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1.
Biomed Res Int ; 2020: 3981931, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32090086

RESUMO

Various microRNAs (miRNAs) are of importance in the development of colon cancer, but most of the mechanisms of the miRNAs are still unclear. In order to clarify the hub miRNAs and their roles in colon cancer development, GSE98406 was used to screen hub miRNAs by bioinformatics analysis. 46 DE-miRNAs (14 were upregulated and 32 were downregulated) and 1738 target genes of DE-miRNAs were ascertained. miRNAs-gene-networks and miRNAs-GO-networks were built to get more knowledge about the function of candidate miRNAs. After validation, three miRNAs (miR-17-5p, miR-182-5p and miR-200a-3p) were recognized to be hub miRNAs associated with the progression of colon cancer. More importantly, the hub miRNAs and the putative targets genes might be new diagnostic and therapeutic targets for colon cancer in the future.

2.
Discov Med ; 27(150): 227-233, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31421691

RESUMO

OBJECTIVE: The aim of this study was to investigate the characteristics of nasopharyngeal carcinoma (NPC) using contrast-enhanced ultrasonography (CEUS), including the enhancement patterns and the quantitative parameters. METHODS: Having been scanned using conventional ultrasonography (US) and CEUS, every case was confirmed to be NPC under endoscopic biopsy, and no case received any anti-tumor treatment before CEUS examinations. Tumor/node/metastasis stages were determined in accordance with 2002 AJCC 6th edition. Contrast enhancement patterns and quantitative parameters were observed. RESULTS: CEUS imaging of NPC showed that the tumor signal intensity enhanced early, rapidly, and remarkably, and decreased slowly later. The patterns of enhancement included spot/linear enhancement, peripheral enhancement, and mass enhancement, and two types of time intensity curves of NPC included type I and type II. There was a significant difference between peak intensity (PI) and T stage (P<0.05), whereas time-to-peak (TP) and slope did not show significant differences with T stage (P>0.05). CONCLUSION: CEUS is feasible to be applied to the nasopharynx region. The use of CEUS makes it possible to observe vascular permeability of NPC. Our results suggest that the quantitative parameter PI of nasopharyngeal carcinoma is significantly different from T stages. Thus, PI may serve as a potential noninvasive radiological prognostic indicator for NPC.


Assuntos
Meios de Contraste/química , Aumento da Imagem , Carcinoma Nasofaríngeo/diagnóstico por imagem , Ultrassonografia , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Carcinoma Nasofaríngeo/patologia , Estadiamento de Neoplasias
3.
J Cancer Res Clin Oncol ; 145(5): 1201-1211, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30863898

RESUMO

BACKGROUND: Dose-response meta-analysis (DRMA) has been widely used in exploring cancer risk factors. Understanding the quality of published DRMAs on cancer risk factors may be beneficial for informed prevention for cancer. METHODS: We searched eligible DRMAs from 1st January 2011 to 31st-July-2017. The modified AMSTAR 1.0 (15 items) and PRISMA checklist (26 items) were used to evaluate the methodological and reporting quality of included DRMAs. We compared the adherence rate of these items by journal type, publication years, region, and funding information, in prior. RESULTS: We included 260 DRMAs. Colorectal, breast, prostate, and lung were the four most commonly investigated cancers. For methodological quality, 6 out of 15 items were adhered by less than 30% of the DRMAs, 2 by less than 60%, only 7 of which by 80% or more. For reporting quality, 3 out of 26 items were adhered by less than 30% of the DRMAs, 1 by less than 80% (> 30%), and 20 of which by 80% or more. Those published in general journal, published more recently, and received any financial support have better methodological (Rate differences, RDs = 10-36%; P < 0.05) and reporting adherence (RDs = 12-36%; P < 0.05). DRMAs by Asian author tend to be less qualified than by European and American. CONCLUSIONS: The methodological quality of DRMAs on cancer risk factors is worrisome that the findings of them may be deflective; more efforts are needed to improve the validity of it.


Assuntos
Neoplasias/epidemiologia , Neoplasias/prevenção & controle , Pré-Medicação , Humanos , Vigilância da População , Pré-Medicação/métodos , Pré-Medicação/normas , Relatório de Pesquisa/normas , Cooperação e Adesão ao Tratamento
4.
J Biomed Opt ; 22(3): 36008, 2017 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-28271123

RESUMO

Harmonic generation microscopy (HGM) has become one unique tool of optical virtual biopsy for the diagnosis of cancer and the in vivo cytometry of leukocytes. Without labeling, HGM can reveal the submicron features of tissues and cells in vivo. For deep imaging depth and minimal invasiveness, people commonly adopt 1100- to 1300-nm femtosecond laser sources. However, those lasers are typically based on bulky oscillators whose performances are sensitive to environmental conditions. We demonstrate a fiber-based 1150-nm femtosecond laser source, with 6.5-nJ pulse energy, 86-fs pulse width, and 11.25-MHz pulse repetition rate. It was obtained by a bismuth borate or magnesium-doped periodically poled lithium niobate (MgO:PPLN) mediated frequency doubling of the 2300-nm solitons, generated from an excitation of 1550-nm femtosecond pulses on a large mode area photonic crystal fiber. Combined with a home-built laser scanned microscope and a tailor-made frame grabber, we achieve a pulse-per-pixel HGM imaging in vivo at a 30-Hz frame rate. This integrated solution has the potential to be developed as a stable HGM system for routine clinical use.


Assuntos
Diagnóstico por Imagem/instrumentação , Lasers , Microscopia/instrumentação , Luz , Fótons
5.
Sci Rep ; 7: 40554, 2017 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-28091563

RESUMO

Although the association between CAG and GGN repeats in the androgen receptor gene and prostate cancer risk has been widely studied, it remains controversial from previous meta-analyses and narrative reviews. Therefore, we performed this meta-analysis to provide more precise estimates with sufficient power. A total of 51 publications with 61 studies for CAG repeats and 14 publications with 16 studies for GGN repeats were identified in the meta-analysis. The results showed that short CAG repeats (<22 repeats) carriers presented an elevated risk of prostate cancer than long CAG repeats (≥22) carriers (OR = 1.31, 95% CI 1.16 to 1.47). Prostate cancer cases presented an average fewer CAG repeats (MD = -0.85, 95% CI -1.28 to -0.42) than controls. Short GGN repeats (≤16) carriers presented an increased risk of prostate cancer than long GGN repeats (>16) carriers (OR = 1.38, 95% CI 1.05 to 1.82). In subgroup analyses, the abovementioned significant association was predominantly observed in Caucasian populations. The meta-analysis showed that short CAG and GGN repeats in androgen receptor gene were associated with increased risk of prostate cancer, especially in Caucasians.


Assuntos
Predisposição Genética para Doença , Polimorfismo Genético , Neoplasias da Próstata/genética , Receptores Androgênicos/genética , Estudos de Casos e Controles , Estudos de Associação Genética , Haplótipos/genética , Humanos , Masculino , Viés de Publicação , Fatores de Risco , Tamanho da Amostra , Repetições de Trinucleotídeos/genética
6.
Medicine (Baltimore) ; 95(21): e2302, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27227908

RESUMO

There are inconsistent data on the association of risk of hepatitis virus infection and hepatitis virus-related diseases with the toll-like receptor 3 (TLR3) gene.Several common polymorphism sites were targeted to assess the risk of HBV infection, HCV infection, and HBV-related diseases.Meta-analysis combining data for 3547 cases and 2797 controls from 8 studies was performed in this study. Pooled ORs were calculated to measure the risk of hepatitis virus infection and hepatitis virus-related diseases. Fixed-effects pooled ORs were calculated using the Mantel-Haenszel method.The TLR3 gene was associated with a significantly increased risk of HBV-related diseases among 1355 patients and 1130 controls ([pooled OR, [95%CI]: 1.30, [1.15-1.48] for dominant; 1.77, [1.35-2.31] for recessive; 1.28 [1.16-1.41] for allele frequency). Subgroup analyses by a polymorphism site indicated an increased risk of HCV infection in relation to the TT/CT genotypes of rs3775291 (1.50 [1.11-2.01]), and a decreased risk ascribed to the T allele (0.20 [0.16-0.25]). We also noted an association between rs3775291 and significantly increased risk of HBV-related diseases (2.23 [1.55-3.21]). No significant inter-study heterogeneity or publication bias was detected in the analyses.These data suggest a likely effect on the risk to infect HCV and develop HBV-related diseases for the TLR3 gene. Large-scale studies with racially diverse populations are required to validate these findings.


Assuntos
Hepatite B/genética , Hepatite C/genética , Receptor 3 Toll-Like/genética , Alelos , Frequência do Gene , Genótipo , Humanos , Polimorfismo de Nucleotídeo Único
7.
Onco Targets Ther ; 9: 2403-8, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27143935

RESUMO

PURPOSE: Numerous studies have suggested that the interleukin-4 (IL-4) rs2243250 polymorphism is associated with gastric cancer susceptibility. However, the results were inconsistent. Hence, we carried out a meta-analysis to confirm the conclusion. METHODS: We searched PubMed, Embase, CBM, CNKI, and Wanfang Data to identify relevant studies up to August 20, 2015. Odds ratio (OR) and 95% confidence interval (CI) were used to estimate the association between IL-4 rs2243250 polymorphism and gastric cancer susceptibility. All the statistical analyses were performed with Stata 12.0 software. RESULTS: A total of eleven published case-control studies were identified, including 2,247 gastric cancer patients and 3,370 controls. Overall, no significant association between IL-4 rs2243250 polymorphism and gastric cancer susceptibility was observed in this meta-analysis (T vs C: OR =1.05, 95% CI =0.95-1.17; TT vs CC: OR =1.20, 95% CI =0.89-1.63; CT vs CC: OR =1.14, 95% CI =0.87-1.48; TT + CT vs CC: OR =1.13, 95% CI =0.89-1.44; TT vs CT + CC: OR =1.02, 95% CI =0.88-1.20). Similar results were found in subgroup analyses according to ethnicity and Hardy-Weinberg equilibrium in controls. CONCLUSION: This meta-analysis suggests that IL-4 rs2243250 polymorphism may not be associated with gastric cancer susceptibility. Further studies are needed to validate this conclusion.

8.
Biomed Rep ; 4(5): 595-600, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27123253

RESUMO

A number of studies have suggested that the Mycobacterium vaccae (MV) vaccine as an adjunctive therapy has a positive effect in the treatment of multidrug-resistant tuberculosis (MDR-TB). However, the result is inconclusive. The aim of the present study was to systematically evaluate the effect and safety of MV as an adjunctive therapy in the treatment of MDR-TB. A computerized search of PubMed, Embase, Cochrane Central Register of Controlled Trials, CBM, CNKI and VIP until October 2014 was conducted to collect the relevant studies. The main outcome measures were the sputum smear positive-turned-negative rate, the absorption rate of TB foci and the closure situation of the TB cavity. Two investigators identified the eligible studies and extracted data independently. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated and pooled using the fixed effects model. A total of 25 studies involving 2,281 patients with MDR-TB were included. The pooled OR was 3.84 (95% CI, 3.84-4.73) for the sputum smear positive-turned-negative, 4.08 (95% CI, 3.08-5.45) for the absorption rate of TB foci, and 3.42 (95% CI, 2.68-4.37) for the closure situation of TB cavity. Therefore, MV has a significant effect as an adjunctive therapy in the treatment of MDR-TB. However, larger scale multicenter randomized controlled trials are required to confirm this evidence for limited latent bias at present.

9.
J Endourol ; 29(8): 925-8, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25556314

RESUMO

PURPOSE: This study aimed to evaluate the efficacy and safety of transurethral plasmakinetic resection of the prostate (PKRP) for the treatment of patients with benign prostatic hyperplasia (BPH) in men with prostate volume >80 cc. PATIENTS AND METHODS: From January 2010 to December 2011, 120 patients were included in our study; among these patients, 66 had prostate sizes of 80 cc to 100 cc and 54 had prostate sizes >100 cc. Pre-, peri- and postoperative evaluations were performed. RESULTS: The mean operative duration of PKRP was 112 minutes, and the mean resected tissue weight was 60.01 g. Hemoglobin level decreased by 0.65±0.52 g/dL, and serum sodium content decreased by 0.06±2.62 mmol/L. The mean catheterization time was 83.05 hours, and the mean hospital stay was 12.47 days. The short follow-up time (30 months) demonstrated significant improvement in International Prostate Symptom Score, quality of life, and postvoid residual volume compared with preoperative characteristics. One patient underwent reoperation because of a blood clot in the bladder. Twelve patients complained of retrograde ejaculation. No patient experienced urinary incontinence. CONCLUSIONS: PKRP is safe and efficacious for men with BPH who have a large prostate (volume >80 cc).


Assuntos
Eletrocirurgia/métodos , Hiperplasia Prostática/cirurgia , Ressecção Transuretral da Próstata/métodos , Idoso , Idoso de 80 Anos ou mais , Eletrocirurgia/efeitos adversos , Feminino , Seguimentos , Humanos , Tempo de Internação/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Duração da Cirurgia , Tamanho do Órgão , Complicações Pós-Operatórias/etiologia , Próstata/patologia , Hiperplasia Prostática/patologia , Qualidade de Vida , Estudos Retrospectivos
10.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 22(5): 1273-7, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25338571

RESUMO

This study was purposed to find new biomarkers and to establish protein finger print model for diagnosis of leukemia. A total of 40 leukemia samples and 37 healthy control samptes were tested by surface enhance laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF- MS). The data of spectra were analyzed by bioinformatics tools like Biomarker Patterns 5.0 and discriminant analysis to establish diagnostic mode1. The results showed that 22 protein features were stably detected by protein fingerprint, The detective model combined with 3 biomarkers (m/z 4650, 8609 and 11660) could differentiate leukemia with sensitivity of 97.5% (39/40) and specificity of 91.9%(34/37). It is concluded that the detective model established by 3 protein features may be a novel method for diagnosis of leukemia.


Assuntos
Biomarcadores Tumorais/análise , Leucemia/diagnóstico , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Humanos , Peso Molecular , Mapeamento de Peptídeos
11.
Mol Biol Rep ; 41(10): 6713-7, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24990700

RESUMO

The association between vitamin D receptor (VDR) gene polymorphisms and risk of benign prostatic hyperplasia (BPH) has been investigated in numerous publications, but published results remain inconclusive. Hence, we conducted this meta-analysis to derive a more precise conclusion. Four polymorphisms (Taq-I, Bsm-I, Apa-I, and Fok-I) of the VDR gene with risk of BPH from six case-control studies and one cohort study involving 2,248 individuals were identified from PubMed and China National Knowledge Internet databases up to November 20, 2013 (updated on March 5, 2014). After data extraction, the meta-analysis was performed using Comprehensive Meta-Analysis software. All four VDR polymorphisms were not associated with the risk of BPH [Taq-I: OR 0.61, 95 % CI (0.38-1.24) for tt vs. TT; Bsm-I: OR 1.27, 95 % CI (0.96-1.69) for bb vs. BB; Apa-I: OR 1.26, 95 % CI (0.64-2.46) for aa vs. AA; Fok-I: OR 0.95, 95 % CI (0.60-1.50) for ff vs. FF]. Subgroup analysis according to ethnicity for Taq-I polymorphism also showed that the polymorphism was not associated with risk of BPH for either Caucasians [OR 0.74, 95 % CI (0.31-1.78) for tt vs. TT] or Asians [OR 0.35, 95 % CI (0.11-1.11) for tt vs. TT]. However, results of this meta-analysis should be treated with caution because this meta-analysis has several limitations. We propose to conduct a high-quality study with large sample size to further identify the association between VDR gene polymorphisms and BPH susceptibility.


Assuntos
Polimorfismo Genético , Hiperplasia Prostática/genética , Receptores de Calcitriol/genética , Estudos de Casos e Controles , Predisposição Genética para Doença , Humanos , Masculino , Razão de Chances , Polimorfismo de Fragmento de Restrição , Viés de Publicação , Risco
12.
J Endourol ; 28(8): 946-50, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24708300

RESUMO

PURPOSE: To evaluate the efficacy and safety of retroperitoneal laparoscopic pyelolithotomy (RLP) versus percutaneous nephrolithotomy (PCNL) for large renal pelvic calculi using a randomized controlled trial. MATERIALS AND METHODS: Patients with large renal pelvic calculi were prospectively randomized using matched-pair analysis (1:1 scenario) into either the RLP group or the PCNL group. The patients in each group underwent the procedure accordingly. Treatment efficacy, safety, and complications were evaluated after surgery. RESULTS: Finally, 178 eligible patients were included and the demographics and mean stone size of two groups were similar. We found no significant differences in the mean postoperative hospital stay (4.5±2.3 vs. 4.3±1.3 days), rate of blood transfusion (0% vs. 1.1%), conversion rate (0% vs. 3.4%), and rate of total postoperative complication (p>0.05). The procedural duration and mean drop in hemoglobin levels were significantly lower in the RLP group as compared with the PCNL group (90.87±33.4 vs. 116.8±44.4 minutes, p<0.001; 0.9±0.5 vs. 1.7±1.3 g/dL, p<0.001, respectively). Significant differences were also observed in the stone-free rate (98% vs. 90%, p=0.03) and postoperative fever rate (3.4% vs. 13.5%, p=0.02). CONCLUSIONS: Current evidence suggests that PCNL and RLP are both effective and safe for the treatment of large renal pelvic calculi. Our study shows that, compared with the PCNL approach, RLP for large renal pelvic stone resulted in shorter operative time, less bleeding, less postoperative fever, and a higher stone-free rate. Data from larger, multicenter randomized controlled trials of high quality are needed to further confirm our findings.


Assuntos
Cálculos Renais/cirurgia , Pelve Renal/cirurgia , Laparoscopia/métodos , Nefrostomia Percutânea/métodos , Adulto , Idoso , Transfusão de Sangue/estatística & dados numéricos , China , Feminino , Humanos , Laparoscopia/efeitos adversos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Nefrostomia Percutânea/efeitos adversos , Duração da Cirurgia , Projetos Piloto , Estudos Prospectivos , Espaço Retroperitoneal , Resultado do Tratamento
13.
Zhong Xi Yi Jie He Xue Bao ; 7(11): 1047-51, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-19912736

RESUMO

BACKGROUND: In recent years, Shenyi Capsule has been proven to have certain anti-angiogenic effects, and to be effective to many cancers, but its effects on advanced esophageal cancer are scarcely studied. OBJECTIVE: To observe the effects of Shenyi Capsule combined with gemcitabine plus cisplatin (GP) regimen in treatment of advanced esophageal cancer. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS: Sixty inpatients with advanced esophageal cancer from Henan Tumor Hospital, and the Fist Affiliated Hospital of Zhengzhou University were included and randomly divided into treatment group and control group. There were 30 cases in each group. Patients in the treatment group were treated with Shenyi Capsule combined with GP regimen, and patients in the control group were treated with GP regimen alone. MAIN OUTCOME MEASURES: The total response rate was calculated. The level of vascular endothelial growth factor (VEGF), the chemotherapy side reaction and quality of life in the two groups were evaluated. The follow-up of survival time was conducted too. RESULTS: There was no significant difference in total response rate between the two groups (P=0.264). The levels of VEGF in the two groups were decreased as compared with that before the treatment. After treatment, the VEGF level in the treatment group was lower than that in the control group (P=0.002). The decline rates of white blood cell and blood platelet, and the incidence rate of nausea and vomiting in the treatment group were lower than those in the control group, and there were significant differences between the two groups (P=0.045, P=0.036, P=0.037). The quality of life of the patients in the treatment group was better than that in the control group (P=0.028), and one-year survival rate in the treatment group was higher than that in the control group (P=0.047). CONCLUSION: Shenyi Capsule combined with GP regimen is feasible and safe in treatment of advanced esophageal cancer, and the effects are better than chemotherapy alone. It can improve the total response rate, and is effective in inhibiting new angiogenesis of esophageal cancer, reducing chemotherapy side reaction, and improving the patients' quality of life and survival rates.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Cisplatino/administração & dosagem , Desoxicitidina/análogos & derivados , Neoplasias Esofágicas/tratamento farmacológico , Ginsenosídeos/uso terapêutico , Idoso , Cápsulas , Cisplatino/efeitos adversos , Desoxicitidina/administração & dosagem , Desoxicitidina/efeitos adversos , Feminino , Ginsenosídeos/efeitos adversos , Humanos , Masculino , Pessoa de Meia-Idade , Panax/química , Fitoterapia , Qualidade de Vida , Taxa de Sobrevida , Fator A de Crescimento do Endotélio Vascular/análise
14.
Huan Jing Ke Xue ; 29(5): 1380-7, 2008 May.
Artigo em Chinês | MEDLINE | ID: mdl-18624211

RESUMO

The changes in microbial biomass carbon (MBC), microbial biomass nitrogen (MBN), dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) were examined in order to assess the effect of surface layer soil (0 - 10 cm) under different land-use types after freshwater marshes tillage in the Sanjiang Plain Northeast China. Land uses were Deyeuxia angustifolia freshwater marshes ((DAM), cultivated land (CL), recovery freshwater marsh (RFM), constructed woodland (CW). After DAM soil tillage, MBC, MBN, DOC and DON declined strongly in agricultural surface soil layer, decreased 63.8%-80.5% (MBC), 56.3%-67.1% (MBN), 43.1%-44.3% (DOC) and 25.2%-56.1% (DON) respectively. In contrast, these C, N fraction had significant recovered in RFM and CW surface soil, increased 36.1%-59.9% (MBC), 46.7%-65.9% (MBN), 67.0%-69.3% (DOC)and 81.2%-88.3% (DON) respectively. Cultivation and land-use affected soil MBC, MBN, DOC and DON intensely. Therefore these labile C, N fractions have the significant relative under different land-use types. However DOC was more obvious controlled than DON by the land-use types. The relative between DOC and MBC, MBN have much difference than DON, the main reason of this distinction is the diverse source in available carbon and nitrogen that taken by microbial property under different land uses.


Assuntos
Carbono/análise , Água Doce/análise , Nitrogênio/análise , Microbiologia do Solo , Áreas Alagadas , Biomassa , Ecossistema , Água Doce/microbiologia , Poaceae/crescimento & desenvolvimento , Solo/análise , Soja/crescimento & desenvolvimento , Árvores/crescimento & desenvolvimento
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