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1.
Front Med (Lausanne) ; 9: 887354, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35665339

RESUMO

Sjögren's syndrome (SS) is a systemic autoimmune disease with no efficient treatment, and it is associated with dysregulated immune cells and impaired interleukin (IL)-2 signaling. IL-2 is critical for the development and maintenance of Treg cells. The use of low dose of IL-2 (LDIL-2) in the treatment of autoimmune diseases is promising, but the efficacy and mechanism in SS therapy are still to be confirmed. This study aims to investigate the therapeutic effect of LDIL-2 on SS in NOD (non-obese diabetic) mice. NOD mice (female, 8 weeks old) were randomly assigned into three groups (n = 8). Low dose of IL-2 (LDIL-2), high dose of IL-2 (HDIL-2), and isometric sterile water (control) were administered subcutaneously daily from week 8 to week 16. LDIL-2 administration significantly recovered the reduction in saliva flow and suppressed lymphocyte inflammation of the submandibular glands (SMGs) when compared with those treated with sterile water as controls (p < 0.05). SS related biomarkers including ANA, Anti-SSA/Ro, and Anti-SSB/La also declined (p < 0.05). In the low dose of IL-2 treated group, the proportion of CD4+CD25+Foxp3+Tregs in both spleen and cervical-lymph-node were higher than control mice (p < 0.05). Furthermore, CD4+Bcl-6+PD-1+CXCR5+Tfh cells, CD4+IFN-γ+Th1 cells, and CD4+IL-17A+Th17 cells were significantly reduced in LDIL-2 group (p < 0.05). Analysis of the SMGs biopsies showed significantly decreased inflammation scores after LDIL-2 administration and an increase of Tregs with immunohistochemical staining. Our findings provide in vivo evidence that LDIL-2 was an effective therapeutic intervention for SS observed in NOD mice and may restore immune balance through the promotion of Treg and suppression of germinal center (GC) B cells and effector T cells.

2.
Cell Mol Biol Lett ; 27(1): 46, 2022 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-35690719

RESUMO

The submandibular gland (SMG) and the sublingual gland (SLG) are two of the three major salivary glands in mammals. In mice, they are adjacent to each other and open into the oral cavity, producing saliva to lubricate the mouth and aid in food digestion. Though salivary gland dysfunction accompanied with fibrosis and metabolic disturbance is common in clinic, in-depth mechanistic research is lacking. Currently, research on how to rescue salivary function is challenging, as it must resort to using terminally differentiated acinar cells or precursor acinar cells with unknown differentiation. In this study, we established reversely immortalized mouse primary SMG cells (iSMGCs) and SLG cells (iSLGCs) on the first postnatal day (P0). The iSMGCs and iSLGCs grew well, exhibited many salivary gland characteristics, and retained the metabolism-related genes derived from the original tissue as demonstrated using transcriptome sequencing (RNA-seq) analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of these two cell lines, which overlapped with those of the SMG and SLG, were enriched in cysteine and methionine metabolism. Furthermore, we investigated the role of bone morphogenetic protein 9 (BMP9), also known as growth differentiation factor 2(Gdf2), on metabolic and fibrotic functions in the SMG and SLG. We demonstrated that iSMGCs and iSLGCs presented promising adipogenic and fibrotic responses upon BMP9/Gdf2 stimulation. Thus, our findings indicate that iSMGCs and iSLGCs faithfully reproduce characteristics of SMG and SLG cells and present a promising prospect for use in future study of salivary gland metabolism and fibrosis upon BMP9/Gdf2 stimulation.


Assuntos
Fator 2 de Diferenciação de Crescimento , Glândula Sublingual , Animais , Linhagem Celular , Fibrose , Fator 2 de Diferenciação de Crescimento/metabolismo , Mamíferos , Camundongos , Glândulas Salivares/metabolismo , Glândula Sublingual/metabolismo
3.
Mol Med Rep ; 26(2)2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35674190

RESUMO

Circular RNAs (circRNAs) are novel RNA transcripts that participate in cancer development. Nonetheless, in colorectal cancer (CRC), the information ~circRNA expression and function is largely unknown. The present study aimed to investigate the expression, function and underlying mechanism of circ_0006174 in CRC. Reverse transcription­quantitative PCR analysis was performed to detect circ_0006174, miR­1205 and calcium­binding epidermal growth factor domain­containing protein 1 (CCBE1) expression levels in CRC tissues and cell lines. Circ_0006174 knockdown CRC cell models were established. CCK­8, TUNEL and Transwell methods were utilized to explore the function of circ_0006174 on the malignant phenotype of CRC cells. Moreover, a xenograft nude mouse model was constructed to verify the effects of circ_0006174 on lung metastasis in vivo. Dual­luciferase reporter gene assay was adopted to prove the association between circ_0006174 and miR­1205, miR­1205 and CCBE1. Gene set enrichment analysis was performed using the LinkedOmics database. Western blotting was performed to evaluate the expression of CCBE1, Ki67 and Wnt pathway­related proteins (c­Myc and cyclin D1) in CRC cell lines. Circ_0006174 showed a notable upregulation in CRC tissues and cell lines and its overexpression was linked to larger tumor diameter and advanced T stage of CRC patients. Circ_0006174 knockdown significantly suppressed cell growth and metastatic potential and promoted cell apoptosis in vitro. Circ_0006174 knockdown accelerated the lung metastasis in vivo. Mechanistically, circ_0006174 could decoy miR­1205 to up­modulate CCBE1 expression and Wnt pathway­related proteins (c­Myc and cyclin D1). Circ_0006174 is an oncogenic circRNA, which participates in the promotion of CRC progression by regulating the miR­1205/CCBE1/Wnt pathway.


Assuntos
Neoplasias Colorretais , Neoplasias Pulmonares , MicroRNAs , Animais , Proteínas de Ligação ao Cálcio/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/genética , Neoplasias Colorretais/patologia , Ciclina D1/metabolismo , Humanos , Camundongos , MicroRNAs/genética , MicroRNAs/metabolismo , RNA Circular/genética , Proteínas Supressoras de Tumor/metabolismo , Via de Sinalização Wnt/genética
4.
Zhongguo Dang Dai Er Ke Za Zhi ; 24(5): 536-542, 2022 May 15.
Artigo em Chinês | MEDLINE | ID: mdl-35644194

RESUMO

OBJECTIVES: To study the features of intestinal flora in children with food protein-induced proctocolitis (FPIP) by high-throughput sequencing. METHODS: A total of 31 children, aged <6 months, who experienced FPIP after exclusive breastfeeding and attended the outpatient service of the Third Affiliated Hospital of Zunyi Medical University from October 2018 to February 2021 were enrolled as the FPIP group. Thirty-one healthy infants were enrolled as the control group. Fecal samples were collected to extract DNA for PCR amplification. High-throughput sequencing was used to perform a bioinformatics analysis of 16S rDNA V3-V4 fragments in fecal samples. RESULTS: The diversity analysis of intestinal flora showed that compared with the control group, the FPIP group had a lower Shannon index for diversity (P>0.05) and a significantly higher Chao index for abundance (P<0.01). At the phylum level, the intestinal flora in both groups were composed of Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Compared with the control group, the FPIP group had a significant reduction in the composition ratio of Actinobacteria (P<0.001) and a significant increase in the composition ratio of Proteobacteria (P<0.05). At the genus level, the intestinal flora in the FPIP group were mainly composed of Escherichia, Clostridium, Enterococcus, Klebsiella, and Bifidobacterium, and the intestinal flora in the control group were mainly composed of Bifidobacterium and Streptococcus. Compared with the control group, the FPIP group had a significant reduction in the composition ratio of Bifidobacterium and Ruminococcus (P<0.05) and significant increases in the composition ratios of Clostridium and Shigella (P<0.05). CONCLUSIONS: Compared with the control group, the FPIP group has a reduction in the diversity of intestinal flora and an increase in their abundance, and there are certain differences in several bacterial genera. These results suggest that changes in the composition of intestinal flora at genus level may play an important role in the development and progression of FPIP.


Assuntos
Microbioma Gastrointestinal , Proctocolite , Bactérias/genética , Bifidobacterium/genética , Criança , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Humanos , Lactente , RNA Ribossômico 16S/genética
5.
Ying Yong Sheng Tai Xue Bao ; 33(6): 1669-1678, 2022 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-35729146

RESUMO

In order to solve the problem that soil soluble phosphorus content in most cultivated land in China is insufficient and the plant growth is inhibited, a phosphate solubilizing microorganism (PB) was screened and identified, and its phosphate solubilizing performance was optimized. The results showed that the PB strain was belonged to Burkholderia stabilis. It had the ability of nitrogen fixation and indole-3-acetic acid (IAA) secretion, as well as a certain inhibitory effect on Escherichia coli. It could maintain high activity and phosphorus solubilizing ability at pH 8.0-10.0, indicating good alkali resistance. The results of phosphorus dissolving performance optimization showed that the phosphate solubilizing capacity of strain PB reached the best at 30℃, pH 7.0, 180 r·min-1, using glucose as carbon source, ammonium sulfate as nitrogen source, tricalcium phosphate as phosphorus source and adding 50 µmol·L-1 lysine. The amount of dissolved phosphorus was 569.33 mg·L-1, which was 1.9 times of that before optimization. The strain mainly secreted citric acid, malonic acid, and glucuronic acid during metabolism. After adding lysine, the type of organic acids secreted by the strain did not change, but the content increased significantly. Results from pot experiments showed that the application of PB bacterial fertilizer could significantly improve the growth and physiological indicators of garlic seedlings, and that the promotion effect was more obvious after adding lysine. Compared with the control, the height of seedling was increased by 18.6%, seedling diameter was increased by 16.7%, aboveground fresh and dry weight were increased by 22.1% and 15.7%, and belowground fresh and dry weight were increased by 22.0% and 28.7%, respectively in PB with lysine treatment. Soil available phosphorus content was 2.1 and 2.3 times of the control in PB and PB+lysine treatments, indicating that PB could improve soil available phosphate content. Adding lysine could strengthen such function.

6.
EClinicalMedicine ; 48: 101464, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35706492

RESUMO

Background: The lack of a comprehensive understanding of the role of mass media in perpetuating weight stigma hinders policy formulation. We reviewed the influence of mass media on weight stigmatisation and the effectiveness of media-based interventions designed to prevent or reduce stigma. Methods: We conducted a systematic review across seven databases from inception to December 2021. Included studies assessed exposure to or impact of weight stigma in mass media or examined interventions to reduce stigma through media in populations 12+ years. We synthesised data narratively, categorising studies based on similarity in focus to produce a set of integrated findings. The systematic review is registered in PROSPERO (No. CRD42020176306). Findings: One-hundred-and-thirteen records were eligible for inclusion from 2402 identified; 95 examined the prevalence of stigmatising content in mass media and its impact on stigma. Weight stigma was prevalent across media types, with the dominant discourse viewing overweight and obesity as an individual responsibility and overlooking systemic factors. Exposure to stigmatising content was found to negatively influence attitudes towards people with overweight or obesity. Few studies considered methods of reducing stigma in the media, with only two testing media-based interventions; their results were promising but limited. Interpretation: Weight stigma in media content is prevalent and harmful, but there is little guidance on reducing it. Future research focus needs to shift from assessing prevalence and impacts to weight stigma interventions. Funding: None.

7.
RSC Adv ; 12(23): 14477-14484, 2022 May 12.
Artigo em Inglês | MEDLINE | ID: mdl-35702243

RESUMO

A novel series of picoline-based ionic liquid surfactants, N-alkyoxycarbonyl-3-picoline bromides [C n Empy][Br] (n = 10, 12, 14), have been synthesized. The thermal stability, aggregation behavior and surface activity of the synthetic ionic liquid surfactants were investigated systematically though a series of methods, such as thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), tensiometry and conductivity. The thermodynamics of micellization of the ionic liquid surfactants solution were studied by using the conductivity method in the temperature range 278.15-318.15 K. The surface activity parameters and thermodynamics parameters were derived, respectively. The enthalpy-entropy compensation effect was further discussed by using relative thermodynamics parameters. It was found that the [C n Empy][Br] have moderate surface activity, and their critical micelle concentration (CMC) decreased with the ester-functionalized chain length and exhibited a U-shape with temperature. The calculation results of the thermodynamic parameters showed that the micellization processes of [C n Empy][Br] were spontaneous, endothermic at low temperature and exothermic at higher temperature.

8.
Front Chem ; 10: 890925, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35711955

RESUMO

The development of heteronuclear metal complexes as potent anticancer agents has received increasing attention in recent years. In this study, two new heteronuclear Ru(Ⅱ)-Re(Ⅰ) metal complexes, [Ru(bpy)2LRe(CO)3(DIP)](PF6)3 and [Ru(phen)2LRe(CO)3(DIP)](PF6)3 [RuRe-1 and RuRe-2, L = 2-(4-pyridinyl)imidazolio[4,5-f][1,10]phenanthroline, bpy = 2,2'-bipyridine, DIP = 4,7-diphenyl-1,10-phenanthroline, phen = 1,10-phenanthroline], were synthesized and characterized. Cytotoxicity assay shows that RuRe-1 and RuRe-2 exhibit higher anticancer activity than cisplatin, and exist certain selectivity toward human cancer cells over normal cells. The anticancer mechanistic studies reveal that RuRe-1 and RuRe-2 can induce apoptosis through the regulation of cell cycle, depolarization of mitochondrial membrane potential (MMP), elevation of intracellular reactive oxygen species (ROS), and caspase cascade. Moreover, RuRe-1 and RuRe-2 can effectively inhibit cell migration and colony formation. Taken together, heteronuclear Ru(Ⅱ)-Re(Ⅰ) metal complexes possess the prospect of developing new anticancer agents with high efficacy.

9.
Nature ; 605(7909): 262-267, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-35546188

RESUMO

The scaling of silicon metal-oxide-semiconductor field-effect transistors has followed Moore's law for decades, but the physical thinning of silicon at sub-ten-nanometre technology nodes introduces issues such as leakage currents1. Two-dimensional (2D) layered semiconductors, with an atomic thickness that allows superior gate-field penetration, are of interest as channel materials for future transistors2,3. However, the integration of high-dielectric-constant (κ) materials with 2D materials, while scaling their capacitance equivalent thickness (CET), has proved challenging. Here we explore transferrable ultrahigh-κ single-crystalline perovskite strontium-titanium-oxide membranes as a gate dielectric for 2D field-effect transistors. Our perovskite membranes exhibit a desirable sub-one-nanometre CET with a low leakage current (less than 10-2 amperes per square centimetre at 2.5 megavolts per centimetre). We find that the van der Waals gap between strontium-titanium-oxide dielectrics and 2D semiconductors mitigates the unfavourable fringing-induced barrier-lowering effect resulting from the use of ultrahigh-κ dielectrics4. Typical short-channel transistors made of scalable molybdenum-disulfide films by chemical vapour deposition and strontium-titanium-oxide dielectrics exhibit steep subthreshold swings down to about 70 millivolts per decade and on/off current ratios up to 107, which matches the low-power specifications suggested by the latest International Roadmap for Devices and Systems5.

10.
Brain Res Bull ; 185: 91-98, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35550155

RESUMO

Major depressive disorder (MDD) is one of the most debilitating and severe mental diseases globally. Increasing evidence has shown that epigenetics is critical for understanding brain function and brain disorders, including MDD. N-acetyltransferase 10 (NAT10), acting on histones, mRNA and other substrates, has been reported to be involved in epigenetic events, including histone acetylation and mRNA modifications. NAT10 is highly expressed in the brain. However, the potential effects of NAT10 on MDD are still unknown. Here, we exploited chronic mild stress (CMS) to induce anxiety- and depression-like behaviors in mice and found that the expression of NAT10 in the mouse hippocampus was upregulated after CMS treatment. Inhibition of NAT10 by pharmacological methods produced anxiolytic- and antidepressant-like effects. Neuron-specific overexpression of NAT10 in the hippocampus resulted in anxiety- and depression-like behaviors, accompanied by higher SIRT1 protein levels, and lower dendritic spine densities. Overall, it was found that elevation of NAT10 in hippocampal neurons is involved in the occurrence of anxiety- and depression-like behaviors, suggesting that NAT10 could be a potential new target for developing anxiolytics and antidepressants.


Assuntos
Depressão , Transtorno Depressivo Maior , Acetiltransferases/metabolismo , Acetiltransferases/farmacologia , Acetiltransferases/uso terapêutico , Animais , Antidepressivos/uso terapêutico , Ansiedade , Depressão/tratamento farmacológico , Depressão/metabolismo , Transtorno Depressivo Maior/tratamento farmacológico , Hipocampo/metabolismo , Camundongos , Neurônios/metabolismo , RNA Mensageiro/metabolismo , Estresse Psicológico/metabolismo
11.
Comput Intell Neurosci ; 2022: 3838282, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35634073

RESUMO

The study intends to solve the problems of complex product circulation caused by information asymmetry and the untimely communication of production and sales information in the process of product sales to reduce the cost in the process of product circulation. Based on blockchain technology, the data integrity of the dual-channel supply chain is studied. First, the data of the supply chain conduct coordinated management to achieve the integrity of the supply chain data. Then, under the background that both retailers and suppliers are risk-neutral individuals, the benchmark model of the dual-channel supply chain is constructed, and the online and offline sales prices of products under different decision-making modes are analyzed. Finally, taking fresh agricultural products as an example, the sales strategies of the online and offline sales channels of fresh agricultural products are studied, and a dual-channel supply chain model is constructed. The profit of each member in the supply chain system under this model is obtained by the inverse method. The simulation results demonstrate that the retailer's revenue and the total revenue of the system increase obviously with the growth of the price discount coefficient after the price discount strategy is applied. When the compensation cost is between 1,000 and 3,000, the profit of retailers in the supply chain system is improved by using the price coordination mechanism, while the profit of suppliers decreases to some extent. When the value of compensation cost is 7,000-9,000, the application of the price coordination mechanism increases the profit of suppliers in the supply chain system, while the profit of retailers declines to a certain extent. The research content reported here effectively alleviates the profit conflict and the double marginal effect between the two channels and enriches the theoretical system knowledge of the coordination of the two channels' supply chain of agricultural products.


Assuntos
Blockchain , Comércio , Humanos , Tecnologia
12.
Front Psychol ; 13: 715188, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572310

RESUMO

Occupational stigma is pervasive, but there is a lack of understanding about how it impacts the behaviors of employees in relatively high-prestige occupations. We draw on the job characteristics model and social information processing theory to establish hypotheses about the effects of occupational stigma on the withdrawal behavior of employees in a relatively high-prestige occupation (preschool teacher). We suggest that perceptions of skill variety and task significance among high-prestige employees may be negatively influenced due to occupational stigma perception. In addition, occupational stigma conveys information to employees that the work they do is not appreciated by beneficiaries. For those reasons, making it difficult for them to perceive the meaningfulness of their work. This lack of meaningful experience is in turn positively associated with employees' withdrawal behavior. Furthermore, we propose that these indirect effects are moderated by perceived job embeddedness of employees. Based on data collected at two time points from 466 preschool teachers in China, we find that occupational stigma is positively related to employees' withdrawal behavior through meaningfulness. In addition, the negative relationship between perceived occupational stigma and experienced meaningfulness is stronger for employees with high job embeddedness than for employees with low job embeddedness.

13.
Nat Commun ; 13(1): 2713, 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35581266

RESUMO

Voltage-gated sodium (NaV) channels initiate action potentials. Fast inactivation of NaV channels, mediated by an Ile-Phe-Met motif, is crucial for preventing hyperexcitability and regulating firing frequency. Here we present cryo-electron microscopy structure of NaVEh from the coccolithophore Emiliania huxleyi, which reveals an unexpected molecular gating mechanism for NaV channel fast inactivation independent of the Ile-Phe-Met motif. An N-terminal helix of NaVEh plugs into the open activation gate and blocks it. The binding pose of the helix is stabilized by multiple electrostatic interactions. Deletion of the helix or mutations blocking the electrostatic interactions completely abolished the fast inactivation. These strong interactions enable rapid inactivation, but also delay recovery from fast inactivation, which is ~160-fold slower than human NaV channels. Together, our results provide mechanistic insights into fast inactivation of NaVEh that fundamentally differs from the conventional local allosteric inhibition, revealing both surprising structural diversity and functional conservation of ion channel inactivation.


Assuntos
Eucariotos , Canais de Sódio Disparados por Voltagem , Potenciais de Ação , Microscopia Crioeletrônica , Eucariotos/metabolismo , Humanos , Sódio/metabolismo , Canais de Sódio Disparados por Voltagem/genética
15.
Front Endocrinol (Lausanne) ; 13: 775278, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35528004

RESUMO

Objective: This study aims to identify reliable prognostic biomarkers for differentiated thyroid cancer (DTC) based on glycolysis-related genes (GRGs), and to construct a glycolysis-related gene model for predicting the prognosis of DTC patients. Methods: We retrospectively analyzed the transcriptomic profiles and clinical parameters of 838 thyroid cancer patients from 6 public datasets. Single factor Cox proportional risk regression analysis and Least Absolute Shrinkage and Selection Operator (LASSO) were applied to screen genes related to prognosis based on 2528 GRGs. Then, an optimal prognostic model was developed as well as evaluated by Kaplan-Meier and ROC curves. In addition, the underlying molecular mechanisms in different risk subgroups were also explored via The Cancer Genome Atlas (TCGA) Pan-Cancer study. Results: The glycolysis risk score (GRS) outperformed conventional clinicopathological features for recurrence-free survival prediction. The GRS model identified four candidate genes (ADM, MKI67, CD44 and TYMS), and an accurate predictive model of relapse in DTC patients was established that was highly correlated with prognosis (AUC of 0.767). In vitro assays revealed that high expression of those genes increased DTC cancer cell viability and invasion. Functional enrichment analysis indicated that these signature GRGs are involved in remodelling the tumour microenvironment, which has been demonstrated in pan-cancers. Finally, we generated an integrated decision tree and nomogram based on the GRS model and clinicopathological features to optimize risk stratification (AUC of the composite model was 0.815). Conclusions: The GRG signature-based predictive model may help clinicians provide a prognosis for DTC patients with a high risk of recurrence after surgery and provide further personalized treatment to decrease the chance of relapse.


Assuntos
Adenocarcinoma , Neoplasias da Glândula Tireoide , Biomarcadores Tumorais/genética , Glicólise/genética , Humanos , Recidiva Local de Neoplasia/genética , Prognóstico , Estudos Retrospectivos , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/patologia , Microambiente Tumoral
16.
Molecules ; 27(9)2022 Apr 24.
Artigo em Inglês | MEDLINE | ID: mdl-35566095

RESUMO

As compared to China's overall oil reserves, the reserve share of offshore oilfields is rather significant. However, offshore oilfield circumstances for enhanced oil recovery (EOR) include not just severe temperatures and salinity, but also restricted space on offshore platforms. This harsh oil production environment requires polymers with relatively strong salt resistance, solubility, thickening ability, rapid, superior injection capabilities, and anti-shearing ability. As a result, research into polymers with high viscosity and quick solubility is recognized as critical to meeting the criteria of polymer flooding in offshore oil reservoirs. For the above purposes, a novel hydrophobically associating polymer (HAP) was prepared to be used for polymer flooding of Bohai offshore oilfields. The synthetic procedure was free radical polymerization in aqueous solutions starting at 0 °C, using acrylamide (AM), acrylic acid (AA), 2-acrylamido-2-methylpropane sulfonic acid (AMPS), and poly(ethylene glycol) octadecyl methacrylate (POM) as comonomers. It was discovered that under ideal conditions, the molecular weight of HAP exceeds 2.1 × 107 g⋅mol-1. In a simulated reservoir environment, HAP has substantially greater solubility, thickening property, and salt resistance than conventional polyacrylamide (HPAM), with equivalent molecular weight. Finally, the injectivity and propagation of the two polymers in porous media were investigated. Compared with HPAM, which has a similar molecular weight, HAP solution with the concentration of 0.175% had a much better oil displacement effect in the porous medium, which can enhance oil recovery by 8.8%. These discoveries have the potential to pave the way for chemical EOR in offshore oilfields.


Assuntos
Petróleo , Polímeros , Campos de Petróleo e Gás , Polimerização , Polímeros/química , Água do Mar
18.
J Fungi (Basel) ; 8(5)2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35628740

RESUMO

Cordyceps chanhua is an important cordycipitoid mushroom widely used in Asia and beyond. Beauvericin (BEA), one of the bioactive compounds of C. chanhua, has attracted much attention because of its medicinal value and food safety risk. In order to clear up the relationship between oxidative stress and BEA synthesis, we investigated the impact of H2O2-induced oxidative stress on the secondary metabolism of C. chanhua using untargeted metabolomics and a transcript profiling approach. Metabolic profiling of C. chanhua mycelia found that in total, 73 differential metabolites were identified, including organic acids, phospholipids, and non-ribosomal peptides (NRPs), especially the content of BEA, increasing 13-fold under oxidative stress treatment. Combining transcriptomic and metabolomic analyses, we found that the genes and metabolites associated with the NRP metabolism, especially the BEA biosynthesis, were highly significantly enriched under H2O2-induced stress, which indicated that the BEA metabolism might be positive in the resistance of C. chanhua to oxidative stress. These results not only aid in better understanding of the resistance mechanisms of C. chanhua against oxidative stress but also might be helpful for molecular breeding of C. chanhua with low BEA content.

19.
Am J Health Behav ; 46(2): 197-208, 2022 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-35501965

RESUMO

Objectives: In this paper, we explore the relationship between the psychological capital, sport anxiety, and sport performance of collegiate judo athletes. Methods: The research object is the collegiate first-division judo athletes in Taiwan. Convenience sampling was used to conduct an online survey. A total of 106 questionnaires were issued, 102 valid questionnaires were returned, and the effective response rate was 96.23%. The data were analyzed by using confirmatory factor analysis (CFA) and structural equation modeling (SEM). Results: The psychological capital of collegiate judo athletes has a significant positive impact on sport performance; moreover, the sport anxiety of collegiate judo athletes has a significant negative impact on psychological capital. Through psychological capital, sport performance can be improved. Therefore, psychological capital has an intermediary effect. Conclusion: This study shows the higher the level of mental capital of collegiate judo athletes, the better the positive impact on sport performance; in addition, the higher the sport anxiety, the lower the psychological capital. However, sport anxiety can improve sport performance through psychological capital; therefore, we recommend that the training plan of collegiate judo athletes focus on strengthening the athletes' mental skills to improve their sport performance.


Assuntos
Artes Marciais , Ansiedade , Transtornos de Ansiedade , Atletas/psicologia , Humanos , Artes Marciais/fisiologia , Inquéritos e Questionários
20.
Proc Natl Acad Sci U S A ; 119(15): e2117529119, 2022 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35377787

RESUMO

SignificanceThe onset time of plate tectonics is highly debated in the Earth sciences. A key indicator of modern-style plate tectonics, with deep subduction of oceanic plates, is the presence of eclogite (oceanic crust metamorphosed at high-pressure and low-temperature) in orogenic belts. Since no orogenic eclogites older than 2.1 billion y are currently documented, many scientists argue that modern plate tectonics started only 2.1 billion y ago (Ga). We document an Archean orogenic eclogite, providing robust evidence that subduction of oceanic crust reached to at least 65 to 70 km in depth at circa 2.5 Ga. This extends the known age of subduction-related eclogite-facies metamorphism back 400 My, showing that modern-style plate tectonics operated by the close of the Archean.

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