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1.
BMC Plant Biol ; 23(1): 7, 2023 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-36600201

RESUMO

BACKGROUND: Heat stress has adverse effects on the growth and reproduction of plants. Zygophyllum xanthoxylum, a typical xerophyte, is a dominant species in the desert where summer temperatures are around 40 °C. However, the mechanism underlying the thermotolerance of Z. xanthoxylum remained unclear. RESULTS: Here, we characterized the acclimation of Z. xanthoxylum to heat using a combination of physiological measurements and transcriptional profiles under treatments at 40 °C and 45 °C, respectively. Strikingly, moderate high temperature (40 °C) led to an increase in photosynthetic capacity and superior plant performance, whereas severe high temperature (45 °C) was accompanied by reduced photosynthetic capacity and inhibited growth. Transcriptome profiling indicated that the differentially expressed genes (DEGs) were related to transcription factor activity, protein folding and photosynthesis under heat conditions. Furthermore, numerous genes encoding heat transcription shock factors (HSFs) and heat shock proteins (HSPs) were significantly up-regulated under heat treatments, which were correlated with thermotolerance of Z. xanthoxylum. Interestingly, the up-regulation of PSI and PSII genes and the down-regulation of chlorophyll catabolism genes likely contribute to improving plant performance of Z. xanthoxylum under moderate high temperature. CONCLUSIONS: We identified key genes associated with of thermotolerance and growth in Z. xanthoxylum, which provide significant insights into the regulatory mechanisms of thermotolerance and growth regulation in Z. xanthoxylum under high temperature conditions.


Assuntos
Termotolerância , Zanthoxylum , Zygophyllum , Termotolerância/genética , Sódio/metabolismo , Zygophyllum/genética , Zygophyllum/metabolismo , Zanthoxylum/genética , Transcriptoma , Perfilação da Expressão Gênica , Resposta ao Choque Térmico/genética , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Temperatura Alta , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas
2.
Eur J Med Genet ; 66(2): 104693, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36587802

RESUMO

Unexplained diarrhea and cholestasis are common clinical phenotypes in newborns, indicating there is only a little common genetic basis for these conditions. However, it has been reported that defects in the UNC45A gene can lead to osteo-oto-hepato-enteric syndrome. However, to date, only 10 patients with this syndrome have been reported in 2 studies; therefore, there is still a lack of analysis regarding the correlation between disease phenotype and genotype. Trio-whole exome sequencing was conducted using DNA samples from a newborn with congenital diarrhea and cholestasis from a Chinese Han family. The UNC45A variants were verified using Sanger sequencing. In addition, we applied a crystal structure model to analyze the potential hazards associated with the variants. The plasmids were constructed in vitro and transfected into human 293T cells for Western blot (WB) analysis. After the mutant protein was fused with the Green Fluorescent Protein label, intracellular localization was observed using laser confocal microscopy. The gene detection results showed that the UNC45A gene of the newborn examined in the present study harbored the compound heterozygous variants p.Arg819Ter, and p.Leu237Pro; this was confirmed via Sanger sequencing. Analysis of the Leu237Pro crystal structure model suggested that this variant may decrease local structural stability and affect protein function. The Western blot and laser confocal microscopy observation results suggested that the Leu237Pro mutation leads to reduced protein expression, while the Arg819Ter mutation completely inhibits the expression of the protein. The compound heterozygous variants of UNC45A (p.Arg819Ter and p.Leu237Pro) may be pathogenic factors of congenital diarrhea and cholestasis in this neonatal patient. Therefore, UNC45A deficiency should be considered when intractable diarrhea and cholestasis occur in newborns.


Assuntos
Síndrome Brânquio-Otorrenal , Colestase , Humanos , Recém-Nascido , Mutação , Síndrome Brânquio-Otorrenal/genética , Chaperonas Moleculares/genética , Diarreia , Peptídeos e Proteínas de Sinalização Intracelular/genética
3.
Environ Pollut ; 319: 121014, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36608727

RESUMO

Zero-valent iron (Fe) is commonly employed as an additive for the mechanochemical destruction (MCD) of organic pollutants. The poly- and perfluoroalkyl substances (e.g., perfluorooctane sulfonate, PFOS) are a class of toxic environmental pollutants that are difficult to effectively degrade due to their thermodynamic and chemical stability. In this study, magnetite (Fe3O4) was applied to improve the milling performance of Fe to PFOS and its promoting mechanisms were emphatically explored. The desulfurization rate was in ahead of the defluorination rate because the C-S bond is less stable than the C-F bonds in PFOS. Fe3O4 had an excellent reinforcement effect on the milling performance of Fe, which was mainly through accelerating the electron transfer as a conductor, reacting with Fe to produce FeO, and facilitating the formation of HO●. During the MCD of PFOS with Fe/Fe3O4 as an additive, HO● played a dominant role in the defluorination process (accounting for >67%). After the elimination of sulfonate group (-SO3-), the produced radical (C7F15CF2●) continued to react through two main pathways: one was the stepwise defluorination after hydrogenation, and the other one was oxidation reaction after alcoholization to yield the corresponding aldehydes and carboxylic acids. The optimum Fe fraction (MFe) was 30%, and air atmosphere was more effective than oxygen and nitrogen conditions. This study helps to comprehensively understand the role of Fe3O4 in defluorination and fills the gap of Fe/Fe3O4 application in the MCD of PFASs.


Assuntos
Ácidos Alcanossulfônicos , Poluentes Ambientais , Fluorcarbonetos , Ferro/química , Óxido Ferroso-Férrico , Ácidos Alcanossulfônicos/química , Fluorcarbonetos/química , Poluentes Ambientais/química
4.
Angew Chem Int Ed Engl ; : e202218227, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36624053

RESUMO

Jump is an important form of motion that enables animals to escape from predators, increase their range of activities, and better adapt to the environment. Inspired by springtails, we describe a light-driven soft jumping robot based on a double-folded liquid crystal elastomer (LCE) ribbon actuator with a monolithic three-leaf panel fold structure. This robot can achieve remarkable jumping height, jumping distance, and maximum take-off velocity, of up to 87 body length (BL), 65 BL, and 930 BL s-1 , respectively, under near-infrared light irradiation. Further, it is possible to control the height, distance, and direction of jump by changing the size and crease angle of the double-folded LCE ribbon actuators. These robots can efficiently jump over obstacles and can jump continuously, even in complex environments. Our simple design strategy improves the performance of jumping actuators and we expect it to have a wide-ranging impact on the strength, continuity, and adaptability of future soft robots.

5.
Int Immunopharmacol ; 115: 109661, 2023 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-36608440

RESUMO

Suppression of the immune microenvironment is an important endogenous contributor to treatment failure in lung cancer. Photodynamic therapy (PDT) is widely used in the treatment of malignant tumors owing to its photo-selectivity and minimal side effects. Some studies have shown the ability of photodynamic action not only to cause photo-cytotoxicity to tumor cells but also to induce immunogenic cell death (ICD). However, the mechanism by which PDT enhances tumor immunogenicity is poorly understood. The present study aimed to explore the immunogenicity effect of PDT on lung cancer and to reveal the underlying mechanism. First, we searched for effective conditions for PDT-induced apoptosis in lung cancer cells. Just as expected, chlorin e6 (Ce6) PDT could enhance the immunogenicity of lung cancer cells alongside the induction of apoptosis, characterized by up-regulation of CRT, HSP90, HMGB1 and MHC-I. Further results showed the generation of ROS by Ce6 PDT under the above conditions, which is an oxidative damaging agent. Simultaneously, PDT induced endoplasmic reticulum (ER) stress in cells, as evidenced by enhanced Tht staining and up-regulated CHOP and GRP78 expression. Moreover, PDT led to DNA damage response (DDR) as well. However, the redox inhibitor NAC abolished the ER stress and DDR caused by PDT. More importantly, NAC also attenuated PDT-induced improvement of immunogenicity in lung cancer. On this basis, the PDT-induced CRT up-regulation was found to be attenuated in response to inhibition of ER stress. In addition, PDT-induced increase in HMGB1 and HSP90 release was blocked by inhibition of DDR. In summary, Ce6 PDT could produce ROS under certain conditions, which leads to ER stress that promotes CRT translocation to the cell membrane, and the resulting DNA damage causes the expression and release of nuclear HMGB1 and HSP90, thereby enhancing the immunogenicity of lung cancer. This current study elucidates the mechanism of PDT in ameliorating the immunogenicity of lung cancer, providing a rationale for PDT in regulating the immune microenvironment for the treatment of malignant tumors.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36602730

RESUMO

The objective of current study is to explore the energy recovery potential of fermentation residues. In this perspective, pyrolysis characteristics, kinetics, and modified biochar derived from pine sawdust after fermentation (FPD) were determined, and comparison was established with pine sawdust (PD). The variation range of comprehensive pyrolysis index (CPI) values of FPD was found 6.51 × 10-7-16.38 × 10-7%2·min-2·°C-3, significantly higher than that of untreated samples determined under the same experimental conditions. The average activation energy of FPD was 367.95 kJ/mol, 389.45 kJ/mol, and 346.55 kJ/mol calculated by Flynn-Wall-Ozawa (FWO) method, Kissinger-Akahira-Sonuse (KAS), and Starink method respectively, and importantly, these values are much higher than those of PD. Additionally, fermentation could enhance the adsorption capacity for methylene blue of biochar from 0.76 mg/g to 1.6 mg/g due to the abundant surface functional groups and three-dimensional internal pore structure. The adsorption pattern of fermented pine wood shifted from chemisorption dominated to the synergetic adsorption of surface functional groups adsorption and intragranular filling. These results show that FPD has favorable pyrolytic properties, and the derived biochar has adsorption properties, which is the basis for designing pyrolysis process and reusing fermentation residues. HIGHLIGHTS: The FPD has higher values of CPI and activation energy than the PD. FPD-derived biochar has higher adsorption capacity than PD-derived biochar. The fermentation improves the pyrolysis performance. The fermentation enhances adsorption capacity due to unique structure of biochar.

7.
Artigo em Inglês | MEDLINE | ID: mdl-36655733

RESUMO

Two-dimensional covalent organic frameworks (2D COFs) have been well employed for electrocatalytic systems because of their structural diversities. However, the atomic utilization efficiency was still under improved, because of the catalytic centers located in the basal layers, which is difficult to access the electrolytes. Herein, we have first demonstrated 1D COFs for 2e- oxygen reduction reaction (ORR). With using different four-connectivity blocks, the prepared 1D COFs display good crystallinity, high surface areas, and excellent chemical stability. The more exposed catalytic sites made the 1D COFs showed higher electrochemically active surface areas, 4.8-fold of that of controlled 2D COF, and thus enable to catalyze ORR with higher H2O2 selectivity of 85.8% and activity with TOF value of 0.051 s-1 at 0.2 V, than those of 2D COF (72.9% and 0.032 s-1). This work paves the way to developing COFs with low dimension for electrocatalysis.

8.
Small ; : e2205291, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36635000

RESUMO

Fabricating polymer electrolyte membranes (PEMs) simultaneously with high ion conductivity and selectivity has always been an ultimate goal in many membrane-integrated systems for energy conversion and storage. Constructing broader ion-conducting channels usually enables high-efficient ion conductivity while often bringing increased crossover of other ions or molecules simultaneously, resulting in decreased selectivity. Here, the ultra-small carbon dots (CDs) with the selective barriers are self-assembled within proton-conducting channels of PEMs through electrostatic interaction to enhance the proton conductivity and selectivity simultaneously. The functional CDs regulate the nanophase separation of PEMs and optimize the hydration proton network enabling higher-efficient proton transport. Meanwhile, the CDs within proton-conducting channels prevent fuel from permeating selectively due to their repelling and spatial hindrance against fuel molecules, resulting in highly enhanced selectivity. Benefiting from the improved conductivity and selectivity, the open-circuit voltage and maximum power density of the direct methanol fuel cell (DMFC) equipped with the hybrid membranes raised by 23% and 93%, respectively. This work brings new insight to optimize polymer membranes for efficient and selective transport of ions or small molecules, solving the trade-off of conductivity and selectivity.

9.
Br J Anaesth ; 130(2): e263-e271, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36503826

RESUMO

BACKGROUND: Frailty is a risk factor for postoperative delirium (POD), and has led to preoperative interventions that have reduced, but not eliminated, the risk. We hypothesised that EEG suppression, another risk factor for POD, mediates some of the frailty risk for POD. METHODS: A prospective cohort study enrolled patients aged 65 yr or older, scheduled for noncardiac surgery under total intravenous anaesthesia. Frailty was assessed using the FRAIL scale. Cumulative duration of EEG suppression, defined as an amplitude between -5 and 5 µV for >0.5 s during anaesthesia, was measured. POD was diagnosed by either confusion assessment method (CAM), CAM-ICU, or medical records. The severity of POD was assessed using the Delirium Rating Scale - Revised-98 (DRS). Mediation analysis was used to estimate the relationships between frailty, EEG suppression, and severity of POD. RESULTS: Among 252 enrolled patients, 51 were robust, 129 were prefrail, and 72 were frail. Patients classified as frail had higher duration of EEG suppression than either the robust (19 vs 0.57 s, P<0.001) or prefrail groups (19 vs 3.22 s, P<0.001). Peak delirium score was higher in the frail group than either the robust (17 vs 15, P<0.001) or prefrail groups (17 vs 16, P=0.007). EEG suppression time mediated 24.2% of the frailty-DRS scores association. CONCLUSION: EEG suppression time mediated a statistically significant portion of the frailty-POD association in older noncardiac surgery patients. Trials directed at reducing EEG suppression time could result in intraoperative interventions to reduce POD in frail patients. CLINICAL TRIAL REGISTRATION: ChiCTR2000041092 (Chinese Clinical Trial Registry).


Assuntos
Delírio , Delírio do Despertar , Fragilidade , Humanos , Idoso , Fragilidade/diagnóstico , Fragilidade/complicações , Estudos Prospectivos , Delírio/etiologia , Análise de Mediação , Fatores de Risco , Eletroencefalografia , Complicações Pós-Operatórias/diagnóstico
10.
Environ Pollut ; 319: 120972, 2023 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-36584856

RESUMO

Chlorinated-halonitromethanes (Cl-HNMs) including chloronitromethane (CNM), dichloronitromethane (DCNM), and trichloronitromethane (TCNM) are nitrogenous disinfection by-products, which have high cytotoxicity and genotoxicity to human. This study aimed to investigate the degradation kinetic modeling and mechanism of Cl-HNMs under monochloramine activated by ultraviolet of 254 nm (UV/NH2Cl) treatment. The first-principle kinetic model of UV/NH2Cl process was developed to simulate Cl-HNMs degradation. Of note, the second-order rate constants of Cl-HNMs reacting with HO• (∼108 M-1 s-1), Cl• (kCl•,CNM or DCNM = âˆ¼1010 M-1 s-1, kCl•,TCNM = âˆ¼102 M-1 s-1), Cl2•- (kCl•,CNM or DCNM = âˆ¼109 M-1 s-1, kCl•,TCNM = âˆ¼101 M-1 s-1), ClO• (∼105-106 M-1 s-1) and CO3•- (∼106-107 M-1 s-1) were obtained by the first-principle kinetic model. Overall, Cl-HNMs degradation under UV/NH2Cl treatment was successfully predicted by the kinetic model under various conditions. It was found that UV (>60%) was dominant in Cl-HNMs degradation, followed by HO• (3.8%-24.5%), reactive chlorine species (RCS, 0.9%-28.8%) and CO3•- (0-26.1%). Among the contributions of RCS, Cl• and Cl2•- were main radicals in the degradation of CNM and DCNM, while ClO• was responsible for the abatement of TCNM. The minimum EE/O values under UV/NH2Cl treatment were approximately 30% lower than those under UV treatment. Finally, the possible degradation pathways were proposed, including hemolytic/heterolytic cleavage of Cl-HNMs by UV irradiation, hydrogen abstraction/electron transfer of CNM and DCNM and adduct reaction of TCNM by free radicals. This study based on the kinetic model is beneficial to predict and control the concentrations of Cl-HNMs under UV/NH2Cl treatment.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Humanos , Poluentes Químicos da Água/análise , Cloro/análise , Cloraminas , Halogênios , Desinfecção , Cinética , Raios Ultravioleta , Oxirredução
11.
Food Chem ; 408: 135166, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36521293

RESUMO

Off-flavors can have significant impacts on the quality of salmonid products. This study investigated the possibility of comprehensive off-flavor profiling considering both olfactory and taste sensory perspectives by combining near-infrared hyperspectral imaging (NIR-HSI) and machine/deep learning. Four feature extraction algorithms were employed for the extraction and interpretation of spectral fingerprint information regarding off-flavor-related compounds. Classification models, including the partial least squares discriminant analysis, least-squares support vector machine, extreme learning machine, and one-dimensional convolutional neural network (1DCNN) were constructed using the full wavelengths and selected spectral features for the identification of off-flavor salmonids. The 1DCNN achieved the highest discrimination accuracy with full and selected wavelengths (i.e., 91.11 and 86.39 %, respectively). Furthermore, the prediction and visualization of off-flavor-related compounds were achieved with acceptable performances (R2 > 0.6) for practical applications. These results indicate the potential of NIR-HSI for the off-flavor profiling of salmonid muscle samples for producers and researchers.


Assuntos
Imageamento Hiperespectral , Espectroscopia de Luz Próxima ao Infravermelho , Espectroscopia de Luz Próxima ao Infravermelho/métodos , Redes Neurais de Computação , Algoritmos , Máquina de Vetores de Suporte , Análise dos Mínimos Quadrados
12.
Food Funct ; 14(1): 74-86, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36504256

RESUMO

In this work, we investigated the ameliorative effects of platycodin D (PD), a major active chemical ingredient isolated from the roots of Platycodon grandiflorum (PG), on high-fat diet (HFD)/streptozotocin (STZ)-induced type 2 diabetes (T2D) mice. PD treatment (2.5 and 5.0 mg kg-1) improved HFD-induced body weight gain. PD administration also decreased the fasting blood glucose (FBG) level and improved glucose and insulin tolerance levels. These data collectively showed that PD could maintain glucose homeostasis. In addition, the diabetic mice with PD treatment also showed fewer pathological changes in liver tissues and improved hepatic functional indexes with respect to the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and recovery of abnormal liver function caused by T2D. Except for these, PD decreased the decomposition of hepatic glycogen. The results from western blot analysis showed that PD treatment might regulate the hepatic gluconeogenesis pathway with the increased phosphorylation/expression of AMPK and decreased expressions of PCK1 and G6Pase. In the aspect of lipid metabolism, PD decreased the whole-body lipid levels, including total cholesterol (TC), triglycerides (TG), and high-density lipoprotein (HDL), and reduced the hepatic fat accumulation induced by T2D through the AMPK/ACC/CPT-1 fatty acid anabolism pathway. In addition, the results of molecular docking showed that PD may have a potential direct effect on AMPK and other key glycolipid metabolism proteins. To summarize, PD modulation of hepatic glycolipid metabolism abnormalities is promising for T2D therapy in the future.


Assuntos
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Hiperglicemia , Animais , Camundongos , Proteínas Quinases Ativadas por AMP/genética , Proteínas Quinases Ativadas por AMP/metabolismo , Glicemia/metabolismo , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Tipo 2/metabolismo , Dieta Hiperlipídica , Glucose/metabolismo , Hiperglicemia/metabolismo , Fígado/metabolismo , Simulação de Acoplamento Molecular , Estreptozocina
13.
Free Radic Biol Med ; 195: 89-102, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36581059

RESUMO

Renal tubular damage plays a key role in the pathogenesis of diabetic kidney disease (DKD), and one of the main pathological process associated with DKD in diabetic mice is the ferroptosis, a novel form of cell death caused by iron-dependent lipid peroxidation. Several researches suggested that empagliflozin may treat renal injury, but its effects on diabetic-related ferroptosis and underlying mechanisms were not fully elucidated. In this study, the influence of empagliflozin on renal injury was evaluated in vivo and in vitro in a mouse model and in high-glucose (HG) or Erastin-stimulated renal HK-2 cell line, respectively. Ferroptosis-related markers were assessed, including GSH, labile iron levels, and ferroptosis regulators by Western blot, qRT-PCR, immunohistochemistry, and immunofluorescence. The level of malondialdehyde (MDA) and the fluorescence intensity of BODIPY probe indicated the level of lipid peroxidation. It was demonstrated that solute carrier family 7, member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) were less expressed in renal biopsy samples from patients affected by DKD than in those from non-diabetic renal disease patients (NDRD), proving the ferroptosis of tubular epithelial cells in case of DKD. Furthermore, empagliflozin markedly decreased the ferroptosis impairment in DKD mice, as well as in HG model of HK-2 cells. Our investigations showed the ability of empagliflozin to suppress ferroptosis was partially countered by AMP-activated protein kinase (AMPK) inhibitor, which led to a reduction of the nuclear translocation of the antioxidant transcription factor NFE2-related factor 2 (NRF2) and downregulation of target genes such as GPX4, ferritin heavy chain 1 (FTH1), and SLC7A11, while AMPK agonists were responsible for the enhancement of the protective effects of empagliflozin. Taken together, our findings showed that empagliflozin may prevent the development of ferroptosis by promoting the AMPK-mediated NRF2 activation pathway, providing important insights for possible novel treatment approaches for DKD.


Assuntos
Diabetes Mellitus Experimental , Nefropatias Diabéticas , Ferroptose , Animais , Camundongos , Nefropatias Diabéticas/tratamento farmacológico , Nefropatias Diabéticas/genética , Proteínas Quinases Ativadas por AMP/genética , Fator 2 Relacionado a NF-E2/genética , Diabetes Mellitus Experimental/tratamento farmacológico
14.
ACS Appl Mater Interfaces ; 15(1): 1704-1717, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36541611

RESUMO

The source-drain electrode with a MoO3 interfacial modification layer (IML) is considered the most promising method to solve electrical contact issues impeding organic thin-film transistors (OTFTs) from commercialization. However, this method raises many concerns because MoO3 might diffuse into organic materials, which causes device instability. In this work, we observed a significant device stability degradation by damaging on/off switching performance caused by MoO3 diffusion. To prevent the MoO3 diffusion, a source-drain electrode with a multilayered interface contact (MIC) consisting of a top-down stack of metal, MoO3 IML, and organic buffer layer (OBL) is proposed. In the MIC device, the MoO3 IML serves well for its intended functions of reducing contact resistance and suppressing minority carrier injection to the OTFT channel. The inclusion of OBL to the MIC helps block MoO3 diffusion and thereby leads to better device stability and an increased on/off ratio. Through combinations with several organic compounds as a buffer layer, the MoO3 diffusion related electrical behaviors of OTFTs are systematically studied. Key parameters related to MoO3 diffusion such as the Fick coefficient and bias-stress stability such as carrier trapping time are extracted from numerical device analysis. Finally, we summarize a general rule of material selection for making robust source-drain contact.

15.
Anesth Analg ; 136(1): 34-42, 2023 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-36534715

RESUMO

BACKGROUND: Surgery is accompanied by a systemic inflammatory response that may presage delirium in susceptible individuals. Little is known about the trajectory of plasma proinflammatory cytokines and their potential associations with postoperative delirium (POD). The current study longitudinally assessed both pro and anti-inflammatory plasma cytokine response and development of POD in older surgical patients to investigate associations with individual and/or clusters of cytokines that may indicate pathogenic mechanisms. METHODS: A prospective longitudinal study sought to enroll patients >60 years old who were scheduled for major lower limb surgery under general anesthesia. Blood was obtained preoperatively and postoperatively from day 1 through postoperative day 4 for measurement of plasma interleukin-1ß (IL-1ß), IL-2, IL-4, IL-6, soluble IL-6 receptor (sIL-6R), IL-10, and tumor necrosis factor-α (TNF-α). Participants were assessed for POD twice daily for 4 days using the confusion assessment method. Trajectory of postoperative changes in plasma cytokines was determined by a group-based trajectory modeling analysis that was informed by distinct cytokines identified by time-dependent Cox regression model. RESULTS: One hundred eighty-eight patients were assessed for eligibility of whom 129 underwent major surgery and 126 had complete datasets for final analysis. POD was diagnosed in 31 of 126 patients (24.6%). Time-dependent Cox regression model identified that higher IL-6 and sIL-6R levels were associated with higher risk of developing POD. A two-cluster model (stable lower and fluctuating higher levels) was considered to be the most statistically appropriate model for IL-6 and sIL-6R trajectory. More participants with fluctuating higher IL-6 were delirious (73.3% vs 18.0%, P = .001) as were those with fluctuating higher sIL-6R (81.3% vs 16.4%, P = .001). CONCLUSIONS: As higher IL-6 and sIL-6R levels were significantly associated with higher risk of POD and the combination is required for IL-6 trans-signaling, it is possible that activation of this pathway may be associated with POD. Furthermore, it will be important to determine whether high levels of the combination of IL-6 and sIL-6R can be an early biomarker for the subsequent development of POD.


Assuntos
Citocinas , Delírio do Despertar , Humanos , Idoso , Pessoa de Meia-Idade , Interleucina-6 , Estudos Prospectivos , Estudos Longitudinais , Extremidade Inferior
16.
ACS Appl Mater Interfaces ; 15(1): 2341-2350, 2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36576799

RESUMO

The development of aqueous zinc-ion batteries (AZIBs) has been severely restricted by metallic Zn anode issues, including Zn dendrites and uncontrollable side reactions. The direct interfacial modification of a Zn anode is a facile and effective strategy that has been favored by researchers. Herein, we propose an acrylic-bonded stationary layer as an artificial solid electrolyte interface (SEI) for the Zn anode to manipulate the Zn plating/stripping. The constructed modification layer is made of zinc acrylate cross-linked to form a three-dimensional (3D) porous structure, which can induce uniform deposition of zinc ions and balance the local electric field, thus achieving homogeneous nucleation and uniform Zn deposition. More importantly, this fabricated layer is chemically bonded to the zinc surface and can tolerate prolonged zinc deposition without shedding compared with conventional physical coatings. Consequently, the Zn/Zn symmetric cells, Zn/Na0.92V2O5·nH2O (NaVO) full cells, and Zn hybrid supercapacitors using this acrylic-bonded modified anode demonstrate greatly improved cycle performance.

17.
Nanomicro Lett ; 15(1): 5, 2022 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-36472752

RESUMO

Ferroelectric polymer nanocomposites possess exceptional electric properties with respect to the two otherwise uniform phases, which is commonly attributed to the critical role of the matrix-particle interfacial region. However, the structure-property correlation of the interface remains unestablished, and thus, the design of ferroelectric polymer nanocomposite has largely relied on the trial-and-error method. Here, a strategy that combines multi-mode scanning probe microscopy-based electrical characterization and nano-infrared spectroscopy is developed to unveil the local structure-property correlation of the interface in ferroelectric polymer nanocomposites. The results show that the type of surface modifiers decorated on the nanoparticles can significantly influence the local polar-phase content and the piezoelectric effect of the polymer matrix surrounding the nanoparticles. The strongly coupled polar-phase content and piezoelectric effect measured directly in the interfacial region as well as the computed bonding energy suggest that the property enhancement originates from the formation of hydrogen bond between the surface modifiers and the ferroelectric polymer. It is also directly detected that the local domain size of the ferroelectric polymer can impact the energy level and distribution of charge traps in the interfacial region and eventually influence the local dielectric strength.

18.
Curr Res Food Sci ; 5: 2316-2328, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36467746

RESUMO

A microbial starter culture is expected to improve the quality of traditional fermented fish products. Lactobacillus plantarum was selected for grass carp fermentation due to its high proteolytic activity. To investigate its effects on muscle proteolysis of dried fermented fish, the protein profile and microbial community were analysed by using proteomics and metabolomics. The myofibrillar protein and collagen profiles showed remarkable variation after processing, changes that were related to the development of flavour and texture in fish samples. The starter culture had a marked effect on the microbial composition. Macrococcus and Staphylococcus were the dominant genera, with a relative abundance of 24.79% and 12.53%, respectively. There were significant correlations (P < 0.05) between the dominant genera and the major peptidase genes and quality-related proteins. These findings suggest that microbial activity is involved in proteolysis and affects the flavour and texture of dried fermented fish.

19.
Environ Toxicol ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36454672

RESUMO

Human discs large-associated protein 5 (DLGAP5), a microtubule-associated protein, has been reported to be upregulated in several tumors. However, the role of DLGAP5 in endometrial cancer (EC) progression and the related underlying mechanism were still unknown. A bioinformatics analysis was performed to analyze the expression and prognostic significance of DLGAP5 in EC tissues using TCGA, CPTAC, Human Protein Atlas, and GSE63678 databases, UALCAN web tool, and the Kaplan-Meier plotter. Effects of DLGAP on EC cell malignant properties were evaluated by CCK-8, flow cytometry analysis, TUNEL assay, caspase-3 activity assay, and Transwell invasion assay. The expression of DLGAP5, Wnt3, c-Myc, Ki67, and cleaved caspase-3 was detected by western blot analysis. DLGAP5 was highly expressed and correlated with poor prognosis in EC patients. DLGAP5 knockdown inhibited proliferation and invasion, triggered apoptosis, and increased caspase-3 activity in EC cells. Additionally, DLGAP5 knockdown inactivated the Wnt/ß-catenin signaling pathway in EC cells. Moreover, ß-catenin overexpression abolished the effects of DLGAP5 knockdown on the malignant phenotypes of EC cells. DLGAP5 silencing suppressed the malignant properties in EC cells by inactivating the Wnt/ß-catenin pathway.

20.
Front Psychol ; 13: 1041630, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36506983

RESUMO

This paper aims to examine the impact of CEOs' early-life disaster experiences on corporate earnings quality. We proxy the disaster experience with whether CEOs lived through the Great Chinese Famine and the famine intensity they experienced. The results indicate that CEOs' early-life famine experience is significantly positively associated with corporate earnings quality, and the famine effects are more obvious for CEOs who experienced the famine at adolescent ages. Further tests show that the famine experience effects are more pronounced in companies with high investor protection and cross-listing and with CEOs who have a relatively high level of education or background in economic management. The findings suggest CEOs would bear the imprint of an adverse early-life experience, which has risk aversion and learning effects on their decision making in corporate earnings information disclosure.

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