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1.
Front Cell Infect Microbiol ; 12: 1029592, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36452293

RESUMO

Background: Radiation-induced skin injury (RISI) is still the most common and severe side effect of radiotherapy. The role of the skin's microbial barrier in the pathogenesis and progression of RISI needs to be fully investigated. Methods: This study aimed to explore the alterations in and functions of the skin microbiota in RISI. We applied the unculturable approach to characterize the cutaneous microbiomes of a radiation-induced animal model by sequencing the V1-V3 regions of the 16S ribosomal RNA (rRNA) gene. Combined with the downloaded clinical data of patients, a comprehensive analysis was performed to identify potential radioprotective species and metabolic pathways. Results: There were no significant differences in the alpha diversity indices (Sobs, Shannon, Simpson, Ace, and Chao) between the acute radiation injury and control groups. Phylum-level analysis of the RISI microbiomes exhibited significant predominance of Firmicutes (mean abundance = 67%, corrected p = 0.0035). The high abundance of Firmicutes was significantly associated with rapid healing of RISI (average relative abundance = 52%; Kruskal-Wallis: p = 5.7E-4). Among its members, Streptococcus, Staphylococcus, Acetivibrio ethanolgignens group, Peptostreptococcus, Anaerofilum, and UCG-002 [linear discriminant analysis (LDA) > 3, p < 0.05] were identified as the core genera of Firmicutes. In addition, Lachnosiraceae and Lactobacillus occupied an important position in the interaction network (r > 0.6, p < 0.05). The differential metabolic pathways of RISI were mainly associated with carbohydrate metabolism (butanoate and propanoate metabolism), amino acid metabolism (tryptophan and histidine metabolism), energy metabolism, and lipid metabolism (fatty acid degradation and biosynthesis). Conclusion: This study provides new insights into the potential mechanism and skin microbial changes in the progression of RISI. The overwhelming predominance of members of Firmicutes, including Streptococcaceae, Staphylococcaceae, Lachnospiraceae, and Lactobacillus, is potentially related to rapid healing of RISI. The microbiota-metabolite axis plays a critical role in RISI and provides promising therapeutic targets for the treatment of adverse side effects.


Assuntos
Microbiota , Lesões por Radiação , Animais , Pele , Radiação Ionizante , Cicatrização , Firmicutes , Lactobacillus
2.
Water Sci Technol ; 86(9): 2454-2464, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36378192

RESUMO

Sumps are commonly used in urban stormwater systems, which can be considered as a simple pretreatment device for stormwater quality control. However, they may function as pollution sources due to sediment washout under high flow conditions. An experimental study was conducted to investigate the scour process of predeposited sediments from a sump and its influencing parameters. Under conditions with large inflows or high sediment deposit, the sediment particles could be resuspended, entrained and flushed out. The washout mass decreased exponentially with time if the sediment bed surface depth was larger than a threshold value; otherwise, the amount of washout would be much smaller. The same scour pattern was observed for all the testing cases, of which the largest scour depth always occurred below the outlet. The deposit below the inlet might increase under conditions with high flow rates and low levels of sediment bed. Dimension analysis was performed and principal non-dimensional parameters were found, including the Péclet number, the pipe Froude number, and the dimensionless particle diameter, which can be used to determine whether the washout would occur and its intensity in a stormwater sump under given conditions.


Assuntos
Baías , Sedimentos Geológicos , Sedimentos Geológicos/análise , Chuva
3.
Clin Chem Lab Med ; 2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36351699

RESUMO

OBJECTIVES: This study aimed to establish time-resolved fluorescence immunoassays to quantitatively detect the autoantibodies targeting different epitopes of M-type phospholipase A2 receptor (PLA2R) and evaluate its clinical application in primary membranous nephropathy (PMN). METHODS: PLA2R and its reactive epitope-specific IgG/IgG4 time-resolved fluorescence immunoassays (TRFIAs) were established using europium-labeled anti-human IgG/IgG4 antibodies, recombinant proteins, and patient serum. The levels of IgG/IgG4 targeting PLA2R and its epitopes in PMN patient serum were detected, and the relationship between epitope spreading of PLA2R and the severity of patients with PMN was evaluated. RESULTS: The TRFIAs established in this study could quantitatively detect PLA2R and its epitope-specific IgG and IgG4. Sera from 59 patients with PMN were subjected to detection using anti-PLA2R IgG and anti-PLA2R IgG4. Among them, 46 and 54 patients were found positive for PLA2R antibodies, respectively. Moreover, the levels of PLA2R antibodies were strongly correlated with the severity of patients with PMN. Patients who were detected to have two or more epitopes had more serious renal injury. CONCLUSIONS: PLA2R domain-specific IgG/IgG4 TRFIAs were established in this study, and detection with anti-PLA2R IgG4 could more sensitively screen the reactivity of patients to the PLA2R domain. Moreover, detection epitope spreading of PLA2R was confirmed which is related to the severity of patients with PMN.

4.
Front Endocrinol (Lausanne) ; 13: 990133, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36339425

RESUMO

Background and objectives: Diabetes mellitus is a serious metabolic disease that causes a serious economic burden worldwide. Gut flora is a major component of diabetes research, and the aim of this study was to understand the trends and major components of research related to diabetes and gut flora in the last 11 years. Methods: We searched the Web of Science Core Collection database for articles on diabetes and gut flora related research from 2011-2021 on July 2, 2022. The literature data were analyzed for country, institution, author, steward, journal, and highly cited literature using Citespace.5.8.R3 and Vosviewer1.6.17. Results: Finally 4834 articles that met the requirements were included. The overall trend of articles published in the last 11 years is increasing, and the trend of articles published after 2019 is increasing significantly. In total, 109 countries, 4820 institutions, and 23365 authors were involved in the field of research. The highest number of publications was 1262 articles from the United States, the institution with the most publications was the University of Copenhagen with 134 articles, and the author with the most publications was PATRICE D CANI with 52 articles. Conclusion: The number of studies related to diabetes and intestinal flora is increasing and more and more researchers are involved in this field. Intestinal flora provides a key research direction for the treatment of diabetes. In the future, gut flora will remain the focus of the diabetes field.


Assuntos
Diabetes Mellitus , Microbioma Gastrointestinal , Humanos , Bibliometria , Publicações , Diabetes Mellitus/epidemiologia , Bases de Dados Factuais
5.
Huan Jing Ke Xue ; 43(11): 5169-5179, 2022 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-36437089

RESUMO

The Yangtze River Economic Belt is one of the major strategic development regions in China. It is of great significance to clarify the characteristics and sources of heavy metal pollution in farmland soil of the Yangtze River Economic Belt for the prevention and control of heavy metal pollution and to ensure safe agricultural production. After collecting extensive literature data, we analyzed the pollution characteristics, environmental risk, and potential sources of heavy metals (Cd, Cr, Hg, As, Pb, Cu, Zn, and Ni) in farmland soil of the Yangtze River Economic Belt through the integrated use of spatial and geo-accumulation index analyses. The results showed that:① the proportion of soil samples exceeding the risk screening values for soil contamination of agricultural land of Cd, Cu, Pb, Hg, Zn, and As were 39.8%, 18.5%, 8.3%, 6.9%, 6.9%, and 6.4%, respectively. Compared with the risk standard, soil Cd had the highest rate of exceeding the standard. ② The contents of Cr, Cu, Zn, and Ni in the upper reaches were higher than those in the middle and lower reaches, and the contents of Cd, As, and Pb in the middle reaches were higher than those in the upper and lower reaches. ③ The results of the geo-accumulation index analysis showed that the contamination degree of the eight heavy metals decreased in the order of Cd(0.42)>Hg(-0.28)>Pb(-0.32)>Zn(-0.39)>Cu(-0.42)>Cr(-0.7)>As(-0.81)>Ni(-0.73), where the accumulation risk of soil Cd and Hg was relatively higher. ④ Higher environmental background and mining activities were the main factors affecting the accumulation of heavy metals in soils in the upper and middle reaches. By contrast, rapid urbanization, industrial production, and intensive agricultural activities were the main factors affecting the heavy metal accumulation in soils in the middle and lower reaches. In view of the current status and control needs of heavy metal pollution in the Yangtze River Economic Belt, it is recommended to strengthen source prevention and control and to also carry out hierarchical control and regional classification management of heavy metal pollution in farmland soils according to the degree of heavy metal pollution, geological background, and quality of agricultural products. The ultimate objective of this research was to archive the farmland soil environmental quality safety and agricultural green sustainable production in the Yangtze River Economic Belt.


Assuntos
Mercúrio , Metais Pesados , Solo , Fazendas , Rios , Cádmio , Chumbo , Bibliometria
6.
RSC Adv ; 12(47): 30253-30261, 2022 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-36337951

RESUMO

Soil contamination by heavy metals such as Cd can pose a risk to the environment and human health. However, Cd is difficult to immobilize at low concentration levels in soil. Individually, Bacillus subtilis and biochar have been shown to be inefficient at immobilizing Cd in soil. In this study, corncob biochar was generated at different pyrolysis temperatures (300 °C-550 °C), and the Cd immobilization efficiency and performance of corncob biochar loaded with B. subtilis (CB@B) and corncob biochar alone (CB) were evaluated in solutions and in soil. The characterization (SEM and FTIR) of CB generated at different pyrolysis temperatures and CB generated at different pyrolysis temperatures in CB@B (300 °C-550 °C) indicated that a superior pore structure and abundant O-functional groups were obtained at a pyrolysis temperature of 400 °C for both CB@B and CB. The X-ray diffraction and X-ray photoelectron spectroscopy results indicate that the formation of Cd compounds was associated with the positive combined biosorption effect of the bacteria and biochar, electronic adsorption, activity of the O-functional groups (C[double bond, length as m-dash]O, COOH, OH, and Si-O-Si), and complexation between extracellular substances and Cd2+. Adsorption experiments were conducted in a solution to assess the effects of various operating parameters such as the time, pH, and adsorbent dose. The 400 °C-CB@B and 400 °C-CB samples achieved the largest reductions in the Cd concentration at 81.21% and 5.70%, respectively. Then, CaCl2 extraction experiments were conducted in soil, and using 0.25%-CB@B, a 55.21% decrease was realized in the Cd concentration after 56 days and a 16.71% increase was realized in soil pH to 8.38. No significant difference was observed in the CB-treated groups, among which 1.0%-CB achieved the largest reduction of 26.08% after 56 days and a 3.20% increase in the soil pH to 7.41. The Tessier sequential extraction method obtained similar trends. Overall, 400 °C-CB@B demonstrated outstanding immobilization efficiency and durability, indicating that it provided a safe and nutrient-rich habitat for B. subtilis to realize a synergistic effect for Cd immobilization.

7.
Front Genet ; 13: 1045244, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36338963

RESUMO

Background: Infantile hemangiomas (IH) and venous malformations (VM) are the most common types of vascular abnormalities that seriously affect the health of children. Although there is evidence that these two diseases share some common genetic changes, the underlying mechanisms need to be further studied. Methods: The microarray datasets of IH (GSE127487) and VM (GSE7190) were downloaded from GEO database. Extensive bioinformatics methods were used to investigate the common differentially expressed genes (DEGs) of IH and VM, and to estimate their Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Trough the constructing of protein-protein interaction (PPI) network, gene models and hub genes were obtained by using Cytoscape and STRING. Finally, we analyzed the co-expression and the TF-mRNA-microRNA regulatory network of hub genes. Results: A total of 144 common DEGs were identified between IH and VM. Functional analysis indicated their important role in cell growth, regulation of vasculature development and regulation of angiogenesis. Five hub genes (CTNNB1, IL6, CD34, IGF2, MAPK11) and two microRNA (has-miR-141-3p, has-miR-150-5p) were significantly differentially expressed between IH and normal control (p < 0.05). Conclusion: In conclusion, our study investigated the common DEGs and molecular mechanism in IH and VM. Identified hub genes and signaling pathways can regulate both diseases simultaneously. This study provides insight into the crosstalk of IH and VM and obtains several biomarkers relevant to the diagnosis and pathophysiology of vascular abnormalities.

8.
Brain Sci ; 12(11)2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36421880

RESUMO

For patients with disorders of consciousness, such as unresponsive wakefulness syndrome (UWS) patients and minimally conscious state (MCS) patients, their long treatment cycle and high cost commonly put a heavy burden on the patient's family and society. Therefore, it is vital to accurately diagnose and predict consciousness recovery for such patients. In this paper, we explored the role of the P300 signal based on an audiovisual BCI in the classification and prognosis prediction of patients with disorders of consciousness. This experiment included 18 patients: 10 UWS patients and 8 MCS- patients. At the three-month follow-up, we defined patients with an improved prognosis (from UWS to MCS-, from UWS to MCS+, or from MCS- to MCS+) as "improved patients" and those who stayed in UWS/MCS as "not improved patients". First, we compared and analyzed different types of patients, and the results showed that the P300 detection accuracy rate of "improved" patients was significantly higher than that of "not improved" patients. Furthermore, the P300 detection accuracy of traumatic brain injury (TBI) patients was significantly higher than that of non-traumatic brain injury (NTBI, including acquired brain injury and cerebrovascular disease) patients. We also found that there was a positive linear correlation between P300 detection accuracy and CRS-R score, and patients with higher P300 detection accuracy were likely to achieve higher CRS-R scores. In addition, we found that the patients with higher P300 detection accuracies tend to have better prognosis in this audiovisual BCI. These findings indicate that the detection accuracy of P300 is significantly correlated with the level of consciousness, etiology, and prognosis of patients. P300 can be used to represent the preservation level of consciousness in clinical neurophysiology and predict the possibility of recovery in patients with disorders of consciousness.

9.
J Healthc Eng ; 2022: 3524022, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36247089

RESUMO

Background: Cutaneous squamous cell carcinoma (cSCC) is the leading cause of death in patients with nonmelanoma skin cancers (NMSC). However, the unclear pathogenesis of cSCC limits the application of molecular targeted therapy. Methods: Three microarray datasets (GSE2503, GSE45164, and GSE66359) were downloaded from the Gene Expression Omnibus (GEO). After identifying the differentially expressed genes (DEGs) in tumor and nontumor tissues, five kinds of analyses, namely, functional annotation, protein-protein interaction (PPI) network, hub gene selection, TF-miRNA-mRNA regulatory network analysis, and ferroptosis mechanism, were performed. Results: A total of 146 DEGs were identified with significant differences, including 113 upregulated genes and 33 downregulated genes. The enriched functions and pathways of the DEGs included microtubule-based movement, ATP binding, cell cycle, P53 signaling pathway, oocyte meiosis, and PLK1 signaling events. Nine hub genes were identified (CDK1, AURKA, RRM2, CENPE, CCNB1, KIAA0101, ZWINT, TOP2A, and ASPM). Finally, RRM2, AURKA, and SAT1 were identified as significant ferroptosis-related genes in cSCC. The differential expression of these genes has been verified in two other independent datasets. Conclusions: By integrated bioinformatic analysis, the hub genes identified in this study elucidated the molecular mechanism of the pathogenesis and progression of cSCC and are expected to become future biomarkers or therapeutic targets.


Assuntos
Carcinoma de Células Escamosas , Ferroptose , MicroRNAs , Neoplasias Cutâneas , Trifosfato de Adenosina , Aurora Quinase A/genética , Aurora Quinase A/metabolismo , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/genética , Ferroptose/genética , Perfilação da Expressão Gênica , Ontologia Genética , Redes Reguladoras de Genes , Humanos , MicroRNAs/genética , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , RNA Mensageiro , Neoplasias Cutâneas/genética , Transcriptoma , Proteína Supressora de Tumor p53/genética , Proteína Supressora de Tumor p53/metabolismo
10.
Phys Rev Lett ; 129(13): 133001, 2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36206415

RESUMO

We analytically identify a new class of quantum scars protected by spatiotemporal translation symmetries, dubbed Floquet-Bloch scars. They are distinguished from previous (quasi-)static scars by a rigid spectral pairing only possible in Floquet systems, where strong interaction and drivings equalize the quasienergy corrections to all scars and maintain their spectral spacings against generic bilinear perturbations. Scars then enforce the spatial localization and rigid discrete time crystal (DTC) oscillations as verified numerically in a trimerized kagome lattice model relevant to recent cold atom experiments. Our analytical solutions offer a potential scheme to understand the mechanisms for more generic translation-invariant DTCs.

11.
Front Genet ; 13: 1038222, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36246590

RESUMO

Background: Severe burns and blunt trauma can lead to multiple organ dysfunction syndrome, the leading cause of death in intensive care units. In addition to infection, the degree of immune inflammatory response also affects prognosis. However, the characteristics and clinical relevance of the common mechanisms of these major diseases are still underexplored. Methods: In the present study, we performed microarray data analysis to identify immune-related differentially expressed genes (DEGs) involved in both disease progression in burns and blunt trauma. Six analyses were subsequently performed, including gene enrichment analysis, protein-protein interaction (PPI) network construction, immune cell infiltration analysis, core gene identification, co-expression network analysis, and clinical correlation analysis. Results: A total of 117 common immune-related DEGs was selected for subsequent analyses. Functional analysis emphasizes the important role of Th17 cell differentiation, Th1 and Th2 cell differentiation, Cytokine-cytokine receptor interaction and T cell receptor signaling pathway in these two diseases. Finally, eight core DEGs were identified using cytoHubba, including CD8A, IL10, CCL5, CD28, LCK, CCL4, IL2RB, and STAT1. The correlation analysis showed that the identified core DEGs were more or less significantly associated with simultaneous dysregulation of immune cells in blunt trauma and sepsis patients. Of these, the downregulation of CD8A and CD28 had a worse prognosis. Conclusion: Our analysis lays the groundwork for future studies to elucidate molecular mechanisms shared in burns and blunt trauma. The functional roles of identified core immune-related DEGs and dysregulated immune cell subsets warrant further in-depth study.

12.
PeerJ ; 10: e14193, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36248712

RESUMO

Background: The Kidney Disease Improving Global Outcomes (KDIGO) 2021 guidelines recommend Rituximab (RTX) as the first-line therapy and phospholipase A2 receptor (PLA2R) antibody as a biomarker for remission and prognosis in patients with idiopathic membranous nephropathy (IMN). Methods: This study was a retrospective analysis of 70 patients with IMN treated with either rituximab (RTX) or cyclophosphamide (CTX) and steroid. Quantitative detection of PLA2R-IgG and PLA2R-IgG4 antibodies at sixth month after treatment, determined using time-resolved fluoroimmunoassay (TRFIA), were used for treatment effectiveness analysis and prognostic evaluation in patients with IMN. Results: After 12 months of therapy, the remission rate of proteinuria, including complete remission (CR) and partial remission (PR) in the RTX group and the CTX group, were 74% versus 67.5% (P = 0.114), respectively. Both PLA2R-IgG and PLA2R-IgG4 levels were decreased in patients with remission of proteinuria after 6 months of therapy. Receiver operating characteristic curve (ROC) curve analysis exhibited that the AUC of PLA2R-IgG4 and the PLA2R-IgG as laboratory criteria for proteinuria remission were 0.970 versus 0.886 (P = 0.0516), respectively, after 6 months of treatment. The cut-off value of PLA2R-IgG4 was 7.67 RU/mL and the sensitivity and specificity of remission rate at 6th month were 90.9% and 100%, respectively. Furthermore, the AUC of the PLA2R-IgG4 and PLA2R-IgG to predict the outcome after 12 months of treatment were 0.922 versus 0.897 (P = 0.3270), respectively. With the cut-off value of PLA2R-IgG4 being 22.985 RU/mL, the sensitivity and specificity of remission rate at 12th month were 100% and 87.1%, respectively. Logistic regression analysis revealed that the PLA2R-IgG4 level (P = 0.023), the rate of decrease of PLA2R-IgG4 level (P = 0.034), and eGFR level (P = 0.012) were significantly associated with remission. Conclusions: We found that the patients in the RTX group and CTX group achieved effective remission of proteinuria after 12 months of treatment. PLA2R-IgG4 may be a more effective biomarker for treatment effectiveness analysis and prognostic assessment, compared with anti-PLA2R-IgG for PLA2R associated IMN.

13.
Mol Ther Nucleic Acids ; 30: 143-161, 2022 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-36250210

RESUMO

Preeclampsia (PE) is a pregnancy-specific disorder attributed to deficient extravillous trophoblast (EVT) invasion into the uterus, but the mechanism of EVT invasion remains unclear. In this study, we found significantly elevated expression of microRNA 21 (miR21), which negatively regulates trophoblast invasion and migration, in preeclamptic placentae. Whole-genome RNA sequencing revealed that PPP2R2B, which encodes PP2A Bß, and the Hippo pathway are downstream targets of miR21. The effects of miR21 on trophoblast mobility were abolished in LATS1T1079A/S909A and YAP-5SA mutants. Moreover, we found that PP2A Bß dephosphorylates LATS1 via direct protein-protein interactions and thus modulates the phosphorylation and subcellular distribution of YAP. PPP2R2B overexpression ameliorated the miR21-induced LATS1-YAP phosphorylation and cytoplasmic sequestration of YAP, which resulted in the rescue of compromised trophoblast invasion and migration. The upregulation of placental miR21 abundance by placenta-specific nanoparticles loaded with agomir-miR21 during placentation interfered with PPP2R2B and activated the Hippo pathway in the placenta, leading to a PE-like phenotype. Thus, aberrant elevation of miR21 impairs EVT mobility by modulating the PP2A Bß/Hippo axis, which is one of the causes of PE.

14.
Opt Lett ; 47(19): 4997-5000, 2022 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-36181170

RESUMO

Mechanical oscillators are widely used in many fields of physics, including ultrahigh precision measurements, gravity experiments, and optical mechanical systems. A sub-gram-scale silicon wafer is suspended by a tungsten wire with a diameter of 8 µm, forming a torsion pendulum to detect the laser radiation pressure. We demonstrate the application of a low-frequency, highly sensitive torsion pendulum for the measurement of light forces. In the feedback cooling state, the system exhibits a force sensitivity at the end of the pendulum close to 0.1 fN, approaches the thermal noise limit, and reaches the detection level of the laser radiation pressure of 60 nW.

15.
Artigo em Inglês | MEDLINE | ID: mdl-36239890

RESUMO

Hyperspectral techniques are promising alternatives to traditional methods of investigating potentially toxic metal(loid) contamination. In this study, hyperspectral technology combined with partial least squares regression (PLSR) and extreme learning machine (ELM) established estimation models to predict the contents of copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), lead (Pb) and tin (Sn) in multi-media environments (mine tailings, soils and sediments) surrounding abandoned mineral processing plants in a typical tin-polymetallic mineral agglomeration in Guangxi Autonomous Region. Four spectral preprocessing methods, Savitzky-Golay (SG) smoothing, continuum removal (CR), first derivative (FD) and continuous wavelet transform (CWT), were used to eliminate noise and highlight spectral features. The optimum combinations of spectral preprocessing and machine learning algorithms were explored, then the estimation models with best accuracy were obtained. CWT and CR were excellent spectral pretreatments for the hyperspectral data regardless of the applied algorithms. The coefficients of determination (R2) of estimation models for the best accuracy of various metals (loid) are as follows: Cu (CWT-ELM:0.85), Zn (CR-PLSR:0.93), As (CWT-ELM: 0.86), Cd (CR-PLSR: 0.89), Pb (CWT-PLSR: 0.75) and Sn (CR-ELM: 0.81). In contrast, ELM models had higher accuracy with R2 > 0.80 (except Cd and Pb). In conclusion, ELM-based spectral estimation models are able to predict metal (loid) concentrations with high accuracy and efficiency, providing a potential new combinatorial approach for estimating toxic metal contamination in multi-media environments.

16.
Front Cardiovasc Med ; 9: 952755, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36061546

RESUMO

Gene therapy has revolutionized the field of medicine, offering new hope for those with common and rare diseases. For nearly three decades, adeno-associated virus (AAV) has shown significant therapeutic benefits in multiple clinical trials, mainly due to its unique replication defects and non-pathogenicity in humans. In the field of cardiovascular disease (CVD), compared with non-viral vectors, lentiviruses, poxviruses, and adenovirus vectors, AAV possesses several advantages, including high security, low immunogenicity, sustainable and stable exogenous gene expression etc., which makes AAV one of the most promising candidates for the treatment of many genetic disorders and hereditary diseases. In this review, we evaluate the current information on the immune responses, transport pathways, and mechanisms of action associated with AAV-based CVD gene therapies and further explore potential optimization strategies to improve the efficiency of AAV transduction for the improved safety and efficiency of CVD treatment. In conclusion, AAV-mediated gene therapy has great potential for development in the cardiovascular system.

17.
Artigo em Inglês | MEDLINE | ID: mdl-36074885

RESUMO

The increased complexity and intelligence of automation systems require the development of intelligent fault diagnosis (IFD) methodologies. By relying on the concept of a suspected space, this study develops explainable data-driven IFD approaches for nonlinear dynamic systems. More specifically, we parameterize nonlinear systems through a generalized kernel representation for system modeling and the associated fault diagnosis. An important result obtained is a unified form of kernel representations, applicable to both unsupervised and supervised learning. More importantly, through a rigorous theoretical analysis, we discover the existence of a bridge (i.e., a bijective mapping) between some supervised and unsupervised learning-based entities. Notably, the designed IFD approaches achieve the same performance with the use of this bridge. In order to have a better understanding of the results obtained, both unsupervised and supervised neural networks are chosen as the learning tools to identify the generalized kernel representations and design the IFD schemes; an invertible neural network is then employed to build the bridge between them. This article is a perspective article, whose contribution lies in proposing and formalizing the fundamental concepts for explainable intelligent learning methods, contributing to system modeling and data-driven IFD designs for nonlinear dynamic systems.

18.
Polymers (Basel) ; 14(18)2022 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-36145946

RESUMO

In temperature sensitive hydrogels, the swelling degree or light transmittance of the gel itself changes with variations in ambient temperature, prompting its wide application in controlled drug release, tissue engineering, and material separation. Considering the amphiphilic structure of ß-cyclodextrin (ß-CD), a cellulose-based supramolecular hydrogel with superior temperature sensitivity was synthesized based on a combination of cellulose and ß-CD as well as the host-guest interaction between ß-CD and polypropylene glycol (PPG). In the one-pot tandem reaction process, chemical grafting of ß-CD on cellulose and the inclusion complexation of ß-CD with PPG were performed simultaneously in a NaOH/urea/water system. The obtained supramolecular hydrogel had a lower critical solution temperature (LCST) of 34 °C. There existed covalent bonding between the cellulose and ß-CD, host-guest complexation between the ß-CD and PPG, and hydrogen bonding and hydrophobic interactions between the components in the network structure of the supramolecular hydrogel. The combination of various covalent and non-covalent bonds endowed the resulting supramolecular hydrogel with good internal network structure stability and thermal stability, as well as sensitive temperature responsiveness within a certain range-implying its potential as a smart material in the fields of medicine, biology, and textiles. This work is expected to bring new strategies for the fabrication of cellulose-based thermosensitive materials, benefitting the high-value utilization of cellulose.

19.
Anal Biochem ; 657: 114906, 2022 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-36152874

RESUMO

A fast and highly sensitive amplified luminescent proximity homogeneous assay (AlphaLISA) method was developed for quantitation of plasma heparin-binding protein levels. In this study, a method directly coupling donor and acceptor beads modified with aldehyde groups to anti-HBP antibodies was proposed, which can effectively simplify the steps and shorten the reaction time to achieve faster detection. Therefore, the developed method required only 15 min of reaction time to generate results. Compared with the approved commercial kit, the developed method had a wider linear range (2.78-500 ng/mL). The excellent linear range means that the method can better exploit the value of HBP in clinical applications. Meanwhile, results of amplified luminescent proximity homogeneous assay and fluorescence dry quantitative immunoassay had good correlation and consistency (ρ = 0.9181). Moreover, the plasma HBP concentrations of patients with bacterial infection were significantly higher than those of healthy individuals (P < 0.0001), indicating the potential applicability of the proposed method for predicting the incidence of bacterial infections. Importantly, the newly developed method is expected to serve as an alternative to the traditional assay method and provides a completely new platform for other biomarkers that require rapid detection.


Assuntos
Proteínas Sanguíneas , Medições Luminescentes , Aldeídos , Peptídeos Catiônicos Antimicrobianos , Humanos , Medições Luminescentes/métodos
20.
Artigo em Inglês | MEDLINE | ID: mdl-36114975

RESUMO

Although hydroxyapatite (HAP) can prominently lower Cd uptake by celery from Cd-polluted soil, its high application rates in reality may lead to high cost and potential environmental risk. Therefore, we aimed to clarify whether combined amendments of HAP and another low-cost material (hydrated lime, corn straw-derived biochar, or zeolite) with reduced application rate of each single amendment could significantly decrease Cd transfer in soil-celery-human system without side effect on celery growth through a pot experiment. Results revealed that adding biochar, HAP, zeolite, or combined amendments had no obvious side effect on celery growth, while adding 0.3% hydrated lime significantly decreased fresh edible celery yield by 69.0%. Conversely, adding 0.5% HAP + 0.05% hydrated lime increased fresh edible celery yield by 39.8%. Additionally, adding HAP, zeolite, or hydrated lime rather than adding biochar effectively decreased total and bioaccessible Cd in edible celery. Similarly, HAP + hydrated lime and HAP + zeolite were much more efficient than HAP + biochar in lowering Cd transfer in soil-celery-human system. The total and bioaccessible Cd in edible celery were even reduced by over 50.0% after adding HAP + hydrated lime or HAP + zeolite at low rates. Considering the effects on celery growth and Cd transfer, HAP + hydrated lime and HAP + zeolite have the potential in remediating soil Cd contamination.

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