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1.
Inflammopharmacology ; 28(1): 19-37, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31894515

RESUMO

Cardiovascular disease (CVDs) is a chronic disease with the highest morbidity and mortality in the world. Previous studies have suggested that preventing inflammation serves an efficient role in protection against cardiovascular diseases. Modulation of IKK-ß activity can be used to treat and control CVDs associated with chronic inflammation, which targets the phosphorylation of IκB following the release of the RelA complex, and then translocates to the nucleus, eventually triggering the transcription of several genes that induce chemokines, cytokines, and adhesion molecules. Most importantly, the IκB kinase (IKK) complex is involved in transcriptional activation by phosphorylating the inhibitory molecule IkBα, enabling activation of NF-κB. Phenolic compounds possess cardioprotective potential that may be related to modulating inflammatory responses involved in CVDs. The SystemsDock analysis was used to explore whether 38 active compounds inhibit IKK-ß activity based on literature. Docking results showed that the top docking score of three chemical compounds were icariin, salvianolic acid B, and plantainoside D in all compounds. Icariin, salvianolic acid B, and plantainoside D are the most promising IKKß inhibitors. These phytochemicals could be helpful to find the lead compounds on designing and developing novel cardioprotective agents.

2.
Chin J Integr Med ; 2019 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-30623345

RESUMO

OBJECTIVE: To investigate the inhibitory effect of Linggui Zhugan Decoction (LZD, ) on the ventricular remodeling (VR) after acute myocardial infarction (AMI) and related mRNA and proteins expression in transforming growth factor-beta 1 (TGF-ß1)/Smad signaling pathway, and explain its putative mechanism. METHODS: A VR model was generated by ligation of coronary artery in mice. Two weeks after surgery, 60 mice were randomly divided into the model group, the sham-operation group (distilled water), the positive control group (2.4 mg/kg simvastatin), and the low-, medium- and high-dose LZD groups (2.1, 4.2, 8.4 g crude drug/kg, respectively) by a random number table, 10 mice in each group. Mice in each group was treated for 4 weeks. Changes of hemodynamics indices and cardiac weight index were detected by the PowerLab data acquisition and analysis recording instrument. Morphology changes of myocardial tissue were observed by hematoxylin-eosin and Masson staining. The expressions of TGF-ß1, Smad2, Smad3, p-Smad2 and p-Smad3 in myocardial tissue were detected by Western blotting. The mRNA expressions of TGF-ß1, Smad2 and Smad3 were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expressions of matrix metalloprotein 2 (MMP2), MMP9, collagen I and collagen III were observed by immunohistochemical methods. RESULTS: VR mice showed significant dysfunction in hemodynamic indices and cardiac structure and function. Compared with the shamoperation group, myocardial tissue damage, interstitial fibrosis occurred in the model mice, left ventricular systolic pressure (LVSP), left ventricular pressure maximum contraction rate (+dp/dtmax) and left ventricular pressure maximum relaxation rate (-dp/dtmax) decreased significantly (all P<0.01), while left ventricular end-diastolic pressure (LVEDP), cardiac weight index and left ventricular weight index elevated significantly, meanwhile TGF-ß1, p-Smad2, p-Smad3, Smad2, Smad3, MMP2, MMP9, collagen I, collagen III protein expressions in myocardial tissue and TGF-ß1, Smad2 and Smad3 mRNA expressions increased significantly (all P<0.01). Compared with the model group, LZD could signififi cantly improve the pathological changes of myocardial tissue, increase LVSP, +dp/dtmax and -dp/dtmax, lower LVEDP, reduce the whole heart weight index and left ventricular weight index and inhibit the over-expressions of TGF-ß1, p-Smad2, p-Smad3, Smad2, Smad3, MMP2, MMP9, collagen I and collagen III proteins in myocardial tissue and mRNA expressions of TGF-ß1, Smad2 and Smad3 (P<0.05 or P<0.01). CONCLUSION: LZD can significantly suppress VR induced by AMI, and its underlying mechanism may be associated with its inhibitory effect on the TGF-ß1/Smad signaling pathway.

3.
Nat Plants ; 4(2): 82-89, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29379155

RESUMO

Gnetophytes are an enigmatic gymnosperm lineage comprising three genera, Gnetum, Welwitschia and Ephedra, which are morphologically distinct from all other seed plants. Their distinctiveness has triggered much debate as to their origin, evolution and phylogenetic placement among seed plants. To increase our understanding of the evolution of gnetophytes, and their relation to other seed plants, we report here a high-quality draft genome sequence for Gnetum montanum, the first for any gnetophyte. By using a novel genome assembly strategy to deal with high levels of heterozygosity, we assembled >4 Gb of sequence encoding 27,491 protein-coding genes. Comparative analysis of the G. montanum genome with other gymnosperm genomes unveiled some remarkable and distinctive genomic features, such as a diverse assemblage of retrotransposons with evidence for elevated frequencies of elimination rather than accumulation, considerable differences in intron architecture, including both length distribution and proportions of (retro) transposon elements, and distinctive patterns of proliferation of functional protein domains. Furthermore, a few gene families showed Gnetum-specific copy number expansions (for example, cellulose synthase) or contractions (for example, Late Embryogenesis Abundant protein), which could be connected with Gnetum's distinctive morphological innovations associated with their adaptation to warm, mesic environments. Overall, the G. montanum genome enables a better resolution of ancestral genomic features within seed plants, and the identification of genomic characters that distinguish Gnetum from other gymnosperms.


Assuntos
Cycadopsida/genética , Evolução Molecular , Genoma de Planta/genética , Gnetum/genética , Cycadopsida/fisiologia , Variações do Número de Cópias de DNA , Elementos de DNA Transponíveis/genética , Desidratação , Duplicação Gênica , Genômica , Gnetum/fisiologia , Íntrons/genética , Anotação de Sequência Molecular , Folhas de Planta/genética , Folhas de Planta/fisiologia , Domínios Proteicos , Sequências Repetitivas de Ácido Nucleico/genética , Sementes/genética , Sementes/fisiologia
4.
Mol Med Rep ; 13(4): 3155-60, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26935156

RESUMO

The expression of microRNA (miR)-200b is suppressed in numerous tumor types, leading to epithelial-mesenchymal transition, which enables solid tissue epithelial cancers to invade and metastasize. The present study assessed the role of miR-200b in cervical cancer with the aim of clarifying the underlying pathophysiological mechanisms and to identify potential strategies for its prevention and treatment of cervical cancer. Reverse­transcription quantitative PCR revealed that miR­200b was downregulated in invasive cervical carcinoma tissues compared with that in normal adjacent tissues. A Transwell migration assay indicated that transfection of cervical cancer cells with miR­200b mimics significantly inhibited their migratory potential, while migration was enhanced in cells transfected with miR­200b inhibitor. Furthermore, western blot analysis indicated a negative correlation between miR­200b and mesenchymal marker vimentin as well as matrix metalloproteinase­9, which has a key role in tumor invasion and metastasis. In addition, a positive correlation between miR­200b and the epithelial marker E­cadherin was revealed by western blot and immunofluorescence. The results of the present study suggested that miR­200b suppressed the migratory potential of cervical carcinoma cells and therefore their ability to metastasize by inhibiting the epithelial-mesenchymal transition, which may be utilized for the treatment of cervical cancer.


Assuntos
Transição Epitelial-Mesenquimal , MicroRNAs/metabolismo , Neoplasias do Colo do Útero/patologia , Adulto , Idoso , Antígenos CD , Western Blotting , Caderinas/metabolismo , Movimento Celular , Regulação para Baixo , Feminino , Regulação Neoplásica da Expressão Gênica , Células HeLa , Humanos , Metaloproteinase 9 da Matriz/metabolismo , MicroRNAs/antagonistas & inibidores , MicroRNAs/genética , Microscopia de Fluorescência , Pessoa de Meia-Idade , Oligonucleotídeos Antissenso/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias do Colo do Útero/metabolismo , Vimentina/metabolismo
5.
Zhongguo Zhong Yao Za Zhi ; 40(7): 1388-91, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-26281566

RESUMO

OBJECTIVE: The study aimed to test if Paridis Rhizoma total saponins (PRTS) could induce apoptosis of human gastric cancer cell MKN-45. METHOD: Based on the previous researches, PRTS was set by different concentrations to treat human gastric cancer cell for 12 h (5, 10, 20 mg x L(-1)). Fluorescent staining methods were adopted to observe apoptotic morphological changes of MKN-45. The apoptosis rates were analyzed by flow cytometry with Annexin V-FITC/PI staining. The enzymatic activities of caspase-3 and caspase-8 were measured by ELISA. The protein levels of Fas and FasL were detected by Western blotting. RESULT: Under a fluorescence microscope, MKN-45 treated by PRTS was seen typical apoptotic morphological features. PRTS significantly increased the rate of apoptosis. Compared with the control group, there exsited significant differences in apoptosis rate of PRTS concentration of 20 mg x L(-1) (P < 0.01); besides, the enzymatic activities of caspase-3 and caspase-8 were promoted obviously after the effect of PRTS on MKN-45 cells for 12 h (P < 0.01). The protein levels of Fas and FasL in the MKN-45 were upgraded significantly. CONCLUSION: PRTS can induce apoptosis of human gastric cancer cell MKN-45 , which is concerned with caspase-3 and caspase-8 and upgraded Fas and FasL.


Assuntos
Apoptose/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Proteína Ligante Fas/metabolismo , Magnoliopsida/química , Saponinas/farmacologia , Neoplasias Gástricas/metabolismo , Receptor fas/metabolismo , Caspase 3/genética , Caspase 3/metabolismo , Caspase 8/genética , Caspase 8/metabolismo , Linhagem Celular Tumoral , Humanos , Rizoma/química , Transdução de Sinais/efeitos dos fármacos , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/genética , Neoplasias Gástricas/fisiopatologia
6.
Mol Phylogenet Evol ; 69(3): 1093-100, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23994356

RESUMO

To examine the phylogenetic relationships of Koenigia sensu lato (Polygonaceae), 43 samples representing all species of Koenigia and closely related taxa (e.g., Aconogonon, Bistorta, and Persicaria) were sequenced for nuclear ITS and four plastid regions (trnL-F, atpB-rbcL, rbcL, and rpl32-trnL((UAG))). Phylogenetic analyses indicate that Koenigia recognized by Hedberg is paraphyletic and that the basal species K. delicatula should be reassigned to a separate new genus. Based on these findings, we further propose that the genus Koenigia sensu lato be circumscribed to include five species. Ancestral state reconstruction showed that the pollen apertures likely evolved in parallel in the Aconogonon-Koenigia-Bistorta clade and Persicaria clade and that tricolpate pollen is most likely to be the ancestral state. Quincuncial aestivation likely evolved during the early evolution of Koenigia and its close relatives. Our findings suggest that the uplift of the Himalayas has played an important role in promoting species diversification of Koenigia. Koenigia islandica expanded its range during Pleistocene glacial cycles by tracking changes in newly available habitats.


Assuntos
Evolução Molecular , Filogenia , Polygonaceae/classificação , Teorema de Bayes , China , DNA de Cloroplastos/genética , DNA de Plantas/genética , DNA Espaçador Ribossômico/genética , Funções Verossimilhança , Modelos Genéticos , Pólen/anatomia & histologia , Polygonaceae/anatomia & histologia , Polygonaceae/genética , Análise de Sequência de DNA
7.
Zhonghua Nan Ke Xue ; 19(5): 446-50, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-23757970

RESUMO

OBJECTIVE: To study the effects of Wuzi Yanzong Pills (WYP) on sperm mitochondrial membrane potential (MMP) and its ultrastructure in oligo-asthenozoospermia model rats. METHODS: Oligo-asthenozoospermia models were made in 50 male rats weighing 200 - 220 g by intragastric administration of Tripterygium Glucosides at 30 mg per kg per d for 8 weeks, and then equally allocated to a model control, a Huangjing Zanyu Capsule (HZC) control, a low-dose WYP, a medium-dose WYP, and a high-dose WYP group. Another 10 age-matched normal male rats were included as normal controls. The rats in the model and normal control groups were given intragastrically distilled water at 10 ml/kg, those in the HZC group administered HZC at 3.01 g/kg, and those in the low-, medium- and high-dose WYP groups medicated with WYP at 2.30, 4.60 and 9.20 g/kg, respectively, once daily for 30 days. At 30 minutes after the last administration, we detected the sperm MMP by JC-1 fluorescent staining and flow cytometry, and examined the sperm ultrastructure under the JEM-1230 transmission electron microscope. RESULTS: JC-1 + % and its fluorescence intensity were (33.77 +/- 6.19)% and 1 468 +/- 496 in the model control, (56.34 +/- 10.35)% and 3 277 +/- 895 in the HZC control, (40.80 +/- 10.40)% and 2 016 +/- 767 in the low-dose WYP, (59.40 +/- 6.51)% and 3 897 +/- 643 in the medium-dose WYP, and (60.71 +/- 7.81)% and 3 371 +/- 647 in the high-dose WYP group, significantly reduced in comparison with (70.80 +/- 4.92)% and 4 360 +/- 945 in the normal control group (P < 0.05), but remarkably higher in the medium- and high-dose WYP groups than in the model controls (P < 0. 05). After modeling, the sperm membrane was loose and degenerated, the mitochondria swelling, variously sized and with incomplete membrane, and the axonemal structure unclear or ruptured. After 30 days of WYP administration, compared with the model control group, the rats exhibited integrated sperm membrane and mitochondrial membrane, reduced mitochondrial swelling and basically normal axonemal and microtubular structures. CONCLUSION: Tripterygium Glucosides could decrease the sperm mitochondrial membrane potential and damage the mitochondrial structure, while WYP could significantly increase the sperm mitochondrial membrane potential and reduce the sperm mitochondrial structure damage. The protection of the integrity of sperm mitochondrial structure and function is one of the mechanisms of WYP acting on oligo-asthenozoospermia.


Assuntos
Astenozoospermia , Medicamentos de Ervas Chinesas/farmacologia , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Oligospermia , Espermatozoides/efeitos dos fármacos , Animais , Astenozoospermia/patologia , Astenozoospermia/fisiopatologia , Masculino , Oligospermia/patologia , Oligospermia/fisiopatologia , Ratos , Ratos Sprague-Dawley , Espermatozoides/fisiologia , Espermatozoides/ultraestrutura
8.
Saudi Med J ; 33(5): 508-14, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22588811

RESUMO

OBJECTIVE: To investigate the mechanism of lipid raft mediating chemotherapy resistance in cervical cancer. METHODS: This experiment was carried out in the Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China from June 2010 to February 2011. Hela cells were divided into 6 groups: control group (Ctrl), cisplatin group (Cis), lipid raft interference agent group (MCD), NADPH oxidase inhibitor group (Apo), lipid raft interference agent combined with cisplatin group (MCD+Cis), and NADPH oxidase inhibitor combined with cisplatin group (Apo+Cis). After the cervical cancer cells were treated with a correspondent agent for 24 hours, the number of surviving cells were measured utilizing cell counting kits-8 (CCK-8), and the hypoxia inducible factor-1alpha (HIF-1alpha) levels were detected by Western blotting. Reactive oxygen species (ROS) levels were measured indirectly by detection of dichlorodihydrofluorescein fluorescence activity. RESULTS: The cell growth of MCD slowed down (survival cells was 62% compared with the Ctrl group), with the Apo group showing a similar effect (65% in the control group), and 49% for the Cis group, MCD+Cis was 21%, and Apo+Cis was 23%. While the level of HIF-1alpha protein and ROS of the MCD group, Apo group, Cis group, MCD+Cis group and Apo+Cis group were decreased significantly compared to the control group. The level of HIF-1alpha of MCD group decreased by 69.9%, Apo group by 60.2%, Cis group was 55.5%, MCD+Cis group by 21.1% and Apo+Cis group by 25.4%, while the level of ROS also decreased in the MCD group by 38.6%, Apo group by 35.3%, Cis group by 24%, MCD+Cis group by 12.3% and Apo+Cis group by 12.8%. CONCLUSION: Lipid raft may up-regulate ROS level and HIF-1alpha expression through activating NADPH oxidase, and thus promote chemotherapy resistance in cervical cancer.


Assuntos
Proliferação de Células/efeitos dos fármacos , Cisplatino/farmacologia , Resistencia a Medicamentos Antineoplásicos , NADPH Oxidases/antagonistas & inibidores , Acetofenonas/farmacologia , Ciclo Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Feminino , Células HeLa/efeitos dos fármacos , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Reguladores do Metabolismo de Lipídeos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Sensibilidade e Especificidade , Células Tumorais Cultivadas/efeitos dos fármacos , Neoplasias do Colo do Útero/tratamento farmacológico , Neoplasias do Colo do Útero/patologia
9.
Zhong Xi Yi Jie He Xue Bao ; 9(10): 1045-50, 2011 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-22015182

RESUMO

In this article, the authors firstly summarized the number of applications submitted to and projects supported by the National Natural Science Foundation of China (NSFC) in the field of traditional Chinese medicine research in 2010. Then they described the district distribution, research direction layout and allotment of the approved projects in the three primary disciplines (traditional Chinese medicine, Chinese materia medica and integrated traditional Chinese and Western medicine) and their 43 subdisciplines. The targeting suggestions for improvement were given respectively by concluding the reason of disapproved projects from the point of view of applicants and supporting institution, and by stating the common problems existing in the review process from the perspectives of fund managers and evaluation experts. Lastly, the major funding fields in the near future were predicted in the hope of providing guidance for applicants.


Assuntos
Fundações , Medicina Tradicional Chinesa , Apoio à Pesquisa como Assunto , China
10.
Yao Xue Xue Bao ; 45(12): 1545-9, 2010 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21351495

RESUMO

Our work focuses on the quality control and structural identification of Photocyanine as a cancer therapeutic photosensitizer. Photocyanine is a mixture which contains four ZnPcS2P2 type substituted Phthalocyanine isomers. In order to obtain the single component from Photocyanine, the mixture of four isomers possessing the similar structures and chemical property had been isolated and purified. An HPLC method with a mixture of methanol-acetonitrile-ion-pair buffer as the mobile phase was applied to isolate the four isomers by means of a semi-preparative C18 column. To remove the salts which were mixed in the preparative product, a SPE C18 column was used to separate the salts by elution with water and then the marker component was eluted by methanol. Subsequently, a column of Sephadex LH-20 gel was applied to elute the crudes with methanol to desalination. The purity of the isolated compound was measured by TLC and four different isomers of phthalocyanine were obtained. The chemical structures of them were elucidated by 1H NMR spectra, gCOSY and NOE1D. An HPLC-DAD method was developed for simultaneously determination of four major isomers in Photocyanine with a C18 column (Grace Smart, 150 mm x 4.6 mm ID, 5 microm). The separation was carried out with a gradient program at a flow rate of 1.0 mL x min(-1). The mobile phase was a mixture of acetonitrile and ion-pair buffer (0.01 mol x L(-1) hexadecyl trimethyl ammonium bromide and 0.01 mol x L(-1) potassium dihydrogen phosphate, adjusted the pH value to 6.8 with potassium hydroxide solution). The resolution values of four isomers were 2.5, 1.20, 1.33, and 1.8. Linear regression analysis for four compounds was performed by the external standard method. Four constituents were linear in the concentration range of 0.005 to 10 microg. The values of relative standard deviation (RSD) of intra-day were 0.12%, 0.66%, 0.99%, and 1.21%, respectively. The limits of detection for four compounds were 15 ng, 20 ng, 12 ng, and 25 ng, respectively. This method was simple, accurate and reproducible. The developed method can be successfully applied to analyze isomers in Photocyanine.


Assuntos
Antineoplásicos/química , Indóis/química , Compostos Organometálicos/química , Fármacos Fotossensibilizantes/química , Antineoplásicos/análise , Cromatografia Líquida de Alta Pressão/métodos , Indóis/análise , Isomerismo , Estrutura Molecular , Compostos Organometálicos/análise , Fotoquimioterapia , Fármacos Fotossensibilizantes/análise , Controle de Qualidade
11.
Guang Pu Xue Yu Guang Pu Fen Xi ; 29(5): 1354-7, 2009 May.
Artigo em Chinês | MEDLINE | ID: mdl-19650488

RESUMO

The UV-Visible absorption spectra and the fluorescence emission spectra of sixteen tetra-substituted metallo-phthalocyanine complexes {R4 PcM, where R = 2-[4-(2-sulfonic ethyl) piperazin-1-] ethoxyl (SPEO--), 2-(piperidin-1-yl) ethoxyl (PEO--); substitution position at alpha-position and beta-position of phthalocyanine ring; M = Zn(II), Ni(II), Co(II) and Cu(II)} were measured. The influence of different central ion, substituted group and its position, as well as different solvent on the Q-band of phthalocyanine complex in its UV-Vis absorption spectra was investigated. The influence of different central ion, substituted group and its position on the fluorescence emission spectra was discussed. Some properties of the UV-Vis absorption spectra such as the maximum absorption wavelength (lamdamax ) of Q-band and its molar extinction coefficient (epsilon), and those of the fluorescence emission spectra such as fluorescence quantum yield (phiF), fluorescence lifetime (r) and excited state energy (Es) were studied. The results showed that the lamdamax of Qband of all complexes were located at 681-718 nm, which had a distinct red shift in contrast with unsubstituted metallophthalocyanines (669-671 nm). All complexes of R4 PcM possessed a very high molar extinction coefficient up to 10(5) L x mol(-1) x cm(-1). And the UV-Vis absorption spectra and the fluorescence emission spectra of all complexes exhibited mirror shape concurrently. Two beta-substituted zinc phthalocyanine complexes with formula beta-(SPEO)4PcZn and beta-(PEO)4PcZn possessed very high molar extinction coefficient, fluorescence quantum yield and fluorescence lifetime specially. Therefore, it is hoped that these two would be developing to be new photosensitizers for photodynamic therapy (PDT)and photodynamic diagnosis (PDD).

12.
Anal Chem ; 81(11): 4476-81, 2009 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-19402619

RESUMO

In this paper, we report the IR (infrared) CVA (cyclic voltabsorptometry) and DCVA (derivative cyclic voltabsorptometry) spectroelectrochemical techniques to elucidate an electrochemical mechanism. First we set potassium ferrocyanide as an example to explain the validity of this method. Then the electrochemical redox of two compounds, 1,4-benzoquinone and 1,4-bis(2-ferrocenylvinyl)benzene, was selected to be examined with this method. 1,4-Benzoquinone exhibits two single-electron waves in the cyclic voltammetric (CV) experiment, whereas two electroactive groups (Fc) are contained in p-(Fc-CH=CH)(2)BZ, but only one redox wave is observed. IR CVA results show that three IR absorption peaks in 1,4-benzoquinone, 1232 cm(-1) (the absorption of final production), 1656 cm(-1) (the absorption of original reactant), and 1510 cm(-1) (the absorption of intermediate), and two IR absorption peaks in 1,4-bis(2-ferrocenylvinyl)benzene, 1620 cm(-1) (the absorption of final oxide production) and 1589 cm(-1) (the absorption of intermediate), can be used to track the electron transfer. On the basis of the IR absorbance at the appropriate monitored wavelength (mentioned above), we can analyze simultaneously the concentration change of the corresponding redox transition during CV scans. Also the combination of the DCVA spectroelectrochemical technique with theory analysis allows reconstructing the current-potential (i-E) curve for each step of electron transfer. The reconstructed i-E curve can help us to understand the electron-transfer process. We believe IR CVA and DCVA spectroelectrochemical techniques can be applicable to the study of a wide range of complex electrochemistry processes.

13.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(5): 953-6, 2007 May.
Artigo em Chinês | MEDLINE | ID: mdl-17655112

RESUMO

Two kinds of substituted phthalocyaninatozinc, tetra-beta-phthalimidobutoxy phthalocyaninatozinc (1) and tetra-beta-phthalimidomethyl phthalocyaninatozinc (2), were synthesized by mix melting method, which bear the same phthalimide substituents but the linking of the substituents to the phthalocyanine ring is different. The substituent linking to the phthalocyanine ring is one methylene in ZnPc (2), while the substituents linking to the phthalocyanine ring is four methylenes and one ather in ZnPc (1), in which ather is near by phthalocyanine ring. The absorption and fluorescence spectra and the abilities of singlet oxygen generation of these two phthalocyanines were compared with respect to the effect of different linked fashions. The magnitude of the redshift of absorption and fluorescence spectra relative to unsubstituded ZnPc is greater for tetra-beta-phthalimidobutoxy phthalocyaninatozinc (1) than for tetra-beta-phthalimidomethyl phthalocyaninatozinc (2). This result is related to the presence of stronger electron donating peripheral substituents of ZnPc (1), with phiF of (1) is larger than that of (2) and the abilities of singlet oxygen generation of (1) is smaller than that of (2).

14.
Pharmacol Biochem Behav ; 86(4): 741-8, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17448528

RESUMO

The present study aimed to determine whether Nicotiflorin, a natural flavonoid extracted from coronal of Carthamus tinctorius, has a protective effect on cerebral multi-infarct dementia in rats. The multi-infarct dementia model rats were prepared by injecting man-made micro-thrombi into the right hemisphere. The administration groups were treated once daily with 30, 60 and 120 mg/kg Nicotiflorin (i.g.) from 5 days before ischemia operation to 3 days after the operation for biochemical examination, 10 days for Morris water maze study and morphological observations and 20 days for eight-arm radial maze task. 2,3,5-triphenyltetrazolium chloride (TTC) staining showed that infarct volume of each Nicotiflorin administration group was much smaller than that of vehicle-treated multi-infarct dementia group, and hematoxylin and eosin (HE) staining showed that histopathological abnormalities of each Nicotiflorin group were also much lighter than that of vehicle-treated multi-infarct dementia group. Each Nicotiflorin group showed much better spatial memory performance in Morris water maze tests and eight-arm radial maze task compared with the vehicle-treated multi-infarct dementia group, significantly attenuated the elevation of lactic acid and malondialdehyde (MDA) contents and the decrease in lactate dehydrogenase (LDH), Na(+)K(+)ATPase, Ca(2+)Mg(2+)ATPase and superoxide dismutase (SOD) activity in the brain tissue which was composed of striatum, cortex and hippocampus of the ischemia hemisphere at day 3 after ischemia operation. These results suggest that Nicotiflorin has protective effects on reducing memory dysfunction, energy metabolism failure and oxidative stress in multi-infarct dementia model rats.


Assuntos
Demência por Múltiplos Infartos/tratamento farmacológico , Flavonoides/farmacologia , Transtornos da Memória/tratamento farmacológico , Fenóis/farmacologia , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Demência por Múltiplos Infartos/metabolismo , Demência por Múltiplos Infartos/patologia , Demência por Múltiplos Infartos/psicologia , Modelos Animais de Doenças , Metabolismo Energético/efeitos dos fármacos , Masculino , Aprendizagem em Labirinto/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley
15.
Acta Crystallogr Sect E Struct Rep Online ; 64(Pt 1): o324, 2007 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-21200885

RESUMO

In the molecule of the title compound, C(15)H(11)NO(2), the dihedral angle between the ring systems is 81.3 (2)°. In the crystal structure, mol-ecules are held together via C-H⋯O inter-actions.

16.
Guang Pu Xue Yu Guang Pu Fen Xi ; 27(11): 2329-32, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18260424

RESUMO

Tetrasulfophthalocyaninatozinc/titanium dioxide (ZnTsPc/TiO2) was synthesized by a novel in-situ and self-assembly process. The prepared products, including pure TiO2 and ZnTsPc, were characterized by XRD, UV-Vis, FTIR, Zeta potential and fluorescence spectroscopic techniques. The results indicated that ZnTsPc was indeed synthesized during the in-situ method and sol-gel process. A chemical bond (--SO2--O--Ti--) was also formed between the sensitizer (ZnTsPc) and the surface of titanium dioxide particles. The photocatalyst of ZnTsPc/TiO2 reveals greatly enhanced activity for the degradation of organic pollutant, Rhodamine B, under visible-light irradiation as compared with pure TiO2 and P25. The sensitization mechanism is that photo-generated charge carriers are separated by the chemical bond between ZnTsPc and TiO2.

17.
Acta Crystallogr C ; 62(Pt 9): i73-5, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16954608

RESUMO

The single-crystal X-ray diffraction structure analysis of an excellent non-linear optical material, viz. II-BaB2O4 or Ba3(B3O6)2, has been carried out at 163 and 293 K. The two sets of structural data are compared and indicate a significant shortening of the c axial length in the unit cell at 163 K, whereas the a and b axial lengths essentially do not change.

18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 26(3): 513-6, 2006 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-16830768

RESUMO

A dialkyloxy substituted metal-free phthalocyanine was synthesized by using 3-(2',2',4'-trimethyl-3'-pentoxy)-1,3-diiminoisoindoles and 1,3,3-trichloroisoindolenine as starting materials, and characterized by element analysis, 1H NMR, IR and UV/Vis spectra. The dependences of wavelength of UV/Vis spectra and its split of Q band absorption on the number of substituents and, dielectric constant of solvents are discussed based on the comparison with tetraalkyloxy substituted metal-free phthalocyanine.

19.
Guang Pu Xue Yu Guang Pu Fen Xi ; 26(12): 2280-2, 2006 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-17361730

RESUMO

The absorption spectral properties, fluorescence spectral properties and the abilities of photo-generating singlet oxygen of four phthalocyanines, namely tetra-alpha-(2,2,4-trimethyl-3-pentoxy) phthalocyaninatopalladium (zinc) and tetra-alpha-(2,4-diterbutylphenoxy) phthalocyaninatopalladium (zinc), were determined. Experimental results show that the ability of photo-generating singlet oxygen of phthalocyaninatopalladium is similar to that of phthalocyaninatozincx, though palladium(II) is an open shell electron structure and zinc( II ) is a closed shell structure. This may be concerned with a weak flourescence intensity and a strong inter-crossing transition of phthalocyaninatopalladium.

20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 26(11): 2050-2, 2006 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-17260754

RESUMO

UV-Vis absorption spectras of six series (18 kinds) of tetra- azo-aromaticoxy substituted metallophthalocyanines (R4 PcM, R = 4-pyridyloxy, 8-quinolinoxy, 2-methyl-8-quinolinoxy; substitution position: a position and beta position; M = Ni (II), Cu(II), Zn(II)) were measured. The effects of central mentals, the kinds and the positions of substitution groups, and solvents on the metallophthalocyanines' lamdamax in Q-band were discussed. Experimental data show: The lamdamax in Q-band of title complexes is about 680 nm. In contrast with substitution-free metallophthalocyanines(669-671 nm), the lamdamax in Q-band of the title complexes with the same central metal exhibits a different red-shift. The effect of substitution group's kinds on lamdamax in Q-band of the title complexes is more obvious in a position than in beta position, and with the same substitution group and central metal, lamdamax in Q-band of alpha position substituted complexes exhibits more obvious red-shift than beta position substituted complexes. The effects of central metal and solvent on lamda,ax in Q-band of the title complexes aren't obvious.

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