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1.
Zhongguo Zhong Yao Za Zhi ; 45(2): 451-456, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-32237331

RESUMO

To investigate the efficacy of Huangqin Qingre Chubi Capsules(HQC) in patients with ankylosing spondylitis(AS) and its effect on oxidative stress, and to explore its possible mechanism. Fifty-eight cases of AS patients were randomly divided into HQC group and salazosulfapyridine(SASP) group. Another 30 healthy people were employed as a control group. Superoxide dismutase(SOD), total antioxidant capacity(TAOC), malondialdehyde(MDA), lipid peroxidatio(LPO), interleukin-1ß(IL-1ß), IL-10, IL-4, and tumor necrosis factor-α(TNF-α) were detected by ELISA. The mRNA expression levels of AMP-activated protein kinase(AMPK-α), forkhead box O3a(FOXO3a), manganese superoxide dismutase(MnSOD), and peroxisome proliferator-activated receptor gamma(PPARγ) were detected by Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR). The protein expression levels of AMPK-α, FOXO3a, p-FOXO3a, MnSOD, and PPARγ were detected by Western blot. A questionnaire was used to evaluate the disease activity score and observe the clinical efficacy of HQC in AS patients. The levels of MDA, LPO, TNF-α, and IL-1ß were significantly increased in the peripheral blood of AS patients, and SOD, TAOC, IL-4, IL-10 levels were significantly decreased. After HQC treatment, scores of disease active indexes were all decreased, and its clinical efficacy was significantly higher than that in SASP group. After HQC treatment, TAOC, SOD, IL-4, IL-10 were increased and MDA, LPO, TNF-α, IL-1ß were decreased; mRNA levels of AMPK-α, FOXO3a, MnSOD, PPARγ and protein levels of AMPK-α, FOXO3a, p-FOXO3a, MnSOD, PPARγ were increased(P<0.01 or P<0.05). HQC can effectively improve the clinical symptoms and oxidative stress of AS patients, and its mechanism may be related to activating PPARγ and up-regulating AMPK/FOXO3a signal pathway.

2.
Biosci Rep ; 2020 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-32191279

RESUMO

Objective: Circular RNAs (circRNAs) are a significant class of molecules involved in a wide range of diverse biological functions that are abnormally expressed in many types of diseases. This study aimed to determine the circRNAs specifically expressed in peripheral blood mononuclear cells (PBMCs) from rheumatoid arthritis (RA) patients to identify their possible molecular mechanisms. METHODS: To identify the circRNAs specifically expressed in RA, we started by sequencing the of PBMCs circRNA and microRNAs (miRNAs) from a RA group (n=3) and a control group (n=3). We constructed a network of differentially expressed circRNAs and miRNAs. Then, we selected differentially expressed circRNAs in PBMCs from 10 RA patients relative to 10 age- and sex-matched controls using real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR). Spearman's correlation test was used to evaluate the correlation of circRNAs with biochemical measurements. RESULTS: A total of 165 circRNAs and 63 miRNAs were differently expressed between RA patients and healthy people according to RNA-seq, including 109 circRNAs that were significantly upregulated and 56 circRNAs that were downregulated among the RA patients. RT-qPCR validation demonstrated that the expression levels of hsa_circ_0001200, hsa_circ_0001566, hsa_circ_0003972, and hsa_circ_0008360 were consistent with the results from the sequencing analysis. Then, we found that there were significant correlations between the circRNAs and disease severity. CONCLUSION: Generally, these results suggest that expression of hsa_circ_0001200, hsa_circ_0001566, hsa_circ_0003972, and hsa_circ_0008360 in PBMCs from RA patients may serve as potential biomarkers for the diagnosis of RA, and these circRNAs may influence the occurrence and development of RA.

3.
Int J Phytoremediation ; : 1-11, 2020 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-32122163

RESUMO

A laboratory experiment was performed to evaluate the potential role of H2S on cadmium (Cd) toxicity in Phlox paniculata L. Seeds pretreated with 0.3, 0.6, 0.9, and 1.2 mM NaHS as a donor of H2S for 24 h and subsequently exposed to 100, 200, and 300 µM CdCl2 for 26 days had significantly higher germination rate compared with Cd alone. Meanwhile, 2-year-old seedlings sprayed with 0.3, 0.6, and 0.9 µM NaHS were grown in soil with 0.3, 0.6, and 1.2 mg/kg CdCl2, respectively. We observed that H2S decreased Cd accumulation in leaves and elevated Cd concentration in roots. Cd toxicity in seedlings resulted in a substantial increase in Cd-induced overproduction of malondialdehyde (MDA), Cd accumulation, and electrolyte leakage. Meanwhile, addition of NaHS increased photosynthetic performance compared with Cd alone. Exogenous H2S significantly elevated biomass, improved antioxidant enzyme activities, and reduced ABA content compared with Cd alone. H2S also plays an important role in the ABA signaling pathway during stress. Notably, NaHS promoted Cd uptake by Phlox paniculate L. from soil. The prediction model of H2S for increasing plant resistance and reducing soil Cd pollution was established by factor analysis method based on comprehensive evaluation of plant stress physiology.

4.
Int J Neurosci ; : 1-16, 2020 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-32186218

RESUMO

Dental pulp stem cells (DPSCs) were widely used as seed cells in the field of tissue engineering and regenerative medicine, including spinal cord injury (SCI) repair and other neuronal degenerative diseases, due to their easy isolation, multiple differentiation potential, low immunogenicity and low rates of rejection during transplantation. Various studies have shown that bFGF can enhance peripheral nerve regeneration after injury, and phospho-ERK (p-ERK) activation as a major mediator may be involved in this process. Previous studies also have proved that a suitable biomaterial scaffold can carry and transport the therapeutic cells effectively to the recipient area. It has showed in our earlier experiments that 3D porous chitosan scaffolds exhibited a suitable circumstance for survival and neural differentiation of DPSCs in vitro. In current study, DPSCs were cultured in chitosan scaffolds and treated with neural differentiation medium for 7 days. The neural genes and protein markers were analyzed by western blot and immunofluorescence. Meanwhile, the relevant signaling pathway involved in this process was also tested. Our study revealed that the viability of DPSCs was not influenced by co-culture with the chitosan scaffolds as well as bFGF. Compared with the control and DPSC/chitosan-scaffold groups, the levels of GFAP, S100ß and ß-tubulin III significantly increased in the DPSC/chitosan-scaffold + bFGF group. Thus, chitosan scaffolds were non-cytotoxic to the survival of DPSCs, and chitosan scaffolds combined with bFGF facilitated the neural differentiation of DPSCs. The transplantation of DPSCs/chitosan-scaffold + bFGF might be a secure and effective method of treating SCI and other neuronal diseases.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32207603

RESUMO

The application of high-energy Mg/S batteries was obstructed by the insufficiency of low-cost and non-nucleophilic electrolyte. In this work, a non-nucleophilic electrolyte was prepared by facilely dissolving Mg(CF3SO3)2, MgCl2, and AlCl3 in 1,2-dimethoxyethane (DME). The equilibrium species of the MTB electrolyte mainly comprise [Mg2(µ-Cl)2(DME)4]2+ and [(CF3SO3)AlCl3]-, which are generated by dehalodimerization reaction and Lewis acid-base reaction, respectively. The electrolyte exhibits a highly efficient reversible Mg deposition/dissolution, low overpotential of around 250 mV, and good oxidative stability up to 3.5 V after conditioning. The conditioning process that the active [Mg2(µ-Cl)2(DME)4]2+ species transforms into [Mg3(µ3-Cl)(µ2-Cl)2(DME)7]3+ was demonstrated, owing to the irreversible deposition of Al3+ on Mg foil. More importantly, this electrolyte exhibited good compatibility and kinetics for reversible Mg/MgSx redox, resulting in a high specific capacity of 866 mAh g-1 at 200 mA g-1 and high power density of 550 W kg-1. This work offers a new direction for low-cost, non-nucleophilic electrolyte and paves the way to explore high-power Mg/S batteries.

6.
Med Sci Monit ; 26: e921182, 2020 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-32161254

RESUMO

BACKGROUND The 2018 Global Initiative for Chronic Obstructive Lung Disease Report reveals that the blood eosinophil count could forecast the risk of flare-ups. This study explored the correlations of blood eosinophils with fractional exhaled nitric oxide (FeNO) and pulmonary function parameters in acute exacerbation of chronic obstructive pulmonary disease (AECOPD). MATERIAL AND METHODS The data of patients with AECOPD at our hospital admitted between July 2018 and June 2019 were retrospectively analyzed. All patients were stratified into an eosinophilic group (≥2%) or a noneosinophilic group (<2%) based on the peripheral eosinophil count per centum. Cross-sectional analysis was performed to compare clinical characteristics, percentage of eosinophils, FeNO, and pulmonary function between the 2 groups. RESULTS After applying the inclusion/exclusion criteria, 247 patients were included. FeNO values were higher in eosinophilic group (n=97) than in noneosinophilic group (n=150) (P=0.005). The forced expiratory volume in 1 second% predicted (FEV1% predicted), FEV1, and forced vital capacity (FVC) were higher in the eosinophilic group than in the noneosinophilic group (P=0.043; P=0.040; and P=0.011, respectively). Blood eosinophilia showed positive correlations with FeNO (P=0.004) and spirometry variables (FEV1 [% predicted], P=0.003; FEV1, P<0.001; and FVC, P<0.001). An FeNO level of 22.5 ppb was the best cutoff value to predict blood eosinophilia (P=0.000). CONCLUSIONS Blood eosinophil count is a likely biomarker that can predict positive relationship with FeNO values and pulmonary function parameters.

7.
Drug Des Devel Ther ; 14: 417-428, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32099326

RESUMO

Background: Baicalin, a natural product isolated from Scutellaria radix, has been reported to exert anti-oxidant and anti-apoptotic effects on skin, but the underlying mechanism remains poorly understood. This study aimed to investigate the possible mechanism of anti-UVB effect of baicalin in human skin fibroblasts. Methods: Cell proliferation was estimated by CCK-8 Kit. Apoptotic incidence was detected by flow cytometry with Annexin V-PE/PI apoptosis detection kit. Autophagy was determined by the evaluation of fluorescent LC3 puncta and Western blotting. Cell signalling was analysed by Western blotting. Results: Baicalin exerted cytoprotective effects in UVB-induced HSFs. Moreover, baicalin increased autophagy and suppressed UVB-induced apoptosis of HSFs. Pretreatment with 3-MA, an autophagy inhibitor, attenuated baicalin-induced HSFs autophagy and promoted apoptosis. Baicalin activated AMPK, which leads to suppression of basal mTOR activity in cultured HSFs. Administration of compound C, an AMPK inhibitor, abrogated AMPK phosphorylation and increased mTOR phosphorylation and apoptosis compared with baicalin alone. Conclusion: Taken together, these results indicate the important role of mTOR inhibition in UVB protection by baicalin and provide a new target and strategy for better prevention of UV-induced skin disorders.

8.
J Phys Chem Lett ; : 2053-2061, 2020 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-32105076

RESUMO

Highly efficient blue-emitting three-dimensional (3D) lead-free halide perovskites with excellent stability have attracted worldwide attention. Herein, a doping route was adopted to incorporate Sb3+ ions into the Cs2NaInCl6 for decorating the electronic band structure. Due to the moderate electron-phonon coupling, the Sb3+-doped Cs2NaInCl6 double perovskites showed a narrow and relatively unusual blue emission of self-trapped excitons (STEs). Density functional theory (DFT) calculation indicated that the doped Sb3+ ions could break the parity-forbidden transition rule and modulate the density of state (DOS) population effectively to boost the PLQY of STEs drastically. The optimized Sb3+:Cs2NaInCl6 exhibited a PLQY of up to 75.89% and excellent stability under the consecutive illumination of 365 nm UV light for 1000 h. This kind of highly efficient lead-free Sb3+-doped Cs2NaInCl6 double perovskites may overcome the bottlenecks of severe toxicity and insufficient stability and therefore have an extensive application in the scarce blue photonic and optoelectronic fields.

9.
Biomed Pharmacother ; 125: 109767, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32058210

RESUMO

Diabetes mellitus (DM) is a major world health problem and one of the most studied diseases, which are highly prevalent in the whole world, it is frequently associated with severe clinical complications, such as diabetic cardiomyopathy, nephropathy, retinopathy, neuropathy etc. Scientific research is continuously casting about for new monomer molecules from Chinese herbal medicine that could be invoked as candidate drugs for fighting against diabetes and its complications. Resveratrol (RES), a polyphenol phytoalexin, possesses diverse biochemical and physiological actions, including antiplatelet, estrogenic, and anti-inflammatory properties. It is recently gaining scientific interest for RES in controlling blood sugar and fighting against diabetes and its complications properties in various types of diabetic models. These beneficial effects seem to be due to the multiple actions of RES on cellular functions, which make RES become a promising molecule for the treatment of diabetes and diabetic complications. Here, we review the mechanism of action and potential therapeutic use of RES in prevention and mitigation of these diseases in recent ten years to provide a reference for further research and development of RES.

10.
Biochem Biophys Res Commun ; 524(3): 649-655, 2020 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-32033746

RESUMO

RATIONALE: Spasmolytic polypeptide-expressing metaplasia (SPEM) is an important risk factor for the occurrence of gastric cancer. It may be driven by a chronic inflammatory environment in which macrophage is involved. Studies have shown that intestinal metaplasia may originate from SPEM, and bile acid-induced chronic inflammation plays an important role in the process of intestinal metaplasia. However, whether bile acids are involved in the development of SPEM and the specific mechanism are unclear. Meanwhile, macrophages are known to be involved in inflammation regulation by releasing various factors, including exosomes. In this study, we hypothesized that the exosomes released from macrophages stimulated by deoxycholic acid participated in the development of SPME. METHODS: In vivo, mice were gavaged with deoxycholic acid for 4 weeks, and gastric tissues were harvested. In vitro, deoxycholic acid-induced macrophage-derived exosomes were isolated by ultracentrifugation and cocultured with the gastric organoids of mice. Immunofluorescence staining and quantitative real-time PCR were used to analyze markers of macrophages and SPEM. RESULTS: In vivo, after 4 weeks of deoxycholic acid intragastric administration, macrophage markers (F4/80) and SPEM markers (TFF2 and GSII lectin) were increased in from treated mice compared with those from normal control mice. In vitro, macrophage-derived exosomes labeled with PKH67 were internalized by gastric organoids. Deoxycholic acid-induced macrophage-derived exosomes increased the expression of SPEM markers (TFF2 and GSII lectin) in gastric organoids compared to exosomes derived from macrophages without deoxycholic acid stimulation. CONCLUSION: Macrophage-derived exosomes may be a novel mechanism by which deoxycholic acid promotes SPEM.

11.
J Trop Pediatr ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32040187

RESUMO

Previous studies have shown that the CareStart™ G6PD Deficiency rapid diagnostic test has high diagnostic accuracy on G6PD deficiency in Africa and Thailand, but not in China. As there are regional differences of G6PD genotype distribution, we are attending to verify the effectiveness of the kit in Chinese population. The study cohort included 247 newborns admitted to our hospital for jaundice. The quantitative detection of G6PD enzyme activity and G6PD gene mutations analysis was used to classify the status of G6PD deficiency. The performance of CareStart™ assays was verified by calculating the sensitivity, specificity and area under the receiver operating characteristic curve (AUC) based on the corrected G6PD deficiency status. In male newborns, the sensitivity of the CareStart™ assay was 98.9%, the specificity was 94.2% and the AUC was 0.97. In female newborns, the sensitivity was 58.5% when the cutoff value of residual enzyme activity was 100%; however, the sensitivity was 100% when the cutoff value was 60%. Therefore, the CareStart™ test can effectively screen G6PD deficiency in male newborns and female infants with less than 60% residual enzyme activity, female infants with residual enzyme activities of 60-100% are more likely to be missed diagnosed among Chinese newborns.

12.
Acta Ophthalmol ; 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32043838

RESUMO

PURPOSE: To evaluate the influence of maternal gestational hypertension (GH) on retinal thickness of 5-6-year-old children, including macular thickness, ganglion cell-inner plexiform layer (GC-IPL) thickness and peripapillary retinal nerve fibre layer (RNFL) thickness. METHODS: As part of Nanjing Eye Study, comprehensive ocular examinations were conducted in children aged 61-72 months, including noncycloplegic refraction, ocular biometric parameters and retinal parameters. Retinal thickness was measured by Spectral Domain-Optical Coherence Tomography. Data on pregnancy and birth history were obtained from a detailed questionnaire completed by parents. RESULTS: Among 1062 children [mean age (standard deviation): 66.9 (3.4) months] with complete from eye examination and questionnaire, 30 (2.8%) children were born with maternal GH. In generalized linear models (adjusted for sex, age, spherical equivalent, axial length, body mass index, birth weight and premature history), children born with maternal GH had thinner average RNFL thickness (100.5 versus 104.4 µm, p = 0.035), superior RNFL thickness (123.7 versus 132.0 µm, p = 0.007), superior GC-IPL thickness (83.7 versus 86.4 µm, p = 0.005), superior-nasal GC-IPL thickness (86.3 versus 88.4 µm, p = 0.029) and superior outer macular thickness (278.0 versus 283.0 µm, p = 0.034) than children born with normal pregnancy. CONCLUSION: Children exposed to maternal GH tended to have thinner macular, RNFL and GC-IPL thickness. These findings suggest that maternal GH may affect the development of retina in children thus hinders the development of the offspring's nervous system.

13.
J Hazard Mater ; 387: 122010, 2020 04 05.
Artigo em Inglês | MEDLINE | ID: mdl-31927353

RESUMO

This study developed a novel calcium-based magnetic biochar by pyrolysing rice straw mixed with calcium carbonate and iron oxide for stabilization of contamination of multiple metals. A 160-day incubation study was conducted to investigate its performance in stabilization of cadmium and arsenic co-contamination in soil. Both biochar and Ca-MBC treatments increased soil pH, decreased the bioavailability of cadmium. Ca-MBC decreased but biochar enhanced the bioavailability of arsenic. The BCR (European Community Bureau of Reference) sequential extraction confirmed Ca-MBC facilitated the transformation of the unstable fraction of arsenic to stable fractions. The stabilization mechanisms were explored through synchrotron-based micro X-ray fluorescence and X-ray absorption near edge structure. The results show that Ca-MBC remediated the dual contamination of arsenic and cadmium through (1) elevated pH and cation exchange capacity (for Cd); (2) the formation of bi-dentate chelate and ternary surface complexes on the surface of iron oxide; (3) enhanced adsorption ability of porous biochar. In addition, Ca-MBC increased the abundance and diversity of bacterial community, and modified the relative abundances of bacterial taxa, leading to a shift of the composition. These new insights provide valuable information for stabilization of co-contamination of arsenic and cadmium in soil using the potential material Ca-MBC.

14.
Pancreatology ; 20(2): 265-277, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31956070

RESUMO

BACKGROUND: Successful clinical evaluation of human tumors relies on proper handling of tissue samples to maximally preserve the cellular and metabolic states in vivo. Pancreatic samples are particularly sensitive to sample mishandling due to the abundance of digestive enzymes. We study how the duration of ischemia, in vivo and ex vivo, both of which are unavoidable lagging periods following surgical dissection, significantly impact the utility of pancreatic samples. METHODS: We systematically characterize a wide range of tissue integrity features, including histological patterns, cellular structures, DNA/RNA quality and activity of major signaling pathways in normal pancreases and pancreatic ductal adenocarcinoma (PDAC) tumor tissues from 41 patients with different ischemia. RESULTS: We reveal that tissues experiencing longer periods of ischemia exhibit significant deterioration and could potentially mislead disease diagnosis and preclinical research. Based on these analyses, we propose an optimal procedure that balances better clinical practice and high tissue sample quality. CONCLUSIONS: Our work provides a guideline for pancreatic sample handling and could have wide implications in clinical diagnosis and translational research.

15.
J Sep Sci ; 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-31981302

RESUMO

The present study aimed to develop a strategy involving quantitative analysis of multi-components by single marker in combination with HPLC fingerprint qualitative analysis for performing the quality control of Aurantii Fructus. The content of 12 components (eriocitrin, neoeriocitrin, narirutin, naringin, hesperidin, neohesperidin, meranzin, poncirin, naringenin, nobiletin, tangeretin, and auraptene) in samples was determined using reliable relative correction factors that were obtained using naringin as an internal reference standard. The new method demonstrated good applicability, and no significant differences were observed between the external standard method and the new method as determined by calculating standard method difference. Qualitative evaluation of samples was conducted using similarity analysis, hierarchical cluster analysis, and quality fluctuation analysis. Chromatographic fingerprint data were divided into three groups by similarity and hierarchical cluster analyses, and seven components may have a more significant impact on the quality of Aurantii Fructus in quality fluctuation analysis. Overall, the study suggests that the qualitative and quantitative analyses of multi-components using quantitative analysis of multi-components by single marker combined with chromatographic fingerprinting, can be considered good quality criteria for performing quality control and providing technical support for the further pharmacological and pharmaceutical research of Aurantii Fructus. This article is protected by copyright. All rights reserved.

16.
Ultrason Sonochem ; 63: 104950, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31952007

RESUMO

This study presents a state-of-the-art overview on the application of ultrasound technology in the drying of food products, including the ultrasound pre-treatment and ultrasound assisted drying. The effect of main parameters and ultrasound technology on the drying kinetics and food quality were discussed. Inconsistencies were pointed out and analyzed in detail. Results showed that for ultrasound pre-treatment, the food products may lose or gain water and increase of ultrasonic parameters (sonication time, amplitude and ultrasound power) promoted the water loss or water gain. When ultrasound technology was applied prior to drying, an increase in drying kinetics was always observed, though some different results were also presented. For ultrasound assisted drying, the ultrasound power always gave a positive effect on the drying process, however, the magnitude of ultrasound improvement was largely dependent on the process variables, such as air velocity, air temperature, microwave power and vacuum pressure, etc. The application of ultrasound technology will somehow affect the food quality, including the physical and chemical ones. Generally, the ultrasound application can decrease the water activity, improve the product color and reduce the nutrient loss.

17.
J Hazard Mater ; 384: 121470, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31648892

RESUMO

Steam-activated biochar (SBC) was prepared and showed excellent performance for synergistic removal of Cu2+ and tetracycline (TC). The adsorption capacity of SBC and mutual effect of TC and Cu2+ were investigated via single and binary system and the adsorption isotherm. The adsorption capacity of TC was significantly enhanced when it coexisted with Cu2+. Likewise, increased amounts of Cu2+ were adsorbed in the presence of TC. The presence of NaCl exerted a negative influence on the adsorption of Cu2+, while the inhibitory effect of salinity on TC was neutralized by bridge enhancement in the binary system. Bridge enhancement and site competition were involved in the synergistic removal of TC and Cu2+. Considering the stable application in simulated and real water samples, SBC showed great potential for synergistic removal of antibiotics and heavy metals.

18.
Angew Chem Int Ed Engl ; 59(8): 3226-3234, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-31756258

RESUMO

Pathogenesis hallmarks for tuberculosis (TB) are the Mycobacterium tuberculosis (Mtb) escape from phagolysosomal destruction and limited drug delivery into infected cells. Several nanomaterials can be entrapped in lysosomes, but the development of functional nanomaterials to promote phagolysosomal Mtb clearance remains a big challenge. Here, we report on the bactericidal effects of selenium nanoparticles (Se NPs) against Mtb and further introduce a novel nanomaterial-assisted anti-TB strategy manipulating Ison@Man-Se NPs for synergistic drug-induced and phagolysosomal destruction of Mtb. Ison@Man-Se NPs preferentially entered macrophages and accumulated in lysosomes releasing Isoniazid. Surprisingly, Ison@Man-Se/Man-Se NPs further promoted the fusion of Mtb into lysosomes for synergistic lysosomal and Isoniazid destruction of Mtb. Concurrently, Ison@Man-Se/Man-Se NPs also induced autophagy sequestration of Mtb, evolving into lysosome-associated autophagosomal Mtb degradation linked to ROS-mitochondrial and PI3K/Akt/mTOR signaling pathways. This novel nanomaterial-assisted anti-TB strategy manipulating antimicrobial immunity and Mtb clearance may potentially serve in more effective therapeutics against TB and drug-resistant TB.

19.
Biomed Chromatogr ; 34(3): e4767, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31785600

RESUMO

Disorders of certain branched-chain amino acids may be associated with the occurrence and development of non-alcoholic fatty liver disease. Measurement of related branched-chain amino acid levels could provide a reference for the clinical and scientific research of the non-alcoholic fatty liver disease. An established HPLC-FLD method was used to quantify aspartic acid, glutamate, glutamine, glycine, taurine, tyrosine, 4-amino butanoic acid, tryptophan, methionine, valine, phenylalanine, isoleucine and leucine in mouse brain tissue. Brain tissue samples mixed with internal standard (3-aminobutyric acid) were processed, then derivatized with 2-O-phthaldialdehyde, and finally separated on an ODS2 column through gradient elution at a flow rate of 1.0 ml·min-1 . The excitation and emission wavelengths were set at 340 and 455 nm, respectively. The mobile phase A was 100% methanol and the mobile phase B consisted of 30 mmol·L-1 sodium acetate (pH 6.8). The injection volume was 20 µl and the single run time was 45 min. Several parameters, accuracy, precision, and stability, were verified and the results showed the established method had good sensitivity and resolution for all of the 13 compounds and internal standard in mouse brain.

20.
J Ethnopharmacol ; 248: 112317, 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-31629862

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Arnebiae Radix, a common herbal medicine in China, is often utilized to treat blood-heat syndrome and has been reported to exert an effect on the heart. AIM OF THE STUDY: The combination of acetylcholine (Ach) and CaCl2 has been widely used to induce atrial fibrillation (AF) in animals. However, whether Arnebiae Radix displays any preventive action on Ach-CaCl2 induced AF in rats remains uncertain. In our study, we attempted to investigate the protective effects of Arnebiae Radix on Ach-CaCl2 induced AF compared to amiodarone, which was employed as the positive control. MATERIALS AND METHODS: To establish the AF model, SD rats were treated with a mixture of 0.1 mL/100 g Ach-CaCl2 (60 µg/mL Ach and 10 mg/mL CaCl2) by tail vein injection for 7 days. Rats were also given a gavage of Arnebiae Radix (0.18 g/mL) one week before or concurrently with the establishment of the AF model. At the end of the experimental period, the induction, duration and timing of AF were monitored using electrocardiogram recordings. Left atrial tissues were stained to observe the level of fibrosis. Electrophysiological measurements were used to examine atrial size and function. RESULTS: In Ach-CaCl2-induced AF rats, Arnebiae Radix decreased AF induction, duration and susceptibility to AF. In addition, Arnebiae Radix significantly reduced atrial fibrosis and inhibited atrial enlargement induced by Ach-CaCl2. Moreover, there was an apparent improvement in cardiac function in the Arnebiae Radix-treated group. CONCLUSIONS: Our findings indicate that Arnebiae Radix treatment can attenuate Ach-CaCl2-induced atrial injury and serve as an effective therapeutic strategy for the treatment of AF in the future.

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