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1.
Brain Behav ; : e01639, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32415731

RESUMO

OBJECTIVE: This study aimed to investigate the characteristic of brain structural connections in glioma patients and further evaluate the relationship between changes in the white matter tracts and cognitive decline. METHODS: This retrospective study included a total of 35 subjects with glioma and 14 demographically matched healthy controls, who underwent diffusion tensor imaging scans and formal neuropsychological assessment tests. Fractional anisotropy (FA) values of white matter tracts were derived from atlas-based analysis to compare group differences. Furthermore, subgroup-level analysis was performed to differentiate the effects of tumor location on white matter tracts. Partial correlation analysis was used to examine the associations between neurocognitive assessments and the integrity of tracts. Region of interest-based network analysis was performed to validate the alteration of structural brain network in subjects with glioma. RESULTS: Compared with controls, subjects with glioma exhibited reduced FA values in the right uncinate fasciculus. Besides, subjects with glioma exhibited worse performance in several cognitive assessments. Partial correlation analysis indicated that the FA value in the right superior longitudinal fasciculus temporal part was significantly positively correlated with scores of visual-spatial abilities in subjects with glioma in the right temporal lobe (r = .932, p = .002). Region of interest-based network analysis revealed that subjects with glioma exhibited reduced FA, fiber length (FL), and fiber number (FN) between specific brain regions compared with controls. CONCLUSION: The present study demonstrated the reduced integrity of white matter tracts and altered structural connectivity in brain networks in patients with glioma. Notably, white matter tracts in the right hemisphere might be vulnerable to the effects of a frontal or temporal lesion and might be associated with deficient cognitive function.

2.
Ultrasonics ; 107: 106107, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32417694

RESUMO

The application of ultrasonic vibration is an effective method to overcome the processing problems in micro/meso-forming. Previously it was observed that ultrasonic vibration could reduce flowing stress in the forming process, called ultrasonic volume effect. The volume effect contains multi-mechanisms such as stress superposition leading to apparent average stress reduction, acoustic softening and ultrasonic impact leading to real stress reductin. However, the evolutional characteristics and the mechanism of acoustic softening on material deformation is still not clear. And in most previous studies only the average stress but not the oscillatory stress was measured due to the convenience of dynamic force sensing system, which confused the different ultrasonic volume effects, acoustic softening, stress superposition and ultrasonic impact. The purpose of this study is to investigate the effects of acoustic softening on micro/meso-compression behavior and microstructure evolution. An ultrasonic-assisted compression test system with dynamic force sensing technology was developed. And a series of ultrasonic-assisted micro/meso-compression tests at different amplitudes were carried out on pure copper C1100O combining the microstructure analysis by EBSD technique. By analyzing the waveform of the oscillatory stress in the process, acoustic softening was successfully separated from the stress superposition and it was found that the deformation strain plays an important role on the effect of acoustic softening. The stress reduction by acoustic softening increases with the flowing strain or ultrasonic amplitude increasing. Besides, there is an evolutionary transition of acoustic softening ratio between small strain and large strain. When acoustic softening occurs, the low-angle grain boundaries distribute randomly in grains, compared to the piled distribution without ultrasonic assistance, implying motions of the low-angle grain boundaries or dislocation is improved by acoustic softening, resulting in the real stress reduction. In addition, with small deformation strain, the elongated grain becomes equiaxed and dislocation density is significantly reduced, which may be the result of the increased dislocation annihilation due to ultrasonic-induced dynamic recovery. However, with the deformation strain increasing to some extent, acoustic hardening gradually becomes significant, leading to much less effectiveness of acoustic softening on dislocation density reduction. The findings of this study provide an instructive understanding of the underlying mechanisms of acoustic softening in ultrasonic-assisted micro/meso-forming.

3.
Pediatr Infect Dis J ; 2020 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-32404786

RESUMO

BACKGROUND: To describe the characteristics of clinical manifestations of children with 2019 novel coronavirus (2019-nCoV) infection in Chongqing. METHODS: All 25 children with laboratory-confirmed 2019-nCoV infection by real-time reverse transcription-PCR (RNA-PCR) were admitted from the 4 designated treatment hospitals of 2019-nCoV in Chongqing from January 19 to March 12, 2020. Clinical data and epidemiological history of these patients were retrospectively collected and analyzed. RESULTS: The diagnosis was confirmed through RNA-PCR testing. Among the 25 cases, 14 were males and 11 were females. The median age was 11.0 (6.3-14.5) years (range 0.6-17.0 years). All children were related to a family cluster outbreak, and 7 children (28%) with a travel or residence history in Hubei Province. These patients could be categorized into different clinical types, including 8 (32%) asymptomatic, 4 (16%) very mild cases and 13 (52%) common cases. No severe or critical cases were identified. The most common symptoms were cough (13 cases, 52%) and fever (6 cases, 24%). The duration time of clinical symptoms was 13.0 (8.0-25.0) days. In the 25 cases, on admission, 21 cases (84%) had normal white blood cell counts, while only 2 cases (8%) more than 10 × 10/L and 2 cases (8%) less than 4 × 10/L, respectively; 22 cases(88%) had normal CD4+ T lymphocyte counts, while in the remaining 3 cases(8%) this increased mildly; 23 cases had normal CD8+ T lymphocyte counts, while in the remaining 2 cases (8%) CD8+ T lymphocyte counts were mildly increased as well. All Lymphocyte counts were normal. There were no statistical differences of lab results between the groups of asymptomatic cases, mild cases and common cases. There were only 13 cases with abnormal CT imaging, most of which were located in the subpleural area of the bottom of the lung. All patients were treated with interferon, 6 cases combined with Ribavirin, and 12 cases combined with lopinavir or ritonavir. The days from onset to RNA turning negative was 15.20 ± 6.54 days. There was no significant difference of RNA turning negative between the groups of interferon, interferon plus ribavirin and interferon plus lopinavir or ritonavir treatment. All the cases recovered and were discharged from hospital. CONCLUSIONS: The morbidity of 2019-nCoV infection in children is lower than in adults and the clinical manifestations and inflammatory biomarkers in children are nonspecific and milder than that in adults. RNA-PCR test is still the most reliable diagnostic method, especially for asymptomatic patients.

4.
Kaohsiung J Med Sci ; 2020 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-32374939

RESUMO

Cisplatin, as one of the most effective chemotherapeutic agents, its clinical use is limited by serious side effect of nephrotoxicity. Cisplatin-induced nephrotoxicity is closely related to apoptosis induction and activation of caspase. The present study aimed to explore the potential protective effect of ginsenoside Rk1 (Rk1), a rare ginsenoside generated during steaming ginseng, on cisplatin-induced nephrotoxicity and the underlying mechanisms in human embryonic kidney 293 (HEK-293) cells. Our results showed that the reduced cell viability induced by cisplatin could significantly recover by Rk1. Furthermore, glutathione (GSH) as an oxidative index, was elevated and the lipid peroxidation product malondialdehyde (MDA) was significantly decreased after Rk1 treatment compared to the cisplatin group. Additionally, Rk1 can also decrease the ROS fluorescence expression and increase the protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) compared to the cisplatin group, which suggested a suppression of oxidative response. More importantly, the cisplatin-induced elevated protein levels of Bax, cleaved caspase-3, cleaved caspase-9, and decreased protein level of Bcl-2 were reversed after treatment with Rk1. Our results elucidated the possible protective mechanism of Rk1 for the first time, which may involve in its anti-oxidation and anti-apoptosis effects.

5.
Acad Radiol ; 2020 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-32417035

RESUMO

RATIONALE AND OBJECTIVE: To investigate the performance of multi-parametric magnetic resonance imaging (MRI) for glioma grading. MATERIALS AND METHODS: Seventy consecutive patients with histopathologically confirmed glioma were retrospectively evaluated by conventional MRI, dynamic susceptibility-weighted contrast-enhanced, multiple diffusion-weighted imaging signal models including mono-exponential, bi-exponential, stretched exponential, and diffusion kurtosis imaging. One-way analysis of variance and independent-samples t test were used to compare the MR parameter values between low and high grades as well as among all grades of glioma. Receiver operating characteristic analysis, Spearman's correlation analysis, and binary logistic regression analysis were used to assess their diagnostic performance. RESULTS: The diagnostic performance (the optimal thresholds, area under the receiver operating characteristic curve, sensitivity, and specificity) was achieved with normalized relative cerebral blood flow (rCBV) (2.240 ml/100 g, 0.844, 87.8%, and 75.9%, respectively), mean kurtosis (MK) (0.471, 0.873, 92.7%, and 79.3%), and water molecular diffusion heterogeneity index (α) (1.064, 0.847, 79.3% and 78.0%) for glioma grading. There were positive correlations between rCBV and MK and the tumor grades and negative correlations between α and the tumor grades (p < 0.01). The parameter of α yielded a diagnostic accuracy of 85.3%, the combination of MK and α yielded a diagnostic accuracy of 89.7%, while the combination of rCBV, MK, and α were more accurate (94.2%) in predicting tumor grade. CONCLUSION: The most accurate parameters were rCBV, MK, and α in dynamic susceptibility-weighted contrast, diffusion kurtosis imaging, and Multi-b diffusion-weighted imaging for glioma grading, respectively. Multiparametric MRI can increase the accuracy of glioma grading.

6.
Nan Fang Yi Ke Da Xue Xue Bao ; 40(2): 279-286, 2020 Feb 29.
Artigo em Chinês | MEDLINE | ID: mdl-32376541

RESUMO

Extracellular vesicles (EVs), including exosomes and microvesicles, are lipid bilayer-enclosed nanovesicles secreted by cells. These EVs are important mediators of intercellular communication by serving as vehicles for transfer of proteins, mRNA, miRNA and lipids between cells. Various visualization methods have been established to explore the characteristics of EVs and their role in physiological and pathological processes. The nanoscale size and high heterogeneity of EVs hamper the identification of their biological characteristics and functions. This review presents a comprehensive overview of EV imaging methods in light of the origin, separation and dynamic tracking of EVs, and the advantages and disadvantages of different imaging strategies are discussed. We believe that studies at the levels of single vesicles and single cells will become the frontier of future researches of EVs.

7.
Sensors (Basel) ; 20(9)2020 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-32370109

RESUMO

The piezoelectric actuator is indispensable for driving the micro-manipulator. In this paper, a simplified interval type-2 (IT2) fuzzy system is proposed for hysteresis modelling and feedforward control of a piezoelectric actuator. The partial derivative of the output of IT2 fuzzy system with respect to the modelling parameters can be analytically computed with the antecedent part of IT2 fuzzy rule specifically designed. In the experiments, gradient based optimization was used to identify the IT2 fuzzy hysteresis model. Results showed that the maximum error of model identification is 0.42% with only 3 developed IT2 fuzzy rules. Moreover, the model validation was conducted to demonstrate the generalization performance of the identified model. Based on the analytic inverse of the developed model, feedforward control experiment for tracking sinusoidal trajectory of 20 Hz was carried out. As a result, the hysteresis effect of the piezoelectric actuator was reduced with the maximum tracking error being 4.6%. Experimental results indicated an improved performance of the proposed IT2 fuzzy system for hysteresis modelling and feedforward control of the piezoelectric actuator.

8.
Phys Chem Chem Phys ; 2020 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-32374336

RESUMO

We study the interfacial structure and dynamics of a polymer nanocomposite (PNC) composed of octaaminophenyl polyhedral oligomeric silsesquioxane (OAPS) and poly(2-vinylpyridine) (P2VP) by performing full atomistic molecular dynamics simulations. There are eight aminophenyl groups grafted on the surface of the OAPS particle and the particle has a size comparable to the Kuhn segment of P2VP. These aminophenyl groups can form hydrogen bonds (HBs) with pyridine rings from surrounding P2VP chains. We found that OAPS can form ∼2 HBs on average with surrounding polymer chains. The effect of the HBs is investigated in detail by either switching on or off these HBs in our simulation. By analyzing the interfacial static packing structure and dynamic properties, we demonstrate that the system has an ∼1 nm interface width, similar to the OAPS particle size. We also found that HBs can prevent the further penetration of polymers into the inner zone (grafting layer) of the OAPS, and therefore keep the P2VP chains in the outer layer (>1 nm), remaining bulk-like, which is well consistent with experimental results. In addition, we found that NP diffusion is coupled to the absorbed polymer chains, which also dramatically slows down the diffusion of polymer segments in return. The core-shell model in which the NP and absorbed polymers diffuse as a single object is validated here at the full atomistic level. These results provide atomistic insights into the unique structure and dynamics in the small attractive NP-polymer interfacial region. We hope these results will be helpful for the understanding of peculiar phenomena in attractive polymer nanocomposites containing small NPs.

9.
Small ; : e2001098, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32383359

RESUMO

The high-temperature molten-salt method is an important inorganic synthetic route to a wide variety of morphological phenotypes. However, its utility is limited by the fact that it is typically incapable of producing ultrathin (<5 nm diameter) nanowires, which have a crucial role in novel nanotechnology applications. Herein, a rapid molten salt-based synthesis of sub-5-nm-sized nanowires of hexagonal tungsten oxide (h-WO3 ) that is critically dependent on a substantial proportion of molybdenum (Mo) dopant is described. This dopant-driven morphological transition in tungsten oxide (WO3 ) may be attributable to the collapse of layered structure, followed by nanocluster aggregation, coalescence, and recrystallization to form ultrathin nanowires. Interestingly, due to the structural properties of h-WO3 , the thus-formed ultrathin nanowires are demonstrated to be excellent photocatalysts for the production of ammonia (NH3 ) from nitrogen (N2 ) and water. The ultrathin nanowires exhibit a high photocatalytic NH3 -production activity with a rate of 370 µmol g-1 h-1 and an apparent quantum efficiency of 0.84% at 420 nm, which is more than twice that obtained from the best-performing Mo-doped W18 O49 nanowire catalysts. It is envisaged that the dopant replacement-driven synthetic protocol will allow for rapid access to a series of ultrathin nanostructures with intriguing properties and increase potential applications.

10.
Biomed Res Int ; 2020: 2471915, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32420331

RESUMO

Tobacco exposure is one of the major risks for the initiation and progress of lung cancer. The exact corresponding mechanisms, however, are mainly unknown. Recently, a growing body of evidence has been collected supporting the involvement of DNA methylation in the regulation of gene expression in cancer cells. The identification of tobacco-related signature methylation probes and the analysis of their regulatory networks at different molecular levels may be of a great help for understanding tobacco-related tumorigenesis. Three independent lung adenocarcinoma (LUAD) datasets were used to train and validate the tobacco exposure pattern classification model. A deep selecting method was proposed and used to identify methylation signature probes from hundreds of thousands of the whole epigenome probes. Then, BIMC (biweight midcorrelation coefficient) algorithm, SRC (Spearman's rank correlation) analysis, and shortest path tracing method were explored to identify associated genes at gene regulation level and protein-protein interaction level, respectively. Afterwards, the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis and GO (Gene Ontology) enrichment analysis were used to analyze their molecular functions and associated pathways. 105 probes were identified as tobacco-related DNA methylation signatures. They belong to 95 genes which are involved in hsa04512, hsa04151, and other important pathways. At gene regulation level, 33 genes are uncovered to be highly related to signature probes by both BIMC and SRC methods. Among them, FARSB and other eight genes were uncovered as Hub genes in the gene regulatory network. Meanwhile, the PPI network about these 33 genes showed that MAGOH, FYN, and other five genes were the most connected core genes among them. These analysis results may provide clues for a clear biological interpretation in the molecular mechanism of tumorigenesis. Moreover, the identified signature probes may serve as potential drug targets for the precision medicine of LUAD.

11.
Bioinformatics ; 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32437538

RESUMO

MOTIVATION: At present, a fundamental challenge in single-cell RNA-sequencing data analysis is functional interpretation and annotation of cell clusters. Biological pathways in distinct cell types have different activation patterns, which facilitates the understanding of cell functions using single-cell transcriptomics. However, no effective web tool has been implemented for single-cell transcriptome data analysis based on prior biological pathway knowledge. RESULTS: Here, we present scTPA, a web-based platform for pathway-based analysis of single-cell RNA-seq data in human and mouse. scTPA incorporates four widely-used gene set enrichment methods to estimate the pathway activation scores of single cells based on a collection of available biological pathways with different functional and taxonomic classifications. The clustering analysis and cell-type-specific activation pathway identification were provided for the functional interpretation of cell types from a pathway-oriented perspective. An intuitive interface allows users to conveniently visualize and download single-cell pathway signatures. Overall, scTPA is a comprehensive tool for the identification of pathway activation signatures for the analysis of single cell heterogeneity. AVAILABILITY: http://sctpa.bio-data.cn/sctpa. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

12.
Nanotechnology ; 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32438348

RESUMO

Transition metal selenides (TMS), on account of their relatively high theoretical capacity, unique electrical properties, easy compositing and low cost, are considered to be a candidate anode for potassium-ion batteries (KIBs). However, the cycling stability of TMS is unsatisfactory owing to the large K ion intercalation/deintercalation. Herein, CoSe2/N-doped carbon porous frame (CoSe2@NC) is successfully synthesized through a simple mixing and sintering approach and displays excellent potassium storage performance. Plentiful C-N bonds in the precursor can induce the formation of homogeneous N-doped carbon matrix and C-N-Co bonds, thus endowing robust structure and high electronic conductivity for superior cycling stability. Therefore, the unique porous nanoframe suppresses volume expansion and provides more diffusion paths for K ions. After 1000 cycles at 50 mA g-1, a high capacity of 311.3 mAh g-1 is acquired. When the current density increases to 500mA g-1, the CoSe2@NC can still maintain a capacity of 184.5 mAh g-1 after 1000 cycles. The high performance, easy compositing and low cost of the CoSe2@NC makes it a favorable material for KIBs application.

13.
Nat Immunol ; 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32367036

RESUMO

Cytosolic sensing of pathogens and damage by myeloid and barrier epithelial cells assembles large complexes called inflammasomes, which activate inflammatory caspases to process cytokines (IL-1ß) and gasdermin D (GSDMD). Cleaved GSDMD forms membrane pores, leading to cytokine release and inflammatory cell death (pyroptosis). Inhibiting GSDMD is an attractive strategy to curb inflammation. Here we identify disulfiram, a drug for treating alcohol addiction, as an inhibitor of pore formation by GSDMD but not other members of the GSDM family. Disulfiram blocks pyroptosis and cytokine release in cells and lipopolysaccharide-induced septic death in mice. At nanomolar concentration, disulfiram covalently modifies human/mouse Cys191/Cys192 in GSDMD to block pore formation. Disulfiram still allows IL-1ß and GSDMD processing, but abrogates pore formation, thereby preventing IL-1ß release and pyroptosis. The role of disulfiram in inhibiting GSDMD provides new therapeutic indications for repurposing this safe drug to counteract inflammation, which contributes to many human diseases.

14.
Brief Bioinform ; 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32436937

RESUMO

X-ray crystallography is the major approach for determining atomic-level protein structures. Because not all proteins can be easily crystallized, accurate prediction of protein crystallization propensity provides critical help in guiding experimental design and improving the success rate of X-ray crystallography experiments. This study has developed a new machine-learning-based pipeline that uses a newly developed deep-cascade forest (DCF) model with multiple types of sequence-based features to predict protein crystallization propensity. Based on the developed pipeline, two new protein crystallization propensity predictors, denoted as DCFCrystal and MDCFCrystal, have been implemented. DCFCrystal is a multistage predictor that can estimate the success propensities of the three individual steps (production of protein material, purification and production of crystals) in the protein crystallization process. MDCFCrystal is a single-stage predictor that aims to estimate the probability that a protein will pass through the entire crystallization process. Moreover, DCFCrystal is designed for general proteins, whereas MDCFCrystal is specially designed for membrane proteins, which are notoriously difficult to crystalize. DCFCrystal and MDCFCrystal were separately tested on two benchmark datasets consisting of 12 289 and 950 proteins, respectively, with known crystallization results from various experimental records. The experimental results demonstrated that DCFCrystal and MDCFCrystal increased the value of Matthew's correlation coefficient by 199.7% and 77.8%, respectively, compared to the best of other state-of-the-art protein crystallization propensity predictors. Detailed analyses show that the major advantages of DCFCrystal and MDCFCrystal lie in the efficiency of the DCF model and the sensitivity of the sequence-based features used, especially the newly designed pseudo-predicted hybrid solvent accessibility (PsePHSA) feature, which improves crystallization recognition by incorporating sequence-order information with solvent accessibility of residues. Meanwhile, the new crystal-dataset constructions help to train the models with more comprehensive crystallization knowledge.

15.
Phys Chem Chem Phys ; 22(19): 11045-11052, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32369059

RESUMO

The search for 2D ferromagnets with versatile magneto-electronic properties is becoming more active due to their potential applications in spintronic devices. To screen out the optimal compositions, we have explored a series of two-dimensional M3C12X12 (M = 5d transition metals, and X = S, NH, and O) metal-organic frameworks with Kagome lattice patterns through first-principles calculations. By varying the metal center and ligand functional radicals, both the electronic and spin-related properties can be easily tuned to meet the requirements for multifunctional applications in spintronic devices. Among them, Re3C12N12H12 is identified to be a ferromagnetic bipolar magnetic semiconductor with the highest Curie temperature (TC > 330 K). Re3C12O12 is found to be an ideal half-metal with a spin gap of 0.97 eV, which is beneficial for use as a spin-filter. Meanwhile, both Re3C12N12H12 and Re3C12O12 exhibit considerable out-of-plane magnetic anisotropy energies (>26 meV per atom), which benefit the spintronic applications. The theoretical results not only show that the 2D organometallic Kagome lattice is a good platform for designing spintronic materials, but also provides a feasible way to realize robust spin manipulation.

16.
J Phys Chem Lett ; 2020 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-32428411

RESUMO

Interest in photovoltaic devices based on layered perovskites is motivated by their tunable optoelectronic properties and stabilities in humid conditions. In these systems, quantum wells with different sizes are organized to direct energy and charge transport between electrodes; however, these relaxation mechanisms are difficult to distinguish based on conventional transient absorption techniques. Here, two-dimensional "action spectroscopies" are employed to separately target processes that lead to the production of photocurrent and energy loss due to fluorescence emission. These measurements show that energy transfer between quantum wells dominates the sub-ns time scale, whereas electron transfer occurs at later times. Overall, this study suggests that while the intense exciton transitions promote light harvesting, much of the absorbed energy is lost by way of spontaneous emission. This limitation may be overcome with alternate layered perovskite systems that combine smaller exciton binding energies with large absorbance cross sections in the visible spectral range.

17.
J Xray Sci Technol ; 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32333573

RESUMO

OBJECTIVE: To evaluate the diagnostic value of ultrasonography and mammography for plasma cell mastitis. METHODS: The ultrasonographic and mammographic images of 111 women with histopathologically confirmed plasma cell mastitis were retrospectively analyzed. The diagnostic accuracy of the two imaging methods was compared. RESULTS: Ultrasonography identified 91 out of 111 (82.0%) patients with plasma cell mastitis, while the other twenty (18.0%) patients were misdiagnosed. Mammography identified 83 (74.8%) out of 111 patients with plasma cell mastitis. Nineteen (17.1%) patients were misdiagnosed. There was no significant difference between the diagnostic accuracy of using two imaging tests (P = 0.127). Mammography clouds identified 11 out of 20 patients who were not diagnosed by ultrasonography. Meanwhile, 19 of the 28 cases misdiagnosed with mammography were correctly identified by ultrasonography. Overall, 91.9% (102/111) of the patients were identified by the combination of ultrasonography and mammography, which yielded statistically significant higher accuracy than using each single test only (P < 0.05). CONCLUSIONS: In diagnosis of plasma cell mastitis, high-frequency ultrasonography and mammography should be combined to improve the diagnostic accuracy.

18.
Photodiagnosis Photodyn Ther ; : 101791, 2020 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-32344196

RESUMO

BACKGROUND: There are 3 main techniques for the removal of epidermoid cysts: traditional wide excision, minimal excision, and punch biopsy excision. For inflamed cysts, the wall is more friable and, therefore, more difficult to remove completely. The classic surgical excision always leads to a long scar or high rate of recurrence. CO2 laser has been proven to result in minimal incision, less bleeding, no suture, and a smaller or no scar. Photodynamic therapy (PDT) has been proposed as an antimicrobial alternative for common and drug-resistant bacteria in nonspecific and multiple sites. It was also shown to be effective in accelerating healing and inhibiting excessive proliferation of hyperplastic scar. Thus, we combined minimally invasive CO2 laser incision with PDT for epidermoid cysts with infection. METHODS: Thirty-three patients had a total of 39 infectious cysts. Two of the patients withdrew due to the high cost after 1 treatment session. After local injection of anesthesia, a hole measuring 2-3 mm was made at the pore in the upper part of the cyst along skin texture by CO2 laser (power 5 W, surgical pattern). The contents of the cyst were extracted through the hole using a curette and compression with gauze. PDT was then performed immediately. A total of 3 PDT sessions were recommended. The overall clinical effects, recurrence rates, cosmetic outcomes, adverse events, and patient satisfaction were assessed. RESULTS: We achieved a 97% success rate in 31 patients with 34 lesions using a combination of minimally invasive CO2 laser incision with PDT. At the 6- to 12-month follow-up, 30 of the patients had excellent cosmetic outcomes and satisfactory therapeutic effect. Pain during the illumination process, which can be relieved by dynamic cold air, was the primary adverse event. CONCLUSION: Our results demonstrate promise for the combination of minimally invasive CO2 laser incision with PDT as a safe and effective therapy for epidermoid cysts with infection. This treatment can inactivate a wide range of microbes including gram-positive and -negative bacteria, without developing drug resistance. Furthermore, it can promote fast wound healing and reduce scar formation.

19.
BMB Rep ; 53(4): 240, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32340662

RESUMO

[Erratum to: BMB Reports 2010; 43(8): 554-560, PMID: 20797318] The authors apologize that due to our neglect when doing the picture layout of Fig. 1A, the wrong Western blot analysis image were pasted for the "ß-actin"group in the middle plot of Fig. 1A. The middle plot of Fig. 1A and its related statistics results (bottom plot in Fig.1A) have been corrected. However, the conclusions of the original article are not affected. The authors would like to apologize for any inconvenience caused.

20.
Med Oncol ; 37(5): 44, 2020 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-32270306

RESUMO

Trillin is a constituent of total Trillium Tschonoskii Maxim (TTM), which is extracted from TTM and displayed anti-tumor effect in many tumor cell lines. However, the anti-tumor mechanism of trillin is still unclear. This study demonstrated that trillin could dramatically inhibit hepatoma carcinoma cell proliferation, induce apoptosis and decrease migration and invasion through suppressing phosphorylated STAT3 translocated to nucleus. Trillin could down-regulate Bcl-2 and Survivin, up-regulate cleaved PRAP, leading to dramatically apoptosis; trillin could also down-regulate MMP1, MMP2, MucI and VEGF, which displayed an inhibition effect on hepatocellular tumor cells invasion and development. The results of this study indicated the potential utility of trillin as a STAT3 inhibitor for the treatment of cancers.

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