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1.
Aging (Albany NY) ; 14(12): 5177-5194, 2022 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-35749138

RESUMO

Long non-coding RNAs (lncRNAs) are reportedly involved in the regulation of physiological and pathophysiological processes. However, the potential role of lncRNAs in stroke remains largely undefined. Here, RNA-Seq analysis of lncRNAs found that the lncRNA PEG11as (PEG11as) levels were significantly increased in ischemic brain tissue in a transient middle cerebral artery occlusion/reperfusion (tMCAO/R) mouse model of stroke. To explore the role of PEG11as in stroke, the lentivirus containing PEG11as silencing construct(siRNA-PEG11as) was microinjected intracerebroventricularly into male or transfected to N2a cells and then exposed to tMCAO/R or oxygen-glucose deprivation/reoxygenation (OGD/R). Knockdown of PEG11as expression significantly reduced infarct volume, alleviated neuronal deficits and inhibited neuronal apoptosis in tMCAO/R mice. Mechanistically, as an endogenous microRNA-874-3p (miR-874-3p) sponge, PEG11as silencing inhibited miR-874-3p activity, resulting in downregulation of ATG16L1 expression and subsequent inhibition of neuronal apoptosis by regulating autophagy. Overall, the results of this current study indicate that PEG11as is involved in the pathophysiology of cerebral ischemia, thus providing translational evidence that PEG11as can be envisioned as a novel biomarker or/and therapeutic target for stroke.


Assuntos
Isquemia Encefálica , AVC Isquêmico , MicroRNAs , RNA Longo não Codificante , Traumatismo por Reperfusão , Acidente Vascular Cerebral , Animais , Apoptose/fisiologia , Autofagia/fisiologia , Isquemia Encefálica/genética , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Glucose/metabolismo , Infarto da Artéria Cerebral Média/genética , Infarto da Artéria Cerebral Média/metabolismo , Infarto da Artéria Cerebral Média/patologia , AVC Isquêmico/genética , AVC Isquêmico/metabolismo , AVC Isquêmico/patologia , Masculino , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Longo não Codificante/genética , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Acidente Vascular Cerebral/genética , Acidente Vascular Cerebral/metabolismo , Acidente Vascular Cerebral/patologia
2.
World J Clin Cases ; 10(11): 3490-3495, 2022 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-35611211

RESUMO

BACKGROUND: Cladosporium and Corynespora cassiicola (C. cassiicola) infections rarely occur in humans. Mutations in human caspase recruitment domain protein 9 (CARD9) are reported to be associated with fungal diseases. Pulmonary Cladosporium infection coexisting with subcutaneous C. cassiicola infection in a patient with a CARD9 mutation has not been reported in the literature. CASE SUMMARY: A 68-year-old male patient was hospitalized for hypertrophic erythema and deep ulcers on the left upper extremity. He was diagnosed with pneumonia caused by Cladosporium, as identified through bronchoalveolar lavage fluid analysis, and deep dermatophytosis caused by C. cassiicola, as identified through morphological characteristics of the wound secretion culture. He underwent antifungal therapy (voriconazole) and recovered successfully. He carried two mutations in CARD9 (chr9:139266425 and chr9:139262240) and was therefore susceptible to fungal infections. CONCLUSION: This case study is the first to report the coexistence of pulmonary Cladosporium infection and subcutaneous C. cassiicola infection in a patient with CARD9 mutation. Our findings will be helpful in enriching the phenotypic spectrum of fungal infections underlying CARD9 deficiency.

3.
J Hazard Mater ; 384: 121367, 2020 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-31629589

RESUMO

Generally, the efficiency of water purification can be greatly increased by a high-flux membrane separation technology. One major challenge in the application of this technology is to achieve high removal efficacy of target pollutants with elevated water flux. Here we report a novel self-assembled composite by depositing two-dimensional MXene nanosheets on a commercialized mixed cellulose ester filter (as designated as MCM). Morphology study reveals that MCM exhibits an ultrathin flaked structure with uniform nanochannels which is stapled on a porous support. The tailored membrane has been successfully applied in the methylene blue solution treatment and 100% ± 0.1% removal rate is achieved while the feed concentration of dye solution is up to 90 mg·L-1. Concurrently, stable and comparatively elevated water flux was achieved, i.e., 28.94 ± 0.74 L·m-2·h-1, which is 1.88-fold of that of the commercialized UTC60 membrane. Further investigations on the separation mechanism are performed to get more insights into separation performance exhibited by MCM. It is found that the size-selective sieving, electrostatic repulsion of MXene and the high porosity of substrate play the synergistic effect on the fast and efficient dye removal behavior. Taken together, the composite membrane fabricated in present work provides an alternatively high-efficiency approach for dye treatment, and unflagging efforts will be further invested on the development and large-scale application of MXene-based membrane.

4.
Appl Bionics Biomech ; 2020: 8850036, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33425006

RESUMO

This study proposes a modified quasisteady aerodynamic model for the sub-100-milligram insect-inspired flapping-wing robot presented by the authors in a previous paper. The model, which is based on blade-element theory, considers the aerodynamic mechanisms of circulation, dissipation, and added-mass, as well as the inertial effect. The aerodynamic force and moment acting on the wing are calculated based on the two-degree-of-freedom (2-DOF) wing kinematics of flapping and rotating. In order to validate the model, we used a binocular high-speed photography system and a customized lift measurement system to perform simultaneous measurements of the wing kinematics and the lift of the robot under different input voltages. The results of these measurements were all in close agreement with the estimates generated by the proposed model. In addition, based on the model, this study analyzes the 2-DOF flapping-wing dynamics of the robot and provides an estimate of the passive rotation-the main factor in generating lift-from the measured flapping kinematics. The analysis also reveals that the calculated rotating kinematics of the wing under different input voltages accord well with the measured rotating kinematics. We expect that the model presented here will be useful in developing a control strategy for our sub-100 mg insect-inspired flapping-wing robot.

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