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1.
Inorg Chem ; 2020 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-32286802

RESUMO

Efficient CO2 reduction with earth-abundant photocatalysts is a highly attractive but very challenging process for chemists. Herein, we synthesized an indium-porphyrin framework, In(H2TCPP)(1-n)[Fe(TCPP)(H2O)](1-n)[DEA](1-n) (In-FenTCPP-MOF; H2TCPP = tetrakis(4-benzoic acid)porphyrine; DEA = diethylamine), with a porphyrin ring supporting the single-site iron for the high-performance visible-light-driven conversion of CO2 to CO. A high CO yield of 3469 µmol g-1 can be achieved by a 24 h photocatalytic reaction with a high CO selectivity (ca. 99.5%). This activity was much higher than that of its cobalt analogues or the Fe-free indium-based metal-organic framework (MOF). Systematic experimental and theoretical studies indicate that the porphyrin-supported iron centers in the MOF matrix serve as efficient active sites, which can accept electrons from the photoexcited MOFs in order to mediate CO2 reduction.

2.
Chem Commun (Camb) ; 56(27): 3851-3854, 2020 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-32134081

RESUMO

Five molecular complexes with different non-noble metal centers were synthesized. The Co-based complex displays the highest photocatalytic performance for CO2 to CO conversion in aqueous media. It achieves high activity (TON = 41 017 and TOF = 3.80 s-1) and selectivity (87%) for the production of CO.

3.
Med Sci Monit ; 26: e921233, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32032347

RESUMO

BACKGROUND Osteosarcoma is a common malignant tumor of musculoskeletal stromal cells. Osteosarcoma clinical behavior depends mostly on the histologic grade, the site of primary tumor, the response to chemotherapy, and the presence of pulmonary metastases. The aim of this study was to knockout SHOX CNE9/10 in U2OS osteosarcoma cells and to analyze the effects on cell growth and apoptosis. MATERIAL AND METHODS U2OS cells with CNE9 knockout and U2OS cells with CNE10 knockout were established via the CRISPR/Cas9 system. Sanger sequencing was used to detect the success of the knockdown experiment. Western blotting and quantitative polymerase chain reaction were used to detect the expression levels of short stature homeobox-containing gene (SHOX) protein and messenger RNA (mRNA) after knockdown of CNE9 and CNE10. The cell viability and apoptotic rate were detected by the Cell Counting Kit-8 method and by flow cytometry. RESULTS The Sanger sequencing results showed that the knockdown experiment was successful. The levels of SHOX mRNA and protein were significantly reduced after knocking down CNE9 and CNE10. Knockdown of CNE9 and CNE10 significantly increased the growth and inhibited the apoptosis of U2OS osteosarcoma cells. CNE9/CNE10 knockdown U2OS cells were successfully constructed. CONCLUSIONS Knockdown of CNE9 and CNE10 promoted U2OS cell growth and inhibited apoptosis by decreasing SHOX expression. This CNE9/CNE10 knockout U2OS cell model could provide a bridge for the research on SHOX and CNEs in osteosarcoma.

4.
Angew Chem Int Ed Engl ; 58(32): 10923-10927, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31162784

RESUMO

There is a demand to develop molecular catalysts promoting the hydrogen evolution reaction (HER) with a high catalytic rate and a high tolerance to various inhibitors, such as CO and O2 . Herein we report a cobalt catalyst with a penta-dentate macrocyclic ligand (1-Co), which exhibits a fast catalytic rate (TOF=2210 s-1 ) in aqueous pH 7.0 phosphate buffer solution, in which proton transfer from a dihydrogen phosphate anion (H2 PO4 - ) plays a key role in catalytic enhancement. The electrocatalyst exhibits a high tolerance to inhibitors, displaying over 90 % retention of its activity under either CO or air atmosphere. Its high tolerance to CO is concluded to arise from the kinetically labile character of undesirable CO-bound species due to the geometrical frustration posed by the ligand, which prevents an ideal trigonal bipyramid being established.

5.
Angew Chem Int Ed Engl ; 57(50): 16480-16485, 2018 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-30362217

RESUMO

The solar-driven CO2 reduction is a challenge in the field of "artificial photosynthesis", as most catalysts display low activity and selectivity for CO2 reduction in water-containing reaction systems as a result of competitive proton reduction. Herein, we report a dinuclear heterometallic complex, [CoZn(OH)L1 ](ClO4 )3 (CoZn), which shows extremely high photocatalytic activity and selectivity for CO2 reduction in water/acetonitrile solution. It achieves a selectivity of 98 % for CO2 -to-CO conversion, with TON and TOF values of 65000 and 1.8 s-1 , respectively, 4, 19, and 45-fold higher than the values of corresponding dinuclear homometallic [CoCo(OH)L1 ](ClO4 )3 (CoCo), [ZnZn(OH)L1 ](ClO4 )3 (ZnZn), and mononuclear [CoL2 (CH3 CN)](ClO4 )2 (Co), respectively, under the same conditions. The increased photocatalytic performance of CoZn is due to the enhanced dinuclear metal synergistic catalysis (DMSC) effect between ZnII and CoII , which dramatically lowers the activation barriers of both transition states of CO2 reduction.

6.
ChemSusChem ; 11(6): 1025-1031, 2018 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-29385321

RESUMO

The reduction of carbon dioxide (CO2 ) has been considered as an approach to mitigate global warming and to provide renewable carbon-based fuels. Rational design of efficient, selective, and inexpensive catalysts with low overpotentials is urgently desired. In this study, four cobalt(II) tripodal complexes are tested as catalysts for CO2 reduction to CO in a MeCN/H2 O (4:1 v/v) solution. The replacement of pyridyl groups in the ligands with less basic quinolinyl groups greatly reduces the required overpotential for CO2 -to-CO conversion down to 200-380 mV. Benefitting from the low overpotentials, a photocatalyst system for CO2 -to-CO conversion is successfully constructed, with an maximum turnover number (TON) of 10 650±750, a turnover frequency (TOF) of 1150±80 h-1 , and almost 100 % selectivity to CO. These outstanding catalytic performances are further elucidated by DFT calculations.

7.
Chemistry ; 24(18): 4503-4508, 2018 Mar 26.
Artigo em Inglês | MEDLINE | ID: mdl-29322569

RESUMO

A catalyst developed from a CuII complex of (Et4 N)[Cu(pyN2Me2 )(HCO2 )]⋅0.5 CH3 OH⋅H2 O (1⋅0.5 CH3 OH⋅H2 O; pyN2Me2 =bis(2,6-dimethylphenyl)-2,6-pyridinedicarboxamidate(2-)) shows a high activity to catalyze the reduction reaction of CO2 to CO driven by visible light in 4:1 acetonitrile/water (v:v) using [Ru(phen)3 ](PF6 )2 as photosensitizer and TEOA as sacrificial reductant, with a high TON of 9900 and a high CO selectivity of 98 %. The results of isotope labeling experiment, durability tests and energy dispersive spectroscopy reveal that 1 is robust during the photocatalytic process.

8.
Chem Commun (Camb) ; 53(67): 9324-9327, 2017 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-28782066

RESUMO

Herein we report that a Cu(ii) salt can efficiently catalyze water oxidation in a neutral borate buffer. Studies on the mechanism revealed that the borate anion serves as an oxygen donor in O-O bond formation during the process of water oxidation, assisting in enhancing the catalytic activity of Cu(ii).

9.
Angew Chem Int Ed Engl ; 56(3): 738-743, 2017 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-27958678

RESUMO

A dinuclear cobalt complex [Co2 (OH)L1 ](ClO4 )3 (1, L1 =N[(CH2 )2 NHCH2 (m-C6 H4 )CH2 NH(CH2 )2 ]3 N) displays high selectivity and efficiency for the photocatalytic reduction of CO2 to CO in CH3 CN/H2 O (v/v=4:1) under a 450 nm LED light irradiation, with a light intensity of 100 mW cm-2 . The selectivity reaches as high as 98 %, and the turnover numbers (TON) and turnover frequencies (TOF) reach as high as 16896 and 0.47 s-1 , respectively, with the calculated quantum yield of 0.04 %. Such high activity can be attributed to the synergistic catalysis effect between two CoII ions within 1, which is strongly supported by the results of control experiments and DFT calculations.

10.
ChemSusChem ; 9(5): 485-91, 2016 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-26785219

RESUMO

The reaction of N,N'-dimethyl-N,N'-bis(pyridin-2-ylmethyl)-1,2-diaminoethane ligand (L) with Ni(ClO4)2 ⋅6 H2O generated a complex of [NiL(H2O)2](ClO4)2 (1) with two cis labile sites occupied by two coordinated H2O molecules, which can homogeneously electrocatalyze water oxidation in pH 6.5 acetate (OAc(-)) buffer at room temperature. The catalytic mechanism was studied by electrochemical experiments and density functional theory calculations to elucidate the following steps: (a) one of two water molecules in 1 is exchanged by OAc(-) to generate [NiL(H2O)(OAc)](+) when dissolved in OAc(-) buffer, (b) Ni(II) is directly oxidized to Ni(IV) and OAc(-) is replaced with OH(-) to form [Ni(IV) L(OH)2 ](2+), and (c) a peroxide intermediate is formed through the intramolecular O-O coupling in the presence of OAc(-), which undergoes further oxidation to release O2.


Assuntos
Níquel/química , Água/química , Catálise , Modelos Moleculares , Oxirredução
11.
J Plast Reconstr Aesthet Surg ; 68(4): 571-9, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25752717

RESUMO

BACKGROUND: Perforator-based flaps have been explored across almost all of the lower leg except in the Achilles tendon area. This paper introduced a perforator flap sourced from this area with regard to its anatomic basis and clinical applications. METHODS: Twenty-four adult cadaver legs were dissected to investigate the perforators emerging along the lateral edge of the Achilles tendon in terms of number and location relative to the tip of the lateral malleolus, and distribution. Based on the anatomic findings, perforator flaps, based on the perforator(s) of the lateral calcaneal artery (LCA) alone or in concert with the perforator of the peroneal artery (PA), were used for reconstruction of lower-posterior heel defects in eight cases. Postoperatively, subjective assessment and Semmes-Weinstein filament test were performed to evaluate the sensibility of the sural nerve-innerved area. RESULTS: The PA ended into the anterior perforating branch and LCA at the level of 6.0 ± 1.4 cm (range 3.3-9.4 cm) above the tip of the lateral malleolus. Both PA and LCA, especially the LCA, gave rise to perforators to contribute to the integument overlying the Achilles tendon. Of eight flaps, six were based on perforator(s) of the LCA and two were on perforators of the PA and LCA. Follow-up lasted for 6-28 months (mean 13.8 months), during which total flap loss and nerve injury were not found. Functional and esthetic outcomes were good in all patients. CONCLUSION: The integument overlying the Achilles tendon gets its blood supply through the perforators of the LCA primarily and that of through the PA secondarily. The LCA perforator(s)-based and the LCA plus PA perforators-based stepladder flap is a reliable, sensate flap, and should be thought of as a valuable procedure of choice for coverage of lower-posterior heel defects in selected patients.


Assuntos
Calcanhar/cirurgia , Retalho Perfurante/irrigação sanguínea , Procedimentos Cirúrgicos Reconstrutivos/métodos , Tendão do Calcâneo , Adolescente , Adulto , Cadáver , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
12.
Int J Radiat Oncol Biol Phys ; 91(1): 206-12, 2015 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-25442332

RESUMO

PURPOSE: To more accurately define clinical target volume for cervical cancer radiation treatment planning by evaluating tumor microscopic extension toward the uterus body (METU) in International Federation of Gynecology and Obstetrics stage Ib-IIa squamous cell carcinoma of the cervix (SCCC). PATIENTS AND METHODS: In this multicenter study, surgical resection specimens from 318 cases of stage Ib-IIa SCCC that underwent radical hysterectomy were included. Patients who had undergone preoperative chemotherapy, radiation, or both were excluded from this study. Microscopic extension of primary tumor toward the uterus body was measured. The association between other pathologic factors and METU was analyzed. RESULTS: Microscopic extension toward the uterus body was not common, with only 12.3% of patients (39 of 318) demonstrating METU. The mean (±SD) distance of METU was 0.32 ± 1.079 mm (range, 0-10 mm). Lymphovascular space invasion was associated with METU distance and occurrence rate. A margin of 5 mm added to gross tumor would adequately cover 99.4% and 99% of the METU in the whole group and in patients with lymphovascular space invasion, respectively. CONCLUSION: According to our analysis of 318 SCCC specimens for METU, using a 5-mm gross tumor volume to clinical target volume margin in the direction of the uterus should be adequate for International Federation of Gynecology and Obstetrics stage Ib-IIa SCCC. Considering the discrepancy between imaging and pathologic methods in determining gross tumor volume extent, we recommend a safer 10-mm margin in the uterine direction as the standard for clinical practice when using MRI for contouring tumor volume.


Assuntos
Carcinoma de Células Escamosas/patologia , Carga Tumoral , Neoplasias do Colo do Útero/patologia , Útero/patologia , Adulto , Fatores Etários , Análise de Variância , Vasos Sanguíneos/patologia , Carcinoma de Células Escamosas/radioterapia , Carcinoma de Células Escamosas/cirurgia , Colo do Útero/patologia , Feminino , Humanos , Histerectomia/métodos , Linfonodos/patologia , Imagem por Ressonância Magnética/métodos , Pessoa de Meia-Idade , Invasividade Neoplásica , Estadiamento de Neoplasias , Neoplasias do Colo do Útero/radioterapia , Neoplasias do Colo do Útero/cirurgia
13.
Biochem Biophys Res Commun ; 441(1): 191-5, 2013 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-24140062

RESUMO

Cu/Zn superoxide dismutase (SOD1) is a key antioxidant enzyme. Deficiency of SOD1 is associated with various human diseases, including cancer. Here, we report that SOD1 is succinylated and that succinylation decreases its activity. SIRT5 binds to, desuccinylates and activates SOD1. SOD1-mediated ROS reduction is increased when SIRT5 is co-expressed. Furthermore, mutation of the SOD1 succinylation site inhibits the growth of lung tumor cells. These results reveal a novel post-translational regulation of SOD1 by means of succinylation and SIRT5-dependent desuccinylation, which is important for the growth of lung tumor cells.


Assuntos
Espécies Reativas de Oxigênio/metabolismo , Sirtuínas/metabolismo , Succinatos/metabolismo , Superóxido Dismutase/metabolismo , Sequência de Aminoácidos , Biocatálise , Linhagem Celular Tumoral , Proliferação de Células , Ativação Enzimática , Humanos , Neoplasias Pulmonares/enzimologia , Neoplasias Pulmonares/patologia , Lisina/metabolismo , Dados de Sequência Molecular , Mutação/genética , Ligação Proteica , Superóxido Dismutase/química , Superóxido Dismutase-1
14.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(7): 1943-8, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-24059206

RESUMO

With comparison of the fluorescence spectroscopy and plasma-mass spectroscopy analysis results of the Meso/Cenozoic basic rocks of SE China, the authors found that the average SiO2 content of the Mesozoic basic rocks in this area is about 50%, while that of the Cenozoic basic rocks is about 43%. The former belongs to the basic group and the later to the ultrabasic group in igneous rock classification. Cenozoic basalts, accompanied with high magnesium content and low silica-alumina component, are obviously basic or ultrabasic rocks. Distinctive difference in the content of trace elements and of REE is also presented between the Mesozoic and the Cenozoic basic rocks. Distribution models of both trace elements and REE of the Mesozoic basic rocks are similar to those of the upper crust, and the models of the Cenozoic basic rocks are like those of OIB, indicating that basic rocks of the Cenozoic and OIB should originate from the mantle while that of the Mesozoic is from the bottom part of the upper crust with relationship to the evolution of the Mesozoic crustal magma layer of this area.

15.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(5): 1369-73, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-23905354

RESUMO

The results on Xilingang pluton, mainly consisting of red beds, granites containing numerous debris of red beds and granites, obtained by X-ray fluorescence spectroscopy, plasma mass spectrometry and Raman spectroscopy show: (1) Xilingang pluton from red beds, granites containing numerous debris of red beds to granites has obvious characteristics of decreasing silicon and alkali content, and rising ignition loss, dark mineral content and oxidation index; (2) Chondrite-normalized REE distribution curves and primitive mantle-normalized spider diagram for trace elements of redbed, granites containing numerous debris of red beds and granites have a good consistency, the distribution characteristics of elements are similar to Nanling transformation-type granite; (3) The value of Raman spectrogram characteristic peak of quartz crystal in Xilingang granite decreased from the center of quartz crystal, and FWHM is steady. According to the above, the authors believe that Xilingang granite formed was related to in-situ melting of red beds and underlying strata and magma consolidation. Volatile components were discharged continuously, and oxidation index decreased gradually in the melting process. In the process of diagenesis, the top of pluton tend to be an ongoing silicon and alkali increase, while TFeO and MgO continue to migrate to bottom, and crystallization environment is a relatively closed and steady system.

16.
BMC Cancer ; 13: 247, 2013 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-23688241

RESUMO

BACKGROUND: Evidence suggests that cytoglobin (Cygb) may function as a tumor suppressor gene. METHODS: We immunohistochemically evaluated the expression of Cygb, phosphatidylinositol-3 kinase (PI-3K), phosphorylated (p)-Akt, Interleukin-6 (IL-6), tumor necrosis factor-α (TNFα) and vascular endothelial growth factor (VEGF) in 88 patients with 41 high-grade gliomas and 47 low-grade gliomas. Intratumoral microvessel density (IMD) was also determined and associated with clinicopathological factors. RESULTS: Low expression of Cygb was significantly associated with the higher histological grading and tumor recurrence. A significant negative correlation emerged between Cygb expression and PI3K, p-Akt, IL-6, TNFα or VEGF expression. Cygb expression was negatively correlated with IMD. There was a positive correlation between PI3K, p-Akt, IL-6, TNFα and VEGF expression with IMD.High histologic grade, tumor recurrence, decreased Cygb expression, increased PI3K expression, increased p-Akt expression and increased VEGF expression correlated with patients' overall survival in univariate analysis. However, only histological grading and Cygb expression exhibited a relationship with survival of patients as independent prognostic factors of glioma by multivariate analysis. CONCLUSIONS: Cygb loss may contribute to tumor recurrence and a worse prognosis in gliomas. Cygb may serve as an independent predictive factor for prognosis of glioma patients.


Assuntos
Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Glioma/metabolismo , Glioma/patologia , Globinas/metabolismo , Recidiva Local de Neoplasia/metabolismo , Adolescente , Adulto , Idoso , Análise de Variância , Citoglobina , Feminino , Humanos , Interleucina-1/metabolismo , Estimativa de Kaplan-Meier , Masculino , Microvasos/patologia , Pessoa de Meia-Idade , Análise Multivariada , Gradação de Tumores , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação , Modelos de Riscos Proporcionais , Proteínas Proto-Oncogênicas c-akt/metabolismo , Estudos Retrospectivos , Transdução de Sinais , Fator de Necrose Tumoral alfa/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Adulto Jovem
17.
Cancer Invest ; 26(3): 296-305, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18317971

RESUMO

BACKGROUND & AIMS: To investigate relationships between basement membrane structure, inflammation, beta1 integrin expression, activation of ERK/MAPK signaling pathways, and cell proliferation in esophageal mucosa at various stages during the evolution of esophageal squamous cell carcinoma. METHODS: Three tissue arrays were made of 228 tissue cores from 428 surgically-resected specimens. The arrays included 26 samples of normal epithelium, 28 with hyperplasia, 18 with dysplasia, 27 with carcinoma in situ and 129 with invasive carcinoma. In addition, 21 cases of hyperplasia, 13 cases of dysplasia and 13 case of carcinoma in situ were obtained by manual microdissection of unfixed frozen tissue. Hematoxylin and eosin stained sections were used to evaluate the epithelium and inflammation. The periodic acid-Schiff stain and an immunohistochemical stain for laminin were used to examine the structure of basement membranes. The expression of beta1 integrin, p-ERK, and Ki67 were evaluated by quantitative immunohistochemistry. RT-PCR and Western blots were also used to detect expression of beta1 integrin. RESULTS: Quantitative scales were developed to classify basement membrane structure and inflammation. Basement membrane alterations correlated with the degree of epithelial change (chi2 = 501.9, p < 0.01) and with the degree of lymphocytic infiltration in the lamina propria and epithelium (chi2 = 273.4, p < 0.01). There was a significant relationship between the extent of basement membrane alteration and the expression of beta1 integrin, p-ERK, and Ki67. CONCLUSIONS: The correlations suggest that there is a direct relationship between basement membrane structure and the development of esophageal squamous cell carcinoma.


Assuntos
Membrana Basal/patologia , Carcinoma de Células Escamosas/patologia , Transformação Celular Neoplásica/patologia , Neoplasias Esofágicas/patologia , Inflamação/patologia , Membrana Basal/metabolismo , Western Blotting , Carcinoma de Células Escamosas/metabolismo , Proliferação de Células , Transformação Celular Neoplásica/metabolismo , China , Neoplasias Esofágicas/metabolismo , MAP Quinases Reguladas por Sinal Extracelular/biossíntese , Humanos , Imuno-Histoquímica , Cadeias beta de Integrinas/biossíntese , Antígeno Ki-67/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Risco , Transdução de Sinais/fisiologia , Análise Serial de Tecidos
18.
Sheng Wu Gong Cheng Xue Bao ; 23(5): 907-14, 2007 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-18051874

RESUMO

Yeast is a widely used host for recombinant protein expression. However, glycoproteins derived from yeast contain N-glycan of high mannose type and are usually hyperglycosylated. alpha-1,6-mannosyltransferases gene (och1) encodes the enzyme that initiates the first step of out-chain elongation of high mannose type N-glycan in yeast, which is different from that in human. So, a high efficient method to knockout target gene by two-step recombination was established and was used to delete och1. In the first recombinant, a plasmid with och1::ADE1 and ura3 gene was linearized in the downstream of och1 and inserted to the och1 site of P. pastoris genome, where the upstream and downstream of och1 were duplicated. In the second recombinant, the duplicated fragments of och1 were exchanged and the och1 deletion strains were selected on the plates containing 5-FOA, but no adenine. Then the och1 deletion strain was applied to express an human serum albumin (HSA) granulocyte-macrophage colony-stimulating factor (GM-CSF) chimera. Different with the hyperglycosylated HSA/GM-CSF chimera expressed in wild type P. pastoris, the chimera expressed in the och1 deletion strain, contained smaller N-glycan. The results suggested that the och1 mutant yeast may be more suitable for production of recombinant glycoproteins. And the och 1 deletion strain could be used for further re-engineering to produce complex human glycoproteins.


Assuntos
Fator Estimulador de Colônias de Granulócitos e Macrófagos/genética , Manosiltransferases/genética , Pichia/genética , Albumina Sérica/genética , Quimera , Deleção de Genes , Técnicas de Inativação de Genes , Fator Estimulador de Colônias de Granulócitos e Macrófagos/biossíntese , Pichia/enzimologia , Pichia/metabolismo , Proteínas Recombinantes de Fusão/biossíntese , Proteínas Recombinantes de Fusão/genética , Albumina Sérica/biossíntese
19.
Zhongguo Zhen Jiu ; 27(9): 685-6, 2007 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-17926624

RESUMO

OBJECTIVE: To observe the clinical effect of acupuncture for controlling the adverse response in gastroscopy. METHODS: Ninety-seven cases of gastroscopy were randomly divided into an observation group of 52 cases and a control group of 45 cases. The observation group were treated by acupuncture at Hegu (LI 4), Neiguan (PC 6) and Zusanli (ST 36), in combined with oral administration of Lidocaine; the control group were treated by simple administration of Lidocaine. Changes of the adverse response, blood pressure and heart rate, and satisfactory degrees and the willing re-examination rate were investigated in the two groups. Results In the observation group, the nausea and vomiting, salivation, restlessness, breath holding and other adverse responses in gastroscopy were significantly decreased as compared with those in the control group (P < 0.01), and the blood pressure and heart rate were more stable than in the control group, and the satisfactory degree and willing re-examination rate were higher than the control group (P < 0.01). CONCLUSION: Acupuncture can effectively control the adverse response in gastroscopy.


Assuntos
Terapia por Acupuntura , Gastroscopia , Adulto , Pressão Sanguínea , Feminino , Frequência Cardíaca , Humanos , Masculino
20.
Genomics ; 90(4): 474-81, 2007 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17689918

RESUMO

There are three major geographic regions in China known for their high incidences of esophageal cancer (EC): the Taihang Mountain range of north-central China, the Minnan area of Fujian province, and the Chaoshan plain of Guangdong province. Historically, waves of great population migrations from north-central China through coastal Fujian to the Chaoshan plain were recorded. To study the genetic relationship among the related EC high-risk populations, we analyzed mitochondrial DNA (mtDNA) haplogroups based on 30 EC patients from Chaoshan and used control samples from the high-risk populations, including 48, 73, and 89 subjects from the Taihang, Fujian, and Chaoshan areas, respectively. The principal component of all haplogroups, correlation analysis of haplogroup frequency distributions between populations, and haplogroup D network analysis showed that compared with other Chinese populations, populations in the three studied areas are genetically related. The highest haplogroup frequency shared by all studied populations was haplogroup D, with much higher frequency in the Chaoshan area EC patients. The majority of haplogroup D individuals among the Chaoshan area EC patients belonged to subhaplogroups D4a and D5a, with the total frequency of these two haplogroups significantly higher than that in the high-risk population in the same area (chi(2)=9.017, p<0.01). In conclusion, EC high-risk populations in these three areas share a similar matrilineal genetic background, and D4a and D5a might be candidate genetic markers for screening populations susceptible to EC in the Chaoshan area. Ours is the first report to show the association between mtDNA haplogroups (D4a and D5a) and esophageal cancer.


Assuntos
DNA Mitocondrial/análise , Neoplasias Esofágicas/genética , Predisposição Genética para Doença , Adulto , Estudos de Casos e Controles , China , Frequência do Gene , Genética Populacional , Geografia , Haplótipos , Humanos , Masculino , Filogenia , Fatores de Risco
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