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1.
Chin J Nat Med ; 20(6): 401-420, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35750381

RESUMO

Bacterial surface glycans perform a diverse and important set of biological roles, and have been widely used in the treatment of bacterial infectious diseases. The majority of bacterial surface glycans are decorated with diverse rare functional groups, including amido, acetamidino, carboxamido and pyruvate groups. These functional groups are thought to be important constituents for the biological activities of glycans. Chemical synthesis of glycans bearing these functional groups or their variants is essential for the investigation of structure-activity relationships by a medicinal chemistry approach. To date, a broad choice of synthetic methods is available for targeting the different rare functional groups in bacterial surface glycans. This article reviews the structures of naturally occurring rare functional groups in bacterial surface glycans, and the chemical methods used for installation of these groups.

2.
Sci Total Environ ; 838(Pt 3): 156425, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35660600

RESUMO

BACKGROUND: Composite temperature-related indices have been utilized to comprehensively reflect the impact of multiple meteorological factors on health. We aimed to evaluate the predictive ability of temperature-related indices, choose the best predictor of stroke morbidity, and explore the association between them. METHODS: We built distributed lag nonlinear models to estimate the associations between temperature-related indices and stroke morbidity and then applied two types of cross-validation (CV) methods to choose the best predictor. The effects of this index on overall stroke, intracerebral hemorrhage (ICH), and ischemic stroke (IS) morbidity were explored and we explained how this index worked using heatmaps. Stratified analyses were conducted to identify vulnerable populations. RESULTS: Among 12 temperature-related indices, the alternative temperature-humidity index (THIa) had the best overall performance in terms of root mean square error when combining the results from two CVs. With the median value of THIa (25.70 °C) as the reference, the relative risks (RRs) of low THIa (10th percentile) reached a maximum at lag 0-10, with RRs of 1.20 (95%CI:1.10-1.31), 1.49 (95%CI:1.29-1.73) and 1.12 (95%CI:1.03-1.23) for total stroke, ICH and IS, respectively. According to the THIa formula, we matched the effects of THIa on stroke under various combinations of temperature and relative humidity. We found that, although the low temperature (<20 °C) had the greatest adverse effect, the modification effect of humidity on it was not evident. In contrast, lower humidity could reverse the protective effect of temperature into a harmful effect at the moderate-high temperature (24 °C-27 °C). Stratification analyses showed that the female was more vulnerable to low THIa in IS. CONCLUSIONS: THIa is the best temperature-related predictor of stroke morbidity. In addition to the most dangerous cold weather, the government should pay more attention to days with moderate-high temperature and low humidity, which have been overlooked in the past.


Assuntos
Acidente Vascular Cerebral , China/epidemiologia , Feminino , Humanos , Umidade , Morbidade , Acidente Vascular Cerebral/epidemiologia , Temperatura
3.
Forensic Sci Int ; 337: 111350, 2022 May 26.
Artigo em Inglês | MEDLINE | ID: mdl-35709589

RESUMO

Drug abuse/misuse is now a major global problem affecting public health as well as economic and social stability. This study presents a retrospective view of the prevalence of drugs in hair. Reasons for hair testing include the need for law enforcement to test suspicious individuals and the need for employers in specific industries to test their employees. Toxicology analysis results were reviewed for a total of 19,275 hair samples requested for drug abuse/misuse analysis at the Academy of Forensic Science over 29 months from February 2019 to June 2021. Drugs and their metabolites in hair samples were analyzed by liquid chromatography - tandem mass spectrometry. In the study, the 19,275 hair samples were screened for a total of 62 illegal drugs, which were divided into three categories: illegal-drugs (I), medication (II), and new psychoactive substances (III). These three categories contain 11, 29 and 22 drugs respectively. In these hair samples, 4852 (25.2 %) tested positive for one or more drugs. Among them, the positive rate of category I was the highest (48.7 %), followed by category II (28.5 %), and category III (22.7 %). Over all, the positive rate of male users (26.2 %) was higher than that of female users (23.7 %), and most of them were young people (25-44 years old). Illegal-drugs (morphine, etc.) had the highest positive rate. Under the influence of some certain factors, the drug abuse situation will also undergo corresponding changes. The results can provide a scientific knowledge database which can help in the prevention of drug abuse.

4.
Ultrason Sonochem ; 87: 106057, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35679807

RESUMO

The evolution of acoustic Lichtenberg figure (ALF) in ultrasound fields is studied using high-speed photography. It is observed that bubbles travel along the branch to the aggregation region of an ALF, promoting the possibility of large bubble or small cluster formation. Large bubbles move away from the aggregation region while surrounding bubbles are attracted into this structure, and a bubble transportation cycle arises in the cavitation field. A simplified model consisting of a spherical cluster and a chain of bubbles is developed to explain this phenomenon. The interaction of the two units is analyzed using a modified expression for the secondary Bjerknes force in this system. The model reveals that clusters can attract bubbles on the chain within a distance of 2 mm, leading to a bubble transportation process from the chain to the bubble cluster. Many factors can affect this process, including the acoustic pressure, frequency, bubble density, and separation distance. The larger the bubble in the cluster, the broader the attraction region. Therefore, the presence of large bubbles might enhance the process in this system. Local disturbances in bubble density could destroy the ALF structure. The predictions of the model are in good agreement with the experimental phenomena.


Assuntos
Acústica , Fotografação
5.
Artigo em Inglês | MEDLINE | ID: mdl-35727737

RESUMO

CONTEXT: Osteogenesis imperfecta (OI) is a rare, heterogeneous genetic disorder characterized by bone fragility and recurrent fractures. Bisphosphonates (BPs) are the most commonly used medications for OI, but their efficacy has great variability. OBJECTIVE: We investigate the relationship of pathogenic gene mutations and responses to zoledronic acid (ZOL) in a large cohort of OI children. METHODS: Children with OI were included who received ZOL treatment and followed up for at least one year. Bone mineral density (BMD), serum levels of ß-isomerized carboxy-telopeptide of type I collagen (ß-CTX, bone resorption marker) were measured at baseline and during follow-up. Causative mutations of OI were identified using next-generation sequencing and Sanger sequencing. RESULTS: 201 OI children were included who initiated ZOL treatment at a median age of 5 years, with mutations identified in 11 genes. After 3 years of treatment, the increase of femoral neck BMD Z-score in OI patients with autosomal dominant inheritance (AD) was greater than that in patients with autosomal recessive or X-linked inheritance (non-AD) (4.5±2.9 vs. 2.0±1.0, P<0.001). Collagen structural defects were negatively correlated with the increase of femoral neck BMD Z-score. Patients with collagen structural defects had higher incidence of new fracture (35.1% vs. 18.4%, Relative risk 0.52, P=0.044) and less decline in ß-CTX level than those with collagen quantitative reduction. Increase in lumbar spine BMD as well as change in height Z-score was not associated with the genotype of OI children. CONCLUSION: OI patients with non-AD inheritance or with pathogenic mutations leading to collagen structural defects may have relatively poor responses to ZOL treatment, which is possibly associated with their more severe phenotypes. New therapeutic agents are worth developing in these patients.

6.
Front Neurosci ; 16: 898902, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35663555

RESUMO

Few researchers investigated the topological properties and relationships with cognitive deficits in sudden sensorineural hearing loss (SNHL) with tinnitus. To explore the topological characteristics of the brain connectome following SNHL from the global level and nodal level, we recruited 36 bilateral SNHL patients with tinnitus and 37 well-matched healthy controls. Every subject underwent pure tone audiometry tests, neuropsychological assessments, and MRI scanning. AAL atlas was employed to divide a brain into 90 cortical and subcortical regions of interest, then investigated the global and nodal properties of "small world" network in SNHL and control groups using a graph-theory analysis. The global characteristics include small worldness, cluster coefficient, characteristic path length, local efficiency, and global efficiency. Node properties include degree centrality, betweenness centrality, nodal efficiency, and nodal clustering coefficient. Interregional connectivity analysis was also computed among 90 nodes. We found that the SNHL group had significantly higher hearing thresholds and cognitive impairments, as well as disrupted internal connections among 90 nodes. SNHL group displayed lower AUC of cluster coefficient and path length lambda, but increased global efficiency. The opercular and triangular parts of the inferior frontal gyrus, rectus gyrus, parahippocampal gyrus, precuneus, and amygdala showed abnormal local features. Some of these connectome alterations were correlated with cognitive ability and the duration of SNHL. This study may prove potential imaging biomarkers and treatment targets for future studies.

7.
Mol Cell Biochem ; 2022 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-35670901

RESUMO

Hypoxia can cause Epithelial-mesenchymal transition (EMT) in renal tubular cells, and in turn, renal fibrosis. We tested the expression of TRIM46, a member of tripartite motif-containing (TRIM) family proteins, and mesenchymal markers under hypoxia. Our results showed that hypoxia significantly enhanced expression of TRIM46 in HK2 human renal proximal tubular epithelial cells. Our data further showed that hypoxia led to upregulated expression of mesenchymal markers including α-smooth muscle actin, vimentin, and Snail, and downregulated expression of epithelial marker E-cadherin, coupled with an increased abundance of nuclear ß-catenin. However, such effects were reversed when TRIM46 expression was knocked down. TRIM46 overexpression had similar effects as hypoxia exposure, and such effects were reversed when cells were treated with XAV-939, a selective inhibitor for ß-catenin. Furthermore, we found that TRIM46 promoted ubiquitination and proteasomal degradation of Axin1 protein, a robust negative regulator of Wnt/ß-catenin signaling activity. Finally, increased TRIM46 coupled with decreased Axin1 was observed in a rat renal fibrosis model. These data suggest a novel mechanism contributing to EMT that mediates hypoxia-induced renal fibrosis. Our results suggest that selectively inhibiting this pathway that activates fibrosis in human kidney may lead to development of a novel therapeutic approach for managing this disease.

8.
Acta Biomater ; 2022 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-35697198

RESUMO

Very limited treatment options are available to fight hepatocellular carcinoma (HCC), a serious global health concern with high morbidity and mortality. The integration of multiple therapies into one nanoplatform to exert synergistic therapeutic effects offers advantages over monotherapies. Here, we describe the construction of the nanoplatform Sor@GR-COF-366 for synergistic chemotherapy and photodynamic therapy (PDT) for HCC using a porphyrin-based covalent organic framework (COF-366) coated with N-acetyl-galactosamine (GalNAc) and rhodamine B (RhB), and loaded with the first-line agent, Sorafenib (Sor). The nanoplatform is targeted towards ASGPR-overexpressed HCC cells and liver tissues by GalNAc and observed by real-time imaging of RhB in vitro and in vivo. The nanoplatform Sor@GR-COF-366 exerts an enhanced synergistic tumor suppression effect in a subcutaneous HCC mouse model with a tumor inhibition rate (TGI) of 97% while significantly prolonging survival at very low toxicity. The potent synergistic therapeutic outcome is confirmed in an orthotopic mouse model of HCC with the TGI of 98% with a minimally invasive interventional PDT (IPDT). Sor@GR-COF-366 is a promising candidate to be combined with chemo-IPDT for the treatment of HCC. STATEMENT OF SIGNIFICANCE: This work describes the construction of covalent-organic frameworks (COFs) modified with glyco-moieties to serve as hepato-targeted multitherapy delivery systems. They combine minimally invasive interventional photodynamic therapy (IPDT) triggered synergism with chemotherapy treatment for hepatocellular carcinoma (HCC). With the aid of minimally invasive intervention, PDT can elicit potent anti-cancer activity for deep solid tumors. This platform shows strong therapeutic outcomes in both subcutaneous and orthotopic mouse models, which can significantly prolong survival. This work showed an effective combination of a biomedical nano-formulation with the clinical operational means in cancer treatment, which is greatly promising in clinical translation.

9.
Ann Neurol ; 2022 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-35700120

RESUMO

OBJECTIVE: Oculopharyngodistal myopathy (OPDM) is an adult-onset neuromuscular disease characterized by progressive ptosis, dysarthria, ophthalmoplegia, and distal muscle weakness. Recent studies revealed GGC repeat expansions in 5'-UTR of LRP12, GIPC1, and NOTCH2NLC are associated with OPDM. Despite these advances, around 30% of OPDM patients remain genetically undiagnosed. Herein, we aim to investigate genetic basis for undiagnosed OPDM patients in two unrelated Chinese Han families. METHODS: Parametric linkage analysis was performed. Long-read sequencing followed by repeat-primed polymerase chain reaction (RP-PCR) and amplicon length polymerase chain reaction (AL-PCR) were used to determine the genetic cause. Targeted methylation sequencing was implemented to detect epigenetic changes. The possible pathogenesis mechanism was investigated by qPCR, immunoblotting, RNA FISH, and immunofluorescence staining of muscle biopsy samples. RESULTS: The disease locus was mapped to 12q24.3. Subsequently, GGC repeat expansion in the promoter region of RILPL1 was identified in six OPDM patients from two families, findings consistent with a founder effect, designated as OPDM type 4 (OPDM4). Targeted methylation sequencing revealed hypermethylation at RILPL1 locus in unaffected individuals with ultralong expansion. Analysis of muscle samples showed no significant differences in RILPL1 mRNA or RILPL1 protein levels between patients and controls. Public CAGE-seq data indicated that alternative TSSs exist upstream of the RefSeq-annotated RILPL1 TSS. Strand-specific RNAseq data revealed bidirectional transcription from the RILPL1 locus. Finally, FISH/IF indicated that both sense and antisense transcripts formed RNA foci and were co-localized with hnRNPA2B1 and p62 in the intranuclear inclusions of OPDM4 patients. INTERPRETATION: Our findings implicate abnormal GGC repeat expansions in the promoter region of RILPL1 as a novel genetic cause for OPDM, and suggest a methylation mechanism and a potential RNA toxicity mechanism are involved in OPDM4 pathogenesis. This article is protected by copyright. All rights reserved.

10.
Front Pharmacol ; 13: 888684, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35677425

RESUMO

Lung infection is a common complication induced by stroke and seriously affects the prognosis and life quality of patients. However, effective therapeutic strategies are still lacking. In the present study, the herb formula GCis was confirmed to prevent pulmonary infection induced by intracerebral hemorrhage (ICH). The animal model of lung infection induced by ICH, GCis (Ginseng Radix et Rhizoma, Aconiti Lateralis Radix Praeparata, and Cistanches Herba) was orally administrated every day for 7 days. Lung microbial biomass and pathological results showed that the GCis formula pretreatment significantly reduced lung bacterial biomass and alleviated pathological abnormalities. These results indicated that the GCis formula has a clear pharmacological effect on preventing lung infection induced by ICH. Immunosuppression induced by ICH seemed to be the main mechanism of lung infection. Our results showed that the spleen and thymus indexes, WBC, and LY% contents were significantly increased in the GCis formula group. Moreover, bone marrow cells were further analyzed by transcriptome sequencing, and GO and KEGG enrichment analysis results showed that immune function was the main pathway enriched by differential genes after GCis formula intervention. More importantly, our results showed that GCis pretreatment had no significant effect on the mRNA expression of IL-1ß, IL-6, and TNF-α in the brain. These results indicated that the GCis formula could enhance immunity after ICH. The intestinal barrier function was further investigated in the present study, considering the origin of the source of infection. Our results showed that the mRNA expressions of intestinal ZO-1, SIgA, and MUC2 were significantly increased, villi structure was intact, inflammatory cell infiltration was reduced, and goblet cell number was increased after GCis formula treatment. These results suggest that the GCis formula can enhance the intestinal mucosal immune barrier. This study provides a herb formula (GCis) that could enhance peripheral immunity and intestinal mucosal immune barrier to prevent pulmonary infection induced by ICH. It would be beneficial in the prevention of severe clinical infections.

11.
Oncogene ; 41(23): 3239-3250, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35508542

RESUMO

Although enzalutamide improves the overall survival of patients with metastatic prostate cancers, enzalutamide resistance (ENZR) will be inevitably developed. Emerging evidence support that alternative oncogenic pathways may bypass the androgen receptor (AR) signaling to promote ENZR progression, however, the underpinning mechanisms remain poorly defined. Here, we report that the expression of RuvB like AAA ATPase 1 (RUVBL1) is upregulated in ENZR cells and xenograft models and prostate tumors in patients. Enzalutamide increases RUVBL1 accumulation in the cytoplasm, which in turn enhances the recruitment of CRAF proto-oncogene serine/threonine kinase protein to plexin A1 (PLXNA1) and the subsequent activation of the downstream MAPK pathway. Co-overexpression of RUVBL1 and PLXNA1 defines a subgroup of prostate cancer (PCa) patients with a poor prognosis. Furthermore, pharmacological inhibition of RUVBL1 by CB-6644 suppresses ENZR cell proliferation and xenograft growth and allows re-sensitization of ENZR cells and xenografts to enzalutamide, indicating that RUVBL1 may act to substitute the AR signaling to promote cancer cell survival and ENZR development. Together, these findings may lead to the identification of RUVBL1 as a potential therapeutic target for ENZR tumors.


Assuntos
Neoplasias de Próstata Resistentes à Castração , ATPases Associadas a Diversas Atividades Celulares/genética , Benzamidas , Proteínas de Transporte , Linhagem Celular Tumoral , Proliferação de Células , DNA Helicases/genética , DNA Helicases/metabolismo , Resistencia a Medicamentos Antineoplásicos/genética , Humanos , Masculino , Proteínas do Tecido Nervoso/metabolismo , Nitrilas/uso terapêutico , Feniltioidantoína , Neoplasias de Próstata Resistentes à Castração/patologia , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo , Receptores de Superfície Celular/metabolismo
12.
Environ Pollut ; 307: 119494, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35597485

RESUMO

Although greenhouse vegetable production in China is rapidly changing, consumers are concerned about food quality and safety. Studies have shown that greenhouse soils are highly eutrophicated and potentially contaminated by heavy metals. However, to date, no regional study has assessed whether greenhouse soils differ significantly in their heavy metal and nutrient loads compared to adjacent arable land. Our study was conducted in Shouguang County, a key region of greenhouse vegetable production in China. Soil samples down to soil depths of 3 m were taken from 60 greenhouse vegetable fields of three different ages (5, 10, and 20 years) and from 20 adjacent arable fields to analyze the concentrations of heavy metals, nutrients, and soil physio-chemical parameters. A comparison of greenhouse soils with adjacent arable fields revealed that for greenhouses, (a) micro (heavy metals: Cu, Zn, and Mn) and macronutrients (Nmin, Olsen-P, available K) were significantly higher by a factor of about five, (b) N:P:K ratios were significantly imbalanced towards P and K, and (c) topsoil (0-30 cm) concentrations of the above-mentioned micro- and macronutrients increased with years of vegetable cultivation. In contrast, the soil concentrations of the heavy metals Cr and Pb were lower in greenhouse soils. Heavy metal concentrations did not vary significantly with soil depth, except for the micronutrients Cu and Zn, which were between 1- and 3-fold higher in the topsoil (0-30 cm) than in the subsoil (30-300 cm). The Nemerow pollution index (PN) was 0.37, which was below the recommended environmental threshold value (PN < 1). Structural equation model analysis revealed that soil nutrient concentrations in greenhouse soils are directly related to the input of fertilizers and agrochemicals. Lower values of soil Pb and Cr concentrations in greenhouses were due to the sheltering effect of the greenhouse roof, which protected soils from atmospheric deposition due to emissions from nearby industrial complexes.


Assuntos
Metais Pesados , Poluentes do Solo , China , Monitoramento Ambiental , Chumbo/análise , Metais Pesados/análise , Nutrientes/análise , Medição de Risco , Solo/química , Poluentes do Solo/análise , Verduras/química
13.
Micron ; 159: 103293, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35636161

RESUMO

Protein is an important component of human tissues and cells, which is closely related to various life activities. Therefore, studying the conformation of proteins can not only provide better analytic insight into its interactions with biomacromolecules, but also contribute to the biological and medical research. In recent years, the atomic force microscopy with excellent capacity shines brilliantly in obtaining information of biological samples, especially in the research of proteins. Herein, we highlight the advances and main contributions in the understanding of proteins conformation and its interactions with biomacromolecules by atomic force microscopy imaging technology. In addition, we have found that the relevant information of a single investigated object could be directly measured and obtained by atomic force microscopy, which avoids the indirectness and complexity of data analysis. Therefore, it is reasonable to believe that this work will be conducive to promoting the development of atomic force microscopy in the research field of protein imaging, and promoting its further applications in immunology, medicine and biology.


Assuntos
Proteínas , Humanos , Microscopia de Força Atômica/métodos , Conformação Molecular
14.
Toxics ; 10(5)2022 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-35622672

RESUMO

To fulfill sustainability principles, a three-site field experiment was conducted to screen suitably mixed passivators from lime + biochar (L + C, 9000 kgha-1 with a rate of 1:1) and lime + biochar + sepiolite (L + C + S, 9000 kg ha-1 with a rate of 1:1:1), in Yuecheng (YC), Zhuji (ZJ), and Fuyang (FY), where there are typical contaminated soils, in South China. Treated with passivators in soil, DTPA-extractable Cd, Crand Pb in soil were decreased by 9.87-26.3%, 37.2-67.5%, and 19.0-54.2%, respectively; Cd, Cr, and Pb in rice were decreased by 85.9-91.5%, 40.0-76.5%, and 16.4-45.4%, respectively; and these were followed by slightly higher efficacy of L + C + S than L + C. The differences between L + C and L + C + S mainly lie in soil microbial communities, enzymes, and fertility. In YC, treatment with L + C + S increased microbial carbon and activities of urease (EC3.5.1.5) and phosphatase (EC3.1.3.1) by 21.0%, 85.5%, and 22.3%; while treatment with L + C decreased microbial carbon and activities of phosphatase and sucrose (EC3.2.1.26) by 1.31%, 34.9%, and 43.4%, respectively. Moreover, the treatment of FY soils with L + C + S increased microbial carbon and activities of urease, phosphatase, and sucrase by 35.4%, 41.6%, 27.9%, and 7.37%; and L + C treatment only increased the microbial carbon and the activity of phosphatase by 3.14% and 30.3%, respectively. Furthermore, the organic matter and available nitrogen were also increased by 8.8-19.0% and 7.4-14.6% with L + C + S treatments, respectively. These suggested that the combination of L + C + S stimulated the growth of soil microbial communities and increased the activity of soil enzymes. Therefore, the L + C + S strategy can be a practical and effective measure for safe rice production as it was more suitable for the remediation of heavy metals in our experimental sites.

15.
Biology (Basel) ; 11(5)2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35625468

RESUMO

The key to new drug discovery and development is first and foremost the search for molecular targets of drugs, thus advancing drug discovery and drug repositioning. However, traditional drug-target interactions (DTIs) is a costly, lengthy, high-risk, and low-success-rate system project. Therefore, more and more pharmaceutical companies are trying to use computational technologies to screen existing drug molecules and mine new drugs, leading to accelerating new drug development. In the current study, we designed a deep learning computational model MSPEDTI based on Molecular Structure and Protein Evolutionary to predict the potential DTIs. The model first fuses protein evolutionary information and drug structure information, then a deep learning convolutional neural network (CNN) to mine its hidden features, and finally accurately predicts the associated DTIs by extreme learning machine (ELM). In cross-validation experiments, MSPEDTI achieved 94.19%, 90.95%, 87.95%, and 86.11% prediction accuracy in the gold-standard datasets enzymes, ion channels, G-protein-coupled receptors (GPCRs), and nuclear receptors, respectively. MSPEDTI showed its competitive ability in ablation experiments and comparison with previous excellent methods. Additionally, 7 of 10 potential DTIs predicted by MSPEDTI were substantiated by the classical database. These excellent outcomes demonstrate the ability of MSPEDTI to provide reliable drug candidate targets and strongly facilitate the development of drug repositioning and drug development.

16.
ACS Appl Mater Interfaces ; 14(18): 21509-21520, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35500100

RESUMO

Flexible electronics have aroused great interest over the past few years due to their unique advantages of being wearable and lightweight. Introducing the self-healing function into wearable electronics will contribute to the practical applications of wearable electronics by prolonging the devices' lifetime. In this study, a flexible essential oil (EO)-loaded mesoporous silica (EO@AMS)/polyacrylate hybrid hydrogel with superb self-healing and antibacterial properties was prepared. The prepared hybrid hydrogel was found to have excellent piezoresistive sensing performance, which could be particularly suitable for human vital activity monitoring. Benefiting from the strong ionic bonding and multiple hydrogen bonds between polyacrylate and EO@AMS, the hybrid hydrogel could repair its damaged areas with restored sensing and mechanical properties, which suggested excellent self-healing ability. In addition, this hybrid hydrogel, when applied in wearable devices, was found to have high antibacterial ability owing to the slow release of the lemon EO from AMS to kill bacteria. This promising self-healing and antibacterial hybrid hydrogel shows a promising application in wearable electronics for posture monitoring, human-computer interaction, and artificial intelligence.


Assuntos
Óleos Voláteis , Dispositivos Eletrônicos Vestíveis , Antibacterianos/farmacologia , Inteligência Artificial , Condutividade Elétrica , Humanos , Hidrogéis/química , Dióxido de Silício
17.
Int Immunol ; 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35561666

RESUMO

Emerging evidence indicates that hypoxia and immunity play important roles in tumorigenesis and development. However, the hypoxia-immune-related prognostic risk model has not been established in cervical cancer (CC). We aimed to construct a hypoxia-immune-based prognostic risk model with potential application in CC patient prognosis and predicting response to targeted therapy. The RNA-seq data and corresponding clinical information were retrieved from The Cancer Genome Atlas (TCGA) database. Hypoxia and immune status of CC patients were evaluated using the Consensus Clustering method and single sample gene set enrichment analysis (ssGSEA), respectively. The univariate Cox regression, least absolute shrinkage and selection operator (LASSO) and multivariate Cox regression were applied to establish the prognostic risk model of CC. The chemotherapy response for six chemotherapeutic agents of each CC patient was calculated according to the Genomics of Drug Sensitivity in Cancer (GDSC). And the Connectivity Map (CMap) database was performed to screen candidate small molecule drugs. In this study, we identified 7 gene signatures (P4HA2, MSMO1, EGLN1, ZNF316, IKZF3, ISCU, MYO1B) with prognostic values. And the survival time of patients with low-risk was significantly longer than those with high-risk. Meanwhile, CC patients in the high-risk group yielded higher sensitivity to five chemotherapeutic agents. And we listed ten candidate small-molecules drugs that exhibited a high correlation with the prognosis of cervical cancer. Thus, the prognostic model can accurately predict the prognosis of patients with CC and may be helpful for the development of new hypoxia-immune prognostic markers and therapeutic strategies for CC.

18.
Carbohydr Res ; 517: 108577, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35569241

RESUMO

The O-antigen is an important virulence factor involved in the survival, virulence and invasion of bacteria. The bacterial serological types are highly dependent on these surface-exposed and structurally unique O-antigen structures. In this work, the structure of O-antigen from an Escherichia coli strain 2017LL031 was elucidated as a hexasaccharide repeating unit: →3)-[ß-D-Glcp-(1 â†’ 2)]-α-L-Rhap-(1 â†’ 3)-[α-D-Quip-(1 â†’ 3)-α-D-GlcpA-(1 â†’ 2)]-ß-L-Fucp-(1 â†’ 3)-ß-D-GlcpNAc-(1→, which is completely different from all known E. coli serogroups. The O-antigen gene cluster (O-AGC) of 2017LL031 was also analyzed and correlates well to its O-Ag. Moreover, the O-AGC of 2017LL031 was deleted and its role in O-Ag biosynthesis was confirmed experimentally. Taken together, our results present that a novel E.coli serotype 2017LL031 is identified.


Assuntos
Escherichia coli , Antígenos O , Escherichia coli/química , Escherichia coli/genética , Família Multigênica , Antígenos O/química , Antígenos O/genética , Sorogrupo
19.
Sci Total Environ ; 838(Pt 1): 155878, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35580678

RESUMO

To explore the spatial and temporal distributions of dissolved organic matter (DOM) affected by Ulva prolifera bloom and the regulation of DOM released by the addition of nitrate (NO3-N) nutrients, the variation in DOM was analyzed by three cruises in the coastal waters of the southern Yellow Sea (SYS) off Qingdao during a 2018 U. prolifera bloom. A mesocosm experiment was conducted during the decay of U. prolifera. The U. prolifera bloom resulted in higher concentrations of the bulk DOM except for total dissolved amino acids (TDAA), compared with levels recorded during non-bloom periods. Three different fluorescent substances of chromophoric dissolved organic matter (CDOM) were identified, which were also affected by the bloom. Concentrations of total dissolved carbohydrates (TCHO), monosaccharides (MCHO), and polysaccharides (PCHO) peaked during the late bloom, with average levels of 49.78 ± 9.73, 14.23 ± 3.32, and 35.55 ± 9.19 µmol C L-1, respectively, accompanied by an increase of DOC, indicating release of DOM with U. prolifera decay. In contrast, the concentrations of CDOM and TDAA declined during the late bloom, with averages of 0.10 ± 0.10 m-1 and 1.16 ± 0.82 µmol L-1, suggesting that the yields of CDOM and TDAA were regulated by the U. prolifera bloom and other mechanism. These findings were confirmed by the mesocosm experiment. DOM production was obviously improved by NO3-N addition except for TDAA and CDOM. The release of protein-like CDOM was primarily regulated by NO3-N nutrients, however, it was not obvious with humic-like CDOM. Our findings provide evidence supporting the role of U. prolifera blooms in the coastal DOM pool and its significant effect on the marine carbon cycle.


Assuntos
Ulva , 2,5-Dimetoxi-4-Metilanfetamina/metabolismo , China , Eutrofização , Nutrientes , Compostos Orgânicos/metabolismo , Ulva/metabolismo
20.
Sci Total Environ ; 838(Pt 2): 156055, 2022 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-35598674

RESUMO

Harmful algal blooms (HAB) are a serious problem worldwide. Allelochemicals from natural plants were recently thought to be promising anti-algaecide in controlling harmful algae. However, the programmed cell death (PCD) process of algae under allelopathic pressure induced by 5,4'-dihydroxyflavone (5,4'-DHF) was poorly understood. In this study, two common and worldwide distributed microalgae, Microcystis aeruginosa and Phaeocystis globosa were selected as target algae, and the PCD processes induced by 5,4'-DHF were cross-compared between the two species. Both algae species were inhibited significantly by 5,4'-DHF with the relative sensitivity of 0.11. To uncover the PCD progress systematically, signals for PCD triggering, antioxidant enzyme activity, photosynthetic ability variation, caspase-like activities and typical indicators were investigated. In both species, typical indicators of PCD - phosphatidylserine externalization and chromatin condensation - were detected. The intracellular reactive oxygen species (ROS), nitric oxide (NO) and H2O2 were the potential signal molecules to stimulate PCD, and caspase-like activities were activated with an elevation of cytochrome c indicating the initiation of PCD in both species. However, P. globosa responded to 5,4'-DHF immediately after 3 h with the elevation of ROS and not in M. aeruginosa. Antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT) in M. aeruginosa and P. globosa also showed different patterns on day 3. Specifically, SOD activity in M. aeruginosa increased significantly while it decreased significantly in P. globosa, CAT activity in M. aeruginosa decreased significantly while it increased significantly in P. globosa (p < 0.05). Malondialdehyde (MDA) content in P. globosa increased significantly (p < 0.001) while it showed no variation in M. aeruginosa. Overall, this study is one of the earliest studies to explore the inhibition and action mechanism of plant derived flavonoids on harmful algae from the perspective of PCD, and provide new insights into the antialgal mechanism of allelochemicals.


Assuntos
Haptófitas , Microcystis , Antioxidantes/metabolismo , Apoptose , Caspases , Água Doce , Haptófitas/metabolismo , Peróxido de Hidrogênio , Microcystis/metabolismo , Feromônios , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo
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