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1.
World Neurosurg ; 133: e31-e61, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31415895

RESUMO

BACKGROUND: The association between matrix metalloproteinase 9 (MMP-9) gene -1562C/T (rs3918242) polymorphism and the susceptibility of ischemic stroke (IS) has been investigated. However, results were ambiguous and inconsistent. Therefore, we performed this study to better assess the potential relationship between rs3918242 polymorphism and susceptibility risk of IS. METHODS: We included case-control studies concerning the relationship between the rs3918242 polymorphism and IS, and odds ratios with corresponding 95% confidence intervals were used to describe the associations. Furthermore, meta-regression analyses, heterogeneity, cumulative analyses, sensitivity analyses, and publication bias were examined. RESULTS: A total of 19 studies were included for analysis. Significant associations with the risk of IS were detected for the rs3918242 polymorphism in overall population, Asians, and whites. When available data were stratified by gender, we found a significant correlation with the risk of IS in both males and females. Further subgroup analysis by the subtypes of IS showed that the rs3918242 polymorphism was significantly correlated with the risk of patients with large artery atherosclerosis. When stratified by age, we found that the rs3918242 polymorphism was significantly correlated with the risk of IS in patients both aged ≥65 years and >65 years. Both the diabetes and the nondiabetes subgroups reached significant results, and in an analysis stratified by smoking status, an increased risk of IS was associated with smoking. CONCLUSIONS: The rs3918242 polymorphism may be a susceptible predictor of susceptibility of IS. Further large-scale studies are needed to verify the results of our findings.


Assuntos
Isquemia Encefálica/genética , Metaloproteinase 9 da Matriz/genética , Polimorfismo de Nucleotídeo Único , Adulto , Distribuição por Idade , Idoso , Aterosclerose/epidemiologia , Isquemia Encefálica/enzimologia , Isquemia Encefálica/epidemiologia , Estudos de Casos e Controles , Fumar Cigarros/epidemiologia , Comorbidade , Intervalos de Confiança , Diabetes Mellitus/epidemiologia , Grupos Étnicos , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Embolia Intracraniana/epidemiologia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Distribuição por Sexo
2.
Neuromolecular Med ; 20(2): 271-279, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29713907

RESUMO

A quantitative transcriptomics analysis has reported that Calmodulin 1 (CALM1) is highly expressed in human brain tissues. This study aims to evaluate the relationship between CALM1 rs3179089 polymorphism and ischemic stroke (IS) in Chinese Han population. A total of 550 patients with IS and 550 control subjects were recruited and genotyped using Sequenom MassArray technology. The mRNA expression of CALM1 was measured using quantitative real-time polymerase chain reaction. CALM1 mRNA expression was significantly higher in patients with IS than that in control subjects (P = 0.006). The genomic frequency distribution was significantly different between female patients with IS and female controls (χ2 = 6.043, P = 0.047). In recessive model, CALM1 rs3179089 polymorphism was associated with the risk of IS in female patients. GG genotype significantly increased the risk of IS compared with the CC+GC genotype in females (OR 8.68, P = 0.042; adjusted OR 8.72, Padj = 0.042). Rs3179089 polymorphism was associated positively with plasmas D-Dimer of patients with IS in recessive model (ßa = 3.24, P = 0.018; ßb = 3.20, Padj = 0.019). Moreover, rs3179089 polymorphism was related positively to thrombin time of patients with IS in addictive (ßa = 2.32, P = 0.005, ßb = 2.26, Padj=0.006) and recessive model (ßa = 11.19, P = 0.001, ßb = 11.13, Padj = 0.001). CALM1 expression was involved in the development of IS. CALM1 rs3179089 polymorphism was associated with IS risk in Chinese females, and related to blood coagulation of IS patients.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Isquemia Encefálica/genética , Calmodulina/genética , Grupos Étnicos/genética , Proteínas do Tecido Nervoso/genética , Polimorfismo de Nucleotídeo Único , Idoso , Glicemia/análise , Química Encefálica , Isquemia Encefálica/etnologia , Calmodulina/biossíntese , Calmodulina/fisiologia , Estudos de Casos e Controles , Feminino , Produtos de Degradação da Fibrina e do Fibrinogênio/análise , Genes Recessivos , Predisposição Genética para Doença , Humanos , Lipídeos/sangue , Masculino , Modelos Genéticos , Proteínas do Tecido Nervoso/biossíntese , Proteínas do Tecido Nervoso/fisiologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Fatores de Risco , Caracteres Sexuais , Tempo de Trombina
3.
Neurol Sci ; 39(1): 127-133, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29075930

RESUMO

This study aimed to evaluate the association of the toll-like receptor 4 (TLR4) polymorphisms rs1927914, rs10759932, and rs11536889 with susceptibility to ischemic stroke (IS) and the serum levels of inflammatory cytokines. A total of 816 IS patients and 816 control subjects were genotyped using Sequenom MassARRAY technology. The serum levels of interleukin 1 beta (IL-1ß), interleukin 6 (IL-6), interleukin 8 (IL-8), and tumor necrosis factor alpha (TNFα) were measured by enzyme-linked immunosorbent assay. rs1927914 was significantly associated with male IS patients in the additive model [odds ratio (OR) = 0.81; 95% confidence interval (CI) = 0.67-0.99; P = 0.039] and in the allele model (OR = 0.81; 95% CI = 0.66-0.99; P = 0.037). In the dominant model, rs10759932 was significantly associated with the serum TNFα level of the male IS patients [regression coefficient (ß) = 0.15; 95% CI = 0.01-0.29; P adj = 0.042]. This polymorphism was also correlated with the serum IL-8 level of female IS patients in the additive model (ß = 0.24; 95% CI = 0.25-0.43; P adj = 0.021) and in the recessive model (ß = 0.65; 95% CI = 0.11-1.11; P adj = 0.026). The TLR4 gene rs1927914 polymorphism was associated with susceptibility to IS in males. Moreover, the rs10759932 polymorphism may affect inflammatory response in IS patients.


Assuntos
Isquemia Encefálica/genética , Inflamação/genética , Acidente Vascular Cerebral/genética , Receptor 4 Toll-Like/genética , Idoso , Grupo com Ancestrais do Continente Asiático , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Predisposição Genética para Doença , Genótipo , Humanos , Inflamação/sangue , Mediadores da Inflamação/sangue , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único
4.
Nanomedicine ; 13(6): 1913-1923, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28400159

RESUMO

Surface modification on titanium implants plays an important role in promoting mesenchymal stem cell (MSC) response to enhance osseointegration persistently. In this study, nano-scale TiO2 nanotube topography (TNT), micro-scale sand blasted-acid etched topography (SLA), and hybrid sand blasted-acid etched/nanotube topography (SLA/TNT) were fabricated on the surfaces of titanium implants. Although the initial cell adherence at 60 min among TNT, SLA and TNT/SLA was not different, SLA and SLA/TNT presented to be rougher and suppressed the proliferation of MSC. TNT showed hydrophilic surface and balanced promotion of cellular functions. After being implanted in rabbit femur models, TNT displayed the best osteogenesis inducing ability as well as strong bonding strength to the substrate. These results indicate that nano-scale TNT provides favorable surface topography for improving the clinical performance of endosseous implants compared with micro and hybrid micro/nano surfaces, suggesting a promising and reliable surface modification strategy of titanium implants for clinical application.


Assuntos
Nanotubos/química , Osteogênese/efeitos dos fármacos , Próteses e Implantes , Titânio/farmacologia , Topografia Médica/métodos , Animais , Interações Hidrofóbicas e Hidrofílicas , Masculino , Células-Tronco Mesenquimais/efeitos dos fármacos , Osseointegração/efeitos dos fármacos , Coelhos , Ratos , Ratos Sprague-Dawley , Propriedades de Superfície , Titânio/química
5.
Neurol Sci ; 37(9): 1537-44, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27262705

RESUMO

Many studies reported that toll-like receptors (TLRs) played an important role in the process of ischemic stroke (IS). However, the impact of TLR5 rs5744174 on stroke risk, gene expression and on inflammatory cytokines, and lipid levels in ischemic stroke patients has not yet been reported and was therefore the subject of this study. In this case-control study, a total of 816 ischemic stroke patients and 816 healthy controls were genotyped using Sequenom MassArray technology. The mRNA expression of TLR5 was detected through quantitative real-time PCR among 52 ischemic stroke patients. The levels of IL-1b, IL-6, IL-8, and TNFα were measured by ELISA among 62 IS patients. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined among 816 IS patients using a Hitachi 7600 Automatic Biochemistry Analyzer. Our result showed TLR5 rs5744174 polymorphism was not associated with stroke risk, TLR5 mRNA expression and inflammatory cytokines of IS patients (P > 0.050), but was significantly associated with HDL-C (recessive model: ß = - 0.14, 95 % CI: -0.24 to -0.03, P = 0.009). TLR5 rs5744174 polymorphism may have no impact on the stroke risk, gene expression and inflammatory cytokines, but may influence the HDL-C serum level of IS patients in Chinese Han population.


Assuntos
Citocinas/metabolismo , Regulação da Expressão Gênica , Lipídeos/sangue , Polimorfismo de Nucleotídeo Único/genética , Acidente Vascular Cerebral/genética , Acidente Vascular Cerebral/metabolismo , Receptor 5 Toll-Like/genética , Idoso , Alelos , Isquemia Encefálica/complicações , Estudos de Casos e Controles , China , Colesterol/sangue , Citocinas/genética , Feminino , Estudos de Associação Genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Fatores Sexuais , Acidente Vascular Cerebral/diagnóstico por imagem , Acidente Vascular Cerebral/etiologia
6.
Br J Nutr ; 114(6): 952-8, 2015 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-26283622

RESUMO

It has been shown that food insecurity is associated with poor diet quality and unfavourable health outcomes. However, little is known about the potential effects of food insecurity on the overall malnutrition status among children. In this study, we investigated the prevalence of food insecurity among 1583 elementary school students, aged 6-14 years, living in Chinese rural areas and examined its association with four malnutrition signs, including rickets sequelae, anaemia, stunting and wasting. Information on food security was collected via questionnaires. Rickets sequelae were assessed by an experienced paediatrician during the interview. Anaemia was determined by the WHO Hb thresholds adjusted by the local altitude. Weight and height were measured during the interview. Stunting and wasting were then evaluated according to WHO child growth standards (2007). We examined the association between food insecurity and the number of malnutrition signs (total number = 4), and the likelihood of having severe malnutrition (presence of 3+ signs), after adjusting for potential confounders, such as age, social-economic status and dietary intakes. During the previous 12 months, the overall prevalence of food insecurity was 6.1% in the entire studied population and 16.3% in participants with severe malnutrition. Participants with food insecurity had a slightly higher number of malnutrition signs (1.14 v. 0.96; P=0.043) relative to those who were food secure, after adjusting for potential confounders. Food insecurity was also associated with increased likelihood of having severe malnutrition (adjusted OR 3.08; 95% CI 1.47, 6.46; P=0.003). In conclusion, food insecurity is significantly associated with malnutrition among Chinese children in this community.


Assuntos
Fenômenos Fisiológicos da Nutrição do Adolescente , Fenômenos Fisiológicos da Nutrição Infantil , Dieta/efeitos adversos , Abastecimento de Alimentos , Desnutrição/epidemiologia , Saúde da População Rural , Desnutrição Aguda Grave/epidemiologia , Adolescente , Fenômenos Fisiológicos da Nutrição do Adolescente/etnologia , Anemia Ferropriva/etiologia , Criança , Fenômenos Fisiológicos da Nutrição Infantil/etnologia , China/epidemiologia , Estudos Transversais , Dieta/etnologia , Dieta/psicologia , Feminino , Abastecimento de Alimentos/economia , Transtornos do Crescimento/etiologia , Humanos , Masculino , Desnutrição/economia , Desnutrição/etnologia , Desnutrição/fisiopatologia , Inquéritos Nutricionais , Prevalência , Raquitismo/etiologia , Saúde da População Rural/etnologia , Desnutrição Aguda Grave/economia , Desnutrição Aguda Grave/etnologia , Desnutrição Aguda Grave/fisiopatologia , Síndrome de Emaciação/etiologia
7.
Wei Sheng Yan Jiu ; 42(4): 571-5, 2013 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-24024366

RESUMO

OBJECTIVE: To understand the nutritional status of pupils and to provide basic evidence for the nutritional interventions. METHODS: The subjects of the study were 2046 pupils from 9 primary schools in poor counties of Guangxi and Yunnan. The contents of the investigation included medical physical examination, haematoglobin determination and dietary survey. Nutritional Status was evaluated according to WHO growth standards. RESULTS: The prevalence of stunting and wasting in children from 6 to 14 years old in poor areas of Guangxi and Yunnan were 34.3% and 6.5% respectively. The anemia prevalence of them was 20.5%. The diet provided by the canteens of local school was mainly composed of plant food. Animal food was deficient. The cooking oil in local school was most of lard oil. The per pupil average daily intake of energy, protein, thiamin, riboflavin, vitamin C and calcium was 1491.29 kcal, 36.36 g, 179.15 ug, 0.57 mg, 0.37 mg, 53.67 mg and 163.14 mg respectively, which were lower than RNI or Al. CONCLUSION: The chronic malnutrition of pupils in poor areas is still serious. The variety of food provided by the canteens of local school is quite single, and the quality of the meals is low. Intakes of energy, protein and micronutrient from school diet are insufficient for pupils.


Assuntos
Inquéritos sobre Dietas , Desnutrição/epidemiologia , Estado Nutricional , Anemia/epidemiologia , Criança , China/epidemiologia , Feminino , Humanos , Masculino , Áreas de Pobreza , Prevalência , Instituições Acadêmicas , Estudantes
8.
Zhonghua Nan Ke Xue ; 14(5): 387-90, 2008 May.
Artigo em Chinês | MEDLINE | ID: mdl-18572853

RESUMO

OBJECTIVE: To evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility. METHODS: Computer-aided sperm analysis (CASA) was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1, 3, 5 and 7 hours. RESULTS: The curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours. CONCLUSION: The sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.


Assuntos
Capacitação Espermática/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Animais , Cobaias , Cinética , Masculino , Análise Numérica Assistida por Computador
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