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1.
Sci Rep ; 11(1): 2083, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33483566

RESUMO

To describle how respiratory tract infections (RTIs) that occurred in children with allergic asthma (AA) on allergen immunotherapy (AIT) during an influenza season. Data including clinical symptoms and treatment history of children (those with AA on AIT and their siblings under 14 years old), who suffered from RTIs during an influenza season (Dec 1st, 2019-Dec 31st, 2019), were collected (by face to face interview and medical records) and analyzed. Children on AIT were divided into 2 groups: stage 1 (dose increasing stage) and stage 2 (dose maintenance stage). Their siblings were enrolled as control. During the study period, 49 children with AA on AIT (33 patients in stage 1 and 16 patients in stage 2) as well as 49 children without AA ( their siblings ) were included. There were no significant differences in occurrences of RTIs among the three groups (p > 0.05). Compared with children in the other two groups, patients with RTIs in stage 2 had less duration of coughing and needed less medicine. Children on AIT with maintenance doses had fewer symptoms and recovered quickly when they were attacked by RTIs, which suggested that AIT with dose maintenance may enhance disease resistance of the body.

2.
Am Heart J ; 2021 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-33465369

RESUMO

BACKGROUND: Double kissing (DK) crush approach for patients with coronary bifurcation lesions, particularly localized at distal left main or lesions with increased complexity, is associated with significant reduction in clinical events when compared with provisional stenting (PS). Recently, randomized clinical trial has demonstrated the net clinical benefits by intravascular ultrasound (IVUS)-guided implantation of drug-eluting stent (DES) in all-comers. However, the improvement in clinical outcome after DK crush treatment guided by IVUS over angiography-guidance for patients with complex bifurcation lesions have never been studied in a randomized fashion. TRIAL DESIGN: DKCRUSH VIII study is a prospective, multicenter, randomized controlled trial designed to assess superiority of IVUS-guided versus angiography-guided DK crush stenting in patients with complex bifurcation lesions according to DEFINITION criteria. A total of 556 patients with complex bifurcation lesions will be randomly (1:1 of ratio) assigned to IVUS-guided or angiography-guided DK crush stenting group. The primary endpoint is the rate of 12-month target vessel failure (TVF), including cardiac death, target vessel myocardial infarction (TV-MI) or clinically driven target vessel revascularization (CD-TVR). The secondary endpoints consist of the individual component of primary endpoint, all-cause death, MI, and in-stent restenosis. The safety endpoint is the incidence of definite or probable stent thrombosis. An angiographic follow-up will be performed for all patients at 13 months and clinical follow-up will be continued annually until 3 years after the index procedure. CONCLUSION: DKCRUSH VIII trial is the first study designed to evaluate the differences in efficacy and safety between IVUS-guided and angiography-guided DK crush stenting in patients with complex true bifurcation lesions. This study will also provide IVUS-derived criteria to define optimal DK crush stenting for bifurcation lesions at higher complexity.

3.
Integr Zool ; 2021 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-33452844

RESUMO

Analysis of the intestinal microbiota and physiological parameters in mammalian infancy can reveal health status. In this study, we used a combination of molecular and immunochemical approaches to assess fecal microbiota as well as Cortisol (Cor), Triiodothyronine (T3), and immunoglobulin A (IgA) levels of young forest musk deer (FMD), from birth to one month after weaning (7 days of age-110 days of age). During development as the diet of FMD changes from consuming milk to eating plants, the richness and diversity of intestinal microbiota of young FMD increased significantly. Cor levels remained unchanged throughout early development while significantly increased after weaning, T3 and IgA initially were derived from milk during lactation, significantly decreased after weaning. Correlation network analysis showed that the community of food-oriented microbes were highly structured and that many genera were correlated. Overall, this study provides scientific insights into effective management strategies for the protection of FMD population.

4.
Sensors (Basel) ; 21(2)2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33477768

RESUMO

Deep squat, bench press and hard pull are important ways for people to improve their strength. The use of sensors to measure force is rare. Measuring strength with sensors is extremely valuable for people to master the intensity of exercise to scientifically effective exercise. To this end, in this paper, we used a real-time wireless motion capture and mechanical evaluation system of the wearable sensor to measure the dynamic characteristics of 30 young men performing deep squat, bench press and hard pull maneuvers. The data of tibia were simulated with AnyBody 5.2 and ANSYS 19.2 to verify the authenticity. The result demonstrated that the appropriate force of the deep squat elbow joint, the hip joint and the knee joint is 40% 1RM, the appropriate force of the bench press is 40% 1RM and the appropriate force of the hard pull is 80% 1RM. The external force is the main factor of bone change. The mechanical characteristics of knee joint can be simulated after the Finite Element Analysis and the simulation of AnyBody model are verified.

5.
BMC Genomics ; 22(1): 64, 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33468053

RESUMO

BACKGROUND: miRNAs play critical roles in growth and development. Various studies of chicken muscle development have focused on identifying miRNAs that are important for embryo or adult muscle development. However, little is known about the role of miRNAs in the whole muscle development process from embryonic to post-hatching periods. Here, we present a comprehensive investigation of miRNA transcriptomes at 12-day embryo (E12), E17, and day 1 (D1), D14, D56 and D98 post-hatching stages. RESULTS: We identified 337 differentially expressed miRNAs (DE-miRNAs) during muscle development. A Short Time-Series Expression Miner analysis identified two significantly different expression profiles. Profile 4 with downregulated pattern contained 106 DE-miRNAs, while profile 21 with upregulated pattern contained 44 DE-miRNAs. The DE-miRNAs with the upregulated pattern mainly played regulatory roles in cellular turnover, such as pyrimidine metabolism, DNA replication, and cell cycle, whereas DE-miRNAs with the downregulated pattern directly or indirectly contributed to protein turnover metabolism such as glycolysis/gluconeogenesis, pyruvate metabolism and biosynthesis of amino acids. CONCLUSIONS: The main functional miRNAs during chicken muscle development differ between embryonic and post-hatching stages. miRNAs with an upregulated pattern were mainly involved in cellular turnover, while miRNAs with a downregulated pattern mainly played a regulatory role in protein turnover metabolism. These findings enrich information about the regulatory mechanisms involved in muscle development at the miRNA expression level, and provide several candidates for future studies concerning miRNA-target function in regulation of chicken muscle development.

6.
Oncol Res ; 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33419488

RESUMO

This study was designed to investigate the precise mechanisms of miR-325-3p/S100A2 axis in breast cancer (BC). In this study, we found that the level of miR-325-3p was dramatically increased in BC tissues and cell lines, and the expression of S100A2 was significantly decreased. And the high level of miR-325-3p was closely associated with low expression of S100A2 in BC tissues. Moreover, introduction of miR-325-3p significantly promoted proliferation, invasion and EMT of BC cells. Bioinformatics analysis predicted that the S100A2 was a potential target gene of miR-325-3p. Luciferase reporter assay demonstrated that miR-325-3p could directly target S100A2. In addition, miR-325-3p overexpression had the similar effects with knockdown of S100A2 on BC cells. Overexpression of S100A2 in BC cells partially reversed the promoted effects of miR-325-3p mimic. Overexpression of miR-325-3p promoted cell proliferation, invasion and EMT of BC cells by directly down-regulating S100A2 expression.

7.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 35(1): 76-81, 2021 Jan 15.
Artigo em Chinês | MEDLINE | ID: mdl-33448203

RESUMO

Objective: To investigate the effectiveness of double internal fixations of clavicle and scapula and intraoperative reduction of glenopolar angle in the treatment of floating shoulder injuries. Methods: Between January 2010 and June 2019, 13 patients with floating shoulder injury were treated with double internal fixation of clavicle and scapula and intraoperative reduction of glenopolar angle. There were 11 males and 2 females with an average age of 48 years (range, 25-65 years). The causes of injury included falling from height in 2 cases, traffic accident injury in 3 cases, heavy object injury in 2 cases, and other injuries in 6 cases. There were 2 cases of Ⅰ-B-3 type, 1 case of Ⅰ-C-2 type, 1 case of Ⅰ-C-3 type, 3 cases of Ⅱ-B-2 type, 1 case of Ⅱ-B-3 type, 1 case of Ⅱ-B-4 type, 2 cases of Ⅱ-C-2 type, 1 case of Ⅱ-C-4 type, and 1 case of Ⅱ-D-3 type according to the classification of floating shoulder injury. All patients had unilateral clavicle fracture with scapular neck fracture, 1-4 superior shoulder suspensory complex (SSSC) injuries. The time from injury to operation was 7-17 days, with an average of 12 days. The glenopolar angle, subacromail space, anteroposterior inclination angle of scapular glenoid, scapular glenoid up and down angle were measured before and after operation; Constant-Murly score and Herscovici score were used to evaluate the recovery of shoulder joint function. Results: All incisions healed by first intention, and there was no early postoperative complications such as infection. All 13 cases were followed up 12-48 months, with an average of 25.2 months. Both the clavicle and the scapula had bone union, and the average healing time was 6 months and 4 months respectively. There were no complications such as nonunion, shoulder deformity, plate fracture or failure of internal fixation, acromion impingement syndrome, and frozen shoulder. At last follow-up, the glenopolar angle, subacromail space, anteroposterior inclination angle of scapular glenoid, and scapular glenoid up and down angle were all corrected significantly ( P<0.05). The pain, function, activity, muscle strength scores, and total score in Constant-Murly score were significantly improved when compared with preoperative scores ( P<0.05). According to the Herscovici scoring standard, the shoulder joint function was evaluated as excellent in 8 cases, good in 3 cases, and fair in 2 cases. The excellent and good rate was 84.6%. Conclusion: Double internal fixation of clavicle and scapula to stabilize SSSC and reduct glenopolar angle during operation is an effective method for treating the floating shoulder injury.


Assuntos
Fraturas Ósseas , Lesões do Ombro , Adulto , Idoso , Clavícula/cirurgia , Feminino , Fixação Interna de Fraturas , Fraturas Ósseas/diagnóstico por imagem , Fraturas Ósseas/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Escápula/cirurgia , Ombro , Resultado do Tratamento
9.
J Natl Cancer Inst ; 2021 Jan 19.
Artigo em Inglês | MEDLINE | ID: mdl-33464295

RESUMO

The suicide rate has steadily increased in the United States during the past 2 decades. Cancer patients have elevated suicide risk because of prevalent psychological distress, treatment side effects, and potentially uncontrolled pain. Efforts to promote psychosocial and palliative care may reduce this risk. Using the 1999-2018 Multiple Cause of Death database, we found a decreasing trend of cancer-related suicide during the past 2 decades with an average annual percentage change (AAPC) of age-adjusted suicide rates of -2.8% (95% confidence interval [CI] = -3.5% to -2.1%) in contrast to an increasing trend of overall suicide rate (AAPC = 1.7%, 95% CI = 1.5% to 1.8%). We also observed the largest declines in cancer-related suicide rates among high-risk populations including male, older age, and certain cancer types, suggesting an evolving role of psycho-oncology and palliative and hospice care during this period.

10.
Sci Rep ; 11(1): 507, 2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33436771

RESUMO

Environmental natural organic matters (NOMs) have great effects on the physicochemical properties of engineering nanoparticles, which may impact the transport of nanoparticles across plasma membrane and the cytotoxicity. Therefore, the kinetics, uptake pathway and mass of transporting into A549 cell membrane of silver nanoparticles (AgNPs) coated with citric acid (CA), tartaric acid (TA) and fulvic acid (FA) were investigated, respectively. CA, FA and TA enhanced the colloidal stability of AgNPs in culture medium and have greatly changed the surface plasmon resonance spectrum of AgNPs due to the absorption of CA, FA and TA on surface of AgNPs. Internalizing model showed that velocity of CA-, TA- and FA-nAg transporting into A549 cell were 5.82-, 1.69- and 0.29-fold higher than those of the control group, respectively. Intracellular mass of Ag was dependent on mass of AgNPs delivered to cell from suspension, which obeyed Logistic model and was affected by NOMs that CA- and TA-nAg showed a large promotion on intracellular mass of Ag. The lipid raft/caveolae-mediated endocytosis (LME) of A549 cell uptake of AgNPs were susceptible to CA, TA and FA that uptake of CA-, TA- and FA-nAg showed lower degree of dependent on LME than that of the control (uncoated AgNPs). Actin-involved uptake pathway and macropinocytosis would have less contribution to uptake of FA-nAg. Overall, transmembrane transport of NOMs-coated AgNPs differs greatly from that of the pristine AgNPs.

11.
ChemSusChem ; 2021 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-33411408

RESUMO

Photocatalytic reduction of CO2 into value-added chemical fuels is an appealing approach to address energy crisis and global warming. CsPbBr3 quantum dots (QDs) are good candidates for CO2 reduction because of their excellent photoelectric properties, including high molar extinction coefficient, low exciton binding energy, and defect tolerance. However, the pristine CsPbBr3 QDs generally have low photocatalytic performance mainly due to dominant charge recombination and lack of efficient catalytic sites for CO2 adsorption/activation. Herein, we report a new photocatalytic system, in which CsPbBr3 QDs are coupled with covalent triazine frameworks (CTFs) for visible-light-driven CO2 reduction. In this hybrid photocatalytic system, the robust triazine rings and periodical pore structures of CTFs promote the charge separation in CsPbBr3 and endow them with strong CO2 adsorption/activation capacity. The resulting photocatalytic system exhibits excellent photocatalytic activity towards CO2 reduction. This work presents a new photocatalytic system based on CTFs and perovskite QDs for visible-light-driven CO2 reduction, which highlights the potential of perovskite-based photocatalysts for solar fuel applications.

12.
Neurosurg Rev ; 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33389342

RESUMO

The use of prophylactic anticonvulsants among patients with subarachnoid hemorrhage (SAH) is controversial. We sought to assess the effectiveness of different durations of prophylactic antiepileptic drug (AED) use among SAH patients. We searched the MEDLINE, Embase, Cochrane, and ClinicalTrials.gov databases until March 1, 2020. Randomized controlled trials or observational studies comparing different durations or different drugs were selected. The primary outcome was poor clinical outcomes. The secondary outcome was in-hospital seizure. Bayesian network meta-analysis was also performed to indirectly compare the effectiveness of different prophylaxes. A total of 5 papers were included. Three studies with a total of 959 patients were included in the analysis of the primary outcome; the results showed that long-term exposure to prophylactic AEDs (more than 3 days) led to poor clinical outcomes (OR 1.55; 95% CI 1.01-2.39; p = 0.045). Four studies with 1024 patients were included in the analysis of the secondary outcome; the results showed no association between the duration of prophylactic AED use and the occurrence of in-hospital seizures (OR 0.62; 95% CI 0.18-2.15; p = 0.447). In the network meta-analysis, no significant difference was found among the four different prophylaxes. Our findings suggested that, when compared with the short-term use, the long-term use of prophylactic AEDs in SAH patients has a similar effect on in-hospital seizure prevention but is associated with poor clinical outcomes. However, these findings were based on a small number of available studies with obvious heterogeneity in study design and different prescription regimens. Further well-designed studies are warranted to elucidate these questions.

13.
Artigo em Inglês | MEDLINE | ID: mdl-33430202

RESUMO

The implementation of precursor management can improve safety performance of construction projects through effectively managing the correlations between construction accidents and their precursors. However, a system of comprehensive knowledge about what precursors mean within the context of construction safety is still lacking. This study aims to capture the nature of precursors in the construction industry and explore the process of a precursor event evolving into a construction accident to fill this gap. Based on 135 construction accident reports in China, this study adopts grounded theory to identify different types of accident precursors and explore their interactions with the development of the accident. An indicator system of precursors for construction accidents was developed, which included two major categories of precursors: behavioral factors and physical factors and five minor categories of precursors: individual behavior factors, organizational driving factors, objective physical factors, construction environmental factors, mechanical equipment factors. In addition, a precursor management strategy that includes the three stages of identification, response and effectiveness testing was established. The results of the study reveal the correlations between precursors and construction accidents, which can promote construction professionals' better understanding about precursors and improve their capabilities of managing precursors in practice.

14.
Drug Discov Ther ; 14(6): 304-312, 2021 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-33390570

RESUMO

Acute graft-versus-host disease (aGvHD) remains lethal as a life-threatening complication after allogeneic hematopoietic stem cell transplantation (HSCT). Inflammatory responses play an important role in aGvHD. 5-Aminolevulinic acid combined with sodium ferrous citrate (5-ALA/SFC) has been widely reported to have a major effect on the anti-inflammatory response; however, these effects in aGvHD models have never been reported. In this study, a murine aGvHD model was developed by transferring spleen cells from donor B6/N (H-2kb) mice into recipient B6D2F1 (H-2kb/d) mice. In addition to evaluating manifestations in aGvHD mice, we analyzed the serum ALT/AST levels, liver pathological changes, infiltrating cells and mRNA expression of inflammation-related cytokines and chemokines. 5-ALA/SFC treatment significantly ameliorated liver injury due to aGvHD and decreased the population of liver-infiltrating T cells, resulting in a reduced expression of pro-inflammatory cytokines and chemokines. Furthermore, the mRNA expression proliferator-activated receptor-γcoactivator (PGC-1α) was enhanced, which might explain why 5-ALA/SFC treatment downregulates inflammatory signaling pathways. Our results indicated that 5-ALA/SFC can ameliorate liver injury induced by aGvHD through the activation of PGC-1α and modulation of the liver mRNA expression of inflammatory-related cytokines and chemokines. This may be a novel strategy for treating this disease.

15.
Exp Eye Res ; 204: 108449, 2021 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-33465395

RESUMO

Somatostatin plays important roles in modulating neuronal functions by activating the five specific G-protein coupled receptors (sst1-sst5). Previous studies have demonstrated that sst5 were expressed in retinal ganglion cells (RGCs) and sst5 agonist attenuated the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid-induced retinal neurotoxicity. In this study, we investigated effects and underlying mechanisms of the sst5 agonist L-817,818 on RGC injury induced by elevated intraocular pressure (COH) in experimental glaucoma. Our results showed that intraperitoneal administration of L-817,818 significantly reduced RGC loss and decreased the number of terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL)-positive RGCs in COH retinas, suggesting that L-817,818 may attenuate RGC apoptosis. Consistently, in COH retinas with L-817,818 administration, both the down-regulated mRNA and protein levels of anti-apoptotic Bcl-2 and the up-regulated mRNA and protein levels of pro-apoptotic Bax were partially reversed. L-817,818 administration downregulated the expression of apoptosis-related proteins caspase-9 and caspase-3 in COH retinas. In addition, L-817,818 administration reduced the concentrations of reactive oxygen species/reactive nitrogen species and malondialdehyde, and ameliorated the functions of mitochondrial respiratory chain complex (MRCC). Our results imply that administration of the sst5 agonist L-817,818 reduces RGC loss in COH rats through decreasing RGC apoptosis, which is mediated by regulating Bcl-2/Bax balance, reducing oxidative stress and rescuing activities of MRCC. Activation of sst5 may provide neuroprotective roles for RGCs in glaucoma.

16.
Talanta ; 222: 121425, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33167195

RESUMO

Folate receptors (FRs) are a class of valuable therapeutic target which is highly expressed on a variety of cancers. The accurate detection of the expression of FRs in different cells is conducive to improve the accuracy of FR targeted tumor therapy. Herein, a method based on nonimmobilized cell capillary electrophoresis (NICCE) combined with a mathematic model to quantify FRs on each single tumor cell was developed. At first, we studied the interactions between FA and A549, HT-29, HepG2, and U87MG cells by NICCE respectively, and calculated the kinetic parameters (Ka, k', ka, and kd). Next, we established a mathematic model to accurately determine the number of moles of FRs on per A549, HT-29, HepG2, and U87MG cell for the first time, that were (10.44 ± 0.53) × 10-19 mol, (34.32 ± 1.33) × 10-19 mol, (337.14 ± 10.11) × 10-19 mol, and (37.31 ± 2.13) × 10-19 mol. Then, these re-sults were proved to be consistent with the results of enzyme-linked immunosorbent assay (ELISA). Therefore, this method is simple, rapid, sensitive, and without protein separation or purification, which is expected to achieve clinical detection of cell membrane receptor expression level of cell membrane receptors on a single cell, which may be greatly beneficial to further clinical diagnosis and therapy.

17.
J Hazard Mater ; 403: 123540, 2021 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-33264846

RESUMO

Pristine biochar (CN600), ball-milled biochar (CN600-BM), H2O2 modified BM-biochar (CN600-O), and NH4OH modified BM-biochar (CN600-N) derived from corn stalk were applied to adsorb phenyl volatile organic compounds (VOCs). H2O2 and NH4OH modification of BM-biochar significantly improved its physicochemical characteristics and adsorption abilities. The specific surface area of CN600-O increased 2.05 and 1.23 times compared to CN600 and CN600-BM, respectively; while CN600-N increased 2.41 and 1.45 times, respectively. In addition, the ball milled biochars, especially CN600-O, showed higher acidity and polarity than CN600. The VOC adsorption amount onto biochars was 10.96-130.21 mg/g. CN600-O and CN600-N had high uptake of the VOCs and reached 100.07-111.79 mg/g and 110.49-130.21 mg/g, respectively. CN600-N showed the best performance with P-xylene adsorption up to 130.21 mg/g. VOC adsorption onto the CN600-O and CN600-N were mainly governed by surface adsorption and associated with morphology characteristics of the biochars as well as VOC properties such as boiling point and molecular size. Five cycles of adsorption-desorption experiments showed that CN600-O and CN600-N had good reusability with the reuse efficiencies of 88.01 %-92.21 % and 92.19 %-95.39 %, respectively. The results indicate that O- and N-doped ball-milled biochars are promising in adsorption for effective and sustainable VOC removal.

18.
Dev Comp Immunol ; 114: 103869, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32950537

RESUMO

Integrins are transmembrane glycoproteins that are broadly distributed in living organisms. As a heterodimer, they contain an α and a ß subunit, which are reported to be associated with various physiological and pathological processes. In the present study, a 2502 bp full-length cDNA sequence of Bmintegrin ß1 was obtained from the silkworm, Bombyx mori. Bmintegrin ß1 belongs to the ß subunit of the integrin family and contains several typical structures of integrins. Gene expression profile analysis demonstrated that Bmintegrin ß1 was ubiquitously expressed in all tested tissues and organs, with the maximum expression levels in fat body and hemocytes. The immunofluorescence results showed that Bmintegrin ß1 was located in the cell membrane and widely distributed in fat bodies and different types of hemocytes. Bmintegrin ß1 expression was remarkably increased after challenging with different kinds of bacteria and pathogen-associated molecular patterns (PAMPs). Further investigation revealed that Bmintegrin ß1 could participate in the agglutination of pathogenic bacteria possibly through direct binding with the relative bacteria and PAMPs. Altogether, this study provides a novel insight into the immune functional features of Bmintegrin ß1.

19.
Spectrochim Acta A Mol Biomol Spectrosc ; 245: 118906, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32932035

RESUMO

Three Mg3(PO4)2 polymorphs (Mg3(PO4)2-I, II, III) were synthesized at high-pressure and high-temperature conditions. The structures and vibrational properties of Mg3(PO4)2 polymorphs were studied by X-ray diffraction (XRD), Raman spectroscopy, and density functional theory (DFT) calculations. The obvious different PO stretching vibrational modes were experimentally observed for Mg3(PO4)2-I, II, III. The calculated vibrational frequencies were in good agreement with measurements. All the observed vibrational modes for Mg3(PO4)2-I, II, III were well assigned based on the calculations, which provided a support for investigating and comparing vibrational properties of three Mg3(PO4)2 polymorphs.

20.
J BUON ; 25(5): 2482-2489, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33277872

RESUMO

PURPOSE: The purpose of the present study was to identify differential gene expressions (DEGs) and key pathways in neuroblastoma with MEIS2 depletion through bioinformatics. METHODS: The microarray gene expression dataset GSE56003 was downloaded from the Gene Expression Omnibus (GEO) database. DEGs were identified using Gene Level RMA sketch and Transcriptome Analysis Console. Gene ontology (GO) function and KEGG pathway enrichment analysis of DEGs were performed using the DAVID online tool. Protein-protein interaction (PPI) networks were constructed by mapping the DEGs onto Cytoscape software. MCODE algorithm was used to select the module and Centiscape was used to screen the hub genes. The Kaplan-Meier survival curves was utilized to show the correlation of specific gene expressions and the survival situation of NB patients. Results:A total of 1352 DEGs were identified in neuroblastoma with MEIS2 depletion, which were mainly enriched during the cell cycle, DNA replication, and DNA repair. CDK2, RAD51, BRCA1, and MCM3 were selected as hub genes that have the potential as novel therapeutic targets for neuroblastoma. CONCLUSION: This study revealed the hub genes and pathway involved in neuroblastoma with MEIS2 knockdown, which offered new insights into the molecular networks underlying MEIS2 depletion in neuroblastoma. Additionally, this study provided a valuable resource of potential biomarkers and therapeutic targets.

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