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1.
Small ; : e2105387, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34799983

RESUMO

Single-atom catalysts (SACs) are attractive candidates for oxygen reduction reaction (ORR). The catalytic performances of SACs are mainly determined by the surrounding microenvironment of single metal sites. Microenvironment engineering of SACs and understanding of the structure-activity relationship is critical, which remains challenging. Herein, a self-sacrificing strategy is developed to synthesize asymmetric N,S-coordinated single-atom Fe with axial fifth hydroxy (OH) coordination (Fe-N3 S1 OH) embedded in N,S codoped porous carbon nanospheres (FeN/SC). Such unique penta-coordination microenvironment is determined by cutting-edge techonologies aiding of systematic simulations. The as-obtained FeN/SC exhibits superior catalytic ORR activity, and showcases a half-wave potential of 0.882 V surpassing the benchmark Pt/C. Moreover, theoretical calculations confirmed the axial OH in FeN3 S1 OH can optimize 3d orbitals of Fe center to strengthen O2 adsorption and enhance O2 activation on Fe site, thus reducing the ORR barrier and accelerating ORR dynamics. Furthermore, FeN/SC containing H2 O2 fuel cell performs a high peak power density of 512 mW cm-2 , and FeN/SC based Znair batteries show the peak power density of 203 and 49 mW cm-2 in liquid and flexible all-solid-state configurations, respectively. This study offers a new platform for fundamentally understand the axial fifth coordination in asymmetrical planar single-atom metal sites for electrocatalysis.

2.
Nutrients ; 13(8)2021 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-34444660

RESUMO

Dietary protamine can ameliorate hyperlipidemia; however, the protamine-derived active peptide and its hypolipidemic mechanism of action are unclear. Here, we report the discovery of a novel anti-obesity and hypocholesterolemic peptide, RPR (Arg-Pro-Arg), derived from protamine in mice fed a high-fat diet for 50 days. Serum cholesterol levels were significantly lower in the protamine and RPR groups than in the control group. White adipose tissue weight was significantly decreased in the protamine and RPR groups. The fecal excretion of cholesterol and bile acid was significantly higher in the protamine and RPR groups than in the control group. We also observed a significant decrease in the expression of hepatic SCD1, SREBP1, and adipocyte FAS mRNA, and significantly increased expression of hepatic PPARα and adipocyte PPARγ1 mRNA in the protamine group. These findings demonstrate that the anti-obesity effects of protamine are linked to the upregulation of adipocyte PPARγ1 and hepatic PPARα and the downregulation of hepatic SCD1 via SREBP1 and adipocyte FAS. RPR derived from protamine has a crucial role in the anti-obesity action of protamine by evaluating the effective dose of adipose tissue weight loss.


Assuntos
Tecido Adiposo Branco/efeitos dos fármacos , Fármacos Antiobesidade/farmacologia , Anticolesterolemiantes/farmacologia , Colesterol/sangue , Obesidade/tratamento farmacológico , Oligopeptídeos/farmacologia , Protaminas/farmacologia , Tecido Adiposo Branco/metabolismo , Tecido Adiposo Branco/fisiopatologia , Adiposidade/efeitos dos fármacos , Animais , Biomarcadores/sangue , Dieta Hiperlipídica , Modelos Animais de Doenças , Ácido Graxo Sintase Tipo I/genética , Ácido Graxo Sintase Tipo I/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Obesidade/genética , Obesidade/metabolismo , Obesidade/fisiopatologia , PPAR alfa/genética , PPAR alfa/metabolismo , PPAR gama/genética , PPAR gama/metabolismo , Estearoil-CoA Dessaturase/genética , Estearoil-CoA Dessaturase/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo , Perda de Peso/efeitos dos fármacos
3.
Curr Microbiol ; 78(2): 765-774, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33469719

RESUMO

The purpose of this study was to evaluate the control effect of Bacillus atrophaeus TE7 on Cladosporium cladosporioides of mango fruit and how it effects quality attributes during 'Tainong' mango fruit storage. The results showed that strain TE7 had inhibition ability with the biocontrol efficacy of 85.56%. Furthermore, strain TE7 could produce lipopeptide substance, iturin A, and surfactants, which inhibited the growth and development of C. cladosporioides. Moreover, strain TE7 had the ability of improving the activities of defense response-related enzyme in mangoes. The changes of peel color, flesh firmness, contents of total soluble solids (TSS), titratable acid (TA), and ascorbic acid (Vc) were significantly delayed by strain TE7. The results demonstrated that B. atrophaeus TE7 could be applied as a biocontrol agent for the pathogen C. cladosporioides of mango fruit.


Assuntos
Mangifera , Bacillus , Cladosporium , Frutas
4.
Cell Death Dis ; 11(10): 880, 2020 10 20.
Artigo em Inglês | MEDLINE | ID: mdl-33082313

RESUMO

Autophagy is a dynamic circulatory system that occurs in all eukaryotic cells. Cytoplasmic material is transported to lysosomes for degradation and recovery through autophagy. This provides energy and macromolecular precursors for cell renewal and homeostasis. The Hippo-YAP pathway has significant biological properties in controlling organ size, tissue homeostasis, and regeneration. Recently, the Hippo-YAP axis has been extensively referred to as the pathophysiological processes regulating autophagy. Understanding the cellular and molecular basis of these processes is crucial for identifying disease pathogenesis and novel therapeutic targets. Here we review recent findings from Drosophila models to organisms. We particularly emphasize the regulation between Hippo core components and autophagy, which is involved in normal cellular regulation and the pathogenesis of human diseases, and its application to disease treatment.


Assuntos
Autofagia/fisiologia , Proteínas Serina-Treonina Quinases/metabolismo , Animais , Proliferação de Células , Drosophila/metabolismo , Humanos , Camundongos , Camundongos Transgênicos
5.
Int J Biol Sci ; 16(14): 2692-2703, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32792865

RESUMO

Our previous studies have found that human papillomavirus (HPV)-16 E7 oncoprotein promotes epithelial-mesenchymal transition (EMT) and hypoxia-inducible factor-1α (HIF-1α) protein accumulation in non-small cell lung cancer (NSCLC) cells and monoamine oxidase A (MAOA) is highly expressed in NSCLC tissues. Here, we further explored the role of MAOA in HPV-16 E7-induced EMT and HIF-1α protein accumulation in A549 and NCI-H460 NSCLC cells. Our results showed that HPV-16 E7 enhanced MAOA expression in NSCLC cells. Additionally, MAOA knockout inhibited HPV-16 E7-induced migration, invasion, and EMT, and significantly reduced HPV-16 E7-induced ROS generation and HIF-1α protein accumulation via promoting its degradation. Furthermore, MAOA knockout suppressed HPV-16 E7-induced ERK1/2 activation. In vivo, MAOA knockout inhibited tumor growth, metastasis, and the expression of EMT-related markers and HIF-1α proteins induced by HPV-16 E7 in NCI-H460 NSCLC subcutaneous xenograft and in situ intrapulmonary models of nude mice. Taken together, our findings provide evidence that MAOA plays a key role in EMT and HIF-1α protein accumulation induced by HPV-16 E7 in NSCLC cells, suggesting that MAOA may be a potential therapeutic target for HPV-related NSCLC.

6.
Biomed Pharmacother ; 127: 110209, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32559848

RESUMO

Genome regions that do not for code for proteins are generally transcribed into long non-coding RNAs. Growing evidence reveals that lncRNAs, defined as transcripts longer than 200 nucleotides, are commonly deregulated in cervical malignancies. New sequencing technologies have revealed a complete picture of the composition of the human transcriptome. LncRNAs perform diverse functions at transcriptional, translation, and post-translational levels through interactions with proteins, RNA and DNA. In the past decade, studies have shown that lncRNAs participate in the pathogenesis of many diseases, including cervical cancer. Hence, illuminating the roles of lncRNA will improve our understanding of cervical cancer. In this work, we summarize the current knowledge on lncRNAs in cervical cancer. We describe the emerging roles of lncRNAs in cervical cancer, particularly in cancer progression, metastasis, treatment resistance, HPV regulation, and metabolic reprogramming. The great promises of lncRNAs as potential biomarkers for cervical cancer diagnosis and prognosis are also discussed. We discuss current technologies used to target lncRNAs and thus control cancers, such as antisense oligonucleotides, CRISPR-Cas9, and exosomes. Overall, we show that lncRNAs hold great potentials as therapeutic agents and innovative biomarkers. Finally, further clinical research is necessary to advance our understanding of the therapeutic value of lncRNAs in cervical cancer.


Assuntos
Regulação Neoplásica da Expressão Gênica/fisiologia , Terapia de Alvo Molecular/métodos , RNA Longo não Codificante/fisiologia , Neoplasias do Colo do Útero/genética , Neoplasias do Colo do Útero/fisiopatologia , Feminino , Humanos , Neoplasias do Colo do Útero/diagnóstico
7.
Small ; 16(10): e1906735, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31984632

RESUMO

The development of rechargeable metal-air batteries and water electrolyzers are highly constrained by electrocatalysts for the oxygen reduction reaction (ORR), oxygen evolution reaction (OER), and hydrogen evolution reaction (HER). However, the construction of efficient trifunctional electrocatalysts for ORR/OER/HER are highly desirable yet challenging. Herein, hollow carbon nanotubes integrated single cobalt atoms with Co9 S8 nanoparticles (CoSA + Co9 S8 /HCNT) are fabricated by a straightforward in situ self-sacrificing strategy. The structure of the CoSA + Co9 S8 /HCNT are verified by X-ray absorption spectroscopy and aberration-corrected scanning transmission electron microscopy. Theoretical calculations and experimental results embrace the synergistic effects between Co9 S8 nanoparticles and single cobalt atoms through optimizing the electronic configuration of the CoN4 active sites to lower the reaction barrier and facilitating the ORR, OER, and HER simultaneously. Consequently, rechargeable liquid and all-solid-state flexible Zn-air batteries based on CoSA + Co9 S8 /HCNT exhibit remarkable stability and excellent power density of 177.33 and 51.85 mW cm-2 , respectively, better than Pt/C + RuO2 counterparts. Moreover, the as-fabricated Zn-air batteries can drive an overall water splitting device assembled with CoSA + Co9 S8 /HCNT and achieve a current density of 10 mA cm-2 at a low voltage of 1.59 V, also superior to Pt/C + RuO2 . Therefore, this work presents a promising approach to an efficient trifunctional electrocatalyst toward practical applications.

8.
Front Oncol ; 10: 593085, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33614479

RESUMO

Cancer has been a daunting challenge for human beings because of its clonal heterogeneity and compositional complexity. Tumors are composed of cancer cells and a variety of non-cancer cells, which together with the extracellular matrix form the tumor microenvironment. These cancer-related cells and components and immune mechanisms can affect the development and progression of cancer and are associated with patient diagnosis, treatment and prognosis. As the first choice for the study of complex biological systems, single-cell transcriptional sequencing (scRNA-seq) has been widely used in cancer research. ScRNA-seq has made breakthrough discoveries in tumor heterogeneity, tumor evolution, metastasis and spread, development of chemoresistance, and the relationship between the tumor microenvironment and the immune system. These results will guide clinical cancer treatment and promote personalized and highly accurate cancer treatment. In this paper, we summarize the latest research progress of scRNA-seq and its guiding significance for clinical treatment.

9.
J Cancer ; 10(9): 1997-2005, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31205560

RESUMO

Our previous study has demonstrated that cytochalasin H (CyH) isolated from mangrove-derived endophytic fungus induces apoptosis and inhibits migration in A549 non-small cell lung cancer (NSCLC) cells. In this study, we further explored the effect of CyH on angiogenesis in NSCLC cells and the underlying molecular mechanisms. A549 and H460 NSCLC cells were treated with different concentrations of CyH for 24 h. The effects of CyH on NSCLC angiogenesis in vitro and in vivo were investigated. Hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression in xenografted NSCLC of nude mice was analyzed by immunohistochemistry. ELISA was used to analyze the concentration of VEGF in the conditioned media derived from treated and untreated NSCLC cells. Western blot was performed to detect the levels of HIF-1α, p-AKT, p-P70S6K, and p-ERK1/2 proteins, and RT-qPCR was used to determine the levels of HIF-1α and VEGF mRNA in A549 and H460 cells. Our results showed that CyH significantly inhibited angiogenesis in vitro and in vivo, and suppressed the hemoglobin content and HIF-1α and VEGF protein expression in xenografted NSCLC tissues of nude mice. Meanwhile, CyH inhibited the secretion of VEGF protein and the expression of HIF-1α protein in A549 and H460 cells. Moreover, CyH had a significant inhibitory effect on VEGF mRNA expression but had no effect on HIF-1α mRNA expression, and CyH inhibited HIF-1α protein expression by promoting the degradation of HIF-1α protein in A549 and H460 cells. Additionally, CyH dramatically inhibited AKT, P70S6K, and ERK1/2 activation in A549 and H460 cells. Taken together, our results suggest that CyH can inhibit NSCLC angiogenesis by the suppression of HIF-1α protein accumulation and VEGF expression through PI3K/AKT/P70S6K and ERK1/2 signaling pathways.

10.
J Cancer ; 9(19): 3456-3466, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30310502

RESUMO

Background: Our previous studies have demonstrated that human papillomaviruse (HPV)-16 oncoproteins promoted epithelial-mesenchymal transition (EMT), leading to non-small cell lung cancer (NSCLC) progression, but the underlying molecular mechanisms still remain unclear. PI3K/Akt/HIF-1α signaling pathway has been reported to mediate hypoxia-induced EMT. In this study, we further explored the role of PI3K/Akt/HIF-1α signaling pathway in HPV-16 oncoprotein-induced EMT in NSCLC cells. Methods: A549 and NCI-H460 NSCLC cells were transiently transfected with pEGFP-HPV-16 E6 or E7 constructs. Western blotting and RT-qPCR were respectively performed to determine the protein and mRNA expression of EMT-related transcription factors. HPV-16 E6 or E7-transfected NSCLC cells were co-transfected with specific HIF-1α-siRNA or pretreated with different concentrations of LY294002, a specific PI3K inhibitor, followed by the analysis of expression of EMT-related transcription factors. The correlation between HIF-1α and EMT-related transcription factors in NSCLC tissues was analyzed by immunohistochemical staining and Spearman rank correlation coefficient. Results: HPV-16 E6 and E7 oncoproteins upregulated the expression of Slug and Twist1, the EMT-related transcription factors, at both protein and mRNA levels in A549 and NCI-H460 cells. The co-transfection with specific HIF-1α-siRNA, but not the non-specific (NS)-siRNA, significantly abrogated HPV-16 oncoprotein-induced upregulation of ZEB1, Snail1, Slug, and Twist1 at both protein and mRNA levels. Additionally, pretreatment with LY294002 obviously blocked HPV-16 E6- and E7-induced Snail1, Slug, and Twist1 protein expression in A549 and NCI-H460 cells. Further analysis of clinical specimens showed that HIF-1α protein was strongly expressed in NSCLC tissues, which was positively correlated with ZEB1, Snail1, Slug, and Twist1 protein expression. Conclusions: PI3K/Akt/HIF-1α may contribute to the progression of HPV-associated NSCLC via mediating the expression of EMT-related transcription factors in NSCLC cells.

11.
Oncol Rep ; 39(6): 2899-2905, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29620283

RESUMO

Cytochalasin H (CyH) has been shown to exhibit promising anticancer activities against various types of cancers; however, the underlying mechanisms remain unknown. In a previous study, we isolated CyH from the mangrove­derived endophytic fungus Phomopsis sp. in Zhanjiang, China. In the present study, we further explored the effect of CyH on apoptosis and migration in the human lung adenocarcinoma cell line A549. Cell Counting kit­8 (CCK­8) assay was used to observe the effects of CyH on the growth of A549 cells. The cell cycle and apoptosis were determined using flow cytometry. The effect of CyH on cell migration was observed by scratch wound healing and chamber migration assays. Western blotting was used to detect the expression of apoptosis­ and metastasis­associated proteins. Our results showed that CyH exhibited cytotoxicity to A549 cells. The treatment of CyH arrested A549 cells at the G2/M phase. Furthermore, sub­G1 peaks and fragmented DNA ladders were observed, and the mitochondrial transmembrane potential was also decreased in CyH­treated A549 cells. CyH significantly increased Bax, P53, and cleaved caspase­3 (17 kDa) protein expression and decreased Bcl­xL, Bcl­2, and full­length caspase­3 (35 kDa) protein expression, resulting in an increased ratio of the pro­apoptosis/anti­apoptosis proteins Bax/Bcl­2. Additionally, CyH treatment inhibited the migration ability of A549 cells in a dose­dependent manner. Taken together, our results suggest that CyH may be a potential chemopreventive drug for the treatment of lung cancer.


Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Citocalasinas/farmacologia , Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos , Neoplasias Pulmonares/metabolismo , Saccharomycetales/química , Células A549 , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citocalasinas/química , Citocalasinas/isolamento & purificação , Ensaios de Seleção de Medicamentos Antitumorais , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Áreas Alagadas
12.
Oncol Lett ; 15(3): 3245-3251, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29435065

RESUMO

Monoamine oxidase A (MAOA), a mitochondrial enzyme, is closely associated with neurological disorders. Recently, MAOA has been linked to the progression of prostate cancer, hepatocellular carcinoma, and cholangiocarcinoma. However, MAOA was reported to have different effects on the progression of these types of cancer, and the role of MAOA in non-small cell lung cancer (NSCLC) progression remains unclear. The present study determined the expression of MAOA and epithelial to mesenchymal transition (EMT) markers in 45 pairs of NSCLC and matched non-tumor adjacent lung tissues, and further analyzed the correlation between MAOA expression and the EMT or the development of clinicopathological features. The results demonstrated that protein and mRNA expression levels of MAOA in NSCLC tissues were higher than those observed in the matched non-tumor adjacent lung tissues. Furthermore, the increased MAOA expression in NSCLC tissues was positively correlated with N-cadherin (r=0.525, P=0.002), Slug (r=0.515, P=0.001), and Twist (r=0.448, P=0.008) expressions, but negatively correlated with E-cadherin expression (r=-0.387, P=0.01). Additionally, the elevated MAOA expression in NSCLC tissues was associated with late stage NSCLC (Z=-2.596, P=0.029) and lymph node metastases (Z=-2.378, P=0.020). These findings suggest that MAOA may have a role in promoting NSCLC progression by mediating EMT.

13.
Front Plant Sci ; 8: 913, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28674540

RESUMO

Phospholipase D (PLD) in plants plays vital roles in growth, development, and stress responses. However, the precise role of PLDs in pineapple remains poorly understood. In this study, 10 putative PLD genes, designated as AcPLD1-AcPLD10, were identified based on the pineapple genome database. The 10 AcPLDs could be clustered into five of the six known PLD families according to sequence characterization. Their deduced amino acid sequences displayed similarities to PLDs from other plant species. Expression analyses of PLD mRNAs from pineapple pulp were performed. The 10 PLDs exhibited differential expression patterns during storage periods of fruits treated with hexaldehyde (a specific PLD inhibitor) which could alleviate internal browning (IB) of pineapple after harvest. Functional subcellular localization signaling assays of two PLD proteins (AcPLD2 and AcPLD9) were performed by fluorescence microscopy. To further detect the potential action mechanism underlying PLD involved in the IB defense response, PLD, hydrogen peroxide (H2O2) and H2O2 associated with antioxidative enzymes such as superoxide dismutase, catalase, NADPH, and ascorbate peroxidase were quantified by enzyme-linked immunosorbent assay. This report is the first to provide a genome-wide description of the pineapple PLD gene family, and the results should expand knowledge of this family.

14.
Oncol Res ; 25(5): 843-852, 2017 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-28508744

RESUMO

The human papillomavirus (HPV) infection may be associated with the development and progression of non-small cell lung cancer (NSCLC). However, the role of HPV-16 oncoproteins in the development and progression of NSCLC is not completely clear. Epithelial-mesenchymal transition (EMT), a crucial step for invasion and metastasis, plays a key role in the development and progression of NSCLC. Here we explored the effect of HPV-16 oncoproteins on EMT and the underlying mechanisms. NSCLC cell lines, A549 and NCI-H460, were transiently transfected with the EGFP-N1-HPV-16 E6 or E7 plasmid. Real-time PCR and Western blot analysis were performed to analyze the expression of EMT markers. A protein microarray was used to screen the involved signaling pathway. Our results showed that overexpression of HPV-16 E6 and E7 oncoproteins in NSCLC cells significantly promoted EMT-like morphologic changes, downregulated the mRNA and protein levels of EMT epithelial markers (E-cadherin and ZO-1), and upregulated the mRNA and protein levels of EMT mesenchymal markers (N-cadherin and vimentin) and transcription factors (ZEB-1 and Snail-1). Furthermore, the HPV-16 E6 oncoprotein promoted STAT3 activation. Moreover, WP1066, a specific signal transducer and activator of transcription 3 (STAT3) inhibitor, reversed the effect of HPV-16 E6 on the expression of ZO-1, vimentin, and ZEB-1 in transfected NSCLC cells. Taken together, our results suggest that overexpression of HPV-16 E6 and E7 oncoproteins enhances EMT, and the STAT3 signaling pathway may be involved in HPV-16 E6-induced EMT in NSCLC cells.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/etiologia , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Transição Epitelial-Mesenquimal , Papillomavirus Humano 16/genética , Neoplasias Pulmonares/etiologia , Neoplasias Pulmonares/metabolismo , Infecções por Papillomavirus/complicações , Fator de Transcrição STAT3/metabolismo , Carcinoma Pulmonar de Células não Pequenas/patologia , Linhagem Celular Tumoral , Transformação Celular Viral , Humanos , Neoplasias Pulmonares/patologia , Proteínas Oncogênicas Virais/genética , Proteínas E7 de Papillomavirus/genética , Proteínas Repressoras/genética , Transdução de Sinais
15.
Eur J Med Genet ; 53(6): 347-57, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20624502

RESUMO

Congenital cataracts are a major cause of induced blindness in children, and inherited cataracts are the major cause of congenital cataracts. Inherited congenital cataracts have been associated with mutations in specific genes, including those of crystallins, gap junction proteins, membrane transport and channel proteins, the cytoskeleton, and growth and transcription factors. Locating and identifying the genes and mutations involved in cataractogenesis are essential to gaining an understanding of the molecular defects and pathophysiologic characteristics of inherited congenital cataracts. In this review, we summarize the current research in this field.


Assuntos
Catarata/congênito , Catarata/genética , Conexinas/genética , Cristalinas/genética , Citoesqueleto/genética , Animais , Aquaporinas/genética , Aquaporinas/metabolismo , Criança , Conexinas/metabolismo , Citoesqueleto/metabolismo , Proteínas do Olho/genética , Proteínas do Olho/metabolismo , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Humanos , Proteínas de Filamentos Intermediários/genética , Proteínas de Filamentos Intermediários/metabolismo , Cristalino/fisiologia , Camundongos , Camundongos Knockout , Mutação , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados/genética , Fatores de Transcrição Box Pareados/metabolismo , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Vimentina/genética , Vimentina/metabolismo
16.
J Reprod Immunol ; 75(1): 40-7, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17418903

RESUMO

Experimental autoimmune ovarian disease (AOD) is a T cell-mediated chronic inflammatory disease that may lead to premature ovarian failure. Autoimmune disease can be suppressed by oral administration of autoantigens leading to tolerance. One of the major mechanisms of oral tolerance is induction of regulatory CD4+ T cells that can mediate active suppression by producing immunomodulatory cytokines. However, the role of oral tolerance as a treatment for experimental AOD has received little attention. Therefore, the purpose of this study was to examine the conditions necessary to produce oral tolerance in experimental AOD in B6AF1 female mice. In this study, mice received different doses of peptides of the mouse zona pellucida 3 (pZP3) via gastric intubation for 7 times. After 4 times of oral administration, AOD was induced by immunization with pZP3. The optimal tolerating regimen for oral administration of pZP3 in mice was 10 microg, which decreased morbidity of oophoritis compared to the control group. In this moderate-dose therapeutic group (MD), alterations in the estrous cycle were normalized and CD4+ T cells that were CD25+ increased while those that were CD25- decreased. The severity of autoimmune oophoritis and the titer of ZP autoantibodies were also significantly reduced. These findings suggest that oral administration of pZP3 may be successfully used as an oral tolerance strategy for suppression of AOD.


Assuntos
Autoantígenos/imunologia , Doenças Autoimunes/imunologia , Linfócitos T CD4-Positivos/imunologia , Proteínas do Ovo/imunologia , Glicoproteínas de Membrana/imunologia , Ooforite/imunologia , Peptídeos/administração & dosagem , Peptídeos/imunologia , Receptores de Superfície Celular/imunologia , Administração Oral , Animais , Autoanticorpos/sangue , Doenças Autoimunes/metabolismo , Linfócitos T CD4-Positivos/metabolismo , Modelos Animais de Doenças , Proteínas do Ovo/administração & dosagem , Estro , Feminino , Tolerância Imunológica , Glicoproteínas de Membrana/administração & dosagem , Camundongos , Camundongos Endogâmicos C57BL , Receptores de Superfície Celular/administração & dosagem , Glicoproteínas da Zona Pelúcida
17.
Zhonghua Yi Xue Za Zhi ; 82(3): 164-7, 2002 Feb 10.
Artigo em Chinês | MEDLINE | ID: mdl-11953151

RESUMO

OBJECTIVE: To investigate the incidence of twin and twin with birth defect (BD) and with neural tube defect (NTD) in China. METHODS: A prospective monitoring was conducted among the perinatal infants born in 945 hospitals in 20 provinces/municipalities/autonomous regions in China during the period from 1 October 1986 to 30 September 1987 to count up the numbers of twins and of twins with BD or NTD. RESULTS: 1 243 284 infants were born during this period. Among these perinatal infants there were 12 715 pairs of twins with an incidence of 10.23%. The incidence rates of twin with BD and of twin with NTD were 36.81% and 5.27% respectively. There was no difference in incidence rate of twin among different parts of the country and between urban area and rural area. The incidence rate of twin was the highest among the women aged 20 approximately 39, among primipara, and in October and November. The incidence rate of twin with BD showed no difference among different seasons, parities, and parts of the country and between urban area and rural area. Most of twin with BD occurred at the age 20 approximately 39. Most twins with NTD were born in the northern provinces and rural area. The incidence rate of twin with NTD was correlated with season, most twins with NTD being born in November, and was no correlated with age and parity of the mother. CONCLUSION: The incidence of BD, especially the incidence of NTD, among twins is significantly higher than that among total perinatal infants in China. Prenatal monitoring is important for twin pregnancy.


Assuntos
Anormalidades Congênitas/epidemiologia , Defeitos do Tubo Neural/epidemiologia , Gêmeos/estatística & dados numéricos , Ordem de Nascimento , China/epidemiologia , Anormalidades Congênitas/classificação , Feminino , Humanos , Recém-Nascido , Masculino , Idade Materna , Estações do Ano , Estudos em Gêmeos como Assunto/métodos , Estudos em Gêmeos como Assunto/estatística & dados numéricos
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