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1.
Methods Mol Biol ; 2170: 1-18, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32797447

RESUMO

Application of the CRISPR-Cas prokaryotic immune system for single-stranded RNA targeting will have significant impacts on RNA analysis and engineering. The class 2 Type VI CRISPR-Cas13 system is an RNA-guided RNA-nuclease system capable of binding and cleaving target single-stranded RNA substrates in a sequence-specific manner. In addition to RNA interference, the Cas13a system has application from manipulating RNA modifications, to editing RNA sequence, to use as a nucleic acid detection tool. This protocol uses the Cas13a ortholog from Leptotrichia buccalis for transient expression in plant cells providing antiviral defense. We cover all the necessary information for cloning the Cas13 protein, crRNA guide cassette, performing transient Agrobacterium-mediated expression of the necessary Cas13a components and target RNA-virus, visualization of virus infection, and molecular quantification of viral accumulation using quantitative PCR.

2.
Can J Microbiol ; 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33064956

RESUMO

Directional stress is an effective measure to evolve community structure and improve bioactivity of pit mud (PM). In this study, adding fortified Daqu in artificial PM (APM) was to disturb the microbial community and affect the metabolites furthermore. To evaluate the effect of fortified Daqu on culturing APM, microbial communities of APMs with/without adding fortified Daqu were investigated by fluorescence in situ hybridization and Illumina Miseq. These results indicated that microbes (Clostridium sp., Clostridium kluyveri, hydrogenotrophic methanogens, and acetotrophic methanogens) related to the production of key aroma compounds increased notably when fortified Daqu was added. Especially the hydrogenotrophic and acetotrophic methanogens increased by 5.19- and 4.63-fold after 30-days' culture. Then metabolites (organic acids, volatile compounds) were also analyzed by HPLC and HS-SPME-GC-MS. Results showed that the content of butyric acid and hexanoic acid was significantly higher when adding fortified Daqu. What's more, the proportion of esters and phenols were higher compared with the APM without adding fortified Daqu as well. The microbial compositions of APMs with/without adding fortified Daqu were observed in this study, which indicated the microbial community evolving in functional community in favor of liquor-brewing and suggested a novelty process was developed by disturbing the community diversity.

3.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 28(5): 1468-1473, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33067939

RESUMO

OBJECTIVE: To investigate the diagnosis and treatment of esophageal granulocytic sarcoma derived from chronic myelocytic leukemia (CML). METHODS: The clinical manifestations, diagnosis and treatment of 1 case of esophageal granulocytic sarcoma secondary from chronic myelocytic leukemia were retrospectively analyzed and the related literature was reviewed. RESULTS: The patient was a 72-year-old woman with poststernal pain accompanied by general weakness. Gastroscopy was performed in a local hospital. At the same time, the increase of peripheral blood leucocytes was obvious. Under gastroscopy, 1.0 cm×0.5 cm irregular protuberance was found at 28 cm from the esophagus to the incisor teeth, and the surface was covered with erosion and a small amount of blood. Pathological results showed that heterotypic lymphoid cell infiltration, cytoplasmic red staining and more neutrophils were seen. Immunohistochemical staining results showed that AE1/AE3, CK5/6 and p63 displayed squamous epithelium (+); atypical lymphoid cells CD20-, CD23-, CD3-, CD5-, CD79a-, MP0+, Ki-67+ (80%) were observed; FISH examination showed positive expression of BCR/ABL. The patient was further examined on myelogran and was diagnosed as chronic myelocytic leukemia with esophageal granulocytic sarcoma. Imatinib was given orally and the patient was followed up in the clinic. CONCLUSION: Esophageal granulocytic sarcoma is rare in clinic, its clinical symptoms are not specific. Gastroscopy should be routinely screened for esophageal discomfort, and the esophageal granulocytic sarcoma derived from CML is treated according to the therapeutic regimen of the acute transformation of chronic myelocytic leukemia.

4.
ACS Nano ; 2020 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-33012161

RESUMO

Transition-metal phosphates/phosphides possess promising theoretical electrochemical characteristics and exhibit great potential in advanced supercapacitors. Unfortunately, limited by the processing techniques and overall structure, their specific capacity and rate performance are still unsatisfactory. Herein, we report the fabrication of transition-metal phosphate electrodes with an ultrathin sheetlike array structure by one-step electrodeposition at room temperature. As a proof-of-concept, a transition-metal phosphate member of NiCo(HPO4)2·3H2O with an ultrathin nanosheet structure (thickness ∼2.3 nm) was synthesized and investigated. The as-prepared NiCo(HPO4)2·3H2O electrode showcases an ultrahigh specific capacity of 1768.5 C g-1 at 2 A g-1 (the highest value for transition-metal phosphates/phosphides reported to date), superb rate performance of 1144.8 C g-1 at 100 A g-1, and excellent electrochemical stability. Moreover, the transition-metal phosphate nanosheet array can be uniformly deposited on various conductive substrates, demonstrating the generality of our strategy. Therefore, this simple electrodeposition strategy provides an opportunity to fabricate ultrathin transition-metal phosphate nanosheet materials that can be used for energy storage/conversion, electrocatalysis, and other electrochemical energy-related devices.

5.
Ann Emerg Med ; 76(4): e59-e60, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33012388
6.
ISA Trans ; 2020 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-33041010

RESUMO

This paper deals with the sliding mode stabilization for chaotic systems. In the system under consideration, the nonlinear function is one-sided Lipschitz with quadratic inner-boundedness. Specifically, a non-fragile sliding mode surface is constructed, and the sufficient condition for the convergence is derived. Then, a new feedback law is proposed to enable the state trajectories of the closed-loop system to reach the sliding mode surface in finite time. Finally, an example in the background of the unified chaos system is simulated to show the validation of the designed controller.

7.
J Exp Clin Cancer Res ; 39(1): 207, 2020 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-33023613

RESUMO

BACKGROUND: Accumulating evidence indicates that long non-coding RNAs (lncRNAs) acting as crucial regulators in tumorigenesis. However, its biological functions of lncRNAs in colorectal cancer (CRC) have not been systematically clarified. METHODS: An unbiased screening was performed to identify disregulated lncRNAs revealed to be implicated in CRC carcinogenesis according to an online-available data dataset. In situ hybridization (ISH), RT-qPCR and RNA fluorescence in situ hybridization (RNA-FISH) were applied to detect RP11-757G1.5 expression in CRC tissues and cell lines. The associations of RP11-757G1.5 with clinicopathological characteristics were analyzed. Their effects on prognosis were analyzed by the Kaplan-Meier analysis, Log-rank test, Univariate and Multivariate Cox regression analysis. The potential biological function of RP11-757G1.5 in CRC was investigated by Colony formation, Edu cell proliferation, Flow cytometry, Wound healing and Transwell assays. Bioinformatics binding site analysis, Luciferase reporter assay, Ago2 immunoprecipitation assays, RNA pull-down assay, RT-qPCR and Western blotting were utilized to demonstrate the mechanism of RP11-757G1.5 acts as a molecular sponge of miR-139-5p to regulate the expression of YAP1. Finally, we further explore the potential role of RP11-757G1.5 in CRC orthotopic xenografts in vivo. RESULTS: We discovered a novel oncogenic lncRNA RP11-757G1.5, that was overexpressed in CRC tissues, especially in aggressive cases. Moreover, up-regulation of RP11-757G1.5 strongly correlated with poor clinical outcomes of patients with CRC. Functional analyses revealed that RP11-757G1.5 promoted cell proliferation in vitro and in vivo. Furthermore, RP11-757G1.5 stimulated cell migration and invasion in vitro and in vivo. Mechanistic studies illustrated that RP11-757G1.5 regulated the expression of YAP1 through sponging miR-139-5p and inhibiting its activity thereby promoting CRC progression and development. CONCLUSIONS: Altogether, these results reveal a novel RP11-757G1.5/miR-139-5p/YAP1 regulatory axis that participates in CRC carcinogenesis and progression.

8.
J Mater Chem B ; 8(38): 8768-8780, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33026387

RESUMO

Hydrogels have been widely used in wound healing treatment over the past decade. Injectable hydrogels have become a major research focus due to their unique advantages. Compared to traditional hydrogels, injectable hydrogels have good fluidity. When injected into the wound as a solution, they form a gel in situ that can fill the wound in three dimensions. This enables them to reach deep and irregular wounds that traditional hydrogels cannot fill. Injectable hydrogels greatly reduce the need for invasive surgery and are well-suited for chronic wound repair. This review article categorizes hydrogels that are commonly used in chronic wound repair according to their sources and reviews the current applications of the different types of injectable hydrogels in chronic wound repair.

9.
Small ; : e2004843, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33006209

RESUMO

Bimetallic phosphate electrocatalysts on carbon-cloth support are among the most promising industry-relevant solutions for electrocatalytic hydrogen production. To address the persistent issue of hetero-phase interfacing on carbon support while ensuring high activity and stability, a low-cost, high-performance hydrogen evolution reaction (HER) electrocatalyst is developed. Bi-phase Ni12 P5 -Ni4 Nb5 P4 nanocrystals with rich heterointerfaces and phase edges are successfully fabricated on carbon cloth (CC), which is enabled by intentional defect creation by atmospheric pressure dielectric barrier discharge (DBD) plasma (PCC). The obtained Ni12 P5 -Ni4 Nb5 P4 /PCC electrocatalyst exhibits excellent HER performance, heralded by the low overpotentials of 81 and 287 mV for delivering current densities of 10 (j10 ) and 500 (j500 ) mA cm-2 , respectively. Meanwhile, the Ni12 P5 -Ni4 Nb5 P4 /PCC maintains spectacular catalytic activity at high current density region (>j615 ), which outperformed the industry-relevant benchmark Pt/C/PCC catalyst. The catalyst grown on the plasma-treated support shows remarkably longer operation and ultra-stable electrocatalytic characteristics over 100 h continuous operation. Ab initio numerical simulations reveal that Ni atoms exposed in the heterointerfaces act as the main catalytically active centers for HER.

10.
PLoS Pathog ; 16(10): e1008461, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33002089

RESUMO

The induction of an interferon-mediated response is the first line of defense against pathogens such as viruses. Yet, the dynamics and extent of interferon alpha (IFNα)-induced antiviral genes vary remarkably and comprise three expression clusters: early, intermediate and late. By mathematical modeling based on time-resolved quantitative data, we identified mRNA stability as well as a negative regulatory loop as key mechanisms endogenously controlling the expression dynamics of IFNα-induced antiviral genes in hepatocytes. Guided by the mathematical model, we uncovered that this regulatory loop is mediated by the transcription factor IRF2 and showed that knock-down of IRF2 results in enhanced expression of early, intermediate and late IFNα-induced antiviral genes. Co-stimulation experiments with different pro-inflammatory cytokines revealed that this amplified expression dynamics of the early, intermediate and late IFNα-induced antiviral genes can also be achieved by co-application of IFNα and interleukin1 beta (IL1ß). Consistently, we found that IL1ß enhances IFNα-mediated repression of viral replication. Conversely, we observed that in IL1ß receptor knock-out mice replication of viruses sensitive to IFNα is increased. Thus, IL1ß is capable to potentiate IFNα-induced antiviral responses and could be exploited to improve antiviral therapies.

11.
J Hazard Mater ; 404(Pt A): 124121, 2020 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-33011633

RESUMO

Microplastics (MPs) can be easily taken up by a wide range of aquatic animals and cause blockage of the digestive tract leading to starvation. Meanwhile, aquatic organisms are facing threats posed by food restriction in both wild and cultured environment. Little knowledge, however, exists on how MPs interact with food conditions to affect aquatic animals. Here, koi carp were exposed to polystyrene MPs (0, 100 or 1000 µg/L) under controlled feeding (satiated or starved) for 30 or 60 days. MPs reduced and interacted synergistically with food conditions on growth after 30 days but antagonistically after 60 days. MPs reduced crude lipid and carbohydrate but increased and antagonistically interacted with feeding conditions on crude protein. Food conditions interacted with MPs on C, N and P but stoichiometric responses were decoupled with macromolecules changes. Food conditions antagonistically interacted with MPs on δ13C after 60 days. Linear discriminant analysis revealed that C:P and N:P were the two most important measured parameters accounting for the response of koi towards MPs and food restriction, presenting an antagonistic interaction of MPs and food status with the prolonged exposure duration.

12.
Clin Infect Dis ; 2020 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-32920641

RESUMO

BACKGROUND: A live-attenuated Plasmodium falciparum (Pf) sporozoite (SPZ) vaccine (PfSPZ Vaccine) has shown up to 100% protection against controlled human malaria infection (CHMI) using homologous parasites (same Pf strain as in the vaccine). Using a more stringent CHMI, with heterologous parasites (different Pf strain), we assessed the impact of higher PfSPZ doses, a novel multi-dose prime regimen, and a delayed vaccine boost upon vaccine efficacy. METHODS: Four groups of 15 healthy, malaria-naïve adults were immunized. Group (Grp) 1 received five doses of 4.5x10 5 PfSPZ (days 1, 3, 5, 7; week 16). Grps 2, 3 and 4 received three doses (weeks 0, 8, 16) with Gp 2 receiving 9.0×10 5/dose, Grp 3 receiving 18.0×10 5/dose, and Grp 4 receiving 27.0×10 5 for dose 1 and 9.0×10 5 for doses 2 and 3. VE was assessed by heterologous CHMI after 12 or 24 weeks. Volunteers not protected at 12 weeks were boosted prior to repeat CHMI at 24 weeks. RESULTS: At 12-week CHMI, 6/15 (40%) Group 1 (P=0.04), 3/15 (20%) Group 2 vs. 0/8 controls remained aparasitemic. At 24-week CHMI, 3/13 (23%) Group 3, 3/14 (21%) Group 4 vs. 0/8 controls remained aparasitemic (Groups 2-4, VE not significant). Post-boost, 9/14 (64%) vs. 0/8 controls remained aparasitemic (3/6 Group 1, P=0.025; 6/8 Group 2, P=0.002). CONCLUSIONS: Four stacked, priming injections (multi-dose priming) showed 40% VE against heterologous CHMI, while dose escalation of PfSPZ using single dose priming was not significantly protective. Boosting unprotected subjects improved VE at 24 weeks to 64%.

13.
ACS Nano ; 2020 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-32877169

RESUMO

The thermal conductivity measurement of metal-organic frameworks (MOFs), which plays an important role in thermal management of MOF-based gas separation, storage, and thermal energy conversion (e.g., adsorption heat pumps), has been a challenging task for decades. However, the direct thermal conductivity measurement of a single-crystal MOF is currently limited by their small crystal sizes, since no sophisticated approach has ever been reported. In this study, the Raman-resistance temperature detectors (Raman-RTDs) method was developed for in situ measuring of the thermal conductivity of single-crystal ZIF-8, whose system error resulting from the thermal contact resistance between sample and RTDs can be eliminated. According to the dependence of thermal resistance of MOF crystals on the laser spot location, the thermal conductivities of polycrystalline and single-crystal ZIF-8 were derived to be 0.21 ± 0.03 and 0.64 ± 0.09 W/(m·K), respectively. The proposed in situ thermal conductivity measurement method may be further extended to other types of microscale particles.

14.
J Int Med Res ; 48(9): 300060520931618, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32962487

RESUMO

OBJECTIVE: To compare the efficacy of ranibizumab plus fufang xueshuantong capsule (cFXST) with the efficacy of ranibizumab alone in treatment of exudative age-related macular degeneration. METHODS: This prospective, randomized, controlled, pilot study included 38 eyes from 38 patients with exudative age-related macular degeneration (AMD) that were randomly allocated into two cohorts of 19 eyes each: ranibizumab (Cr) and ranibizumab plus cFXST (Cfr). All patients received three monthly injections of ranibizumab. Patients in Cfr also received daily oral supplementation of cFXST. Best corrected visual acuity (BCVA) and thickness of the choroidal neovascularization-pigment epithelial detachment (CNV-PED) complex (measured by optical coherence tomography) were recorded at baseline and at 1 and 3 months after the first intravitreal injection of ranibizumab. RESULTS: In the Cfr, the CNV-PED complex thickness was reduced by 31.7% and 36.1% at 1 and 3 months, respectively; these reductions were significantly greater than the 19.7% and 24.2% reductions in the Cr. BCVA improvement was significantly greater in the Cfr than in the Cr after 3 months; the proportion of patients with functional response was also greater in the Cfr than in the Cr (16/16 vs. 8/17). CONCLUSION: Oral cFXST increases the efficacy of short-term ranibizumab treatment for exudative AMD.

15.
Anal Chem ; 2020 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-32986405

RESUMO

DNA damage is one of major culprits in many complex diseases; thus, there is great interest in the discovery of novel lead compounds regulating DNA damage. However, there remain plenty of challenges to evaluate DNA damage through counting the amount of intranuclear foci. Herein, a deep-learning-based open-source pipeline, FociNet, was developed to automatically segment full-field fluorescent images and dissect DNA damage of each cell. We annotated 6000 single-nucleus images to train the classification ability of the proposed computational pipeline. Results showed that FociNet achieved satisfying performance in classifying a single cell into a normal, damaged, or nonsignaling (no fusion-protein expression) state and exhibited excellent compatibility in the assessment of DNA damage based on fluorescent foci images from various imaging platforms. Furthermore, FociNet was employed to analyze a data set of over 5000 foci images from a high-content screening of 315 natural compounds from traditional Chinese medicine. It was successfully applied to identify several novel active compounds including evodiamine, isoliquiritigenin, and herbacetin, which were found to reduce 53BP1 foci for the first time. Among them, isoliquiritigenin from Glycyrrhiza uralensis Fisch. exerts a significant effect on attenuating double strand breaks as indicated by the comet assay. In conclusion, this work provides an artificial intelligence tool to evaluate DNA damage on the basis of microscopy images as well as a potential strategy for high-content screening of active compounds.

16.
Anticancer Res ; 40(9): 4969-4978, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32878785

RESUMO

BACKGROUND/AIM: Traditional Chinese medicine (TCM) Brucea javanica (BJO) has shown anti-proliferation efficacy on human carcinoma cells in vitro. The aim of the present study was to evaluate for the first time the efficacy of BJO combined with the first-line chemotherapeutic drug gemcitabine (GEM) on tumor growth-inhibition and survival in a pancreatic cancer patient-derived orthotopic xenograft (PDOX) mouse model. MATERIALS AND METHODS: The pancreatic cancer tumor fragment originated from a patient at the Hefei First People's Hospital (Anhui, PR China). The surgical specimen was transplanted orthotopically in nude mice using surgical orthotopic implantation (SOI). All mice were randomized and assigned to 5 groups: G1: saline vehicle (0.1ml per mouse, oral, once per day); G2: GEM [100 mg/kg, intraperitoneal (i.p), twice per week]; G3: GEM+BJO [100 mg/kg GEM, i.p, twice per week+1g/kg BJO, oral, once per day (qd)]; G4: BJO (1g/kg, oral, qd). Group 5 and Group 6 were used to observe survival [G5: saline vehicle (0.1ml per mouse, oral, qd), G6: BJO (1g/kg, oral, qd)]. Body weight and tumor volume were measured twice per week. TUNEL staining was used to determine apoptosis. RESULTS: The combination of GEM + BJO resulted in a reduced tumor growth rate (p<0.05) and greater apoptosis (p<0.05) compared to the vehicle control and GEM monotherapy. In addition, the BJO-treated group showed a statistically significant increase in survival compared to the vehicle control (p<0.05). CONCLUSION: BJO is a promising non-toxic TCM to effectively treat pancreatic cancer, both as monotherapy and in combination with first-line GEM therapy.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Brucea/química , Desoxicitidina/análogos & derivados , Neoplasias Pancreáticas/tratamento farmacológico , Animais , Apoptose/efeitos dos fármacos , Desoxicitidina/uso terapêutico , Sinergismo Farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Humanos , Camundongos , Camundongos Nus , Neoplasias Pancreáticas/patologia , Óleos Vegetais/uso terapêutico , Análise de Sobrevida , Carga Tumoral/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
17.
IEEE Trans Cybern ; PP2020 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-32936761

RESUMO

This article tackles the problem of multilabel learning with missing labels. For this problem, it is widely accepted that label correlations can be used to recover the ground-truth label matrix. Most of the existing approaches impose the low-rank assumption on the observed label matrix to exploit label correlations by decomposing it into two matrices, which describe the latent factors of instances and labels, respectively. The quality of these latent factors highly influences the recovery of ground-truth labels and the construction of the multilabel classification model. In this article, we propose recovering the ground-truth label matrix by regularized matrix factorization. Specifically, the latent factors of instances are regularized by the local topological structure derived from the feature space, which can be further used to induce an effective multilabel model. Moreover, the latent factors of labels and the label correlations are mutually adapted via label manifold regularization. In this way, the recovery of the ground-truth label matrix and the construction of the multilabel classification model are optimized jointly and can benefit from the regularized matrix factorization. Extensive experimental studies show that the proposed approach significantly outperforms the state-of-the-art algorithms on both full-label and missing-label data.

19.
Complement Ther Med ; 52: 102476, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32951726

RESUMO

OBJECTIVE: Systematically assessing the safety and effectiveness of spraying rhubarb powder solution under gastroscope for the treatment of acute non-varicose upper gastrointestinal bleeding, and confirmation for further clinical research and application. METHODS: We searched the following databases up till November 2019: PubMed, the Cochrane Library, EMBASE, CNKI, WanFang Data, VIP and SinoMed. Randomized controlled trials (RCTs) were used to compare the curative effect of spraying rhubarb powder solution with other drugs under gastroscope for the treatment of acute non-varicose upper gastrointestinal bleeding. RESULTS: Out of 171 articles, 14 RCTs involving 1493 patients were included. All control groups included in the RCTs were treated with norepinephrine solution. The hemostatic effect of spraying rhubarb powder solution under gastroscope was examined for 24 h at high concentration (0.1 g/mL). The hemostatic effect at higher conc. (0.1 g/mL) found far more better than low conc.(RR = 1.48;95 %CI:1.25,1.75;P﹤0.00001) (0.03 g/mL)as homeostatic effect at low conc.is same that of norepinephrine solution (RR = 1.02;95 %CI:0.94,1.10;P = 0.62). Moreover within 48 h, rhubarb powder solution with 0.1 g/mL or 0.15 g/mL conc. have of significantly higher hemostatic effects than norepinephrine solution (RR = 1.18;95 % CI: 1.08, 1.30;P = 0.0003). Occurrence of rebleeding event within 48 h after successful hemostasis (RR = 0.42;95 %CI:0.24,0.74;P = 0.003) reduced exceptionally. After that the hemostatic effect of rhubarb powder solution with 0.1 g/mL conc.examined within 72 h again exhibited significant improvement than norepinephrine solution (RR = 1.19;95 %CI:1.12,1.26;P < 0.00001). On par with immediate hemostasis time, rhubarb powder solution took unprecedented less time than norepinephrine solution;(MD=-5.56S;95 %CI:-6.16, -4.95;P﹤0.00001). Additionally, the adverse reaction produced by rhubarb powder solution is much lower than norepinephrine solution (RR = 0.22;95 %CI:0.11,0.42;P < 0.00001). CONCLUSIONS: According to meta-analysis, Spraying rhubarb powder solution under gastroscope in the treatment of acute non-varicose upper gastrointestinal bleeding is superior to norepinephrine solution in improving hemostasis effect. Shortening immediate hemostasis time and reducing rebleeding,and is safe to use. Based on the results of this study, physicians can treat patients with acute non-varicose upper gastrointestinal bleeding by spraying rhubarb powder solution under gastroscope according to the patients' condition.However, the sample size included in this study is small and of substandard quality qu, and a large sample size clinical trial with strict design and normative report is needed to verify the safety and efficacy of rhubarb powder solution under gastroscope for acute non-varicose upper gastrointestinal bleeding.

20.
Hemoglobin ; : 1-5, 2020 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-32811243

RESUMO

The aim of this study was to investigate a family with nonhomologous sequence recombination of HBA1 and HBA2 genes and provide a favorable basis for genetic counseling and eugenics. Peripheral blood of family members was collected. Hematological parameters were determined by an automated cell counter and hemoglobin (Hb) analysis was performed using high performance liquid chromatography (HPLC). Villus samples were taken for prenatal diagnosis (PND). Gap-polymerase chain reaction (gap-PCR) and reverse dot-blot were used for thalassemia genotyping. DNA sequencing was used to analyze the gene sequence of HBA1 (α1-globin) and HBA2 (α2-globin). The nonhomologous sequence recombination allelic variant of HBA1 and HBA2 genes were identified, namely, a gene conversion on the HBA2 gene called α12 (HBA12). The α12 allele consists primarily of the HBA2 gene sequence except for a segment of the IVS-II in which HBA2-specific sequences have been replaced by HBA1-specific sequences. The following genotypes were observed: - -SEA/αα12 (Southeast Asian deletion), αα/αα12 and αQSα/αα12 (Hb Quong Sze or Hb QS; HBA2: c.377T>C), and all manifested as small cell hypochromic anemia. To find the α12 allele in the Chinese population and clarify the influence of the α12 allele and its common inheritance with abnormal Hb and α-thalassemia (α-thal) on α-globin gene expression can help guide clinical diagnosis and genetic counseling.

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