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1.
Zhonghua Xue Ye Xue Za Zhi ; 42(10): 800-806, 2021 Oct 14.
Artigo em Chinês | MEDLINE | ID: mdl-34788918

RESUMO

Objective: Factors influencing the prognosis of hemophagocytic lymphohistiocytosis (HLH) in adults were analyzed based on multicentric data. Methods: Clinical data of 124 adult patients with HLH diagnosed in eight medical centers in the Huaihai Lymphoma Working Group from March 2014 to July 2020 were collected. The optimal truncation value of continuous variables was obtained based on the Maxstat algorithm, X-Tile software, and restricted cubic spline. Cox proportional risk regression model was used to construct the adult HLH risk prediction model, and the visualization of the model was realized through the histogram. The bootstrap resampling method was used to verify the model, C-index and calibration curve was used to verify the histogram, and the prediction accuracy was checked. Kaplan-Meier analysis was used to calculate the survival rate and draw the survival curve. Furthermore, the differences between groups were tested by log-rank. Results: The median age of the 124 patients was 55 (18-84) years, including 61 (49.19%) males. The most common etiology was infection. Serum ferritin increased in 110 cases (88.71%) , hepatosplenomegaly in 57 cases (45.97%) . Of the 124 patients, 77 (62.10%) died, and the median survival time of the patients was 7.07 months. Univariate results showed that the prognosis of adult HLH was influenced by sex, age, fibrinogen, serum creatinine, alanine aminotransferase, and albumin (P<0.05) . The results of multivariate analysis showed that gender, platelet, albumin, alanine aminotransferase, and treatment regimens were independent influencing factors for prognosis. Based on the above five risk factors, the prediction model of the histogram was established, and the C-index of the model was 0.739. Finally, the calibration chart showed good consistency between the observed and predicted values of HLH. Conclusion: The prognosis of the adult hemophagocytic syndrome is influenced by many factors. Gender, platelet, albumin, alanine aminotransferase, and treatment regimens are independent risk factors. Therefore, the established histogram provides a visual tool for clinicians to evaluate the prognosis of adult HLH.


Assuntos
Linfo-Histiocitose Hemofagocítica , Linfoma , Adulto , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos
2.
Opt Express ; 29(19): 30223-30236, 2021 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-34614749

RESUMO

High-quality ultrashort electron beams have diverse applications in a variety of areas, such as 4D electron diffraction and microscopy, relativistic electron mirrors and ultrashort radiation sources. Direct laser acceleration (DLA) mechanism can produce electron beams with a large amount of charge (several to hundreds of nC), but the generated electron beams usually have large divergence and wide energy spread. Here, we propose a novel DLA scheme to generate high-quality ultrashort electron beams by irradiating a radially polarized laser pulse on a nanofiber. Since electrons are continuously squeezed transversely by the inward radial electric field force, the divergence angle gradually decreases as electrons transport stably with the laser pulse. The well-collimated electron bunches are effectively accelerated by the circularly-symmetric longitudinal electric field and the relative energy spread also gradually decreases. It is demonstrated by three-dimensional (3D) simulations that collimated monoenergetic electron bunches with 0.75° center divergence angle and 14% energy spread can be generated. An analytical model of electron acceleration is presented which interprets well by the 3D simulation results.

3.
Int J Radiat Oncol Biol Phys ; 111(3S): e515, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701637

RESUMO

PURPOSE/OBJECTIVE(S): Heart failure remains a major source of morbidity and mortality worldwide. Left ventricular assist devices (LVADs) provide mechanical circulatory support and offer superior outcomes to medical therapy in patients with end-stage heart failure. LVADs can be used as a bridge to cardiac transplantation but are now more commonly used as destination therapy. While the safety of photon beam and older proton beam therapy (PBT) techniques have been established in patients with LVADs, data evaluating the safety of pencil beam scanning (PBS) PBT techniques are lacking. In this study, we evaluated the dose tolerance of an LVAD to PBS proton irradiation. We hypothesized that proton irradiation would affect the function of the LVAD. MATERIALS/METHODS: We conducted three experiments in which we exposed elements of separate LVADs to a proton beam whose Spread Out Bragg Peak (SOBP) had a range of 100 mm and a width of 60 mm. The LVAD was placed in a water-based phantom with a wall of water-equivalent thickness of 11.59 mm at a depth of 5 cm. In the first experiment, we delivered escalating doses of proton beam radiation to the device pump, starting at 5 GyE and increasing in increments of 5 GyE up to 70 GyE. In replicative second and third experiments, we delivered proton beam radiation continuously to a dose of 70 GyE (prescribed to the middle of the SOBP) to the pump and driveline, respectively. Optical stimulated luminescent dosimeters (OSLDs) were utilized to monitor dose to the battery and controller, which were located outside of the field of radiation. Our institutional review board deemed this study exempt. RESULTS: The pump showed no significant operational changes after irradiation in the first and second experiments. However, during continuous irradiation of the driveline in the third experiment, communication between the pump and monitor was lost at 30 GyE (confirmed on the device's log files). While the pump continued to run during this time, the monitor displayed a low flow alarm. OSLD measurements were 0.02 cGy for the monitor and 0.069 cGy for the battery after the completion of the third experiment. No mechanical damage was noted to the components of the LVAD after any of the three experiments. CONCLUSION: The pump and driveline of the LVAD exhibited no structural damage after direct irradiation by a proton beam. The pump tolerated escalating doses of up to 70 GyE, which is consistent with prior published data. However, when the driveline was irradiated continuously, the controller and pump lost connection at 30 GyE, and a low flow alarm was generated. These results suggest that while the LVAD pump can tolerate clinically significant doses of PBS PBT, further study is necessary to assess the resilience of the driveline to fractionated PBT.

4.
Int J Radiat Oncol Biol Phys ; 111(3S): e616-e617, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701885

RESUMO

PURPOSE/OBJECTIVE(S): Cervical cancer is mainly treated by surgery, radiotherapy and chemotherapy. However, there are also some patients with treatment failure, such as residual tumors, recurrence or metastasis within a short period of time after the initial treatment. Apatinib is a targeted drug for a small molecule vascular endothelial growth factor receptor-2 (VEGFR-2) tyrosine kinase inhibitor. We aimed to assess the efficacy and safety of apatinib combined with capecitabine in patients with recurrent/metastatic and persistent cervical cancer after radiochemotherapy. MATERIALS/METHODS: In this phase Ⅱ, single-arm, prospective study done in Guizhou province in China, eligible patients were aged 18-70 years, had an ECOG performance status of 0 or 1, progressed after at least one line of radiochemotherapy for metastatic, recurrent or persistent cervical cancer, and had measurable lesions. Patients received capecitabine 2000mg/m2 divided into morning and evening every day and apatinib 250mg once daily. Treatment continued until disease progression, unacceptable toxicity, and withdrawal of consent. The primary endpoint was the objective response rate (ORR) assessed by RECIST version 1.1 and Progression-free survival (PFS). Disease control rate (DCR), overall survival (OS),1-year survival rate and safety were the second endpoints. RESULTS: Between August 2018 and May 2020, 28 patients were enrolled and received study treatment. The median age was 52(range, 34-67) years. The median previous treatment lines were 2 (range, 2-5). As of Jan 1, 2021, median follow-up was 10.18 months (range, 3.4-29). 12 (42.9%; 95%: CI 23.5-62.3) of 28 patients who had at least one post-baseline tumor assessment (efficacy evaluation population) achieved an objective response, including 3 (10.7%) complete response (CR), and 9 (32.1%) partial response (PR). The median duration of response was 1.57months(range,0.9-2.17). The DCR was 82.1% (95% CI: 67.3% -97.2%). Median progression-free survival (PFS) was 4.7 months (95% CI: 1.2-18.6). The one-year survival rate was 54.8%, and the longest administration time was 18.2 months.10 (35.7%) patients had grade ≥ 3 treatment-related adverse events (TRAEs). Grade ≥ 3 TRAEs occurring in ≥ 5% of patients were hypertension (7.1%), emesis (7.1%), myelosuppression (7.1%). Notably, one patient discontinued maintenance therapy after 13 months because of coronary artery stenosis which is a rare toxic side effect of apatinib. CONCLUSION: Apatinib combined with capecitabine showed promising antitumor activity and tolerable toxicities in patients with recurrent/metastatic and persistent cervical cancer after chemotherapy.

5.
Int J Radiat Oncol Biol Phys ; 111(3S): e616, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34701886

RESUMO

PURPOSE/OBJECTIVE(S): The standard treatment for locally advanced cervical cancer was concurrent chemoradiotherapy. The effect of concurrent chemoradiotherapy was poor in locally advanced cervical cancer (lump ≥4 cm) (LACC) (lump ≥4 cm). We conduct this research in order to explore the role of neoadjuvant chemotherapy combined with concurrent radiotherapy in LACC (lump ≥4 cm). MATERIALS/METHODS: A phase III, prospective, randomized controlled clinical trial was conducted at Guizhou Cancer Hospital. LACC (lump ≥4 cm) (FIGO 2018) were randomly divided into two groups, neoadjuvant chemotherapy combined with concurrent chemoradiotherapy (NACT+CCRT) group and concurrent chemoradiotherapy (CCRT) group. In NACT+CCRT group, patients were treated with paclitaxel cisplatin (TP) for 2 cycles, then they received concurrent chemoradiotherapy with TP for 2 cycles. In CCRT group, the patients were treated concurrent chemoradiotherapy with TP for 2 cycles. Tumor volume shrinkage, response rate and toxicity were the primary endpoints. Progression-free survival (PFS) and overall survival (OS) were the second endpoints. RESULTS: Between March 2019 and December 2020, 97 patients randomly assigned (52 patients in the NACT+CCRT group and 45 patients in the CCRT group). In the NACT+CCRT group, after NACT, of evaluable 52, 52 responded: complete response (CR) 1(1.92%) and partial response (PR) 24(46.15%), stable disease (SD) 27(51.92%) and progressive disease (PD) 0(0%). 52 patients have completed CCRT, after NACT+CCRT, of evaluable 52, 52 responded: CR17(32.69%) and PR 35(67.31%), SD 0 and PD 0. In the CCRT group, 5 patients have not completed CCRT (because of the large tumor and concurrent radiotherapy not be tolerated), of evaluable 40, 40 responded: CR 8(20.00%) and PR 32(80.00%), SD 0 and PD 0. The immediate CR rate was better in NACT+CCRT than in the CCRT (P = 0.001). Treatment completion status: in the NACT+CRT group, all the patients (100%) have completed the NACT+CCRT, there were 40 (88.89%) patients have completed treatment (P = 0.013). In the tumor shrinkage, the median tumor volume after NACT was 33.91cm3 and 79.74cm3 in the CCRT group. There was significant difference between two groups (P = 0.000). Adverse event Hematological and gastrointestinal toxicity were most common. The Acute toxicities were hematological toxicity, gastrointestinal toxicity, there was no significant difference between two groups in the NACT+CCRT group versus the CCRT group (P>0.05). CONCLUSION: NACT can minify the tumor volume of LACC (lump≥4cm) before CCRT and reduce the radiation dose of normal organs around the target area, thereby improved the completion rate of concurrent radiotherapy treatment. After NACT+CRT, the immediate response rate was improved significantly, especially the CR. However, there were more significant adverse events during NACT+CRT but it was well tolerated by patients. The high efficiency can be transformed into the advantage of prolonging survival or not, longer follow up and further research is needed. AUTHOR DISCLOSURE: F. Li: None. J. Li: None. S. Yin: None. F. Mei: None. Y. Du: None. L. Hu: None. X. Tian: None. W. Hong: None. L. Shan: None. M. Liu: None. Y. Chen: None. W. Mao: None. J. Mu: None. B. Lu: None.

6.
Phys Rev Lett ; 127(9): 090504, 2021 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-34506165

RESUMO

The ability to manipulate quantum systems lies at the heart of the development of quantum technology. The ultimate goal of quantum control is to realize arbitrary quantum operations (AQUOs) for all possible open quantum system dynamics. However, the demanding extra physical resources impose great obstacles. Here, we experimentally demonstrate a universal approach of AQUO on a photonic qudit with the minimum physical resource of a two-level ancilla and a log_{2}d-scale circuit depth for a d-dimensional system. The AQUO is then applied in a quantum trajectory simulation for quantum subspace stabilization and quantum Zeno dynamics, as well as incoherent manipulation and generalized measurements of the qudit. Therefore, the demonstrated AQUO for complete quantum control would play an indispensable role in quantum information science.

7.
Zhonghua Er Ke Za Zhi ; 59(7): 570-575, 2021 Jul 02.
Artigo em Chinês | MEDLINE | ID: mdl-34405639

RESUMO

Objective: To explore the genetic etiologies of newborn deaths. Methods: A total of 98 newborns who were recruited to the Neonatal Genome Project of the Children's Hospital of Fudan University and died in the hospital from January 2018 to August 2020 were enrolled in this study. The genetic information and the interventions based on the genetic findings were retrospectively analyzed. T-test, Mann-Whitney U test, Chi square test and Fisher's exact probability test were used to compare the demographic features and clinical characteristics between the patients with or without a genetic finding. Results: Among 98 newborns (55 males and 43 females), there were 63 preterm and 35 term infants, with a gestational age of (33±5) weeks, a birth weight of (2 107±975) g and the age at death of 12 (2,34) days. Sixteen (16%)patients were identified with genetic variants, including 11 with single nucleotide variants, 4 with copy number variants and 1 with both single nucleotide variant and copy number variant. The detected single nucleotide variants were spanning 12 genes, among which 3 were multiple disorders-related, 2 metabolic disorder-related, 2 hematological disorder-related, 2 respiratory disorder-related, 2 cardiovascular disorder-related and 1 skeletal disorder-related. The patients with a positive genetic finding had significant differences in the birth weight ((2 605±940) vs. (2 009±957) g, t=2.283, P=0.025), the gestational age ((36±5) vs. (33±5) weeks, t=2.131, P=0.036), the age at death ((37 (5, 69) vs. 11 (2, 29) days, Ζ=-2.245, P=0.025) and the history of asphyxia at birth (1/16 vs. 46% (38/82), P=0.002)when compared to those without a genetic finding. In addition, the genetic etiology rates of patients who were born term or with a birth weight ≥ 2 000 g were significantly higher than those who were born preterm (29% (10/35) vs. 10% (6/63), P=0.022) or with a birth weight<2 000 g (25% (13/51) vs. 7% (3/46), χ2=5.016,P=0.025), respectively. Six cases were medically actionable based on the genetic findings and the treatments included special diet, applying specific medicine, hematopoietic stem cell transplantation and lung transplantation. Conclusions: Genetic etiologies are not rare in newborn deaths and mainly associated with metabolic disorder, multi-system disorders, hematological disorder, respiratory disorder, cardiovascular disorder and skeletal disorder. Some findings are medically actionable, based on which the specific treatments could be scheduled timely. A genetic etiology should be investigated in newborn deaths especially in those who are term birth or with a birth weight ≥2 000 g or without a history of asphyxia at birth.


Assuntos
Estudos Retrospectivos , Peso ao Nascer , Feminino , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Masculino , Fatores de Risco
9.
Phys Rev Lett ; 127(2): 020504, 2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34296922

RESUMO

Digital quantum simulators provide a diversified tool for solving the evolution of quantum systems with complicated Hamiltonians and hold great potential for a wide range of applications. Although much attention is paid to the unitary evolution of closed quantum systems, dissipation and noise are vital in understanding the dynamics of practical quantum systems. In this work, we experimentally demonstrate a digital simulation of an open quantum system in a controllable Markovian environment with the assistance of a single ancillary qubit. By Trotterizing the quantum Liouvillians, the continuous evolution of an open quantum system is effectively realized, and its application in error mitigation is demonstrated by adjusting the simulated noise intensities. High-order Trotter for open quantum dynamics is also experimentally investigated and shows higher accuracy. Our results represent a significant step toward hardware-efficient simulation of open quantum systems and error mitigation in quantum algorithms in noisy intermediate-scale quantum systems.

10.
Rev Sci Instrum ; 92(4): 043506, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243369

RESUMO

Stilbene crystal detectors are widely used as fast neutron measurement tools based on recoil proton detection, such as liquid scintillators. A compact stilbene crystal neutron spectrometer (CSCNS) has been installed at the Experimental Advanced Superconducting Tokamak (EAST) to obtain information on fuel ions produced in the plasma core because of its merits of good n/γ discrimination capability, high detection efficiency, and fast response. For the first time, CSCNS has been used for neutron emission spectroscopy measurements in EAST plasmas with neutral beam injection (NBI) heating. The CSCNS has the same horizontal line of sight as the time-of-flight enhanced diagnostics neutron spectrometer. Under NBI heating scenarios, the time trace of the neutron yield monitored by the CSCNS is similar to the one monitored by a standard 235U fission chamber. The experimental pulse height spectra are also similar to the simulated ones generated by folding the simulated neutron energy spectrum with the detector response functions. These results demonstrate the capability of the CSCNS for neutron diagnostics and the study of fast-ion physics in EAST.

11.
Rev Sci Instrum ; 92(4): 043511, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243396

RESUMO

The cross-polarization scattering (CPS) system for magnetic fluctuation measurements in the Experimental Advanced Superconducting Tokamak (EAST) has been designed and installed. Different from the Doppler reflectometer (DR) system, the CPS system detects the perpendicular polarization of the electromagnetic wave induced by magnetic fluctuations B̃. The CPS system in the EAST has been developed from the existing Doppler reflectometer system, and they are integrated together for simultaneous measurement of magnetic and density fluctuations. Ray-tracing simulations are used to calculate the scattering locations and the wavenumber coverage of the magnetic fluctuation for CPS. In the experiments, the CPS and DR system data were different in Doppler shift, amplitude, and spectrum broadening. In this article, the hardware design, the ray tracing, and the preliminary results of the system in the EAST are presented.

12.
Rev Sci Instrum ; 92(4): 043552, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243473

RESUMO

Neutron emission spectroscopy and neutron yield measurements are important for high neutral beam injection (NBI) power heating at the Experimental Advanced Superconducting Tokamak (EAST). The neutron yields mainly depend on the deposition from NBI to the deuterium plasmas in the EAST. We have recently used TRANSP with time dependent diagnostic results to simulate the transport process of 30 s long pulse deuterium plasma discharges in the EAST, obtaining the time dependent fast ion distribution, neutron emission spectrum, and total neutron emission rate. Combined with the time trace of the result measured by a standard 235U fission chamber, the effects of different configurations of NBI heating in EAST fusion plasmas have been evaluated.

13.
Zhonghua Fu Chan Ke Za Zhi ; 56(7): 482-488, 2021 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-34304440

RESUMO

Objective: To investigate the influence of age on the fresh cycle live birth rate in patients with poor ovarian response in different controlled ovarian hyperstimulation groups. Methods: The clinical data of 3 342 patients in The First Affiliated Hospital of Zhengzhou University from February 2014 to November 2018 were retrospectively collected, including early-follicular phase long-acting gonadotropin-releasing hormone (GnRH) agonist long protocol group (1 375 cases), mid-luteal phase short-acting GnRH agonist long protocol group (1 161 cases) and GnRH antagonist protocol group (806 cases); each group was divided into 4 subgroups according to age: ≤30 years, 31-35 years, 36-40 years and >40 years, the pregnancy outcomes in each age subgroup were analyzed under different controlled ovarian hyperstimulation protocols. Results: In early-follicular phase long-acting GnRH agonist long protocol group, the final live birth rates of each age subgroup were 39.4% (228/579), 36.1% (135/374), 16.6% (48/290) and 3.0% (4/132); in mid-luteal phase short-acting GnRH agonist long protocol group, live birth rates of each age subgroup were 32.1% (99/308), 20.8% (55/264), 13.0% (45/346) and 7.0% (17/243); in GnRH antagonist protocol group, live birth rates of each age subgroup were 22.8% (26/114), 16.3% (25/153), 11.2% (31/278), and 3.8% (10/261); the live birth rate of each group decreased significantly with the increase of age (all P<0.01). When the age≤35 years old, the fresh cycle live birth rate of the early-follicular phase long-acting GnRH agonist long protocol group was significantly better than those of the other two groups (all P<0.01). The multivariate logistic regression analysis of age and live birth rate of the three controlled ovarian hyperstimulation groups showed age was the independent influence factor (OR=0.898, 95%CI: 0.873-0.916, P<0.01; OR=0.926, 95%CI: 0.890-0.996, P<0.01; OR=0.901, 95%CI: 0.863-0.960, P<0.01). Conclusions: Age is an independent influencing factor for the prediction of fresh cycle live birth rate in low ovarian response patients. No matter which controlled ovarian hyperstimulation protocol is adopted, the final live birth rate decreases significantly with the increase of women's age. In addition, the early-follicular phase long-acting GnRH agonist long protocol has the highest fresh cycle live birth rate among all controlled ovarian hyperstimulation groups.


Assuntos
Coeficiente de Natalidade , Indução da Ovulação , Adulto , Feminino , Fertilização In Vitro , Hormônio Liberador de Gonadotropina , Humanos , Nascido Vivo/epidemiologia , Gravidez , Taxa de Gravidez , Estudos Retrospectivos
14.
Eur Rev Med Pharmacol Sci ; 25(10): 3700-3708, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-34109579

RESUMO

OBJECTIVE: This meta-analysis aimed to assess the association of MUC-2 expression with clinicopathological parameters in gastric carcinoma (GC) patients. MATERIALS AND METHODS: Clinical databases based on the study aim were searched in detail. The relative risk ratios (RRs) and associated 95% confidence intervals (95% CIs) were computed after eligible trials were included in the study. RESULTS: Nineteen trials involving 2,363 GC patients were included in this meta-analysis. The expression of MUC-2 showed correlation with clinical stage (I/II vs. III/IV) (RR = 1.09, 95% CI: 1.00-1.18, I2 = 24%, p = 0.194), and lymphatic invasion (present vs. absent) (RR = 0.83, 95% CI: 0.72-0.95, I2 = 22.3%, p = 0.252). However, no significant association was identified between the MUC-2 expression and other clinicopathological parameters, including gender (male vs. female), tumor size (>5 vs. ≤5 cm), Lauren's classification (intestinal vs. diffuse), tumor differentiation (poorly vs. well and moderately), lymph node metastasis (present vs. absent), vascular invasion (present vs. absent), and 5-year survival (yes vs. no) of GC patients. CONCLUSIONS: Our meta-analysis findings suggested that MUC-2 positive cases were correlated with lower tumor stage and lower rate of lymphatic invasion. Further clinical studies are warranted to confirm the role of MUC-2 in clinical practice.

15.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 56(6): 549-556, 2021 Jun 09.
Artigo em Chinês | MEDLINE | ID: mdl-34098670

RESUMO

Objective: To investigate the effects of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) of type Ⅰand Ⅳ fimA on the proliferation and migration of human umbilical artery smooth muscle cells (HUASMC) under co-culture conditions and to explore the biological basis and possible mechanisms of the relationship between periodontitis and atherosclerosis (As). Methods: Type Ⅰ and Ⅳ fimA Pg were anaerobically cultured and the Pg-LPS was extracted, purified and identified. Human umbilical vein endothelial cells (HUVEC) and HUASMC were cultured in vitro and the HUVEC-HUASMC co-cultured cell model was established using rat tail type Ⅰ collagen. The experiment was divided into three groups: group T1 (co-cultured cells were stimulated with type Ⅰ fimA Pg-LPS at the mass concentrations of 0.5, 1.0, 2.0, 5.0, 10.0 mg/L), group T2 (co-cultured cells were stimulated with type Ⅳ fimA Pg-LPS at the mass concentrations of 0.5, 1.0, 2.0, 5.0, 10.0 mg/L), and negative control group (without LPS). Cell counting kit-8 (CCK-8) was used to detect the proliferation ability of HUASMC and the migration ability of HUASMC was observed by using the Transwell migration chamber. Comparasons of the changes in the proliferation and migration ability of HUASMC after 2, 8, 24 and 48 h of Pg-LPS stimulated co-cultured cells with various mass concentrations of Pg-LPS were conducted. Results: For the co-cultured cells under the action of type Ⅰ and Ⅳ fimA Pg-LPS, at 24 and 48 h, in each mass concentration group of the two types of Pg-LPS (0.5, 1.0, 2.0, 5.0, 10.0 mg/L), HUASMC's A values were significantly up-regulated compared to the negative control group (P<0.05) and for the co-cultured cells after the stimulation of type Ⅳ fimA Pg-LPS at concentrations of 5 and 10 mg/L at 48 h, the A values (1.386±0.044, 1.455±0.058) of HUASMC were significantly higher than that of HUASMC (1.168±0.064, 1.204±0.088) in the same concentrations of type Ⅰ fimA Pg-LPS (P<0.05). In addition, the A values of HUASMC in stimulated co-cultured cells under concentrations of 5.0 and 10.0 mg/L of type Ⅳ fimA Pg-LPS at 48 h were significantly higher than that in stimulated co-culture cells under the concentrations of 0.5 and 1.0 mg/L of type Ⅳ fimA Pg-LPS (1.170±0.082, 1.239±0.089) (P<0.05). The migration results of HUASMC showed that at 8, 24, and 48 h, the numbers of migration of HUASMC in each mass concentration group of type Ⅰ and Ⅳ fimA Pgas-LPS were significantly higher than that of HUASMC under the same Pg-LPS mass concentration at 2 h in the same group (P<0.05). The migration quantities of HUASMC in the other Pg-LPS mass concentration groups at 48 h were significantly higher than that of HUASMC under the same Pg-LPS mass concentration at 24 h in the same group (P<0.05), except for 10.0 mg/L type Ⅳ fimA Pg-LPS. The numbers of HUASMC migration in 2.0 mg/L type Ⅳ fimA Pg-LPS stimulated co-cultured cells at 48 h (204.00±20.98) were significantly higher than that in type Ⅰ fimA Pg-LPS stimulated co-culture cells at the same concentration (141.89±18.28) (P<0.05). Further more, at the same observation time point, the higher the Pg-LPS concentration, the greater the number of HUASMC migration (P<0.05). Conclusions: Both Ⅰ and Ⅳ fimA Pg-LPS could enhance the proliferation and migration ability of HUASMC in the co-culture system, and the virulence of Pg-LPS might be related to the fimA genotype. Ⅳ fimA Pg-LPS showed a more significant stimulating effect and more likely to cause HUASMC dysfunction than Ⅰ fimA Pg-LPS, which provided part of the basis for the progression of As in severe periodontitis.


Assuntos
Lipopolissacarídeos , Porphyromonas gingivalis , Animais , Proliferação de Células , Técnicas de Cocultura , Humanos , Miócitos de Músculo Liso , Ratos , Artérias Umbilicais
16.
Eur Rev Med Pharmacol Sci ; 25(6): 2457, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33829426

RESUMO

This paper presents several inaccuracies and mistakes. Therefore, the article "MicroRNA-124 inhibits proliferation and metastasis of esophageal cancer via negatively regulating NRP1, by R.-K. Zang, J.-B. Ma, Y.-C. Liang, Y. Wang, S.-L. Hu, Y. Zhang, W. Dong, W. Zhang, L.-K. Hu, published in Eur Rev Med Pharmacol Sci 2018; 22 (14): 4532-4541-DOI: 10.26355/eurrev_201807_15508-PMID: 30058693" has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/15508.

17.
Zhonghua Shao Shang Za Zhi ; 37(4): 340-349, 2021 Apr 20.
Artigo em Chinês | MEDLINE | ID: mdl-33887882

RESUMO

Objective: To explore the epidemiological characteristics and treatment outcomes of patients with inhalation injuries combined with total burn area less than 30% total body surface area (TBSA). Methods: A retrospective observational study was performed on medical records of 266 patients with inhalation injuries combined with total burn area less than 30%TBSA who were admitted to the First Affiliated Hospital of Naval Medical University from January 2008 to December 2016 and met the inclusion criteria. The following statistical data of the patients were collected, including gender, age, injury site, injurious factors of inhalation injury, degree of inhalation injury, combined total burn area, tracheotomy, time of tracheotomy, mechanical ventilation, whether stayed in intensive care unit (ICU) or not, microbial culture results of bronchoalveolar lavage fluid, length of hospital stay, length of ICU stay, mechanical ventilation days, and respiratory tract infections. Single factor and multivariate linear regression analysis were used to screen out the risk factors impacting the length of hospital stay, length of ICU stay, and mechanical ventilation days of patients. Single factor and multivariate logistic regression analysis were used to screen out the risk factors impacting respiratory tract infections of patients. Results: The 266 patients included 190 males and 76 females, with the majority age of above or equal to 21 years and below 65 years (217 patients). The major injury site was confined space. The major factor causing inhalation injury was hot air. Mild and moderate inhalation injuries were more common in patients. The combined total burn area was 9.00% (3.25%, 18.00%) TBSA. In 111 patients who had tracheotomy, most of them received the procedures before being admitted to the First Affiliated Hospital of Naval Medical University. The length of hospital stay of patients was 27 (10, 55) days. The length of ICU stay of 160 patients who were hospitalized in ICU was 15.5 (6.0, 40.0) days. The mechanical ventilation days of 109 patients who were conducted with mechanical ventilation were 6.0 (1.3, 11.5) days. A total of 119 patients were diagnosed with respiratory tract infections, with 548 strains including 35 types of pathogens isolated, mainly Gram-negative bacteria. Single factor linear regression analysis showed that age, injurious factors of inhalation injury, combined total burn area, degree of inhalation injury (moderate and severe), tracheotomy, mechanical ventilation, and respiratory tract infections were the factors impacting the length of hospital stay of patients (ß=-0.198, -0.224, 0.021, 0.127, 0.164, -0.298, 0.357, 0.447, 95% confidence interval (CI)=-0.397--0.001, -0.395--0.053, 0.015-0.028, 0.009-0.263, 0.008-0.319, -0.419--0.176, 0.242-0.471, 0.340-0.555, P<0.1). Multivariate linear regression analysis showed that with mechanical ventilation and respiratory tract infections were the independent risk factors impacting the length of hospital stay of patients (ß=0.146, 0.383, 95% CI=0.022-0.271, 0.261-0.506, P<0.05 or P<0.01). Single factor linear regression analysis showed that injurious factors of inhalation injury, combined total burn area, degree of inhalation injury (moderate and severe), tracheotomy (no tracheotomy and prophylactic tracheotomy), mechanical ventilation, and respiratory tract infections were the factors impacting the length of ICU stay of patients (ß=0.225, 0.008, 0.237, 0.203, -0.408, -0.334, 0.309, 0.523, 95% CI=0.053-0.502, 0.006-0.010, -0.018-0.457, -0.022-0.428, -0.575--0.241, -0.687--0.018, 0.132-0.486, 0.369-0.678, P<0.1). Multivariate linear regression analysis showed that with respiratory tract infections was the independent risk factor impacting the length of ICU stay of patients (ß=0.440, 95% CI=0.278-0.601, P<0.01). Single factor linear regression analysis showed that injury site, injurious factors of inhalation injury (smoke and chemical gas), combined total burn area, degree of inhalation injury (moderate and severe), tracheotomy (no tracheotomy and prophylactic tracheotomy), and respiratory tract infections were the factors impacting mechanical ventilation days of patients (ß=-0.300, 0.545, 0.163, 0.005, 0.487, 0.799, -0.791, -0.736, 0.300, 95% CI=-0.565--0.034, 0.145-0.946, 0.051-1.188, 0.001-0.009, 0.127-0.847, 0.436-1.162, -1.075--0.508, -1.243--0.229, 0.005-0.605, P<0.1). Multivariate linear regression analysis showed that smoke inhalation, severe inhalation injury, and respiratory tract infections were the independent risk factors impacting mechanical ventilation days of patients (ß=0.210, 0.495, 0.263, 95% CI=0.138-0.560, 0.143-0.848, 0.007-0.519, P<0.05 or P<0.01). Single factor logistic regression analysis showed that age, injury site, combined total burn area (10%-19%TBSA and 20%-29%TBSA), degree of inhalation injury (moderate and severe), tracheotomy (prophylactic tracheotomy and no tracheotomy), and mechanical ventilation were the factors impacting respiratory tract infections of patients (odds ratio=1.079, 0.815, 1.400, 1.331, 1.803, 1.958, 0.990, 0.320, 3.094, 95% CI=0.840-1.362, 0.641-1.044, 1.122-1.526, 1.028-1.661, 1.344-2.405, 1.460-2.612, 0.744-1.320, 0.241-0.424, 2.331-4.090, P<0.1). Multivariate logistic regression analysis showed that with mechanical ventilation was the independent risk factor impacting respiratory tract infections of patients (odds ratio=4.300, 95% CI=2.152-8.624, P<0.01). Conclusions: The patients with inhalation injuries combined with total burn area less than 30%TBSA are mainly young and middle-aged males. Smoke inhalation, degree of inhalation injury, with mechanical ventilation and respiratory tract infections are the factors that affect the outcomes of patients with inhalation injuries combined with total burn area less than 30%TBSA. Additionally, prophylactic tracheotomy shows its potential value in reducing respiratory tract infections in patients with moderate or severe inhalation injuries.


Assuntos
Queimaduras , Lesão por Inalação de Fumaça , Superfície Corporal , Queimaduras/epidemiologia , Feminino , Hospitalização , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Lesão por Inalação de Fumaça/epidemiologia
18.
Zhonghua Jie He He Hu Xi Za Zhi ; 44(3): 213-217, 2021 Mar 12.
Artigo em Chinês | MEDLINE | ID: mdl-33721934

RESUMO

Objective: To study the correlation between systemic inflammation level and emphysema degree and bone mineral density in chronic obstructive pulmonary disease (COPD) patients and its possible mechanism. Methods: 90 patients with stable COPD who met the inclusion criteria and 50 controls in the physical examination center during the same period were recruited. All the enrolled objects have collected general clinical data, analyzed peripheral blood samples, measuring the Low-attenuation area of lung and CT value of lumbar 1 vertebra (L1-CT) by chest spiral CT. According to LAA%, COPD patients were divided into 36 cases of the non-emphysema group, 32cases of mild to moderate emphysema group, and 22 cases of severe emphysema group. The correlation between L1-CT value, LAA%, peripheral blood inflammatory factors, and pulmonary function indices in each group was analyzed and compared. Results: The HU value of L1-CT (107±32) in the COPD group was significantly lower than that in the control group (153±30), and the difference was statistically significant (P<0.05). The higher the LAA% in COPD patients was, the lower the value of L1-CT was, and the difference between groups was statistically significant. Compared with COPD patients in the non-emphysema group, peripheral blood neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), and C-reactive protein (CRP) were all increased in the emphysema group, and the differences were statistically significant (P<0.05). L1-CT was negatively correlated with LAA, PLR, NLR, and CRP while uncorrelated with serum concentration of calcium and phosphorus. Conclusion: The decrease in bone density in COPD patients is closely related to the degree of emphysema. It is associated with increased levels of systemic inflammation caused by COPD itself. Early and timely broad-spectrum anti-inflammatory treatment may have certain clinical significance for the prevention and treatment of comorbidity with osteoporosis.


Assuntos
Enfisema , Doença Pulmonar Obstrutiva Crônica , Enfisema Pulmonar , Densidade Óssea , Humanos , Inflamação/diagnóstico por imagem , Doença Pulmonar Obstrutiva Crônica/complicações , Doença Pulmonar Obstrutiva Crônica/diagnóstico por imagem , Enfisema Pulmonar/diagnóstico por imagem
19.
An Acad Bras Cienc ; 93(1): e20181159, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33681949

RESUMO

The analysis of landscape pattern change is an effective way to understand urbanization. Research area is the core area of urbanization, which is between Zhengzhou city and Kaifeng city in China. Geographic information system (GIS) and landscape metrics method are used to quantify the gradient landscape pattern changes in the study area. The results show an obvious gradient change of landscape pattern from 2005 to 2015. The landscape pattern in the eastern and western of the study area experienced larger changes than the middle area. The western end mainly changed in terms of the patch number, patch shape and anthropogenic influence, but the patch types in the east changed more rapidly than those in the west. Landscape pattern change is influenced by policy and location, and human influence is the main driving force. Unlike previous study areas, in which urbanization expanded from the center of a city to its surroundings, our study area spread from two ends (Zhengzhou and Kaifeng cities) to the center of the study area. This study could be used as a typical case for the gradient analysis of landscape pattern changes during urbanization.


Assuntos
Ecossistema , Urbanização , China , Cidades , Conservação dos Recursos Naturais , Sistemas de Informação Geográfica , Humanos
20.
Zhonghua Er Ke Za Zhi ; 59(4): 280-285, 2021 Apr 02.
Artigo em Chinês | MEDLINE | ID: mdl-33775046

RESUMO

Objective: To explore the underlying genetic causes of neonatal encephalopathy complicated with perinatal asphyxia. Methods: From the neonates recruited to the Neonatal Genome Project of Children's Hospital of Fudan University between January 2016 and January 2019, 113 neonates with neonatal encephalopathy and acute peripartum or intrapartum event or Apgar score ≤7 were enrolled in this study. The clinical data, laboratory results, the findings of electroencephalograph and magnetic resonance imaging or head ultrasound, and the genetic information were retrospectively analyzed. Results: Of the 133 neonates with neonatal encephalopathy and acute peripartum or intrapartum event or Apgar score ≤7 scores, 77 (57.9%) were males, 56 (42.1%) were female, 56 (42.1%) were delivered via cesarean section, and 77(57.9%) were born by vaginal delivery. Among these cases, 68 (51.1%) were diagnosed of hypoxic ischemic encephalopathy, 25 (18.8%) had intracranial hemorrhage, 20 (15%) were related to genetic diseases, and 5 (3.8%) had sepsis without central nervous infection. A total of 20 cases with positive results by next-generation sequencing test were identified, including 19 cases with pathogenic variations and 1 case with variation of uncertain significance. These 20 cases included 4 cases with congenital myopathy (2 cases of MTM1 gene pathogenic variants, 1 case of ACTA1 and 1 case of RYR1 gene pathogenic variants), 4 cases with genetic syndrome (2 cases of CHD7 gene pathogenic variants, 1 case of PTN11 gene pathogenic variant, and 1 case of NSDHL gene pathogenic variant), 3 cases with metabolic disorders (1 case of OTC gene pathogenic variant, 1 case of MTHFR gene pathogenic variant, and 1 case of ALDH7A1 gene pathogenic variant), 2 cases with epileptic encephalopathy (1 case of KCNT1 and 1 case of PACS2 gene pathogenic variants), 1 case with congenital central hypoventilation syndrome (PHOX2B gene pathogenic variant) and 6 cases with copy-number pathogenic variations. Among these 20 cases, 8(40.0%) neonates were presented with persistent hypotonia, 7(35.0%) neonates with seizures, and 5(25.0%) neonates with congenital malformation. Genetic counseling and further follow-up were performed or suggested for these 20 cases; 4 neonates were deceased, 10 neonates underwent palliative care, and 6 neonates were improved after supportive care and their further follow-up plan were performed in clinics. Conclusions: Genetic diseases are not rare in neonates with neonatal encephalopathy complicated with perinatal hypoxia event. The common causes in these neonates include congenital myopathy, metabolic disorders, genetic syndrome, and epilepsy encephalopathy.


Assuntos
Cesárea , Hipóxia-Isquemia Encefálica , 3-Hidroxiesteroide Desidrogenases , Criança , Feminino , Genótipo , Humanos , Hipóxia , Hipóxia-Isquemia Encefálica/genética , Recém-Nascido , Masculino , Proteínas do Tecido Nervoso , Fenótipo , Canais de Potássio Ativados por Sódio , Gravidez , Estudos Retrospectivos
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