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Acta Biochim Biophys Sin (Shanghai) ; 36(4): 290-6, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15253155


The dependence of glycolate oxidase apoprotein (apoGO) activity on flavin analogs was surveyed in 9 higher plants from 7 families. Activities of all apoGOs depended not only on flavin mononucleotide (FMN) but also on flavin adenine dinucleotide (FAD), but not on riboflavin. The kinetic analysis showed that FMN was the optimum cofactor for apoGO from leaves of Brassica campestris. In plant kingdom, FMN, FAD and riboflavin are three flavin analogs with very similar structure, and they could coexist and be inter-converted from each other, so the question is how the apoprotein of glycolate oxidase (GO) recognized these flavin analogs. No inhibition effect of riboflavin on the activity of apoGO with FMN or FAD was found and no obvious quenching of riboflavin or apoGO protein fluorescence was detected with the addition of apoGO or riboflavin, respectively. These results indicated that riboflavin did not bind to apoGO tightly like FMN and FAD. Inorganic phosphate (Pi) did inhibit the activity of GO, and kinetic analysis revealed that this inhibition was caused by the competitive binding to apoGO between Pi and FMN. This competitive binding was further confirmed by the inhibition of Pi to the quenching of FMN and apoGO protein fluorescence with apoGO and FMN, respectively. It was suggested that the 5'-phosphate group of FMN or FAD may play a key role in the recognition and binding of riboflavin analog cofactors with apoGO.

Oxirredutases do Álcool/química , Apoproteínas/química , Mononucleotídeo de Flavina/metabolismo , Flavinas/química , Plantas/enzimologia , Oxirredutases do Álcool/isolamento & purificação , Oxirredutases do Álcool/metabolismo , Apoproteínas/genética , Apoproteínas/isolamento & purificação , Apoproteínas/metabolismo , Ligação Competitiva , Brassica/enzimologia , Mononucleotídeo de Flavina/química , Flavina-Adenina Dinucleotídeo/química , Flavina-Adenina Dinucleotídeo/metabolismo , Cinética , Fosfatos/metabolismo , Folhas de Planta/química , Sensibilidade e Especificidade , Espectrometria de Fluorescência