Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 74
Filtrar
1.
Sci Rep ; 11(1): 11, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33420151

RESUMO

Haemophilus influenzae is a predominant pathogen for conjunctivitis, acute otitis media and acute bacterial paranasal sinusitis in children. We undertook this study to investigate the possible association among these diseases. Children younger than 18-year-old with a diagnosis of bacterial conjunctivitis plus acute otitis media and/or acute bacterial paranasal sinusitis during 2009-2018 were included. Sampling for bacterial cultures was obtained from the lower palpebral conjunctiva and/or ear discharge with cotton-tipped swabs. A total of 67 children were recruited and the age was 29.5 (± 22.4) months in average. Fifty-seven children had conjunctivitis-otitis media syndrome and eight of them had a concurrent diagnosis of acute paranasal sinusitis. Ten children had conjunctivitis and acute paranasal sinusitis simultaneously. Clusters in household were observed in 50.7% children. Most common isolates were Haemophilus influenzae (70%), Moraxella catarrhalis (18%), and Staphylococcus aureus (8%). Antibiotic resistance rate of H. influenzae was 80% for ampicillin, 18% for amoxicillin-clavulanate, and 11% for the second or third-generation cephalosporins. Apart from well-known conjunctivitis-otitis media syndrome, acute paranasal sinusitis may also be linked to conjunctivitis with a similar pathogenic process. Simultaneous presence of these infections may guide the choice of empiric antibiotics toward H. influenzae.

2.
Yi Chuan ; 42(5): 506-518, 2020 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-32431301

RESUMO

The root-associated bacterial microbiota is closely related to life activities of land plants, and its composition is affected by geographic locations and plant genotypes. However, the influence of plant genotypes on root microbiota in rice grown in northern China remains to be explained. In this study, we performed 16S rRNA gene amplicon sequencing to generate bacterial community profiles of two representative rice cultivars, Nipponbare and IR24. They are planted in Changping and Shangzhuang farms in Beijing and have reached the reproductive stage. We compared their root microbiota in details by Random Forest machine learning algorithm and network analysis. We found that the diversity of rice root microbiota was significantly affected by geographic locations and rice genotypes. Nipponbare and IR24 showed distinct taxonomic composition of the root microbiota and the interactions between different bacteria. Moreover, the root bacteria could be used as biomarkers to distinguish Nipponbare from IR24 across regions. Our study provides a theoretical basis for the in-depth understanding of rice root microbiota in Northern China and the improvement of rice breeding from the perspective of the interaction between root microorganisms and plants.


Assuntos
Bactérias/classificação , Microbiota , Oryza/microbiologia , Raízes de Plantas/microbiologia , China , RNA Ribossômico 16S
4.
Artigo em Inglês | MEDLINE | ID: mdl-32094073

RESUMO

BACKGROUND/PURPOSE: Early identification of pathogens causing bloodstream infection (BSI) is critical for prompt administration of appropriate antimicrobial therapy. METHODS: We used an in-house saponin-based extraction method to evaluate the performance of Bruker Biotyper MALDI-TOF MS system (MALDI Biotyper) for bacterial and fungal identification in 2013 positively-flagged VersaTREK blood culture bottles. RESULTS: A total of 180 monomicrobial and 23 polymicrobial positive blood cultures were investigated. Among monomicrobial positive blood cultures, the MALDI Biotyper recognized 90.6% and 81.7% of organisms directly from the flagged blood culture bottles to the genus and species levels, respectively. The MALDI Biotyper also correctly characterized one of the polymicrobial organisms to the species level in 20 (87%) bottles and to the genus level in 21 (91.3%) bottles. The overall identification rate using our protocol was 90.6% (184/203) and 82.3% (167/203) for genus and species levels, respectively. Identification accuracy was higher for Gram-positive than Gram-negative organisms and was the lowest for yeasts. Score values of identification were ≥1.500 for 200 (98.5%) bottles, ≥1.700 for 195 (96.1%) bottles and ≥2.000 for 182 (89.7%) bottles. Moreover, 83.5% and 92% of the isolates were identified precisely to species and genus level with the lower cutoff score of 1.500. Using our protocol also helped identifying BSI pathogens 18-24 h earlier compared to the sub-cultured colonies. CONCLUSION: Using Bruker MALDI Biotyper for identification of isolates directly from positive VersaTREK blood culture bottles, our in-house saponin-based protocol provided a more rapid turn-around time for correct identification of BSI pathogens than the conventional methods.

5.
Pediatr Neonatol ; 61(1): 9-15, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31706947

RESUMO

Enterovirus D68 was first identified in 1962 and caused a worldwide outbreak starting from the North America in 2014. Enterovirus D68 has been in continuous circulation among many countries recently, including Taiwan. Reports also reveal high seroprevalence, which indicates that the disease burden of enterovirus D68 may be underestimated via viral culture or polymerase chain reaction results. Although most infected cases have mild respiratory illness, severe complications including acute flaccid myelitis and acute respiratory distress syndrome have also been reported. In the position of an emerging pathogen, enterovirus D68 poses a threat to public health and may cause devastating diseases. Diverse severity of neurological sequelae remains inevitable among acute flaccid myelitis patients, but no curable treatment is available currently. According to the management suggestions of the American Centers of Disease Control, uses of corticosteroids and plasmapheresis are either preferred or avoided and intravenous immunoglobulin also has no clear indication in the treatment for acute flaccid myelitis. In this review article, we provide information about the epidemiology, clinical recognition and treatment strategy of enterovirus D68. Better understanding of this disease is the foothold for advanced investigation and monitoring in the future.


Assuntos
Enterovirus Humano D , Infecções por Enterovirus/epidemiologia , Viroses do Sistema Nervoso Central/terapia , Surtos de Doenças , Infecções por Enterovirus/complicações , Infecções por Enterovirus/terapia , Feminino , Humanos , Masculino , Mielite/terapia , Doenças Neuromusculares/terapia , Estudos Soroepidemiológicos , Taiwan/epidemiologia
6.
J Microbiol Immunol Infect ; 52(6): 858-864, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31164279

RESUMO

BACKGROUND/PURPOSE(S): Enterovirus D68(EV-D68) is an emerging disease that affects mostly children. There have been few relevant investigations to clarify transmission and seroprevalence within daycares and kindergartens. METHODS: This prospective cohort study investigated respiratory viral transmission among preschool children in a public kindergarten in Taipei City of Taiwan between September 2006 and June 2008. After children were enrolled, daily monitoring of illness and regular biweekly physical examinations were performed. We performed viral isolation to detect acute EV-D68 infection and neutralization tests to detect specific EV-D68 antibodies and to measure the seroprevalence and seroconversion rates. RESULTS: Among 190 kindergarten attendees aged between two to five years old, nine children had acute EV-D68 infection in September 2007. The clinical manifestations included pharyngitis, cough and other unspecified upper respiratory tract infection. None of the infected children had acute flaccid paralysis or severe respiratory illness. The phylogenetic tree of partial viral protein 3 and viral protein 1 was clustered in clade A1. The EV-D68 seropositive rate increased from 19% (25/130) at the beginning to 67% (83/124) at the end of the study. The seroconversion rate of 49 children with initial seronegative and paired sera was 73% (36/49). CONCLUSIONS: A high seroconversion rate (73%) for EV-D68 was found among kindergarten attendees, which indicates preschool-aged children are highly susceptible to EV-D68 infection and that the disease burden may be extremely underestimated. Once EV-D 68 circulates, preventive measures may be advocated, especially within kindergartens or daycares, to reduce transmission and subsequent development of severe cases.


Assuntos
Anticorpos Antivirais/sangue , Infecções por Enterovirus/epidemiologia , Infecções por Enterovirus/imunologia , Escolas Maternais , Soroconversão , Criança , Pré-Escolar , Enterovirus Humano D , Feminino , Humanos , Masculino , Testes de Neutralização , Filogenia , Estudos Prospectivos , Infecções Respiratórias/epidemiologia , Infecções Respiratórias/virologia , Estudos Soroepidemiológicos , Taiwan/epidemiologia
7.
Org Lett ; 21(4): 1007-1010, 2019 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-30693777

RESUMO

Phytochemical study of Hypericum ascyron led to the characterization of norascyronones A-C (1-3), metabolites derived from bicyclic polyprenylated acylphloroglucinols by losing eight carbons (C-2/3/4 of core and the isoprenyl at C-3). Compounds 1/2 with an unprecedented 6/6/5/6 ring system should be generated via [4 + 2] intramolecular cyclization of 3. Their structures were determined by spectroscopic and X-ray diffraction data. Compounds 1 and 2 showed cytotoxicities against the SK-BR-3 cell line (IC50 4.3 and 7.8 µM).

8.
J Biomed Res ; 32(4): 288-297, 2018 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-30008464

RESUMO

Preeclampsia is associated with over-activation of the innate immune system in the placenta, in which toll-like receptor 4 (TLR4) plays an essential part. With their potent anti-inflammatory effects, statins have been suggested as potential prevention or treatment of preeclampsia, although evidence remains inadequate. Herewith, we investigated whether pravastatin could ameliorate preeclampsia-like phenotypes in a previously established lipopolysaccharide (LPS)-induced rat preeclampsia model, through targeting the TLR4/NF-κB pathway. The results showed that pravastatin reduced the blood pressure [maximum decline on gestational day (GD) 12, (101.33±2.49) mmHg vs. (118.3±1.37) mmHg, P<0.05] and urine protein level [maximum decline on GD9, (3,726.23±1,572.86) µg vs. (1,991.03±609.37) µg, P<0.05], which were elevated following LPS administration. Pravastatin also significantly reduced the rate of fetal growth restriction in LPS-treated rats (34.10% vs. 8.99%, P<0.05). Further pathological analyses suggested a restoration of normal spiral artery remodeling in preeclampsia rats by pravastatin treatment. These effects of pravastatin were associated with decreased TLR4/NF-κB protein levels in the placenta and IL-6/MCP-1 levels in serum. Additionally, no obvious abnormalities in fetal liver, brain, and kidney were found after administration of pravastatin. These results provide supportive evidence for use of pravastatin in preventing preeclampsia.

9.
Nan Fang Yi Ke Da Xue Xue Bao ; 36(7): 984-9, 2016 Jun 20.
Artigo em Chinês | MEDLINE | ID: mdl-27435781

RESUMO

OBJECTIVE: To observe the effect of parathyroid hormone (PTH)(1-34) on the expression of matrix Gla protein (MGP) and Wnt/ß-catenin signaling pathway and elucidate the possible molecular mechanism of PTH (1-34) in the prevention and treatment of osteoporosis. METHODS: MG63 cells treated with PTH (1-34) at 10(-9), 10(-8), and 10(-7) mol/L, alone or in combination with Wnt/ß-catenin signaling pathway inhibitors DKK-1 (200 ng/ml) were examined for mRNA and protein expressions related with Wnt/ß-catenin signaling with real-time PCR and Western blotting. The cell differentiation after the treatment was assessed with alkaline phosphatase (ALP) staining and cell viability assay. RESULTS: PTH (1-34) significantly increased the expression of MGP in a dose-dependent manner in MG63 cells (P<0.05 or P<0.01). PTH treatment obviously enhanced ALP activity in the cells, and this effect was suppressed by DKK-1. Combined treatment with DKK-1 partially blocked PTH-induced enhancement of ALP activity (P<0.05). PTH promoted the expression of MGP and enhanced LRP5, ß-catenin, and Runx2 expressions in Wnt/ß-catenin signaling pathway at both protein and mRNA levels (P<0.05 or P<0.01). DKK-1 partially blocked the effect of PTH (1-34) on Wnt/ß-catenin signaling pathway (P<0.05) without affecting MGP expression. CONCLUSION: PTH (1-34) significantly increases the expressions of MGP and proteins in the Wnt/ß-catenin signaling pathway. Wnt/ß-catenin signaling pathway and MGP mediate the regulation of osteogenosis by PTH.


Assuntos
Proteínas de Ligação ao Cálcio/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Osteogênese , Hormônio Paratireóideo/farmacologia , Via de Sinalização Wnt , Fosfatase Alcalina/metabolismo , Diferenciação Celular , Linhagem Celular Tumoral , Sobrevivência Celular , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Osteoporose , Reação em Cadeia da Polimerase em Tempo Real
10.
Birth Defects Res B Dev Reprod Toxicol ; 101(3): 283-91, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24831781

RESUMO

This study was carried out to investigate the impact of tripterygium glycosides (TGs) on ovarian function of female rats in vitro and in vivo. In vitro studies showed that TG induced cells decrease at G1 phase and inhibited cell proliferation in rat granulosa cells. In vivo, female rats were intragastrically administered with TG at the dose of 60 mg/kg/day for consecutive 50 days. TG caused a prolonged estrous cycle, and a significant reduction in ovarian index, serum E2 level, and numbers of secondary and antral follicles (p < 0.05) in these rats. A significant reduction of viable embryos was demonstrated in TG-treated female rats after mating (p < 0.01). Further, we observed observed the reduced expression level of TGF-ß1 after TG treatment in vitro and in vivo. Moreover, the expression of Smad2 and AKT was also decreased after TG treatment. These results suggest that TG can impair ovarian function through Smads-mediated TGF-ß1 signal pathway.


Assuntos
Proliferação de Células/efeitos dos fármacos , Glicosídeos/toxicidade , Células da Granulosa/efeitos dos fármacos , Reprodução/efeitos dos fármacos , Tripterygium/química , Animais , Apoptose/efeitos dos fármacos , Ciclo Celular/efeitos dos fármacos , Células Cultivadas , Ciclina D2/genética , Ciclina D2/metabolismo , Diterpenos/toxicidade , Relação Dose-Resposta a Droga , Compostos de Epóxi/toxicidade , Feminino , Células da Granulosa/metabolismo , Fenantrenos/toxicidade , Ratos , Ratos Sprague-Dawley , Proteína Smad2/genética , Proteína Smad2/metabolismo , Fator de Crescimento Transformador beta1/genética , Fator de Crescimento Transformador beta1/metabolismo
11.
Tumour Biol ; 35(4): 3167-70, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24293392

RESUMO

Case-control studies on the association between mouse double minute 2 homolog (MDM2) rs2279744 polymorphism and endometrial cancer have provided either controversial or inconclusive results. To clarify the effect of MDM2 rs2279744 polymorphism on the risk of endometrial cancer, a meta-analysis of all case-control observational studies was performed. Pooled odds ratios (ORs) for various polymorphisms were estimated using random and fixed effect models. Q-statistic was used to evaluate the homogeneity, and Egger and Begg tests were used to assess publication bias. Overall, the MDM2 rs2279744 polymorphism was associated with a risk of endometrial cancer (OR = 0.76; 95% CI = 0.64-0.90 for allele contrast, p = 0.002, P(het) = 0.003). The contrast of homozygotes and the recessive and dominant models produced the same pattern of results as the allele contrast. In the analysis stratified by ethnicity, significant associations were found in the Caucasian population in all of the genetic models. Our pooled data suggest evidence for a major role of MDM2 rs2279744 polymorphism in the carcinogenesis of endometrial cancer, especially among Caucasian populations.


Assuntos
Neoplasias do Endométrio/genética , Polimorfismo Genético , Proteínas Proto-Oncogênicas c-mdm2/genética , Neoplasias do Endométrio/etnologia , Neoplasias do Endométrio/etiologia , Grupo com Ancestrais do Continente Europeu/genética , Feminino , Humanos , Risco
12.
Cancer Lett ; 345(1): 39-47, 2014 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-24333727

RESUMO

MicroRNA miR-302 has been found to induce some tumor cell lines to "transdifferentiate" into miRNA-induced pluripotent stem cells (mirPS), thereby inhibiting tumor cell proliferation and reducing tumorigenicity. This study firstly found that miR-302 inhibited the proliferation and migration of endometrial cell line, Ishikawa and HEC-1-B, and arrested cell cycle at the G2/M phase. In addition, miR-302 inhibited tumorigenicity in immunodeficient mice transplanted with Ishikawa cells. Microarray and Western blotting results showed that miR-302 significantly inhibited CDK1 and Cyclin D1 gene expression in Ishikawa cells. MiR-302 directly targeted Cyclin D1, but indirectly regulated CDK1 gene expression.


Assuntos
Ciclina D1/antagonistas & inibidores , Inibidor de Quinase Dependente de Ciclina p57/antagonistas & inibidores , Neoplasias do Endométrio/terapia , Terapia Genética/métodos , MicroRNAs/administração & dosagem , Animais , Processos de Crescimento Celular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Ciclina D1/genética , Ciclina D1/metabolismo , Inibidor de Quinase Dependente de Ciclina p57/genética , Inibidor de Quinase Dependente de Ciclina p57/metabolismo , Neoplasias do Endométrio/genética , Neoplasias do Endométrio/metabolismo , Neoplasias do Endométrio/patologia , Feminino , Perfilação da Expressão Gênica , Humanos , Camundongos , Camundongos Nus , MicroRNAs/genética , Transdução de Sinais , Transfecção/métodos , Ensaios Antitumorais Modelo de Xenoenxerto
13.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 30(5): 598-600, 2013 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-24078579

RESUMO

OBJECTIVE: To analyze TRAPPC2 gene mutation in a family with X-linked spondyloepiphyseal dysplasia tarda and to provide genetic counseling and prenatal diagnosis. METHODS: All of 4 exons of the TRAPPC2 gene and their flanking sequences in the proband and her father were analyzed with polymerase chain reaction and direct DNA sequencing. Genomic DNA of the probands' fetus was extracted from amniotic fluid sampled at 18th gestational week. Gender of the fetus was determined by the presence of SRY gene. The sequence of fetal TRAPPC2 gene was also analyzed. RESULTS: A c.209G>A mutation was identified in exon 4 of the TRAPPC2 gene in the proband and her father. The fetus of was determined to be a male and also have carried the c.209G>A mutation. CONCLUSION: A c.209G>A mutation of TRAPPC2 exon 4 probably underlies the clinical manifestations in this family. The proband is a carrier, and her fetus is a male carrying the same mutation. Prenatal diagnosis is an effective method for the prevention of the disease.


Assuntos
Doenças Genéticas Ligadas ao Cromossomo X/genética , Osteocondrodisplasias/genética , Sequência de Bases , Feminino , Aconselhamento Genético , Doenças Genéticas Ligadas ao Cromossomo X/diagnóstico , Doenças Genéticas Ligadas ao Cromossomo X/embriologia , Humanos , Dados de Sequência Molecular , Mutação Puntual , Gravidez , Diagnóstico Pré-Natal
14.
J Clin Endocrinol Metab ; 98(11): 4417-28, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24037888

RESUMO

BACKGROUND: Homeobox A10 (HOXA10), a key transcription factor, plays a critical role in endometrial receptivity by regulating the expression of downstream target genes, such as ß3-integrin (ITGB3), but little is understood about the mechanisms of the posttranslational modification of HOXA10 during embryo implantation. OBJECTIVE: The aim of this study was to assess the effect of HOXA10 acetylation by p300/CREB-binding protein-associated factor (PCAF) in the embryo implantation process. METHODS: The association of HOXA10 with PCAF was detected by coimmunoprecipitation, Western blotting, and confocal immunofluorescent assays. A luciferase reporter assay, Western blotting, quantitative real-time PCR, and chromatin immunoprecipitation techniques were used to determine the effect of PCAF on HOXA10 protein stability and the HOXA10-mediated regulation of ITGB3 expression. HOXA10-PCAF association on embryo implantation was evaluated using a BeWo spheroid attachment assay. PCAF expression in the eutopic endometrium of women with endometriosis and fertile controls was measured by Western blotting technique. RESULTS: PCAF was identified as an HOXA10-interacting protein and inhibited HOXA10-mediated ITGB3 transcription via acetylating HOXA10 at K338 and K339. Overexpressing or knocking down PCAF in Ishikawa cells showed that PCAF not only down-regulated HOXA10-mediated ITGB3 protein expression but also diminished HOXA10-mediated embryo adhesiveness by acetylating HOXA10 (P < .05). Furthermore, we found aberrantly high PCAF expression in the eutopic endometrium of women with a diagnosis of endometriosis compared with the fertile controls (P < .05). CONCLUSIONS: These observations demonstrate that 1) HOXA10 associates with and is acetylated by PCAF at lysines K338 and K339 in Ishikawa cells and 2) HOXA10-PCAF association impairs embryo implantation by inhibiting ITGB3 protein expression in endometrial epithelial cells.


Assuntos
Implantação do Embrião/fisiologia , Endométrio/fisiologia , Proteínas de Homeodomínio/genética , Integrina beta3/genética , Fatores de Transcrição de p300-CBP/genética , Acetilação , Adulto , Regulação para Baixo/fisiologia , Neoplasias do Endométrio , Endométrio/citologia , Células Epiteliais/citologia , Células Epiteliais/fisiologia , Estrogênios/fisiologia , Feminino , Regulação da Expressão Gênica/fisiologia , Células HEK293 , Proteínas Homeobox A10 , Proteínas de Homeodomínio/metabolismo , Humanos , Integrina beta3/metabolismo , Gravidez , Progesterona/fisiologia , RNA Interferente Pequeno/genética , Transdução de Sinais/fisiologia , Adulto Jovem , Fatores de Transcrição de p300-CBP/metabolismo
17.
Mol Reprod Dev ; 80(9): 734-43, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23836374

RESUMO

It has been previously reported that follicle-stimulating hormone (FSH) regulates the expression of inhibin-alpha in human granulosa cells, but the precise molecular pathway remains unknown. In the present study, we investigated the role of the orphan nuclear receptor, NUR77, in both the transcriptional regulation of the inhibin α-subunit gene and the secretion of inhibins. Our results showed that in a human granulosa cell tumor-derived cell line (KGN) and in human granulosa-lutein cells (hGL), FSH induced the expression of NUR77 and inhibin-alpha, although inhibin-alpha expression did not increased following FSH treatment if NUR77 was knocked down. Furthermore, simply overexpressing or reducing NUR77 levels affected inhibin-alpha expression, while NUR77 overexpression improved the secretion of inhibin A and B from human granulosa cells. In addition, chromatin immunoprecipitation-PCR, avidin-biotin-conjugated DNA precipitation, and luciferase reporter assays confirmed that NUR77 directly regulated the transcription of the inhibin-alpha gene through the specific NGFI-B response element located within its promoter. In the ovarian granulosa cells of the Nur77 knockout mice, the mRNA levels of inhibin-alpha were decreased relative to wild-type mice. These data indicate a role of NUR77 in the regulation of inhibin-alpha in ovarian granulosa cells.


Assuntos
Hormônio Foliculoestimulante/metabolismo , Regulação da Expressão Gênica/fisiologia , Células da Granulosa/metabolismo , Inibinas/metabolismo , Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares/metabolismo , Análise de Variância , Animais , Avidina , Biotina , Western Blotting , Imunoprecipitação da Cromatina , Primers do DNA/genética , Feminino , Ferritinas , Técnicas de Silenciamento de Genes , Humanos , Luciferases , Camundongos , Camundongos Knockout , Membro 1 do Grupo A da Subfamília 4 de Receptores Nucleares/genética , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase em Tempo Real
18.
Reprod Biomed Online ; 27(2): 131-9, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23764202

RESUMO

This study evaluated whether the withdrawal of a gonadotrophin-releasing hormone (GnRH) agonist before triggering ovulation reduces the incidence of ovarian hyperstimulation syndrome (OHSS) in high-risk infertility patients who were treated with gonadotrophins. GnRH agonist was withdrawn for 2 or 3 days when dominant follicles were ≥14 mm in diameter, according to the GnRH agonist long protocol. Non-withdrawal of GnRH agonist was used as control. The serum concentration of oestradiol on the ovulation trigger day was significantly decreased in the GnRH agonist withdrawal group compared with the control group (5750.78 ± 2344.77 pg/ml versus 8076.43 ± 1981.67 pg/ml); however, the number of retrieved oocytes and the fertilization rate were similar between the groups. In addition, the concentrations of vascular endothelial growth factor in plasma on day of human chorionic gonadotrophin administration and follicular fluid on the oocyte retrieval day were decreased following GnRH agonist withdrawal. In fresh embryo transfer cycles, rates of clinical pregnancy, implantation and OHSS were not different between the groups. When GnRH agonist withdrawal was followed by total embryos cryopreserved, the rate of OHSS was decreased compared with the control group (0% versus 8.70%). Clinical pregnancy rates in cryopreserved embryo transfer cycles were comparable between the two groups.


Assuntos
Estradiol/sangue , Fármacos para a Fertilidade Feminina/administração & dosagem , Hormônio Liberador de Gonadotropina/agonistas , Infertilidade Feminina/terapia , Síndrome de Hiperestimulação Ovariana/prevenção & controle , Indução da Ovulação/efeitos adversos , Fator A de Crescimento do Endotélio Vascular/sangue , Adulto , China/epidemiologia , Criopreservação , Método Duplo-Cego , Esquema de Medicação , Resistência a Medicamentos , Transferência Embrionária , Embrião de Mamíferos , Feminino , Fármacos para a Fertilidade Feminina/efeitos adversos , Fertilização In Vitro , Humanos , Incidência , Infertilidade Feminina/sangue , Síndrome de Hiperestimulação Ovariana/epidemiologia , Síndrome de Hiperestimulação Ovariana/etiologia , Gravidez , Taxa de Gravidez
19.
BMC Infect Dis ; 13: 109, 2013 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-23446269

RESUMO

BACKGROUND: The prevalence, genotypes, and vertical transmission characteristics of human papillomavirus (HPV) among pregnant women from Nanjing, China was investigated. METHODS: Cervical cells were collected from healthy pregnant women (n = 3139; stage of gestation, 24.6 ± 2.1 weeks) for cytological evaluation and determination of HPV infection status. Exfoliated oral and genital cells were collected from neonates (<1-day-old, n = 233) whose mothers were positive for HPV DNA. We used HPV Gene Chip technology with 23 HPV genotype probes to conduct our analysis. RESULTS: Overall prevalence of HPV DNA among pregnant women was 13.4% (422/3139). The most frequently detected HPV genotypes were HPV-16 (29.6%, 125/422), -18 (14.7%, 62/422), and -58 (14.2%, 60/422). The rate of concordance for HPV DNA in maternal-neonatal pairs was 23.6% (55/233), with HPV type-specific concordance occurring in 26 cases. A higher prevalence of HPV DNA was apparent in female neonates compared with males (17.7 vs. 11.6%). CONCLUSIONS: The prevalence of cervical HPV DNA in pregnant women from Nanjing was low, with vertical transmission rates slightly higher. From our findings, we concluded that there was efficient vertical transmission of three HPV genotypes, with HPV-16 the most prevalent type in pregnant women and newborn babies.


Assuntos
Alphapapillomavirus/isolamento & purificação , Transmissão Vertical de Doença Infecciosa , Infecções por Papillomavirus/transmissão , Infecções por Papillomavirus/virologia , Complicações Infecciosas na Gravidez/virologia , Adulto , Alphapapillomavirus/classificação , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Recém-Nascido , Masculino , Razão de Chances , Infecções por Papillomavirus/epidemiologia , Infecções por Papillomavirus/patologia , Gravidez , Complicações Infecciosas na Gravidez/epidemiologia , Complicações Infecciosas na Gravidez/patologia , Prevalência , Fatores de Risco , Adulto Jovem
20.
Diabetes Care ; 36(7): 2038-40, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23536582

RESUMO

OBJECTIVE: To evaluate the usefulness of a fasting plasma glucose (FPG) at 24-28 weeks' gestation to screen for gestational diabetes mellitus (GDM). RESEARCH DESIGN AND METHODS: The medical records and results of a 75-g 2-h oral glucose tolerance test (OGTT) of 24,854 pregnant women without known pre-GDM attending prenatal clinics in 15 hospitals in China were examined. RESULTS: FPG cutoff value of 5.1 mmol/L identified 3,149 (12.1%) pregnant women with GDM. FPG cutoff value of 4.4 mmol/L ruled out GDM in 15,369 (38.2%) women. With use of this cutoff point, 12.2% of patients with mild GDM will be missed. The positive predictive value is 0.322, and the negative predictive value is 0.928. CONCLUSIONS: FPG at 24-28 weeks' gestation could be used as a screening test to identify GDM patients in low-resource regions. Women with an FPG between ≥4.4 and ≤5.0 mmol/L would require a 75-g OGTT to diagnose GDM. This would help to avoid approximately one-half (50.3%) of the formal 75-g OGTTs in China.


Assuntos
Diabetes Gestacional/sangue , Jejum/sangue , Glicemia/metabolismo , China/epidemiologia , Diabetes Gestacional/epidemiologia , Feminino , Teste de Tolerância a Glucose , Humanos , Gravidez
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...