major perioperative adverse events (2.88% vs. 2.18%, p = 0.663) and hospitalization length (median 3 vs. 3 days, p = 0.488). During a median follow-up period of 41 months (IQR 26-60), mean decrease in Eckardt score and pressure of the LES were 6.63 and 11.9 mmHg in geriatric patients, which were similar to the change in non-geriatric patients (6.49 and 11.6 mmHg, p = 0.652 and 0.872, respectively). Clinical reflux occurred in 23.53% geriatric patients and 21.59% non-geriatric patients (p = 0.724). 5-year success rate of 92.94% was achieved in geriatric patients and 92.61% in younger patients (log-rank p = 0.737). CONCLUSIONS: POEM is a safe and reliable treatment in geriatric achalasia patients with confirmed short-term and long-term efficacy compared with those in non-geriatric patients.

19.
Surgery ; 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31378477

RESUMO

BACKGROUND: This study aimed to evaluate the clinical efficacy and safety of endoscopic thoracic sympathicotomy and to explore strategies to decrease the incidence of transfer hyperhidrosis (TH). METHODS: From January 2003 to July 2016, 10,275 patients with primary palmar hyperhidrosis underwent endoscopic thoracic sympathicotomy in 15 different institutions. We carried out a retrospective analysis of these patients who were grouped into group A, those with nonretained R2 (R2, R2-3, or R2-4 ablation), and group B, those with retained R2 (single R3 or R4 ablation). RESULTS: All procedures were performed successfully. Both hands of all patients became warm and dry immediately after endoscopic thoracic sympathicotomy. Pneumothorax occurred in 146 patients, and 39 patients had intraoperative bleeding. Follow-up was carried out from 6 months to 13 years. A total of 531 patients (5.2%) were lost to follow-up. The effective rate for primary palmar hyperhidrosis was 100%. Palmar hyperhidrosis recurred in 73 patients (0.7%). Transfer hyperhidrosis appeared in 7,678 patients (78.8%). For groups A and B, the incidence of TH was 80.4% and 78.5%, respectively (P > .05), but the incidence of grade III+IV TH in group B (1.6%) was less than that in group A (4.8%; P < .001). CONCLUSION: Endoscopic thoracic sympathicotomy is a minimally invasive, safe, and effective therapeutic method for primary palmar hyperhidrosis. Although the overall incidence of TH is high, the incidence of grade III to IV TH can be decreased by reserving R2, lowering the level of thoracic sympathicotomy, and single severing of R3 or R4.

20.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 27(4): 1008-1012, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31418349

RESUMO

OBJECTIVE: To investigate the role of nucleophosmin (NPM) in the proliferation of chronic myeloid leukemia cells (K562 cells) and its mechanism by RNAi technology. METHODS: shRNA was used to inhibit the expression of NPM. The expression of NPM gene was detected by real-time quantitative PCR. The effect of inhibiting NPM gene on cell proliferation was detected by MTS assay. Change of cell cycle was detected by flow cytometry. Western blot was used to detect the expression of cell cycle-related proteins. RESULTS: The shRNA lentiviral vector targeting at NPM gene was successfully constructed and used to transfect the K562 cells. The results showed that compared with the control groups, suppression of NPM gene expression in K562 cells could inhibit the cell proliferation and decrease the cell colony formation. Moreover, interference of NPM gene could prolong G0/G1 phase and arrest cell cycle, which may be related to the down-regulation of NPM gene expression and activation of p21 protein expression, thereby inhibited the formation of CDK2/ Cyclin E complex. CONCLUSION: Down-regulation of NPM gene expression in K562 cells can induce cell cycle arrest and inhibit cell proliferation.


Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva , Apoptose , Proliferação de Células , Técnicas de Silenciamento de Genes , Humanos , Células K562 , Proteínas Nucleares
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