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1.
Mol Med Rep ; 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31746415

RESUMO

Individual differences in the response to fentanyl, which may be caused by different concentrations of the drug in the central nervous system, can complicate analgesic treatment. It has been reported that the organic anion transporting polypeptide (OATP) at the blood­brain barrier (BBB) in Sprague­Dawley rats may serve an important role in the transport of fentanyl across the BBB. However, whether human OATP can transport fentanyl has thus far not been reported. The present study aimed to establish a 293 cell line stably overexpressing OATP1A2, and to determine whether OATP1A2 is able to transport fentanyl across the plasma membrane. Initially, 293 cells were transfected with an OATP1A2­expressing plasmid (referred to as 293­OATP1A2 cells), and single colonies were selected and characterized following geneticin treatment. Subsequently, reverse transcription­quantitative polymerase chain reaction and western blot analyses were conducted to verify the transfection efficiency. Furthermore, treatment of 293­OATP1A2 cells with different concentrations of fexofenadine (FEX) and fentanyl was performed to investigate the transport function of OATP1A2 in 293 cells. FEX and fentanyl uptake experiments were also performed with naringenin, an inhibitor of OATP1A2. The results indicated that FEX and fentanyl uptake was significantly increased in 293­OATP1A2 cells compared with that in the control­transfected cells. The 293­OATP1A2­mediated uptake of FEX at concentration of 100 nM FEX was ~10­fold higher than that of 293­VC cells. The 293­OATP1A2­mediated uptake of fentanyl (100 nM) was 5.1­fold higher compared with that in 293­VC cells. In 293­OATP1A2 cells, the uptake of FEX without OATP1A2 inhibitor naringenin (100 µg/ml) was 2.8­fold higher compared with that in the presence of naringenin, and the uptake of fentanyl without naringenin was 7.3­fold higher compared with that in the presence of naringenin (100 µg/ml). In conclusion, 293 cells that overexpressed OATP1A2 were successfully constructed, and OATP1A2 was revealed to mediate fentanyl uptake in the cultured cells.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31623306

RESUMO

This study aimed to investigate the association between environmental exposure to tobacco smoke (ETS) during early life and astigmatism in Chinese preschool children. In this cross-sectional study, information concerning prenatal and postnatal ETS exposure at three stages of early life (during pregnancy, from birth to one year and from one to three years), visual problems of children and parents (including a confirmed diagnosis of astigmatism), socio-demographics and perinatal characteristics were obtained from 27,890 parent-reported questionnaires. Logistic regression analyses were undertaken to yield adjusted odds ratios (OR) for assessing their associations. After adjusting for the potential confounders, children were more likely to exhibit astigmatism when they were exposed to ETS during pregnancy + from one to three years [OR (95% CI) = 1.37 (1.02, 1.84)], or from birth to one year + from one to three years [OR (95% CI) = 1.36 (1.11, 1.66)], or during pregnancy + from birth to one year + from one to three years old [OR (95% CI) = 1.29 (1.16, 1.45)], compared to children without ETS exposure at any stage of early life. In Chinese preschool children, prenatal and postnatal astigmatism was associated with ETS exposure; the greater the ETS dose, the greater the astigmatism risk.

3.
Eur J Med Chem ; 181: 111520, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31404863

RESUMO

A series of novel triazole nucleobase analogues containing steroidal/coumarin/quinoline moieties have been synthesized based on copper-catalyzed azide-alkyne cycloaddition (CuAAC). The anti-cancer activity of the new triazole nucleobase analogues was studied in gastric cancer cell lines (MGC-803, SGC-7901) and normal gastric epithelial cells (GES-1) in vitro. Some of the synthesized compounds could significantly inhibit the proliferation of these tested cancer cells. Among the tested compounds, compound 20c demonstrated good anti-proliferation activity against MGC-803 cells (IC50 = 1.48 µM) and SGC-7901 (IC50 = 2.28 µM) cells as well as the best selectivity between the cancer and normal cells. Further mechanistic studies indicated that compound 20c could down-regulate the expression of TGF ß1 both in the tested gastric cancer cell lines and inhibit the cell migration and invasion. The results of the study indicate that compound 20c could be used as a promising skeleton for anti-gastric cancer agents with improved efficacy and less side effects.

4.
Steroids ; 150: 108431, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31229507

RESUMO

ß-Triazoly enones are biologically interesting scaffolds, incorporation of such scaffolds into the steroid nucleus may generate new bioactive steroids and further enrich structural types of steroids. In this work, a series of new steroidal ß-triazoly enones were synthesized based on click chemistry and Claisen-Schmidt condensation reaction and further evaluated for their antiproliferative activity against a panel of cancer cells. Most of these compounds showed better potency against PC-3 and MGC-803 cells. Particularly, compound 5a inhibited PC-3 and MGC-803 cells potently with the IC50 values of 1.61 and 1.16 µM, respectively, and was less toxic toward GES-1 with an IC50 value of 20.72 µM. Further mechanistic studies showed that compound 5a inhibited migration and invasion of MGC-803 and PC-3 dose-dependently. Treatment with compound 5a varied mRNA levels and protein expression of EMT markers in both cells. Collectively, the steroidal ß-triazoly enones could be potentially utilized to develop new anticancer agents with the ability of inhibiting cell migration and invasion.

5.
Sci Rep ; 9(1): 7272, 2019 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-31086227

RESUMO

Understanding signals in the microenvironment that regulate endothelial cell behavior are important in tissue engineering. Although many studies have examined the cellular effects of nanotopography, no study has investigated the functional regulation of human endothelial cells grown on nano-sized gradient hole substrate. We examined the cellular response of human umbilical vein endothelial cells (HUVECs) by using a gradient nanohole substrate (GHS) with three different types of nanohole patterns (HP): which diameters were described in HP1, 120-200 nm; HP2, 200-280 nm; HP3, 280-360 nm. In results, HP2 GHS increased the attachment and proliferation of HUVECs. Also, gene expression of focal adhesion markers in HUVECs was significantly increased on HP2 GHS. In vitro tube formation assay showed the enhancement of tubular network formation of HUVECs after priming on GHS compared to Flat. Furthermore, leukocyte adhesion was also reduced in the HUVECs in a hole-diameter dependent manner. To summarize, optimal proliferations with reduced leukocyte adhesion of HUVECs were achieved by gradient nanohole substrate with 200-280 nm-sized holes.

6.
Taiwan J Obstet Gynecol ; 58(1): 168-172, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30638474

RESUMO

OBJECTIVE: Sleep disturbance is a common issue that can arise from emotional distress. Women who received the complicated invasive in-vitro fertilization (IVF) procedures can be stressful. The purpose of this study is to understanding the association between emotional distress (measured as anxiety and depression) and sleep quality for women undergoing IVF treatment. MATERIALS AND METHODS: This research was of a cross-sectional questionnaire design. Questionnaires were completed during the controlled ovarian hyperstimulation of IVF treatment. A total of 97 participants complete the survey. The questionnaires included basic personal information, The Chinese Traditional Childbearing Attitude Questionnaire, Pittsburgh Sleep Quality Index (PSQI), and the Beck depression and anxiety scales. RESULTS: A total of 42.9% and 30% of the participants were determined to be anxious and depressed, respectively. Some participants (18.8%) required more than 30 min to fall asleep, and 56.2% of the participants had less than 7 h of sleep, 43.6% of the participants had less than 85% of sleep efficiency, and 43.3% of the participants were determined with poor sleep quality (PSQI>5). The linear regression analysis indicated that the score of the Beck anxiety index was significantly associated with sleep quality (F = 44.85, p = .000, adjust R2 = 49.4%). CONCLUSION: This study revealed that some women when receiving early stage IVF treatments were anxious, depressed, and have sleep disturbance. Anxiety was significantly associated with sleep quality. It is suggested that medical care professional should understand these common issues when assisting women undergoing these complicated reproductive treatments.


Assuntos
Ansiedade/complicações , Depressão/complicações , Fertilização In Vitro/psicologia , Distúrbios do Início e da Manutenção do Sono/etiologia , Distúrbios do Início e da Manutenção do Sono/psicologia , Estresse Psicológico/complicações , Adulto , Ansiedade/epidemiologia , Ansiedade/psicologia , Estudos Transversais , Depressão/epidemiologia , Depressão/psicologia , Feminino , Fertilização In Vitro/efeitos adversos , Humanos , Distúrbios do Início e da Manutenção do Sono/epidemiologia , Estresse Psicológico/epidemiologia , Inquéritos e Questionários
7.
Taiwan J Obstet Gynecol ; 58(1): 68-71, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30638484

RESUMO

OBJECTIVE: To evaluate the effect of far-infrared radiation on postpartum perineal pain and sexual function in primiparous women undergoing an episiotomy and 2nd degree perineal laceration. MATERIALS AND METHODS: From May 2016 to May 2017, primiparous women with term pregnancies and vaginal deliveries with an episiotomy due to obstetric indications and 2nd degree perineal lacerations were invited to participate in this study. Women were randomly assigned into a far-infrared (FIR) group and a control group. Visual Analogue Scale (VAS) was used to evaluate the perineal pain immediately postpartum, and at 1 week and 6 weeks postpartum. Pelvic Organ Prolapse Urinary Incontinence Sexual Questionnaire (PISQ-12) was used to evaluate sexual function at 6 weeks, 3 months and 6 months postpartum. RESULTS: The data of the control group (n = 22) and FIR group (n = 18) were analyzed. Most of the women had minor perineal pain one week after delivery (1.1 ± 0.9 in control group vs 1.4 ± 1.5 in FIR group) and there was no significant difference between groups. The FIR group had a higher PISQ-12 total score at 3 months (35.4 ± 6.4 vs 34.7 ± 5.7) and 6 months (36.4 ± 5.6 vs 35.6 ± 5.7) postpartum compared with the control group but there was no statistically significant difference. CONCLUSION: Our study did not show any additional benefit of postpartum far-infrared radiation on primiparous women undergoing an episiotomy and 2nd degree perineal lacerations.


Assuntos
Dispareunia/etiologia , Episiotomia/efeitos adversos , Lacerações/radioterapia , Dor Pélvica/prevenção & controle , Períneo/lesões , Cicatrização , Adulto , Parto Obstétrico/efeitos adversos , Dispareunia/prevenção & controle , Feminino , Humanos , Período Pós-Parto , Gravidez , Estudos Prospectivos
8.
Res Nurs Health ; 42(1): 48-60, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30681165

RESUMO

In this study, the Delphi method was used to develop evidence-based indicators of intensive care unit (ICU) nursing quality of care in China. Nursing quality indicators reflect elements of patient care that are directly affected by nursing practice. A comprehensive literature search identified 2,857 potentially relevant articles. From the 50 articles that were included in this study, researchers identified 38 commonly used nursing quality indicators. A panel of experts reduced these to 20, which were then subjected to two rounds of Delphi discussion by a different panel, and a final consensus was achieved. The 20 indicators were grouped into three dimensions: structure, process, and outcome (including adverse consequences). The agreement among the experts for the 20 indicators was high. These evidence-based nursing quality indicators provide for ease in data collection and a basis for clinical application and improvement in the quality of ICU nursing throughout China.


Assuntos
Enfermagem de Cuidados Críticos/normas , Unidades de Terapia Intensiva/normas , Recursos Humanos de Enfermagem no Hospital/normas , Indicadores de Qualidade em Assistência à Saúde/normas , China , Técnica Delfos , Enfermagem Baseada em Evidências , Humanos , Papel do Profissional de Enfermagem
9.
Zhongguo Dang Dai Er Ke Za Zhi ; 20(12): 990-993, 2018 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-30572986

RESUMO

OBJECTIVE: To investigate the screening indices and their cut-off values for full-term neonates carrying ß-thalassemia gene. METHODS: A retrospective analysis was performed for the clinical data of 1 193 full-term neonates who underwent ß-thalassemia screening (hemoglobin analysis with dried blood spots on neonatal heel blood filter paper and mutation detection of 17 ß-globin genes). A multivariate logistic regression analysis was used to investigate the association between screening indices and ß-thalassemia gene, and the receiver operating characteristic (ROC) curve was used to analyze the value of screening indices in determining the presence or absence of ß-thalassemia gene. RESULTS: Of the 1 193 neonates, 638 carried ß-thalassemia gene. Of the 1 193 neonates, 637 (53.39%) had no HbA2, among whom 310 carried ß-thalassemia gene and 327 did not carry this gene; 556 (46.61%) had HbA2, among whom 328 carried ß-thalassemia gene and 228 did not carry this gene. As for the neonates without HbA2, the ß-thalassemia gene group had a significantly lower HbA level and a significantly higher HbF level than the ß-thalassemia gene-negative group (P<0.01). As for the neonates with HbA2, the ß-thalassemia gene group had a significantly lower HbA level and significantly higher HbF and HbA2/HbA ratio than the ß-thalassemia gene-negative group (P<0.01). In the neonates without HbA2, HbA, gestational age, and HbA combined with gestational age had an area under the ROC curve (AUC) of 0.865, 0.515, and 0.870, respectively, in determining the presence or absence of ß-thalassemia gene (P<0.01), and HbA and HbA combined with gestational age had a similar AUC and a certain diagnostic value. In the neonates with HbA2, HbA, HbA2/HbA ratio, and HbA combined with HbA2/HbA ratio had an AUC of 0.943, 0.885, and 0.978, respectively, in determining the presence or absence of ß-thalassemia gene. The HbA combined with HbA2/HbA ratio had the largest AUC. In the neonates without HbA2, HbA had the largest AUC in determining the presence or absence of ß-thalassemia gene at the cut-off value of 11.6%, with a sensitivity of 85.81% and a specificity of 79.82%. In the neonates with HbA2, an HbA of 16.1%-22.0% and an HbA2/HbA ratio of >1.4 had the largest AUC in determining the presence or absence of ß-thalassemia gene, with a sensitivity of 91.38% and a specificity of 91.89%. CONCLUSIONS: HbA and HbA2/HbA ratio are effective indices for screening out full-term neonates carrying ß-thalassemia gene.


Assuntos
Talassemia beta , Hemoglobina A2 , Humanos , Recém-Nascido , Programas de Rastreamento , Estudos Retrospectivos , Globinas beta
10.
J Cancer ; 9(18): 3361-3365, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30271497

RESUMO

Background: Several reports have revealed the presence of lymph nodes in the prostatic anterior fat pad (PAFP). To date, no study has described the characteristics of Taiwanese patients harboring PAFP lymph nodes with metastatic prostate cancer involvement. Method: Between December 2006 and May 2015, a total of 849 consecutive patients underwent robot-assisted laparoscopic radical prostatectomy with PAFP dissection. Pathological examination of the dissected PAFP was conducted to assess the presence of lymphoid tissue and prostate cancer involvement. Results: Of the 849 patients, 76 (9.0%) had 1-3 PAFP lymph nodes. Moreover, 11 (1.3%) of the 76 patients had positive lymph node metastases of prostate cancer in the PAFP; 5 (0.6%) of the 11 patients, who had negative pelvic lymph node involvement, were upstaged because of positive metastases in PAFP lymph nodes. Among the 76 patients having PAFP lymph nodes, metastatic lymph nodes were associated with the clinical T stage, preoperative Gleason score, pathological T stage, and pathological N stage (p < 0.001). Patients with pathological seminal vesicle invasion and a higher surgical Gleason score also exhibited PAFP lymph node metastases (p < 0.005). Conclusion: Our data show that 9.0% of patients had PAFP lymph nodes and that 1.3% had prostate cancer metastases. Additionally, 0.6% of patients were upstaged because of positive metastases in PAFP lymph nodes. Because of the pathological analysis of the PAFP, a few patients were upstaged. Thus, routine pathological analysis of the PAFP should only be conducted for those with higher preoperative prostate-specific antigen, higher Gleason score, and advanced T stage observations.

11.
PLoS One ; 13(5): e0196996, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29734364

RESUMO

Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells. In response to different EFS, quantities of detected metabolites in grape cells varied from 6 to 17 in this assay, and 1 to 11 novel metabolites were introduced into metabolome of grape cells. Dual-culture with fungal strains CS2, RH16 and RH5 introduced the highest quantities (10 or 11) of novel metabolites in grape cells. More importantly, the modification of metabolic profiles in grape cells via fungal endophytes appeared to be fungal strain/genus-specificity. Overall, this work revealed that introduction of specific metabolites in host plants may be one consequence during the process of endophytes-host metabolic interactions, which raise the possibility to shape grape qualities and characteristics using tool of fungal endophytes.


Assuntos
Endófitos/metabolismo , Fungos/metabolismo , Metaboloma/genética , Vitis/metabolismo , Cromatografia Líquida de Alta Pressão , Endófitos/patogenicidade , Frutas/metabolismo , Frutas/microbiologia , Fungos/patogenicidade , Filogenia , Vitis/microbiologia
12.
Biomed Pharmacother ; 99: 894-903, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29710489

RESUMO

Hepatocellular carcinoma (HCC) is one of the most common and aggressive malignancies. The current study aimed to investigate the effect of physcion, a major active ingredient in several traditional herbal medicinal plants, for the treatment of HCC. Our data showed that physcion markedly induced apoptosis in human HCC cell lines Huh7 and Bel7402. The pro-apoptotic role of physcion on HCC cells was mediated by mitochondria dysfunction, which was caused by activation of endoplasmic reticulum(ER) stress. Moreover, our findings revealed that physcion stimulated ER stress by activating AMPK signaling. Besides in HCC cell lines, the anti-cancer activity of physcion was also examined in a xenograft mice model, which showed that physcion could significantly suppressed tumor growth. In conclusion, our results indicated that physcion can be considered as a potential chemotherapeutic agent in the treatment of HCC.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Carcinoma Hepatocelular/tratamento farmacológico , Emodina/análogos & derivados , Neoplasias Hepáticas/tratamento farmacológico , Proteínas Quinases Ativadas por AMP/metabolismo , Animais , Apoptose/efeitos dos fármacos , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Emodina/farmacologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Humanos , Neoplasias Hepáticas/patologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Transdução de Sinais/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
13.
Insect Sci ; 2018 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-29645353

RESUMO

Silkworm mutants are valuable resources for both transgenic breeding and gene discovery. PiggyBac-based random insertional mutagenesis has been widely used in gene functional studies. In order to discover genes involved in silk synthesis, a piggyBac-based random insertional library was constructed using Bombyx mori, and the mutants with abnormal cocoon were particularly screened. By this means, a "thin cocoon" mutant was identified. This mutant revealed thinner cocoon shell and shorter posterior silk gland (PSG) compared with the wild type. The messenger RNA (mRNA) levels of all the three fibroin genes, including Fib-H, Fib-L and P25, were significantly down-regulated in the PSG of mutants. Four piggyBac insertion sites were identified in Aquaporin (AQP), Longitudinals lacking protein-like (Lola), Glutamyl aminopeptidase-like (GluAP) and Loc101744460. The mRNA levels of all the four genes were significantly altered in the silk gland of mutants. In particular, the mRNA amount of AQP, a gene responsible for the regulation of osmotic pressure, decreased dramatically immediately prior to the spinning stage in the anterior silk gland of mutants. The identification of the genes disrupted in the "thin cocoon" mutant in this study provided useful information for understanding silk production and transgenic breeding of silkworms in the future.

14.
J Cancer ; 9(2): 321-330, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29344279

RESUMO

Purpose: SET and MYND domain-containing protein2 (SMYD2), a histone lysine methyltransferases, is a candidate human oncogene in multiple tumors. However, the expression dynamics of SMYD2 in hepatocellular carcinoma (HCC) and its clinical/prognostic significance are unclear. Methods: The SMYD2 expression profile was examined by quantitative real-time polymerase chain reaction (qRT-PCR), and immunohistochemistry (IHC) in HCC tissues and matched adjacent non-tumorous tissues. SMYD2 was silenced in HCC cell lines to determine its role in tumor proliferation and cell cycle progression, and the possible mechanism. Spearman's rank correlation, Kaplan-Meier plots and Cox proportional hazards regression model were used to analyze the data. Results: The SMYD2 expression in HCC tissues were significantly up-regulated at both mRNA and protein levels as compared with the matched adjacent non-tumorous tissues. By IHC, positive expression of SMYD2 was examined in 122/163 (74.85%) of HCC and in 10/59 (16.95%) of tumor-adjacent tissues. Positive expression of SMYD2 was correlated with tumor size, vascular invasion, differentiation and TNM stage (P < 0.05). In univariate survival analysis, a significant association between positive expression of SMYD2 and shortened patients' survival was found (P < 0.05). Importantly, SMYD2 expression together with vascular invasion (P < 0.05) provided significant independent prognostic parameters in multivariate analysis. Functionally, SMYD2 silenced markedly inhibited cell proliferation and cell cycle progression in SMMC-7721 cell. Conclusions: Our findings provide evidences that positive expression of SMYD2 in HCC may be important in the acquisition of an aggressive phenotype, and it is an independent biomarker for poor prognosis of patients with HCC.

15.
J Clin Nurs ; 27(15-16): 3008-3019, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29314311

RESUMO

AIMS AND OBJECTIVES: To establish evidence-based nursing-sensitive quality indicators for emergency nursing in China. BACKGROUND: China lacks nursing-sensitive quality indicators necessary for assessing the quality of emergency nursing and essential to nursing management. DESIGN: Prospective. METHODS: A literature search for relevant evidence-based studies was performed using several databases from January 2009-May 2014. Previously reported quality indicators were identified as appropriate for assessment by a panel of 40 experts in emergency medicine and nursing. Two successive rounds of Delphi surveys were conducted using questionnaires designed by the experts. Kendal's W coordination coefficients were calculated for indicator importance, rationality of calculation and feasibility of data collection. RESULTS: Thirty-three quality indicators were initially proposed for expert evaluation. After round 1 of expert discussion, Kendal's W coordination coefficients were .152 for importance, .092 for rationality and .141 for feasibility of data collection (all p < .001). Seven unsuitable items were discarded in round 1 and 11 discarded in round 2, which also added one new item. Finally, the experts reached consensus on 16 items established as appropriate nursing-sensitive quality indicators for emergency nursing care. CONCLUSION: Evidence-based nursing-sensitive quality indicators were established through a consensus of experts in emergency nursing and medicine. RELEVANCE TO CLINICAL PRACTICE: The current findings may provide a theoretical basis for establishing an emergency nursing quality database and improving the quality of emergency nursing care in China.


Assuntos
Enfermagem em Emergência/normas , Enfermagem Baseada em Evidências/normas , Indicadores de Qualidade em Assistência à Saúde/normas , China , Consenso , Técnica Delfos , Humanos , Estudos Prospectivos , Inquéritos e Questionários
16.
Ann Lab Med ; 38(1): 9-16, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29071813

RESUMO

BACKGROUND: Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine with chemokine-like functions, has been shown to play a central role in several acute and chronic inflammatory diseases. However, limited information is available regarding the use of MIF as an inflammatory pathway marker in patients with tuberculosis. This study aimed to investigate the association of MIF with IFN-γ and TNF-α in active pulmonary tuberculosis (APTB) following anti-tuberculosis treatment. METHODS: The MIF, TNF-α, and IFN-γ serum levels were determined in 47 patients with APTB by cytokine-specific ELISA at four phases: prior to anti-tuberculosis drug treatment (baseline), and following 2, 4, and 6 months of treatment. In addition, we measured the MIF, TNF-α, and IFN-γ serum levels in 50 health controls. RESULTS: MIF serum levels were significantly elevated (P<0.05) in patients with APTB prior to treatment compared with that in control subjects, and TNF-α ≥449.7 pg/mL was associated with high MIF levels (≥13.1 ng/mL). MIF levels were significantly reduced (P<0.01) following 2, 4, and 6 months of treatment, with variations in TNF-α and IFN-γ serum levels. MIF levels were positively correlated with the paired TNF-α level at baseline (r=0.1103, P=0.0316) and following 6 months of treatment (r=0.09569, P=0.0364). CONCLUSIONS: A reduction in the MIF serum levels in patients with APTB following anti-tuberculosis treatment may positively affect host immune protection against Mycobacterium tuberculosis infection. Thus, serum MIF levels may constitute a useful marker for assessing therapy effectiveness in patients with APTB.


Assuntos
Biomarcadores/sangue , Fatores Inibidores da Migração de Macrófagos/sangue , Tuberculose Pulmonar/diagnóstico , Doença Aguda , Adulto , Idoso , Antituberculosos/uso terapêutico , Estudos de Casos e Controles , Citocinas/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Interferon gama/sangue , Masculino , Pessoa de Meia-Idade , Razão de Chances , Fatores de Risco , Tuberculose Pulmonar/tratamento farmacológico , Fator de Necrose Tumoral alfa/sangue , Regulação para Cima
17.
Acta Biomater ; 65: 272-282, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29037896

RESUMO

Nanotopography plays a pivotal role in the regulation of cellular responses. Nonetheless, little is known about how the gradient size of nanostructural stimuli alters the responses of endothelial progenitor cells without chemical factors. Herein, the fabrication of gradient nanopattern plates intended to mimic microenvironment nanotopography is described. The gradient nanopattern plates consist of nanopillars of increasing diameter ranges [120-200 nm (GP 120/200), 200-280 nm (GP 200/280), and 280-360 nm (GP 280/360)] that were used to screen the responses of human endothelial colony-forming cells (hECFCs). Nanopillars with a smaller nanopillar diameter caused the cell area and perimeter of hECFCs to decrease and their filopodial outgrowth to increase. The structure of vinculin (a focal adhesion marker in hECFCs) was also modulated by nanostructural stimuli of the gradient nanopattern plates. Moreover, Rho-associated protein kinase (ROCK) gene expression was significantly higher in hECFCs cultured on GP 120/200 than in those on flat plates (no nanopillars), and ROCK suppression impaired the nanostructural-stimuli-induced vinculin assembly. These results suggest that the gradient nanopattern plates generate size-specific nanostructural stimuli suitable for manipulation of the response of hECFCs, in a process dependent on ROCK signaling. This is the first evidence of size-specific nanostructure-sensing behavior of hECFCs. SIGNIFICANCE: Nano feature surfaces are of growing interest as materials for a controlled response of various cells. In this study, we successfully fabricated gradient nanopattern plates to manipulate the response of blood-derived hECFCs without any chemical stimulation. Interestingly, we find that the sensitive nanopillar size for manipulation of hECFCs is range between 120 nm and 200 nm, which decreased the area and increased the filopodial outgrowth of hECFCs. Furthermore, we only modulate the nanopillar size to increase ROCK expression can be an attractive method for modulating the cytoskeletal integrity and focal adhesion of hECFCs.


Assuntos
Células Endoteliais/citologia , Adesões Focais , Nanoestruturas , Células-Tronco/citologia , Actinas/metabolismo , Adulto , Animais , Western Blotting , Células Cultivadas , Células Endoteliais/metabolismo , Matriz Extracelular/metabolismo , Humanos , Masculino , Microscopia Eletrônica de Transmissão , Pessoa de Meia-Idade , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais , Células-Tronco/metabolismo , Vinculina/metabolismo , Quinases Associadas a rho/genética , Quinases Associadas a rho/metabolismo
18.
Insect Sci ; 2017 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-29080335

RESUMO

Juvenile hormone (JH) is one of key insect hormones that regulate metamorphosis. Juvenile hormone diol kinase (JHDK) is an enzyme involved in JH metabolism and catalyzes JH diol to form a polar end product, JH diol phosphate that has no JH activity. In this study, a JHDK cDNA was cloned from Spodoptera litura and the structure and expression of the gene was characterized. The cDNA was 714 base pairs in length and encoded a protein of 183 amino acids with a molecular mass of 21 kDa and a pI of 4.55. Based on the structure three putative calcium binding motifs and GTP-binding motifs were predicted in the protein. Modeling of the 3-D structure showed that the protein consisted of eight α-helixes linked with loops, with no ß-sheets. The gene was expressed in the epidermis, fat body and midgut of 5th and 6th instar larvae. The expression level in the epidermis was lower than in the fat body and midgut. The gene was expressed at higher levels at the early stages than in the later stages of 5th and 6th instar midgut and fat body. The results suggest that this gene may be involved in the regulation of the JH titer in larvae of S. litura. This article is protected by copyright. All rights reserved.

19.
World J Gastroenterol ; 23(29): 5345-5355, 2017 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-28839434

RESUMO

AIM: To clarify the mechanisms of connexin 32 (Cx32) downregulation by potential transcriptional factors (TFs) in Helicobacter pylori (H. pylori)-associated gastric carcinogenesis. METHODS: Approximately 25 specimens at each developmental stage of gastric carcinogenesis [non-atrophic gastritis, chronic atrophic gastritis, intestinal metaplasia, dysplasia and gastric carcinoma (GC)] with H. pylori infection [H. pylori (+)] and 25 normal gastric mucosa (NGM) without H. pylori infection [H. pylori (-)] were collected. After transcriptional factor array analysis, the Cx32 and PBX1 expression levels of H. pylori-infected tissues from the developmental stages of GC and NGM with no H. pylori infection were measured by real-time polymerase chain reaction (RT-PCR) and Western blot analysis. Regarding H. pylori-infected animal models, the Cx32 and PBX1 mRNA expression levels and correlation between the gastric mucosa from 10 Mongolian gerbils with long-term H. pylori colonization and 10 controls were analyzed. PBX1 and Cx32 mRNA and protein levels were further studied under the H. pylori-infected condition as well as PBX1 overexpression and knockdown conditions in vitro. RESULTS: Incremental PBX1 was first detected by TF microarray in H. pylori-related gastric carcinogenesis. The identical trend of PBX1 and Cx32 expression was confirmed in the developmental stages of H. pylori-related clinical specimens. The negative correlation of PBX1 and Cx32 was confirmed in H. pylori-infected Mongolian gerbils. Furthermore, decreased PBX1 expression was detected in the normal gastric epithelial cell line GES-1 with H. pylori infection. Enforced overexpression or RNAi-mediated knockdown of PBX1 contributed to the diminished or restored Cx32 expression in GES-1 and the gastric carcinoma cell line BGC823, respectively. Finally, dual-luciferase reporter assay in HEK293T cells showed that Cx32 promoter activity decreased by 30% after PBX1 vector co-transfection, indicating PBX1 as a transcriptional downregulator of Cx32 by directly binding to its promoters. CONCLUSION: PBX1 is one of the determinants in the Cx32 promoter targeting site, preventing further damage of gap junction protein in H. pylori-associated gastric carcinogenesis.


Assuntos
Carcinogênese/patologia , Carcinoma/patologia , Conexinas/metabolismo , Infecções por Helicobacter/patologia , Fator de Transcrição 1 de Leucemia de Células Pré-B/metabolismo , Neoplasias Gástricas/patologia , Adolescente , Adulto , Idoso , Animais , Carcinoma/microbiologia , Linhagem Celular Tumoral , Doença Crônica , Técnicas de Cocultura , Conexinas/genética , Modelos Animais de Doenças , Regulação para Baixo , Feminino , Mucosa Gástrica/microbiologia , Mucosa Gástrica/patologia , Gastrite Atrófica/microbiologia , Gastrite Atrófica/patologia , Gastroscopia , Técnicas de Silenciamento de Genes , Gerbillinae , Células HEK293 , Infecções por Helicobacter/microbiologia , Helicobacter pylori/isolamento & purificação , Humanos , Masculino , Metaplasia , Pessoa de Meia-Idade , Fator de Transcrição 1 de Leucemia de Células Pré-B/genética , Regiões Promotoras Genéticas/genética , RNA Interferente Pequeno/metabolismo , Estômago/microbiologia , Estômago/patologia , Neoplasias Gástricas/microbiologia , Neoplasias Gástricas/cirurgia , Regulação para Cima , Adulto Jovem
20.
J Epidemiol ; 27(11): 516-523, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28576446

RESUMO

BACKGROUND: Phthalate diesters are commonly used and have been well established as environmental endocrine disruptors. However, few studies have examined their effects on sex steroid hormones in children. We followed children over time to examine the association between pre- and post-natal phthalate exposure and sex steroid hormone levels at 2, 5, 8, and 11 years of age. METHODS: We recruited 430 pregnant women from central Taiwan from 2000 to 2001 and assessed their children at birth, 2, 5, 8, and 11 years of age. We studies children with at least one measurement for both phthalate and hormone levels during each any of the follow-up time point (n = 193). Estradiol, free testosterone, testosterone, and progesterone were measured from venous blood. Three monoesters of di-2-ethylhexyl phthalate (DEHP), mono-benzyl phthalate, mono-n-butyl phthalate, mono-ethyl phthalate, and mono-methyl phthalate were measured in maternal urine collected during the 3rd trimester and child urine collected at each follow-up point. The sum of mono-2-ethylhexyl phthalate (∑MEHP) was calculated by summing the concentrations of the three DEHP monoesters. Generalized estimating equation regression analysis with repeated measures was used to estimate associations between phthalate metabolites and hormone levels. RESULTS: After adjustment for potential confounders, maternal ∑MEHP level was associated with decreased levels of progesterone in girls (ß = -0.309 p = 0.001). The child ∑MEHP concentration was associated with decreased levels of progesterone for girls (ß = -0.194, p = 0.003) and with decreased levels of free testosterone for boys (ß = -0.124, p = 0.004). CONCLUSIONS: Early-life DEHP exposure may alter sex steroid hormones of children over time, which may pose potential reproductive health risks.


Assuntos
Exposição Ambiental/efeitos adversos , Hormônios Esteroides Gonadais/sangue , Ácidos Ftálicos/toxicidade , Ácidos Ftálicos/urina , Efeitos Tardios da Exposição Pré-Natal , Adulto , Criança , Pré-Escolar , Estudos de Coortes , Feminino , Humanos , Masculino , Projetos Piloto , Gravidez , Taiwan
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