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1.
J Hazard Mater ; 424(Pt B): 127354, 2021 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-34634699

RESUMO

Due to its wide applications in tire and rubber products, carbon black (CB) implicates concerns on its safety during production, collection, and handling. Here we report that exposure CB, increases coagulation-thrombosis potential in a splenic extramedullary hemopoiesis (EMH)-dependent manner. Adult C57BL/6 mice are kept in whole-body inhalation chambers, and exposed to filtered room air (FRA) or CB for 28 consecutive days. CB exposure resulted in splenic EMH characterized with platelet precursor cells, megakaryocytes (MKs), hyperplasia and enhanced in vivo blood coagulation ability. Metabolomics analysis suggests significant enhance in PGE2 production but reduction in folic acid (FA) levels in murine serum following CB exposure. Mechanistically, activation of COX-dependent PGE2 production promotes IL-6 expression in splenic macrophages, which subsequently results in splenic EMH and increased platelet counts in circulation. Administration of FA protects the mice against CB-induced splenic EMH through inhibiting prostaglandin-endoperoxide synthase 2 (Ptgs2 or Cox2) and prostaglandin E synthase (Ptges) expression in splenic macrophages, eventually recover the coagulation capacity to normal level. The results strongly suggest the involvement of splenic EMH in response to CB exposure and subsequently increased coagulation-thrombosis potential. Supplementation with FA may be a candidate to prevent thrombosis potential attributable to CB exposure.

2.
Ann Palliat Med ; 10(8): 9078-9087, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34488393

RESUMO

BACKGROUND: Periodontitis (PD) is a chronic inflammatory disease caused by infection of the periodontal supporting tissues. Clinical studies have reported that rheumatoid arthritis (RA) patients have a higher prevalence of PD. This study aimed to explore the correlation between RA and PD. METHODS: A total of 307 RA patients (RA group) and 324 healthy individuals (control group) who received physical examinations during the same period were recruited to this study. The incidence of PD in the two groups was analyzed, and the periodontal disease index (PDI) and bleeding on probing (BOP) were recorded. Then, 42 RA patients with PD and 56 control group patients with PD were selected for further analysis. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of interleukin 1ß (IL-1ß) and tumor necrosis factor-α (TNF-α) in the gingival crevicular fluid (GCF) of the two groups. For patients with both RA and PD, the level of serum C-reactive protein (CRP) and the duration of morning stiffness were also recorded. RESULTS: The prevalence of PD in the RA group (51.5%) was significantly higher than that in the control group (31.2%), and the prevalence of PD also increased notably with the increase of age and the duration of the disease in RA patients. The levels of TNF-α and IL-1ß in the PDI and the GCF in the concurrent RA and PD group were significantly higher than those in the PD group (P<0.05). Partial correlation analysis showed that TNF-α in the GCF positively correlated with the BOP of patients with RA and PD. Multiple linear regression analysis showed that the level of TNF-α in the GCF and serum CRP were independent influencing factors of the level of IL-1ß in the GCF (the r values were 1.074 and 3.851, respectively; P<0.01). CONCLUSIONS: The presence of RA can increase risk of PD occurrence and is positively correlated with the levels of IL-1ß and TNF-α in the GCF.


Assuntos
Artrite Reumatoide , Periodontite , Líquido do Sulco Gengival/química , Humanos , Interleucina-1beta/análise , Periodontite/epidemiologia , Fator de Necrose Tumoral alfa
3.
J Hazard Mater ; 416: 125878, 2021 08 15.
Artigo em Inglês | MEDLINE | ID: mdl-34492818

RESUMO

With the increased appreciation for the significance of noncoding RNAs (ncRNAs), the present research aimed to determine the role of competing endogenous RNA (ceRNA) in the process of particulate matter (PM) exposure-induced pulmonary damage. Alterations in messenger RNA (RNA), microRNA and long non-coding RNA (lncRNA) profiles of human bronchial epithelial (HBE) cells treated with PM were analyzed by microarray assays. Next, we identified that lncRNA taurine upregulated gene 1 (TUG1) acted as a competing endogenous RNA for microRNA-222-3p (miR-222-3p) and subsequently attenuated the inhibitory effect of miR-222-3p on CUGBP elav-like family member 1 (CELF1). The binding potency among ceRNAs was verified by RNA immunoprecipitation (RIP) assay and dual-luciferase reporter assay. Knockdown of TUG1 attenuated HBE cell apoptosis and cell cycle arrest by downregulation of CELF1 and protein 53 (p53). Further, we confirmed that Tug1/mir-222-3p/CELF1/p53 network aggravated PM-induced airway hyper-reactivity (AHR) in mice. In summary, our novel findings revealed that TUG1 triggered dysfunction of pulmonary cells followed by PM exposure by serving as a sponge for miR-222-3p and thereby upregulating the expression of CELF1and p53.


Assuntos
MicroRNAs , RNA Longo não Codificante , Animais , Proliferação de Células , Camundongos , MicroRNAs/genética , Material Particulado/toxicidade , RNA Longo não Codificante/genética , Taurina
4.
Sci Rep ; 11(1): 18201, 2021 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-34521875

RESUMO

Panax ginseng is one of the oldest and most generally prescribed herbs in Eastern traditional medicine to treat diseases. Several studies had documented that ginseng leaves have anti-oxidative, anti-inflammatory, and anticancer properties similar to those of ginseng root. The aim of this research was to forecast of the molecular mechanism of ginseng leaves on lung cancer by molecular docking and network pharmacology so as to decipher ginseng leaves' entire mechanism. The compounds associated with ginseng leaves were searched by TCMSP. TCMSP and Swiss Target Prediction databases were used to sort out the potential targets of the main chemical components. Targets were collected from OMIM, PharmGKB, TTD, DrugBank and GeneCards which related to immunity and lung cancer. Ginseng leaves exert its lung cancer suppressive function by regulating the several signaling proteins, such as JUN, STAT3, AKT1, TNF, MAPK1, TP53. GO and KEGG analyses indicated that the immunoreaction against lung cancer by ginseng leaves might be related to response to lipopolysaccharide, response to oxidative stress, PI3K-Akt, MAPK and TNF pathway. Molecular docking analysis demonstrated that hydrogen bonding was interaction's core forms. The results of CCK8 test and qRT-PCR showed that ginseng leaves inhibit cell proliferation and regulates AKT1 and P53 expression in A549. The present study clarifies the mechanism of Ginseng leaves against lung cancer and provides evidence to support its clinical use.

5.
Curr Med Sci ; 41(2): 211-218, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33877537

RESUMO

The association between meteorological factors and infectious diarrhea has been widely studied in many countries. However, investigation among children under 5 years old in Jiangsu, China remains quite limited. Data including infectious diarrhea cases among children under five years old and daily meteorological indexes in Jiangsu, China from 2015 to 2019 were collected. The lag-effects up to 21 days of daily maximum temperature (Tmax) on infectious diarrhea were explored using a quasi-Poisson regression with a distributed lag non-linear model (DLNM) approach. The cases number of infectious diarrhea was significantly associated with seasonal variation of meteorological factors, and the burden of disease mainly occurred among children aged 0-2 years old. Moreover, when the reference value was set at 16.7°C, Tmax had a significant lag-effect on cases of infectious diarrhea among children under 5 years old in Jiangsu Province, which was increased remarkably in cold weather with the highest risk at 8°C. The results of DLNM analysis implicated that the lag-effect of Tmax varied among the 13 cities in Jiangsu and had significant differences in 8 cities. The highest risk of Tmax was presented at 5 lag days in Huaian with a maximum RR of 1.18 (95% CI: 1.09, 1.29). Suzhou which had the highest number of diarrhea cases (15830 cases), had a maximum RR of 1.04 (95% CI:1.03, 1.05) on lag 15 days. Tmax is a considerable indicator to predict the epidemic of infectious diarrhea among 13 cities in Jiangsu, which reminds us that in cold seasons, more preventive strategies and measures should be done to prevent infectious diarrhea.

6.
Oral Dis ; 27(3): 600-610, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32762066

RESUMO

AIM: This study was aimed to investigate the role of TUG1 in LPS-stimulated hPDLCs and to evaluate the potential functions of TUG1 in the pathogenesis of periodontitis. METHODS: LPS-stimulated hPDLCs were established as the cell model. CCK-8 assay was performed to assess cell proliferation ability. Flow cytometry was performed to detect cell cycle distribution, and quantitative RT-PCR and Western blotting were conducted to measure gene expressions. ELISA kits were used to evaluate the production of inflammatory cytokines. The putative binding site between TUG1 and miR-498 was verified using luciferase reporter and RNA immunoprecipitation assays. RESULTS: TUG1 was downregulated upon LPS stimulation in hPDLCs. TUG1 overexpression promoted cell proliferation through regulating the cell cycle distribution, along with the decreased expression of p21 and increased expression of CDK2 and cyclin D1. Besides, TUG1 overexpression decreased the production of inflammatory cytokines. The effects were opposite upon TUG1 knockdown. TUG1 negatively regulated its target miR-498, and influenced the expression of RORA, the direct target of miR-498. Simultaneous TUG1 overexpression and miR-498 reversed the effect of TUG1 overexpression alone on alleviating LPS-induced cell injury and inhibition of Wnt/ß-catenin signaling, which was further changeover after co-overexpression with RORA. CONCLUSION: Therefore, TUG1 could protect against periodontitis via regulating miR-498/RORA mediated Wnt/ß-catenin signaling.


Assuntos
MicroRNAs , Periodontite , RNA Longo não Codificante , Apoptose , Proliferação de Células , Humanos , Lipopolissacarídeos , MicroRNAs/genética , Membro 1 do Grupo F da Subfamília 1 de Receptores Nucleares , RNA Longo não Codificante/genética
7.
Environ Pollut ; 256: 113465, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31679870

RESUMO

Fulvic acid (FA) significantly influences the bioavailability and fate of heavy metals in environments, while its acid-base characters and metal binding processes are still unclear. Here, spectroscopic techniques combined with multiple models (e.g., NICA-Donnan model) and two-dimensional correlation spectroscopy (2D COS) were applied to explore the proton and copper binding properties of FA sub-fractions (FA3-FA13). The charge densities, average contents of carboxylic and phenolic groups, average dissociation constants pKa1 and pKa2 of sub-fractions ranged 0-16 meq∙g∙C-1, 5.03-9.58 meq∙g∙C-1, 2.52-4.67 meq∙g∙C-1, 4.15-4.33 and 8.52-9.72, respectively. FA sub-fractions had a relatively narrow distribution of carboxyl group and a broad distribution of phenolic group. FA sub-fractions also exhibited roughly two phenolic hydroxyl groups per every 1-3 phenyl rings. Differential absorbance spectra (DAS) derived Gaussian bands were associated to the inter-chromophore interactions, the changes of molecular conformations and functional groups with copper addition. Differential spectra slopes (DSlope275-295&325-375) were more significant with higher copper concentration and copper amounts bonded to carboxylic groups. UV-Vis and fluorescence spectra with 2D heterospectral COS revealed the copper binding heterogeneities and sequential orders of chromophores and fluorophores, quantitatively confirming by the order of conditional stability constants (log KCu: 4.64-5.56). Salicylic-/polyhydroxyphenolic, hydroxyl and amino groups were strongly associated to the basic units for fluorophores. Sequential changes followed the order of humic-like→fulvic-like materials for FA3/FA5, humic-like→fulvic-like→tryptophan-like materials for FA7, and humic-like→tryptophan-like→fulvic-like→tyrosine-like materials for FA9/FA13. Spectroscopic techniques combined with various models (especially for 2D COS) are beneficial to elucidate the binding heterogeneity and sensitivity for metal-organic matters at the functional group level.


Assuntos
Benzopiranos/análise , Cobre/análise , Modelos Químicos , Cobre/química , Substâncias Húmicas/análise , Metais Pesados , Modelos Teóricos , Prótons , Espectrometria de Fluorescência
8.
Pak J Pharm Sci ; 29(4 Suppl): 1457-9, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27592480

RESUMO

To investigate the surveillance of drug resistance and serotype monitoring of steptococcus pneumoniae in hospitalized children. the pathogenic bacteria isolation and identification methods were employed to do the bacteria isolation identification and drug sensitive test on the specimens from Women & Infants Hospital of Zhengzhou. From the specimens, there were 134 detected strains of Streptococcus pneumoniae, and the drug resistance to erythromycin and clindamycin were respectively 97.7% and 89.9%, and the drug resistance to tetracycline, azithromycin and paediatric compound sulfamethoxazole were respectively 86. 3%, 58. 3%, 51. 2%. The vancomycin resistant Streptococcus pneumoniae were often not found. the Streptococcus pneumoniae in children were generally with drug resistant in Zhengzhou area. It shall strengthen drug resistance surveillance, and reasonably choose antibacterial agents.


Assuntos
Antibacterianos/farmacologia , Infecções Pneumocócicas/microbiologia , Streptococcus pneumoniae/efeitos dos fármacos , Criança , Combinação de Medicamentos , Farmacorresistência Bacteriana , Hospitalização , Humanos , Testes de Sensibilidade Microbiana
9.
Pak J Pharm Sci ; 29(1 Suppl): 339-41, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27005510

RESUMO

In this paper, the clinical value of the detection about serum and unconjugated bilirubin (UCB) in neonatal jaundice was studied to found an effective and rapid method for diagnose of neonatal jaundice. ALB (Serum Albumin), total serum bilirubin (TSB) and UCB were detected by ELISA method among the 100 cases with neonatal jaundice selected for the study. The values of ALB, UCB and TSB in moderate jaundice patients were (42.83±3.87) g/L, (287.35±44.38) µm/L, (304.16±43.40) µm/L, respectively; as for the severe jaundice patients, the values were (38.41±4.82) g/L, (354.38±48.75) µm/L, (375.20±47.51) µm/L. The results showed significant differences with the p< 0.05 between moderate and severe jaundice patients. The level of ALB, UCB, TSB in hemolytic jaundice, obstructive jaundice and jaundice caused by other infections also had significant differences, and the difference was statistically significant (p<0.05). The detection of ALB and UCB provides a useful method for the diagnosis and assessment of neonatal jaundice.


Assuntos
Bilirrubina/sangue , Icterícia Neonatal/diagnóstico , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Recém-Nascido , Icterícia/sangue , Icterícia/diagnóstico , Icterícia Neonatal/sangue , Icterícia Neonatal/etiologia , Icterícia Obstrutiva/sangue , Icterícia Obstrutiva/diagnóstico , Masculino , Valor Preditivo dos Testes , Albumina Sérica/análise
10.
Zhongguo Yi Liao Qi Xie Za Zhi ; 38(2): 98-101, 2014 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-24941770

RESUMO

The finite element method was used for simulating the mechanical performance and fatigue safety of three different structures of Nitinol stent. According to the actual situation, after proposing reasonable assumptions and simplification, the geometry model and finite element model establishment, material mode selection and boundary condition setting are completed. The strain and fatigue life of different stent edges wide (omega) or strut angle (theta) are computed. The result can provide a valuable reference for the optimal design of stent.


Assuntos
Ligas , Análise de Elementos Finitos , Desenho de Prótese , Stents
11.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 5): m596, 2011 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-21754316

RESUMO

In the title compound, [CuCl(2)(C(18)H(16)N(2)O(2))], the Cu(II) atom lies on a twofold axis and is six-coordinated in a distorted octa-hedral environment defined by two N and two O atoms from the ligand and by two Cl atoms. In the crystal, π-π inter-actions [centroid-centroid distance = 3.838 (1) Å] and C-H⋯Cl hydrogen bonds link adjacent mol-ecules into a chain structure along [101].

12.
Acta Crystallogr Sect E Struct Rep Online ; 67(Pt 5): m598, 2011 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-21754318

RESUMO

In the title compound, [Co(NO(3))(2)(C(18)H(16)N(2)O(2))], the Co(II) ion is six-coordinated in a distorted octa-hedral environment defined by two O and two N atoms from the ligand and by two O atoms from two nitrate anions. A two-dimensional network parallel to the ab plane is built up by C-H⋯O hydrogen bonds, which link adjacent mol-ecules in the crystal structure.

13.
Zhongguo Dang Dai Er Ke Za Zhi ; 12(6): 429-32, 2010 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-20540849

RESUMO

OBJECTIVE: To investigate the distribution and frequency of UGTIA6 A541G genetic polymorphism in Han epileptic children from Henan and to evaluate the effect of UGTIA6 A541G genetic polymorphism on serum concentrations of valproic acid. METHODS: The method of gas chromatography was used to assay serum concentrations of valproic acid. UGTIA6 A541G genetic polymorphism was screened by PCR-RFLP. Direct sequencing was used to confirm the expected sequences of each genotype. RESULTS: The genotypic frequencies of UGTIA6 A541G were as follows: AA in 76 cases, AG in 65 cases and GG in 6 cases. The mean values of serum concentrations of valproic acid in patients with A541G AA, AG and GG were 3.91+/-1.57, 3.59+/-1.39 and 3.73+/-1.28 microg/mL, respectively (dose-adjusted trough concentration on a mg/kg basis). There were no significant differences in serum concentrations of valproic acid among the three groups. CONCLUSIONS: UGT1A6 A541G gene polymorphism does not influence serum concentrations of valproic acid in Han epileptic children. Individual differences in serum concentrations of valproic acid may be attributed to many factors.


Assuntos
Anticonvulsivantes/metabolismo , Epilepsia/genética , Glucuronosiltransferase/genética , Polimorfismo Genético , Ácido Valproico/metabolismo , Adolescente , Criança , Pré-Escolar , China/etnologia , Epilepsia/tratamento farmacológico , Epilepsia/metabolismo , Humanos
14.
Artigo em Inglês | MEDLINE | ID: mdl-19294673

RESUMO

BACKGROUND: Recent data have demonstrated that treatment with sodium benzoate (SB) leads to significant developmental defects in motor neuron axons and neuromuscular junctions in zebrafish larvae, thereby implying that SB can be neurotoxic. This study examined whether SB affects the development of dopaminergic neurons in the zebrafish brain. METHODS: Zebrafish embryos were exposed to different concentrations of SB for various durations, during which the survival rates were recorded, the expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the neurons in the ventral diencephalon were detected by in situ hybridization and immunofluorescence, and the locomotor activity of larval zebrafish was measured. RESULTS: The survival rates were significantly decreased with the increase of duration and dose of SB-treatment. Compared to untreated clutch mates (untreated controls), treatment with SB significantly downregulated expression of TH and DAT in neurons in the ventral diencephalon of 3-day post-fertilization (dpf) zebrafish embryos in a dose-dependent manner. Furthermore, there was a marked decrease in locomotor activity in zebrafish larvae at 6dpf in response to SB treatment. CONCLUSIONS: The results suggest that SB exposure can cause significantly decreased survival rates of zebrafish embryos in a time- and dose-dependent manner and downregulated expression of TH and DAT in dopaminergic neurons in the zebrafish ventral diencephalon, which results in decreased locomotor activity of zebrafish larvae. This study may provide some important information for further elucidating the mechanism underlying SB-induced developmental neurotoxicity.


Assuntos
Diencéfalo/efeitos dos fármacos , Proteínas da Membrana Plasmática de Transporte de Dopamina/biossíntese , Conservantes de Alimentos/toxicidade , Neurônios/efeitos dos fármacos , Benzoato de Sódio/toxicidade , Tirosina 3-Mono-Oxigenase/biossíntese , Proteínas de Peixe-Zebra/biossíntese , Peixe-Zebra/embriologia , Animais , Sequência de Bases , DNA Complementar/genética , Diencéfalo/embriologia , Diencéfalo/metabolismo , Proteínas da Membrana Plasmática de Transporte de Dopamina/genética , Relação Dose-Resposta a Droga , Regulação para Baixo/efeitos dos fármacos , Indução Enzimática/efeitos dos fármacos , Hibridização In Situ , Larva/efeitos dos fármacos , Microscopia de Fluorescência , Dados de Sequência Molecular , Neurônios/metabolismo , Distribuição Aleatória , Natação , Tirosina 3-Mono-Oxigenase/genética , Proteínas de Peixe-Zebra/genética
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