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1.
CNS Neurosci Ther ; 2021 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-34939745

RESUMO

INTRODUCTION: Epilepsy is a serious hazard to human health. Minimally invasive surgery is an extremely effective treatment to refractory epilepsy currently if the location of epileptic foci is given. However, it is challenging to locate the epileptic foci since a multitude of patients are MRI-negative. It is well known that DKI (diffusion kurtosis imaging) can analyze the pathological changes of local tissues and other regions of epileptic foci at the molecular level. In this article, we propose a new localization way for epileptic foci based on machine-learning method with kurtosis tensor in DKI. METHODS: We recruited 59 children with hippocampus epilepsy and 70 age- and sex-matched normal controls; their T1-weighted images and DKI were collected simultaneously. Then, the hippocampus in DKI is segmented based on a mask as a local brain region, and DKE is utilized to estimate the kurtosis tensor of each subject's hippocampus. Finally, the kurtosis tensor is fed into SVM (support vector machine) to identify epilepsy. RESULTS: The classifier produced 95.24% accuracy for patient versus normal controls, which is higher than that obtained with FA (fractional anisotropy) and MK (mean kurtosis). Experimental results show that the kurtosis tensor is a kind of remarkable feature to identify epilepsy, which indicates that DKI images can act as an important biomarker for epilepsy from the view of clinical diagnosis. CONCLUSION: Although the classification task for epileptic patients and normal controls discussed in this article did not directly achieve the location of epileptic foci and only identified epilepsy on certain brain region, the epileptic foci can be located with the results of identifying results on other brain regions.

2.
Nutrients ; 13(11)2021 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-34836220

RESUMO

Grapes provide a rich source of polyphenols and fibers. This study aimed to evaluate the effect of the daily consumption of 46 g of whole grape powder, providing the equivalent of two servings of California table grapes, on the gut microbiome and cholesterol/bile acid metabolism in healthy adults. This study included a 4-week standardization to a low-polyphenol diet, followed by 4 weeks of 46 g of grape powder consumption while continuing the low-polyphenol diet. Compared to the baseline, 4 weeks of grape powder consumption significantly increased the alpha diversity index of the gut microbiome. There was a trend of increasing Verrucomicrobia (p = 0.052) at the phylum level, and a significant increase in Akkermansia was noted. In addition, there was an increase in Flavonifractor and Lachnospiraceae_UCG-010, but a decrease in Bifidobacterium and Dialister at the genus level. Grape powder consumption significantly decreased the total cholesterol by 6.1% and HDL cholesterol by 7.6%. There was also a trend of decreasing LDL cholesterol by 5.9%, and decreasing total bile acid by 40.9%. Blood triglyceride levels and body composition were not changed by grape powder consumption. In conclusion, grape powder consumption significantly modified the gut microbiome and cholesterol/bile acid metabolism.

3.
Neuropsychiatr Dis Treat ; 17: 3245-3253, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34754192

RESUMO

Objective: Intracerebral hemorrhage (ICH) triggers an inflammatory cascade that damages brain tissues and worsens functional outcome. S100A12 functions to promote brain inflammation. We aimed to investigate the relationship between serum S100A12 levels and functional outcome in ICH patients. Methods: Serum S100A12 levels were measured in 101 ICH patients hospitalized within 24 h after symptom onset. Poor functional outcome was defined as a modified Rankin scale of 3 or greater at 3 months after stroke. Early neurologic deterioration was defined as an increase of ≥4 points in the National Institutes of Health Stroke Scale (NIHSS) score or death at 24 hours from symptoms onset. Results: High serum S100A12 levels were independently correlated with NIHSS score (t = 5.384, P < 0.001), hematoma volume (t = 4.221, P < 0.001) and serum C-reactive protein levels (t = 5.068, P < 0.001). Serum S100A12 levels were substantially higher in patients with a poor outcome (median, 66.5 versus 37.7 ng/mL; P < 0.001) or early neurological deterioration (median, 76.5 versus 40.1 ng/mL; P < 0.001) than in the other remainders, independently predicted a poor outcome (odds ratio, 1.035; 95% confidence interval, 1.007-1.064; P = 0.015) and early neurologic deterioration (odds ratio,1.032; 95% confidence interval, 1.003-1.060; P = 0.027), and significantly discriminated a poor outcome (area under curve, 0.794; 95% confidence interval, 0.702-0.868) and early neurologic deterioration (area under curve, 0.760; 95% confidence interval, 0.664-0.839) under receiver operating characteristic curve. Conclusion: High serum S100A12 levels at admission are highly associated with the extent of inflammatory response, severity, a poor functional outcome and early neurologic deterioration in ICH patients, substantializing serum S100A12 as a promising prognostic biomarker for ICH.

4.
Plants (Basel) ; 10(10)2021 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-34685951

RESUMO

Litchi possesses unique flower morphology and adaptive reproduction strategies. Although previous attention has been intensively devoted to the mechanisms underlying its floral induction, the molecular basis of flower sex determination remains largely unknown. MADS-box genes are promising candidates for this due to their significant roles in various aspects of inflorescence and flower organogenesis. Here, we present a detailed overview of phylogeny and expression profiles of 101 MADS-box genes that were identified in litchi. These LcMADSs are unevenly located across the 15 chromosomes and can be divided into type I and type II genes. Fifty type I MADS-box genes are subdivided into Mα, Mß and Mγ subgroups, while fifty-one type II LcMADSs consist of 37 MIKCC -type and 14 MIKC *-type genes. Promoters of both types of LcMADS genes contain mainly ABA and MeJA response elements. Tissue-specific and development-related expression analysis reveal that LcMADS51 could be positively involved in litchi carpel formation, while six MADS-box genes, including LcMADS42/46/47/75/93/100, play a possible role in stamen development. GA is positively involved in the sex determination of litchi flowers by regulating the expression of LcMADS51 (LcSTK). However, JA down-regulates the expression of floral organ identity genes, suggesting a negative role in litchi flower development.

5.
Orthop J Sports Med ; 9(7): 23259671211003982, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34345631

RESUMO

Background: Calcitonin gene-related peptide (CGRP), which has been shown to play an important role in osteogenesis during fracture repair, is also widely distributed throughout the tendon and ligament. Few studies have focused on the role of CGRP in repair of the bone-tendon interface (BTI). Purpose: To explore the effect of CGRP expression on BTI healing in a rabbit partial patellectomy model. Study Design: Controlled laboratory study. Methods: A total of 60 mature rabbits were subjected to a partial patellectomy and then randomly assigned to CGRP, CGRP-antagonist, and control groups. In the CGRP-antagonist group, the CGRP receptor antagonist BIBN4096BS was administered to block CGRP receptors. The patella-patellar tendon complex was harvested at 8 and 16 weeks postoperatively and subjected to radiographic, microlaser Raman spectroscopy, histologic, and biomechanical evaluation. Results: Radiographic data showed that local CGRP expression improved the growth parameters of newly formed bone, including area and volumetric bone mineral density (P < .05 for both). Raman spectroscopy revealed that the relative bone mineral composition increased in the CGRP group compared with in the control group and the CGRP-antagonist group (P < .05 for both). Histologic testing revealed that the CGRP group demonstrated better integration, characterized by well-developed trabecular bone expansion from the residual patella and marrow cavity formation, at the 8- and 16-week time points. Mechanical testing demonstrated that the failure load, ultimate strength, and stiffness in the CGRP group were significantly higher than those in the control group (P < .05 for all), whereas these parameters in the CGRP-antagonist group were significantly lower compared with those in the control group at 16 weeks after surgery (P < .05 for all). Conclusion: Increasing the local concentration of CGRP in the early stages of BTI healing enhanced osteogenesis in a rabbit partial patellectomy model and promoted healing of the BTI injury, whereas treatment using a CGRP antagonist had the opposite effect. However, exogenous CGRP expression did not induce novel bone remolding. Clinical Relevance: CGRP may have potential as a new therapy for BTI injuries or may be added to postoperative regimens to facilitate healing.

6.
Mol Med Rep ; 24(4)2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34368884

RESUMO

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the Transwell cell migration data shown in Figs. 2C and D, and 4B and D, were strikingly similar to data appearing in different form in other articles by different authors. Owing to the fact that the contentious data in the above article had already been published elsewhere, or were already under consideration for publication, prior to its submission to Molecular Medicine Reports, the Editor has decided that this paper should be retracted from the Journal. The authors were asked for an explanation to account for these concerns, but the Editorial Office did not receive any reply. The Editor apologizes to the readership for any inconvenience caused. [the original article was published in Molecular Medicine Reports 12: 4554­4559, 2015; DOI: 10.3892/mmr.2015.3937].

7.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 37(7): 633-640, 2021 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-34140075

RESUMO

Objective To detect and analyze the plasma levels and immunoactivities of different forms of circulating-free DNA (cfDNA) in systemic lupus erythematosus (SLE) patients. Methods The study enrolled 58 patients with SLE, 66 patients with other autoimmune diseases (non-SLE) and 60 healthy individuals. Total cfDNA, exosome cfDNA and immune complex cfDNA were extracted from the plasma and detected using a fluorescence method. Overall methylation levels of cfDNA were measured. Macrophages and dendritic cells induced in vitro were co-cultured with exosomes or immune complexes derived from SLE patients pre-treated with deoxyribonuclease 1-like 3(DNASE1L3) or immunoglobulin G (IgG) specific degradation enzyme or none. Then, cytokines and cell surface activation markers were detected by the multiple fluorescent microsphere assay. Results Among the three groups, SLE patients had the highest levels of exosomes and immune complex cfDNA, followed by non-SLE patients, and no significant differences were found in the simple cfDNA. The methylation levels of different types of cfDNA (except simple cfDNA) in the plasma of SLE patients were significantly lower than that of the non-SLE group and healthy individuals. Non-SLE patients and healthy individuals presented no significant differences for the methylation levels of all cfDNA types. After DNASE1L3 and IgG enzyme treatment, the macrophages and dendritic cells stimulated by exosomes and immune complex cfDNA secreted significantly lower levels of cytokines including MIP-1a, MIP-1b, IL-4, IL-1ß, IL-2, IL-1ra, MCP-1, IL-6, IL-9, IL-8, TNF-α and IFN-α. Compared with IgG enzyme treatment, DNASE1L3-treated exosomes and immune complex induced the lower level of cytokines secretion. The expression of CD80, CD86 and CD40 on the surfaces of macrophages and dendritic cells stimulated with exosomes and immune complexes pre-treated with DNASE1L3 enzyme were significantly reduced, but the expression of CD86 on the macrophages was slightly changed. The expression of CD80, CD86 and CD40 showed no difference in the two types of cells stimulated with exosomes and immune complexes pre-treated with IgG enzymes or none, but an overall downward trend existed indeed. Conclusion Exosomes and immune complex cfDNA increase significantly in the plasma of SLE patients, and they can stimulate strong responses of macrophages and dendritic cells.


Assuntos
Ácidos Nucleicos Livres , Lúpus Eritematoso Sistêmico , Complexo Antígeno-Anticorpo , Citocinas , DNA , Humanos
8.
Oncol Rep ; 46(1)2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34080647

RESUMO

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that certain of the cell Transwell assay data in the article (featured in Figs. 3B and 6B) were strikingly similar to data that appearing in different form in another article by different authors at different research institutions, which had already been published elsewhere at the time of the present article's submission. Owing to the fact that the contentious data in the above article had already appeared in different form in another article prior to its submission to Oncology Reports, the Editor has decided that this paper should be retracted from the Journal. The authors did not reply to indicate whether or not they agreed with the retraction of the paper. The Editor apologizes to the readership for any inconvenience caused. [the original article was published on Oncology Reports 34: 399­406, 2015; DOI: 10.3892/or.2015.3986].

9.
Orthop Surg ; 13(4): 1170-1180, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33942987

RESUMO

OBJECTIVE: This study aimed to investigate the clinical effects of surgically treating lumbosacral tuberculosis with a modified posterior unilateral limited laminectomy method for debridement. METHODS: This retrospective study enrolled a total of 26 patients who were administered in our institution from January 2010 to December 2016, diagnosed with lumbosacral tuberculosis at the L5/S1 level, and underwent one-stage posterior unilateral limited laminectomy as surgical treatment for debridement, allograft of cortical bone grafting, and fixation. The erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) level, visual analog scale (VAS) score, Oswestry Disability Index (ODI), and lumbosacral angle (LA, Cobb's method) were statistically compared, and the American Spinal Injury Association Impairment (ASIA) Scale was compared between the preoperative and postoperative time points to evaluate the clinical outcomes. RESULTS: All 26 patients were observed during the follow-up period, and the mean follow-up time was 1.3 ± 0.42 years. The mean age was 56 ± 7.4 years old. The average operation time was 118.1 ± 17.5 min, and the mean bleeding volume was 513.0 ± 79.6 mL. There were no intraoperative complications or tuberculous sinus, and two cases experienced hypostatic pneumonia during hospitalization, which resolved with responsive antibiotics and symptomatic supportive treatment. At the final follow-up, there was no recurrence of tuberculosis, and the ESR (11.8 ± 1.8 mm/h) and CRP (3.0 ± 1.0 mg/L) levels in all patients had returned to normal. The patients with neurologic deficits had improved, and the mean ODI was 79.9 ± 10.6 (87-62) preoperatively and significantly decreased to 20.5 ± 5.7 (11-29) at the final follow-up (P < 0.01). ASIA scale scores were improved by 1~2 grades at the last follow-up. The patients' pain levels were significantly alleviated; the mean VAS score declined to 1.2 ± 0.4 (0-2.5) at the final follow-up compared to 7.5 ± 1.6 (6.5-8.5) preoperatively (P < 0.01). All patients achieved bony graft fusion at an average time of 6.8 ± 1.2 months. Physiological lumbar lordosis was significantly improved, and the mean LA before operation was 17.6° ± 2.1°, which was significantly different from the postoperative LA (29.3° ± 7.4°, P < 0.01) at the final follow up. The LA (27.1° ± 5.5°, P = 0.15) slightly rebounded but without significance compared to the postoperative level. CONCLUSION: Only posterior approach by unilateral limited laminectomy for debridement could be served as an effective and safe method to treat short-segment lumbosacral tuberculosis without extensive anterior sacral and gravitation abscesses.


Assuntos
Transplante Ósseo/métodos , Desbridamento/métodos , Laminectomia/métodos , Região Lombossacral/cirurgia , Fusão Vertebral/métodos , Tuberculose da Coluna Vertebral/cirurgia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
10.
Nutrients ; 13(5)2021 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-33946212

RESUMO

Mixed tree nuts (MTNs) are an excellent source of protein and healthy fat contributing to satiety. However, their relatively high caloric content might not be beneficial in a weight loss diet. The present study was designed to test whether including MTNs in a weight loss and maintenance program interferes with weight management compared to a refined carbohydrate pretzel snack (PS). We performed a randomized, controlled, two-arm study in 95 overweight individuals consuming 1.5 oz of MTNs or PS daily as part of a hypocaloric weight loss diet (-500 kcal) over 12 weeks followed by an isocaloric weight maintenance program for 12 weeks. Participants in both groups experienced significant weight loss (12 weeks: -1.6 and -1.9 and 24 weeks: -1.5 and -1.4 kg) compared to baseline in the MTN and PS groups, respectively. However, there was no difference in weight loss and other outcome parameters between the MTN and PS groups. The MTN group showed a significant increase in satiety at 24 weeks. Both groups had a decrease in diastolic blood pressure at 12 weeks. Participants in the MTN group showed significant decreases in heart rate at 4, 12, and 24 weeks. Plasma oleic acid was significantly increased at 12 and 24 weeks in the MTN group but only at 12 weeks in the PS group. Plasma MCP-1 was decreased significantly in the MTN group at 4 weeks. In summary, participants in both groups lost weight, but only the MTN intervention increased satiety at 24 weeks, enhanced retention, decreased heart rate, and increased serum oleic acid at 24 weeks.


Assuntos
Carboidratos da Dieta/administração & dosagem , Nozes , Resposta de Saciedade , Lanches , Perda de Peso , Composição Corporal , Peso Corporal , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Sobrepeso/dietoterapia , Árvores
11.
Biomed Res Int ; 2021: 6616434, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33791366

RESUMO

Objective: In this study, we aimed to identify critical genes and pathways for multiple brain regions in Parkinson's disease (PD) by weighted gene coexpression network analysis (WGCNA). Methods: From the GEO database, differentially expressed genes (DEGs) were separately identified between the substantia nigra, putamen, prefrontal cortex area, and cingulate gyrus of PD and normal samples with the screening criteria of p value < 0.05 and ∣log2fold change (FC) | >0.585. Then, a coexpression network was presented by the WGCNA package. Gene modules related to PD were constructed. Then, PD-related DEGs were used for construction of PPI networks. Hub genes were determined by the cytoHubba plug-in. Functional enrichment analysis was then performed. Results: DEGs were identified for the substantia nigra (17 upregulated and 52 downregulated genes), putamen (317 upregulated and 317 downregulated genes), prefrontal cortex area (39 upregulated and 72 downregulated genes), and cingulate gyrus (116 upregulated and 292 downregulated genes) of PD compared to normal samples. Gene modules were separately built for the four brain regions of PD. PPI networks revealed hub genes for the substantia nigra (SLC6A3, SLC18A2, and TH), putamen (BMP4 and SNAP25), prefrontal cortex area (SNAP25), and cingulate gyrus (CTGF, CDH1, and COL5A1) of PD. These DEGs in multiple brain regions were involved in distinct biological functions and pathways. GSEA showed that these DEGs were all significantly enriched in electron transport chain, proteasome degradation, and synaptic vesicle pathway. Conclusion: Our findings revealed critical genes and pathways for multiple brain regions in PD, which deepened the understanding of PD-related molecular mechanisms.


Assuntos
Encéfalo/metabolismo , Bases de Dados de Ácidos Nucleicos , Regulação para Baixo , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Regulação para Cima , Encéfalo/patologia , Humanos , Doença de Parkinson/genética , Doença de Parkinson/patologia
12.
Biomed Res Int ; 2021: 6644827, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33834070

RESUMO

Objective: This study is aimed at understanding the molecular mechanisms and exploring potential therapeutic targets for atrial fibrillation (AF) by multiomics analysis. Methods: Transcriptomics and methylation data of AF patients were retrieved from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) and differentially methylated sites between AF and normal samples were screened. Then, highly expressed and hypomethylated and lowly expressed and hypermethylated genes were identified for AF. Weighted gene coexpression network analysis (WGCNA) was presented to construct AF-related coexpression networks. 52 AF blood samples were used for whole exome sequence. The mutation was visualized by the maftools package in R. Key genes were validated in AF using independent datasets. Results: DEGs were identified between AF and controls, which were enriched in neutrophil activation and regulation of actin cytoskeleton. RHOA, CCR2, CASP8, and SYNPO2L exhibited abnormal expression and methylation, which have been confirmed to be related to AF. PCDHA family genes had high methylation and low expression in AF. We constructed two AF-related coexpression modules. Single-nucleotide polymorphism (SNP) was the most common mutation type in AF, especially T > C. MUC4 was the most frequent mutation gene, followed by PHLDA1, AHNAK2, and MAML3. There was no statistical difference in expression of AHNAK2 and MAML3, for AF. PHLDA1 and MUC4 were confirmed to be abnormally expressed in AF. Conclusion: Our findings identified DEGs related to DNA methylation and mutation for AF, which may offer possible therapeutic targets and a new insight into the pathogenesis of AF from a multiomics perspective.


Assuntos
Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/genética , Epigênese Genética , Genômica , Terapia de Alvo Molecular , Idoso , Caderinas/genética , Caderinas/metabolismo , Metilação de DNA/genética , Feminino , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Humanos , Masculino , Mutação/genética , Reprodutibilidade dos Testes , Sequenciamento Completo do Exoma
13.
Biomed Res Int ; 2021: 6653802, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33860048

RESUMO

Objective: Multiple genes have been identified to cause dilated cardiomyopathy (DCM). Nevertheless, there is still a lack of comprehensive elucidation of the molecular characteristics for DCM. Herein, we aimed to uncover putative molecular features for DCM by multiomics analysis. Methods: Differentially expressed genes (DEGs) were obtained from different RNA sequencing (RNA-seq) datasets of left ventricle samples from healthy donors and DCM patients. Furthermore, protein-protein interaction (PPI) analysis was then presented. Differentially methylated genes (DMGs) were identified between DCM and control samples. Following integration of DEGs and DMGs, differentially expressed and methylated genes were acquired and their biological functions were analyzed by the clusterProfiler package. Whole exome sequencing of blood samples from 69 DCM patients was constructed in our cohort, which was analyzed the maftools package. The expression of key mutated genes was verified by three independent datasets. Results: 1407 common DEGs were identified for DCM after integration of the two RNA-seq datasets. A PPI network was constructed, composed of 171 up- and 136 downregulated genes. Four hub genes were identified for DCM, including C3 (degree = 24), GNB3 (degree = 23), QSOX1 (degree = 21), and APOB (degree = 17). Moreover, 285 hyper- and 321 hypomethylated genes were screened for DCM. After integration, 20 differentially expressed and methylated genes were identified, which were associated with cell differentiation and protein digestion and absorption. Among single-nucleotide variant (SNV), C>T was the most frequent mutation classification for DCM. MUC4 was the most frequent mutation gene which occupied 71% across 69 samples, followed by PHLDA1, AHNAK2, and MAML3. These mutated genes were confirmed to be differentially expressed between DCM and control samples. Conclusion: Our findings comprehensively analyzed molecular characteristics from the transcriptome, epigenome, and genome perspectives for DCM, which could provide practical implications for DCM.


Assuntos
Cardiomiopatia Dilatada/genética , Epigenoma , Genoma Humano , Genômica , Transcriptoma/genética , Cardiomiopatia Dilatada/sangue , Metilação de DNA/genética , Feminino , Perfilação da Expressão Gênica , Ontologia Genética , Ventrículos do Coração/metabolismo , Ventrículos do Coração/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Anotação de Sequência Molecular , Mutação/genética , Polimorfismo de Nucleotídeo Único/genética , Mapas de Interação de Proteínas/genética , Reprodutibilidade dos Testes , Sequenciamento Completo do Exoma
14.
Environ Pollut ; 273: 116481, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33465655

RESUMO

Biochars can strongly sorb hydrophobic organic contaminants in soils. However, contribution of contaminants in different binding sites to their biodegradation in biochar-amended soils is not clear. In this work, wheat straw biochars were prepared at pyrolysis temperatures of 400 °C (BC400) and 700 °C (BC700). During a 42-day experiment, degradation rate constant of phenanthrene in soils was in the order of treatment without biochar (1.64 × 10-2 d-1) > treatment with BC700 (0.96 × 10-2 d-1) > treatment with BC400 (0.30 × 10-2 d-1). At the beginning, amendment of BC400 and BC700 reduced the rapidly desorbing fraction of phenanthrene in soils by 44.8% and 92.5%, respectively. At the end, both phenanthrene and microbial biomass highly concentrated on the biochar separated from soils. The results of a coupled model of desorption and biodegradation revealed that only phenanthrene in rapidly desorbing sites was degraded in BC400-amended soils, whereas degradation of phenanthrene in both rapidly and slowly desorbing sites occurred in BC700-amended soils, contributing 24.4% and 75.6% of the degradation, respectively. High fraction (>95%) of biodegradable phenanthrene in slowly desorbing sites was the key reason for higher biodegradation rate of phenanthrene in soils with BC700 than in soils with BC400.

15.
Nutr Res ; 85: 60-70, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33450667

RESUMO

It was our hypothesis that foods high in polyphenols and fiber have prebiotic activity. This human intervention study aimed to determine if daily consumption of freeze-dried California strawberry powder (SBP) leads to changes in the intestinal microbiota, fecal cholesterol and bile acid (BA) microbial metabolites. Fifteen healthy adults consumed a beige diet+26 g of SBP for 4 weeks, followed by 2 weeks of beige diet only. Stool samples were collected at 0, 4, and 6 weeks. Fecal microbiota was analyzed by 16S rRNA sequencing; fecal cholesterol, BA, and microbial metabolites by gas chromatography. Confirming compliance, urine concentration of pelargonidin, urolithin A glucuronide and dimethylellagic acid glucuronide were present after 4 weeks of SBP consumption. Daily SBP altered the abundance of 24 operational taxonomic units (OTUs). Comparing week 4 to baseline the most significant increases were observed for one OTU from Firmicutes\Clostridia\ Christensenellaceae\NA, one OTU from Firmicutes\ Clostridia\Mogibacteriacea\NA, one OTU from Verrucomicrobia\ Verrucomicrobiaceae\Akkermansia\Muciniphila, one OTU from Actinobacteria\ Bifidobacteriaceae\Bifidobacterium\NA, and one OTU from Bacteroidetes\Bacteroidia\ Bacteroidaceae\Bacteroides and decrease of one OTU from Proteobacteria\ Betaproteobacteria\Alcaligenaceae\Sutterella. Comparing week 4 to 6, we observed a reversal of the same OTUs from C Christensenellaceae, V muciniphilia and C Mogibacteriaceae. Fecal short chain fatty acids and most of the fecal markers including cholesterol, coprostanol, primary and secondary BAs were not changed significantly except for lithocholic acid, which was increased significantly at week 6 compared to baseline. In summary, SBP consumption increased the abundance of gut microorganisms related to lean body weight, health and longevity, and increased fecal lithocholic acid at week 6 in healthy study participants.


Assuntos
Peso Corporal , Dieta , Fragaria , Frutas , Microbioma Gastrointestinal , Longevidade , Adolescente , Adulto , Bactérias/classificação , Ácidos e Sais Biliares/análise , Colesterol/análise , Ácidos Graxos Voláteis/análise , Fezes/química , Fezes/microbiologia , Feminino , Saúde , Voluntários Saudáveis , Humanos , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Adulto Jovem
16.
Neurotherapeutics ; 18(1): 488-502, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33140235

RESUMO

Astrocytes redifferentiate into oligodendrogenesis, raising the possibility that astrocytes may be a potential target in the treatment of adult demyelinated lesion. Upon the basis of the improvement of behavior abnormality and demyelination by ethyl pyruvate (EP) treatment, we further explored whether EP affects the function of astrocytes, especially the transdifferentiation of astrocytes into oligodendrogenesis. The results showed that EP treatment increased the accumulation of astrocytes in myelin sheath and promoted the phagocytosis of myelin debris by astrocytes in vivo and in vitro. At the same time, EP treatment induced astrocytes to upregulate the expression of CNTF and BDNF in the corpus callosum and striatum as well as cultured astrocytes, accompanied by increased expression of nestin, Sox2, and ß-catenin and decreased expression of Notch1 by astrocytes. As a result, EP treatment effectively promoted the generation of NG2+ and PDGF-Ra+ oligodendrocyte precursor cells (OPCs) that, in part, express astrocyte marker GFAP. Further confirmation was performed by intracerebral injection of primary astrocytes labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). As expected, NG2+ OPCs expressing CFSE and Sox2 were elevated in the corpus callosum of mice treated with EP following transplantation, revealing that EP can convert astrocytes into myelinating cells. Our results indicate the possibility that EP lead to effective myelin repair in patients suffering from myelination deficit.Graphical Abstract The diagram of EP action for promoting myelin regeneration in CPZ model. EP promoted migration and enrichment of astrocytes to demyelinated tissue and induced astrocytes to express neurotrophic CNTF and BDNF as well as translation factor nestin, Sox2, and ß-catenin, which should contribute to astrocytes to differentiate of oligodendrogenesis. At the same time, EP promoted astrocytes to phagocytized myelin debris for removing the harmful substances of myelin regeneration.

17.
Chemosphere ; 269: 129396, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33383252

RESUMO

Metal pollutants accumulation in the scales of drinking water distribution systems presents a potential threat to water quality. Therefore, a study was carried out on the accumulation of V(V) and As(V) by cast iron pipe scales. The accumulation of V(V) and As(V) by scales and the effects of scale dosage, pH, temperature, and anion content on the accumulation process were assessed. Results showed that scales could rapidly accumulate V(V) and As(V), with maximum accumulation amounts of 3.94 mg/g and 3.90 mg/g, respectively. An increase in pH (from 3.0 to 9.0) and sulfate concentration (from 0 to 250 mg/L) decreased V(V) and As(V) accumulation by scales. Increased chloride ion concentrations (from 0 to 250 mg/L) reduced the amount of As(V) accumulated, while increasing the amount of V(V) accumulated. The V(V) and As(V) accumulation kinetics were well described by the Elovich model, with thermodynamic and accumulation isotherms showing that the accumulation process occurred via an entropic endothermic reaction. The mechanisms of accumulation of V(V) and As(V) by the scales include surface complexation, ligand exchange, electrostatic attraction and repulsion, and competitive adsorption.


Assuntos
Arsênio , Água Potável , Poluentes Químicos da Água , Purificação da Água , Adsorção , Arsênio/análise , Concentração de Íons de Hidrogênio , Ferro , Cinética , Vanádio , Poluentes Químicos da Água/análise
18.
J Hazard Mater ; 410: 124584, 2021 05 15.
Artigo em Inglês | MEDLINE | ID: mdl-33248824

RESUMO

This study investigated the impact of wheat straw biochars pyrolyzed at temperatures of 100-700 â„ƒ (BC100-BC700) on biodegradation of phenanthrene in soils. During a 42-day experiment, biochar amendment reduced the biodegradation ratio of phenanthrene in soils by no change-77.0%. The biodegradation ratio decreased with the increase of pyrolysis temperature from 100 to 400 â„ƒ and then increased with the increase of pyrolysis temperature from 400 to 700 â„ƒ, exhibiting a U-shape. Meanwhile, desorbing fraction of phenanthrene extracted by n-butanol declined with increasing pyrolysis temperature. Biochar-derived dissolved organic carbon (DOC) obviously influenced the soil DOC contents which were negatively correlated with the total relative abundances of dominant polycyclic aromatic hydrocarbon (PAH)-degraders. These results indicated that in soils amended with biochars pyrolyzed at low temperatures (i.e. 100-400 â„ƒ), both the reduced bioavailability of phenanthrene and the reduced PAH-degrader abundance resulted in decreasing phenanthrene degradation with pyrolysis temperature. In soils amended with biochars pyrolyzed at high temperatures (i.e. 500-700 â„ƒ; HT-biochars), two possible reasons contribute to increasing phenanthrene degradation with pyrolysis temperature: (1) high sorbed-phenanthrene concentration due to large specific surface area and high aromaticity of the biochars, and (2) the increased dominant PAH-degrader abundance for the removal of sorbed-phenanthrene due to the impact of HT-biochars on soil properties (mainly on DOC content).


Assuntos
Microbiota , Fenantrenos , Poluentes do Solo , Disponibilidade Biológica , Carvão Vegetal , Solo , Poluentes do Solo/análise , Temperatura
19.
Front Hum Neurosci ; 14: 590815, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33240068

RESUMO

Epilepsy is a serious hazard to human health. Minimally invasive surgery is currently an extremely effective treatment to refractory epilepsy. However, it is challenging to localize the lesion for most patients because they are MRI negative. The identification of epileptic foci in local brain region will be helpful to the localization of epileptic foci because we can infer whether there is a lesion from the results of the classification. For the sake of simplicity and the data we collected, only the hippocampus was segmented as a local brain region and classified in this paper. We recruited 59 children with hippocampus epilepsy and 70 age- and sex-matched normal controls, and diffusion kurtosis images (DKI) for all subjects were collected because DKI can understand the pathological changes of local tissues and other regions of epileptic foci at the molecular level. Then, a mask of hippocampus was made to segment the hippocampus of FA, MD, and MK images for all subjects, which are the parameter images of DKI and were used to perform the independent-sample t-test and the classification task. At last, a convolutional neural network (CNN) based on transfer learning technique was developed to extract features of FA, MD, MK, and the fusion of FA and MK, and support vector machine was employed to classify epilepsy and normal control. Finally, the classifier produced 90.8% accuracy for patient vs. normal controls. Experimental results showed that the features extraction based on CNN is very effective, and the high accuracy of classification means that FA and MK are two remarkable features to identify epilepsy, which indicates that DKI images can act as an important biomarker for epilepsy from the point of view of clinical diagnosis.

20.
Front Aging Neurosci ; 12: 206, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33101003

RESUMO

Mild cognitive impairment (MCI) is a clinical state with a high risk of conversion to Alzheimer's Disease (AD). Since there is no effective treatment for AD, it is extremely important to diagnose MCI as early as possible, as this makes it possible to delay its progression toward AD. However, it's challenging to identify early MCI (EMCI) because there are only mild changes in the brain structures of patients compared with a normal control (NC). To extract remarkable features for these mild changes, in this paper, a multi-modality diagnosis approach based on deep learning is presented. Firstly, we propose to use structure MRI and diffusion tensor imaging (DTI) images as the multi-modality data to identify EMCI. Then, a convolutional neural network based on transfer learning technique is developed to extract features of the multi-modality data, where an L1-norm is introduced to reduce the feature dimensionality and retrieve essential features for the identification. At last, the classifier produces 94.2% accuracy for EMCI vs. NC on an ADNI dataset. Experimental results show that multi-modality data can provide more useful information to distinguish EMCI from NC compared with single modality data, and the proposed method can improve classification performance, which is beneficial to early intervention of AD. In addition, it is found that DTI image can act as an important biomarker for EMCI from the point of view of a clinical diagnosis.

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