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1.
J Hazard Mater ; 441: 129824, 2023 01 05.
Artigo em Inglês | MEDLINE | ID: mdl-36087529

RESUMO

The aerobic, lincomycin-degrading bacterial strain Conexibacter sp. LD01, belonging to the phylum Actinobacteria, was isolated from activated sludge. Both second- and third-generation sequencing technologies were applied to uncover the genomic characterization and high-quality genome with 99.2% completeness and 2.2% contamination was obtained. The biodegradation kinetics of lincomycin fit well with the modified Gompertz model (R2 > 0.97). Conexibacter sp. LD01 could subsist with lincomycin as the sole source of carbon, nitrogen, and energy. When 500 mg/L of glucose was added as a co-substrate, the biodegradation rate improved significantly, whereas the addition of 500 mg/L sodium pyruvate had a slight inhibitory effect. Ammonia nitrogen was the best nitrogen source for Conexibacter sp. LD01 when growing and degrading lincomycin. In total, 17 metabolic products consisting of nine novel products were detected, and five biodegradation pathways, including N-demethylation, breakage of the amido bond, sulfoxidation, and oxidation of the pyrrolidine ring and propylamino chain, were proposed. This study significantly expands our understanding of the functional microorganisms and mechanism involved in lincomycin biodegradation at the phylum level.


Assuntos
Lincomicina , Esgotos , Amônia/metabolismo , Bactérias/metabolismo , Biodegradação Ambiental , Carbono/metabolismo , Genômica , Glucose/metabolismo , Cinética , Nitrogênio/metabolismo , Piruvatos , Esgotos/química , Sódio
2.
J Ethnopharmacol ; 300: 115699, 2023 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-36113679

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Periodontal disease is a complex inflammatory disease that seriously affects peoples' lives. Scutellaria radix (SR) is traditionally used as a folk medicine to clear away heat and dampness, purge fire and detoxification. Although it has been extensively used as a medicinal plant to treat a variety of inflammatory illnesses, the efficacy and active ingredient for topical administration in the treatment of periodontitis is unknown. AIM OF STUDY: The aim of this study was to screen and validate the active ingredients in SR for the prevention and treatment of periodontitis. MATERIALS AND METHODS: A ligature-induced periodontitis in rats was used to investigate the efficacy of topical administration of SR for the treatment of periodontitis, and the active fraction was screened after separation of the aqueous extract of SR into fractions of different polarities using a lipopolysaccharide (LPS)-induced cell model. Chromatographic fingerprints were established for 18 batches of SR by high performance liquid chromatography. The potential active components were screened using spectral effect relationship analysis and the target cell extraction method. RESULTS: SR has good efficacy in the topical treatment of periodontitis, according to animal experiments. Five active ingredients were screened out and their anti-inflammatory activity was confirmed in vitro. CONCLUSION: The main active compounds in the treatment of periodontitis via topical administration of SR were found and this provides an experimental basis for further studies on the pharmacodynamic material basis of SR, as well as reference for the comprehensive evaluation of SR quality and the development of substitute resources.


Assuntos
Periodontite , Scutellaria baicalensis , Administração Tópica , Animais , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Lipopolissacarídeos , Periodontite/tratamento farmacológico , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Ratos , Scutellaria baicalensis/química
3.
Artigo em Inglês | MEDLINE | ID: mdl-36376170

RESUMO

PURPOSE: Numerous studies suggested methylation modifications play an important role in upper tract urothelial carcinoma (UTUC), but few have depicted DNA methylation architecture on the pathological process of UTUC. We aimed to better understand the pathogenesis of UTUC and provide precision medicine references when managing UTUC patients. METHODS: PubMed, Cochrane Library, EMBASE, and Scopus were searched for UTUC until December 31, 2020. Methodological quality assessment was conducted according to NIH recommendations. Meta-analysis was conducted to assess the prognostic effect of methylated genes. Kaplan-Meier survival analyses were performed to validate methylated genes and cytosine-phosphate-guanine (CpG) sites. RESULTS: Eleven studies (3619 patients) were eligible to investigate 12 methylated genes and 10 CpGs. The quality of all the studies was fair to good. Meta-analysis found the pooled effect of eligible methylated genes had a low risk of tumor recurrence (HR = 0·67; 95% CI: 0·51-0·87; P = ·003), but a high risk of tumor progression (HR = 1·60; 95% CI: 1·17-2·18; P = ·003) and cancer-specific mortality (HR = 1·35; 95% CI: 1·06-1·72; P = ·01). For individual methylation status of GDF15, HSPA2, RASSF1A, TMEFF2, and VIM, the pooled effect of each gene was found pleiotropic on both diagnosis and prognosis. Survival analysis suggested higher methylation of SPARCL1 had a better disease-specific survival (P = ·048). CONCLUSION: We combined meta-analysis and Kaplan-Meier survival analysis using the most updated evidence on the methylation of UTUC. Candidate biomarkers with essential diagnosis and prognosis function might provide precision medicine references for personalized therapies.

4.
Pathogens ; 11(11)2022 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-36365016

RESUMO

Burkholderia glumae is an important rice pathogen, thus the genomic and evolutionary history may be helpful to control this notorious pathogen. Here, we present two complete genomes of the B. glumae strains HN1 and HN2, which were isolated from diseased rice seed in China. Average nucleotide identity (ANI) analysis shows greater than 99% similarity of the strains HN1 and HN2 with other published B. glumae genomes. Genomic annotation revealed that the genome of strain HN1 consists of five replicons (6,680,415 bp) with an overall G + C content of 68.06%, whereas the genome of strain HN2 comprises of three replicons (6,560,085 bp) with an overall G + C content of 68.34%. The genome of HN1 contains 5434 protein-coding genes, 351 pseudogenes, and 1 CRISPR, whereas the genome of HN2 encodes 5278 protein-coding genes, 357 pseudogenes, and 2 CRISPR. Both strains encode many pathogenic-associated genes (143 genes in HN1 vs. 141 genes in HN2). Moreover, comparative genomic analysis shows the extreme plasticity of B. glumae, which may contribute to its pathogenicity. In total, 259 single-copy genes were affected by positive selection. These genes may contribute to the adaption to different environments. Notably, six genes were characterized as virulence factors which may be an additional way to assist the pathogenicity of B. glumae.

5.
J Cardiovasc Dev Dis ; 9(11)2022 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-36354768

RESUMO

BACKGROUND: Brugada syndrome (BrS) is an inheritable arrhythmia syndrome that can lead to sudden cardiac death in patients while the heart structure is normal. However, the genetic background of more than 65% of BrS probands remains unclear. OBJECTIVES: The purpose of this study is to report the variant spectrum in a Chinese cohort with suspected BrS and to analyze their distinct clinical and electrocardiographic features. METHODS: Patients with suspected BrS from Tongji Hospital between 2008 and 2021 were analyzed retrospectively. RESULTS: A total of 79 probands were included in this study. Patients with type 1 BrS electrocardiogram (ECG) had a prolonged QRS duration compared to patients with type 2/3 BrS ECG. Of them, 59 probands underwent genetic testing. Twenty-five patients (42.37%) showed abnormal genetic testing results, and eight of them (13.56%) carried pathogenic/likely pathogenic (P/LP) mutations. Mutation carriers presented much more prominent depolarization and repolarization abnormalities than non-carriers, including a prolonged P-wave duration, QRS duration, QTc interval, decreased QRS amplitude, and deviation of the electrocardiographic axes (T-wave axis and R-wave axis). Furthermore, our study identified four novel P/LP mutations: Q3508X in TTN, A990G in KCNH2, G1220E, and D372H (in a representative pedigree) in SCN5A. CONCLUSIONS: Our study showed the variant spectrum of a suspected Chinese BrS cohort, and we identified four novel P/LP mutations in TTN, KCNH2, and SCN5A.

6.
Sci Rep ; 12(1): 19312, 2022 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-36369267

RESUMO

The common final pathway to blindness in many forms of retinal degeneration is the death of the light-sensitive primary retinal neurons. However, the normally light-insensitive second- and third-order neurons persist optogenetic gene therapy aims to restore sight by rendering such neurons light-sensitive. Here, we investigate whether bReaChES, a newly described high sensitivity Type I opsin with peak sensitivity to long-wavelength visible light, can restore vision in a murine model of severe early-onset retinal degeneration. Intravitreal injection of an adeno-associated viral vector carrying the sequence for bReaChES downstream of the calcium calmodulin kinase IIα promoter resulted in sustained retinal expression of bReaChES. Retinal ganglion cells (RGCs) expressing bReaChES generated action potentials at light levels consistent with bright indoor lighting (from 13.6 log photons cm-2 s-1). They could also detect flicker at up to 50 Hz, which approaches the upper temporal limit of human photopic vision. Topological response maps of bReaChES-expressing RGCs suggest that optogenetically activated RGCs may demonstrate similar topographical responses to RGCs stimulated by photoreceptor activation. Furthermore, treated dystrophic mice displayed restored cortical neuronal activity in response to light and rescued behavioral responses to a looming stimulus that simulated an aerial predator. Finally, human surgical retinal explants exposed to the bReaChES treatment vector demonstrated transduction. Together, these findings suggest that intravitreal gene therapy to deliver bReaChES to the retina may restore vision in human retinal degeneration in vivo at ecologically relevant light levels with spectral and temporal response characteristics approaching those of normal human photopic vision.


Assuntos
Degeneração Retiniana , Camundongos , Humanos , Animais , Channelrhodopsins/genética , Channelrhodopsins/metabolismo , Degeneração Retiniana/genética , Degeneração Retiniana/terapia , Degeneração Retiniana/metabolismo , Optogenética/métodos , Opsinas de Bastonetes/metabolismo , Células Ganglionares da Retina/metabolismo
7.
Nat Commun ; 13(1): 6821, 2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36357428

RESUMO

The rapid development of flexible electronics and soft robotics has an urgent demand for materials with wide-range switchable stiffness. Here, we report a polymer network that can isochorically and reversibly switch between soft ionogel and rigid plastic accompanied by a gigantic stiffness change from about 600 Pa to 85 MPa. This transition is realized by introducing polymer vitrification to regulate the liquid-liquid phase separation, namely the Berghmans' point in the phase diagram of binary gel systems. Regulating the Lewis acid-base interactions between polymer and ionic liquids, the stiffness-changing ratio of polymer network can be tuned from 10 to more than 105. These wide-range stiffness-changing ionogels show excellent shape adaptability and reconfigurability, which can enhance the interfacial adhesion between ionogel and electrode by an order of magnitude and reduce interfacial impedance by 75%.

8.
Reproduction ; 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36342661

RESUMO

Intrauterine adhesions (IUA) is a common gynecological disease and is a leading cause of female infertility. Mechanical or infectious damage to the endometrial basal layer can lead to endometrial fibrosis, which is the most common cause of IUA. Endoplasmic reticulum stress (ERS), the transforming growth factor beta signaling pathway (TGF-ß/SMAD) and epithelial-mesenchymal transition (EMT) are important factors promoting endometrial fibrosis. The purpose of this study was to determine the up- and downstream regulatory relationships of the above three in the process of endometrial fibrosis. The rat IUA model was induced by double injury method and prophylactic injection of the ERS inhibitor 4-phenylbutyric acid (4-PBA) was given in vivo. The ERS activator tunicamycin and the TGF-ß/SMAD pathway inhibitor A 83-01 were used in human endometrial epithelial cells (HEECs) in vitro. Masson's trichrome, Sirius red staining, immunohistochemistry, immunofluorescence and west-ern blot analyses were used to determine ERS, TGF-ß/SMAD pathway, EMT and fibrosis markers in the uterine tissue and HEECs of the different treatment groups. In animal experiments, ERS and the TGF-ß/SMAD pathway had been activated and EMT occurred in an in vivo model of IUA, but suppressed in animals treated with prophylactic 4-PBA. In vitro experiments, tunicamycin-treated HEECs had increased the activation of ERS, the abundance of TGF-ß/SMAD pathway and fibrosis markers while EMT occurred, but the TGF-ß/SMAD pathway and EMT were significantly inhibited in the tunicamycin+A 83-01 group. Our data suggest that increased ERS can induce EMT and promote endometrial fibrosis through the TGF-ß/SMAD pathway.

9.
Clin Med Insights Oncol ; 16: 11795549221134832, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36387611

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common and deadly malignancies worldwide. Approximately, 80% of patients are initially diagnosed at intermediate or advanced stages, which means that curative therapies are unable to be performed. In most cases, systemic treatment is ineffective, especially when conventional cytotoxic agents are used. Sorafenib has been the only systemic agent proven to be effective in treating advanced HCC for over a decade. The rapid development of immunotherapy has remarkably revolutionized the management of advanced HCC. Besides, the combination of immunotherapy with molecular targeted agents or locoregional treatments is emerging as an effective tool for enhancing immunity. In the review, an overview of immunotherapy and its combination therapies for HCC is presented.

10.
BMC Psychiatry ; 22(1): 678, 2022 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-36329443

RESUMO

BACKGROUND: The 2020 coronavirus pandemic (COVID-19) has been raging for more than 20 months, putting significant strain on public health systems around the world. Despite the fact that the pandemic has been effectively managed in certain countries, regional outbreaks and viral mutations continue to pose a threat to people's lives. The likelihood of post-pandemic changes in people's psychological situations warrants more investigation. DESIGN AND PARTICIPANTS: This study was conducted in the context of another outbreak in Zhangjiajie, China, respondents (infected patients, healthy population) were required to complete self-administered questions and standardized questionnaires, including the patient health questionnaire-9 (PHQ-9), the generalized anxiety disorder-7 (GAD-7), and the Brief Illness Perception Questionnaire (BIPQ). MEASURES: We conducted an anonymous questionnaire survey of infected patients (excluding critically ill patients) in the confirmed COVID-19 ward of Zhangjiajie City People's Hospital's East Hospital from August 14 to 24, 2021, and used convenience sampling to survey medical staff and the general public to assess the psychological reactions of different populations during the delta variant outbreak pandemic. Differences in anxiety and depression severity were compared between groups, with logistic regression models constructed to explore potential factors associated with scoring clinical significant levels of depression and/or anxiety. RESULTS: There is no significant difference (p value = 0.228) between anxiety and depression in patients (n = 53), general public (n = 97), medical personnel (n = 103), and support workers (n = 65). Females reported higher scores on the GAD-7 and the BIPQ, reduced communication with family and friends appeared to be a risk factor for clinically significant anxiety and depression. CONCLUSIONS: There were no significant differences in anxiety and depression across populations explored in this study, but females had higher anxiety and illness perception than males, and effective communication may help improve mental health.


Assuntos
COVID-19 , Pandemias , Masculino , Feminino , Humanos , SARS-CoV-2 , Depressão/psicologia , Ansiedade/psicologia , Transtornos de Ansiedade/epidemiologia , Surtos de Doenças , Inquéritos e Questionários , China/epidemiologia , Mutação
11.
Small ; : e2205697, 2022 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-36408922

RESUMO

Optical encryption technologies are widely used in information security, whereas the technology with one single optical secret key can be easily cracked. Here, a triple encryption is reported, which hides patterned information in excitation-dependent allochroic materials with long afterglow, enhancing the security level. The allochroic materials are based on a uniaxial co-assembly structure of cellulose nanocrystals (CNCs) and silica. The assembled CNCs present blue emission with quantum yield of 19.8% under 367 nm UV radiation. The blue emission is maintained in the inverse structure when CNCs are calcinated and converted to carbon dots (CDs). The inverse uniaxial-assembly structure improves the CD emission by 6.7 times. The assembly structure can even improve the phosphorescence of CDs, leading to excellent excitation-dependent allochroic properties. Specifically, the materials maintain a cyan long afterglow luminescence at 480 nm after removing 365 nm UV light, whose lifetime is 0.492 s. Changing the excitation wavelength to 254 nm, a UV emission at 343 nm can be obtained, alongside a blue long afterglow luminescence of 420 nm, whose lifetime is 1.574 s. Combining with blue afterglow materials, optical encryption labels are prepared, which hide different patterned information in three scenarios: natural light, UV light, and afterglow luminescence.

12.
Int J Biol Macromol ; 2022 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-36403772

RESUMO

Benign prostatic hyperplasia (BPH) is a common disease among aging males. We obtained BPH transcriptional signatures by high-throughput RNA sequencing analysis. Accordingly, we determined the differentially expressed RNAs (DERNAs) between BPH tissues and normal prostate tissues. WebGestalt and R package (clusterprofiler) was used to enrichment analysis. Clinical correlations were analyzed using Spearman's coefficient. TargetScan, ENCORI, miRNet, and miRDB databases were used to predict targets' relationships in ceRNA networks. Immunofluorescence staining and qRT-PCR analyses were performed to validate the findings. Microarray analysis of the datasets showed 369 DElncRNAs, 122 DEpseudogenes, 6 DEmiRNAs and 1358 DEmRNAs. DEmRNAs were particularly enriched in the autophagy-related pathways. Following the screening of DEmRNAs and autophagy-related genes (ARGs), 50 DEARGs were selected. MCODE analysis on Cytoscape was performed for the 50 DEARGs, and 3 hub genes (ATF4, XBP1, and PPP1R15A) were obtained. Spearman's correlation analysis showed that the mRNA expression of XBP1 correlated positively with age, total score, and storage score, but negatively with the maximum flow rate. Subsequently, the pseudogene/lncRNA- hsa-miR-222-3p-XBP1 pathway was identified. Our findings elucidate that the pseudogene/lncRNA-hsa-miR-222-3p-XBP1 pathway may play a regulatory role in the occurrence of BPH through autophagy.

13.
Glob Soc Welf ; : 1-11, 2022 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-36337380

RESUMO

Background: As one of the fastest growing community-based financial capability approaches, village savings and loan group (VSLG) is an organized group and formal entity that creates opportunities for participants to save and access financial assets. VSLG has potentially positive impacts on increasing women's financial resources and social support and further improves their mental health. Participation in a VSLG not only increases women's opportunities for asset-building and income generation, but also facilitates trust and promotes social capital development. However, few studies have examined the association between the VSLG participation and women's depression status. To fill the knowledge gap, we examined the association between the VSLG participation and depressive symptoms among low-income women in Mozambique. Methods: The data was collected as part of the VSLG program evaluation. The study applied a posttest-only comparison group quasi-experimental design and sampled female VSLG participants and non-participants from three sub-villages in the Sofala province, Mozambique. A multi-stage sampling technique was employed, and a total of 205 women were randomly selected, including 105 VSLG participants and 100 non-participants. Depressive symptoms were measured using the short version of the Edinburgh Depression Scale (EDS) with a summative score ranging from 7 to 28. Using the cutoff value at a score of 14, we created a dichotomous depression indicator. Linear and logit models were used to examine the associations of the VSLG participation and the participation duration with the depression score and the presence of depression controlling for demographic variables. Results: The VSLG participants had a statistically lower mean depression score of 12.2 (SD = 4.4) compared to non-participants (15.0, SD = 4.0, p < .001). The regression analysis suggested that the VSLG participants had a mean depression score of 2.7 lower than the non-participants (p < .001). Nearly 60% of the non-participants reported the presence of depression; however, this percentage is 31% for participants (p < .001). The multivariate logit model indicated the odds of the presence of depression for participants are .34 of that for non-participants. Similar results were obtained when the VSLG program duration was used as an independent variable. Conclusions: Study findings showed a positive association between the VSLG participation and women's mental health. Future research should further explore the intervention mechanisms and assess how the VSLG participation affects women's mental health. Findings also provided important insights into developing community-based financial capability interventions to improve low-income women's mental health.

14.
Plant Cell Environ ; 2022 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-36358019

RESUMO

Wheat is a staple upland crop grown worldwide, including in saline-alkali soils with a high pH and excess salt content. Wheat plants are thus ubiquitously simultaneously exposed to salinity (NaCl) and limited iron (Fe) availability caused by soil saline-alkalization. Seminal root growth in cereal crops is fundamental for the early establishment of seedlings and grain yield. Through this study, we found that both low Fe and NaCl severely inhibited the growth of seminal roots in wheat (Triticum aestivum L.) seedlings; however, sufficient Fe (+Fe) caused greater cessation of seminal root growth than low Fe (-Fe) under salt stress. Low Fe improved the division activity of the meristematic zone but it did not alter the cell size in the elongation region of the root tips compared with sufficient Fe under salt stress. Foliar Fe spray and split-root experiments showed that the low Fe-mediated alleviation of the salinity-induced growth cessation of seminal roots was dependent on local low Fe signals in the roots. Ionomics combined with TEM/X-ray few differences in the root Na+ uptake and vacuolar Na+ sequestration between sufficient Fe and low Fe conditions under salt stress. Phytohormone profiling and high-resolution metabolite fingerprints revealed salinity-induced overaccumulation of ACC/ethylene and tryptophan/auxin in the roots under sufficient Fe than under low Fe. Furthermore, genome-wide transcriptomics-assisted differential gene expression, exogenous application of various pharmacological inhibitors and the root growth performance of transgenic wheat plants revealed that TaPIN2-7D mediated the rootward auxin efflux and was responsible for the low Fe-mediated amelioration of the salinity-induced growth cessation of seminal roots. Our findings will provide novel insights into the understanding of Fe involved nutrient interactions and the modulation of root growth under salt stress orchestration the ethylene-auxin homeostasis in crop species. This article is protected by copyright. All rights reserved.

15.
Chem Commun (Camb) ; 2022 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-36383163

RESUMO

Herein, we developed a colorimetric and electrochemical integrated dual-mode assay for glucose detection by utilizing CoOOH@Cu nanosheets as peroxidase mimetics. With the advantages of self-calibration, sensitivity and lower sample cost, this designed dual-mode assay offers great potential in blood glucose analysis.

16.
Sci Total Environ ; 858(Pt 3): 159984, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36356751

RESUMO

Anaerobic digestion of lignocelluloses for biogas production is greatly restricted by the poor biomass degradability. Herein, a novel approach is suggested to enhance the energy recovery from rice straw through a two-step conversion using eggshell-based catalytic pyrolysis followed by biochar-based anaerobic co-digestion. Pyrolysis with eggshell significantly enhanced the crude bio-oil yield by 4.6 %. Anaerobic digestion of rice straw using 4 g L-1 of rice straw biochar (RB) showed the highest recorded biogas yield of 503.7 L kg-1 VS, with 268.6 L kg-1 VS biomethane yield. However, 4 g L-1 of calcium-enriched eggshell rice straw biochar (ERB) enhanced the biomethane yield to 281.8 L kg-1 VS, which represented 95.6 % higher than the control. It was attributed to enhancement of biomethanation, which resulted in 74.5 % maximum recorded biomethane content at the 7th day of anaerobic digestion. Microbial analysis confirmed that Methanosarciniales was the most dominant Archael group in the control (14.84 %), which increased sharply to 73.91 % and 91.66 % after addition of 4 g L-1 RB and ERB, respectively. The suggested route enhanced the energy recovery in the form of bio-oil and biomethane by 41.6 %.

17.
Nano Lett ; 22(22): 8966-8974, 2022 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-36374184

RESUMO

Softness, adhesion, stretchability, and fast recovery from large deformations are essential properties for conductive elastomers that play an important role in the development of high-performance soft electronics. However, it remains an ongoing challenge to obtain conductive elastomers that combine these properties. We have fabricated a super soft (Young's modulus 2.3-12 kPa), highly stretchable (up to 1500% strain), and underwater adhesive silicone conductive elastomer composite (SF-C-PDMS) by incorporating dimethyl silicone oil as a lubricating agent in a cross-linked molecular network. The resultant SF-C-PDMS not only exhibits superior softness but also can readily recover after a strain of 1000%. The initial resistance only decreases by 8% after 100000 cycles of tensile fatigue test (100% strain, 0.5 Hz, 15 mm/s). This multifunctional silicone conductive elastomer composite is obtained in a one-step preparation at room temperature using commercially available materials. Moreover, we illustrate the capabilities of this composite in motion sensing.

18.
BMC Pulm Med ; 22(1): 428, 2022 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-36402998

RESUMO

BACKGROUND: This study aimed to evaluate the diagnostic accuracy of pleural fluid (PF) lactate dehydrogenase (LDH) to adenosine deaminase (ADA) (LDH/ADA) ratio for tuberculous pleural effusion (TPE). Especially to explore whether the LDH/ADA ratio provides added diagnostic value to ADA. METHODS: The diagnostic accuracy of PF LDH/ADA ratio and ADA for TPE was evaluated in two cohorts, named the BUFF (Biomarkers for patients with Undiagnosed pleural eFFusion) cohort (62 with TPE and 194 with non-TPE) and the SIMPLE (a Study Investigating Markers in PLeural Effusion) cohort (33 with TPE and 177 with non-TPE). Receiver operating characteristic (ROC) curve and decision curve were used to measure the diagnostic accuracy of the PF LDH/ADA ratio. The added diagnostic value of the LDH/ADA ratio to ADA was evaluated with net reclassification improvement (NRI) and integrated discrimination improvement (IDI). RESULTS: The area under the ROC curves (AUCs) of PF ADA and LDH/ADA ratio in the BUFF cohort were 0.76 and 0.74, respectively. In the SIMPLE cohort, the AUCs of PF ADA and LDH/ADA ratio were 0.80 and 0.85, respectively. The decision curves of PF LDH/ADA and ADA were close in both the BUFF and SIMPLE cohorts. The NRI and IDI analyses did not reveal any added diagnostic value of LDH/ADA to ADA. CONCLUSIONS: PF LDH/ADA ratio has moderate diagnostic accuracy for TPE. It does not provide added diagnostic value beyond ADA. The current evidence does not support LDH/ADA ratio for diagnosing TPE.


Assuntos
Derrame Pleural , Tuberculose Pleural , Humanos , Adenosina Desaminase , Tuberculose Pleural/diagnóstico , L-Lactato Desidrogenase , Derrame Pleural/diagnóstico , Exsudatos e Transudatos , Biomarcadores
19.
BMC Plant Biol ; 22(1): 502, 2022 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-36289462

RESUMO

BACKGROUND: Soil salinization has become a global problem restricting the seed yield and quality of crops, including wheat (Triticum aestivum L.). Salinity significantly alters plant morphology and severely disrupts physiological homeostasis. Salt tolerance of wheat has been widely studied whereas core ion transporters responsive to salt stress remain elusive. RESULTS: In this study, the wheat seedlings were subjected to salinity toxicity for morpho-physiological and transcriptomic analysis of wheat salt tolerance. There was a inversely proportional relationship between salt concentrations and morpho-physiological parameters. Under the condition of 100 mM NaCl, the H2O2, O2-, MDA content and membrane permeability were significantly increased whereas the chlorophyll content was markedly decreased. Under salt stress, a larger proportion of Na+ was partitioned in the roots than in the shoots, which had a lower Na+/K+ ratio and proline content. Salt stress also obviously affected the homeostasis of other cations. Genome-wide transcriptomic analysis showed that a total of 2,807 and 5,570 differentially expressed genes (DEGs) were identified in the shoots and roots, respectively. Functionality analysis showed that these DEGs were mainly enriched in the KEGG pathways related to carbon metabolism, phenylalanine, and amino acid biosynthesis, and were primarily enriched in the GO terms involving proline metabolism and redox processes. The Na+ transporter genes were upregulated under salt stress, which repressed the gene expression of the K+ transporters. Salt stress also significantly elevated the expression of the genes involved in osmoregulation substances biosynthesis, and obviously affected the expression profiling of other cation transporters. Co-expression network analysis identified TaNHX6-D5/TaNHX4-B7 and TaP5CS2-B3 potentially as core members regulating wheat salt tolerance. CONCLUSIONS: These results might help us fully understand the morpho-physiological and molecular responses of wheat seedlings to salt stress, and provide elite genetic resources for the genetic modification of wheat salt tolerance.


Assuntos
Plântula , Triticum , Triticum/metabolismo , Plântula/genética , Plântula/metabolismo , Osmorregulação , Peróxido de Hidrogênio/metabolismo , Cloreto de Sódio/metabolismo , Estresse Salino/genética , Salinidade , Sódio/metabolismo , Clorofila/metabolismo , Prolina/metabolismo , Carbono/metabolismo , Nutrientes , Solo , Fenilalanina/metabolismo , Aminoácidos/metabolismo , Estresse Fisiológico/genética
20.
Dalton Trans ; 51(42): 16158-16169, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36205259

RESUMO

The reaction of a macrocyclic ligand 1,4,7-triazacyclononane-1,4,7-tripropionic acid (tacntpH3) with Ni(II)/Co(II) sources in the presence or absence of lanthanide cations yielded a series of doubly mononuclear ionic complexes (H3O+)[LnIII(H2O)8][NiII(tacntp)]4 (1-NiLn, Ln = La, Ce, Yb) and a mononuclear Co(III) complex [CoIII(tacntp)]·4H2O (2-Co) incorporating transition metal centers enveloped by both an azamacrocycle ring and pendant carboxylate groups. Either of the two preorganized mononuclear species [MII/III(tacntp)]-/0 was taken as a tripodal 3d metalloligand for the further assembly of modular architectures. Two pentanuclear metallocage-[Ln(NO3)6]3- complexes [NiII5(tacntp)2(H2O)12][LnIII(NO3)6]Cl·2H2O (3-NiLn, Ln = La, Ce), one nonanuclear metallocage [NiII9(tacntp)4(H2O)18](ClO4)6·10H2O (4-Ni), and two types of 2D layered heterometallic 3d-4f coordination networks ((H3O+)[NiII2YbIII(tacntp)2](ClO4)2·3H2O (5-NiYb) and [CoIII2TbIII(tacntp)2(H2O)3](ClO4)·Cl2·5H2O (6-CoLn, Ln = La, Eu, Tb, Dy)) differing largely in their weaving architecture were controllably prepared via the precise regulation of multiple reaction parameters. Furthermore, the time-dependent evolution of metalloligand-derived species was tracked via electrospray ionization-mass spectrometry, to elucidate the modular assembly pathway for the nonanuclear Ni(II) metallocage 4-Ni. Analysis of the structural details and the assembly procedures of the modular architectures revealed that the pH, metalloligand size, and the type of metal bound to the metalloligand considerably influence the structural evolution from mononuclear metalloligands to cage-like and polymeric coordination architectures. Magnetic property studies disclosed that pendant syn-anti carboxylate groups favor a weak ferromagnetic exchange between the adjacent Ni(II) ions within the metallocages of 3-NiLa and 4-Ni but facilitate an antiferromagnetic coupling between the alternating Ni(II) and Yb(III) centers in 5-NiYb.

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