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2.
Food Res Int ; 129: 108802, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32036926

RESUMO

Phenolic acids are added to some dairy products as functional ingredients. The molecular interactions between the phenolic acids and milk proteins impacts their functional performance and product quality. In this study, the interactions between a milk protein (ß-casein) and a number of phenolic acids was investigated: 3,4-dihydroxybenzoic acid (DA); gallic acid (GA); syringic acid (SA); caffeic acid (CaA); ferulic acid (FA); and, chlorogenic acid (ChA). The structural characteristics of the phenolic acids, such as type, hydroxylation, methylation, and steric hindrance, affected their binding affinity to ß-casein. The strength of the binding constant decreased in the following order: CaA > ChA > FA > SA > GA > DA. Cinnamic acid derivatives (CaA, FA, and ChA) exhibited a stronger binding affinity with ß-casein than benzoic acid derivatives (DA, GA, and SA). Hydrophobic forces and electrostatic interactions dominated the interactions of ß-casein with benzoic acid and cinnamic acid derivatives, respectively. The number of hydroxyl groups on the phenolic acids enhanced their binding ability, while steric hindrance effects reduced their binding ability. The influence of methylation depended on phenolic acid type. After binding with phenolic acids, the conformation of the ß-casein changed, with a loss of random coil structure, an increase in α-helix structure, and a decrease in surface hydrophobicity. Furthermore, the presence of ß-casein decreased the in vitro antioxidant capacities of the phenolic acids, especially for gallic acid. These findings provide some useful insights into the structure-activity relationships of the interaction between ß-casein and phenolic acids.

3.
Molecules ; 25(3)2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32050515

RESUMO

Although the effects of melatonin on plant abiotic and biotic stress resistance have been explored in recent decades, the accumulation of endogenous melatonin in plants and its influence on fruit quality remains unclear. In the present study, melatonin accumulation levels and the expression profiles of five synthesis genes were investigated during fruit and leaf development in sweet cherry (Prunus avium L.). Melatonin was strongly accumulated in young fruits and leaves, then decreased steadily with maturation. Transcript levels of PacTDC and PacSNAT were highly correlated with melatonin content in both fruit and leaves, indicating their importance in melatonin accumulation. Furthermore, application of 50 and 100 µmol·L-1 of melatonin to leaves had a greater influence on fruit quality than treatments applied to fruits, by significantly improving fruit weight, soluble solids content, and phenolic content including total phenols, flavanols, total anthocyanins, and ascorbic acid. Meanwhile, melatonin application promoted the antioxidant capacity of fruit assayed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylben zothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP). These results provide insights into the physiological and molecular mechanisms underlying melatonin metabolism of sweet cherry.

4.
Rev Sci Instrum ; 91(1): 014703, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32012643

RESUMO

A pulsed intense magnetic field device was developed for the Shanghai Shenguang-II (SG-II) laser facility. The device using a double-turn coil with 12 mm diameter is capable of producing a peak current of 42 kA with 280 ns rising edge and 200 ns flat top width. A peak magnetic field of 8.8 T is achieved at the center of the coil. A two-section transmission line composed by a flexible section and a rigid section is designed to meet the target chamber environment of SG-II laser facility. The flexible section realizes the soft-connection between the capacitor bank and the target chamber, which facilitates the installation of the magnetic field device and the adjustment of the coil. The rigid section is as small as possible so that it can be inserted into the target chamber from any smallest flange, realizing elastic magnetic field configuration. The magnetic coil inside the chamber can be adjusted finely through a mechanical component on the rigid transmission line outside the target chamber. The adjustment range is up to 5 cm in both radial and axial directions with ∼50 µm precision. The device has been successfully operated on SG-II laser facility.

5.
Langmuir ; 2020 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-32013443

RESUMO

Marine biofouling profoundly influences marine industries and activities. It slows the speed and increases the fuel consumption of ships, corrodes the offshore platforms and blocks the seawater pipelines. The most effective and economic antifouling approach is using coatings. Fouling release coatings (FRCs) with low surface free energy and high elasticity weakly adhere to marine organisms so they can be readily removed by the water shear force. FRCs have received increasing interest because they are biocide-free and hence eco-friendly. Yet, traditional silicone based FRCs have weak adhesion to substrate, low mechanical strength and low fouling resistance, limiting their applications. In recent years, many attempts have been made to improve their mechanical properties and fouling resistance. The review deals with the progress in construction of high-performance silicone based fouling release surfaces.

6.
Mikrochim Acta ; 187(2): 145, 2020 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-31970521

RESUMO

The authors describe a novel electrochemical determination method for bisphenol A (BPA) based on the electrosynthesised Cu-BTC (H3BTC: trimesic acid) films. Using H3BTC as the ligand, Cu(NO3)2 as the precursor of copper ions, and triethylamine hydrochloride (Et3NHCl) as the probase source, Cu-BTC films were directly deposited on glassy carbon electrode (GCE) surface via cathodic electrochemical reduction under -1.30 V. Considering the electrocatalytic activity of metal center Cu2+, Cu-BTC films were applied to construct the electrochemical determination platform for BPA. Chronocoulometry and electrochemical impedance spectroscopy were used to study the signal enhancement mechanism. The determination conditions were optimized. As a result, a sensitive electrochemical method was constructed for BPA. The peak currents, best measured at voltage of 0.496 V vs. SCE (KCl saturated calomel reference electrode), increase linearly in the range from 5.0 to 2000 nM. The value of determination limit is 0.72 nM. The proposed method was successfully applied to determine BPA in spiked urine, spiked waste water samples and plastic products. The results were in good agreement with those obtained for the same samples by high-performance liquid chromatography (HPLC). Graphical abstract Schematics for the construction of electrochemical determination for bisphenol A.

7.
Med Phys ; 2020 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-31954078

RESUMO

PURPOSE: Increased utilization of MRI in radiotherapy has caused a growing need for phantoms that provide tissue-like contrast in both CT and MRI images. Such phantoms can be used to compare MRI-based processes with CT-based clinical standards. Here, we develop and demonstrate the clinical utility of a 3D-printed anthropomorphic pelvis phantom containing materials capable of T1 , T2 and electron density matching for a clinically relevant set of soft tissues and bone. METHODS: The phantom design was based on a male pelvic anatomy template with thin boundaries separating tissue types. Slots were included to allow insertion of various dosimeters. The phantom structure was created using a 3D-printer. The tissue compartments were filled with carrageenan-based materials designed to match the T1 and T2 relaxation times and electron densities of the corresponding tissues. CT and MRI images of the phantom were acquired and used to compare phantom T1 and T2 relaxation times and electron densities to literature-reported values for human tissue. To demonstrate clinical utility, the phantom was used for end-to-end testing of an MRI-only treatment simulation and planning workflow. Based on a T2 -weighted MRI image, synthetic CTs (sCTs) were created using a statistical decomposition algorithm (MRIPlanner, Spectronic Research AB, Sweden) and used for dose calculation during treatment planning of VMAT and 7-field IMRT prostate plans. The plans were delivered on a Truebeam STX (Varian Medical Systems, Palo Alto, CA), with film and a 0.3 cc ion chamber used to measure the delivered dose. Doses calculated on the CT and sCTs were compared using common dose volume histogram (DVH) metrics. RESULTS: T1 and T2 relaxation time and electron density measurements for muscle, prostate and bone agreed well with literature-reported in vivo measurements. Film analysis resulted in a 99.7% gamma-pass-rate (3.0%, 3.0mm) for both plans. The ion chamber-measured dose discrepancies at the isocenter were 0.36% and 1.67% for the IMRT and VMAT plans respectively. The differences in PTV D98% and D95% between plans calculated on the CT and 1.5T/3.0T-derived sCT images were under 3%. CONCLUSION: The developed phantom provides tissue-like contrast on MRI and CT and can be used to validate MRI-based processes through comparison with standard CT-based processes.

8.
Artigo em Inglês | MEDLINE | ID: mdl-31942788

RESUMO

We report an efficient, reusable and selective 6-Aminothiouracil (ATA) modified Zr(IV)-based adsorbent (defined as UiO-66-ATA(Zr)) for Lead ion removal in water. The adsorption equilibrium time and the maximum sorption capacity of UiO-66-ATA(Zr) for Pb(II) are respectively 120 min and 386.98 mg/g at pH 4 and 298 K. The Pb(II) removal rate reaches 96% at 60 min and exceeds 99% at the equilibrium state in the pH range of 2.0-5.8. Hill and Pseudo-second-order models can well describe the sorption process. Pb(II) adsorbing onto UiO-66-ATA(Zr) is irreversible, favorable chemisorption with multi-molecule participation and film diffusion control. The calculations of DFT, the experimental results and the characterization analyses suggest that the binding mechanisms are the chelation, ion exchange and electrostatic interactions between hydroxyl/amino/sulfhydryl groups of UiO-66-ATA(Zr) and Pb(II). Besides, UiO-66-ATA(Zr) has a better affinity to Pb(II) than the coexisting ions in water and an excellent repeatability at eight cycles of adsorption. Moreover, the thermodynamic study shows that UiO-66-ATA(Zr) adsorbing Pb(II) is an endothermic reaction. Thus UiO-66-ATA(Zr) is a prospective sorbent for Pb(II) removal under the initiative of environmental protection and water purification, and this work may also provide an idea for industrial catalysis.

9.
World Neurosurg ; 133: e320-e326, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31520758

RESUMO

BACKGROUND: Compared with intracranial aneurysms (IAs) at other locations, pericallosal artery aneurysms (PAAs) have demonstrated an extremely high risk of rupture. However, owing to their rarity, our understanding of their morphological characteristics has been limited, and whether the morphological characteristics of PAAs contribute to this high rupture risk has remained unexplored. In the present study, we aimed to provide a detailed description of the morphological characteristics of PAAs and investigate the association between its morphology and rupture risk compared with anterior circulation IAs at other locations. METHODS: A total of 40 patients with 45 PAAs and 348 patients with 392 anterior circulation IAs at other locations were recruited. The clinical and radiological data for these patients were retrospectively reviewed. The differences in the morphological parameters, including the aneurysm diameter, neck width, height, width, parent artery diameter, inflow angle, aspect ratio (AR), size ratio (SR), and aneurysm diameter/width ratio, between PAAs and other IA groups were compared. RESULTS: Of the 45 PAAs, 22 (48.9%) had ruptured. The proportion of ruptured aneurysms was greater for PAAs than for anterior circulation IAs at other locations. For both ruptured and unruptured anterior circulation IAs, PAAs had the highest AR and SR among all IA groups and had the largest inflow angle. CONCLUSION: The morphological characteristics of PAAs are unique. Compared with other anterior circulation IAs, PAAs have significantly increased ARs, SRs, and inflow angles, which, ultimately, promote their high propensity toward rupture.

10.
J Infect Public Health ; 13(1): 110-117, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31337540

RESUMO

BACKGROUND: Although the specific etiology of Henoch-Schonlein purpura (HSP) is still unknown, several kinds of infectious triggers have been proved to participate in its pathogenesis. The objectives of present study were to analyze the association of the infectious triggers with childhood HSP in Anhui province, China. METHODS: 1200 HSP children were recruited from January 2015 to December 2017. Serum antistreptolysin O titer, TORCH, Epstein-Barr virus, helicobacter pylori (HP), Mycoplasma antibodies (MP-Ab), tubercle bacillus antibody (TB-Ab), respiratory pathogens (legionella pneumophila, chlamydia pneumoniae, adenovirus, respiratory syncytial virus, influenza A virus, influenza B virus, rickettsia, parainfluenza virus) were determined. Patients' histories were obtained by interviews and questionnaires. RESULTS: The annual incidence of HSP was 8.13-9.17 per 100,000. HSP occurred more commonly in spring and winter than in summer with an obvious west-to-east gradient. On admission, several potential infections were identified in 611 cases (50.92%). The infectious agents including streptococcus, HP, MP, parainfluenza, respiratory syncytial virus, TB and toxoplasma gondii were identified in 205 cases (17.08%), 71 cases (5.92%), 58 cases (4.83%), 6 cases (0.5%), 1 case (0.08%), 1 case (0.08%) and 1 case (0.08%) respectively. 123 cases (10.25%) relapsed or recurred more than one time; the mean number was 2.92, and the mean interval was 11.4 weeks. The infection was the most frequent trigger regardless of clinical phenotypes and relapse/recurrence. Symptomatic treatment plus adjunctive anti-infectious agents could significantly improve the remission rate of purpura in the infectious cases (x2=24.60, p<0.01). CONCLUSIONS: Streptococcus is the most frequent infectious agent in HSP children regardless of clinical phenotype or relapse/recurrence. The complete elimination of infectious triggers may help relieve cutaneous purpura.

11.
Bioresour Technol ; 296: 122337, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31727559

RESUMO

The shortage of food based feedstocks is a challenge in industrial biomanufacturing. In this study, genetically modified Escherichia coli strains were used to produce isopropanol as the mainly product from acetate, a cost-effective nonfood-based substrate. The isopropanol biosynthesis pathway was constructed by combining genes from Clostridium acetobutylicum (thlA, adc), E. coli (atoDA) and Clostridium beijerinckii (adh). E. coli MG1655 harboring the isopropanol biosynthesis pathway successfully produced isopropanol and low amounts of acetone from pure acetate. The enhancement of the acetate assimilation pathway coupled with cofactor engineering strategy further improved the production of isopropanol to 18.5 mM with a yield of 0.26 mol/mol. With simple treatment, two kinds of biologically produced acetate were utilized to generate 16.7 and 24.5 mM isopropanol with yields of 0.25 and 0.56 mol/mol, respectively. Engineered E. coli with an optimized isopropanol biosynthesis pathway can efficiently utilize biologically produced acetate to synthesize isopropanol.


Assuntos
2-Propanol , Clostridium acetobutylicum , Acetatos , Escherichia coli , Fermentação , Engenharia Metabólica
12.
Phys Med Biol ; 65(3): 035015, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31881546

RESUMO

To objectively compare the suitability of MRI pulse sequences and commercially available fiducial markers (FMs) for MRI-only prostate radiotherapy simulation. Most FMs appear as small signal voids in MRI images making them difficult to differentiate from tissue heterogeneities such as calcifications. In this study we use quantitative metrics to objectively evaluate the visibility of FMs in 27 patients and an anthropomorphic phantom with a variety of standard clinical MRI pulse sequences and commercially available FMs. FM visibility was quantified using the local contrast-to-noise-ratio (lCNR), the difference between the 80th and 20th percentile iso-intensity FM volumes (V fall) and the largest iso-intensity volume that can be distinguished from background: apparent-marker-volume (AMV). A larger lCNR and AMV, and smaller V fall represents a more easily identifiable FM. The number of non-marker objects visualized by each pulse sequence was calculated using FM-derived template-matching. The FM-based target-registration-error (TRE) between each MRI and the planning-CT image was calculated. Fiducial marker visibility was rated by two medical physicists with over three years of experience examining MRI-only prostate simulation images. The rater's classification accuracy was quantified using the F 1 score, which is the harmonic mean of the rater's precision and recall. These quantitative metrics and human observer ratings were used to evaluate FM identifiability in images from nine subtypes of T 1-weighted, T 2-weighted and gradient echo (GRE) pulse sequences in a 27-patient study. A phantom study was conducted to quantify the visibility of 8 commercially available FMs. In the patient study, the largest mean lCNR and AMV and, smallest normalized V fall were produced by the 3.0 T multiple-echo GRE pulse sequence (T 1-VIBE, 2° flip angle, 1.23 ms and 2.45 ms echo-times). This pulse sequence produced no false marker detections and TREs less than 2 mm in the left-right, anterior-posterior and cranial-caudal directions, respectively. Human observers rated the 1.23 ms echo-time GRE images with the best average marker visibility score of 100% and an F 1 score of 1. In the phantom study, the Gold-Anchor GA-200X-20-B (deployed in a folded configuration) produced the largest sequence averaged lCNR and AMV measurements at 16.1 and 16.7 mm3, respectively. Using quantitative visibility and distinguishability metrics and human observer ratings, the patient study demonstrated that multiple-echo GRE images produced the best gold FM visibility and distinguishability. The phantom study demonstrated that markers manufactured from platinum or iron-doped gold quantitatively produced superior visibility compared to their pure gold counterparts.

13.
Sci Total Environ ; 706: 136029, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31855629

RESUMO

The gas/particle (G/P) partitioning (KP) behavior is an important factor for the environmental fate of PAHs in atmosphere. Based on large database of log KP, equilibrium-state and steady-state models were applied for the comprehensive study with the G/P partitioning of PAHs, including the Harner-Bidleman (H-B) model, the Dachs-Eisenreich (D-E) model, and the Li-Ma-Yang (L-M-Y) model. For different sites, the trend of regression between log KP and log KOA was same, however, the slopes and intercepts were different. No obvious difference was observed between northern Chinese cities and southern Chinese cities. For congeners and aromatic rings of PAHs, the difference was much more obvious for the regressions, slopes and intercepts. The prediction of the D-E model and the H-B model matched well for the regression of the 4-rings and 5-rings PAHs, with >80% of monitoring data points in the range of ±1 log unit. The L-M-Y model only predicted well with the measurement for 4-rings PAHs with special values of log KOA. For different ranges of log KOA, the difference with the regression between log KP and log KOA was also obvious. Compared with our measurement, if 1 order of magnitude difference with log KP values between prediction and measurement was considered, the H-B model, the D-E model and the L-M-Y model can be only used when the log KOA in the ranges from 7.65 to 13.7, 6.88 to 13.5, and 7.65 to 11.7, respectively. Therefore, further studies with prediction models should be conducted for the G/P partitioning of PAHs. The results of this study provided new insights into the research field of the G/P partitioning of SVOCs.

14.
J Cell Physiol ; 235(1): 563-572, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31318050

RESUMO

Sepsis is a severe clinical disease, which is resulted from the excessive host inflammation response to the infection. Growing evidence indicates that Staphylococcus aureus pneumonia is a significant cause of sepsis, which can lead to intestinal injury, inflammation, and apoptosis. Studies have shown that miR-182-5p can serve as a tumor oncogene or a tumor suppressive microRNA in various cancers, however, its biological role in sepsis is still uninvestigated. Here, we reported that miR-182-5p was obviously increased in S. aureus pneumonia mice models. Loss of miR-182-5p inhibited intestinal damage and intestinal apoptosis as indicated by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In addition, we observed the lack of miR-182-5p altered the local inflammatory response to pneumonia in the intestine. Elevated tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were observed in intestinal tissue of pneumonia groups compared with the shams. Furthermore, miR-182-5p knockout (KO) pneumonia group demonstrated decreased levels of intestinal TNF-α and IL-6. Primary murine intestinal epithelial cells were isolated and cultured in our investigation. We exhibited downregulation of miR-182-5p repressed intestinal epithelial cells apoptosis and rescued the cell viability. Meanwhile, miR-182-5p caused elevated cell apoptosis and reduced cell proliferation. Moreover, the surfactant protein D (SP-D) binds with the bacterial pathogens and remove the pathogens and apoptotic bodies, which exhibits important roles in modulating immune responses. It was displayed in our study that SP-D was greatly decreased in pneumonia mice models. SP-D was predicted as a downstream target of miR-182-5p. These data concluded that miR-182-5p promoted intestinal injury in S. aureus pneumonia-induced sepsis via targeting SP-D.

15.
Chronobiol Int ; : 1-14, 2019 Dec 06.
Artigo em Inglês | MEDLINE | ID: mdl-31809585

RESUMO

In aquaculture, it is necessary to determine of the diurnal biological variations in the intestines to determine an appropriate feeding schedule. The present study aimed to examine the transcriptomes of the Pelteobagrus vachellii intestines at four time points (0 h, 6 h, 12 h, and 18 h) within a light/dark cycle. In comparison with the zeitgeber time 0 (ZT0) transcriptomes, we identified 37,842 unigenes with significant differential expression, including 6,638; 9,626; and 7,938 that genes upregulated, and 3,507; 4,703; and 5,412 genes that were down regulated at 4, 12, and 24 h respectively. The differentially expressed unigenes were subjected to enrichment analysis, which indicated the involvement of the major digestive pathways, including digestion of protein, lipid and carbohydrate, catabolic process (protein, carbohydrate and lipid), and circadian rhythm. We selected 73 key differentially expressed genes (DEGs) from among these pathways and identified DEGs that showed increased expression at night, including those encoding trypsin-3, chymotrypsinogen 2, amino acid transporter, maltase-glucoamylase, facilitated glucose transporter, lipase, phospholipase, fatty acid-binding protein, fatty acid synthase, long-chain fatty acid transport protein, and apolipoprotein. Moreover, DEGs involved of circadian rhythm were identified, including brain-muscle-Arnt-like 1 (BMAL1), cryptochrome-1, circadian locomoter output cycles protein kaput (CLOCK) and period circadian protein homolog 1-3. Finally, the expression levels of 12 unigenes were analyzed using quantitative real-time PCR, which were in accordance with RNA-sequencing analysis. In general, the expression of genes related to the digestion of proteins, lipids, and carbohydrates showed upregulated expression at night; however, the peak time of expression of transporters for different nutrition molecules showed more diversification within the light/dark cycle.

16.
BMC Genomics ; 20(Suppl 13): 928, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31881833

RESUMO

BACKGROUND: Identification of protein-protein interactions (PPIs) is crucial for understanding biological processes and investigating the cellular functions of genes. Self-interacting proteins (SIPs) are those in which more than two identical proteins can interact with each other and they are the specific type of PPIs. More and more researchers draw attention to the SIPs detection, and several prediction model have been proposed, but there are still some problems. Hence, there is an urgent need to explore a efficient computational model for SIPs prediction. RESULTS: In this study, we developed an effective model to predict SIPs, called RP-FIRF, which merges the Random Projection (RP) classifier and Finite Impulse Response Filter (FIRF) together. More specifically, each protein sequence was firstly transformed into the Position Specific Scoring Matrix (PSSM) by exploiting Position Specific Iterated BLAST (PSI-BLAST). Then, to effectively extract the discriminary SIPs feature to improve the performance of SIPs prediction, a FIRF method was used on PSSM. The R'classifier was proposed to execute the classification and predict novel SIPs. We evaluated the performance of the proposed RP-FIRF model and compared it with the state-of-the-art support vector machine (SVM) on human and yeast datasets, respectively. The proposed model can achieve high average accuracies of 97.89 and 97.35% using five-fold cross-validation. To further evaluate the high performance of the proposed method, we also compared it with other six exiting methods, the experimental results demonstrated that the capacity of our model surpass that of the other previous approaches. CONCLUSION: Experimental results show that self-interacting proteins are accurately well-predicted by the proposed model on human and yeast datasets, respectively. It fully show that the proposed model can predict the SIPs effectively and sufficiently. Thus, RP-FIRF model is an automatic decision support method which should provide useful insights into the recognition of SIPs.

17.
Artigo em Inglês | MEDLINE | ID: mdl-31681739

RESUMO

Background: Mesothelioma is a rare and aggressive tumor. Bone metastasis often occurs in the later stages of this disease along with poor quality of life. Thus, it is important to explore the tumorigenesis and bone metastasis mechanism of invasive mesothelioma. For this purpose, we established two nomograms based on tumor-infiltrating immune cells and ceRNA networks to describe the molecular immunity and the clinical prediction of mesothelioma patients with bone metastasis. Method: The expression profiles of mRNAs, lncRNAs, and miRNAs of 87 primary mesotheliomas were obtained from the TCGA database; there were four patients with bone metastasis and 83 patients without. We constructed a ceRNAs network based on the differentially expressed RNAs between mesothelioma and bone metastasis. CIBERSORT was used to distinguish 22 immune cell types from the tumor transcriptomes. Kaplan-Meier survival analysis and the Cox proportional hazards model were used to evaluate the prognostic value of each factor. Prognosis-associated immune cells and ceRNAs were applied to establish prediction nomograms. The receiver operating characteristic curves (ROC) and calibration curves were utilized to assess the discrimination and accuracy of the nomogram. Results: Differential analysis revealed that 20 lncRNAs, 15 miRNAs, and 230 mRNAs were significantly different in mesothelioma samples vs. bone metastasis samples. We constructed the ceRNA network to include 10 protein-coding mRNAs, 8 lncRNAs, and 10 miRNAs. Nine of 28 ceRNAs were found to be significant in the Kaplan-Meier analysis. Out of the 22 cell types, the fraction of dendritic cells resting (P = 0.018) was significantly different between the bone metastasis group and the non-bone metastasis group. The ROC and the calibration curves, based on ceRNA networks and tumor-infiltrating immune cells, respectively, suggested acceptable accuracy (AUC of 3-year survival: 0.827, AUC of 5-year survival: 0.840; AUC of 3-year survival: 0.730; AUC of 5-year survival: 0.753). Notably, based on the co-expression patterns between ceRNAs and Immune cells, we found that the hsa-miR-582-5p, CASP9, dendritic cells resting, ANIX2, T cells CD8, and T cells CD4 memory resting might be associated with the mesothelioma bone metastasis. Conclusion: Based on ceRNA networks and patterns of immune infiltration, our study provided a valid bioinformatics basis in order to explore the molecular mechanism and predict the possibility of mesothelioma bone metastasis.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31681747

RESUMO

Background: Kidney renal clear cell carcinoma (KIRC) is the malignancy originated from the renal epithelium, with a high rate of distant metastasis. Aberrant alternative splicing (AS) of pre-mRNA are widely reported to be involved in the tumorigenesis and metastasis of multiple cancers. The aim of this study is to explore the mechanism of alternative splicing events (ASEs) underlying tumorigenesis and metastasis of KIRC. Methods: RNA-seq of 537 KIRC samples downloaded from the TCGA database and ASEs data from the TCGASpliceSeq database were used to identify ASEs in patients with KIRC. The univariate and Lasso regression analysis were used to screen the most significant overall survival-related ASEs (OS-SEs). Based on those, the OS-SEs model was proposed. The interaction network of OS-SEs and splicing factors (SFs) with absolute value of correlation coefficient value >0.750 was constructed by Pearson correlation analysis. The OS-SEs significantly related to distant metastasis and clinical stage were identified by non-parametric test, and those were also integrated into co-expression analysis with prognosis-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways identified by Gene Set Variation Analysis (GSVA). ASEs with significance were selected for multiple online database validation. Results: A total of prognostic 6,081 overall survival-related ASEs (OS-SEs) were identified by univariate Cox regression analysis and a prediction model was constructed based on 5 OS-SEs screened by Lasso regression with the Area Under Curve of 0.788. Its risk score was also illustrated to be an independent predictor, which the good reliability of the model. Among 390 identified candidate SFs, DExD-Box Helicase 39B (DDX39B) was significantly correlated with OS and metastasis. After external database validation, Retained Intron of Ras Homolog Family Member T2 (RHOT2) and T-Cell Immune Regulator 1 (TCIRG1) were identified. In the co-expression analysis, overlapped co-expression signal pathways for RHOT2 and TCIRG1 were sphingolipid metabolism and N-glycan biosynthesis. Conclusions: Based on the results of comprehensive bioinformatic analysis, we proposed that aberrant DDX39B regulated RHOT2-32938-RI and TCIRG1-17288-RI might be associated with the tumorigenesis, metastasis, and poor prognosis of KIRC via sphingolipid metabolism or N-glycan biosynthesis pathway.

19.
BMC Infect Dis ; 19(1): 924, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31666025

RESUMO

BACKGROUNDS: Pulmonary tuberculosis (PTB) is a major health and economic burden. Accurate PTB detection is an important step to eliminating TB globally. Interferon gamma-induced protein 10 (IP-10) has been reported as a potential diagnostic marker for PTB since 2007. In this study, a meta-analysis approach was used to assess diagnostic value of IP-10 for PTB. METHODS: Web of Science, PubMed, the Cochrane Library, and Embase databases were searched for studies published in English up to February 2019. The pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), the area under the curve (AUC) and hierarchical summary receiver operating characteristic (HSROC) curve were estimated by the HSROC model and random effect model. RESULTS: Eighteen studies including 2836 total participants met our inclusion criteria. The pooled sensitivity, specificity, PLR, and NLR of IP-10 for PTB detection were 86, 88%, 7.00, and 0.16, respectively. The pooled DOR was 43.01, indicating a very powerful discriminatory ability of IP-10. The AUC was 0.93 (95% CI: 0.91-0.95), showed the accuracy of IP-10 was good. Meta-regression showed that there was no heterogeneity with respect to TB burden, study design type, age, IP-10 assay method, IP-10 condition and HIV-infection status. CONCLUSIONS: Our results showed that IP-10 is a promising marker for differentiating PTB from non-TB.


Assuntos
Biomarcadores/sangue , Quimiocina CXCL10/sangue , Tuberculose Pulmonar/sangue , Área Sob a Curva , Ensaios Clínicos como Assunto , Humanos , Razão de Chances , Curva ROC , Sensibilidade e Especificidade , Tuberculose Pulmonar/diagnóstico
20.
Quant Imaging Med Surg ; 9(9): 1516-1527, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31667138

RESUMO

Background: To review and evaluate approaches to convolutional neural network (CNN) reconstruction for accelerated cardiac MR imaging in the real clinical context. Methods: Two CNN architectures, Unet and residual network (Resnet) were evaluated using quantitative and qualitative assessment by radiologist. Four different loss functions were also considered: pixel-wise (L1 and L2), patch-wise structural dissimilarity (Dssim) and feature-wise (perceptual loss). The networks were evaluated using retrospectively and prospectively under-sampled cardiac MR data. Results: Based on our assessments, we find that Resnet and Unet achieve similar image quality but that former requires only 100,000 parameters compared to 1.3 million parameters for the latter. The perceptual loss function performed significantly better than L1, L2 or Dssim loss functions as determined by the radiologist scores. Conclusions: CNN image reconstruction using Resnet yields comparable image quality to Unet with 10X the number of parameters. This has implications for training with significantly lower data requirements. Network training using the perceptual loss function was found to better agree with radiologist scoring compared to L1, L2 or Dssim loss functions.

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